EP2859103A2 - Ingénierie cellulaire à l'aide d'arn - Google Patents

Ingénierie cellulaire à l'aide d'arn

Info

Publication number
EP2859103A2
EP2859103A2 EP13728354.5A EP13728354A EP2859103A2 EP 2859103 A2 EP2859103 A2 EP 2859103A2 EP 13728354 A EP13728354 A EP 13728354A EP 2859103 A2 EP2859103 A2 EP 2859103A2
Authority
EP
European Patent Office
Prior art keywords
seq
mir
cell
rna
expression vector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP13728354.5A
Other languages
German (de)
English (en)
Other versions
EP2859103B1 (fr
Inventor
Lore Florin
Hitto Kaufmann
Angelika Hausser
Monilola Olayioye
Michaela STROTBEK
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim International GmbH
Original Assignee
Boehringer Ingelheim International GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim International GmbH filed Critical Boehringer Ingelheim International GmbH
Priority to EP19165808.7A priority Critical patent/EP3536789A1/fr
Priority to EP13728354.5A priority patent/EP2859103B1/fr
Publication of EP2859103A2 publication Critical patent/EP2859103A2/fr
Application granted granted Critical
Publication of EP2859103B1 publication Critical patent/EP2859103B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2884—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD44
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)
    • C12Y111/01007—Peroxidase (1.11.1.7), i.e. horseradish-peroxidase

Abstract

L'invention concerne le domaine de la technologie de culture cellulaire. Elle concerne un ARN ayant une séquence spécifique, des vecteurs d'expression codant pour ledit ARN, des lignées de cellules hôtes productrices comprenant ledit ARN, et des procédés de production de produits biopharmaceutiques recombinants à l'aide de la cellule hôte génétiquement modifiée ayant des niveaux modifiés desdits ARN, tels que des petits ARN non codants, de préférence des micro-ARN (mi ARN). L'invention concerne également des cellules hôtes génétiquement modifiées ayant des niveaux modifiés d'au moins un desdits ARN. Ces lignées cellulaires ont des caractéristiques améliorées de sécrétion et/ou de croissance en comparaison à des lignées cellulaires témoins.
EP13728354.5A 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn Active EP2859103B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP19165808.7A EP3536789A1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn
EP13728354.5A EP2859103B1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12171110 2012-06-06
EP13728354.5A EP2859103B1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn
PCT/EP2013/061465 WO2013182553A2 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP19165808.7A Division EP3536789A1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn

Publications (2)

Publication Number Publication Date
EP2859103A2 true EP2859103A2 (fr) 2015-04-15
EP2859103B1 EP2859103B1 (fr) 2019-04-17

Family

ID=48613588

Family Applications (2)

Application Number Title Priority Date Filing Date
EP13728354.5A Active EP2859103B1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn
EP19165808.7A Pending EP3536789A1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP19165808.7A Pending EP3536789A1 (fr) 2012-06-06 2013-06-04 Ingénierie cellulaire à l'aide d'arn

Country Status (3)

Country Link
US (3) US20150140591A1 (fr)
EP (2) EP2859103B1 (fr)
WO (1) WO2013182553A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112272702A (zh) * 2017-12-27 2021-01-26 财团法人生物技术开发中心 具有增强的蛋白质表达效率的宿主细胞及其用途

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2859103B1 (fr) 2012-06-06 2019-04-17 Boehringer Ingelheim International GmbH Ingénierie cellulaire à l'aide d'arn
EP2937417B1 (fr) * 2014-04-25 2019-02-20 Hochschule Biberach Productivité de cellules améliorant des miARN
MA40782A (fr) 2014-10-03 2017-08-08 Hocuslocus Llc Procédés et compositions utilisant un arn non codant en vue de la culture et de la sélection de cellules
US20170369556A1 (en) * 2014-12-23 2017-12-28 Ralph A. Tripp RECOMBINANT CELLS COMPRISING miRNA MIMICS
WO2017157593A1 (fr) 2016-03-18 2017-09-21 Boehringer Ingelheim International Gmbh Cellules de mammifère destinées à la production d'une protéine sécrétée

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ATE159548T1 (de) 1990-11-13 1997-11-15 Immunex Corp Bifunktionelle wählbare fusionsgene
WO1994028143A1 (fr) 1993-05-21 1994-12-08 Targeted Genetics Corporation Genes de fusion selectables et bifonctionnels se basant sur le gene de cytosine-deaminase (cd)
ES2293096T3 (es) 2002-11-29 2008-03-16 BOEHRINGER INGELHEIM PHARMA GMBH & CO. KG Nuevos genes de la fosfotransferasa de neomicina y procedimiento para la seleccion de celulas recombinantes de produccion elevada.
US7700284B2 (en) * 2004-11-10 2010-04-20 Attagene, Inc. Reporter transcription unit populations and kits comprising same
JP2009545309A (ja) * 2006-08-04 2009-12-24 ダブリン シティ ユニバーシティ 組換え型生物学的製剤を製造する方法
WO2008061537A2 (fr) * 2006-11-23 2008-05-29 Querdenker Aps Oligonucléotides pour moduler l'activité d'arn cible
US7943549B2 (en) * 2007-04-02 2011-05-17 Georgia State University Research Foundation, Inc. Biological-based catalyst to delay plant development processes
JPWO2009044899A1 (ja) * 2007-10-03 2011-02-17 協和発酵キリン株式会社 細胞の増殖を制御する核酸
WO2010036939A2 (fr) * 2008-09-26 2010-04-01 University Of Southern California Système d'expression synergiste de petits éléments d'arn fonctionnels multiples
EP2356140A2 (fr) * 2008-10-07 2011-08-17 Novimmune Sa Procédés de transfection médiée par il-17
JP2012519484A (ja) * 2009-03-06 2012-08-30 マウント・シナイ・スクール・オヴ・メディシン マイクロｒｎａ応答要素を含む弱毒化生インフルエンザウイルスワクチン
US8815586B2 (en) * 2009-04-24 2014-08-26 The Board Of Regents Of The University Of Texas System Modulation of gene expression using oligomers that target gene regions downstream of 3′ untranslated regions
WO2011032100A1 (fr) * 2009-09-11 2011-03-17 Government Of The U.S.A., As Represented By The Secretary, Department Of Health And Human Services Inhibiteurs du kshv vil6 et il6 humain
EP2611918A1 (fr) * 2010-05-28 2013-07-10 Vereniging Voor Christelijk Hoger Onderwijs, Weten Arnmi impliqués dans la fonction de barrière hémato-encéphalique
AU2011288262A1 (en) * 2010-08-13 2013-04-04 The University Court Of The University Of Glasgow Therapeutic uses of microvesicles and related microRNAs
EP3372684B1 (fr) * 2010-08-24 2020-10-07 Sirna Therapeutics, Inc. Agents d'arni à un seul brin contenant un espaceur interne d'acide non nucléique
EP2859103B1 (fr) 2012-06-06 2019-04-17 Boehringer Ingelheim International GmbH Ingénierie cellulaire à l'aide d'arn
WO2017157593A1 (fr) * 2016-03-18 2017-09-21 Boehringer Ingelheim International Gmbh Cellules de mammifère destinées à la production d'une protéine sécrétée

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112272702A (zh) * 2017-12-27 2021-01-26 财团法人生物技术开发中心 具有增强的蛋白质表达效率的宿主细胞及其用途

Also Published As

Publication number Publication date
WO2013182553A2 (fr) 2013-12-12
EP3536789A1 (fr) 2019-09-11
US20220064690A1 (en) 2022-03-03
US20150140591A1 (en) 2015-05-21
WO2013182553A3 (fr) 2014-01-30
US20180119191A1 (en) 2018-05-03
EP2859103B1 (fr) 2019-04-17
US11230725B2 (en) 2022-01-25

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