EP2482982B1 - Flachkörper nach art einer chip-karte zur biochemischen analyse - Google Patents

Flachkörper nach art einer chip-karte zur biochemischen analyse Download PDF

Info

Publication number
EP2482982B1
EP2482982B1 EP10760321.9A EP10760321A EP2482982B1 EP 2482982 B1 EP2482982 B1 EP 2482982B1 EP 10760321 A EP10760321 A EP 10760321A EP 2482982 B1 EP2482982 B1 EP 2482982B1
Authority
EP
European Patent Office
Prior art keywords
flat body
sensor chip
microfluidic device
cup
flat
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Not-in-force
Application number
EP10760321.9A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP2482982A1 (de
Inventor
Walter Gumbrecht
Peter Paulicka
Jörn UEBERFELD
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Vetmedica GmbH
Original Assignee
Boehringer Ingelheim Vetmedica GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Vetmedica GmbH filed Critical Boehringer Ingelheim Vetmedica GmbH
Publication of EP2482982A1 publication Critical patent/EP2482982A1/de
Application granted granted Critical
Publication of EP2482982B1 publication Critical patent/EP2482982B1/de
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0663—Whole sensors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0672—Integrated piercing tool
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0883—Serpentine channels
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082—Test tubes per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Definitions

  • the present invention relates to a flat body in the manner of a chip card for the biochemical analysis of substances according to the preamble of claim 1.
  • the flat body has at least two microfluidic devices and at least one sensor chip.
  • the at least one sensor chip is integrated in the flat body and is in direct contact with at least one first microfluidic device.
  • a flat body for biochemical analysis of substances such as DNA and proteins known.
  • This flat body has the shape of a chip card, which is designed analogously to a credit card.
  • the flat body includes a semiconductor chip having a sensor array and integrated circuits, the semiconductor chip being potted in a flat plastic material and being electrically connected to electrical contacts for reading the chip through an external readout unit.
  • On a front side of the flat body are formed as recesses in the plastic microfluidic device such as reaction chambers and channels. The front side is covered with a film and the microfluidic devices are so fluid-tight with respect to the environment, that is tightly sealed against liquids and / or gases.
  • the film of the chip card is pierced via a pointed needle analogous to a syringe tip, and the liquid is injected into a microfluidic device of the chip card.
  • the liquid comes into contact with sensors of the sensor array on the chip and constituents of the liquid can be detected directly or indirectly. Detection may be optical or electrochemical. Substances which are necessary for chemical reactions to detect the constituents of the liquid can already be located on or in the chip card or can likewise be injected into this via a pointed needle.
  • the absorption capacity of microfluidic devices on a chip card for receiving liquid is usually very low and is often limited to only a few milliliters, or to microliters or in extreme cases only to nanoliters.
  • Another problem with supplying liquid to or into the chip card via pointed needles may be the introduction of contaminants.
  • the slightest chemical or biochemical impurities can lead to errors in the quantitative and / or qualitative detection.
  • any additional device e.g. a needle with which the liquid to be examined is brought into contact increases the probability of contamination.
  • An increased effort, which is cost and time consuming, must be provided to ensure the detection quality, e.g. by thorough cleaning of all devices.
  • the device comprises a reaction chamber and a flat body.
  • the sample is amplified within the reaction chamber using reagents. Subsequently, the amplified sample is transferred to the flat body, wherein the flat body has an optical detection device for detecting the nucleic acids.
  • the WO 2009/115608 A2 relates to a cartridge system for sample preparation and sample analysis, wherein the cartridge system comprises a flat body and a sample container.
  • the sample container can be inserted linearly into the flat body so that a fluid transfer of the sample from the sample container to the flat body takes place.
  • Detection devices for analyzing the sample are provided in the flat body.
  • the object of the present invention is therefore to provide a flat body in the form of a chip card for biochemical analysis, in which it is possible in a simple and cost-effective manner, fluids such.
  • Liquids to introduce directly from a vessel in microfluidic devices of the flat body.
  • it is an object to introduce fluids into the microfluidic devices of the flat body, wherein the fluids are brought into contact with or flow through as few autarkic individual parts as possible.
  • the flat body according to the invention in the manner of a chip card for the biochemical analysis of substances comprises at least two microfluidic devices and at least one sensor chip.
  • the at least one sensor chip is integrated in the flat body and is in direct contact with at least one first microfluidic device.
  • the flat body integrally comprises a second microfluidic device in the manner of a pipette. Integral means that the second microfluidic device and the remaining flat body are made of at least one material together and form a coherent body, without the second microfluidic device is attached to the flat body, clamped or otherwise repeatedly attached separable and fastened.
  • the advantage of a flat body with integrated pipette lies in the possibility of large quantities of liquid between a vessel, as e.g. represents an e-cup, and the flat body easy and fast exchange. Since the flat body and the pipette integrated therein can be made of one material together, both have the same chemical and biochemical purity levels. An entrainment of impurities by additional parts in the flat body is thus prevented.
  • the possible production in one step reduces costs and effort and leads to a higher stability than plugging solutions of e.g. Syringe cannula needles made of metal.
  • the flat body may comprise a first clamping device, which is designed to attach an e-cup directly mechanically to the flat body.
  • E-cups are used as reaction vessels and are available eg from Eppendorf® and then known by the short form "Eppi". By default, the vessels have different sizes and can accommodate different volumes of solution, eg from 0.2 ml to 2 ml. They are characterized by good chemical resistance and are dimensionally stable up to more than 100 ° C.
  • the clamping device would have a diameter substantially equal to the inner diameter of an e-cup to be fastened at its opening. A mechanical attachment of the e-cup directly to the flat body by the terminals represents a particularly simple and stable way to attach the e-cup to the flat body.
  • the flat body may comprise a second clamping device, which is designed to fasten a lid of an e-cup directly mechanically to the flat body. This increases the stability of the attachment of an E-cup to the flat body and leads to an improvement of the handling, since the lid does not disturb movably relative to the flat body during filling or the removal of liquid from the E-Cup.
  • the second microfluidic device may be elongated and comprise at one end a tip with a fluidic opening. It can be designed so that when mounting an E-cup on the first and / or second clamping device, the tip of the second microfluidic device is arranged with the fluidic opening in the region of a lower end of the E-cup. As a result, an almost complete removal of liquid from the e-cup is made possible with the aid of the second microfluidic device.
  • the flat body may consist of a plastic material, in particular an injection-molded plastic. Injection molding plastic is easy to work with and enables cost-effective production of the flat body.
  • the microfluidic devices may be formed on a front side of the flat body and covered with a foil, in particular a self-adhesive foil of plastic material. This allows a simple and cost-effective production of the flat body with microfluidic devices.
  • the at least two microfluidic devices may include channels and / or chambers, which are formed as depressions in a flat plane of the front side of the flat body. Furthermore, the at least two microfluidic devices may comprise valves formed in the flat body. The at least two microfluidic devices may also comprise a recess, which is formed as a recess in a flat plane of the rear side of the flat body and in which the sensor chip embedded is, in particular with electrical contacts of the sensor chip in a plane with the flat plane of the back of the flat body and with a sensor array of the sensor chip in direct contact with at least one chamber on the front side of the flat body.
  • the at least two microfluidic devices are thereby suitable for facilitating good handling of liquids and for transporting liquids from an e-cup to sensors on the chip.
  • chemical reactions of liquids or substances in the liquids can take place eg in chambers with solid phase reagents.
  • the flat body may have a thickness in the range of one millimeter, a length in the range of 85 millimeters and a width in the range of 54 millimeters.
  • At least one microfluidic device may be configured to include dry reagents, especially in channels and / or reaction spaces having a cross-section in the range of one or more square millimeters.
  • the second microfluidic device may have a length in the range of 45 millimeters.
  • the second microfluidic device may be in fluidic contact with sensors of the sensor chip via the first microfluidic device.
  • a cross section through the second microfluidic device, perpendicular to the front side of the flat body, may have a substantially rectangular outer periphery with an open recess towards the front side of the flat body.
  • the sensor chip may comprise an array of electrochemical sensors. As a result, with the flat body electrochemical measurements are possible, which are easier, cheaper and better to perform in the smallest space than optical measurements.
  • the sensor chip may further comprise an integrated circuit for processing electrical signals from the sensors.
  • the sensor chip can also comprise electrical contacts for the electrical readout of the sensor chip, in particular for the electrical readout of the sensor chip with the aid of an external data processing unit.
  • the flat body can have at least one opening on its front and / or rear side, which is in fluidic contact with the at least one first microfluidic device, and / or which is designed to connect an external pump.
  • small amounts of substances used for the detection in particular in liquid form, can be supplied to the flat body via this opening or openings.
  • a negative pressure in the microfluidic devices can also be achieved via the at least one opening, e.g. be generated by means of a pump and serve to suck liquid from an e-cup in the flat body or its microfluidic devices into it.
  • the second microfluidic device can take up liquid from the E-cup in a first step and deliver liquid into the E-cup in a second step, wherein in particular the first and the second step are repeated at intervals.
  • This allows a kind of rinsing of the microfluidic devices with liquid from the e-cup.
  • reactions which require a large amount of solution with large volume, not perform in the microfluidic devices, but in a docked E-Cup.
  • a combination of reactions in the E-cup and the microfluidic devices in different order is also possible.
  • a liquid to be examined for example, blood, urine, fresh or waste water
  • the flat body according to the invention and the method for its use are particularly well suited but are not limited to being used at low concentrations of substance to be detected and large volumes of solution of the liquid required for detection. If the concentration of the substance to be detected is so low that a volume of liquid necessary for the detection exceeds the capacity of the microfluidic devices formed in or on the flat body, reactions can be carried out in a docked E cup and the ready-reacted liquids can be carried out via the second microfluidic Device are supplied to the sensors of the sensor chip in the flat body.
  • the sensors of the sensor chip can detect, for example, DNA, RNA, peptides or antibodies.
  • Substances which are involved in the detection and in the preparation can be stored, for example, in chambers or channels of the flat body, in particular as dry reagents.
  • the chemical reaction can be liquid an e-cup are sucked into the microfluidic devices and mixed with the stored substances, eg for dissolving dry reagents, and then released again to the e-cup. In the e-cup can then react a larger volume of fluid than in the microfluidic devices.
  • a portion of the liquid in the e-cup may be drawn over the first into the second microfluidic device, eg by applied vacuum to openings of the first microfluidic device, and at the sensors detection of reaction products or directly from in the liquid contained substances.
  • Fig. 1 is merely for explanatory purposes a plan view of a front side 7 of the flat body without cover and a section through a E cup 5 shown.
  • the flat body 1 is in the form of a chip card or in the form of a credit card. Values for the size ratios of such a chip card are, for example, height H x width B x thickness D equal to 5.5 cm x 8.5 cm x 0.1 cm.
  • On the front side 7 microfluidic devices 4, 7 are formed as depressions in the flat body 1.
  • the flat body 1 consists for example of a plastic material, in particular an injection-molded plastic.
  • Microfluidic devices 4 are, for example, channels 9 and chambers 10, which may have a width in the range of 1 mm to 5 mm and a depth of about 100 ⁇ m.
  • chambers may have a length of 1mm to 10mm and channels may have a length in the range of 1cm to 100cm.
  • reagents can be stored, for example, in dried form.
  • a sensor chip 2 is fixed, for example by gluing.
  • the sensor chip 2 with a sensor array on one side and electrical contacts for reading the sensor chip 2 on the other side of the sensor chip 2 is arranged in the recess such that the side of the sensor chip 2 with the sensor -Array forms the bottom of a microfluidic chamber 10 ', which serves as a reaction and / or detection chamber.
  • the side of the sensor chip 2 with the electrical contacts forms with the rear side 8 of the flat body 1 a plane.
  • Sensors of the sensor array can detect optically or electrochemically substances or reaction products in a liquid which is located in the microfluidic chamber 10 '. Electrical signals from the sensors can be delivered via the electrical contacts of the sensor chip 2 to external measuring and data processing devices or processed by integrated circuits on the sensor chip 2 and displayed directly or transmitted via the electrical contacts.
  • the microfluidic devices 3, 9, 10, 10 ' are supplied.
  • a control of the supply can be effected via valves 11, which are formed in the flat body 1. It is also fluids such as air via the inlet and outlet openings 12 are supplied to the flat body or removed, with a positive or negative pressure in the microfluidic devices 3, 9, 10, 10 'is generated.
  • the flat body 1 comprises a second microfluidic device 4 which has the shape and function of a flattened pipette.
  • the second microfluidic device 4 is in one piece with the flat body made of e.g. Made of plastic.
  • the length L may be in the range of 2.5 cm, depending on the size of an e-cup 5 to be used. The length should be close to the depth of the e-cups 5, i. the distance from the opening 15 to the bottom 14 of the e-cup 5 amount. As a result, an almost complete removal of liquid from an e-cup 5 with the aid of the second microfluidic device 4 is made possible.
  • the thickness of the second microfluidic device 4 is equal to the thickness of the flat body, e.g. 1 mm.
  • a channel 9 ' is formed as a depression which corresponds approximately to the size of channels 9 of the first microfluidic device 3 in the remaining flat body 1. So its width is in the range of 1mm and its depth in the range of 100 ⁇ m.
  • the channel 9 ' is fluidically connected via channels 9 and / or chambers 10 with sensors of the sensor chip 2.
  • the width of the second microfluidic device 4 is e.g. 2mm.
  • an e-cup 5 are attached to the flat body 1 by clamping.
  • a section through an e-cup 5 is shown.
  • reaction vessels in the form of "Eppis" can be used which absorb, for example, a volume of liquid in the range of 1ml to 100ml.
  • liquid can be contained in the e-cup 5 to be examined liquid such as blood, urine, process water or drinking water.
  • This liquid can be prepared in the e-cup 5 for examination.
  • cells can be digested, DNA multiplied, markers coupled and / or a fish out or concentrated by means of certain molecules in the e-cup 5 done.
  • the liquid to be examined can be introduced untreated via the second microfluidic device 4 into the flat body 1.
  • liquid may be contained in the e-cup 5 instead of the liquid to be examined in a study substances involved.
  • the second microfluidic device 4 is fluidically connected to the first microfluidic device 3 and is introduced into an e-cup 5 such that liquid from the e-cup 5 via the second microfluidic device 4 via capillary forces or a negative pressure in the first microfluidic device 3 into the first microfluidic device 3 and the sensor array of the sensor chip 2 passes. Via an overpressure in the first microfluidic device 3, fluid from the first microfluidic device 3 can be introduced into the e-cup 5 via the second microfluidic device 4.
  • Chemical reactions that require a lot of solution volume and therefore can not be performed in a microfluidic device 3 take place in the e-cup "outsourced”. Subsequently, the reaction product in the flat body 1 can be further processed or detected directly via the sensors.
  • the clamping device 6a is formed as a broadening of the second microfluidic device 4. This makes possible a simple and cost-effective production of the clamping device 6a in one step together with the flat body 1 including the second microfluidic device 4 as an integral body made of injection-molded plastic.
  • the Microfluidic devices 3, 4 are sealed by means of a foil.
  • a self-adhesive and / or glued foil completely cover the front side 7 of the flat body 1, including the first and second microfluidic devices 3, 4.
  • a thermally welded film can be applied partially or completely on the flat body 1.
  • the openings 12 can be pierced if necessary by needles.
  • An opening at the tip 13 of the second microfluidic device 4 may also be made by tearing, cutting or piercing as needed, or alternatively, when a foil is applied to the flat body 1, the opening at the tip 13 may be formed.
  • the clamping device 6a has substantially a width corresponding to the inner diameter of the opening 15 of the E-cup or is slightly, for example about 1mm, larger.
  • the simplest form of the clamping device is rectangular, in particular with rounded corners.
  • Friction leads to a mechanical clamping of the e-cup 5 to the flat body 1, especially to the clamping device 6a of the Flat body 1.
  • a simple pushing on the E-cup 5 on the clamping device 6a is also given if the clamping device 6a has the outline of a section through a barrel, with convex bulges on the two opposite edges.
  • Fig. 1 only a rectangular shape of the clamping device 6a shown.
  • the thickness of the clamping device is equal to or substantially equal to the thickness of the remaining flat body. 1
  • FIG. 2 an embodiment of the flat body 1 with a clamping device 6a and a clamping device 6b is shown.
  • the clamping device 6a is analogous to the previously described clamping device 6a.
  • a clamping device 6b for clamping a lid of an e-cup 5 is formed in the flat body 1.
  • the clamping device 6b is made of two recesses in an edge 17 of the flat body 1 adjacent to the second microfluidic device 4 constructed.
  • the recesses with their dimensions have the inverse shape and dimensions of the lower lid part, which in the closed E-cup 5 in the direction of e-cup 5 has.
  • the clamping device 6b leads to an improved mechanical connection of an E-cup 5 with the flat body 1 and increased stability of an arrangement E-cup 5 and flat body 1.
  • a simple handling of flat body 1 in conjunction with an E-cup 5 is allowed.
  • An e-cup 5 can be used in conjunction with the flat body 1 as Sample vessel for supplying the investigated or involved in the reaction of liquids serve as an external reaction vessel or serve as a waste container for liquids to be disposed of.
  • the total length of the E-Cup 5 is 30mm and the length in the interior of the E-Cup 5 29mm.
  • the outer diameter of the E-Cup 5 is 7.6mm. Decisive for the dimensions of the clamping device 6a, however, the outer diameter of 10 mm and the inner diameter of 6.5 mm of the circular upper edge of the E-cup 5, which has the shape of a brim.
  • the clamping device 6a thus also has a width in the range of 6.5 mm or slightly larger, for example, 6.6 mm in this embodiment. As a result, a mechanical attachment is achieved by clamping when pushing the e-cup 5.
  • the distance between the transition of the clamping device 6a to the remaining flat body 1 in relation to the tip 13 of the clamping device 6a is 29mm or slightly less at a length of the interior of the e-cup. This ensures that when pushed the E-cup until it stops at the transition of the clamping device 6a to the rest of the flat body 1, the tip 13 in the region of the bottom 14 of E-cup 5 is arranged. Thus, all the liquid in an e-cup 5 can be handled by the second microfluidic device 4.
  • the length of the distance of the transition of the clamping device 6a to the remaining flat body 1 in relation to the tip 13 of the clamping device 6a may be formed longer than 29mm. In the event that the entire liquid volume of the E-Cup 5 does not have to be used or handled, the length can also be shorter than 29 mm.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
EP10760321.9A 2009-09-28 2010-09-27 Flachkörper nach art einer chip-karte zur biochemischen analyse Not-in-force EP2482982B1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102009043226A DE102009043226B4 (de) 2009-09-28 2009-09-28 Flachkörper nach Art einer Chip-Karte zur biochemischen Analyse und Verfahren zu dessen Verwendung
PCT/EP2010/064258 WO2011036289A1 (de) 2009-09-28 2010-09-27 Flachkörper nach art einer chip-karte zur biochemischen analyse und verfahren zu dessen verwendung

Publications (2)

Publication Number Publication Date
EP2482982A1 EP2482982A1 (de) 2012-08-08
EP2482982B1 true EP2482982B1 (de) 2017-08-16

Family

ID=43302368

Family Applications (1)

Application Number Title Priority Date Filing Date
EP10760321.9A Not-in-force EP2482982B1 (de) 2009-09-28 2010-09-27 Flachkörper nach art einer chip-karte zur biochemischen analyse

Country Status (7)

Country Link
US (1) US9415390B2 (ja)
EP (1) EP2482982B1 (ja)
JP (1) JP5430766B2 (ja)
CN (1) CN102548659B (ja)
BR (1) BR112012006831B1 (ja)
DE (1) DE102009043226B4 (ja)
WO (1) WO2011036289A1 (ja)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102009043226B4 (de) 2009-09-28 2012-09-27 Siemens Aktiengesellschaft Flachkörper nach Art einer Chip-Karte zur biochemischen Analyse und Verfahren zu dessen Verwendung
EP2514528A1 (en) * 2011-04-19 2012-10-24 Cellix Limited Device and method for assessing the status of cells in a biological fluid
EP2785460B1 (en) * 2011-11-29 2021-01-27 Caliper Life Sciences, Inc. Systems and methods for sampling of amplification products
US9689029B2 (en) 2011-12-02 2017-06-27 Caliper Life Sciences, Inc. Systems and methods for sampling of amplification products
US10241120B2 (en) * 2011-12-06 2019-03-26 Universite Libre De Bruxelles Method and device for assaying an antigen present on erythrocytes or an antibody binding to an antigen present on erythrocytes
CN104178413B (zh) * 2014-07-04 2016-05-25 宁波美晶医疗技术有限公司 一种稀有细胞分离仪的塑封盒包装结构
US10086368B2 (en) 2015-09-07 2018-10-02 EXIAS Medical GmbH Movable measurement cell
CN110624615B (zh) * 2019-09-19 2024-08-06 深圳富士凯烨医疗电子科技有限公司 一种微流控芯片

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747666A (en) * 1997-03-26 1998-05-05 Willis; John P. Point-of-care analyzer module
US5804437A (en) * 1997-08-19 1998-09-08 Biomerieux Vitek, Inc. Locking structure for securing a fluid transfer tube
DE19846466A1 (de) * 1998-10-08 2000-04-27 Ghs Gesundheits Service Ag Analyseverfahren zur simultanen Bestimmung von Parametern aus unterschiedlichen Medien
DE19964337B4 (de) * 1999-10-01 2004-09-16 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto Mikrofluidischer Mikrochip mit abbiegbarem Ansaugrohr
CN1117284C (zh) 1999-10-27 2003-08-06 陆祖宏 微流体生物芯片检测分析板及其应用方法
US20020009392A1 (en) 2000-03-28 2002-01-24 Wolk Jeffrey A. Methods of reducing fluid carryover in microfluidic devices
DE10111458B4 (de) 2001-03-09 2008-09-11 Siemens Ag Analyseeinrichtung
US6913933B2 (en) * 2001-12-03 2005-07-05 Ortho-Clinical Diagnostics, Inc. Fluid dispensing algorithm for a variable speed pump driven metering system
US7524464B2 (en) * 2003-09-26 2009-04-28 Ahn Chong H Smart disposable plastic lab-on-a-chip for point-of-care testing
DE102005049976A1 (de) 2004-10-15 2006-04-20 Siemens Ag Anordnung zur integrierten und automatisierten DNA- oder Protein-Analyse in einer einmal verwendbaren Cartridge, Herstellungsverfahren für eine solche Cartridge und Betriebsverfahren der DNA- oder Protein-Analyse unter Verwendung einer solchen Cartridge
US20060165558A1 (en) 2004-12-21 2006-07-27 Thomas Witty Cartridge for diagnostic assays
US8206650B2 (en) * 2005-04-12 2012-06-26 Chromedx Inc. Joint-diagnostic spectroscopic and biosensor meter
WO2007021809A2 (en) * 2005-08-11 2007-02-22 Eksigent Technologies, Llc Microfluidic systems, devices and methods for reducing background autofluorescence and the effects thereof
EP1963819A2 (en) * 2005-12-22 2008-09-03 Honeywell International, Inc. Portable sample analyzer system
WO2008002483A2 (en) * 2006-06-23 2008-01-03 Mcneely Michael R Reagent preparation and valving design for liquid testing
US8163535B2 (en) 2006-06-26 2012-04-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
CN101479609B (zh) 2006-06-30 2012-09-05 松下电器产业株式会社 分析用面板以及使用该面板的分析装置
JP2008175608A (ja) 2007-01-17 2008-07-31 Yokogawa Electric Corp 化学反応用カートリッジ及びその使用方法
US20090186344A1 (en) * 2008-01-23 2009-07-23 Caliper Life Sciences, Inc. Devices and methods for detecting and quantitating nucleic acids using size separation of amplicons
GB0805296D0 (en) 2008-03-20 2008-04-30 Iti Scotland Ltd Uses of reagents in sample collection and cartridge systems
DE102009043226B4 (de) 2009-09-28 2012-09-27 Siemens Aktiengesellschaft Flachkörper nach Art einer Chip-Karte zur biochemischen Analyse und Verfahren zu dessen Verwendung

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
None *

Also Published As

Publication number Publication date
JP2013506123A (ja) 2013-02-21
US9415390B2 (en) 2016-08-16
CN102548659A (zh) 2012-07-04
BR112012006831A2 (pt) 2016-06-07
BR112012006831A8 (pt) 2017-12-05
BR112012006831B1 (pt) 2020-02-04
JP5430766B2 (ja) 2014-03-05
US20120184043A1 (en) 2012-07-19
WO2011036289A1 (de) 2011-03-31
DE102009043226B4 (de) 2012-09-27
DE102009043226A1 (de) 2011-03-31
EP2482982A1 (de) 2012-08-08
CN102548659B (zh) 2016-12-07

Similar Documents

Publication Publication Date Title
EP2482982B1 (de) Flachkörper nach art einer chip-karte zur biochemischen analyse
DE102012205171B3 (de) Integriertes Einweg-Chipkartuschensystem für mobile Multiparameteranalysen chemischer und/oder biologischer Substanzen
DE60031526T2 (de) Durchdringbare kappe mit innerer spitze
EP1796838B1 (de) Verfahren zur durchführung einer elektrochemischen messung an einer flüssigen messprobe in einer über leitungen zugänglichen messkammer
DE10111457B4 (de) Diagnoseeinrichtung
EP2654955B1 (de) Verfahren zum mischen wenigstens einer probenlösung mit reagenzien
DE102008025992B4 (de) Titerplatte und Verfahren zur Detektion eines Analyten
DE19546535C2 (de) Meßkartusche für flüssige oder gasförmige Proben, Verfahren zu deren Betreiben und deren Verwendung
EP1752755B1 (de) Probenaufnahme- und-dosiervorrichtung mit integrierten Flüssigkeitskompartimenten
EP1160573A2 (de) Mikrotiterplatte und gekoppeltes Vielfachpipettiergerät
EP1883474A1 (de) System zur integrierten und automatisierten dna- oder protein-analyse und betriebsverfahren eines solchen systems
DE102004023217A1 (de) Chemisches Reaktionsmodul, dessen Herstellungsverfahren und Antriebssystem für chemisches Reaktionsmodul
EP1740722A1 (de) Verfahren und anordnung zur dna-isolierung mit trockenreagenzien
EP3108962B1 (de) Probenträger
EP3793736A1 (de) Fluidisches system zur aufnahme, abgabe und bewegung von flüssigkeiten, verfahren zur verarbeitung von fluiden in einem fluidischen system
DE602004009775T2 (de) Vorrichtung zur zuverlässige Analyse
EP1641564B1 (de) Verwendung eines einwegbehälters, mikrofluidische vorrichtung und verfahren zur bearbeitung von molekülen
DE102005049976A1 (de) Anordnung zur integrierten und automatisierten DNA- oder Protein-Analyse in einer einmal verwendbaren Cartridge, Herstellungsverfahren für eine solche Cartridge und Betriebsverfahren der DNA- oder Protein-Analyse unter Verwendung einer solchen Cartridge
DE102013201505A1 (de) Vorrichtung zur Extraktion von trocken vorgelagerter Körperflüssigkeit in einer Probe, Kartusche sowie Verfahren
EP2243024B1 (de) Einrichtung und verfahren zum nachweis von flüssigkeiten oder substanzen aus flüssigkeiten
DE102018102383B4 (de) System zur Durchführung biologischer oder chemischer Verfahren
EP2405802B1 (de) Probenahmevorrichtung und -verfahren
EP2959297B1 (de) Neues poc-testsystem und verfahren
EP1490673A2 (de) Vorrichtung und verfahren zur durchführung und zur kontinuierlichen überwachung von chemischen und/oder biologischen reaktionen
EP4446007A1 (de) Chromatographisches schnelltest-set zur untersuchung einer probe hinsichtlich pathogenen, biomolekülen, biomarkern und chemischen stoffen

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20120308

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

DAX Request for extension of the european patent (deleted)
RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: SIEMENS AKTIENGESELLSCHAFT

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: BOEHRINGER INGELHEIM VETMEDICA GMBH

17Q First examination report despatched

Effective date: 20161004

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20170411

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK SM TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

Free format text: LANGUAGE OF EP DOCUMENT: GERMAN

REG Reference to a national code

Ref country code: AT

Ref legal event code: REF

Ref document number: 918544

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170915

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 8

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502010014023

Country of ref document: DE

REG Reference to a national code

Ref country code: NL

Ref legal event code: MP

Effective date: 20170816

REG Reference to a national code

Ref country code: LT

Ref legal event code: MG4D

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: NO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171116

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171216

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171117

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20171116

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

REG Reference to a national code

Ref country code: CH

Ref legal event code: PL

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502010014023

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MC

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: IT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: SM

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

REG Reference to a national code

Ref country code: BE

Ref legal event code: MM

Effective date: 20170930

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170927

26N No opposition filed

Effective date: 20180517

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170927

Ref country code: LI

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170930

Ref country code: CH

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170930

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

Ref country code: BE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170930

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 9

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

REG Reference to a national code

Ref country code: AT

Ref legal event code: MM01

Ref document number: 918544

Country of ref document: AT

Kind code of ref document: T

Effective date: 20170927

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170927

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT; INVALID AB INITIO

Effective date: 20100927

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: CY

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170816

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: AL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20170816

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20210921

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20210920

Year of fee payment: 12

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20220620

Year of fee payment: 13

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20220927

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20220930

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20220927

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 502010014023

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20240403