WO2012028654A3 - Cell based assay for measurement of biological activity of anti tshr autoimmune antibodies - Google Patents

Cell based assay for measurement of biological activity of anti tshr autoimmune antibodies Download PDF

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Publication number
WO2012028654A3
WO2012028654A3 PCT/EP2011/065035 EP2011065035W WO2012028654A3 WO 2012028654 A3 WO2012028654 A3 WO 2012028654A3 EP 2011065035 W EP2011065035 W EP 2011065035W WO 2012028654 A3 WO2012028654 A3 WO 2012028654A3
Authority
WO
WIPO (PCT)
Prior art keywords
tshr
cells
signal
biological activity
autoimmune antibodies
Prior art date
Application number
PCT/EP2011/065035
Other languages
French (fr)
Other versions
WO2012028654A2 (en
Inventor
Ulrich Loos
Dimitrij Zeiger
Original Assignee
Krelo Gmbh Medical Diagnostics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Krelo Gmbh Medical Diagnostics filed Critical Krelo Gmbh Medical Diagnostics
Priority to EP11751888.6A priority Critical patent/EP2612145A2/en
Publication of WO2012028654A2 publication Critical patent/WO2012028654A2/en
Publication of WO2012028654A3 publication Critical patent/WO2012028654A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Endocrinology (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Reproductive Health (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a bioassay for the determination of biological activity of stimulating autoimmune antibodies directed against the TSHR receptor comprising the steps of a) incubating TSHR expressing cells with a patient sample, b) detecting the intensity of a signal in the supernatant of the cells that is caused by a change of cAMP concentration in the cells resulting from TSHR stimulation, c) determining the stimulating activity of the anti TSHR autoimmune antibodies by comparing the signal intensity of step b) with the signal intensity obtained after incubation of a first reference sample with TSHR expressing cells; the invention also refers to a bioassay for the determination of the biological activity of blocking autoimmune antibodies directed against the thyroid stimulating hormone receptor (TSHR), comprising the steps of a) incubating TSHR expressing cells with a patient sample, b) detecting the intensity of a signal in the supernatant of the cells that is caused by a change of cAMP concentration in the cells resulting from TSHR stimulation, c) determining the blocking/stimulation activity of the anti TSHR auto antibodies by comparing the signal intensity of step b) with the signal intensities obtained after incubation of a first and a second reference sample with TSHR expressing cells, wherein the incubation sample of step a) and the second reference sample contain the same amount of a TSHR stimulating agent.
PCT/EP2011/065035 2010-08-31 2011-08-31 Cell based assay for measurement of biological activity of anti tshr autoimmune antibodies WO2012028654A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11751888.6A EP2612145A2 (en) 2010-08-31 2011-08-31 Cell based assay for measurement of biological activity of anti tshr autoimmune antibodies

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10009042 2010-08-31
EP10009042.2 2010-08-31

Publications (2)

Publication Number Publication Date
WO2012028654A2 WO2012028654A2 (en) 2012-03-08
WO2012028654A3 true WO2012028654A3 (en) 2015-07-02

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2011/065035 WO2012028654A2 (en) 2010-08-31 2011-08-31 Cell based assay for measurement of biological activity of anti tshr autoimmune antibodies

Country Status (2)

Country Link
EP (1) EP2612145A2 (en)
WO (1) WO2012028654A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107271680B (en) * 2017-05-22 2019-04-23 中山大学附属第三医院 The detection method of human myelin oligodendroglia glycoprotein autoimmune antibody
US20210318305A1 (en) * 2018-09-05 2021-10-14 Yamasa Corporation Method and Kit for Rapid Measurement of Autoantibody Activity with Respect to TSH Receptor

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999016902A1 (en) * 1997-09-30 1999-04-08 University Of Wales College Of Medicine Bioassay for thyroid stimulating antibodies

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0327960A1 (en) 1988-02-11 1989-08-16 F. Hoffmann-La Roche Ag Secretable forms of alkaline phosphatase

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999016902A1 (en) * 1997-09-30 1999-04-08 University Of Wales College Of Medicine Bioassay for thyroid stimulating antibodies

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANONYMOUS: "Pathway Profiling SystemUser Manual", 23 February 2009 (2009-02-23), XP002665967, Retrieved from the Internet <URL:http://www.clontech.com/xxclt_ibcGetAttachment.jsp?cItemId=17526> [retrieved on 20111216] *
LYTTON S D ET AL: "Bioassays for TSH-receptor autoantibodies: An update", AUTOIMMUNITY REVIEWS, ELSEVIER, AMSTERDAM, NL, vol. 10, no. 2, 1 December 2010 (2010-12-01), pages 116 - 122, XP027506586, ISSN: 1568-9972, [retrieved on 20101118], DOI: 10.1016/J.AUTREV.2010.08.018 *
S. D. LYTTON ET AL: "A Novel Thyroid Stimulating Immunoglobulin Bioassay Is a Functional Indicator of Activity and Severity of Graves' Orbitopathy", JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, vol. 95, no. 5, 1 May 2010 (2010-05-01), pages 2123 - 2131, XP055015052, ISSN: 0021-972X, DOI: 10.1210/jc.2009-2470 *
WATSON P F ET AL: "A new chemiluminescent assay for the rapid detection of thyroid stimulating antibodies in Graves' disease", CLINICAL ENDOCRINOLOGY, BLACKWELL SCIENTIFIC PUBLICATIONS, OXFORD, GB, vol. 49, 1 November 1998 (1998-11-01), pages 577 - 581, XP002089745, ISSN: 0300-0664, DOI: 10.1046/J.1365-2265.1998.00619.X *

Also Published As

Publication number Publication date
WO2012028654A2 (en) 2012-03-08
EP2612145A2 (en) 2013-07-10

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