EP3505252B1 - Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür - Google Patents

Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür Download PDF

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Publication number
EP3505252B1
EP3505252B1 EP18215506.9A EP18215506A EP3505252B1 EP 3505252 B1 EP3505252 B1 EP 3505252B1 EP 18215506 A EP18215506 A EP 18215506A EP 3505252 B1 EP3505252 B1 EP 3505252B1
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EP
European Patent Office
Prior art keywords
compartment
sample
receiving module
detergent
mixing
Prior art date
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Application number
EP18215506.9A
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English (en)
French (fr)
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EP3505252A3 (de
EP3505252A2 (de
Inventor
Wei-Yu Chung
Song-Bin Huang
Shing-Lun Liu
Yu-Kai Kao
Yi-Chen Li
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Delta Electronics Inc
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Delta Electronics Inc
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Priority claimed from CN201811532891.XA external-priority patent/CN109966776B/zh
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/52—Containers specially adapted for storing or dispensing a reagent
    • B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/028—Graduation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs

Definitions

  • the present invention relates to an extraction cassette with a liquid receiving module, and a method for extracting nucleic acid from a sample.
  • the sample compartment typically has a pressure supply opening connected to a pressure source.
  • the movement of the liquid inside the sample compartment can be controlled by the pressure supplied via the pressure supply opening.
  • the sample often contains viruses, bacteria or other contaminants, and the contaminants can travel to the analyzer or the outer environment via the pressure supply opening. Such contaminants not only cause inaccurate test results, but also endanger the health of the operator.
  • US 2017/015993 A1 discloses an extraction cassette for extracting nucleic acid from a mixed liquid including a sample according to the preamble of claim 1.
  • the extraction cassette comprises a liquid receiving module with several compartments for receiving the sample and liquids and a connection module for establishing certain liquid flow pathways for conducting the sample to be tested and liquids.
  • the sample is loaded to the sample reservoir provided in a lower module.
  • For extracting the nucleic acid external air is induced into the lower module via air holes on a lower membrane by an air pump. Channels in the lower module communicate with corresponding channels in an upper module, and thus the liquids including the sample material are transferred via the channels provided in the upper surface of the upper module.
  • the sample must be loaded to the sample reservoir in the lower module while the lower module and the upper module are not yet assembled together. There is not provided any sealant that seals a filling hole of the sample reservoir. Rather, the sample reservoir is open at its upper end. Communication between the liquid / sample receiving chambers in the lower module is accomplished only via the channels in the upper module. No connection holes and fluid pathways are provided in the lower module.
  • US 2015/024436 A1 an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in short time.
  • the device can be used for extracting nucleic acid and comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.
  • the analyte preparation module has a different configuration.
  • the sample feeding hole of the sample compartment is sealed by the sealing member.
  • the mixed liquid inside the sample compartment is moved to the first mixing compartment by the pressure applied to the alcohol compartment. Therefore, the virus, bacteria and other contaminants of the sample in the sample compartment is prevented from leaking from the liquid receiving module. Even if the contaminants of the sample enter the alcohol compartment via the first sample compartment connection hole (above the sample compartment) and the first path, the contaminants are disinfected by the alcohol compartment and are unable to cause pollution.
  • the extraction cassette of the embodiment of the present invention can restrict the area polluted by the sample to the environment inside or around the analyzer, improving the accuracy of the analysis result and safeguarding the health of the operator.
  • Fig. 1A is an assembled view of an extraction cassette C of an embodiment of the present invention.
  • Fig. 1B is an exploded view of the extraction cassette C of the embodiment of the present invention.
  • the extraction cassette C includes an extraction module 1, a liquid receiving module 2, a sampling module 3 and a connection module 4.
  • the extraction cassette C is adapted to be disposed into an analyzer.
  • the analyzer includes a first pressure supplying module, an analyzing module and a second pressure supplying module.
  • the first pressure supplying module and the second pressure supplying module provide pressure toward the extraction cassette C to control the liquid movement inside the extraction cassette C.
  • the analyzing module heats and cools the extraction cassette C, and analyzes the sample inside extraction cassette C.
  • the liquid receiving module 2 communicates with the extraction module 1 via the connection module 4.
  • the compartments of the liquid receiving module 2 communicate with one another via the connection module 4.
  • Fig. 2A is a detail of the liquid receiving module 2.
  • the liquid receiving module 2 includes a receiving module body 29, a sample compartment 21, a sealing member 211, an alcohol compartment 22 and a first path 221.
  • the receiving module body 29 includes a first side 291, a second side 292 and a sample feeding hole 212.
  • the first side 291 is opposite the second side 292.
  • the sample feeding hole 212 is formed on the first side 291.
  • the sample compartment 21 is formed on the receiving module body 29.
  • the sample compartment 21 communicates with the sample feeding hole 212.
  • the sample compartment 21 includes a first sample compartment connection hole 213 and a second sample compartment connection hole 214.
  • the first sample compartment connection hole 213 nears the first side 291 relative to the second side 292.
  • the sealing member 211 is adapted to seal the sample feeding hole 212.
  • the alcohol compartment 22 is formed on the receiving module body 29.
  • the alcohol compartment 22 communicates with the first sample compartment connection hole 213 of the first sample compartment 21 via the first path 221. In one embodiment, wherein at least one portion of the first path 221 is located between the alcohol compartment 22 and the second side 292.
  • the alcohol compartment 22 includes an alcohol compartment connection hole 222.
  • the alcohol compartment connection hole 222 nears the second side 292 relative to the first side 291.
  • the first path 221 connects the alcohol compartment connection hole 222 to the first sample compartment connection hole 213.
  • the liquid receiving module 2 further includes a first mixing compartment 23 and a second path 231.
  • the first mixing compartment 23 is formed on the receiving module body 29.
  • the second sample compartment connection hole 214 nears the second side 292 relative to the first side 291.
  • the second path 231 connects the second sample compartment connection hole 214 to the first mixing compartment 23.
  • the liquid receiving module 2 further includes a second mixing compartment 24, a third path 241 and a fourth path 242.
  • the second mixing compartment 24 is formed on the receiving module body 29.
  • the third path 241 connects the first mixing compartment 23 to the second mixing compartment 24.
  • the fourth path 242 connects the second mixing compartment 24 to the extraction module 1.
  • the third path 241 connects the first mixing compartment 23 to the bottom of the second mixing compartment 24.
  • the liquid receiving module 2 further includes a first detergent compartment 25, a second detergent compartment 26, a fifth path 251 and a sixth path 261.
  • the first detergent compartment 25 and the second detergent compartment 26 are formed on the receiving module body 29.
  • the fifth path 251 connects the first detergent compartment 25 to the first mixing compartment 23.
  • the sixth path 261 connects the second detergent compartment 26 to the first mixing compartment 23.
  • the liquid receiving module further includes an eluent compartment 27 and a seventh path 271.
  • the eluent compartment 27 is formed on the receiving module body 29, and the seventh path 271 connects the eluent compartment 27 to the extraction module 1.
  • Figs. 3A ⁇ 3I show the operation of the liquid receiving module 2 of the embodiment of the present invention.
  • liquid receiving module 2 receives four liquids, which are the alcohol 281 (in the alcohol compartment 22), the first detergent 282 (in the first detergent compartment 25), the second detergent 283 (in the second detergent compartment 26) and the eluent 284 (in the eluent compartment 27).
  • the sample 285 is filled into the sample compartment 21.
  • the temperature of the sample 285 in the sample compartment 21 is raised to 60 degrees Celsius (60 °C).
  • the alcohol 281 from the alcohol compartment 22 to the sample compartment 21, wherein the sample 285 and the alcohol 281 are mixed into a mixed liquid 286 in the sample compartment 21.
  • the mixed liquid 286 with the sample 285 and the alcohol 281 is moved from the sample compartment 21 to the first mixing compartment 23 ( Fig. 3D ).
  • the mixed liquid 286 is moved between the first mixing compartment 23 and the second mixing compartment 24 repeatedly to mix the sample 285 and the alcohol 281 ( Fig. 3E ).
  • the mixed liquid 286 with the sample 285 and the alcohol 281 is moved into the extraction module 1 and the nucleic acid is captured from the mixed liquid 286 by the extraction module 1. Then, the mixed liquid 286 is moved into a first waste-liquid compartment of the extraction module 1.
  • the first detergent 282 is progressively moved from the first detergent compartment 25 to the first mixing compartment 23 and the second mixing compartment 24 to clean the first mixing compartment 23 and the second mixing compartment 24. Then, the first detergent 282 is moved to the extraction module 1 to clean the extraction module 1. Next, the first detergent 282 is moved to the first waste-liquid compartment of the extraction module 1.
  • the second detergent 283 is progressively moved from the second detergent compartment 26 to the first mixing compartment 23 and the second mixing compartment 24 to clean the first mixing compartment 23 and the second mixing compartment 24. Then, the second detergent 283 is moved to the extraction module 1 to clean the extraction module 1. Next, at least one portion of the second detergent 283 is moved to a second waste-liquid compartment of the extraction module 1.
  • the eluent 284 is from the eluent compartment 27 to the extraction module 1 to take out the nucleic acid from the extraction module 1.
  • the eluent 284 with the nucleic acid is then moved to the sampling module 3 to be quantitatively sampled.
  • the sample feeding hole 212 of the sample compartment 21 is sealed by the sealing member 211.
  • the mixed liquid 286 inside the sample compartment 21 is moved to the first mixing compartment 23 by the pressure applied to the alcohol compartment 22. Therefore, the virus, bacteria and other contaminants of the sample 285 in the sample compartment 21 is prevented from leaking from the liquid receiving module. Even if the contaminants of the sample 285 enter the alcohol compartment 22 via the first sample compartment connection hole (above the sample compartment 21) and the first path 221, the contaminants are disinfected by the alcohol compartment 22 and are unable to cause pollution.
  • the extraction cassette of the embodiment of the present invention can restrict the area polluted by the sample 285 to the environment inside or around the analyzer, improving the accuracy of the analysis result and safeguarding the health of the operator.
  • the mixed liquid 286 can be moved back and forth between the first mixing chamber 23 and the second mixing chamber 24 via the third path 241.
  • the mixed liquid 286 is thoroughly mixed, whereby the effect of sufficiently mixing the mixed liquid 286 can be achieved with a minimum space.
  • the fourth path 242 has a fourth path outlet 242A that is higher than the top of the first mixing chamber 23 and the second mixing chamber 24.
  • the fourth path outlet 242A is higher than the liquid level of the mixed liquid 286.
  • the liquid receiving module 2 can be integrally formed except the sealing member 211.
  • Figs. 4A ⁇ 4C show a method for extracting nucleic acid of an embodiment of the present invention.
  • the method for extracting nucleic acid of the embodiment of the present invention includes the following steps. First, an extraction cassette is provided, wherein the extraction cassette includes a liquid receiving module and an extraction module, the extraction module communicates with the liquid receiving module, the liquid receiving module includes a sample compartment, an alcohol compartment, a first mixing compartment, a second mixing compartment, a first detergent compartment, a second detergent compartment and an eluent compartment (S11).
  • an alcohol is filled into the alcohol compartment, a first detergent is filled into the first detergent compartment, a second detergent is filled into the second detergent compartment, and an eluent is filled into the eluent compartment (S12).
  • a sample is filled into the sample compartment (S13). Then, the sample inside the sample compartment is heated (S14).
  • the method further includes the following steps.
  • the alcohol is moved from the alcohol compartment to the sample compartment, wherein the sample and the alcohol are mixed into a mixed liquid (S15).
  • the mixed liquid is moved into the first mixing compartment, and then the mixed liquid is moved between the first mixing compartment and the second mixing compartment repeatedly to mix the sample and the alcohol (S16).
  • the mixed liquid is moved into the extraction module and the nucleic acid is captured from the mixed liquid by the extraction module (S17).
  • the mixed liquid is moved into a first waste-liquid compartment of the extraction module (S18).
  • the method further includes the following steps.
  • the first detergent is progressively moved from the first detergent compartment to the first mixing compartment and the second mixing compartment to clean the first mixing compartment and the second mixing compartment (S21).
  • the first detergent is moved to the extraction module to clean the extraction module (S22).
  • the first detergent is moved to the first waste-liquid compartment of the extraction module (S23).
  • the second detergent is progressively moved from the second detergent compartment to the first mixing compartment and the second mixing compartment to clean the first mixing compartment and the second mixing compartment (S24).
  • the second detergent is moved to the extraction module to clean the extraction module (S25).
  • at least one portion of the second detergent is moved to a second waste-liquid compartment of the extraction module (S26).
  • the eluent is moved from the eluent compartment to the extraction module to take out the nucleic acid from the extraction module (S27).
  • the applicant used an oral cell preservation solution containing a high concentration of the nucleic acid to be tested (Mh plasmid: 106 copies/mL, CytB plasmid) as a positive sample, and an oral cell preservation solution containing no nucleic acid to be tested as a negative sample, and perform interleaved tests of positive and negative samples. It is desired to confirm that the negative sample test result after the positive sample test result has no nucleic acid detected. It has been experimentally verified that the extraction cassette of the embodiment of the present invention can effectively prevent cross-contamination.
  • Test 1 High concentration positive and negative samples are alternatively tested four times (test sequence: positive ⁇ negative ⁇ positive ⁇ negative) Result: no nucleic acid detected in negative samples Samples positive negative positive negative Mh plasmid1 Cq 25.11 N/A 26.48 N/A CytB plasmid Cq 24.54 N/A 28.1 N/A Test 2: Negative sample test after five high consecution positive sample tests (test sequence: positive ⁇ positive ⁇ positive ⁇ positive ⁇ negative) Result: no nucleic acid detected in negative samples Samples positive positive positive positive negative Mh plasmid1 Cq 28.33 27.81 27.18 24.82 26.44 N/A CytB plasmid Cq 26.89 23.51 25.85 24.68 28.58 N/A

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Claims (12)

  1. Extraktionskassette (C) zum Extrahieren von Nukleinsäure aus einer gemischten Flüssigkeit (286), die eine Probe (285) enthält, umfassend:
    ein Flüssigkeitsaufnahmemodul (2) zum Aufnehmen der Probe (285) und des Alkohols (281) und zum Mischen der Probe (285) mit dem Alkohol (281), um die gemischte Flüssigkeit (286) zu erzeugen; und
    ein Extraktionsmodul (1) zum Gewinnen der Nukleinsäure aus der gemischten Flüssigkeit (286); wobei
    das Flüssigkeitsaufnahmemodul (2) mit dem Extraktionsmodul (1) in Verbindung steht, und
    das Flüssigkeitsaufnahmemodul (2) einen Aufnahmemodulkörper (29) mit einer ersten Seite (291), einer der ersten Seite (291) gegenüberliegenden zweiten Seite (292) und einer auf der ersten Seite (291) ausgebildeten Probenzuführöffnung (212) umfasst; wobei
    der Aufnahmemodulkörper (29) umfasst:
    einen Probenraum (21), der auf dem Aufnahmemodulkörper (29) ausgebildet und konfiguriert ist, um die Probe (285) aufzunehmen, wobei der Probenraum (21) mit dem Probenzuführöffnung (212) in Verbindung steht, und
    einen Alkoholraum (22), der auf dem Aufnahmemodulkörper (29) ausgebildet und so konfiguriert ist, dass dieser den Alkohol (281) aufnimmt, wobei
    der Alkoholraum (22) mit dem ersten Probenraum (21) über einen ersten Pfad (221) in Verbindung steht;
    dadurch gekennzeichnet, dass
    das Flüssigkeitsaufnahmemodul (2) ferner ein Dichtungselement (211) umfasst, das zum Abdichten der Probenzuführöffnung (212) angepasst ist, wobei
    der Probenraum (21) eine erste Probenraum-Verbindungsöffnung (213) und eine zweite Probenraum-Verbindungsöffnung (214) zur Verbindung mit dem Extraktionsmodul (1) aufweist,
    die erste Probenraum-Verbindungsöffnung (213) sich der ersten Seite (291) relativ zur zweiten Seite (292) nähert,
    der Alkoholraum (22) eine Alkoholraum-Verbindungsöffnung (222) umfasst, die sich der zweiten Seite (292) in Bezug auf die erste Seite (291) nähert,
    der erste Pfad (221) die Alkoholraum-Verbindungsöffnung (222) mit der ersten Probenraum-Verbindungsöffnung (213) verbindet, um den Alkohol (281) aus dem Alkoholraum (22) in den Probenraum (21) zu überführen, und
    mindestens ein Teil des ersten Pfades (221) sich zwischen dem Alkoholraum (22) und der zweiten Seite (292) befindet.
  2. Extraktionskassette nach Anspruch 1, wobei das Flüssigkeitsaufnahmemodul (29) ferner einen ersten Mischraum (23) und einen zweiten Pfad (231) aufweist, der erste Mischraum (23) auf dem Aufnahmemodulkörper (29) ausgebildet ist, die zweite Probenraum-Verbindungsöffnung (214) sich der zweiten Seite (292) relativ zur ersten Seite (291) nähert und der zweite Pfad (231) die zweite Probenraum-Verbindungsöffnung (214) mit dem ersten Mischraum (23) verbindet.
  3. Extraktionskassette nach Anspruch 2, wobei das Flüssigkeitsaufnahmemodul (29) ferner einen zweiten Mischraum (24), einen dritten Pfad (241) und einen vierten Pfad (242) aufweist, der zweite Mischraum (24) an dem Aufnahmemodulkörper (29) ausgebildet ist, der dritte Pfad (241) den ersten Mischraum (23) mit dem zweiten Mischraum (24) verbindet, der vierte Pfad (242) den zweiten Mischraum (24) mit dem Extraktionsmodul (1) verbindet, der vierte Pfad (242) einen vierten Pfadauslass (242A) umfasst, der sich der zweiten Seite (292) relativ zu dem ersten Mischraum (23) und dem zweiten Mischraum (24) nähert.
  4. Extraktionskassette nach Anspruch 3 oder 4, wobei das Flüssigkeitsaufnahmemodul (2) ferner einen ersten Detergens-Raum (25), einen zweiten Detergens-Raum (26), einen fünften Pfad (251) und einen sechsten Pfad (261) umfasst, der erste Detergens-Raum (25) und der zweite Detergens-Raum (26) an dem Aufnahmemodulkörper (29) ausgebildet sind, der fünfte Pfad (251) den ersten Detergens-Raum (25) mit dem ersten Mischraum (23) verbindet und der sechste Pfad (261) den zweiten Detergens-Raum (26) mit dem ersten Mischraum (23) verbindet.
  5. Extraktionskassette nach einem der vorhergehenden Ansprüche, wobei das Flüssigkeitsaufnahmemodul (2) ferner einen Eluenten-Raum (27) und einen siebten Pfad (271) aufweist, der Eluenten-Raum (27) an dem Aufnahmemodulkörper (29) ausgebildet ist und der siebte Pfad (271) den Eluenten-Raum (27) mit dem Extraktionsmodul (1) verbindet.
  6. Verfahren zum Extrahieren von Nukleinsäure aus einer Probe (285) unter Verwendung einer Extraktionskassette (C) nach einem der Ansprüche 1 bis 5, umfassend die folgenden Schritte:
    Einfüllen eines Alkohols (281) in den Alkoholraum (22) des Aufnahmemodulkörper (29);
    Einfüllen einer Probe (285) in den Probenraum (21) des Aufnahmemodulkörper (29);
    Erwärmen der Probe (285) innerhalb des Probenraums (21) und Abdichten der Probenzuführöffnung (212) des Probenraums (21) des Aufnahmemodulkörper (29) mittels eines Dichtungselements (211);
    Bewegen des Alkohols (281) aus dem Alkoholraum (22) in den Probenraum (21), wobei die Probe und der Alkohol zu einer gemischten Flüssigkeit (286) vermischt werden; und
    Einbringen der gemischten Flüssigkeit (286) in das Extraktionsmodul (1) und Gewinnen der Nukleinsäure aus der gemischten Flüssigkeit durch das Extraktionsmodul (1).
  7. Verfahren nach Anspruch 6, weiter umfassend:
    Einfüllen eines ersten Detergens (282) in einen ersten Detergens-Raum (25) des Aufnahmemodulkörpers (29),
    Einfüllen eines zweiten Reinigungsmittels (283) in einen zweiten Detergens-Raum (26) des Aufnahmemodulkörper (29), und
    Einfüllen eines Eluenten (284) in einen Eluenten-Raum (27) des Aufnahmemodulkörpers (29).
  8. Verfahren nach Anspruch 7, das ferner die folgenden Schritte umfasst:
    Bewegen der gemischten Flüssigkeit (286) in einen ersten Mischraum (23) des Aufnahmemodulkörper (29); und
    wiederholtes Bewegen der gemischten Flüssigkeit (286) zwischen dem ersten Mischraum (23) und einem zweiten Mischraum (24) des Aufnahmemodulkörpers (29), um die Probe (285) und den Alkohol (281) zu mischen.
  9. Verfahren nach Anspruch 8, das ferner die folgenden Schritte umfasst:
    Bewegen der gemischten Flüssigkeit (286) in einen ersten Abfall-Flüssigkeits-Raum des Extraktionsmoduls (1).
  10. Verfahren nach Anspruch 8 oder 9, das ferner die folgenden Schritte umfasst:
    schrittweises Bewegen des ersten Detergens (282) aus dem ersten Detergens-Raum (25) in den ersten Mischraum (23) und den zweiten Mischraum (24), um den ersten Mischraum und den zweiten Mischraum zu reinigen;
    Bewegen des ersten Detergens (282) zu dem Extraktionsmodul (1), um das Extraktionsmodul zu reinigen; und
    Bewegen des ersten Detergens (282) zu dem ersten Abfall-Flüssigkeits-Raum des Extraktionsmoduls (1).
  11. Verfahren nach einem der Ansprüche 8 bis 10, das ferner die folgenden Schritte umfasst:
    schrittweises Bewegen des zweiten Detergens (283) aus dem zweiten Detergens-Raum (26) in den ersten Mischraum (23) und den zweiten Mischraum (24), um den ersten Mischraum und den zweiten Mischraum zu reinigen;
    Bewegen des zweiten Detergens (283) zu dem Extraktionsmodul (1), um das Extraktionsmodul zu reinigen; und
    Bewegen mindestens eines Teils des zweiten Detergens (283) in einen zweiten Abfall-Flüssigkeits-Raum des Extraktionsmoduls (1).
  12. Verfahren nach Anspruch 11, das ferner einen Schritt umfasst
    Bewegen des Eluenten (284) aus dem Eluenten-Raum (27) zu dem Extraktionsmodul (1), um die Nukleinsäure aus dem Extraktionsmodul (1) zu gewinnen.
EP18215506.9A 2017-12-26 2018-12-21 Verfahren zur extraktion von nukleinsäure und extraktionskassette dafür Active EP3505252B1 (de)

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Application Number Priority Date Filing Date Title
US201762610444P 2017-12-26 2017-12-26
CN201811532891.XA CN109966776B (zh) 2017-12-26 2018-12-14 核酸萃取方法及其萃取卡匣

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EP3505252A3 EP3505252A3 (de) 2019-07-17
EP3505252B1 true EP3505252B1 (de) 2021-02-24

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EP3505252A2 (de) 2019-07-03

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