EP2612145A2 - Zellbasierter test zur messung der biologischen aktivität von anti-tshr-autoimmunantikörpern - Google Patents
Zellbasierter test zur messung der biologischen aktivität von anti-tshr-autoimmunantikörpernInfo
- Publication number
- EP2612145A2 EP2612145A2 EP11751888.6A EP11751888A EP2612145A2 EP 2612145 A2 EP2612145 A2 EP 2612145A2 EP 11751888 A EP11751888 A EP 11751888A EP 2612145 A2 EP2612145 A2 EP 2612145A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- tshr
- cells
- bioassay
- signal
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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- 230000004071 biological effect Effects 0.000 title claims abstract description 13
- 101150098159 TSHR gene Proteins 0.000 title claims description 3
- 238000005259 measurement Methods 0.000 title description 14
- 238000000423 cell based assay Methods 0.000 title description 2
- 102000003911 Thyrotropin Receptors Human genes 0.000 claims abstract description 105
- 108090000253 Thyrotropin Receptors Proteins 0.000 claims abstract description 105
- 238000004166 bioassay Methods 0.000 claims abstract description 52
- 239000013610 patient sample Substances 0.000 claims abstract description 42
- 239000000523 sample Substances 0.000 claims abstract description 40
- 230000004936 stimulating effect Effects 0.000 claims abstract description 35
- 230000000903 blocking effect Effects 0.000 claims abstract description 31
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- 102000011923 Thyrotropin Human genes 0.000 claims 4
- 108010061174 Thyrotropin Proteins 0.000 claims 4
- 239000004017 serum-free culture medium Substances 0.000 claims 1
- 238000003556 assay Methods 0.000 description 33
- 210000002966 serum Anatomy 0.000 description 18
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- 238000008940 Alkaline Phosphatase assay kit Methods 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
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- 102100024321 Alkaline phosphatase, placental type Human genes 0.000 description 1
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- 239000012894 fetal calf serum Substances 0.000 description 1
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- 238000010438 heat treatment Methods 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 208000003532 hypothyroidism Diseases 0.000 description 1
- 230000002989 hypothyroidism Effects 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000000099 in vitro assay Methods 0.000 description 1
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- 108010031345 placental alkaline phosphatase Proteins 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
- G01N2500/10—Screening for compounds of potential therapeutic value involving cells
Definitions
- the problem underlying the invention is the provision of a precise, easy-to-perform and cost-efficient method to charac ⁇ terize and quantify the biological activity of anti TSHR autoimmune antibodies in patient samples .
- Fig. 3 shows the reproducibility of the bioassay.
- a "patient sample” according to the invention comprises at least blood serum or plasma from a human who may have Graves' disease (hyperthyroidism) or hypothyroidism.
- the SU values measured in all sample / cell line lot combinations are shown as data in the diagram of Fig. 2.
- the results demonstrate an intra-assay variance and an inter-assay variability below 10% over the whole range of measured SU values. This means that the assay produces accurate reproducible SU values for patient sera (samples B, C) and plasma (sample D) .
- Sample E further shows that even at higher concentrations of TSHR stimulating agent (in. this case TSH) , the assay leads to highly reproducible results .
- Example 5 Comparison of the clinical score of patients with the SU values of samples from the same patients determined by the bioassay according to the invention.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Endocrinology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Reproductive Health (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11751888.6A EP2612145A2 (de) | 2010-08-31 | 2011-08-31 | Zellbasierter test zur messung der biologischen aktivität von anti-tshr-autoimmunantikörpern |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10009042 | 2010-08-31 | ||
| EP11751888.6A EP2612145A2 (de) | 2010-08-31 | 2011-08-31 | Zellbasierter test zur messung der biologischen aktivität von anti-tshr-autoimmunantikörpern |
| PCT/EP2011/065035 WO2012028654A2 (en) | 2010-08-31 | 2011-08-31 | Cell based assay for measurement of biological activity of anti tshr autoimmune antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP2612145A2 true EP2612145A2 (de) | 2013-07-10 |
Family
ID=44545728
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP11751888.6A Withdrawn EP2612145A2 (de) | 2010-08-31 | 2011-08-31 | Zellbasierter test zur messung der biologischen aktivität von anti-tshr-autoimmunantikörpern |
Country Status (2)
| Country | Link |
|---|---|
| EP (1) | EP2612145A2 (de) |
| WO (1) | WO2012028654A2 (de) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107271680B (zh) * | 2017-05-22 | 2019-04-23 | 中山大学附属第三医院 | 人髓鞘少突胶质细胞糖蛋白自身免疫抗体的检测方法 |
| WO2020050208A1 (ja) * | 2018-09-05 | 2020-03-12 | ヤマサ醤油株式会社 | Ｔｓｈ受容体に対する自己抗体活性の迅速測定方法及びキット |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0327960A1 (de) | 1988-02-11 | 1989-08-16 | F. Hoffmann-La Roche Ag | Sekretierbare Formen von alkalischer Phosphatase |
| GB9720693D0 (en) * | 1997-09-30 | 1997-11-26 | Univ Wales Medicine | Assay |
-
2011
- 2011-08-31 EP EP11751888.6A patent/EP2612145A2/de not_active Withdrawn
- 2011-08-31 WO PCT/EP2011/065035 patent/WO2012028654A2/en not_active Ceased
Non-Patent Citations (1)
| Title |
|---|
| See references of WO2012028654A2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012028654A2 (en) | 2012-03-08 |
| WO2012028654A3 (en) | 2015-07-02 |
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Legal Events
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| DAX | Request for extension of the european patent (deleted) | ||
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
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| 18D | Application deemed to be withdrawn |
Effective date: 20140301 |
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| R17D | Deferred search report published (corrected) |
Effective date: 20150702 |