Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/16—Amides, e.g. hydroxamic acids
  • A61K31/17—Amides, e.g. hydroxamic acids having the group >N—C(O)—N< or >N—C(S)—N<, e.g. urea, thiourea, carmustine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
  • A61K31/19—Carboxylic acids, e.g. valproic acid
  • A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
  • A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
  • A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
  • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
  • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/445—Non condensed piperidines, e.g. piperocaine
  • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
  • A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/47—Quinolines; Isoquinolines
  • A61K31/475—Quinolines; Isoquinolines having an indole ring, e.g. yohimbine, reserpine, strychnine, vinblastine
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
  • A61K31/57—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone
  • A61K31/573—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids substituted in position 17 beta by a chain of two carbon atoms, e.g. pregnane or progesterone substituted in position 21, e.g. cortisone, dexamethasone, prednisone or aldosterone
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/66—Phosphorus compounds
  • A61K31/675—Phosphorus compounds having nitrogen as a ring hetero atom, e.g. pyridoxal phosphate
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/69—Boron compounds
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
  • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
  • A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
  • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
  • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
  • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
  • A61K31/7064—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines
  • A61K31/7068—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom containing condensed or non-condensed pyrimidines having oxo groups directly attached to the pyrimidine ring, e.g. cytidine, cytidylic acid
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
  • A61K31/716—Glucans
  • A61K31/724—Cyclodextrins
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
  • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
  • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
  • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
  • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
  • A61K47/545—Heterocyclic compounds
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents

Definitions

• Anthocyanidin complex for the treatment of multiple myeloma is anthocyanidin complex for the treatment of multiple myeloma
• the invention relates to a complex of an anthocyanidin and a sulfoalkyl ether-.beta.-cyclodextrin and compositions comprising anthocyanidin or its salts as a medicament for the treatment of cancer.
• Anthocyanidins are zymochrome dyes antioxidati ⁇ ven properties that are found in most higher terrestrial plants.
• Anthocyanidins are sugar-free (aglycones) and are closely related to the sugary anthocyanins (glycosides), both of which fall under the generic term anthocyanins
• Plasma cells are cells of the immune system that produce antibodies for the fight against diseases and infections. These cells are transported through the bloodstream among other things in the bone marrow, accumulate there and cause permanent damage in healthy tissue, which manifests itself symptomatically by broken bones, increased calcium levels (hypercalcaemia) or even kidney failure.
• the cause of bone disease is the rapid proliferation of myeloma cells and release of Osteoklastenmodators IL-6, which activates the responsible for bone ⁇ substanzresorption osteoclasts, which leads result in damage to the bone and bone fractures.
• the object of the present invention is to provide an effective drug for the treatment of multiple myeloma.
• the complex of the invention or the inventive composition to ⁇ be used to treat an object or individual, which suffers from multiple myeloma.
• object includes live animals and humans.
• composition comprising at least one Anthocya ⁇ nidin includes anthocyanidin as such without further components.
• the purpose of this treatment is to at least partially kill or neutralize the myeloma cell. len.
• Negtralization and “killing” in the sense of the present invention means the at least partial destruction or dissolution or inactivation or prevention of the multiplication of myeloma cells.
• myeloma caused impairment of organs or tissues include hypercalcemia, stunning ⁇ tr campuste kidney function, anemia and bone injuries a.
• the asymptomatic myeloma closes the smoldering multiple myeloma (smoldering multiple myeloma, SMM) and Sta ⁇ dium I multiple myeloma.
• SMM is characterized by monoclonal protein and a slight increase in plasma cells in bone marrow.
• Indolent multiple myeloma (IMM) is characterized by low levels of monoclonal protein and an increased number of plasma cells in the bone marrow. Patients with multiple myeloma are also affected by their disease status. characterized.
• Responsive disease refers to myeloma, which responds to therapy in a manner that decreases M-protein levels by at least 50%;
• Stable disease refers to myeloma, which does not respond to treatment (ie does not reach 50% reduction in M protein levels), but does not progress, ie does not exacerbate;
• Progressive disease refers to active myeloma that looks worse, ie an increase of M protein levels and stronger influencer ⁇ tr founded
• Relapse disease refers to myeloma that initially responds to a Thera ⁇ pie but then again falls back into the progression state.
• Refractory disease refers to both myeloma, which is not responsive to first-line treatment, and relapsing myeloma, which is no longer responsive to subsequent treatments. In the latter case, one can also speak of a relapse disease.
• the complex or composition of the invention can be administered alone or in combination with at least one other therapeutic agent for reducing one or more symptoms of multiple myeloma.
• He invention ⁇ complex according to the present invention or together ⁇ reduction can simultaneously with the other therapeutic agent, which may be part of the same composition, or be provided in a different composition, may be administered.
• the erfindungsge ⁇ Permitted complex or composition of the invention before or after administration of another therapeutic agent can be administered.
• the complex of the invention or the composition according to the invention can be administered via the same or a different administration route as the walls ⁇ re therapeutic agents.
• the therapeutically ⁇ tables agents may be there ⁇ of chemotherapeutic agents, therapeutic ⁇ under supporting means, or a combination.
• “Chemotherapeutic agent” is an agent that is toxic to cancer cells.
• chemotherapeutic thera ⁇ apeutic agents which may find use in the present invention include bortezomib (Velca- DE®, Millennium), Melphatan, prednisone, vincristine, Car- mustin, cyclophosphamide, dexamethasone, thalidomide, Doxoru- bicine, cisplatin, etoposide, and cytarabine.
• the complex of the invention or the inventive together ⁇ men suffi in combination with bortezomib is (Velcade®) is used.
• antibiotics include sulfur-containing drugs, Penicillins (eg benzylpenicillin, P-hydroxybenzylpenicillin, 2-pentenylpenicillin, N-heptylpenicillin, phenoxymethylpenicillin, phenethicillin, methicillin, oxacillin, cloxacillin, dicloxacillin, flucoxacillino, nafcillin, ampicillin, amoxicillin, cyclacillin, carbenicillin, ticarcillin, piperacillin, azlocillin, mecz - locillin, mecillinam, amdinocillin), cephalosporin and their derivatives (eg cephalothin, cephapirin, cephacetrile,
• Alendronate (Fosamax), risedronate (Actonel), zoledronate (Zo ⁇ meta), ibandronate and (Boniva).
• diuretics include thiazide derivatives such as amiloride, chlorothiazide, hydrochlorothiazide, methylchlorothiazide and chlorothalidone.
• growth factors include granulocyte colony-stimulating factors (G-CSF), granulocytes.
• Macrophage colony stimulating factor GM-CSF
• macrophage colony stimulating factor M-CSF
• multicolor stimulating factors erythropoietin, thrombopoietin, oncostatin M and interleukins
• analgesics include opiates (e.g., morphine), COX-2 inhibitors (e.g., rofecoxib, valdecoxib, and celecoxib), salicylates (e.g., ASPIRIN, choline magnesium trisalicylate, salsalate, dirunisal, and sodium salicylate), propionic acid derivatives (e.g., fenoprofen calcium, ibuprofen,
• COX-2 inhibitors e.g., rofecoxib, valdecoxib, and celecoxib
• salicylates e.g., ASPIRIN, choline magnesium trisalicylate, salsalate, dirunisal, and sodium salicy
• Ketoprofen, naproxen and naproxen sodium indoleakenoic acid derivatives (e.g., indomethacin, sulfindac, etodalac and tolmentin), fenamates (e.g., mefenamic acid and meclofenamate), benzothiazine derivatives or oxicams (e.g., Mobic or Piroxicam) or pyrromolactic acid (e.g., ketorolac).
• indoleakenoic acid derivatives e.g., indomethacin, sulfindac, etodalac and tolmentin
• fenamates e.g., mefenamic acid and meclofenamate
• benzothiazine derivatives or oxicams e.g., Mobic or Piroxicam
• pyrromolactic acid e.g., ketorolac
• treatment in the context of the present invention is that all or part of reaching the nachfol ⁇ quietly mentioned results: the whole or partial reduction of the disease, improvement of at least one of the clinical symptoms or associated with the disease indicators, delay, suppression or protection against
• the object to be treated is a human or animal, preferably a mammal
• the veterinary treatment includes, in addition to the treatment of beneficial or wild animals (eg Sheep, cats, horses, cows, Pigs), laboratory animals (eg rats, mice, guinea pig ⁇ ne, monkeys).
• the object which is treated with the complex of the invention or the composition according to the invention and optionally further therapeuti ⁇ rule means is subjected to radiation under ⁇ and / or prepared for stem cell therapy.
• the complex according to the invention or the composition according to the invention can preferably be used in combination with optional further therapeutic agents in the context of an induction therapy in order to reduce the tumor burden prior to stem cell transplantation, but also in the context of stem cell transplantation and / or after stem cell transplantation.
• the complex of the invention or the invention to ⁇ composition are preferably provided as a pharmaceutical composition and administered to ⁇ .
• pharmaceutical composition encompasses one or more active ingredients and one or more inert ingredients which act as carriers for the active ingredient (s)
• the pharmaceutical compositions allow the complex or composition of the invention to be administered orally, parenterally, including
• a parenteral delivery form may be a solution, suspension or dispersion
• an ophthalmic, pulmonary or nasal delivery form may be, for example, an aerosol, solution, suspension or dispersion for the formulation and administration are known from the prior art, see, for example, "Remington's Pharmaceuti- cal Sciences "(Mack Publishing Co, Easton Pa.).
• compositions of the invention and complexes may be a subject (physiological salt solution, for example) can be administered intravenously using a pharmaceutically ⁇ table acceptable carrier.
• a formulation provides in aqueous Solution, preferably in physiologically acceptable buffers (eg, Hanks's solution, Ringer's solution, or physiologically buffered saline).
• physiologically acceptable buffers eg, Hanks's solution, Ringer's solution, or physiologically buffered saline.
• physiologically acceptable buffers eg, Hanks's solution, Ringer's solution, or physiologically buffered saline
• parenteral administration including intravenous, subcutaneous, intramuscular, and intraperitoneal administration, an aqueous or oily solution or solid formulation is also included consideration.
• the proportion of the active ingredient in the pharmaceutical composition may vary and is typically between 2 and 60 wt .-% of the dosage unit. the proportion of active compound is selected accordingly, so that an effective dose is he ⁇ ranges
• Salt or “pharmaceutically acceptable salt” means any pharmaceutically acceptable salt of a compound of the present invention which can release the pharmaceutically active agent or its active metabolite after administration. Salts of the compositions and complexes of the present invention may be derived from inorganic or organic acids and bases.
• the anthocyanidin can be used in “pure” or “purified”, which means that unwanted components have been removed.
• the substituents m of this formula are selected from the group consisting of hydrogen, hydroxy group and methoxy group.
• Cyclodextrins that may be complexed with the anthocyanidin according to the invention are cyclic oligosaccharides from -1, 4-glycosidically linked glucose molecules, ß-cyclodextrin has seven glucose units. At a
• Sulfoalkyether phenomenon are cyclodextrins in the art for increasing the hydrophilicity or
• Sulfoalkylether phenomenon contribute in particular to increase the stability of the complex of anthocyanidins and correspondingly substituted ß-cyclodextrin, so that so that the storage stability and formulability of particularly sensitive to oxidation
• Anthocyanidins is significantly improved.
• the Invent ⁇ modern complex can be formulated as a storage-stable aqueous solution or solid, will be shown in more detail below.
• Complexation with a sulfobutyl ether- ⁇ -cyclodextrin (SEB- ⁇ -CD) is particularly preferred according to the invention.
• SEB- ⁇ -CD sulfobutyl ether- ⁇ -cyclodextrin
• the degree of substitution of the cyclodextrin with sulfoalkyl ether groups is preferably 3 to 8, more preferably 4 to 8, more preferably 5 to 8, further preferably 6 to 7.
• Suitable sulfobutyl ether- ⁇ -cyclodextrins with a mean degree of substitution of 6 to 7 are, for example, in said WO 2009/134347 A2 and commercially available under the trade name Captisol®.
• anthocyanidins used according to the invention in pure, salted or complexed form are preferably selected from the group consisting of aurantinidine, cyanidin,
• the invention further provides an aqueous solution of the inventive composition or of the complex according to the invention for use as a medicament, in particular for the treatment of multiple myeloma.
• the preparation of the complex of the invention as well as a corresponding aqueous solution comprises the following Schrit ⁇ te:
• an aqueous solution is preferred
• Herge ⁇ represents containing 5 to 10 wt .-% of the cyclodextrin used. It is particularly preferred in the context of OF INVENTION ⁇ dung if the pH of the aqueous solution during or after, but preferably before the addition of anthocyanidins, preferably delphinidin, to a pH value of 7 or Weni ⁇ ger, preferably 6 or less , more preferably 5 or less, more preferably 4 to 5, is set. It has been shown that at this pH value ne higher concentration of the complex can be adjusted in aqueous solution.
• the concentration of the anthocyanidins calculated as chloride preferably at least 0.5 mg / ml, more preference ⁇ example at least 1.0 mg / ml, more preferably at least 1.5 mg / ml, more preferably 2.0 mg / ml.
• the particularly be ⁇ ferred concentration range of at least 2.0 mg / ml can be adjusted in particular in an aqueous solution having a pH between 4 and 5 a preferred embodiment.
• the mixing of the constituents of the aqueous solution can be effected by stirring, preferred mixing times are 2 to 20 h. Before ⁇ given to work in the dark, to avoid a Lichtinduz (7).
• Another object of the invention is a solid for use as a medicament, in particular for the treatment of multiple myeloma, which is obtainable according to the invention by removing the solvent from an aqueous solution according to the invention described above.
• the removal can preferably be carried out by freeze-drying (lyophilization).
• Both the aqueous drug solution of the invention and the drug solids have a high gerstabiltician La ⁇ .
• the column used was a Waters X Bridge TM C18.35 ⁇ , 150 mm x 4.6 mm.
• the mobile phases were as follows:
• UV-Vis detector 530 pm for the assay, 275 pm for the detection of contaminants
• Calibration solution A reference solution of delphinidin was prepared by weighing 10 mg delphinidin chloride into a 10 ml flask and dissolving in dilution solution 1. After dissolution, it was diluted approximately 10-fold with dilution solution 2 to make an approximate concentration of 0.1 mg / ml.
• the control calibration solution was prepared in the same manner. The calibration solutions were analyzed immediately by HPLC, since delphinidin chloride is unstable in solution.
• Neutral aqueous solutions containing 10% by weight of the respective cyclodextrin were prepared.
• a concentration of only 2% by weight was selected due to the lack of solubility.
• a complex of the invention is formulated as a solid.
• a complex of the invention is formulated as a solid.
• Example 3.1 Delphinidin / SBE- ⁇ -CD 5 g of SEB- ⁇ -CD were dissolved in 40 ml of distilled water to give a clear solution. The pH of the solution was adjusted to 4.8 by 1 M HCl. Subsequently, 0.11 g of delphinidin chloride was added and stirred for 2 h at 27 ° C in the dark. The homogeneous liquid was vacuum filtered through a 0.45 ⁇ m pore size cellulose nitrate membrane filter. The solution was frozen and then freeze-dried at -48 ° C and a pressure of about 10.3 Pa (77 mTorr). The lyophilizate was ground and sieved through a sieve of 0.3 mm mesh size.
• Example 3.2 Delphinidin / HPBCD
• Example 3.1 is according to the invention, examples 3.2 and 3.3 are comparison examples.
• Example 4 Stability Tests The solids according to Examples 3.1 to 3.3 were stored under the following conditions:
• Example 5 Stability Tests in Aqueous Solution To determine the level of delphinidin chloride in the delphinidin restroomn solutions a reverse phase HPLC method similar to the one already described above was used. The following reagents were used:
• the column used was a Waters X Bridge TM C18, 35 ⁇ , 150 mm x 4.6 mm.
• the mobile phases were as follows:
• Channel A water 770 ml, methanol 230 ml, formic acid 10 ml
• Channel B water 50 ml, methanol 950 ml, formic acid 10 ml
• Dilution solution 1 Mixture of 100 ml of methanol and 2.6 ml of 1 M HCl dilution solution 2: Mixture of 100 ml of 50% strength
• Calibration solution A reference solution of delphinidin was prepared by weighing 10 mg delphinidin chloride into a 10 ml flask and dissolving in dilution solution 1. After dissolution, it was diluted approximately 10-fold with dilution solution 2 to make an approximate concentration of 0.1 mg / ml.
• the control calibration solution was prepared in the same manner. The calibration solutions were analyzed immediately by HPLC, since delphinidin chloride is unstable in solution. Preparation of the test solutions:
• the delphinidine content was determined.
• the following table indicates the determined content as a percentage of the above-mentioned starting concentration.
• delphinidin + SBEBCD delphinidin
• the results of the BLI measurement are shown in FIGS. 1 to 11 and indicate the number of cells surviving the treatment.

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