EP2188360A1 - Composés polycycliques comme stabilisateurs d'enzymes - Google Patents

Composés polycycliques comme stabilisateurs d'enzymes

Info

Publication number
EP2188360A1
EP2188360A1 EP08803298A EP08803298A EP2188360A1 EP 2188360 A1 EP2188360 A1 EP 2188360A1 EP 08803298 A EP08803298 A EP 08803298A EP 08803298 A EP08803298 A EP 08803298A EP 2188360 A1 EP2188360 A1 EP 2188360A1
Authority
EP
European Patent Office
Prior art keywords
protease
washing
cleaning agent
agent according
alkaline protease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08803298A
Other languages
German (de)
English (en)
Inventor
Robin Ghosh
Andreas Michels
Cornelius Bessler
Daniela Lowis
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Henkel AG and Co KGaA
Original Assignee
Henkel AG and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA filed Critical Henkel AG and Co KGaA
Publication of EP2188360A1 publication Critical patent/EP2188360A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/26—Organic compounds containing nitrogen
    • C11D3/33—Amino carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/20—Organic compounds containing oxygen
    • C11D3/2075—Carboxylic acids-salts thereof
    • C11D3/2089—Ether acids-salts thereof
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/20—Organic compounds containing oxygen
    • C11D3/2096—Heterocyclic compounds
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/26—Organic compounds containing nitrogen
    • C11D3/28—Heterocyclic compounds containing nitrogen in the ring
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38663—Stabilised liquid enzyme compositions

Definitions

  • the present invention relates to detergents and cleaners containing polycyclic compounds which act as protease inhibitors and are thus suitable enzyme stabilizers.
  • enzymes in detergents and cleaners have been established in the art for decades. They serve to extend the range of services of the funds concerned according to their specific activities. These include in particular hydrolytic enzymes such as proteases, amylases, lipases and cellulases. The first three hydrolyze proteins, starches and fats and thus contribute directly to soil removal. Cellulases are used in particular because of their tissue effect.
  • Another group of washing and cleaning agent enzymes are oxidative enzymes, in particular oxidases, which, if appropriate, in combination with other components, are preferably used to bleach soiling or to produce the bleaching agents in situ.
  • enzymes which are subjected to constant optimization, further enzymes are constantly being made available for use in detergents and cleaners in order to be able to optimally address particular soiling, such as pectinases, ⁇ -glucanases, mannanases or other hemicellulases (glycosidases) Hydrolysis in particular of special vegetable polymers.
  • soiling such as pectinases, ⁇ -glucanases, mannanases or other hemicellulases (glycosidases) Hydrolysis in particular of special vegetable polymers.
  • One goal in the development of detergent formulations is to stabilize the enzymes contained, especially during storage. This is understood as protection against various unfavorable influences, such as denaturation or decay by physical influences or oxidation.
  • One focus of these developments is the protection of the contained proteins and / or enzymes against proteolytic cleavage. This can be done by building physical barriers, such as by encapsulating the Enzymes in special enzyme granules or by compounding the means in two- or multi-chamber systems.
  • the other many pathway is to add chemical compounds that inhibit the proteases and thus act collectively as stabilizers for proteases and the other proteins and enzymes contained. It must be reversible protease inhibitors, since the protease activity only temporarily, especially during the storage, but not be suppressed during the cleaning process.
  • Polyols in particular glycerol and 1,2-propylene glycol, benzamidine hydrochloride, borax, boric acids, boronic acids or their salts or esters are established as reversible protease inhibitors in the prior art.
  • These include, in particular, derivatives having aromatic groups, for example ortho, meta or para-substituted phenylboronic acids, in particular 4-formylphenylboronic acid, or the salts or esters of the abovementioned compounds (see above).
  • a particularly good protection results when boric acid derivatives are used together with polyols, since they can then form a complex stabilizing the enzyme.
  • peptide aldehydes that is, oligopeptides with reduced C-terminus, especially those of 2 to 50 monomers are described for this purpose.
  • peptidic reversible protease inhibitors include ovomucoid and leupeptin.
  • specific, reversible peptide inhibitors and fusion proteins from proteases and specific peptide inhibitors are used for this purpose.
  • More established enzyme stabilizers are aminoalcohols, such as mono-, di-, triethanol- and - propanolamine and mixtures thereof, aliphatic carboxylic acids up to Ci 2, such as succinic acid, other dicarboxylic acids or salts of said acids. End-capped fatty acid amide alkoxylates are also established for this purpose. Certain organic acids used as builders are capable, as disclosed in WO 97/18287, of stabilizing an enzyme in addition to their builder function.
  • subtilisin-type proteases (subtilases, subtilopeptidases, EC 3.4.21.62) occupy an outstanding position due to their favorable enzymatic properties such as stability or pH optimum. They are attributed to the serine proteases due to the catalytically active amino acids. They act as nonspecific endopeptidases, that is, they hydrolyze any acid amide linkages that are internal to peptides or proteins. Their pH optimum is usually in the clearly alkaline range. For an overview of this family, see for example the article "Subtilases: Subtilisin-like Proteases" by R.
  • Subtilisin enzymes edited by R. Bott and C. Betzel, New York, 1996.
  • Subtilases become natural formed by microorganisms; These include in particular those of To mention Bacillus species formed and secreted subtilisins as the most significant group within the subtilases.
  • WO 96/21716 A1 cites the five applications cited above and discloses that all the protease inhibitors listed therein are also suitable for the specific purpose of stabilizing enzymes in detergents and cleaners. A selection of particularly efficient stabilizers thereof discloses WO 96/41859 A1.
  • X and Y independently of one another represent O, NR 1 or CR 1 R 2 , preferably O,
  • R 1, R 2 and Z are independently hydrogen, Ci -6 alkyl, especially methyl, ethyl,
  • Ci -6 alkenyl, in particular ethenyl, propenyl, butenyl, pentenyl or hexenyl, phenyl, benzyl or halogen, in particular fluorine, chlorine, bromine or iodine, preferably hydrogen, are n 1 or 2.
  • a detergent or cleaning agent according to the invention are all means that are suitable for washing or cleaning of particular textiles and / or solid surfaces. Suitable ingredients for this purpose are detailed below.
  • a protease is to be understood as meaning all enzymes which are capable of hydrolyzing acid amide linkages of proteins.
  • the proteases are also detailed below.
  • the first advantage of the compounds relevant to the invention over the prior art, in addition to their lower volume requirement compared to the polyols, is that they provide favorable inhibition constants with respect to the proteases which can be used in detergents and cleaners exhibit. This applies, for example, to serine proteases, but also to metalloproteases.
  • the inhibitors thus bind reversibly, ie they do not undergo solid and not too loose transient interactions with the enzyme.
  • the majority of the protease relevant to the invention is thus present during storage in the form of a protease-inhibitor complex.
  • the protease and possibly other proteins contained, in particular other enzymes are protected in this way against proteolysis by this enzyme (stabilized against proteolysis).
  • a washing or cleaning process in which a protease is inhibited which is inhibited and / or stabilized with a compound described above;
  • those detergents or cleaners are preferred in which the stabilizing compound has an inhibition constant (Ki) of 0.01 to 10 mM, preferably 0.1 to 5, particularly preferably 0.5 to 2, with respect to the protease contained.
  • Ki inhibition constant
  • the inhibition constant K can be determined in the following way:
  • the inhibition constant K 1 is a characteristic and decisive variable.
  • K 1 describes the equilibrium between enzyme, inhibitor and enzyme-inhibitor complex for reversible binding.
  • the enzyme-inhibitor complex is catalytically inactive and inhibits the reaction by reducing the concentration of free enzyme that is still available for binding of substrate.
  • the ki is accordingly defined as:
  • [E], [I] and [El] denote the respective molar equilibrium concentrations of enzyme (E), inhibitor (I) and the enzyme-inhibitor complex (El). According to this definition, a substance with a small Ki is a good inhibitor under the respective test conditions.
  • K 1 can be determined using the Cheng-Prusoff equation (Equation 2) via the IC 50 value.
  • the determination of the IC 50 value is made by determining the proteolytic activity on a substrate in the presence of various concentrations of the inhibitor and fitting the experimental data to a variable slope sigmoid dose-response equation (pseudo-hill slopes). It is the value of half the inhibitor concentration that would be needed to achieve complete inhibition.
  • [S] is the substrate concentration in the assay and K d is the equilibrium dissociation constant for the substrate, which in IC 50 can be set to be identical to K m for the substrate.
  • the protease is especially in a content of 2 .mu.g to 20 mg per g of the composition, preferably from 5 ⁇ g to 17.5 mg per g of the agent, more preferably from 20 ⁇ g to 15 mg per g of the agent, most preferably from 50 ⁇ g to 10 ⁇ g of the agent.
  • the molar ratio of stabilizer to protease is preferably in the range from 1: 1 to 1000: 1, in particular from 1: 1 to 500: 1, particularly preferably from 1: 1 to 100: 1, very particularly preferably from 1: 1 to 20 :1.
  • an agent according to the invention may contain at least one further stabilizer, in particular a polyol, such as glycerol or 1,2-ethylene glycol, and / or an antioxidant.
  • a further stabilizer in particular a polyol, such as glycerol or 1,2-ethylene glycol, and / or an antioxidant.
  • the protease stabilized or reversibly inhibited according to the invention is preferably a serine protease, in particular a subtilase, more preferably a subtilisin.
  • Subtilisin may be a wild-type enzyme or a subtilisin variant, wherein the wild-type enzyme or the starting enzyme of the variant is preferably selected from one of the following:
  • the alkaline protease from Bacillus lentus preferably from Bacillus lentus (DSM 5483),
  • the alkaline protease from Bacillus alcalophilus (DSM 11233),
  • the alkaline protease from Bacillus gibsonii (DSM 14391) or an at least 70% identical alkaline protease, the alkaline protease from Bacillus sp. (DSM 14390) or an at least 98.5% identical alkaline protease, the alkaline protease from Bacillus sp. (DSM 14392) or an at least 98.1% identical alkaline protease,
  • the protease is a variant with a point mutation in the range of positions 95 to 103 (counting according to subtilisin 309), preferably with an insertion of a single amino acid between position 99 and 100, more preferably starting from subtilisin 147 and / or 309 (subtilisin 309), or a variant thereof.
  • the protease is a variant with a point mutation in position 217 (count according to the wild-type protease from Bacillus amyloliquefaciens, BPN '), preferably with a substitution of a single amino acid in this position, more preferably with the amino acid substitution X217L, most preferably starting from the wild type protease from Bacillus amyloliquefaciens (BPN '), or a variant thereof.
  • the protease is a variant having an amino acid change relative to a starting protease homologizable with the Bacillus lentus alkaline protease in one or more of the following positions: 3, 4, 36, 42, 43, 47, 56 , 61, 69, 87, 96, 99, 101, 102, 104, 114, 1, 18, 120, 130, 139, 141, 142, 154, 157, 188, 193, 199, 205, 211, 224, 229, 236, 237, 242, 243, 250, 253, 255 and 268, in the count the Bacillus lentus alkaline protease, preferably with an amino acid change from the parent molecule in one or more of the following positions: 3, 4, 43, 61, 188, 193, 199, 21 1, 224, 250 and 253, most preferably with one or more several of the amino acid exchanges X3T, X4I, X43V, X61A, X
  • Agents according to the invention may contain, in addition to the protease, one or more further enzymes, in particular from the following group: one or more further proteases, amylases, hemicellulases, cellulases, lipases and oxidoreductases.
  • the amylase is preferably an ⁇ -amylase.
  • the hemicellulase is preferably a ⁇ -glucanase, a pectinase, a pullulanase and / or a mannanase.
  • the cellulase is preferably a cellulase mixture or a one-component cellulase, preferably or predominantly an endoglucanase and / or a cellobiohydrolase.
  • the oxidoreductase is preferably an oxidase, in particular a choline oxidase, or a perhydrolase.
  • Agents according to the invention preferably comprise at least one complexing agent and / or builder substances, the builder being in particular a zeolite builder, and / or a nonionic surfactant, the nonionic surfactant preferably being a hydroxy mixed ether, and / or optical Brightener, wherein the optical brightener is diphenyl compounds, in particular distyryl biphenyl derivatives, and / or stilbentriazine derivatives.
  • the proteolytic residual activity of the Bacillus lentus alkaline protease F49 (according to WO 95/23221 A1) is determined in the presence of these compounds.
  • the basic formulation is a liquid detergent having the following composition (all figures in percentages by weight): 0.3-0.5% xanthan gum, 0.2-0.4% anti-foaming agent, 6-7% glycerol, 0.3 -0.5% ethanol, 4-7% FAEOS, 24-28% nonionic surfactants, 1% boric acid, 1-2% sodium citrate (dihydrate), 2-4% soda, 14-16% coconut fatty acids, 0.5 % HEDP, 0-0.4% PVP, 0-0.05% optical brightener, 0-0.001% dye, balance: demineralized water.
  • This formulation is supplemented with the inhibiting compounds to be tested and 1,275,000 HPE / I B. lentus alkaline protease F 49.
  • the protease activity (handle-protease units) indicated in HPE is according to van Raay, Saran and Verbeek, according to the publication "For the determination of the proteolytic activity in enzyme concentrates and enzyme-containing detergents, rinses and cleaners" in Tenside (1970), Volume 7, pp. 125-132.
  • the storage is carried out for various periods of time in airtight containers at 30 0 C.
  • the initial values for the proteolytic activity of the agent in question are compared with the values determined after storage.

Abstract

L'invention concerne des lessives et produits de nettoyage contenant des composés polycycliques qui agissent comme inhibiteurs de protéase et sont ainsi des stabilisateurs d'enzymes appropriés.
EP08803298A 2007-09-04 2008-08-28 Composés polycycliques comme stabilisateurs d'enzymes Withdrawn EP2188360A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102007041754A DE102007041754A1 (de) 2007-09-04 2007-09-04 Polycyclische Verbindungen als Enzymstabilisatoren
PCT/EP2008/061272 WO2009030632A1 (fr) 2007-09-04 2008-08-28 Composés polycycliques comme stabilisateurs d'enzymes

Publications (1)

Publication Number Publication Date
EP2188360A1 true EP2188360A1 (fr) 2010-05-26

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Family Applications (1)

Application Number Title Priority Date Filing Date
EP08803298A Withdrawn EP2188360A1 (fr) 2007-09-04 2008-08-28 Composés polycycliques comme stabilisateurs d'enzymes

Country Status (5)

Country Link
US (1) US20100210502A1 (fr)
EP (1) EP2188360A1 (fr)
JP (1) JP2010538138A (fr)
DE (1) DE102007041754A1 (fr)
WO (1) WO2009030632A1 (fr)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1807498B2 (fr) † 2004-11-02 2019-02-20 Henkel AG & Co. KGaA Procede de preparation des granules / agglomerats pour compositions detergentes
CN102906251B (zh) 2010-04-26 2016-11-16 诺维信公司 酶颗粒剂
DE102010028951A1 (de) * 2010-05-12 2011-11-17 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Lipase
DE102010063457A1 (de) * 2010-12-17 2012-06-21 Henkel Ag & Co. Kgaa Lagerstabiles flüssiges Wasch- oder Reinigungsmittel enthaltend Protease und Cellulase
US10829721B2 (en) 2011-06-20 2020-11-10 Novozymes A/S Particulate composition
CN103620029B (zh) 2011-06-24 2017-06-09 诺维信公司 具有蛋白酶活性的多肽和编码它们的多核苷酸
JP5829844B2 (ja) * 2011-06-24 2015-12-09 太邦株式会社 水溶性エラスチンペプチドの製造方法
US20140206026A1 (en) 2011-06-30 2014-07-24 Novozymes A/S Method for Screening Alpha-Amylases
EP2732018B1 (fr) 2011-07-12 2017-01-04 Novozymes A/S Granules d'enzyme stables au stockage
WO2013024021A1 (fr) 2011-08-15 2013-02-21 Novozymes A/S Polypeptides ayant une activité cellulase et polynucléotides codant pour ceux-ci
ES2628190T3 (es) 2011-09-22 2017-08-02 Novozymes A/S Polipéptidos con actividad de proteasa y polinucleótidos que codifican los mismos
DE102011118021A1 (de) * 2011-10-28 2013-05-02 Henkel Ag & Co. Kgaa Leistungsverbesserte und temperaturstabile Proteasevarianten
WO2013076269A1 (fr) 2011-11-25 2013-05-30 Novozymes A/S Variants de subtilase et polynucléotides codants pour ceux-ci
MX2014007446A (es) 2011-12-20 2014-08-01 Novozymes As Variantes de subtilasa y polinucleotidos que las codifican.
EP2807254B1 (fr) 2012-01-26 2017-08-02 Novozymes A/S Utilisation de polypeptides à activité protéasique dans les aliments pour animaux et les détergents
CN104114698A (zh) 2012-02-17 2014-10-22 诺维信公司 枯草杆菌蛋白酶变体以及编码它们的多核苷酸
EP2823026A1 (fr) 2012-03-07 2015-01-14 Novozymes A/S Composition détergente et substitution d'azurants optiques dans des compositions détergentes
AR090971A1 (es) 2012-05-07 2014-12-17 Novozymes As Polipeptidos que tienen actividad de degradacion de xantano y polinucleotidos que los codifican
MX2014015186A (es) * 2012-06-13 2015-09-25 Novozymes As Barras de jabon para ropa.
MX364390B (es) 2012-06-20 2019-04-25 Novozymes As Uso de polipeptidos que tienen actividad proteasa en alimentos para animales y detergentes.
ES2655032T3 (es) 2012-12-21 2018-02-16 Novozymes A/S Polipéptidos que poseen actividad proteasa y polinucleótidos que los codifican
EP3321360A3 (fr) 2013-01-03 2018-06-06 Novozymes A/S Variants d'alpha-amylase et polynucléotides les codant
EP2997143A1 (fr) 2013-05-17 2016-03-23 Novozymes A/S Polypeptides présentant une activité alpha-amylase
EP3786269A1 (fr) 2013-06-06 2021-03-03 Novozymes A/S Variants d'alpha-amylase et polynucléotides les codant
EP3013955A1 (fr) 2013-06-27 2016-05-04 Novozymes A/S Variants de subtilase et polynucléotides codant pour ceux-ci
WO2014207227A1 (fr) 2013-06-27 2014-12-31 Novozymes A/S Variants de subtilase et polynucléotides codant pour ceux-ci
US20160152925A1 (en) 2013-07-04 2016-06-02 Novozymes A/S Polypeptides Having Anti-Redeposition Effect and Polynucleotides Encoding Same
EP2832853A1 (fr) 2013-07-29 2015-02-04 Henkel AG&Co. KGAA Composition détergente comprenant des variantes de protéases
EP3027747B1 (fr) 2013-07-29 2018-02-07 Novozymes A/S Variants de protéases et polynucléotides les codant
CN117904081A (zh) 2013-07-29 2024-04-19 诺维信公司 蛋白酶变体以及对其进行编码的多核苷酸
WO2015049370A1 (fr) 2013-10-03 2015-04-09 Novozymes A/S Composition détergente et utilisation de celle-ci
EP3453757B1 (fr) 2013-12-20 2020-06-17 Novozymes A/S Polypeptides a activite de protease et polynucleotides les codant
EP3114272A1 (fr) 2014-03-05 2017-01-11 Novozymes A/S Compositions et procédés pour améliorer les propriétés de matières textiles cellulosiques avec une xyloglucane endotransglycosylase
WO2015134729A1 (fr) 2014-03-05 2015-09-11 Novozymes A/S Compositions et procédés destinés à améliorer les propriétés de matériaux textiles non-cellulosiques par l'utilisation d'endo-xyloglucane transférase
US20170015950A1 (en) 2014-04-01 2017-01-19 Novozymes A/S Polypeptides having alpha amylase activity
US20170121695A1 (en) 2014-06-12 2017-05-04 Novozymes A/S Alpha-amylase variants and polynucleotides encoding same
EP3739029A1 (fr) 2014-07-04 2020-11-18 Novozymes A/S Variants de subtilase et polynucléotides codant pour ceux-ci
CN106661566A (zh) 2014-07-04 2017-05-10 诺维信公司 枯草杆菌酶变体以及编码它们的多核苷酸
US10287562B2 (en) 2014-11-20 2019-05-14 Novoszymes A/S Alicyclobacillus variants and polynucleotides encoding same
DE102014224746A1 (de) * 2014-12-03 2016-06-09 Henkel Ag & Co. Kgaa Enzymstabilisatoren
EP3227444B1 (fr) 2014-12-04 2020-02-12 Novozymes A/S Variants de subtilase et polynucléotides codant pour ceux-ci
EP3234121A1 (fr) 2014-12-15 2017-10-25 Henkel AG & Co. KGaA Composition de détergent comprenant des variants de subtilase
US11162089B2 (en) 2015-06-18 2021-11-02 Novozymes A/S Subtilase variants and polynucleotides encoding same
EP3106508B1 (fr) 2015-06-18 2019-11-20 Henkel AG & Co. KGaA Composition détergente comprenant des variantes de subtilase
EP4324919A2 (fr) 2015-10-14 2024-02-21 Novozymes A/S Variants polypeptidiques
CN108291215A (zh) 2015-10-14 2018-07-17 诺维信公司 具有蛋白酶活性的多肽以及编码它们的多核苷酸
DE102015223268A1 (de) * 2015-11-25 2017-06-01 Henkel Ag & Co. Kgaa Enzymstabilisatoren
DE102015223563A1 (de) * 2015-11-27 2017-06-01 Henkel Ag & Co. Kgaa Enzymstabilisatoren
CA3024276A1 (fr) 2016-06-03 2017-12-07 Novozymes A/S Variants de subtilase et polynucleotides codant pour ceux-ci
DE102016211115A1 (de) * 2016-06-22 2017-12-28 Henkel Ag & Co. Kgaa Enzymstabilisatoren
WO2018011276A1 (fr) 2016-07-13 2018-01-18 The Procter & Gamble Company Variants dnase de bacillus cibi et leurs utilisations
HUE057471T2 (hu) 2017-10-27 2022-05-28 Procter & Gamble Polipeptid-variánsokat tartalmazó mosószerkészítmények
EP3701016A1 (fr) 2017-10-27 2020-09-02 Novozymes A/S Variants de la dnase
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EP3942032A1 (fr) 2019-03-21 2022-01-26 Novozymes A/S Variants d'alpha-amylase et polynucléotides codant pour ceux-ci
WO2020207944A1 (fr) 2019-04-10 2020-10-15 Novozymes A/S Variants polypeptidiques
CN114787329A (zh) 2019-08-27 2022-07-22 诺维信公司 洗涤剂组合物
EP4031644A1 (fr) 2019-09-19 2022-07-27 Novozymes A/S Composition détergente
US20220340843A1 (en) 2019-10-03 2022-10-27 Novozymes A/S Polypeptides comprising at least two carbohydrate binding domains
EP3892708A1 (fr) 2020-04-06 2021-10-13 Henkel AG & Co. KGaA Compositions de nettoyage comprenant des variantes de dispersine
CN116507725A (zh) 2020-10-07 2023-07-28 诺维信公司 α-淀粉酶变体
CN116829709A (zh) 2021-02-12 2023-09-29 诺维信公司 α-淀粉酶变体
EP4359518A1 (fr) 2021-06-23 2024-05-01 Novozymes A/S Polypeptides d'alpha-amylase

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4028263A (en) * 1973-08-24 1977-06-07 Colgate-Palmolive Company Bleaching and brightening detergent composition
JPH02304080A (ja) * 1989-05-17 1990-12-17 Toyo Pharma- Kk ６Ｈ―ジベンゾ［ｂ，ｄ］ピラン―６―オン誘導体，その製法及び用途
NZ237570A (en) 1990-04-13 1993-09-27 Colgate Palmolive Co Enzyme stabilising composition and stabilised enzyme-containing built detergent compositions
EP0478050A1 (fr) 1990-09-24 1992-04-01 Unilever N.V. Composition détergente
EP0583383B1 (fr) 1991-04-30 1995-12-13 The Procter & Gamble Company Detergents liquides comprenant un acide boronique aryle
EP0511456A1 (fr) 1991-04-30 1992-11-04 The Procter & Gamble Company Détergents liquides contenant un ester aromatique de l'acide borique pour inhibition d'enzyme protéolitique
TW232026B (fr) 1991-12-04 1994-10-11 Procter & Gamble
CA2166683A1 (fr) 1993-07-09 1995-01-19 Lone Kierstein Nielsen Derives d'acides du bore, stabilisateurs d'enzymes
DE69535736T2 (de) 1994-02-24 2009-04-30 Henkel Ag & Co. Kgaa Verbesserte enzyme und diese enthaltene detergentien
US5834415A (en) 1994-04-26 1998-11-10 Novo Nordisk A/S Naphthalene boronic acids
JPH10511855A (ja) 1995-01-09 1998-11-17 ノボ ノルディスク アクティーゼルスカブ 液体酵素組成物の安定化
BR9608857A (pt) 1995-06-13 1999-06-15 Novo Nordisk As Composição líquida e detergente líquida
US5741767A (en) 1995-11-16 1998-04-21 Lever Brothers Company, Division Of Conopco, Inc. Peracid based dishwashing detergent composition
US6214596B1 (en) * 1996-12-18 2001-04-10 Alcon Laboratories, Inc. Liquid enzyme compositions and methods of use in contact lens cleaning and disinfecting systems
GB0022961D0 (en) * 2000-09-19 2000-11-01 Zeneca Ltd Fungicidal coumarin derivatives
DE10340683A1 (de) * 2003-09-04 2005-04-07 Henkel Kgaa Wasch- oder Reinigungsmittel
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
DE102004019751A1 (de) * 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
WO2006007864A1 (fr) * 2004-07-17 2006-01-26 Max Planck Geselllschaft Zur Förderung Der Wissenschaft Traitement d'etats neurodegeneratifs
DE102005028295A1 (de) 2005-06-18 2006-11-16 Henkel Kgaa Proteasen aus psychrophilen Organismen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2009030632A1 *

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JP2010538138A (ja) 2010-12-09
DE102007041754A1 (de) 2009-03-05
US20100210502A1 (en) 2010-08-19

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