EP2821138B2 - Flusszelle mit integrierter Trockensubstanz - Google Patents

Flusszelle mit integrierter Trockensubstanz Download PDF

Info

Publication number
EP2821138B2
EP2821138B2 EP13175335.2A EP13175335A EP2821138B2 EP 2821138 B2 EP2821138 B2 EP 2821138B2 EP 13175335 A EP13175335 A EP 13175335A EP 2821138 B2 EP2821138 B2 EP 2821138B2
Authority
EP
European Patent Office
Prior art keywords
flow cell
cavity
support element
passage
cell according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP13175335.2A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP2821138B1 (de
EP2821138B8 (de
EP2821138A1 (de
Inventor
Lutz Weber
Tina Röser
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thinxxs Microtechnology GmbH
Original Assignee
Thinxxs Microtechnology GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=48745809&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2821138(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to ES13175335T priority Critical patent/ES2704424T5/es
Priority to EP13175335.2A priority patent/EP2821138B2/de
Priority to DK13175335.2T priority patent/DK2821138T4/da
Application filed by Thinxxs Microtechnology GmbH filed Critical Thinxxs Microtechnology GmbH
Priority to PCT/EP2014/064290 priority patent/WO2015001070A1/de
Priority to CN201480046983.0A priority patent/CN105517710B/zh
Priority to US14/902,787 priority patent/US10232367B2/en
Publication of EP2821138A1 publication Critical patent/EP2821138A1/de
Publication of EP2821138B1 publication Critical patent/EP2821138B1/de
Priority to US16/265,127 priority patent/US10946376B2/en
Publication of EP2821138B8 publication Critical patent/EP2821138B8/de
Application granted granted Critical
Publication of EP2821138B2 publication Critical patent/EP2821138B2/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/04—Exchange or ejection of cartridges, containers or reservoirs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/163—Biocompatibility

Definitions

  • the invention relates to a flow cell with a dry substance arranged in a cavity within the flow cell, the flow cell having the features of claim 1 .
  • Microfluidic flow cells as they are increasingly used as a "mini laboratory" for the analysis and/or synthesis of fluids, particularly in diagnostics, contain reaction substances in liquid and/or solid form that are to be introduced into the flow cells during the production of the flow cells.
  • a reagent liquid to be dried ie a carrier liquid with a dissolved or suspended reagent.
  • the entire component of the flow cell that is only partially wetted with the reagent liquid must be subjected to a drying process before further assembly, which is often combined with a heat treatment for the purpose of acceleration or is carried out as a freeze-drying process to protect the reagents and with regard to stability and resuspension properties.
  • the disadvantage is that the component, whose dimensions usually far exceed those of the area that is only to be dried, occupies a lot of space in a drying chamber. Furthermore, the drying treatment can affect this component of the flow cell itself, particularly sensitive components mounted thereon. Above all, the dry substance formed can then be subject to impairments due to air contact, in particular humidity, and welding heat or the influence of adhesives used during assembly in the course of final assembly of the flow cell, through which the corresponding channel areas of a microfluidic flow cell must be hermetically sealed in many cases.
  • a method for introducing a dry substance into a flow cell, as described above, is z. B. from the EP 2 198 964 B1 out.
  • One from the WO 2012/154306 A1 known flow cell has a cavity that is accessible after opening a hatch or flap and has a recess in a floor for receiving a cuboid support element. The hatch or flap must be closed when the flow cell is in operation.
  • a flow cell with the features of the preamble of claim 1 is in each case from WO 2011/051735 A2 and WO 2008/134462 A1 out.
  • the object of the invention is to create a new microfluidic flow cell of the type mentioned at the outset with an integrated dry substance that can be produced more easily than in the prior art without adversely affecting the dry substance or other components of the flow cell due to the production environment.
  • the dry substance can be formed by drying a reagent liquid separately from the rest of the flow cell on a carrier element, which is used solely to hold the dry substance and allows the dry substance to be introduced into the flow cell in a final assembly step. Impairment of flow cell components by the drying process and impairment of the dry reagent introduced by further assembly work on the flow cell are eliminated.
  • the carrier element can have significantly smaller dimensions than the flow cell, with the dimensions of the carrier element being based on the size of the area carrying the dry reagent. Coatings that promote adhesion of the dry substance to its carrier surface can advantageously remain limited to the carrier surface of the carrier element, so that impairments of welded or adhesive connections by such coatings can be ruled out.
  • the cavity can form a channel network for the transport, analysis and/or synthesis of a fluid.
  • carrier elements possibly with different dry substances, can be used in the flow cell.
  • the cavity is delimited by a recess in a plate-shaped substrate and a foil-like cover closing the recess.
  • the passage is formed in the substrate, which is thicker than the film-like cover.
  • the carrier element is preferably designed in such a way that it can be detachably and/or non-detachably connected to the flow cell while closing the cavity.
  • the shape of the passage is preferably adapted to the shape of the carrier element. Fluid-tightness can be achieved in particular by welding and/or gluing, possibly also just by mechanically pressing the carrier element into the passage.
  • the support element expediently fills the passage completely, at least in cross section, with the support element and the passage preferably having a circular shape in cross section, which is advantageous in terms of production technology.
  • the carrier element tapers towards the cavity, while the passage narrows.
  • a tight closure of the cavity in the form of a press fit can be achieved simply by mechanically pressing the carrier element into the passage.
  • the carrier element preferably has a section which protrudes outwards from the flow cell and which can serve as a grip part to simplify manual handling or automated assembly.
  • the protruding section can overlap the flow cell on the outside in the manner of a collar, with the collar being able to achieve an additional sealing of the cavity.
  • the carrier element can be screwed into the passage.
  • the support surface of the support member may be flush with or set back from an adjacent wall surface of the cavity. Alternatively, the support member protrudes into the cavity from the adjacent wall surface.
  • the carrier surface expediently has a structuring, coating and/or surface modification that promotes adhesion of the dry substance.
  • the carrier element and the carrier surface carrying the dry reagent are preferably made of plastic.
  • the carrier surface can be formed by a separate surface component made of glass, silicon, ceramic or metal that is connected to the rest of the carrier element and can be applied by means of welding or gluing. This is advantageous if surface properties other than those that can be realized by a plastic surface or coating are necessary for the application of the dry reagent.
  • Dry reagents include salts, buffers, e.g. for cell lysis, magnetic and non-magnetic beads, enzymes, antibodies, DNA fragments, proteins, PCR reagents or alternatively cells.
  • the flow cell shown as a detail comprises a plate-shaped substrate 1 with a recess 2 which is covered by a film 4 bonded and/or welded to the substrate, forming a cavity 3 .
  • the cavity 3 is part of an in 1 Otherwise not shown channel network of the flow cell, in particular it forms a channel area in which a dry reagent 5 containing antibodies, for example, adheres to a channel wall 6 .
  • the dry reagent 5 originates from a reagent liquid 7 introduced by dispensing before the recess 2 is covered by the film 4 into the recess 2 forming a channel or chamber region of the flow cell.
  • a heat treatment or /and subjected to freeze drying was subjected to a heat treatment or /and subjected to freeze drying.
  • Out 2 discloses a method for introducing a dry substance, in particular a dry reagent 5, into a flow cell, in which the dry reagent 5 is applied to a separate carrier part 8.
  • a cavity 3 in a flow cell which is, for example, an area of an in 3 Channel 9 shown can act, has a through-opening 10 into which the carrier element 8 with a conical section 11 having a carrier surface 13 for the dry reagent 5 can be inserted with fluid-tight closure of the cavity 3 .
  • the carrier surface 13 forms part of the wall surface of the cavity 3.
  • a fluid transported or processed in the cavity 3 can thus interact with the dry reagent; in particular, the dry reagent can be dissolved and resuspended by the fluid.
  • components of the fluid such as cells or analytes can interact with the dry reagent and/or bind to it, in that the fluid flows over the carrier surface, possibly several times changing the direction of transport.
  • the carrier element 8 fitted into the through-opening 10 can be glued or welded to the substrate.
  • a section 12 of the carrier element that protrudes beyond the through-opening 10 on the side of the substrate 1 facing away from the cavity 3 8 serves as the mounting of the carrier element 8 facilitating handle part.
  • the meandering channel 9 serves to redissolve the dry reagents 5 introduced by the carrier elements 8 by flushing them alternately.
  • the substrate 1 and the film 4 of the flow cell are preferably made of a plastic, in particular both of the same plastic, with PMMA, PC, PS, PEEK, PP, PE, COC and COP, for example, being suitable for this purpose.
  • the carrier element 8 is also preferably a plastic part, which in particular consists of the same plastic as the substrate. The production of the substrate and the carrier elements from plastic is expediently carried out by injection molding.
  • the carrier surface 13 of the carrier element 8 accommodating the dry reagent 5 can be flush with the adjacent wall surface 14 of the cavity 3 or set back to this wall surface.
  • the carrier element 8 can also protrude with the carrier surface 13 into the cavity 3 .
  • This can be advantageous in order to generate turbulence locally in a laminar flow that is normally present in microchannels by abruptly changing the channel cross section and/or in order to increase the flow rate of the fluid in the channel area into which the carrier element 8 is introduced by reducing the channel cross section achieve, for example, to accelerate and control a redissolution of the dry reagent.
  • assembly and/or component tolerances can be compensated for.
  • figure 5 shows further embodiments for carrier elements 8 according to Figure 5a cylindrical and according Figure 5b can be cylindrical with a collar 15 engaging behind the substrate 1 .
  • Figure 5c shows an embodiment of a cylindrical support element 8 having a collar 13 and having an external thread 16 which engages in an internal thread in the passage opening in question.
  • the carrier element 8 can advantageously be detached from the flow cell, provided that there is no adhesive bonding or welding to the substrate 1 in addition to the screw connection.
  • the solubility can be advantageous if the dry reagent is to be separated from the flow cell again after interaction with the fluid and subjected to further analysis.
  • a carrier element 8 which can be detached from the flow cell and has an extended handle part 17 is shown Figure 5e .
  • the carrier element 8 can be pressed into the relevant through-opening in the substrate 1 while sealing the cavity 3 fluid-tight.
  • the guidance of the carrier element 8 can be improved.
  • Figure 5d shows a carrier element 8 with a conical section and a collar 15 protruding from a through-opening, which is sealed against the substrate 1 by an annular seal 18 .
  • the rotationally symmetrical carrier elements can have a marking that makes it possible to insert the carrier elements into the passage in a desired rotational position.
  • Figure 6a has a carrier element with a depression 19 for receiving a dry reagent 5 .
  • a carrier surface 13 is formed with a multiplicity of receiving depressions in the form of grooves 20 arranged crosswise with typical cross-sectional dimensions of 0.01 ⁇ 0.01 mm 2 to 1 ⁇ 1 mm 2 for a dry reagent.
  • the surface of the carrier surface 13 can be enlarged in a simple manner, so that either a larger quantity of dry reagent 5 can be accommodated with the same dimensions of the carrier element 8 and/or the dry substance can dry more homogeneously than a large drop on a smooth carrier surface and/or or the microstructure of the support surface 13 formed by the receiving depressions 20 can generate turbulence when the fluid flows over it, which positively influence the release behavior.
  • the grooves could be in the form of concentric circles.
  • Figure 6c shows a receiving surface with a porous element 21 applied to the support surface by clamping, gluing or welding, in which a dry substance can settle.
  • the porous element 21 advantageously forms an enlarged surface for receiving the dry reagent 5.
  • Figure 6d has a carrier element with a treated carrier surface, wherein the treatment can be, for example, a wet-chemical treatment, a plasma treatment or a corona treatment.
  • the treatment can be carried out, for example, by means of plasma polymerisation or lead to a coating 22 by means of a PVD process, for example a glass or metal coating.
  • the carrier component shown is formed in two parts with a separate surface component 26 .
  • the surface component 26 forming the support surface does not consist, for example, of a plastic, as is preferred for the rest of the support component, but of glass, silicon, metal or ceramic. If the functionalization, ie the application of the dry reagent to the carrier surface, requires such materials, as is the case with protein (e.g. antibodies) or nucleic acid-based analysis technologies, the use of these materials, which are often significantly more expensive than plastic, is limited. advantageously only on a surface area, with dimensions of 0.5 ⁇ 0.5 mm to 5 ⁇ 5 mm and thicknesses between 0.1 and 1 mm being considered.
  • the surface component 26 can be attached to the rest of the support component by means of clamps or by gluing or welding.
  • a large number of carrier elements 8 can be processed simultaneously by the carrier elements 8 being arranged in step 7a on a carrier tablet 24 having rows of holes 23.
  • a layer 22 that improves the adhesion of a substance is produced simultaneously on all carrier surfaces 13 of the carrier elements 8 .
  • the coating can also cover other surface areas of the carrier element 8 that are not intended for application of the dry reagent 5 .
  • a drying treatment takes place, so that the dry substance 5 adhering thereto is deposited on the layers 22.
  • step 7e the finished carrier elements 8 provided with a dry substance 5 can be removed for processing.
  • the carrier element 8 has a carrier surface for a dry substance 5 which is formed by a membrane 27 .
  • This membrane can be connected in one piece to the rest of the carrier element 8 or formed by a separate component connected to the rest of the carrier element, which preferably consists of the same plastic as the rest of the carrier element.
  • the membrane 27 is transparent, which closes off a through opening 28 formed in the carrier element 8 at one end, there is the possibility of optical detection of the interaction of the fluid with the dry substance 5 in accordance with Figure 8b to control. Furthermore, according to Figure 8c the possibility of forming the membrane 27 convex or concave by pneumatic or mechanical pressurization.
  • the interaction between the dry substance and the fluid can be stimulated in particular by alternating bulging and inward arching of the membrane 27 and both improved resuspension of dry substances and improved accumulation of components of the fluid on dry substances, eg in the case of antibodies, can be achieved.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Secondary Cells (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP13175335.2A 2013-07-05 2013-07-05 Flusszelle mit integrierter Trockensubstanz Active EP2821138B2 (de)

Priority Applications (7)

Application Number Priority Date Filing Date Title
ES13175335T ES2704424T5 (es) 2013-07-05 2013-07-05 Célula de flujo con sustancia seca integrada
EP13175335.2A EP2821138B2 (de) 2013-07-05 2013-07-05 Flusszelle mit integrierter Trockensubstanz
DK13175335.2T DK2821138T4 (da) 2013-07-05 2013-07-05 Flydecelle med integreret tørstof
PCT/EP2014/064290 WO2015001070A1 (de) 2013-07-05 2014-07-04 Flusszelle mit integrierter trockensubstanz
CN201480046983.0A CN105517710B (zh) 2013-07-05 2014-07-04 具有整合的干燥物质的液流电池
US14/902,787 US10232367B2 (en) 2013-07-05 2014-07-04 Flow cell with an integrated dry substance
US16/265,127 US10946376B2 (en) 2013-07-05 2019-02-01 Carrier element for introducing a dry substance into a flow cell

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP13175335.2A EP2821138B2 (de) 2013-07-05 2013-07-05 Flusszelle mit integrierter Trockensubstanz

Publications (4)

Publication Number Publication Date
EP2821138A1 EP2821138A1 (de) 2015-01-07
EP2821138B1 EP2821138B1 (de) 2018-10-24
EP2821138B8 EP2821138B8 (de) 2019-03-06
EP2821138B2 true EP2821138B2 (de) 2022-02-09

Family

ID=48745809

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13175335.2A Active EP2821138B2 (de) 2013-07-05 2013-07-05 Flusszelle mit integrierter Trockensubstanz

Country Status (6)

Country Link
US (1) US10232367B2 (da)
EP (1) EP2821138B2 (da)
CN (1) CN105517710B (da)
DK (1) DK2821138T4 (da)
ES (1) ES2704424T5 (da)
WO (1) WO2015001070A1 (da)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2516669B (en) 2013-07-29 2015-09-09 Atlas Genetics Ltd A method for processing a liquid sample in a fluidic cartridge
GB2516666B (en) 2013-07-29 2015-09-09 Atlas Genetics Ltd Fluidic cartridge for nucleic acid amplification and detection
GB2516672B (en) 2013-07-29 2015-05-20 Atlas Genetics Ltd A system and method for expelling liquid from a fluidic cartridge
GB2516675A (en) 2013-07-29 2015-02-04 Atlas Genetics Ltd A valve which depressurises, and a valve system
GB2516667A (en) 2013-07-29 2015-02-04 Atlas Genetics Ltd An improved cartridge, cartridge reader and method for preventing reuse
CN105980058A (zh) 2014-01-07 2016-09-28 达克雷诊断器材有限公司 流体输送装置、系统和方法
CN107002016A (zh) 2014-09-29 2017-08-01 奇普凯尔公司 包括匣盒和流体芯片的用于细胞的光学检测的设备及其方法
EP3224594A4 (en) 2014-11-28 2018-08-08 Chipcare Corporation Multiplex bead array assay
EP3108962A1 (de) * 2015-06-22 2016-12-28 Thinxxs Microtechnology Ag Probenträger
EP3199240A1 (de) 2016-01-26 2017-08-02 ThinXXS Microtechnology AG Mikrofluidische flusszelle mit integrierter elektrode und verfahren zu ihrer herstellung
EP3263215B1 (de) * 2016-06-30 2021-04-28 ThinXXS Microtechnology AG Vorrichtung mit einer flusszelle mit reagenzspeicher
US20200009555A1 (en) 2016-10-07 2020-01-09 Boehringer Ingelheim Vetmedica Gmbh Cartridge for testing a sample and method for producing a cartridge of this kind
WO2018122852A1 (en) 2016-12-29 2018-07-05 Schnell Amit Cartridge for use in in-vitro diagnostics and method of use thereof
EP3342485B1 (de) 2017-01-02 2020-07-08 Thinxxs Microtechnology Ag Halterung für reagenzträgerelemente
EP3444034A1 (en) 2017-08-18 2019-02-20 XanTec bioanalytics GmbH Flow cell for the selective enrichment of target particles or cells
US10046322B1 (en) * 2018-03-22 2018-08-14 Talis Biomedical Corporation Reaction well for assay device
GB201819415D0 (en) 2018-11-29 2019-01-16 Quantumdx Group Ltd Microfluidic apparatus and method
US11008627B2 (en) 2019-08-15 2021-05-18 Talis Biomedical Corporation Diagnostic system
GB201917832D0 (en) 2019-12-05 2020-01-22 Oxford Nanopore Tech Ltd Microfluidic device for preparing and analysing a test liquid
EP4173708A1 (de) 2021-10-28 2023-05-03 thinXXS Microtechnology GmbH Mikrofluidelement, insbesondere flusszelle, mit integriertem trockenreagenz
WO2024038109A1 (de) * 2022-08-17 2024-02-22 Thinxxs Microtechnology Gmbh Mikrofluidische flusszelle, herstellungsverfahren, verwendung und analyseeinrichtung

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008134462A1 (en) † 2007-04-25 2008-11-06 3M Innovative Properties Company Supported reagents, methods, and devices
WO2011051735A2 (en) † 2009-11-02 2011-05-05 The Secretary Of State For Environment, Food & Rural Affairs .. Device and apparatus

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5725831A (en) * 1994-03-14 1998-03-10 Becton Dickinson And Company Nucleic acid amplification apparatus
US6488828B1 (en) * 2000-07-20 2002-12-03 Roche Diagnostics Corporation Recloseable biosensor
JP4013671B2 (ja) * 2002-07-05 2007-11-28 松下電器産業株式会社 ポリメラーゼ連鎖反応容器及びその製造方法
DE10336849A1 (de) 2003-08-11 2005-03-10 Thinxxs Gmbh Flusszelle
DE102004021780B4 (de) * 2004-04-30 2008-10-02 Siemens Ag Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien
WO2006042734A1 (de) * 2004-10-15 2006-04-27 Siemens Aktiengesellschaft Verfahren zur durchführung einer elektrochemischen messung an einer flüssigen messprobe in einer über leitungen zugänglichen messkammer und zugehörige anordnung
MX2008008280A (es) * 2005-12-21 2008-11-06 Meso Scale Technologies Llc Modulos de ensayo que tienen reactivos de ensayo y metodos para hacer y utilizar los mismos.
DE102008021364A1 (de) 2008-04-29 2009-06-25 Siemens Aktiengesellschaft Verfahren zum Einbringen von Trockenreagenzien in eine Analyseeinheit und Kit
KR101102532B1 (ko) 2008-07-10 2012-01-03 삼성전자주식회사 시약 카트리지, 시약 카트리지를 구비하는 미세유동장치, 그 제조방법, 및 이를 이용한 시료분석방법
EP2198964B8 (de) 2008-11-06 2013-04-24 F. Hoffmann-La Roche AG Verfahren zum Bereitstellen eines getrockneten Reagenz in einem mikrofluidischen System
WO2011094577A2 (en) 2010-01-29 2011-08-04 Micronics, Inc. Sample-to-answer microfluidic cartridge
GB2483077A (en) * 2010-08-25 2012-02-29 Concateno Uk Ltd Sample testing assay apparatus and method
CN107469878B (zh) * 2011-04-13 2021-01-15 阿科尼生物系统公司 基于微阵列的样检系统
US20140174926A1 (en) * 2011-05-02 2014-06-26 Advanced Liquid Logic, Inc. Molecular diagnostics platform
US9709580B2 (en) * 2011-05-12 2017-07-18 William Marsh Rice University Bio-nano-chips for on-site drug screening
EP2610009A1 (en) 2011-12-29 2013-07-03 Samsung Electronics Co., Ltd Solid reagent dissolving device and method of dissolving solid reagent by using the same
US20150241319A1 (en) * 2012-09-26 2015-08-27 Thomas N. Chiesl Swab interface for a microfluidic device

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008134462A1 (en) † 2007-04-25 2008-11-06 3M Innovative Properties Company Supported reagents, methods, and devices
WO2011051735A2 (en) † 2009-11-02 2011-05-05 The Secretary Of State For Environment, Food & Rural Affairs .. Device and apparatus

Also Published As

Publication number Publication date
ES2704424T3 (es) 2019-03-18
US10232367B2 (en) 2019-03-19
EP2821138B1 (de) 2018-10-24
CN105517710A (zh) 2016-04-20
CN105517710B (zh) 2017-04-05
DK2821138T4 (da) 2022-05-16
DK2821138T3 (da) 2019-02-11
EP2821138B8 (de) 2019-03-06
ES2704424T5 (es) 2022-05-20
US20160167047A1 (en) 2016-06-16
WO2015001070A1 (de) 2015-01-08
EP2821138A1 (de) 2015-01-07

Similar Documents

Publication Publication Date Title
EP2821138B2 (de) Flusszelle mit integrierter Trockensubstanz
EP2576065B1 (de) Flusszelle mit hohlraum und diaphragma
DE19948087B4 (de) Verfahren zur Herstellung eines Reaktionssubstrats
EP2437890A1 (de) Vorrichtung zum transportieren eines fluids in einem kanalstrang eines mikrofluidelements
WO2001005505A1 (de) Einrichtung zum handhaben von flüssigkeitsproben und herstellungsverfahren sowie system zum handhaben von flüssigkeitsproben
EP1846160A1 (de) Neuartige mikrofluidische probenträger
EP3143119B1 (de) Vorrichtung und verfahren zum aufbereiten einer biologischen probe und analysesystem zum analysieren einer biologischen probe
EP2248588B1 (de) Montier- und demontierbares Mikrofluidiksystem und Verfahren zur Flutung des Systems
DE112015002018T5 (de) Vorrichtung zum Lagern von biochemischen Reagenzien und biochemischer Analysator
DE19910392B4 (de) Mikrosäulenreaktor
DE102009001257A1 (de) Vorrichtung und Verfahren zur Handhabung von Flüssigkeiten
EP1489404A1 (de) Verfahren zum Herstellen einer 3-D-Mikroskop-Durchflusszelle
DE10152690A1 (de) Durchflussreaktionskammer für Sensorchips
US10946376B2 (en) Carrier element for introducing a dry substance into a flow cell
DE102009001261A1 (de) Vorrichtung und Verfahren zur Ausführung mehrerer paralleler PCR-Reaktionen im Durchflussverfahren
WO2014060998A1 (de) Integriertes mikrofluidisches bauteil zur anreicherung und extraktion biologischer zellbestandteile
EP3070160A1 (de) Vorrichtung und verfahren zum bearbeiten einer zielzellen enthaltenden probe biologischen materials
EP3094740B1 (de) Analyseeinheit zum durchführen einer verschachtelten polymerasekettenreaktion, analysevorrichtung, verfahren zum betreiben einer solchen analyseeinheit und verfahren zum herstellen einer solchen analyseeinheit
DE102014221309A1 (de) Mikrofluidisches System sowie Verfahren zum Analysieren einer Probenlösung und Verfahren zum Herstellen eines mikrofluidischen Systems zum Analysieren
DE102011080527A1 (de) Lateral durchströmtes Chromatographieelement
DE102014207888A1 (de) Vorrichtung und Verfahren zur Durchführung von biochemischen Prozessen
WO2021063893A1 (de) Glasverbundmaterial und verfahren zur herstellung eines glasverbundmaterials
WO2021013595A1 (de) Lab-on-chip-system mit mindestens einem funktionalisierten abschnitt
DE102006049560A1 (de) Trägerelement und Verfahren zur Herstellung desselben
DE102014221412A1 (de) Analyseeinheit zum Analysieren eines Fluids, Analysevorrichtung, Verfahren zum Betreiben einer Analyseeinheit und Verfahren zum Herstellen einer Analyseeinheit

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20130705

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

RBV Designated contracting states (corrected)

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

17Q First examination report despatched

Effective date: 20151026

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20171106

GRAJ Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

INTC Intention to grant announced (deleted)
GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

GRAJ Information related to disapproval of communication of intention to grant by the applicant or resumption of examination proceedings by the epo deleted

Free format text: ORIGINAL CODE: EPIDOSDIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: EXAMINATION IS IN PROGRESS

INTG Intention to grant announced

Effective date: 20180125

INTC Intention to grant announced (deleted)
RIN1 Information on inventor provided before grant (corrected)

Inventor name: LUTZ, WERER

Inventor name: ROESER, TINA

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: GRANT OF PATENT IS INTENDED

INTG Intention to grant announced

Effective date: 20180605

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE PATENT HAS BEEN GRANTED

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 1056062

Country of ref document: AT

Kind code of ref document: T

Effective date: 20181115

Ref country code: DE

Ref legal event code: R096

Ref document number: 502013011403

Country of ref document: DE

REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: ALDO ROEMPLER PATENTANWALT, CH

REG Reference to a national code

Ref country code: CH

Ref legal event code: PK

Free format text: BERICHTIGUNGEN

RIN2 Information on inventor provided after grant (corrected)

Inventor name: ROESER, TINA

Inventor name: LUTZ, WEBER

REG Reference to a national code

Ref country code: SE

Ref legal event code: TRGR

REG Reference to a national code

Ref country code: NL

Ref legal event code: FP

REG Reference to a national code

Ref country code: DK

Ref legal event code: T3

Effective date: 20190206

REG Reference to a national code

Ref country code: CH

Ref legal event code: PK

Free format text: BERICHTIGUNG B8

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2704424

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20190318

REG Reference to a national code

Ref country code: CH

Ref legal event code: PK

Free format text: BERICHTIGUNGEN

RIN2 Information on inventor provided after grant (corrected)

Inventor name: ROESER, TINA

Inventor name: WEBER, LUTZ

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190124

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190124

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190224

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190125

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20190224

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: RS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

REG Reference to a national code

Ref country code: DE

Ref legal event code: R026

Ref document number: 502013011403

Country of ref document: DE

PLBI Opposition filed

Free format text: ORIGINAL CODE: 0009260

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PLAX Notice of opposition and request to file observation + time limit sent

Free format text: ORIGINAL CODE: EPIDOSNOBS2

26 Opposition filed

Opponent name: MURGITROYD & COMPANY

Effective date: 20190723

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PLAB Opposition data, opponent's data or that of the opponent's representative modified

Free format text: ORIGINAL CODE: 0009299OPPO

R26 Opposition filed (corrected)

Opponent name: MURGITROYD & COMPANY

Effective date: 20190723

PLBB Reply of patent proprietor to notice(s) of opposition received

Free format text: ORIGINAL CODE: EPIDOSNOBS3

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PLAY Examination report in opposition despatched + time limit

Free format text: ORIGINAL CODE: EPIDOSNORE2

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190705

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20190705

PLBC Reply to examination report in opposition received

Free format text: ORIGINAL CODE: EPIDOSNORE3

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20130705

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

PUAH Patent maintained in amended form

Free format text: ORIGINAL CODE: 0009272

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: PATENT MAINTAINED AS AMENDED

27A Patent maintained in amended form

Effective date: 20220209

AK Designated contracting states

Kind code of ref document: B2

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

REG Reference to a national code

Ref country code: DE

Ref legal event code: R102

Ref document number: 502013011403

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: FP

REG Reference to a national code

Ref country code: DE

Ref legal event code: R081

Ref document number: 502013011403

Country of ref document: DE

Owner name: THINXXS MICROTECHNOLOGY GMBH, DE

Free format text: FORMER OWNER: THINXXS MICROTECHNOLOGY AG, 66482 ZWEIBRUECKEN, DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: PD

Owner name: THINXXS MICROTECHNOLOGY GMBH; DE

Free format text: DETAILS ASSIGNMENT: CHANGE OF OWNER(S), CHANGE OF LEGAL ENTITY; FORMER OWNER NAME: THINXXS MICROTECHNOLOGY AG

Effective date: 20220428

REG Reference to a national code

Ref country code: ES

Ref legal event code: PC2A

Owner name: THINXXS MICROTECHNOLOGY GMBH

Effective date: 20220512

REG Reference to a national code

Ref country code: DK

Ref legal event code: T4

Effective date: 20220509

REG Reference to a national code

Ref country code: SE

Ref legal event code: RPEO

REG Reference to a national code

Ref country code: ES

Ref legal event code: DC2A

Ref document number: 2704424

Country of ref document: ES

Kind code of ref document: T5

Effective date: 20220520

REG Reference to a national code

Ref country code: BE

Ref legal event code: PD

Owner name: THINXXS MICROTECHNOLOGY GMBH; DE

Free format text: DETAILS ASSIGNMENT: CHANGE OF OWNER(S), CHANGE OF LEGAL ENTITY; FORMER OWNER NAME: THINXXS MICROTECHNOLOGY AG

Effective date: 20220425

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20181024

REG Reference to a national code

Ref country code: AT

Ref legal event code: HC

Ref document number: 1056062

Country of ref document: AT

Kind code of ref document: T

Owner name: THINXXS MICROTECHNOLOGY GMBH, DE

Effective date: 20220628

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: NL

Payment date: 20230719

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20230724

Year of fee payment: 11

Ref country code: GB

Payment date: 20230720

Year of fee payment: 11

Ref country code: ES

Payment date: 20230926

Year of fee payment: 11

Ref country code: CH

Payment date: 20230801

Year of fee payment: 11

Ref country code: AT

Payment date: 20230720

Year of fee payment: 11

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: SE

Payment date: 20230719

Year of fee payment: 11

Ref country code: FR

Payment date: 20230725

Year of fee payment: 11

Ref country code: DK

Payment date: 20230721

Year of fee payment: 11

Ref country code: DE

Payment date: 20230719

Year of fee payment: 11

Ref country code: BE

Payment date: 20230719

Year of fee payment: 11