EP3500684A4 - OPTICAL-BASED NANOPORE SEQUENCING USING EXTINGUISHING AGENTS - Google Patents
OPTICAL-BASED NANOPORE SEQUENCING USING EXTINGUISHING AGENTS Download PDFInfo
- Publication number
- EP3500684A4 EP3500684A4 EP17841831.5A EP17841831A EP3500684A4 EP 3500684 A4 EP3500684 A4 EP 3500684A4 EP 17841831 A EP17841831 A EP 17841831A EP 3500684 A4 EP3500684 A4 EP 3500684A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- optically
- nanopore sequencing
- quenching agents
- based nanopore
- quenching
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662377409P | 2016-08-19 | 2016-08-19 | |
PCT/US2017/043772 WO2018034807A1 (en) | 2016-08-19 | 2017-07-25 | Optically-based nanopore sequencing using quenching agents |
Publications (2)
Publication Number | Publication Date |
---|---|
EP3500684A1 EP3500684A1 (en) | 2019-06-26 |
EP3500684A4 true EP3500684A4 (en) | 2020-02-26 |
Family
ID=61196974
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP17841831.5A Withdrawn EP3500684A4 (en) | 2016-08-19 | 2017-07-25 | OPTICAL-BASED NANOPORE SEQUENCING USING EXTINGUISHING AGENTS |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP3500684A4 (ja) |
JP (1) | JP2019528065A (ja) |
CN (1) | CN109804087A (ja) |
AU (1) | AU2017312746A1 (ja) |
CA (1) | CA3031812A1 (ja) |
WO (1) | WO2018034807A1 (ja) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2018034807A1 (en) * | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
WO2021031109A1 (zh) * | 2019-08-20 | 2021-02-25 | 深圳华大智造极创科技有限公司 | 一种基于发光标记物光信号动力学及二次发光信号对多核苷酸进行测序的方法 |
US20220364991A1 (en) * | 2021-03-24 | 2022-11-17 | Northeastern University | Method and System for Decoding Information Stored on a Polymer Sequence |
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US20120270221A1 (en) * | 2006-05-12 | 2012-10-25 | Pronucleotein Biotechnologies, Llc | Methods of sequencing fluorophore-quencher FRET-aptamers |
US20130203050A1 (en) * | 2009-09-30 | 2013-08-08 | Quantapore, Inc. | Hybrid nanopore device with optical detection and methods of using same |
WO2016065339A1 (en) * | 2014-10-24 | 2016-04-28 | Quantapore, Inc. | Efficient optical analysis of polymers using arrays of nanostructures |
US20160122812A1 (en) * | 2014-10-10 | 2016-05-05 | Quantapore, Inc. | Nanopore-based polymer analysis with mutally-quenching fluorescent labels |
WO2017180319A1 (en) * | 2016-04-14 | 2017-10-19 | Quantapore, Inc. | Mixed optical signals in polymer analysis with nanopores |
WO2018034807A1 (en) * | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
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US5405747A (en) | 1991-09-25 | 1995-04-11 | The Regents Of The University Of California Office Of Technology Transfer | Method for rapid base sequencing in DNA and RNA with two base labeling |
US5798042A (en) | 1994-03-07 | 1998-08-25 | Regents Of The University Of California | Microfabricated filter with specially constructed channel walls, and containment well and capsule constructed with such filters |
US6362002B1 (en) | 1995-03-17 | 2002-03-26 | President And Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
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US6267872B1 (en) | 1998-11-06 | 2001-07-31 | The Regents Of The University Of California | Miniature support for thin films containing single channels or nanopores and methods for using same |
US6426231B1 (en) | 1998-11-18 | 2002-07-30 | The Texas A&M University System | Analyte sensing mediated by adapter/carrier molecules |
AU751168B2 (en) | 1999-04-23 | 2002-08-08 | Molecular Probes, Inc. | Xanthene dyes and their application as luminescence quenching compounds |
US20040214221A1 (en) | 1999-05-07 | 2004-10-28 | Klaus Muehlegger | High density labeling of DNA with modified or "chromophore" carrying nucleotides and DNA polymerases used |
US6464842B1 (en) | 1999-06-22 | 2002-10-15 | President And Fellows Of Harvard College | Control of solid state dimensional features |
US6727356B1 (en) | 1999-12-08 | 2004-04-27 | Epoch Pharmaceuticals, Inc. | Fluorescent quenching detection reagents and methods |
WO2001059453A2 (en) | 2000-02-11 | 2001-08-16 | The Texas A & M University System | Biosensor compositions and methods of use |
US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
US7019129B1 (en) | 2000-05-09 | 2006-03-28 | Biosearch Technologies, Inc. | Dark quenchers for donor-acceptor energy transfer |
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EP1504114B1 (en) | 2002-05-10 | 2017-07-12 | The Texas A & M University System | Stochastic sensing through covalent interactions |
JP4694201B2 (ja) | 2002-09-20 | 2011-06-08 | インテグレイテッド ディーエヌエイ テクノロジーズ インコーポレイテッド | アントラキノン消光色素、それらの製造方法及び使用 |
US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
EP1601760A4 (en) | 2003-02-28 | 2009-08-19 | Univ Brown | NANOPORES, METHOD OF THEIR USE, METHOD FOR THE PRODUCTION THEREOF AND METHOD FOR CHARACTERIZING BIOMOLECULES USING THEREOF |
US20050095599A1 (en) * | 2003-10-30 | 2005-05-05 | Pittaro Richard J. | Detection and identification of biopolymers using fluorescence quenching |
US7972858B2 (en) | 2004-08-13 | 2011-07-05 | President And Fellows Of Harvard College | Ultra high-throughput opti-nanopore DNA readout platform |
US7397232B2 (en) | 2005-10-21 | 2008-07-08 | The University Of Akron | Coulter counter having a plurality of channels |
US8883689B2 (en) | 2005-12-06 | 2014-11-11 | Bayer Cropscience Lp | Stabilized herbicidal compositions |
US8003319B2 (en) | 2007-02-02 | 2011-08-23 | International Business Machines Corporation | Systems and methods for controlling position of charged polymer inside nanopore |
EP3543357A1 (en) | 2007-05-08 | 2019-09-25 | Trustees of Boston University | Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof |
GB0713143D0 (en) | 2007-07-06 | 2007-08-15 | Ucl Business Plc | Nucleic acid detection method |
JP2009044967A (ja) * | 2007-08-14 | 2009-03-05 | Sony Corp | 二本鎖核酸の形成に係る情報を得る方法 |
WO2010007537A1 (en) | 2008-07-17 | 2010-01-21 | Koninklijke Philips Electronics N.V. | Nanopore device and a method for nucleic acid analysis |
ES2576114T3 (es) | 2008-09-22 | 2016-07-05 | University Of Washington | Nanoporos MSP y procedimientos relacionados |
KR101797773B1 (ko) | 2009-01-30 | 2017-11-15 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 막횡단 시퀀싱에서 핵산 구축물용 어댑터 |
JP5687683B2 (ja) | 2009-03-26 | 2015-03-18 | トラスティーズ オブ ボストン ユニバーシティ | 2種類の流体間の薄い固相界面上における撮像方法 |
AU2010326349B2 (en) | 2009-12-01 | 2015-10-29 | Oxford Nanopore Technologies Limited | Biochemical analysis instrument |
EP2737084B1 (en) | 2011-07-25 | 2017-10-18 | Oxford Nanopore Technologies Limited | Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores |
US9862997B2 (en) | 2013-05-24 | 2018-01-09 | Quantapore, Inc. | Nanopore-based nucleic acid analysis with mixed FRET detection |
CN104253263A (zh) | 2013-06-28 | 2014-12-31 | 富泰华工业(深圳)有限公司 | 三级性电池 |
-
2017
- 2017-07-25 WO PCT/US2017/043772 patent/WO2018034807A1/en unknown
- 2017-07-25 AU AU2017312746A patent/AU2017312746A1/en not_active Abandoned
- 2017-07-25 JP JP2019509522A patent/JP2019528065A/ja active Pending
- 2017-07-25 CA CA3031812A patent/CA3031812A1/en not_active Abandoned
- 2017-07-25 CN CN201780062870.3A patent/CN109804087A/zh active Pending
- 2017-07-25 EP EP17841831.5A patent/EP3500684A4/en not_active Withdrawn
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120270221A1 (en) * | 2006-05-12 | 2012-10-25 | Pronucleotein Biotechnologies, Llc | Methods of sequencing fluorophore-quencher FRET-aptamers |
US20130203050A1 (en) * | 2009-09-30 | 2013-08-08 | Quantapore, Inc. | Hybrid nanopore device with optical detection and methods of using same |
US20160122812A1 (en) * | 2014-10-10 | 2016-05-05 | Quantapore, Inc. | Nanopore-based polymer analysis with mutally-quenching fluorescent labels |
WO2016065339A1 (en) * | 2014-10-24 | 2016-04-28 | Quantapore, Inc. | Efficient optical analysis of polymers using arrays of nanostructures |
WO2017180319A1 (en) * | 2016-04-14 | 2017-10-19 | Quantapore, Inc. | Mixed optical signals in polymer analysis with nanopores |
WO2018034807A1 (en) * | 2016-08-19 | 2018-02-22 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
Non-Patent Citations (1)
Title |
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See also references of WO2018034807A1 * |
Also Published As
Publication number | Publication date |
---|---|
CA3031812A1 (en) | 2018-02-22 |
CN109804087A (zh) | 2019-05-24 |
JP2019528065A (ja) | 2019-10-10 |
EP3500684A1 (en) | 2019-06-26 |
AU2017312746A1 (en) | 2019-01-31 |
WO2018034807A1 (en) | 2018-02-22 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE INTERNATIONAL PUBLICATION HAS BEEN MADE |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: REQUEST FOR EXAMINATION WAS MADE |
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17P | Request for examination filed |
Effective date: 20190208 |
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AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
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AX | Request for extension of the european patent |
Extension state: BA ME |
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RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: MENCHEN, STEVEN Inventor name: MACEVICZ, STEPHEN C. |
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DAV | Request for validation of the european patent (deleted) | ||
DAX | Request for extension of the european patent (deleted) | ||
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: QUANTAPORE INC. |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20200124 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12Q 1/68 20180101AFI20200120BHEP Ipc: C12Q 1/6869 20180101ALI20200120BHEP |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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17Q | First examination report despatched |
Effective date: 20210401 |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: EXAMINATION IS IN PROGRESS |
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RAP3 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: QUANTAPORE, INC. |
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STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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18D | Application deemed to be withdrawn |
Effective date: 20230201 |