EP2297296A2 - Bioreaktor mit doppelkammer - Google Patents
Bioreaktor mit doppelkammerInfo
- Publication number
- EP2297296A2 EP2297296A2 EP09765987A EP09765987A EP2297296A2 EP 2297296 A2 EP2297296 A2 EP 2297296A2 EP 09765987 A EP09765987 A EP 09765987A EP 09765987 A EP09765987 A EP 09765987A EP 2297296 A2 EP2297296 A2 EP 2297296A2
- Authority
- EP
- European Patent Office
- Prior art keywords
- cell
- compartments
- filter
- bioreactor
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 230000009977 dual effect Effects 0.000 title claims description 3
- 238000000034 method Methods 0.000 claims abstract description 20
- 230000029087 digestion Effects 0.000 claims abstract description 10
- 238000010494 dissociation reaction Methods 0.000 claims abstract description 10
- 230000005593 dissociations Effects 0.000 claims abstract description 10
- 238000005406 washing Methods 0.000 claims abstract description 7
- 210000004153 islets of langerhan Anatomy 0.000 claims description 6
- 239000006285 cell suspension Substances 0.000 claims description 5
- 238000004113 cell culture Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 102000008186 Collagen Human genes 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 238000003501 co-culture Methods 0.000 claims description 2
- 229920001436 collagen Polymers 0.000 claims description 2
- 230000001131 transforming effect Effects 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims 1
- 102000029816 Collagenase Human genes 0.000 abstract 1
- 108060005980 Collagenase Proteins 0.000 abstract 1
- 229960002424 collagenase Drugs 0.000 abstract 1
- 238000004898 kneading Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 9
- 239000011324 bead Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000007654 immersion Methods 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 229910001220 stainless steel Inorganic materials 0.000 description 2
- 239000010935 stainless steel Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 229920002457 flexible plastic Polymers 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 210000004923 pancreatic tissue Anatomy 0.000 description 1
- 229920002946 poly[2-(methacryloxy)ethyl phosphorylcholine] polymer Polymers 0.000 description 1
- 229920000306 polymethylpentene Polymers 0.000 description 1
- 239000011116 polymethylpentene Substances 0.000 description 1
- 230000028016 temperature homeostasis Effects 0.000 description 1
- 230000000472 traumatic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/08—Chemical, biochemical or biological means, e.g. plasma jet, co-culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Definitions
- the present invention relates to a dual-compartment bioreactor capable of allowing transformation of a tissue or cell product, and a method for its implementation.
- the devices currently used in an application to the extraction of islets of Langerhans consist of hard-walled chambers.
- the present invention proposes to remedy the first two disadvantages presented by proposing a technique whose basic idea is to use a separation chamber of flexible material that allows an operator to perform tissue dissociation by external manual manipulations in aseptic conditions and possibly make possible a thermoregulation of the product by immersion in a liquid taking into account the tightness of the device.
- the invention thus relates to a dual compartment bioreactor, characterized in that it comprises a flexible bag separated into two compartments separated by a filter, and at least one inlet opening in communication with one of the compartments and at least one exit opening in communication with the other compartment.
- At least one of the compartments may have both an inlet opening and an outlet opening.
- the invention also relates to a method of using such a bioreactor, characterized in that it implements the implementation in a biological treatment apparatus including washing or transformation of a tissue or cell product, the circulating a solution between an inlet opening and an outlet opening of the pocket. More particularly, the invention relates to a method which implements, for tissue dissociation, in particular the sorting of islets of Langerhans, the establishment of said bioreactor as a digestion chamber and a manual mixing of the flexible bag, to achieve said dissociation, in particular for separating islands of Langerhans, during a digestion step during which a collagen-containing digestion solution circulates between inlet and outlet openings of the pocket.
- tissue dissociation in particular the sorting of islets of Langerhans
- the establishment of said bioreactor as a digestion chamber and a manual mixing of the flexible bag
- FIG. 1a represents a flexible bag that can be used as a sterile disposable bioreactor for a process for transforming a tissue or cell product, of which FIG. 1b is a section along AA.
- the flexible pouch 1 of flexible plastic material has two compartments 2 and 3 separated by a filter 4 fixed in sagittal position.
- the flexible bag 1 may be similar to a blood bag, and the filter 4 has a periphery 7 which is welded to position the filter 4 in the sagittal position in the bag 1.
- the size of the mesh of the filter 4 is a function of the application assigned to the device.
- the introduction, the renewal and the circulation of the fluids or the biological material inside the device, are carried out through entry access and exit end pieces 6 opening on each of the compartments 2 and 3 of the pocket.
- the system is thus integrable with a conventional equipment or circuit.
- the tightness of the device allows its complete immersion in a liquid medium (thermal regulation), as well as an aseptic.
- the invention applies to tissue dissociation, for example islet extraction of Langerhans with in this case a filter mesh of between 50 .mu.m and 600 .mu.m or for use in biological reactions, for example cell culture, co- culture, or else tissue engineering or as a device for washing tissues or cell suspensions, without the need to centrifuge the cell suspension, especially with cell cultures or washing cell suspensions, a filter size n authorizing the passage of an acellular fluid (without cells).
- tissue dissociation for example islet extraction of Langerhans with in this case a filter mesh of between 50 .mu.m and 600 .mu.m or for use in biological reactions, for example cell culture, co- culture, or else tissue engineering or as a device for washing tissues or cell suspensions, without the need to centrifuge the cell suspension, especially with cell cultures or washing cell suspensions, a filter size n authorizing the passage of an acellular fluid (without cells).
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Clinical Laboratory Science (AREA)
- General Chemical & Material Sciences (AREA)
- Physiology (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0802824A FR2931484B1 (fr) | 2008-05-26 | 2008-05-26 | Bioreacteur a double compartiment. |
| PCT/FR2009/000550 WO2009153425A2 (fr) | 2008-05-26 | 2009-05-13 | Bioreacteur a double compartiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| EP2297296A2 true EP2297296A2 (de) | 2011-03-23 |
Family
ID=40303448
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP09765987A Withdrawn EP2297296A2 (de) | 2008-05-26 | 2009-05-13 | Bioreaktor mit doppelkammer |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20110189772A1 (de) |
| EP (1) | EP2297296A2 (de) |
| FR (1) | FR2931484B1 (de) |
| WO (1) | WO2009153425A2 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8603805B2 (en) | 2005-04-22 | 2013-12-10 | Hyclone Laboratories, Inc. | Gas spargers and related container systems |
| WO2011025890A1 (en) * | 2009-08-26 | 2011-03-03 | Xcellerex, Inc. | Continuous recovery harvest bag |
| US9376655B2 (en) | 2011-09-29 | 2016-06-28 | Life Technologies Corporation | Filter systems for separating microcarriers from cell culture solutions |
| JP6101698B2 (ja) | 2011-09-30 | 2017-03-22 | ライフ テクノロジーズ コーポレイション | フィルムスパージャを有する容器 |
| US9079690B1 (en) | 2014-06-26 | 2015-07-14 | Advanced Scientifics, Inc. | Freezer bag, storage system, and method of freezing |
| CN104893975B (zh) * | 2015-05-28 | 2016-06-01 | 深圳富利鑫健康产业发展有限公司 | 一种双室选择透过性细胞培养装置及其应用 |
| US10589197B2 (en) | 2016-12-01 | 2020-03-17 | Life Technologies Corporation | Microcarrier filter bag assemblies and methods of use |
| JP6803221B2 (ja) * | 2016-12-26 | 2020-12-23 | 東洋製罐グループホールディングス株式会社 | フィルタ付き細胞用バッグ、及びその製造方法 |
| JP6795765B2 (ja) * | 2017-03-29 | 2020-12-02 | 公立大学法人秋田県立大学 | 細胞膜穿孔用容器およびこれを用いた細胞膜穿孔方法 |
| CN109294869B (zh) * | 2018-10-18 | 2021-07-27 | 上海蓓蕊医疗科技有限公司 | 一种袋式过滤器、脂肪提取系统及其操作方法 |
| WO2021114087A1 (zh) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | 小体积细胞培养袋 |
| EP4150045A1 (de) * | 2020-05-12 | 2023-03-22 | PBS Biotech, Inc. | Materialübertragungsvorrichtungen sowie entsprechende systeme und verfahren |
| EP4225885A1 (de) * | 2020-10-05 | 2023-08-16 | Scinus Holding B.V. | Zellkulturbeutel, bioreaktor und zellfiltrationsvorrichtung |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5714384A (en) * | 1994-06-28 | 1998-02-03 | Wilson; John R. | Compartmentalized tissue culture bag |
| EP0725134A3 (de) * | 1995-02-03 | 1998-05-13 | NPBI Nederlands Produktielaboratorium voor Bloedtransfusieapparatuur en Infusievloeistoffen B.V. | Flexibler Bioreaktor für therapeutische Zellen |
| FR2797887B1 (fr) * | 1999-08-25 | 2002-01-25 | Rech S Tech Modernes S A | Dispositif emboitable a usage biologique, notamment pour la culture cellulaire |
| US6759039B2 (en) * | 2000-06-30 | 2004-07-06 | Amcyte, Inc. | Culturing pancreatic stem cells having a specified, intermediate stage of development |
| ITMI20031191A1 (it) * | 2003-06-13 | 2004-12-14 | Elep Sas Di Cabiddu Rachele & C | Dispositivo ed impianto per fermentazioni in stato solido |
-
2008
- 2008-05-26 FR FR0802824A patent/FR2931484B1/fr not_active Expired - Fee Related
-
2009
- 2009-05-13 EP EP09765987A patent/EP2297296A2/de not_active Withdrawn
- 2009-05-13 US US12/994,582 patent/US20110189772A1/en not_active Abandoned
- 2009-05-13 WO PCT/FR2009/000550 patent/WO2009153425A2/fr not_active Ceased
Non-Patent Citations (1)
| Title |
|---|
| See references of WO2009153425A2 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009153425A3 (fr) | 2010-09-30 |
| FR2931484A1 (fr) | 2009-11-27 |
| WO2009153425A2 (fr) | 2009-12-23 |
| FR2931484B1 (fr) | 2011-01-07 |
| US20110189772A1 (en) | 2011-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| WO2009153425A2 (fr) | Bioreacteur a double compartiment | |
| JP3041641B2 (ja) | 多数の試料を収集し培養し次いで濾過するモジュール装置 | |
| KR101910685B1 (ko) | 세포 수집 방법 및 시스템 | |
| CN103805511B (zh) | 可在高倍物镜下直接观测的动脉血管模拟微流控装置 | |
| JP2017513512A (ja) | 自動細胞培養及び回収装置 | |
| US20140315303A1 (en) | Method and device for sample processing | |
| US11884905B2 (en) | Fluidic chip for cell culture use, culture vessel, and culture method | |
| WO2013086199A1 (en) | Method and device for isolation of non-fat cells from an adipose tissue | |
| JP5049898B2 (ja) | 生体物質の洗浄装置及び方法 | |
| JP2016202180A (ja) | 細胞培養器、及び、細胞培養システム | |
| CN101960004A (zh) | 非侵入性自动细胞增殖装置 | |
| EP1903101B1 (de) | Verfahren zur Erneuerung eines biologischen Mediums, in dem Zellen in Suspension sind | |
| JP2011244713A (ja) | 細胞培養デバイス、細胞培養システム、及び細胞培養方法 | |
| US20130344579A1 (en) | Imaging chamber for supporting multiple investigation of cells and tissues by various techniques | |
| US9101924B2 (en) | Interface device for bio-chip | |
| CA2186641C (fr) | Dispositif de filtration de fluides biologiques et son application | |
| JP2006314204A (ja) | 培養容器からの動物細胞の剥離方法および剥離回収方法 | |
| US20150343351A1 (en) | Multifunctional System for Particle Separation | |
| JP7039202B2 (ja) | 液体受容空間を具備する脆弱物保持デバイス | |
| JPWO2020003504A1 (ja) | 灌流培養システム | |
| WO2019059279A1 (ja) | 細胞懸濁液を調製するためのデバイス、システム及び方法 | |
| JP6732245B2 (ja) | 細胞培養装置 | |
| FR2797887A1 (fr) | Dispositif emboitable a usage biologique, notamment pour la culture cellulaire | |
| JP5167898B2 (ja) | サンプリング装置およびこれを用いたサンプリング方法 | |
| FR2786783A1 (fr) | Poche comportant un textile poreux biocompatible pour l'expansion tridimensionnelle in vitro de cellules notamment a usage therapeutique |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 20101221 |
|
| AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR |
|
| RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: LARGHERO, JEROME Inventor name: CATTAN, PIERRE Inventor name: BENBUNAN, MARC |
|
| DAX | Request for extension of the european patent (deleted) | ||
| GRAP | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOSNIGR1 |
|
| INTG | Intention to grant announced |
Effective date: 20141007 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
| 18D | Application deemed to be withdrawn |
Effective date: 20150218 |