EP2697381A1 - Procédé de fermentation à haute efficacité - Google Patents

Procédé de fermentation à haute efficacité

Info

Publication number
EP2697381A1
EP2697381A1 EP12719300.1A EP12719300A EP2697381A1 EP 2697381 A1 EP2697381 A1 EP 2697381A1 EP 12719300 A EP12719300 A EP 12719300A EP 2697381 A1 EP2697381 A1 EP 2697381A1
Authority
EP
European Patent Office
Prior art keywords
fermentation
cells
fermenter
process according
carbohydrates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12719300.1A
Other languages
German (de)
English (en)
Inventor
Vambola Kolbakov
Igor Valerianovich Ilushka
Vladimir Rakitin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Springhill SA
Original Assignee
Springhill SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Springhill SA filed Critical Springhill SA
Priority to EP12719300.1A priority Critical patent/EP2697381A1/fr
Publication of EP2697381A1 publication Critical patent/EP2697381A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Definitions

  • the present invention relates to a technological process for producing fermentation products such as ethanol, butanol, lactic acid, acetic acid and others by novel continuous periodic fermentation.
  • Carbohydrates from several sources cereals, potatoes, cassava, molasses etc), as the substrates, can reprocessed with a very high productivity speed and practically all (up to 92%) of the carbohydrates is converted into desired products.
  • the process is makes the conversion of carbohydrates close to calculated theoretical maximum.
  • periodical fermentation i.e. the adding substrates and removing of products stepwise
  • the adding of secondary culture into fermentation system makes the primary culture more viable, as inducing the competition signals.
  • Such processes are characterized: by low productivity (low production rate of metabolites) and delivery of enormous amounts of waste water, which contain cells of microorganisms, by products, substrate residues etc (i.e. stillage).
  • the amount of waste water can be a little reduced when fed-batch fermentation is used.
  • Fed-batch fermentation is distinguished from batch fermentation by the addition, during operations, of a certain amount of fresh substrate and by the consequent withdrawal of a proportioned amount of broth.
  • the fed-batch fermentation is characterized by a little longer overall time cycle that is certainly acceptable for industrial practice where at present for the effective operating procedures the sterilization after every work cycle, for the preventing of contamination are currently used.
  • One of the key points to increase the rate production of metabolites by fermentation is to maintain the microbial concentration as high as possible.
  • one way of reaching a high concentration of biomass in a fermenter and of decreasing of amounts of waste water, which contain cells of microorganisms is the application of continuous cell-recycle bioreactors, in particular, cell-recycle membrane bioreactors.
  • the purpose of the present invention is carrying out of fermentation process under such conditions at which: high productivity ensured, and practically full conversion of carbohydrates is reached.
  • organic products such as ethanol, butanol, lactic acid, acetic acid and other products can be produced in a simple and inexpensive manner by eliminating the disadvantages of the processes discussed above.
  • the optimal growth rate of cells reach by combination of stirring and filtration speeds, temperature and pH rates, and by amount of needed amounts of nutrients and bacteriostatics fed into the fermenter.
  • each periodic stage of production of organic products by fermentation comprises three steps: 1) loading a water solution of carbohydrates, nutrients, bacteriostatics, salts and cells of microorganisms to fermenter, concentration of cultures yeast or bacterial strain in a fermenter is maintained approximately on fixed state between 5-100 grams dry cell per litre (preferably 20-100 grams dry cell per litre); 2) fermentation with formation metabolites (organic products); 3) removal from fermenter a cell-free water solution metabolites, with re-direction to fermenter the concentrated in filter unit suspension of cells of microorganisms.
  • process containing with the concentrated suspension of cells (remained after the third stage), load a fresh portion of a water solution of carbohydrates, and if it is necessary, nutrients, bacteriostatics and salts, then repeat above operations (steps 2 and 3).
  • the amount of the concentrated suspension of cells removed from a fermenter are essentially less in comparison with a stream of cells at continuous realization a fermentation (so-called “cell bleed”), not to mentioning about conventional periodic process of a batch-fermentation.
  • a fermenter connected with a cell-recycle device can be used.
  • a cell-recycle device is a membrane module.
  • the given process allows to produce organic products with high productivity (up to 200 g/L/h) and in this conditions the amount of waste water, which contain cells of microorganisms, does not exceed values of 10 gram per 1 kg of a target product.
  • the membrane recycle bioreactor system consists of the fermenter (10 L glass vessel) equipped with pH controller, controller of temperature and agitation.
  • Step 1 At the beginning fermenter is load 5.7 l by mixture of the following composition, g/l: Carbohydrates 30-200 Nutrients 3-6 Salts of K, Na, Mg and other 10-15 Bacteriostatics (optional) 1-6 (mg/l) and suspension of cells of microorganisms for creation of concentration of cells in fermenter, equal to 5-100 g/l (counting upon dry-cells).
  • Step 2 The process of fermentation is carried out up to practically full conversion of carbohydrates (>99.9%), automatically controlling temperature (temperature range of 37-55 °C) and oul ⁇ (pH range of 5-7) in fermenter by the addition of 6-12% NH 4 OH.
  • temperature temperature range of 37-55 °C
  • oul ⁇ pH range of 5-7
  • a separation process is begins.
  • the minimal cells growth rate of cultures yeast or bacterial strain in bioreactor which is sufficient to provide cells death replacement is controlled by a choice of the certain temperature, pH and amount of nutrients feeding into the fermenter and by using of bacteriostatics.
  • Step 3 The process of separation is carried out by passing under pressure the resulted broth via membrane module, collecting a water solution free of cell products and returning the concentrated suspension of cells to fermenter.
  • Process of separation stop when in a fermenter there will be approximately 10-15 % of a broth from its initial volume. The remained broth is concentrated suspension of cells of microorganisms.
  • Glucose, lactose, fructose, galactose, sucrose can be used as carbohydrates.
  • nutrients peptone protein extracts and yeast extract can be used.
  • salt K 2 HPO 4 , KH 2 PO 4 , NaCl, Na acetate, MgSO 4 , Fe 2 (SO 4 ) 3 , ZnSO 4 , MnSO 4 and others can be used.
  • Ammonia used for the neutralization of fermentation environment utilized by the bacterium as the nitrogen source thus fewer nutrients needed. This makes the product cheaper and filtrate solution clearer. The last point makes the separation process easier, what means cheaper.
  • the fermentation method of the present invention can be applied not only to produce of lactic acid and ethanol, but also to the other organic acids, alcohols and esters, such as acetic acid, formic acid, propionic acid, citric acid, malic acid, maleic acid, malonic acid, fumaric acid, succinic acid, butanol, ethyl acetate and others.
  • acetic acid formic acid, propionic acid, citric acid, malic acid, maleic acid, malonic acid, fumaric acid, succinic acid, butanol, ethyl acetate and others.
  • T the temperature of fermentation, °C; C C 1,I – the initial concentration of cells of microorganisms in fermenter at the first stage of fermentation, gram/Litre (dry cell); C C 1,E – the concentration of cells of microorganisms in fermenter after the first stage of fermentation, gram/Litre (dry cell); C C 10,E – the concentration of cells of microorganisms in fermenter after the tenth stage of fermentation, gram/Litre (dry cell); C CH I – the initial concentration of carbohydrates in fermenter at all stages of fermentation, gram/Litre; C N 0 – the concentration of nutrients in fermenter at the first stage of fermentation, gram/Litre; C S – the concentration of salts in fermenter at all stages of fermentation, g/L; C B – the concentration of bacteriostatics in fermenter at all stages of fermentation, mg/L; C S – the concentration of neutralizator in the inlet, M; C E – the concentration

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Cette invention concerne un procédé périodique continu de production de substances organiques telles que l'éthanol, le butanol, l'acide lactique, l'acide acétique et autres produits chimiques de base destinés à former d'autres dérivés. Pour la fermentation, on utilise des matériaux contenant des glucides, en présence de cultures de souches de levures ou bactériennes. La fermentation est mise en œuvre dans des conditions induisant une vitesse de croissance minimale des cellules en bioréacteur et permet d'obtenir une conversion quasi complète des glucides.
EP12719300.1A 2011-04-13 2012-04-13 Procédé de fermentation à haute efficacité Withdrawn EP2697381A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP12719300.1A EP2697381A1 (fr) 2011-04-13 2012-04-13 Procédé de fermentation à haute efficacité

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP11162196A EP2511375A1 (fr) 2011-04-13 2011-04-13 Procédé de fermentation à haut rendement
PCT/EP2012/056821 WO2012140223A1 (fr) 2011-04-13 2012-04-13 Procédé de fermentation à haute efficacité
EP12719300.1A EP2697381A1 (fr) 2011-04-13 2012-04-13 Procédé de fermentation à haute efficacité

Publications (1)

Publication Number Publication Date
EP2697381A1 true EP2697381A1 (fr) 2014-02-19

Family

ID=44318130

Family Applications (2)

Application Number Title Priority Date Filing Date
EP11162196A Withdrawn EP2511375A1 (fr) 2011-04-13 2011-04-13 Procédé de fermentation à haut rendement
EP12719300.1A Withdrawn EP2697381A1 (fr) 2011-04-13 2012-04-13 Procédé de fermentation à haute efficacité

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP11162196A Withdrawn EP2511375A1 (fr) 2011-04-13 2011-04-13 Procédé de fermentation à haut rendement

Country Status (3)

Country Link
EP (2) EP2511375A1 (fr)
EA (1) EA201301163A1 (fr)
WO (1) WO2012140223A1 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103011504B (zh) * 2012-12-10 2013-12-11 甘肃汇能生物工程有限公司 一种那西肽工业生产中发酵废水循环利用方法
RU2612152C2 (ru) * 2013-12-12 2017-03-02 Общество с ограниченной ответственностью "БиоМИХМ" Способ получения молочной кислоты
CN104774734B (zh) * 2015-04-09 2016-08-17 福建师范大学 一种富含γ-氨基丁酸的红曲醋制备方法
CN105044163A (zh) * 2015-09-09 2015-11-11 江南大学 一种微生物发酵乙醇浓度在线检测装置和方法
RU2665842C1 (ru) * 2017-12-05 2018-09-04 Общество с ограниченной ответственностью ООО "Инжиниринговый центр "Зеленая химия" Способ получения молочной кислоты

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Publication number Priority date Publication date Assignee Title
EE200700037A (et) * 2007-07-10 2009-02-16 Nordbiochem OÜ Meetod orgaaniliste hapete tootmiseks pideva k„„ritamisega

Non-Patent Citations (1)

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Title
See references of WO2012140223A1 *

Also Published As

Publication number Publication date
EP2511375A1 (fr) 2012-10-17
WO2012140223A1 (fr) 2012-10-18
EA201301163A1 (ru) 2014-06-30

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