EP3283639B1 - Procédé pour la détermination de la diversité et de la viabilité des seuils utilisés pour évaluer des micro-organismes dans des échantillons de traitement - Google Patents
Procédé pour la détermination de la diversité et de la viabilité des seuils utilisés pour évaluer des micro-organismes dans des échantillons de traitement Download PDFInfo
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- EP3283639B1 EP3283639B1 EP16780726.2A EP16780726A EP3283639B1 EP 3283639 B1 EP3283639 B1 EP 3283639B1 EP 16780726 A EP16780726 A EP 16780726A EP 3283639 B1 EP3283639 B1 EP 3283639B1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/1826—Organic contamination in water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
Definitions
- Sequence by Synthesis means a technique used to determine the series of base pairs in DNA, also known as DNA sequencing. This sequencing method is based on reversible dye-terminators that enable the identification of single bases as they are introduced into DNA strands. Representative examples can be found at the websites: http://nxseq.bitesizebio.com/articles/sequencing-by-sythesis-explaining-the-illumina-sequencing-technology/ and http://www.illumina.com/technology/sequencing.technology.ilmn (as accessed on December 23, 2013).
- the method involves detecting a defect and then utilizing the PCR analysis to properly associate the source of the defect. In at least one embodiment the method determines if the defect is totally biologically based, totally non-biologically chemical based, or resulting from a combination of non-biologically chemical, mechanical, and biologically based sources.
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Toxicology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (11)
- Procédé d'anticipation d'un problème causé par un micro-organisme dans un système de traitement de l'eau, le procédé comprenant :la mesure de la population globale de micro-organismes dans au moins une partie du système ;la mesure de la quantité d'au moins un sous-groupe de la population de micro-organismes par rapport à la population globale de micro-organismes ;la détermination de savoir si la quantité d'au moins un sous-groupe de micro-organismes dépasse un seuil, le seuil étant une quantité prédéterminée :d'une quantité absolue du sous-groupe de micro-organismes, et/ou d'une quantité relative du sous-groupe de micro-organismes et leurs combinaisons ;dans lequel le procédé comprend en outre l'identification d'une source d'entrée qui est la cause de l'au moins un sous-groupe de la population de micro-organismes, etcomprenant en outre l'identification de la source d'eau dont provient le sous-groupe de micro-organismes et l'adoption d'une procédure de lutte biologique afin de remédier à la présence d'au moins un sous-groupe de micro-organismes uniquement après et au plus tard 1 semaine après l'introduction d'eau provenant de la source d'eau dont provient le sous-groupe de micro-organismes ;dans lequel le système de traitement d'eau comprend de l'eau d'alimentation provenant d'une source choisie dans le groupe constitué par: l'eau municipale, l'eau du robinet, l'eau de bassin, l'eau de mer, l'eau de lac, l'eau filtrée, l'eau dessalée, l'eau recyclée, les eaux usées, l'eau distillée, l'eau de chaudière condensée, l'eau de refroidissement, et l'une quelconque de leurs combinaisons.
- Procédé selon la revendication 1, dans lequel le sous-groupe de micro-organismes comprend des bactéries filamenteuses.
- Procédé selon les revendications 1 ou 2, dans lequel le sous-groupe de microorganismes comprend Spirogyra, Cladophora, Pithophora, O. Siphonocladales pithophora, Ulvibacter litoralis, Vetellibacter vladivostokensis, Weeksella virosa, Fucobacter, Gelidbacter, Ornithobacterium rhinotracheale, Riemerella cohambina, Flavobacterium psychrofilum, Herpetosiphon, Haliscomenobacter, Sphaerotilus, Ctyophaga succinicans, Vladibacter, Pfeifferella, Bacillus aquatilllis, Flexibacter marinus, Flavobacterium odoratum, Microscilla, Flexithrix, Capnocytophaga, Taxeobacter, Sporocytophaga, Saprospira, Chryseobacterium, Hymenobacter, et l'une quelconque de leurs combinaisons.
- Procédé selon l'une quelconque des revendications 1 à 3, dans lequel le seuil est le sous-groupe de micro-organismes en une quantité d'au moins 5 à 90 % de la population totale de micro-organismes.
- Procédé selon l'une quelconque des revendications 1 à 4, dans lequel au moins une mesure est prise au moyen d'un processus choisi dans la liste constituée de :séquençage par terminaison de chaîne, électrophorèse sur gel en gradient dénaturant (DGGE), réaction en chaîne par polymérase numérique, analyse basée sur l'ADN,séquençage de semi-conducteurs Ion torrent, MALDI, MALDI-TOF, spectrométrie de masse, analyse par PCR, pyroséquençage, analyse qPCR, séquençage par ligature etdétection d'oligonucléotides, séquençage par synthèse, mesure d'anticorps associés à un ou plusieurs micro-organismes, mesure d'hormones associées à un ou plusieurs micro-organismes, mesure de sécrétions associées à un ou plusieurs micro-organismes, mesure de protéines associées à un ou plusieurs micro-organismes, mesure de molécules organiques associées à un ou plusieurs micro-organismes, mesure de molécules associées à un ou plusieurs micro-organismes, phages fluorescents, trieurs de cellules, et l'une quelconque de leurs combinaisons
- Procédé selon l'une quelconque des revendications 1 à 5, dans lequel le système de traitement d'eau comprend une ou plusieurs étapes d'un élément choisi dans le groupe constitué par : la fabrication du papier, l'eau de refroidissement, l'eau de chaudière, la fabrication de produits alimentaires, la fabrication de boissons, le traitement de minerai, le traitement de l'alumine, la fabrication de biodiesel, la distillation, le raffinage pétrochimique, la synthèse pétrochimique, la synthèse de polymères, la fabrication de plastiques, le traitement des eaux usées, la blanchisserie, le lavage de vaisselle, le traitement de l'eau, la séparation solide-liquide, et l'une quelconque de leurs combinaisons.
- Procédé selon l'une quelconque des revendications 1 à 6, comprenant en outre l'enregistrement de l'organisme identifié en un format qui peut être stocké et/ou transmis.
- Procédé selon l'une quelconque des revendications 1 à 7, comprenant en outre la mise en œuvre d'un programme biocide associé à la remédiation de l'organisme identifié.
- Procédé selon l'une quelconque des revendications 1 à 8, dans lequel au moins une mesure est prise à partir d'un produit échantillon du système de traitement.
- Procédé selon la revendication 9, dans lequel l'échantillon est tellement desséché qu'il n'existe que peu ou pas d'organismes vivants sur celui-ci.
- Procédé selon l'une quelconque des revendications 1 à 10, dans lequel le sous-groupe de micro-organismes comprend des formateurs de biofilm.
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| PCT/US2016/027484 WO2016168430A1 (fr) | 2015-04-15 | 2016-04-14 | Procédé pour la détermination de la diversité et de la viabilité des seuils utilisés pour évaluer des micro-organismes dans des échantillons de traitement |
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| EP3283639A1 EP3283639A1 (fr) | 2018-02-21 |
| EP3283639A4 EP3283639A4 (fr) | 2018-11-21 |
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| CA (1) | CA2982587A1 (fr) |
| MA (1) | MA42192A (fr) |
| WO (1) | WO2016168430A1 (fr) |
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| WO2021250317A1 (fr) | 2020-06-09 | 2021-12-16 | Kemira Oyj | Procédés pour déterminer des bactéries et fabriquer une bande fibreuse et outils et utilisations afférents |
| WO2023137646A1 (fr) | 2022-01-20 | 2023-07-27 | Ecolab Usa Inc. | Procédé de nettoyage enzymatique permettant d'éliminer des micro-organismes ou un biofilm d'un équipement industriel |
| CN117630318B (zh) * | 2024-01-23 | 2024-04-09 | 四川省科源工程技术测试中心有限责任公司 | 电泳法测定水环境有机物含量的设备及方法 |
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Also Published As
| Publication number | Publication date |
|---|---|
| BR112017021943A2 (pt) | 2018-07-10 |
| JP2018512163A (ja) | 2018-05-17 |
| EP3283639A4 (fr) | 2018-11-21 |
| JP6892390B2 (ja) | 2021-06-23 |
| US11608516B2 (en) | 2023-03-21 |
| US20160304931A1 (en) | 2016-10-20 |
| CA2982587A1 (fr) | 2016-10-20 |
| EP3283639A1 (fr) | 2018-02-21 |
| WO2016168430A1 (fr) | 2016-10-20 |
| WO2016168430A8 (fr) | 2017-05-18 |
| CN107532193A (zh) | 2018-01-02 |
| MA42192A (fr) | 2018-04-18 |
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