EP3505253A1 - Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür - Google Patents

Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür Download PDF

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Publication number
EP3505253A1
EP3505253A1 EP18215536.6A EP18215536A EP3505253A1 EP 3505253 A1 EP3505253 A1 EP 3505253A1 EP 18215536 A EP18215536 A EP 18215536A EP 3505253 A1 EP3505253 A1 EP 3505253A1
Authority
EP
European Patent Office
Prior art keywords
compartment
waste
liquid
reaction
path
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18215536.6A
Other languages
English (en)
French (fr)
Other versions
EP3505253B1 (de
Inventor
Wei-Yu Chung
Song-Bin Huang
Shing-Lun Liu
Yu-Kai Kao
Yi-Chen Li
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Delta Electronics Inc
Original Assignee
Delta Electronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201811535018.6A external-priority patent/CN110004141A/zh
Application filed by Delta Electronics Inc filed Critical Delta Electronics Inc
Publication of EP3505253A1 publication Critical patent/EP3505253A1/de
Application granted granted Critical
Publication of EP3505253B1 publication Critical patent/EP3505253B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/028Modular arrangements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14Process control and prevention of errors
    • B01L2200/141Preventing contamination, tampering
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16Reagents, handling or storing thereof
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics

Definitions

  • the present invention relates to an extraction cassette, and in particular to an extraction cassette with an extraction module.
  • the conventional extraction module is only for extracting nucleic acid, which cannot receive waste-liquid. Since the waste-liquid may pollute the filter inside the reaction compartment, the conventional waste-liquid compartment is far away from the collection compartment.
  • the waste-liquid compartment and the reaction compartment are disposed on different modules, and the size and cost of the conventional extraction cassette are increased.
  • an all-in-one extraction module which extracts nucleic acid by the magnetic bead extraction technology.
  • the efficiency and purity of the magnetic bead extraction technology are unqualified.
  • an extraction cassette in one embodiment, includes a liquid receiving module and an extraction module.
  • the extraction module communicates with the liquid receiving module.
  • the extraction module includes an extraction module body, an expansion compartment, a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment.
  • the expansion compartment is formed on the extraction module body.
  • the reaction compartment is formed on the extraction module body, wherein the reaction compartment includes a reaction compartment inlet, a reaction compartment outlet and a reaction compartment notch, the expansion compartment is connected to the reaction compartment notch, and the reaction compartment notch is located between the reaction compartment inlet and the reaction compartment outlet.
  • the filter is disposed in the reaction compartment and corresponding to the reaction compartment outlet.
  • the collection compartment is formed on the extraction module body and communicates with the reaction compartment outlet.
  • the first waste-liquid compartment is formed on the extraction module body, wherein the first waste-liquid compartment communicates with the reaction compartment outlet.
  • the second waste-liquid compartment is formed on the extraction module body, wherein the second waste-liquid compartment communicates with the reaction compartment outlet.
  • the reaction compartment includes a cone-shaped portion, the filter is disposed on the cone-shaped portion, and the reaction compartment outlet is formed on one end of the cone-shaped portion.
  • the extraction module further includes a first path and a second path, the first path connects the reaction compartment outlet to the first waste-liquid compartment and the second waste-liquid compartment, and the second path connects the reaction compartment outlet to the collection compartment.
  • the first path intersects the second path at the reaction compartment outlet, a stopper wall is formed in the second path, and the stopper wall is formed on one end of the second path and is adjacent to the reaction compartment outlet.
  • the extraction module further includes a third path and a fourth path, the third path connects the first path to the first waste-liquid compartment, and the fourth path connects the first path to the second waste-liquid compartment.
  • the first waste-liquid compartment includes a first waste-liquid compartment connection hole
  • the second waste-liquid compartment includes a second waste-liquid compartment connection hole
  • the third path connects the first path to the first waste-liquid compartment connection hole
  • the fourth path connects the first path to the second waste-liquid compartment connection hole
  • at least one portion of the second waste-liquid compartment is located between the first waste-liquid compartment connection hole and the second waste-liquid compartment connection hole.
  • At least one portion of the fourth path extends in a first direction, and the first direction is away from the first waste-liquid compartment.
  • the second waste-liquid compartment includes a second waste-liquid compartment pressure hole, and at least one portion of the waste-liquid compartment is located between the first waste-liquid compartment and the second waste-liquid compartment pressure hole.
  • At least one portion of the second path extends in the first direction.
  • the collection compartment includes a collection compartment pressure hole, and at least one portion of the collection compartment is located between the first waste-liquid compartment and the collection compartment pressure hole.
  • the extraction module further includes an absorbing material, the absorbing material is disposed in the first waste-liquid compartment, the first waste-liquid compartment includes a first waste-liquid compartment pressure hole, and at least one portion of the absorbing material is located in a space of the first waste-liquid compartment between the first waste-liquid compartment pressure hole and the first waste-liquid compartment connection hole.
  • the first waste-liquid compartment pressure hole, the second waste-liquid compartment pressure hole and the collection compartment pressure hole are on the same plane.
  • the expansion compartment further includes an expansion compartment inlet, an expansion compartment pressure hole and an expansion compartment spacer, the expansion compartment spacer is located between the expansion compartment inlet and the expansion compartment pressure hole, and the expansion compartment spacer is bent toward the expansion compartment pressure hole.
  • a method for extracting nucleic acid includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module includes a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment. Next, a mixed liquid of sample and alcohol is moved from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid. Then, the mixed liquid is moved to the first waste-liquid compartment. Next, a first detergent is moved from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter. Then, the first detergent is moved to the first waste-liquid compartment.
  • the method for extracting nucleic acid further includes the following steps. First, a second detergent is moved from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter. Then, the second detergent is moved to the second waste-liquid compartment. Next, an eluent is moved from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment. Then, a positive pressure is provided via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and a negative pressure is provided via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment.
  • the reaction compartment and the waste-liquid compartments are incorporated in one single extraction module, and the size and cost of the extraction cassette are reduced.
  • the waste-liquid can be controlled to be moved to the first waste-liquid compartment and the second waste-liquid compartment, and the eluent with the nucleic acid is controlled to be moved to the collection compartment.
  • the waste-liquid is prevented from polluting the reaction compartment and the collection compartment.
  • Fig. 1A is an assembled view of an extraction cassette C of an embodiment of the present invention.
  • Fig. 1B is an exploded view of the extraction cassette C of the embodiment of the present invention.
  • the extraction cassette C includes an extraction module 1, a liquid receiving module 2, a sampling module 3 and a connection module 4.
  • the extraction cassette C is adapted to be disposed into an analyzer.
  • the analyzer includes a first pressure supplying module, an analyzing module and a second pressure supplying module.
  • the first pressure supplying module and the second pressure supplying module provide pressure toward the extraction cassette C to control the liquid movement inside the extraction cassette C.
  • the analyzing module heats and cools the extraction cassette C, and analyzes the sample inside extraction cassette C.
  • the liquid receiving module 2 communicates with the extraction module 1 via the connection module 4.
  • Fig. 2 shows the details of the extraction module 1.
  • the extraction module 1 includes an extraction module body 19, an expansion compartment 18, a reaction compartment 11, a filter 111, a collection compartment 12, a first waste-liquid compartment 13 and a second waste-liquid compartment 14.
  • the expansion compartment 18 is formed on the extraction module body 19.
  • the reaction compartment 11 is formed on the extraction module body 19.
  • the reaction compartment 11 includes a reaction compartment inlet 114, a reaction compartment outlet 112 and a reaction compartment notch 115.
  • the expansion compartment 18 is connected to the reaction compartment notch 115.
  • the reaction compartment notch 115 is located between the reaction compartment inlet 114 and the reaction compartment outlet 112.
  • the filter 111 is disposed in the reaction compartment 11 and corresponding to the reaction compartment outlet 112.
  • the collection compartment 12 is formed on the extraction module body 19 and communicates with the reaction compartment outlet 112.
  • the first waste-liquid compartment 13 is formed on the extraction module body 19.
  • the first waste-liquid compartment 13 communicates with the reaction compartment outlet 112.
  • the second waste-liquid compartment 14 is formed on the extraction module body 19, wherein the second waste-liquid compartment 14 communicates with the reaction compartment outlet 112.
  • the expansion compartment 18 further includes an expansion compartment inlet 181, an expansion compartment pressure hole 182 and an expansion compartment spacer 183.
  • the expansion compartment spacer 183 is located between the expansion compartment inlet 181 and the expansion compartment pressure hole 182.
  • the expansion compartment spacer 183 is bent toward the expansion compartment pressure hole 182.
  • the expansion compartment spacer 183 prevents the liquid entering the expansion compartment 18 via the expansion compartment inlet 181 from polluting the expansion compartment pressure hole 182.
  • the reaction compartment 11 includes a cone-shaped portion 113.
  • the filter 111 is disposed on the cone-shaped portion 113.
  • the reaction compartment outlet 112 is formed on one end of the cone-shaped portion 113.
  • the extraction module 1 further includes a first path 15 and a second path 16.
  • the first path 15 connects the reaction compartment outlet 112 to the first waste-liquid compartment 13 and the second waste-liquid compartment 14.
  • the second path 16 connects the reaction compartment outlet 112 to the collection compartment 12.
  • the first path 15 intersects the second path 16 at the reaction compartment outlet 112.
  • a stopper wall 161 is formed in the second path 16, and the stopper wall 161 is formed on one end of the second path 16 and is adjacent to the reaction compartment outlet 112.
  • the extraction module 1 further includes a third path 151 and a fourth path 152.
  • the third path 151 connects the first path 15 to the first waste-liquid compartment 13.
  • the fourth path 152 connects the first path 15 to the second waste-liquid compartment 14.
  • the first waste-liquid compartment 13 includes a first waste-liquid compartment connection hole 131.
  • the second waste-liquid compartment 14 includes a second waste-liquid compartment connection hole 141.
  • the third path 151 connects the first path 15 to the first waste-liquid compartment connection hole 131.
  • the fourth path 152 connects the first path 15 to the second waste-liquid compartment connection hole 141. At least one portion of the second waste-liquid compartment 14 is located between the first waste-liquid compartment connection hole 131 and the second waste-liquid compartment connection hole 141. In one embodiment, at least one portion of the fourth path 152 extends in a first direction Z, and the first direction Z is away from the first waste-liquid compartment 13. The disclosure is not meant to restrict the present invention.
  • the second waste-liquid compartment 14 includes a second waste-liquid compartment pressure hole 142. At least one portion of the waste-liquid compartment 14 is located between the first waste-liquid compartment 13 and the second waste-liquid compartment pressure hole 142. In one embodiment, at least one portion of the second path 16 extends in the first direction Z. The disclosure is not meant to restrict the present invention.
  • the collection compartment 12 includes a collection compartment pressure hole 121. At least one portion of the collection compartment 12 is located between the first waste-liquid compartment 13 and the collection compartment pressure hole 121.
  • the extraction module 1 further includes an absorbing material 17.
  • the absorbing material 17 is disposed in the first waste-liquid compartment 13.
  • the first waste-liquid compartment 13 includes a first waste-liquid compartment pressure hole 132. At least one portion of the absorbing material 17 is located in a space of the first waste-liquid compartment 13 between the first waste-liquid compartment pressure hole 132 and the first waste-liquid compartment connection hole 131.
  • the absorbing material 17 can be sponge.
  • the first waste-liquid compartment pressure hole 132, the second waste-liquid compartment pressure hole 142 and the collection compartment pressure hole 121 are on the same plane.
  • Figs. 3A ⁇ 3J show the operation of the extraction module 1 of the embodiment of the present invention.
  • a mixed liquid 286 of sample and alcohol is moved from the liquid receiving module 2 to the expansion compartment 18 and the reaction compartment 11 via the expansion compartment inlet 181, and the filter 111 captures the nucleic acid from the mixed liquid 286.
  • the mixed liquid 286 is moved to the first waste-liquid compartment 13 by a negative pressure ( ⁇ -10kpa) provided via the first waste-liquid compartment pressure hole 132.
  • a negative pressure ⁇ -10kpa
  • a first detergent 282 is progressively moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted.
  • the first detergent 282 is moved to the first waste-liquid compartment 13 by the negative pressure ( ⁇ -10kpa) provided via the first waste-liquid compartment pressure hole 132.
  • a portion of second detergent 283 is moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted.
  • Fig. 3C a first detergent 282 is progressively moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted.
  • the second detergent 283 is moved to the first waste-liquid compartment 13 by the negative pressure ( ⁇ -10kpa) provided via the first waste-liquid compartment pressure hole 132. Then, with reference to Fig. 3G , the other portion of second detergent 283 is moved from the liquid receiving module 2 to the filter 111 via the expansion compartment inlet 181, expansion compartment 18 and the reaction compartment 11, and the salts concentration and the PH value on the filter 111 is adjusted. Next, with reference to Fig. 3H , the second detergent 283 is moved to the second waste-liquid compartment 14 by the negative pressure ( ⁇ -10kpa) provided via the second waste-liquid compartment pressure hole 142. Then, with reference to Fig.
  • an eluent 284 is moved from the liquid receiving module 2 to the reaction compartment 11 via the reaction compartment inlet 114, wherein the eluent 284 is resting in the reaction compartment 11 for three minutes, and the nucleic acid is released from the filter 111 to the eluent 284.
  • a big positive pressure 45kpa
  • a little positive pressure is provided via the second waste-liquid compartment pressure hole 142 to prevent the eluent 284 with the nucleic acid from entering the first path 15.
  • the big positive pressure (45kpa) is provided via the liquid receiving module 2 through the reaction compartment inlet 114.
  • the first detergent 282 can be progressively moved from the liquid receiving module 2 to the filter 111, or totally moved from the liquid receiving module 2 to the filter 111 in single one movement, so does the second detergent 283.
  • the disclosure is not meant to restrict the present invention.
  • the first detergent 282 can neutralize PH value
  • the second detergent 283 can remove protein and organic impurities.
  • the expansion compartment 18 prevents the mixed liquid 286, the first detergent 282 and the second detergent 283 from contacting the reaction compartment inlet 114 to prevent the reaction compartment inlet 114 from being polluted.
  • the stopper wall 161 prevents the mixed liquid 286, the first detergent 282 and the second detergent 283 from entering the collection compartment 12.
  • the second path 16 has a bent portion 162.
  • the bent portion 162 makes the eluent 284 with the nucleic acid to sufficiently enter the collection compartment 12.
  • the collection compartment 12 further includes a collection compartment inclined surface 122 and a collection compartment outlet 123.
  • the collection compartment inclined surface 122 connects the collection compartment outlet 123.
  • the collection compartment inclined surface 122 makes the eluent 284 with the nucleic acid to totally leave the collection compartment 12 via the collection compartment outlet 123 (to the sampling module 3, with reference to Fig. 1B ).
  • the bent portion 162 is bent toward the collection compartment inclined surface 122.
  • the second detergent 283 finally entering the second waste-liquid compartment 14 is the cleanest waste-liquid.
  • the little positive pressure is provided via the second waste-liquid compartment pressure hole 142 to prevent the eluent 284 with the nucleic acid from entering the first path 15. Because the second detergent 283 in the second waste-liquid compartment 14 is the cleanest waste-liquid, the pollution caused by the air inside the second waste-liquid compartment 14 to the eluent 284 can be reduced.
  • the waste-liquid in the first waste-liquid compartment 13 contacts the absorbing material 17.
  • the absorbing material 17 removes the bubbles of the waste-liquid, and the waste-liquid is prevented from overflowing out of the first waste-liquid compartment pressure hole 132.
  • the filter 111 can be silicon filter or other filters.
  • the extraction module can be integrally formed.
  • a method for extracting nucleic acid includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the extraction module includes a reaction compartment, a filter, a collection compartment, a first waste-liquid compartment and a second waste-liquid compartment (S11). Next, a mixed liquid of sample and alcohol is moved from the liquid receiving module to the extraction module, and the filter captures nucleic acid from the mixed liquid (S12). Then, the mixed liquid is moved to the first waste-liquid compartment (S13).
  • a first detergent is moved from the liquid receiving module to the extraction module, wherein the first detergent passes through the reaction compartment and the filter (S14). Then, the first detergent is moved to the first waste-liquid compartment (S15).
  • a second detergent is moved from the liquid receiving module to the extraction module, wherein the second detergent passes through the reaction compartment and the filter (S16). Then, the second detergent is moved to the second waste-liquid compartment (S17).
  • an eluent is moved from the liquid receiving module to the extraction module, wherein the eluent is resting in the reaction compartment (S18).
  • a positive pressure is provided via a second waste-liquid compartment pressure hole of the second waste-liquid compartment, and a negative pressure is provided via a collection compartment pressure hole of the collection compartment, wherein the eluent with the nucleic acid is moved to the collection compartment (S19).
  • the reaction compartment and the waste-liquid compartments are incorporated in one single extraction module, and the size and cost of the extraction cassette are reduced.
  • the waste-liquid can be controlled to be moved to the first waste-liquid compartment and the second waste-liquid compartment, and the eluent with the nucleic acid is controlled to be moved to the collection compartment.
  • the waste-liquid is prevented from polluting the reaction compartment and the collection compartment.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP18215536.6A 2017-12-26 2018-12-21 Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür Active EP3505253B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762610444P 2017-12-26 2017-12-26
CN201811535018.6A CN110004141A (zh) 2017-12-26 2018-12-14 核酸萃取方法及其萃取卡匣

Publications (2)

Publication Number Publication Date
EP3505253A1 true EP3505253A1 (de) 2019-07-03
EP3505253B1 EP3505253B1 (de) 2021-07-28

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Application Number Title Priority Date Filing Date
EP18215536.6A Active EP3505253B1 (de) 2017-12-26 2018-12-21 Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114062094A (zh) * 2020-07-31 2022-02-18 深圳市帝迈生物技术有限公司 一种溶血装置、糖化液路系统及多功能一体机

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080153078A1 (en) * 2006-06-15 2008-06-26 Braman Jeffrey C System for isolating biomolecules from a sample
GB2516666A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd Fluidic cartridge for nucleic acid amplification and detection

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080153078A1 (en) * 2006-06-15 2008-06-26 Braman Jeffrey C System for isolating biomolecules from a sample
GB2516666A (en) * 2013-07-29 2015-02-04 Atlas Genetics Ltd Fluidic cartridge for nucleic acid amplification and detection

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114062094A (zh) * 2020-07-31 2022-02-18 深圳市帝迈生物技术有限公司 一种溶血装置、糖化液路系统及多功能一体机

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