EP2027264A4 - Véhicule d'expression multigénique - Google Patents

Véhicule d'expression multigénique

Info

Publication number
EP2027264A4
EP2027264A4 EP07718958A EP07718958A EP2027264A4 EP 2027264 A4 EP2027264 A4 EP 2027264A4 EP 07718958 A EP07718958 A EP 07718958A EP 07718958 A EP07718958 A EP 07718958A EP 2027264 A4 EP2027264 A4 EP 2027264A4
Authority
EP
European Patent Office
Prior art keywords
gene expression
expression vehicle
vehicle
gene
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP07718958A
Other languages
German (de)
English (en)
Other versions
EP2027264A2 (fr
EP2027264B1 (fr
Inventor
Marilyn Anne Anderson
Robyn Louise Heath
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hexima Ltd
Original Assignee
Hexima Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hexima Ltd filed Critical Hexima Ltd
Publication of EP2027264A2 publication Critical patent/EP2027264A2/fr
Publication of EP2027264A4 publication Critical patent/EP2027264A4/fr
Application granted granted Critical
Publication of EP2027264B1 publication Critical patent/EP2027264B1/fr
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Insects & Arthropods (AREA)
  • Pest Control & Pesticides (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
EP07718958.7A 2006-05-25 2007-05-24 Véhicule d'expression multigénique Not-in-force EP2027264B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80320606P 2006-05-25 2006-05-25
PCT/AU2007/000712 WO2007137329A2 (fr) 2006-05-25 2007-05-24 Véhicule d'expression multigénique

Publications (3)

Publication Number Publication Date
EP2027264A2 EP2027264A2 (fr) 2009-02-25
EP2027264A4 true EP2027264A4 (fr) 2010-02-03
EP2027264B1 EP2027264B1 (fr) 2014-10-29

Family

ID=38779010

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07718958.7A Not-in-force EP2027264B1 (fr) 2006-05-25 2007-05-24 Véhicule d'expression multigénique

Country Status (12)

Country Link
US (2) US20070277263A1 (fr)
EP (1) EP2027264B1 (fr)
JP (1) JP2009537155A (fr)
CN (1) CN101517077B (fr)
AR (1) AR063166A1 (fr)
AU (1) AU2007266307C1 (fr)
BR (1) BRPI0712168A2 (fr)
CA (1) CA2653404C (fr)
NZ (1) NZ572795A (fr)
SG (1) SG171689A1 (fr)
WO (1) WO2007137329A2 (fr)
ZA (1) ZA200809722B (fr)

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AU2002229407C1 (en) 2001-02-08 2005-09-01 Hexima Limited Plant-derived molecules and genetic sequences encoding same and uses therefor
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AR075257A1 (es) 2008-02-01 2011-03-23 Hexima Ltd Sistema de proteccion de plantas contra la infeccion por agentes patogenos
UY32035A (es) * 2008-08-05 2010-01-29 Hexima Ltd Sistemas antipatogenos
US9889184B2 (en) 2008-08-05 2018-02-13 Hexima Limited Anti-pathogen systems
SG192063A1 (en) 2011-02-07 2013-08-30 Hexima Ltd Modified plant defensins useful as anti-pathogenic agents
WO2013078433A1 (fr) 2011-11-23 2013-05-30 University Of Hawaii Domaines d'auto-traitement pour l'expression de polypeptidiques
US9567381B2 (en) 2012-03-09 2017-02-14 Vestaron Corporation Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
JP6457945B2 (ja) * 2012-11-23 2019-01-23 ヘキシマ リミテッドＨｅｘｉｍａ Ｌｉｍｉｔｅｄ 抗病原体方法
CN103114102B (zh) * 2013-01-29 2014-12-03 华南农业大学 一种多基因载体组装方法与应用
CA2901316A1 (fr) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Polypeptides phi-4 et leurs procedes d'utilisation
WO2015023846A2 (fr) 2013-08-16 2015-02-19 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
ES2937045T3 (es) 2013-09-13 2023-03-23 Pioneer Hi Bred Int Proteínas insecticidas y métodos para su uso
WO2015120270A1 (fr) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
MX2016010187A (es) 2014-02-07 2017-07-11 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
BR112017007932A2 (pt) 2014-10-16 2018-01-23 Du Pont proteínas inseticidas e métodos para uso das mesmas
CN116333064A (zh) 2015-05-19 2023-06-27 先锋国际良种公司 杀昆虫蛋白及其使用方法
EP3943602A1 (fr) 2015-08-06 2022-01-26 Pioneer Hi-Bred International, Inc. Protéines insecticides d'origine végétale et leurs méthodes d'utilisation
US20180325119A1 (en) 2015-12-18 2018-11-15 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2017156457A1 (fr) * 2016-03-11 2017-09-14 Donald Danforth Plant Science Center Protéines de défensine multimères et procédés associés
EP3960863A1 (fr) 2016-05-04 2022-03-02 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
MX2018015906A (es) 2016-07-01 2019-04-04 Pioneer Hi Bred Int Proteinas insecticidas de plantas y metodos para sus usos.
KR20190085514A (ko) 2016-10-21 2019-07-18 베스타론 코포레이션 절단성 펩타이드 및 이를 포함하는 살충 및 살선충 단백질
EP3535285B1 (fr) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
CN110088123B (zh) 2016-12-14 2023-10-20 先锋国际良种公司 杀昆虫蛋白及其使用方法
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
MX2019013321A (es) 2017-05-11 2020-02-10 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
MX2021002290A (es) 2018-08-29 2021-04-28 Pioneer Hi Bred Int Proteinas insecticidas y metodos para su uso.
CN110157710B (zh) * 2019-07-11 2022-12-27 贵州大学 花烟草NaD1基因启动子及其用途
US20230235352A1 (en) 2020-07-14 2023-07-27 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use

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LIN LI ET AL: "Efficient linking and transfer of multiple genes by a multigene assembly and transformation vector system", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE, WASHINGTON, DC, US, vol. 100, no. 10, 13 May 2003 (2003-05-13), pages 5962 - 5967, XP002323544, ISSN: 0027-8424 *
MILLER E A ET AL: "Identification of a novel four-domain member of the proteinase inhibitor II family from the stigmas of Nicotiana alata.", PLANT MOLECULAR BIOLOGY JAN 2000, vol. 42, no. 2, January 2000 (2000-01-01), pages 329 - 333, XP002559907, ISSN: 0167-4412 *
PLUNKETT G ET AL: "Proteinase inhibitors I and II from leaves of wounded tomato plants: Purification and properties", ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, ACADEMIC PRESS, US, vol. 213, no. 2, 1 February 1982 (1982-02-01), pages 463 - 472, XP024758590, ISSN: 0003-9861, [retrieved on 19820201] *
SCANLON M J ET AL: "Structure of a putative ancestral protein encoded by a single sequence repeat from a multidomain proteinase inhibitor gene fromNicotiana alata", STRUCTURE, CURRENT BIOLOGY LTD., PHILADELPHIA, PA, US, vol. 7, no. 7, 15 July 1999 (1999-07-15), pages 793 - 802, XP004742001, ISSN: 0969-2126 *
See also references of WO2007137329A2 *
THOMMA B P H J ET AL: "Plant defensins", PLANTA, SPRINGER VERLAG, DE, vol. 216, no. 2, 1 December 2002 (2002-12-01), pages 193 - 202, XP002404682, ISSN: 0032-0935 *

Also Published As

Publication number Publication date
AU2007266307B2 (en) 2012-05-31
EP2027264A2 (fr) 2009-02-25
EP2027264B1 (fr) 2014-10-29
ZA200809722B (en) 2009-11-25
JP2009537155A (ja) 2009-10-29
AU2007266307C1 (en) 2012-10-25
CN101517077B (zh) 2014-04-16
WO2007137329A2 (fr) 2007-12-06
SG171689A1 (en) 2011-06-29
CN101517077A (zh) 2009-08-26
US20070277263A1 (en) 2007-11-29
AU2007266307A1 (en) 2007-12-06
CA2653404A1 (fr) 2007-12-06
BRPI0712168A2 (pt) 2012-03-13
CA2653404C (fr) 2015-06-30
AR063166A1 (es) 2008-12-30
US20140259231A1 (en) 2014-09-11
WO2007137329A3 (fr) 2009-06-18
NZ572795A (en) 2011-06-30
WO2007137329A9 (fr) 2009-01-22

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