EP2563900A2 - Tanks for culturing photosynthetic organisms - Google Patents

Tanks for culturing photosynthetic organisms

Info

Publication number
EP2563900A2
EP2563900A2 EP11723192A EP11723192A EP2563900A2 EP 2563900 A2 EP2563900 A2 EP 2563900A2 EP 11723192 A EP11723192 A EP 11723192A EP 11723192 A EP11723192 A EP 11723192A EP 2563900 A2 EP2563900 A2 EP 2563900A2
Authority
EP
European Patent Office
Prior art keywords
basins
basin
gutter
cover
length
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP11723192A
Other languages
German (de)
French (fr)
Inventor
Dominique Delobel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ALGAESTREAM
Original Assignee
ALGAESTREAM
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ALGAESTREAM filed Critical ALGAESTREAM
Publication of EP2563900A2 publication Critical patent/EP2563900A2/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/18—Open ponds; Greenhouse type or underground installations
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means

Definitions

  • the present invention describes a covered basin, advantageously (but not exclusively) intended for the equipment of terrestrial aquaculture farms of photosynthetic organisms and the technical device necessary for its production.
  • the field of the invention therefore relates to covered basins of all sizes, particularly those with a unit size equal to or greater than 500 square meters.
  • a terrestrial aquaculture farm of photosynthetic organisms consists of a set of basins often of identical shape but in several series of sizes. Efficiency leads to the arrangement of the basins in order to optimize the occupation of the ground, which most often results in the alignment of identical basins on one or more rows.
  • Each basin is separated from its immediate neighbor by a space which allows the free movement of operating personnel and equipment and which can serve as a support for the networks of the installation such as channels, pipes, cables.
  • the basins are shallow to ensure the greatest diffusion of sunlight throughout the liquid medium and are most often built or placed above the ground.
  • the walls of said basins are generally made of concrete walls, solid or hollow bricks, planks, earth embankment or packed sand, or any other suitable material or device.
  • the low height of the contained liquid usually limited to a few tens of centimeters causes a low side pressure on the walls, such as less than 5000 pascals for a liquid height of 50 centimeters, which allows the use of thin walls or flexible implemented in accordance with the rules of art.
  • each basin has its own cover which is supported either on the same wall of the basin which in this case must be reinforced and adapted to support this load, the more often outdoors to include a taxiway around the entire perimeter of the pond inside the cover.
  • the scope of the structure is at least equal to the width of the pool possibly increased by the width of the lateral traffic lanes.
  • b) collective: the same coverage covers several basins.
  • the ground anchors of the support structure of this cover are regularly spaced according to the rules of the art and positioned in the spaces between the basins where they constitute obstacles to traffic.
  • the scope of the structure is at least equal to the width of a single basin increased by the width of a taxiway between two basins.
  • the covers provide among other functions, that of taking care of precipitation, especially rains.
  • Rainwater is systematically conduct at the periphery of the cover, at one or more points depending on the shape of the support structure of said cover, and according to the forms and accessories adapted to each particular embodiment.
  • the rainwater is brought to the ground in the space normally devolved to the circulation between the covers (individual or collective) and must be supported by any appropriate means such as, for example, channel, pipe, gutter , to be evacuated outside the zone of basins, with the aid of equipment or arrangements known to those skilled in the art and according to the needs due to the relief of the site as ditches, catch basins, lift pumps, basin retention.
  • any appropriate means such as, for example, channel, pipe, gutter , to be evacuated outside the zone of basins, with the aid of equipment or arrangements known to those skilled in the art and according to the needs due to the relief of the site as ditches, catch basins, lift pumps, basin retention.
  • the invention overcomes some of these disadvantages and helps to facilitate the deployment of pools and reduce costs by saving materials. At least.
  • the invention is based on the placing on the ground of a set of gutters of great length, advantageously regularly spaced, so as to allow the installation of basins between the gutters, said gutters can ensure both the wall function basins, support of the cover of said basins, taxiway for operating personnel, support networks, collection and management of rainwater outside the basin area.
  • a first object of the invention is a device for constructing covered basement walls composed of a profiled trough ⁇ of great length, advantageously placed on the ground, which can act as a wall, advantageously a wall for two juxtaposed basins, and of which the profile can be used to fix the bottom and bottom films of the basins® for the entire length of the gutter.
  • the invention also relates to a covered basin, characterized by the implementation of at least one profile gutter ⁇ of great length as a side wall, preferably common to two juxtaposed basins, and as a support for the upper cover flexible airtight and water-resistant of each basin combined with the use of films or flexible sheets as bas fonds.
  • Figure 1 shows a view of the transverse profile of a particular embodiment of the gutter.
  • FIG. 2 represents a view from above of a set of basins made using the gutter
  • FIG. 3 represents a cross-sectional view along AA of FIG. 2.
  • Figure 4 shows a top view of a set of basins made using the gutter (variant of Figure 2).
  • the gutter ⁇ , essential device of the invention can take the usual form known to those skilled in the art in its traditional use.
  • said gutter ⁇ may be of very great length up to several hundred meters, advantageously of 5 to 500 meters, even more preferably from 10 to 300 meters.
  • Said gutter ⁇ may have a substantially U-shaped transverse profile with a bottom and side walls, advantageously such as that described in FIG. 1.
  • the bottom ⁇ which may be placed directly on the ground, may be a few tens of centimeters wide. several meters, for example from 10 centimeters to 10 meters, advantageously from 30 centimeters to 3 meters, even more advantageously from 40 to 100 centimeters.
  • the side walls ⁇ preferably vertical, may be 5 to 100 centimeters high, preferably 10 to 30 centimeters may have at their upper end a fold ⁇ which can run the entire length of the gutter.
  • the longitudinal fold may be intended to i) provide the traditional function of stiffening the wall ⁇ to limit the deformation ii) allow the attachment of flexible films bottom and cover pools (see below).
  • this fold may have a rounded shape or have sharp edges, be oriented outwardly or inwardly of the gutter.
  • said fold ⁇ can be split.
  • a first fold can be positioned at the ridge of the side wall ⁇ and can ensure the stiffening.
  • a second fold can be positioned between the ridge and the base of the side wall ⁇ and can provide the function of hanging flexible films (see below).
  • Said gutter necessary for carrying out the invention may be manufactured by any appropriate technical means known to those skilled in the art, as non-limiting examples:
  • thermoplastic material in sections of a few meters assembled by gluing, welding, bolting or riveting to obtain the required long length (PVC tube or pipe production technique).
  • said gutters can be simply placed on a floor, possibly pre-leveled and if necessary compacted according to the appropriate techniques known to those skilled in the art.
  • ii) achieve a nearly perfect planimetry so that any two points of the entire support surface defined in (i) have a maximum altitude difference of less than or equal to 5 centimeters and ideally less than or equal to 2 centimeters, tolerance level widely available to specialized companies for example in the realization of road surfaces.
  • iii) obtain a surface layer freed from any element likely to attack the soft materials that can be deployed to form the bottoms of the basins®.
  • the establishment of a fine sand setting layer after stone removal is an example of a solution to achieve this objective.
  • the gutters ⁇ can be placed on the support surface thus produced, in the following ways:
  • all gutters can be ideally made identical, regardless of the particular embodiment, and can have at least a single transverse profile (same design, same dimensions).
  • the gutters may be laid strictly parallel.
  • the gap between the gutters can determine the width of the basins
  • the gutters may be of substantially identical length. This length can determine that of basins which generally is slightly lower.
  • the gutters can be firmly held in place by any means appropriate to the particular embodiment such as for example 1) by the own weight of the gutter made of concrete 2) by partial filling by any suitable material such as sand, water, gravel , concrete or other, 3) by crampons fixed in the ground by any suitable means through the bottom CD or tangentially to the walls ⁇ .
  • the bottom of the basins can be made using a waterproof flexible film ® whose width is equal to the spacing between two gutters plus double the height of the vertical wall ⁇ said gutter.
  • the length of said flexible film must be equal to that of the gutters.
  • said flexible film ® may be in any material compatible with the intended function, such as for example and without limitation the ethylene-propylene-diene monomer membranes (EPDM), polyvinyl chloride or polyvinyl chloride (PVC) ), polyethylene film (PE).
  • the rules of the art of laying down the bottom film may require the prior installation of a protective layer of a suitable material such as for example a geotextile felt.
  • the bottom of the basin may consist of the superposition of several flexible films.
  • the pond cover ® can be provided by a film that is air and water tight and permeable to visible light.
  • a polyethylene film commonly used in agriculture can meet these characteristics.
  • the width of the cover film may be substantially equal to that of the pelvis bottom film.
  • Each of the two fund and cover films of the pool can be unrolled and laid stretched and unfolded on the ground and over the entire basin area.
  • the longitudinal edges of the two superimposed films can be brought together against the upper edge of the same side of the side gutter to be fixed by any means adapted to the particular embodiment of the entire length of the gutter, continuously.
  • This attachment can be made by way of example, by a rubber bead compressing the two films inside the ply®, or by any suitable system or device capable of providing:
  • the walls of the ends must ensure the maintenance of the bottom film in the high position so as to ensure the tightness of the basin and the necessary retention of the liquid contained.
  • the gutter may be the only rigid structure along the entire length of the basin
  • the maintenance in the high position of the cover can be ensured by placing overpressurized air or gas or gases contained in said basin.
  • This overpressure can be obtained by any suitable device known to those skilled in the art such as a fan, a compressor or a fan.
  • the basin cover may be mobile and at variable altitude, and may, depending on the needs of the operation be kept in the normal up position or in the low position in contact with the liquid contained in the basin simply by stopping the blower devices.
  • This arrangement makes it possible to make the basins safe i) by limiting the wind catchment of the basins to a minimum ii) by submerging the cover: the height of the liquid in the basin is necessarily lower than that of the walls ⁇ of the gutters. It is possible to quickly spread over the cover film (airtight and watertight) the amount of water sufficient to cover a few centimeters the entire area of the basin. This operation makes it possible to present all the basins as a liquid space and thus to face very violent winds likely to damage or destroy the installations and in particular the covers of the basins if they were kept in the high position. Overpressurisation of the interior of the basin may allow the resumption of normal operation of the installation: the swelling of the cover and its elevation from close to hunting flush the upper layer of water flowing to the side gutters and can be evacuated according to the normal mode of operation.
  • the stiffener has two functions: i) to strictly maintain the spacing of the walls ⁇ of the gutter to the width of the bottom, ii) to compress the rim (D of the gutter to control the opening and secure the fixing of the films (D and ® .
  • the evacuation of water by the only ends of the gutters may be insufficient depending on the section. transverse of the gutter.
  • intermediate evacuations ⁇ advantageously regularly spaced along the length of the gutters in relation to the rainfall regime of the particular geographical area of the installation (for example every fifty meters).
  • Said evacuations may be connected to an underground pipe, advantageously disposed perpendicularly, to the gutters.
  • Each underground pipe can be made of materials and dimensions adapted to the rules of art, and can be installed prior to the installation of the gutters ⁇ and the films of basin ( D and can end on both sides of the basin area it serves to a ditch ® or main pipe whose function is to bring collected water out of the basin area.
  • a singlecollectau serves as wall to two contiguous basins, support structure to the cover of said basins, and means of collection and disposal of rainwater, ii) rapidity of installation of basins iii) optimization of land use by drastically reducing the space between basins iiii) reduced wind intake limiting the height of the cover to less than one quarter of the width of the pool.
  • the invention is particularly intended for the equipment of large scale microalgae and photosynthetic culture farms, in particular such that they may result from the implementation of the invention which is the subject of French patent no. 2,918,387.
  • the subject of the invention is also a device for constructing covered pool walls according to the invention, composed of a long profiled gutter placed on the ground, which acts as a wall for two juxtaposed basins and whose profile allows the fixing along the entire length of the basement films and basins.
  • the invention also relates to the use of tanks according to the invention in aquaculture farms and said aquaculture farms comprising at least one basin according to the invention.

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Environmental & Geological Engineering (AREA)
  • Sewage (AREA)
  • Cultivation Of Seaweed (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a method for constructing covered tanks for terrestrial agriculture of photosynthetic organisms and to the associated device made up of a very long gutter which acts simultaneously as a wall common to two juxtaposed tanks, as a support for the cover of the tanks, as a walkway for operating personnel and as a receptacle for rainwater. The invention is particularly suitable for the equipment of large farms dedicated to the production of algae or other photosynthetic organisms.

Description

Bassins de culture d'organismes photosynthétiques  Pools of photosynthetic organisms
La présente invention décrit un bassin couvert, avantageusement (mais pas exclusivement) destiné à l'équipement de fermes d'aquaculture terrestre d'organismes photosynthétiques et le dispositif technique nécessaire pour sa réalisation. The present invention describes a covered basin, advantageously (but not exclusively) intended for the equipment of terrestrial aquaculture farms of photosynthetic organisms and the technical device necessary for its production.
Le domaine de l'invention concerne donc les bassins couverts de toutes tailles, particulièrement ceux d'une taille unitaire égale ou supérieure à 500 mètres carrés.  The field of the invention therefore relates to covered basins of all sizes, particularly those with a unit size equal to or greater than 500 square meters.
Une ferme d'aquaculture terrestre d'organismes photosynthétiques est constituée d'un ensemble de bassins souvent de forme identique mais en plusieurs séries de tailles. L'efficacité conduit à disposer les bassins de manière à optimiser l'occupation du terrain, ce qui abouti le plus souvent à un alignement de bassins identiques sur une ou plusieurs rangées.  A terrestrial aquaculture farm of photosynthetic organisms consists of a set of basins often of identical shape but in several series of sizes. Efficiency leads to the arrangement of the basins in order to optimize the occupation of the ground, which most often results in the alignment of identical basins on one or more rows.
Chaque bassin est séparé de son voisin immédiat par un espace qui permet la libre circulation du personnel d'exploitation et des matériels et qui peut servir de ^support aux réseaux de l'installation tels que canaux, tuyaux, câbles.  Each basin is separated from its immediate neighbor by a space which allows the free movement of operating personnel and equipment and which can serve as a support for the networks of the installation such as channels, pipes, cables.
Les bassins sont de faible profondeur pour assurer la plus grande diffusion de la lumière solaire dans l'ensemble du milieu liquide et sont le plus souvent construits ou posés au dessus du sol.  The basins are shallow to ensure the greatest diffusion of sunlight throughout the liquid medium and are most often built or placed above the ground.
Les parois des dits bassins sont généralement constituées de murets de béton, de briques pleines ou creuses, de planches, d'un remblai de terre ou de sable tassé, ou de tout autre matériau ou dispositif adapté.  The walls of said basins are generally made of concrete walls, solid or hollow bricks, planks, earth embankment or packed sand, or any other suitable material or device.
La faible hauteur du liquide contenu, le plus souvent limitée à quelques dizaines de centimètres entraîne une faible pression latérale sur les parois, comme par exemple moins de 5000 Pascals pour une hauteur de liquide de 50 centimètres, ce qui autorise l'usage de parois minces ou souples mises en œuvre dans le respect des règles de l'art.  The low height of the contained liquid, usually limited to a few tens of centimeters causes a low side pressure on the walls, such as less than 5000 pascals for a liquid height of 50 centimeters, which allows the use of thin walls or flexible implemented in accordance with the rules of art.
La couverture des bassins, quelque soit le mode particulier de réalisation est a) individuelle : chaque bassin dispose de sa propre couverture qui prend appui soit sur la paroi même du bassin qui dans ce cas doit être renforcée et adaptée pour soutenir cette charge, soit le plus souvent à l'extérieur pour inclure une voie de circulation sur tout le périmètre du bassin à l'intérieur de la couverture. La portée de la structure est au minimum égale à la largeur du bassin éventuellement augmentée de la largeur des voies de circulation latérales.  The coverage of the basins, whatever the particular embodiment is a) individual: each basin has its own cover which is supported either on the same wall of the basin which in this case must be reinforced and adapted to support this load, the more often outdoors to include a taxiway around the entire perimeter of the pond inside the cover. The scope of the structure is at least equal to the width of the pool possibly increased by the width of the lateral traffic lanes.
b) collective : une même couverture englobe plusieurs bassins. Les ancrages au sol de la structure support de cette couverture sont espacés régulièrement selon les règles de l'art et positionnés dans les espaces entre les bassins où ils constituent des obstacles à la circulation. La portée de la structure est au minimum égale à la largeur d'un seul bassin augmentée de la largeur d'une voie de circulation entre deux bassins.  b) collective: the same coverage covers several basins. The ground anchors of the support structure of this cover are regularly spaced according to the rules of the art and positioned in the spaces between the basins where they constitute obstacles to traffic. The scope of the structure is at least equal to the width of a single basin increased by the width of a taxiway between two basins.
Ces couvertures prennent souvent la forme de serres tunnels ou serres chapelles connues de l'homme de l'art et dont la hauteur est de quelques mètres généralement entre 3 et 5, ce qui induit une prise au vent importante.  These covers often take the form of greenhouses tunnels or greenhouses chapels known to those skilled in the art and whose height is a few meters generally between 3 and 5, which induces a significant wind gain.
Les couvertures assurent entre autres fonctions, celle de prise en charge des précipitations, en particulier des pluies. L'eau de pluie est systématiquement conduite à la périphérie de la couverture, en un ou plusieurs points selon la forme de la structure de soutien de la dite couverture, et selon les formes et accessoires adaptés a chaque mode particulier de réalisation. The covers provide among other functions, that of taking care of precipitation, especially rains. Rainwater is systematically conduct at the periphery of the cover, at one or more points depending on the shape of the support structure of said cover, and according to the forms and accessories adapted to each particular embodiment.
L'eau de pluie est amenée au sol dans l'espace normalement dévolu à la circulation entre les couvertures (individuelles ou collectives) et doit être prise en charge par tout moyen approprié tel que, à titre d'exemple, canal, tuyau, caniveau, pour être évacué a l'extérieur de la zone des bassins, a l'aide des équipements ou aménagements connus de l'homme de l'art et selon les nécessités dues au relief du site comme fossés, puisards, pompes de relevage, bassin de rétention.  The rainwater is brought to the ground in the space normally devolved to the circulation between the covers (individual or collective) and must be supported by any appropriate means such as, for example, channel, pipe, gutter , to be evacuated outside the zone of basins, with the aid of equipment or arrangements known to those skilled in the art and according to the needs due to the relief of the site as ditches, catch basins, lift pumps, basin retention.
Les chutes de pluie sur une superficie d'un hectare de bassins couverts représente un volume de cent mètres cubes par tranche de dix millimètres de précipitation. L'évacuation de telles quantités est obligatoire sous peine de dégâts comme l'inondation des installations, le déplacement ou la destruction des parois des bassins, le ravinement ou la déstabilisation des sols.  Rainfall over an area of one hectare of covered basins represents a volume of one hundred cubic meters per ten millimeters of precipitation. The evacuation of such quantities is obligatory under pain of damage such as the flooding of the installations, the displacement or the destruction of the walls of the basins, the gully or the destabilization of the grounds.
L'invention remédie à certains de ces inconvénients et contribue à faciliter le déploiement des bassins et à en réduire les coûts par économie de matériaux.au moins.  The invention overcomes some of these disadvantages and helps to facilitate the deployment of pools and reduce costs by saving materials. At least.
L'invention repose sur la mise en place au sol d'un ensemble de chéneaux de grande longueur, avantageusement régulièrement espacés, de manière à permettre l'installation des bassins entre les chéneaux, les dits chéneaux pouvant assurer à la fois la fonction de paroi des bassins, de support de la couverture des dits bassins, de voie de circulation pour le personnel d'exploitation, de support aux réseaux, de collecte et de conduite des eaux de pluie hors de la zone des bassins. The invention is based on the placing on the ground of a set of gutters of great length, advantageously regularly spaced, so as to allow the installation of basins between the gutters, said gutters can ensure both the wall function basins, support of the cover of said basins, taxiway for operating personnel, support networks, collection and management of rainwater outside the basin area.
Ainsi l'invention a pour objet premier un dispositif de construction de parois de bassins couverts composé d'un chéneau profilé© de grande longueur, avantageusement posé au sol, qui peut faire office de paroi, avantageusement de paroi pour deux bassins juxtaposés, et dont le profil peut permettre la fixation sur toute la longueur du chéneau des films de fonds® et de couverture des bassins®. Thus, a first object of the invention is a device for constructing covered basement walls composed of a profiled trough © of great length, advantageously placed on the ground, which can act as a wall, advantageously a wall for two juxtaposed basins, and of which the profile can be used to fix the bottom and bottom films of the basins® for the entire length of the gutter.
L'invention a aussi pour objet un bassin couvert, caractérisé par la mise en oeuvre d'au moins un chéneau profilé© de grande longueur en tant que paroi latérale, avantageusement commune à deux bassins juxtaposés, et en tant que support de la couverture supérieure souple étanche à l'air et à l'eau de chaque bassin combinée à l'utilisation de films ou bâches souples en tant que fonds de bassins.  The invention also relates to a covered basin, characterized by the implementation of at least one profile gutter © of great length as a side wall, preferably common to two juxtaposed basins, and as a support for the upper cover flexible airtight and water-resistant of each basin combined with the use of films or flexible sheets as bas fonds.
Les dessins annexés illustrent l'invention.  The accompanying drawings illustrate the invention.
La figure 1 représente une vue du profil transversal d'un mode particulier de réalisation du chéneau.  Figure 1 shows a view of the transverse profile of a particular embodiment of the gutter.
La figure 2 représente une vue de dessus d'un ensemble de bassins réalisés à l'aide du chéneau  FIG. 2 represents a view from above of a set of basins made using the gutter
La figure 3 représente une vue en coupe transversale selon AA de la figure 2. FIG. 3 represents a cross-sectional view along AA of FIG. 2.
La figure 4 représente une vue de dessus d'un ensemble de bassins réalisés à l'aide du chéneau (variante de la figure 2). Figure 4 shows a top view of a set of basins made using the gutter (variant of Figure 2).
Le chéneau©, dispositif essentiel de l'invention peut prendre la forme habituelle connue de l'homme de l'art dans son usage traditionnel.  The gutter ©, essential device of the invention can take the usual form known to those skilled in the art in its traditional use.
Dans l'utilisation particulière propre à l'invention, ledit chéneau©, peut être de très grande longueur jusqu'à plusieurs centaines de mètres, avantageusement de 5 à 500 mètres, encore plus avantageusement de 10 à 300 mètres. Ledit chéneau© peut avoir un profil transversal sensiblement en forme de U avec un fond et des parois latérales, avantageusement tel que celui décrit par la figure 1. Le fond ©, éventuellement posé directement sur le sol peut être large de quelques dizaines de centimètres à plusieurs mètres, par exemple de 10 centimètres à 10 mètres, avantageusement de 30 centimètres à 3 mètres, encore plus avantageusement de 40 à 100 centimètres. Les parois latérales©, avantageusement verticales, peuvent être hautes de 5 à 100 centimètres, avantageusement de 10 à 30 centimètres peuvent présenter à leur extrémité supérieure un pli © qui peut courir sur toute la longueur du chéneau. In the particular use of the invention, said gutter © may be of very great length up to several hundred meters, advantageously of 5 to 500 meters, even more preferably from 10 to 300 meters. Said gutter © may have a substantially U-shaped transverse profile with a bottom and side walls, advantageously such as that described in FIG. 1. The bottom ©, which may be placed directly on the ground, may be a few tens of centimeters wide. several meters, for example from 10 centimeters to 10 meters, advantageously from 30 centimeters to 3 meters, even more advantageously from 40 to 100 centimeters. The side walls ©, preferably vertical, may be 5 to 100 centimeters high, preferably 10 to 30 centimeters may have at their upper end a fold © which can run the entire length of the gutter.
Le pli © longitudinal peut être destiné à i) assurer la fonction traditionnelle de rigidification de la paroi © pour en limiter la déformation ii) permettre l'accroche des films souples de fond et de couverture des bassins (voir ci après).  The longitudinal fold may be intended to i) provide the traditional function of stiffening the wall © to limit the deformation ii) allow the attachment of flexible films bottom and cover pools (see below).
Selon le mode particulier de réalisation, ce pli peut avoir une forme arrondie ou comporter des arêtes vives, être orienté vers l'extérieur ou vers l'intérieur du chéneau.  According to the particular embodiment, this fold may have a rounded shape or have sharp edges, be oriented outwardly or inwardly of the gutter.
Selon un mode particulier de réalisation non illustré, ledit pli © peut être dédoublé. Un premier pli peut être positionné au faîte de la paroi latérale © et peut en assurer la rigidification. Un second pli peut être positionné entre le faîte et la base de la paroi latérale © et peut assurer la fonction d'accroché des films souples (voir ci après).  According to a particular embodiment not illustrated, said fold © can be split. A first fold can be positioned at the ridge of the side wall © and can ensure the stiffening. A second fold can be positioned between the ridge and the base of the side wall © and can provide the function of hanging flexible films (see below).
Ledit chéneau nécessaire à la réalisation de l'invention peut être fabriqué par tout moyen technique approprié connu de l'homme de l'art tel qu'à titre d'exemples non limitatifs :  Said gutter necessary for carrying out the invention may be manufactured by any appropriate technical means known to those skilled in the art, as non-limiting examples:
i) profilage à froid d'une feuille de grande longueur (plusieurs dizaines ou centaines de mètres), avantageusement présentée en rouleau, d'un métal mince déformable, tel que l'aluminium, l'acier galvanisé ou inoxydable, ou tout autre métal pouvant être adapté en raison de ses caractéristiques techniques ou de son coût (technique de production des gouttières en métal par profileuse à froid).  i) cold profiling of a sheet of great length (several tens or hundreds of meters), advantageously presented in roll, of a deformable thin metal, such as aluminum, galvanized or stainless steel, or any other metal can be adapted because of its technical characteristics or its cost (production technique of metal gutters by cold forming machine).
ii) extrusion de profilé d'un matériau thermoplastique en sections de quelques mètres assemblées par collage, soudure, boulonnage ou rivetage pour obtenir la grande longueur requise (technique de production de tubes ou tuyaux PVC).  ii) profile extrusion of a thermoplastic material in sections of a few meters assembled by gluing, welding, bolting or riveting to obtain the required long length (PVC tube or pipe production technique).
iii) moulage de pièces de béton ou ciment aboutées pour obtenir la grande longueur requise (technique de production des caniveaux, ou des bordures de trottoirs).  iii) casting of cemented concrete or cement pieces to obtain the required long length (production technique of gutters, or curbs).
Selon le mode général de réalisation de l'invention les dits chéneaux peuvent être simplement posés sur un sol, éventuellement préalablement nivelé et si besoin compacté selon les techniques appropriées connues de l'homme de l'art. According to the general embodiment of the invention said gutters can be simply placed on a floor, possibly pre-leveled and if necessary compacted according to the appropriate techniques known to those skilled in the art.
Ces travaux de génie civil peuvent être réalisés de manière à : This civil engineering work can be done in such a way as to:
i) réaliser une surface support couvrant la totalité de la superficie d'un ensemble de n bassins solidaires  i) create a support surface covering the entire area of a set of n integral basins
ii) réaliser une planimétrie quasi parfaite pour que deux points quelconques de l'ensemble de la surface support définie en (i) aient un écart d'altitude maximum inférieur ou égal à 5 centimètres et idéalement inférieur ou égal à 2 centimètres, niveau de tolérance largement à la portée des entreprises spécialisées par exemple dans la réalisation de revêtements routiers. iii) obtenir une couche superficielle débarrassée de tout élément susceptible d'agresser les matériaux souples qui pourront y être déployés pour constituer les fonds des bassins®. La mise en place d'une couche de réglage de sable fin après épierrage est un exemple de solution pour atteindre cet objectif. ii) achieve a nearly perfect planimetry so that any two points of the entire support surface defined in (i) have a maximum altitude difference of less than or equal to 5 centimeters and ideally less than or equal to 2 centimeters, tolerance level widely available to specialized companies for example in the realization of road surfaces. iii) obtain a surface layer freed from any element likely to attack the soft materials that can be deployed to form the bottoms of the basins®. The establishment of a fine sand setting layer after stone removal is an example of a solution to achieve this objective.
Les chéneaux © peuvent être disposés sur la surface support ainsi réalisée, dans le respect des modalités suivantes :  The gutters © can be placed on the support surface thus produced, in the following ways:
i) sur un même ensemble, tous les chéneaux peuvent être idéalement réalisés à l'identique, quelque soit le mode particulier de réalisation, et peuvent avoir au minimum un profil transversal unique (même dessin, mêmes dimensions).  i) on the same set, all gutters can be ideally made identical, regardless of the particular embodiment, and can have at least a single transverse profile (same design, same dimensions).
ii) les chéneaux peuvent être posés strictement parallèlement.  (ii) the gutters may be laid strictly parallel.
iii) l'écart entre les chéneaux peut déterminer la largeur des bassins  iii) the gap between the gutters can determine the width of the basins
iiii) les chéneaux peuvent être de longueur sensiblement identique. Cette longueur peut déterminer celle des bassins qui de règle générale lui est légèrement inférieure.  iiii) the gutters may be of substantially identical length. This length can determine that of basins which generally is slightly lower.
iiiii) les chéneaux peuvent être fermement maintenus en place par tout moyen approprié au mode particulier de réalisation tel que par exemple 1) par le propre poids du chéneau réalisé en béton 2) par emplissage partiel par tout matériau approprié tel que sable, eau, gravier, béton ou autre, 3) par des crampons fixés dans le sol par tout moyen approprié à travers le fond CD ou tangentiellement aux parois©.  iiiii) the gutters can be firmly held in place by any means appropriate to the particular embodiment such as for example 1) by the own weight of the gutter made of concrete 2) by partial filling by any suitable material such as sand, water, gravel , concrete or other, 3) by crampons fixed in the ground by any suitable means through the bottom CD or tangentially to the walls ©.
Le fond des bassins peut être réalisé à l'aide d'un film souple ® étanche à l'eau dont la largeur est égale à l'écartement entre deux chéneaux augmenté du double de la hauteur de la paroi verticale © dudit chéneau. La longueur dudit film souple doit être égale à celle des chéneaux. Selon l'invention, ledit film souple ® peut être en tout matériau compatible avec la fonction prévue, tels que par exemple et sans limitation les membranes éthylène-propylène-diène monomère (EPDM), bâche en polychlorure de vinyle ou chlorure de polyvinyle (PVC), film polyéthylène (PE).  The bottom of the basins can be made using a waterproof flexible film ® whose width is equal to the spacing between two gutters plus double the height of the vertical wall © said gutter. The length of said flexible film must be equal to that of the gutters. According to the invention, said flexible film ® may be in any material compatible with the intended function, such as for example and without limitation the ethylene-propylene-diene monomer membranes (EPDM), polyvinyl chloride or polyvinyl chloride (PVC) ), polyethylene film (PE).
Les règles de l'art de la pose du film de fond de bassin peuvent nécessiter la pose préalable d'une couche de protection d'un matériau adapté comme à titre d'exemple un feutre géotextile.  The rules of the art of laying down the bottom film may require the prior installation of a protective layer of a suitable material such as for example a geotextile felt.
Selon le mode particulier de réalisation, les matériaux choisis, leurs caractéristiques techniques et les règles de l'art applicables, le fond de bassin peut être constitué de la superposition de plusieurs films souples.  According to the particular embodiment, the chosen materials, their technical characteristics and the applicable rules of art, the bottom of the basin may consist of the superposition of several flexible films.
La couverture du bassin ® peut être assurée par un film étanche à l'air et à l'eau et perméable à la lumière visible. Un film en polyéthylène d'usage courant en agriculture peut répondre à ces caractéristiques.  The pond cover ® can be provided by a film that is air and water tight and permeable to visible light. A polyethylene film commonly used in agriculture can meet these characteristics.
La largeur du film de couverture peut être sensiblement égale à celle du film de fond de bassin.  The width of the cover film may be substantially equal to that of the pelvis bottom film.
Chacun des deux films de fonds et de couverture du bassin peut être déroulé et disposé tendu et sans plis sur le sol et sur toute la superficie du bassin.  Each of the two fund and cover films of the pool can be unrolled and laid stretched and unfolded on the ground and over the entire basin area.
Les bords longitudinaux des deux films superposés peuvent être amenés ensemble contre le rebord supérieur d'un même coté du chéneau latéral pour y être fixé, par tout moyen adapté au mode particulier de réalisation sur toute la longueur du chéneau, de manière continue. Cette fixation peut être réalisée à titre d'exemple, par un boudin de caoutchouc comprimant les deux films à l'intérieur du pli®, ou par tout système ou dispositif adapté capable d'assurer : The longitudinal edges of the two superimposed films can be brought together against the upper edge of the same side of the side gutter to be fixed by any means adapted to the particular embodiment of the entire length of the gutter, continuously. This attachment can be made by way of example, by a rubber bead compressing the two films inside the ply®, or by any suitable system or device capable of providing:
i) le maintien en position du film de fond qui doit supporter la pression et le mouvement du liquide contenu dans le bassin.  i) maintaining in position the bottom film which must withstand the pressure and movement of the liquid contained in the basin.
ii) le maintien en position haute du film de couverture® qui doit supporter les effets du vent et de la mise en surpression de l'air contenu dans l'espace compris entre la surface du liquide contenu dans le bassin et le dit film de couverture.  ii) maintaining in the raised position the cover film which must withstand the effects of wind and overpressure of the air contained in the space between the surface of the liquid contained in the pool and said cover film .
iii) le maintien en position basse du film de couverture quand celui-ci est recouvert d'une couche d'eau, déposée volontairement dans le cadre de l'exploitation des bassins ou résultat involontaire mais prévisible d'un excès de pluie.  iii) keeping the cover film in a low position when it is covered with a layer of water, voluntarily deposited as part of the operation of the basins or an involuntary but predictable result of an excess of rain.
La réalisation des extrémités© des bassins demeure hors du champ de l'invention. Ces extrémités peuvent selon chaque mode particulier de réalisation, illustré par les figures 2 et 4, prendre des formes différentes tout en respectant deux règles communes :  The realization of the ends © of the basins remains outside the scope of the invention. These ends may according to each particular embodiment, illustrated by Figures 2 and 4, take different forms while respecting two common rules:
i) les parois des extrémités doivent assurer le maintien en position haute du film de fond de manière à assurer l'étanchéité du bassin et la nécessaire rétention du liquide contenu.  i) the walls of the ends must ensure the maintenance of the bottom film in the high position so as to ensure the tightness of the basin and the necessary retention of the liquid contained.
ii) la continuité de liaison entre les films souples de fonds et de couverture des doit être assurée de manière à maintenir le confinement du contenu liquide et gazeux du bassin, en intégrant, le cas échéant des dispositifs techniques rigides rendus nécessaires par tel ou tel mode particulier de réalisation des dits bassins. (ii) the continuity of the connection between the flexible film and the cover film must be ensured in such a way as to maintain the confinement of the liquid and gaseous contents of the pool, incorporating, where appropriate, rigid technical devices made necessary by one or other of these modes particular embodiment of said basins.
Pour chaque bassin construit selon l'invention : For each basin built according to the invention:
le chéneau peut représenter la seule structure rigide sur toute la longueur du bassin,  the gutter may be the only rigid structure along the entire length of the basin,
il peut n'y avoir aucune structure rigide transversale,  there may be no rigid transverse structure,
le maintien en position haute de la couverture peut être assuré par la mise en surpression de l'air ou du ou des gaz contenus dans le dit bassin. Cette surpression peut être obtenue par tout dispositif adapté connu de l'homme de l'art comme un ventilateur, un compresseur ou une soufflante.  the maintenance in the high position of the cover can be ensured by placing overpressurized air or gas or gases contained in said basin. This overpressure can be obtained by any suitable device known to those skilled in the art such as a fan, a compressor or a fan.
La couverture du bassin peut être mobile et à altitude variable, et peut, selon les besoins de l'exploitation être maintenue en position normale haute ou en position basse au contact du liquide contenu dans le bassin par simple arrêt des dispositifs surpresseurs.  The basin cover may be mobile and at variable altitude, and may, depending on the needs of the operation be kept in the normal up position or in the low position in contact with the liquid contained in the basin simply by stopping the blower devices.
Cette disposition permet une mise en sécurité des bassins i) par limitation à l'extrême de la prise au vent des bassins ii) par submersion de la couverture : la hauteur du liquide dans le bassin est obligatoirement inférieure à celle des parois © des chéneaux. Il est possible de répandre rapidement par-dessus le film de couverture (étanche à l'air et à l'eau) la quantité d'eau suffisante pour couvrir de quelques centimètres la totalité de la superficie du bassin. Cette opération permet de présenter l'ensemble des bassins comme un espace liquide et ainsi de faire face à des vents très violents susceptibles d'endommager ou détruire les installations et en particulier les couvertures des bassins si elles étaient maintenues en position haute. La remise en surpression de l'intérieur du bassin peut permettre la reprise du fonctionnement normal de l'installation : le gonflement de la couverture et son élévation de proche en proche chasse la couche d'eau supérieure qui coule vers les chéneaux latéraux et peut être évacuée selon le mode normal de fonctionnement. This arrangement makes it possible to make the basins safe i) by limiting the wind catchment of the basins to a minimum ii) by submerging the cover: the height of the liquid in the basin is necessarily lower than that of the walls © of the gutters. It is possible to quickly spread over the cover film (airtight and watertight) the amount of water sufficient to cover a few centimeters the entire area of the basin. This operation makes it possible to present all the basins as a liquid space and thus to face very violent winds likely to damage or destroy the installations and in particular the covers of the basins if they were kept in the high position. Overpressurisation of the interior of the basin may allow the resumption of normal operation of the installation: the swelling of the cover and its elevation from close to hunting flush the upper layer of water flowing to the side gutters and can be evacuated according to the normal mode of operation.
Selon un mode particulier de mise en œuvre de l'invention, pour renforcer la résistance au vent de l'ensemble, on peut disposer échelonnés sur toute la longueur des chéneaux des raidisseurs®. Le raidisseur a deux fonctions : i) maintenir strictement l'écartement des parois© du chéneau à la largeur du fond®, ii) comprimer le rebord (D du chéneau pour en contrôler l'ouverture et sécuriser la fixation des films (D et ®.  According to a particular embodiment of the invention, to increase the wind resistance of the assembly, it is possible to have staggered over the entire length of the gutters stiffeners. The stiffener has two functions: i) to strictly maintain the spacing of the walls © of the gutter to the width of the bottom, ii) to compress the rim (D of the gutter to control the opening and secure the fixing of the films (D and ® .
Selon un mode général de réalisation de l'invention, en rapport avec la fonction de collecte des eaux de pluie et la grande longueur des bassins, l'évacuation des eaux par les seules extrémités des chéneaux peut s'avérer insuffisante en fonction de la section transversale du chéneau.  According to a general embodiment of the invention, in relation to the rainwater collection function and the long length of the basins, the evacuation of water by the only ends of the gutters may be insufficient depending on the section. transverse of the gutter.
Pour y remédier il peut être utile de disposer des évacuations intermédiaires ©, avantageusement régulièrement espacées sur la longueur des chéneaux en relation avec le régime des pluies de la zone géographique particulière de l'installation (par exemple tous les cinquante mètres).  To remedy this, it may be useful to have intermediate evacuations ©, advantageously regularly spaced along the length of the gutters in relation to the rainfall regime of the particular geographical area of the installation (for example every fifty meters).
Lesdites évacuations peuvent être reliées à une conduite souterraine®, avantageusement disposée perpendiculairement, aux chéneaux. Chaque conduite souterraine peut être réalisée en matériaux et aux dimensions adaptés aux règles de l'art, et peut être installée préalablement à la pose des chéneaux © et des films de fonds de bassin (D et peut aboutir de part et d'autre de la zone de bassins qu'elle dessert à un fossé ® ou conduite principale qui a pour fonction d'amener les eaux collectées hors de la zone des bassins. Said evacuations may be connected to an underground pipe, advantageously disposed perpendicularly, to the gutters. Each underground pipe can be made of materials and dimensions adapted to the rules of art, and can be installed prior to the installation of the gutters © and the films of basin ( D and can end on both sides of the basin area it serves to a ditch ® or main pipe whose function is to bring collected water out of the basin area.
Les avantages de l'invention pour la construction des bassins de grandes dimensions résident i) dans l'économie de moyens : un seul chéneau sert de paroi à deux bassins contigus, de structure de support à la couverture des dits bassins, et de moyen de collecte et d'évacuation des eaux de pluie, ii) dans la rapidité d'installation des bassins iii) dans l'optimisation de l'occupation du terrain par réduction drastique de l'espace entre les bassins iiii) dans une prise au vent réduite par limitation de la hauteur de la couverture à moins du quart de la largeur du bassin.  The advantages of the invention for the construction of large basins reside i) in the economy of means: a single guteau serves as wall to two contiguous basins, support structure to the cover of said basins, and means of collection and disposal of rainwater, ii) rapidity of installation of basins iii) optimization of land use by drastically reducing the space between basins iiii) reduced wind intake limiting the height of the cover to less than one quarter of the width of the pool.
L'invention est particulièrement destinée à l'équipement de fermes de cultures de micro algues et d'organismes photosynthétiques, de grande taille, en particulier telles qu'elles peuvent résulter de la mise en œuvre de l'invention objet du brevet français n° 2 918 387.  The invention is particularly intended for the equipment of large scale microalgae and photosynthetic culture farms, in particular such that they may result from the implementation of the invention which is the subject of French patent no. 2,918,387.
L'invention a également pour objet un dispositif de construction de parois de bassins couverts selon l'invention, composé d'un chéneau profilé de grande longueur posé au sol, qui fait office de paroi pour deux bassins juxtaposés et dont le profil permet la fixation sur toute la longueur du chéneau des films de fonds et de couverture des bassins.  The subject of the invention is also a device for constructing covered pool walls according to the invention, composed of a long profiled gutter placed on the ground, which acts as a wall for two juxtaposed basins and whose profile allows the fixing along the entire length of the basement films and basins.
L'invention a également pour objet l'utilisation de bassins selon l'invention dans des fermes d'aquaculture ainsi que lesdites fermes d'aquaculture comprenant au moins un bassin selon l'invention.  The invention also relates to the use of tanks according to the invention in aquaculture farms and said aquaculture farms comprising at least one basin according to the invention.

Claims

REVENDICATION CLAIM
1. ) Dispositif de construction de parois de bassins couverts composé d'un chéneau profilé© de grande longueur avantageusement posé au sol, qui fait office de paroi, avantageusement de paroi pour deux bassins juxtaposés, et dont le profil permet la fixation sur toute la longueur du chéneau des films de fonds® et de couverture des bassins®. 1.) Covered basement wall construction device composed of a profiled gutter © of great length advantageously placed on the ground, which acts as a wall, preferably wall for two juxtaposed basins, and whose profile allows the attachment over the entire gutter length of the fonds® and pond cover® films.
2. ) Dispositif selon la revendication 1 , caractérisée par le positionnement de raidisseurs© transversaux régulièrement espacés sur toute la longueur dudit chéneau profilé®.  2.) Device according to claim 1, characterized by the positioning of transverse stiffeners regularly spaced along the entire length of said profile gutter.
3. ) Bassin couvert, caractérisé par la mise en œuvre d'au moins un dispositif selon l'une quelconque des revendications 1 ou 2, composé d'un chéneau de grande longueur en tant que paroi latérale, avantageusement commune à deux bassins juxtaposés, et en tant que support de la couverture supérieure souple étanche à l'air et à l'eau de chaque bassin combinée à l'utilisation de films ou bâches souples en tant que fonds de bassins.  3.) Covered basin, characterized by the implementation of at least one device according to any one of claims 1 or 2, composed of a guteau of great length as a side wall, preferably common to two basins juxtaposed, and as a support for the flexible, airtight top cover and water cover of each pool combined with the use of films or soft covers as basins.
4. ) Bassin selon la revendication 3, caractérisé en ce qu'il comprend une couverture de bassin sans structure rigide portante transversale et à altitude variable directement supportée par les parois du chéneau.  4.) A basin according to claim 3, characterized in that it comprises a basin cover without transverse load bearing rigid structure and variable altitude directly supported by the walls of the gutter.
5. ) Bassin selon l'une quelconque des revendications 3 ou 4, caractérisé en ce qu'il comprend un dispositif de sécurisation des installations par submersion de la couverture souple et étanche des bassins.  5.) Basin according to any one of claims 3 or 4, characterized in that it comprises a device for securing facilities by submersion of the flexible cover and waterproof basins.
6. ) Bassin l'une quelconque des revendications 3 à 5, caractérisée en ce que des dispositifs d'évacuations" destinés à l'écoulement des eaux de pluies sont positionnés échelonnés sur la longueur d'un chéneau. 6.) Basin any one of claims 3 to 5, characterized in that evacuation devices " for the flow of rainwater are positioned staggered along the length of a gutter.
7. ) Utilisation d'un dispositif selon l'une quelconque des revendications 1 ou 2 dans la construction de bassins.  7.) Use of a device according to any one of claims 1 or 2 in the construction of basins.
8. ) Utilisation selon la revendication 7 dans une ferme d'aquaculture, particulièrement une ferme destinée à la culture d'organismes photosynthétiques, très particulièrement une ferme d'aquaculture de microalgues.  8.) Use according to claim 7 in an aquaculture farm, particularly a farm for the cultivation of photosynthetic organisms, particularly a microalgae aquaculture farm.
EP11723192A 2010-04-29 2011-04-28 Tanks for culturing photosynthetic organisms Withdrawn EP2563900A2 (en)

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FR1001832A FR2959513B1 (en) 2010-04-29 2010-04-29 METHOD AND DEVICE FOR CONSTRUCTING CULTURE BASINS OF PHOTOSYNTHETIC ORGANISMS
PCT/FR2011/000259 WO2011135208A2 (en) 2010-04-29 2011-04-28 Tanks for culturing photosynthetic organisms

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JP2016512687A (en) 2013-03-15 2016-05-09 ヘリアエ デベロップメント、 エルエルシー Large-scale mixed nutrient production system
FR3018303A1 (en) * 2014-03-04 2015-09-11 Jouin Solutions Plastiques BASIN PARTICULARLY FOR THE CULTURE OF LIVING ORGANISMS

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BR112012027609A2 (en) 2015-11-24

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