EP3363910A1 - Électrode de surveillance d'analyte continue avec enzyme réticulé - Google Patents

Électrode de surveillance d'analyte continue avec enzyme réticulé Download PDF

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Publication number
EP3363910A1
EP3363910A1 EP17156652.4A EP17156652A EP3363910A1 EP 3363910 A1 EP3363910 A1 EP 3363910A1 EP 17156652 A EP17156652 A EP 17156652A EP 3363910 A1 EP3363910 A1 EP 3363910A1
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EP
European Patent Office
Prior art keywords
electrode
enzyme molecule
conjugate
enzyme
nanoparticle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP17156652.4A
Other languages
German (de)
English (en)
Other versions
EP3363910B1 (fr
EP3363910C0 (fr
Inventor
Angelika FUERST
Daniel KAMMERER
Reinhold Mischler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Roche Diabetes Care GmbH
Original Assignee
F Hoffmann La Roche AG
Roche Diabetes Care GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to EP17156652.4A priority Critical patent/EP3363910B1/fr
Application filed by F Hoffmann La Roche AG, Roche Diabetes Care GmbH filed Critical F Hoffmann La Roche AG
Priority to PCT/EP2018/053917 priority patent/WO2018149981A1/fr
Priority to CN201880003937.0A priority patent/CN109804076A/zh
Priority to JP2019543388A priority patent/JP6801119B2/ja
Priority to RU2019127385A priority patent/RU2731646C1/ru
Priority to CA3035410A priority patent/CA3035410C/fr
Priority to US16/484,900 priority patent/US11535881B2/en
Publication of EP3363910A1 publication Critical patent/EP3363910A1/fr
Application granted granted Critical
Publication of EP3363910B1 publication Critical patent/EP3363910B1/fr
Publication of EP3363910C0 publication Critical patent/EP3363910C0/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54373Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438Electrodes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00Apparatus for enzymology or microbiology
    • C12M1/40Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/005Enzyme electrodes involving specific analytes or enzymes
    • C12Q1/006Enzyme electrodes involving specific analytes or enzymes for glucose
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001Enzyme electrodes
    • C12Q1/005Enzyme electrodes involving specific analytes or enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28Electrolytic cell components
    • G01N27/30Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3278Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction involving nanosized elements, e.g. nanogaps or nanoparticles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/03Oxidoreductases acting on the CH-OH group of donors (1.1) with a oxygen as acceptor (1.1.3)
    • C12Y101/03004Glucose oxidase (1.1.3.4)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/30Hydrogen technology
    • Y02E60/50Fuel cells

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Emergency Medicine (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Nanotechnology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
EP17156652.4A 2017-02-17 2017-02-17 Électrode de surveillance d'analyte continue avec enzyme réticulé Active EP3363910B1 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP17156652.4A EP3363910B1 (fr) 2017-02-17 2017-02-17 Électrode de surveillance d'analyte continue avec enzyme réticulé
CN201880003937.0A CN109804076A (zh) 2017-02-17 2018-02-16 使用交联酶的连续分析物监测电极
JP2019543388A JP6801119B2 (ja) 2017-02-17 2018-02-16 架橋された酵素を用いた持続的な分析物モニタリング電極
RU2019127385A RU2731646C1 (ru) 2017-02-17 2018-02-16 Электрод с поперечно-сшитым ферментом для непрерывного контроля аналита
PCT/EP2018/053917 WO2018149981A1 (fr) 2017-02-17 2018-02-16 Électrode de surveillance continue d'analyte avec enzymes réticulées
CA3035410A CA3035410C (fr) 2017-02-17 2018-02-16 Electrode de surveillance continue d'analyte avec enzymes reticulees
US16/484,900 US11535881B2 (en) 2017-02-17 2018-02-16 Continuous analyte monitoring electrode with crosslinked enzyme

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP17156652.4A EP3363910B1 (fr) 2017-02-17 2017-02-17 Électrode de surveillance d'analyte continue avec enzyme réticulé

Publications (3)

Publication Number Publication Date
EP3363910A1 true EP3363910A1 (fr) 2018-08-22
EP3363910B1 EP3363910B1 (fr) 2023-12-06
EP3363910C0 EP3363910C0 (fr) 2023-12-06

Family

ID=58098490

Family Applications (1)

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EP17156652.4A Active EP3363910B1 (fr) 2017-02-17 2017-02-17 Électrode de surveillance d'analyte continue avec enzyme réticulé

Country Status (7)

Country Link
US (1) US11535881B2 (fr)
EP (1) EP3363910B1 (fr)
JP (1) JP6801119B2 (fr)
CN (1) CN109804076A (fr)
CA (1) CA3035410C (fr)
RU (1) RU2731646C1 (fr)
WO (1) WO2018149981A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020202825A (ja) * 2019-06-12 2020-12-24 王子ホールディングス株式会社 酵素固定化体及びそれを備えた測定装置ならびにアスパラギン及びl−アスパラギン酸の測定方法
CN114554955A (zh) * 2019-08-30 2022-05-27 美国雅培糖尿病护理公司 用于检测醇的分析物传感器和感测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6284126B1 (en) 1997-03-25 2001-09-04 Cygnus, Inc. Electrode with improved signal to noise ratio
WO2009153777A1 (fr) 2008-06-16 2009-12-23 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Électrode, procédé et système de détermination de la présence d'un analyte dans un fluide liquide
EP2348964B1 (fr) 2008-09-11 2013-05-15 Roche Diagnostics GmbH Système d'électrode pour mesurer une concentration de substance à analyser dans des conditions in vivo
EP2697388B1 (fr) 2011-03-28 2015-05-20 F.Hoffmann-La Roche Ag Couche de diffusion améliorée pour un capteur enzymatique in vivo

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS646856A (en) * 1987-06-30 1989-01-11 Toshiba Corp Enzyme immobilized electrode and its production
IL116921A (en) 1996-01-26 2000-11-21 Yissum Res Dev Co Electrochemical system for determination of an analyte in a liquid medium
JP3158181B2 (ja) * 1999-03-15 2001-04-23 北陸先端科学技術大学院大学長 バイオセンサー及び生体材料の固定化方法
JP3443768B2 (ja) 1999-06-21 2003-09-08 タニカ電器販売株式会社 酒燗器
JP2006225744A (ja) * 2005-02-21 2006-08-31 Hitachi Maxell Ltd 機能性粒子及びその製造方法
DE602006015638D1 (de) * 2005-06-07 2010-09-02 Canon Kk Struktur, poröser körper, sensor, strukturverfahren und nachweismethode für proben
JP4696723B2 (ja) * 2005-06-28 2011-06-08 ソニー株式会社 バイオセンサー
JP2008007719A (ja) 2006-06-30 2008-01-17 Toyobo Co Ltd ポリエステル樹脂水分散体及びこれを用いた接着剤、コーティング剤、塗料
WO2008007719A1 (fr) * 2006-07-12 2008-01-17 Arkray, Inc. Électrode à enzyme
EP2017350A1 (fr) * 2007-07-19 2009-01-21 F. Hoffmann-La Roche AG Capteur électrochimique doté d'une enzyme liée de manière covalente
WO2009039136A2 (fr) 2007-09-17 2009-03-26 Siometrix Corporation Dispositifs de détection produisant un signal à auto-activation et procédés
CA2755728A1 (fr) * 2009-03-16 2010-09-23 Arkray, Inc. Procede de mesure continue d'une concentration de substrat
RU117011U1 (ru) * 2011-12-22 2012-06-10 Федеральное государственное бюджетное учреждение науки Институт биохимии и физиологии микроорганизмов им. Г.К. Скрябина Российской академии наук (ИБФМ РАН) Устройство для определения содержания глюкозы на основе глюкозооксидазы penicillium adametzii и кислородного электрода типа кларка

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6284126B1 (en) 1997-03-25 2001-09-04 Cygnus, Inc. Electrode with improved signal to noise ratio
WO2009153777A1 (fr) 2008-06-16 2009-12-23 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd Électrode, procédé et système de détermination de la présence d'un analyte dans un fluide liquide
EP2348964B1 (fr) 2008-09-11 2013-05-15 Roche Diagnostics GmbH Système d'électrode pour mesurer une concentration de substance à analyser dans des conditions in vivo
EP2697388B1 (fr) 2011-03-28 2015-05-20 F.Hoffmann-La Roche Ag Couche de diffusion améliorée pour un capteur enzymatique in vivo

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BHARATHI S ET AL: "A glucose biosensor based on electrodeposited biocomposites of gold nanoparticles and glucose oxidase enzyme", ANALYST, ROYAL SOCIETY OF CHEMISTRY, vol. 126, no. 11, 1 November 2001 (2001-11-01), pages 1919 - 1922, XP002416667, ISSN: 0003-2654, DOI: 10.1039/B105318N *
CAO ET AL., BIOSENS. BIOELECTRON, vol. 26, 2010, pages 87 - 91
HUAAN ZHONG ET AL: "In situ chemo-synthesized multi-wall carbon nanotube-conductive polyaniline nanocomposites: Characterization and application for a glucose amperometric biosensor", TALANTA, ELSEVIER, AMSTERDAM, NL, vol. 85, no. 1, 17 March 2011 (2011-03-17), pages 104 - 111, XP028228545, ISSN: 0039-9140, [retrieved on 20110330], DOI: 10.1016/J.TALANTA.2011.03.040 *
OMER YEHEZKELI ET AL: "Integrated Oligoaniline-Cross-Linked Composites of Au Nanoparticles/Glucose Oxidase Electrodes: A Generic Paradigm for Electrically Contacted Enzyme Systems", CHEMISTRY - A EUROPEAN JOURNAL, vol. 15, no. 11, 2 March 2009 (2009-03-02), pages 2674 - 2679, XP055379018, ISSN: 0947-6539, DOI: 10.1002/chem.200801609 *

Also Published As

Publication number Publication date
CA3035410C (fr) 2023-02-28
CN109804076A (zh) 2019-05-24
EP3363910B1 (fr) 2023-12-06
JP6801119B2 (ja) 2020-12-16
EP3363910C0 (fr) 2023-12-06
US11535881B2 (en) 2022-12-27
WO2018149981A1 (fr) 2018-08-23
JP2020507773A (ja) 2020-03-12
RU2731646C1 (ru) 2020-09-07
US20190382819A1 (en) 2019-12-19
CA3035410A1 (fr) 2018-08-23

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