EP1979052A2 - Mouth and tooth care and cleansing agent comprising enzymes - Google Patents

Abstract

The invention relates to mouth and tooth care and cleansing preparations containing a mixture of an α-amylase and a mutanase as enzymes.

Description

 "Oral and Dental Care and Remedies with Enzymes"

The invention relates to preparations for oral and dental care and cleaning, which contain as enzymes a mixture of an α-amylase and a mutanase

Dentifrices are available in various forms on the market and serve primarily the cleaning of the tooth surface and the prevention of dental and gum disease They usually contain a combination of polishes, humectants, surfactants, binders, flavorings and fluoride-containing and antimicrobial agents In addition to toothpowder due to their increased abrasiveness play a minor role, dentifrices are offered mainly in paste, cream and translucent or transparent gel form In recent years, liquid or Flussigzahncremes and mouthwash have gained in importance

In addition to the cleaning of the teeth, the consumer expects from the generic products also a care of the teeth and the oral cavity Thus, in particular a "clean" feeling, ie a smooth and shiny tooth surface and a fresh feeling in the mouth essential aspects for the incentive to buy preparations to the mouth - and dental care and cleaning A successful means of the generic type should therefore thoroughly clean the teeth, without damaging the tooth or the tooth surface and at the same time reduce bad breath and / or prevent

In inaccessible areas of the mouth, such as in interdental spaces or the area of the posterior molars, the conventional mechanical cleaning with the toothbrush is often not completely successful, especially if the brushing time is insufficient. This has the consequence that the plaque in these areas is not complete is removed and microorganisms can settle back on the teeth, which leads to plaque formation

It has been known for a long time to use enzymes to solve these problems, wherein the enzymes deliberately degrade the constituents of the plaque and remove the plaque, but do not damage the teeth and the gums. In particular, the use of α-amylases and Mutanases have been described

Thus, toothpastes containing a protease and an amylase from Bacillus subtihs are described in US1232627

In DE1944308 dental and oral care products are described which contain enzyme complexes and / or enzyme mixtures of dextranases, other carbohydrases and / or proteases are called as carbohydrases alpha and / or beta-amylases and glucoamylases DE2000820 describes an oral care preparation containing a neutral protease and possibly small amounts of amylase or alkaline protease. It can be seen from the examples that the neutral protease was isolated from a Bacillus subtilis strain

In DE2408997 a mouth and tooth cleaning agent is described which contains a fructan-degrading enzyme and optionally additionally dextranase, amylase, protease or lipase

US4335101 claims a dental care composition containing dextranase and an enzyme for cleaving streptococcal-produced dental plaque glucan with mostly alpha-1,3-glucosillar linkage

WO95 / 31533 describes the use of a dextranase or 1, 3-glucanase in oral care agents. A source of the 1,3-glucanase is not mentioned

According to the invention it has now surprisingly been found that oral and dental care and - regimens containing a mixture of enzymes comprising an α-amylase and a mutanase have very particularly advantageous cleaning properties

Thus, a first subject of the present invention is oral and dental care and cleaning compositions containing a mixture of a mutanase and an α-amylase and / or an enzyme having a sequence homology or identity of at least 80%, preferably at least 90% preferably at least 95%, to the sequence according to Swiss-Prot P00691

In a preferred embodiment, the weight percent ratio of α-amylase to mutanase is from 6 to 1, in particular from 5 to 3, more preferably from 4 to 2, more preferably about 3 to 1

In a further preferred embodiment, the activity ratio of α-amylase to mutanase is from 90 l to 15 l, in particular from 75 l to 45 l, particularly preferably from 60 l to 30 l, above all it is approximately 45 l, based on the activity units (U) of the enzymes

In a preferred embodiment of the invention, the agent according to the invention contains no further enzyme in addition to the α-amylase and the mutanase

Optionally, however, in addition to the α-amylase and the mutanase in the inventive means also other enzymes may be contained These may in particular be selected from proteases, amylases and hemicellulases In a further preferred embodiment, the additional enzyme optionally contained is selected from the group consisting of β-glucanase, in particular from Bacillus, preferably from Bacillus amyloliquefaciens, and / or an enzyme having a sequence homology or identity of at least 80%, preferably at least 90% , particularly preferably at least 95%, to the sequence according to Swiss-Prot Q8GMY0, protease B7020, in particular from Aspergillus, preferably from Aspergillus oryzae, neutral protease, in particular from Aspergillus, preferably from Aspergillus oryzeae, and Corolase, in particular from Aspergillus, preferably from Apergillus sojae

Oral and dental care as well as oral and dental agents in the context of the invention are especially oral and dental powders, oral and toothpastes, liquid mouth and tooth creams, oral and dental gels, mouthwash and chewing gums and toothpaste and cleaning agents z B in the form of toothpastes, liquid toothpastes, toothpowder mouthwashes or possibly also as chewing gum, eg as chewing gum, are present. However, they are preferably present as more or less flowable or plastic toothpastes, as they are for cleaning the teeth Under use of a toothbrush are under oral and Zahnpflege- and -reinigungsmittel according to the invention further, in a less preferred embodiment, also denture care products to understand, in particular denture cleanser and denture adhesive creams

The cc-amylase suitable according to the invention can be derived from any organism. In a preferred embodiment of the invention, the cc-amylase is a bacterial enzyme, in particular an enzyme from Bacillus, particularly preferably from Bacillus subtihs The B-subtilis α-amylase In particular, the α-amylase may be an enzyme having a sequence according to Swiss-Prot P00691, EMBL (DNA) Z99105 or an enzyme having a sequence identity or homology to this sequence of at least 80%, preferably at least 90%, particularly preferably at least 95% act

The mutanase which is suitable according to the invention may in particular be a mutanase from a known dextranase-producing bacterium, in particular a mutanase from Pseudomonas, Tπchoderma, Streptomyces, Aspergillus, Flavobacteπum or Bacillus. Another name for the mutanase is alpha-1, 3 Glucanase According to the IUBMB nomenclature, the mutanase is designated EC 3 2 1 59

The β-glucanase from B amyloliquefaciens suitable according to the invention is designated in the IUBMB enzyme nomenclature with the number EC 3 2 1 6, the recommended name for these enzymes is 1,3 (4) -b-glucanase. Other names are endo-1, 3-bD-glucanase, laminaπnase, laminaranase, b-1 3-glucanase, b-1, 3-1, 4-glucanase, endo-1,3-b-glucanase, endo-b-1,3 (4) - Glucanase, endo-b-1,3-1,4-glucanase, endo-b- (1 → 3) -D-glucanase, endo-1, 3-1, 4-bD-glucanase, endo-b- 1-3) -D Glucanase, endo-b-1, 3-glucanase IV or endo-1 3-bD-glucanase The systematic name is 1,3- (1,3,1,4) -bD-glucan 3 (4) -glucanohydrolase it is in the ß-glucanase an enzyme with a sequence according to Swiss-Prot Q8GMY0, EMBL AAN64132 EMBL (DNA) AF546871

The invention suitable neutral protease from Aspergillus oryzae is referred to in the IUBMB Enzyme Nomenclature with the number EC 3 4 24 28

The corolase from Aspergillus sojae suitable according to the invention is designated in the IUBM B nomenclature by the numbers EC 3 4 21 15 and 3 4 21 63

The enzyme activities of the enzymes used in the oral and dental care and cleaning agents according to the invention may vary from enzyme to enzyme. Usually the enzyme activity per gram of the oral and dental care and cleaning agent according to the invention is between 0.1 and 1500 U (μmol / min).

Oral and dental care and cleaning preparations according to the invention are preferably characterized in that the total enzyme activity per gram oral and dental care and cleaning agent is from 0.05 to 1500 U, preferably from 0 1 to 1200 U _1, particularly preferably from 0, 25 to 1000 U and in particular from 0.5 to 500 U amounts

Particularly preferred

values for the alpha-amylase of from 10 to 1500 U, preferably from 30 to 700 U, more preferably from 50 to 500 U and in particular from 75 to 250 U per gram of the oral and dental care and cleaning agent according to the invention,

values for the mutanase of from 0.1 to 100 U, preferably from 0.2 to 50 U and in particular from 0.5 to 20 U per gram of the oral and dental care and cleaning agent according to the invention,

values for the beta-glucanase of from 0.1 to 100 U, preferably from 0.25 to 50 U, more preferably from 0.5 to 25 U and in particular from 0.5 to 5 U per gram of the oral and dental care of the invention and cleaning agent,

for the proteases, values of 0.1 to 100 U, preferably of 0.25 to 50 U, more preferably of 0.5 to 25 U and in particular of 0.5 to 5 U per gram of the oral and Zahnpflege- and -reinigungsmittels

for the corolase, values of 0.1 to 100 U, preferably of 0.25 to 50 U, more preferably of 0.5 to 25 U and in particular of 0.5 to 5 U per gram of the oral and dental care and -reinigungsmittels

The enzyme activities are measured in the context of the present invention at 25 ° C, pH = 7.0 in a 100 mM potassium phosphate buffer, for the activity determination of proteases and the corolase finds the AAPF method, for the activity determination of alpha-amylase and the Mutanase the DNA method and for the activity determination of the beta-glucanase the PAHBAH method application

The oral and dental care and cleaning compositions according to the invention may additionally contain other ingredients of oral cleansing preparations, oral hygiene products, dentifrices and / or dentifrices. The preferred further ingredients are described below.

The oral and dental care and cleaning compositions according to the invention preferably contain water as a further essential ingredient. The water contained in the oral and dental care and cleaning compositions according to the invention may be tap water, the degree of hardness of which may vary depending on the place of manufacture or source of the water. However, it is possible and preferred to use water with degrees of hardness between 0 and 20 ° dH, preferably between 1 and 16 ° dH. Particularly preferred is the use of technically demineralized water ("water VE"), which has been largely freed from salts with the aid of ion exchangers.

As humectants, the agents according to the invention may e.g. Glycerol, sorbitol, xylitol, propylene glycols, polyethylene glycols or mixtures of these polyols, especially those polyethylene glycols having molecular weights of 200-2000 g / mol, preferably from 200 to 1000 g / mol

The compositions according to the invention, in particular toothpastes or liquid toothpastes, in one preferred embodiment contain one or more polishing agents, usually in a total amount of from 5 to 50% by weight.

In principle, all known for toothpastes Reibkorper, especially those that do not contain calcium ions are suitable as a polishing agent. Preferably suitable polishing agent components are therefore silicic acids, aluminum hydroxide, aluminum oxide, sodium aluminum silicates, organic polymers or mixtures of such Reibkorper.

Calcium containing polishing components such as e.g. However, chalk, calcium pyrophosphate, dicalcium phosphate dihydrate can be present in amounts of up to 5% by weight

Toothpastes and liquid dentifrices containing silicic acids as polishing agents are particularly preferred. Suitable silicic acids are, for example, gel silicas, hydrogel silicic acids and precipitated silicas. Gel silicas are prepared by reacting sodium silicate solutions with strong, aqueous mineral acids to form a hydrosol, aging to a hydrogel, washing and drying. If drying takes place under mild conditions to a water content of 15 to 35% by weight, the so-called hydrogel silicic acids are obtained Drying to water contents below 15% by weight results in an irreversible shrinkage of the previously loose structure of the hydrogel to the dense structure of the so-called xerogel

A second, preferably suitable group of silicic acid pouchers are the precipitated silicas. These are obtained by precipitation of silica from dilute alkali silicate solutions by addition of strong acids under conditions in which aggregation to the sol and gel can not occur. Suitable methods are preferred a precipitated silica having a BET surface area of 15-110 m 2 / g, a particle size of 0.5-20 μm, at least 80% by weight of the particles being below 5 μm, and a viscosity in 30% glycerol-water. (1 1) -Dιspersιon of 30 - 60 Pa s (20 ° C) in an amount of 10 - 20% by weight of the toothpaste Preferably suitable cashews of this type also have rounded corners and edges and are under the trade name Sιdent12 DS (DEGUSSA ) available

Other cashew silicas of this type are Sident 8 (DEGUSSA) and Sorbosil AC 39 (Crosfield Chemicals). These silicas are characterized by a lower thickening effect and a somewhat higher average particle size of 8-14 μm with a specific surface area of 40-75 m 2 / g BET) and are particularly suitable for liquid toothpastes These should have a viscosity (25 ° C, shear rate D = 10 s-1) of 10 - 100 Pa s

On the other hand, toothpastes which have a significantly higher viscosity of more than 100 Pa s (25 ° C., D = 10 s-1) require a sufficiently high proportion of silicas having a particle size of less than 5 μm, preferably at least 3% by weight. Such a toothpaste is therefore preferably used in addition to the cited silica casts even finer, so-called thickened silica with a BET surface area of 150-250 m2 / g, for example the commercial products Sipernat 22 LS or Sipernat 320 DS

Aluminum oxide in the form of slightly calcined alumina containing alumina and aluminum oxide in an amount of about 1-5% by weight may also be present as further polishing agent component. Such suitable alumina is commercially available under the trade name 'Poliantonerde P10 feinst' (Giulini Chemistry) available

Other suitable poherizing agents are all friction bodies known for toothpastes, such as, for example, sodium aluminosilicates, such as, for example, zeolite A, organic polymers, such as polymethacrylate, or mixtures of these and the abovementioned friction bodies

In summary, oral and dental care and cleaning compositions according to the invention are preferred which additionally have cleaning bodies, preferably silicic acids, aluminum hydroxide, aluminum oxide, calcium pyrophosphate, chalk dicalcium phosphate dihydrate (CaHPO ₄ .2H ₂ O), sodium aluminum silicates, in particular zeolite A, organic polymers, in particular Polymethacrylates or mixtures of these friction bodies, preferably in amounts of from 1 to 30% by weight, particularly preferably from 2.5 to 25% by weight and in particular from 5 to 20% by weight, in each case based on the total agent.

The oral and dental care and cleansing agents according to the invention, in particular the toothpastes, can, for. B. also contain antimicrobial substances as preservatives or as antiplaque agents. Such substances may, for example, be selected from methyl p-hydroxybenzoate, ethyl or propyl ester, sodium sorbate, sodium benzoate, bromochlorophene, triclosan, phenyl salicylate, biguanides, e.g. B chlorhexidine and thymol. These substances are in the agents according to the invention preferably in amounts of 0.1 to 5 wt.%, Particularly preferably from 0.25 to 2.5 wt.% And in particular from 0.5 to 1, 5 wt.%, Each based on the entire remedy, included

The oral and dental care and cleaning agents, in particular the toothpastes, may also contain substances that are active against tartar. Such substances may be, for example, chelating agents such. As ethylenediaminetetraacetic acid and its sodium salts, pyrophosphate salts such as the water-soluble dialkali or Tetraalkalimetallpyrophosphat- salts, z. B. Na ₄ P ₂ O ₇ , K ₄ P ₂ O ₇ , Na ₂ K ₂ P ₂ O ₇ , Na ₂ H ₂ P ₂ O ₇ and K ₂ H ₂ P ₂ O ₇ or polyphosphate salts, the z. B. from water-soluble Alkalimethalltripolyphosphaten Natriumtnpolyphosphat and Kaliumtπpolyphosphat may be selected. According to the invention, preferred oral and dental care and cleaning agents are characterized in that they additionally phosphate (s), preferably Alkalιmetallphosphat (s) and in particular Natπumtripolyphosphat, preferably in amounts of 1 to 10 wt .-%, particularly preferably from 2 to 8 wt .-% and in particular from 3 to 7 wt .-%, each based on the total agent included.

Another preferred group of ingredients that may be included in the compositions of this invention are anticonvulsants. These may be selected, for example, from organic or inorganic fluorides, for. B. from Natriumfluoπd, potassium fluoride, sodium monofluorophosphate, sodium fluorosilicate, zinc fluoride and Zιnn- (ll) -fluorιd. Preferably, an amount of 0.01-0.5% by weight of fluorine should be included in the form of said compounds.

The compositions according to the invention, in particular the toothpastes, may additionally contain further wound-healing and anti-inflammatory substances, for example active substances for gingivitis. Such substances may be selected, for example, from allantoin, azulene, chamomile extracts, tocopherol, panthenol, bisabolol, sage extracts.

Suitable non-cationic, bactericidal components are, for example, phenols, resorcinols, bisphenols, salicylanilides and their halogenated derivatives, halogenated carbanides and p-hydroxybenzoic acid esters. Particularly preferred antimicrobial components are halogenated diphenyl ethers, for example 2,4-dichloro-2'-hydroxydiphenyl ether, 4 4'-Dιchlor-2'-hydroxydιphenylether, 2,4,4'-Tπbrom-2'-hydroxydphenyl ethers and 2,4,4'-trichloro-2'-hydroxydiphenyl ethers (triclosan) They are preferably used in amounts of 0.01 to 1 wt .-% in the inventive dentifrice , Triclosan is particularly preferably used in an amount of 0.01-0.3% by weight.

D-panthenol D - (+) - 2,4-Dihydroxy-N- (3-hydroxypropyl) -3,3-dimethyl-butyramide shows a biological activity corresponding to pantothenic acid. Pantothenic acid (R - (+) - N - (2 , 4-Dihydroxy-3,3-dimethylbutyryl-β-alanine) is a precursor in the biosynthesis of coenzyme A and is counted to the vitamin B complex (B3) These substances are known to promote wound healing and a They have therefore also occasionally been described in toothpastes. The toothpastes according to the invention preferably contain 0.05-5% by weight of panthenol or a salt of pantothenic acid

Retinol (3,7-dimethyl-9- (2,6,6-tri-methyl-1-cyclohexenyl) -2,4,6,8-nonatetraen-1-ol is the international common name for vitamin Al instead of retinol may also one its derivatives having a similar biological action, for example an ester or the retinoic acid (tretinoin), one of its salts or its esters are preferably used A retinol ester, in particular a fatty acid ester of a fatty acid having 12-22 carbon atoms is used When using a retinol ester, for example retinol palmitate with an activity of 1.7 10 ⁵ 1 U per g, an amount of 0.001 to 0.1% by weight is preferred when using other retinol derivatives an amount corresponding to a concentration of 10 ³ to 10 ⁶ IE (International Units) per 100 g

Preferred dentifrices according to the present invention preferably contain, in addition to polishing agents, fluorine compounds, humectants and binders

0.01 to 1% by weight of a halogenated diphenyl ether

0.05 - 5 wt .-% panthenol or a salt of pantothenic acid and

0.01-0.1% by weight of a retinol ester, preferably retinol palmitate

The binders or consistency regulators used are, for example, natural and / or synthetic water-soluble polymers such as alginates, carrageenates, tragacanths, starch and starch ethers, cellulose ethers such as carboxymethylcellulose (Na-SaIz), hydroxyethylcellulose, methylhydroxypropylcellulose, guar, acacia, agar, xanthan Gum, succinoglycan gum, locust bean flour, pectins, water-soluble carboxyvinyl polymers (eg Carbopol® types), polyvinyl alcohol, polyvinylpyrrolidone, polyethylene glycols, in particular those with molecular weights of 1 500-1 000 000

Other substances that are suitable for viscosity control, z. B. phyllosilicates such as montmorillonite clays, colloidal thickened silicas such. As airgel silicas, fumed silicas or finely ground precipitated silicas. It is also possible viscosity-stabilizing additives from the group of cationic, zwitterionic or ampholytic nitrogen-containing surfactants, the hydroxypropyl-substituted hydrocolloids or the Polyethylene glycol / polypropylene glycol copolymers having an average molecular weight of 1000 to 5000 or a combination of said compounds may be used in the toothpastes

Surface-active substances may also be present in the toothpastes in support of the cleaning action and, if desired, also for the development of foam during toothbrushing and for stabilizing the body dispersion in the carrier preferably in an amount of 0.1-5% by weight

In particular, alkyl- and / or alkenyl- (oligo) -glycosides can be used as surfactants. Their preparation and use as surface-active substances are described, for example, in US Pat. No. 3,839,318, US Pat. No. 3,707,535, US Pat. No. 3,547,307 828 DE-A-1943 689, DE-A-20 36 472 and DE-A-30 01 064 and EP-A-77 167. With regard to the glycoside radical, it is true that both monoglycosides (x = 1) in which a pentose or Hexoserest glycosidically bound to a primary alcohol having 4 to 16 carbon atoms, as well as oligomeric glycosides having a Oligomeπsationsgrad x to 10 are suitable The Oligomeπsationsgrad is a statistical mean, which is based on a common for such technical products homolog distribution

Preferred alkyl and / or alkenyl (oligo) glycosides are alkyl and / or alkenyl (oligo) glucosils of the formula RO (C ₆ H ₁₀ O) _x -H, in which R is an alkyl radical and / or alkenyl group having 8 to 14 carbon atoms and x is an average value of 1 to 4. Especially preferred are Alkylohgo- glucoside based on hydrogenated _{C12 /} i ₄ coconut alcohol with a DP of 1 to 3, the alkyl and Alkenyl glycoside surfactant can be used very sparingly, even amounts of 0.005 to 1% by weight being sufficient

In addition to the alkyl glucoside surfactants mentioned, other nonionic, ampholytic and cationic surfactants may also be present, such as fatty alcohol polyglycol ethersulfates, monoglycerides, monoglycerides, mono- and / or dialkyl sulfosuccinates, fatty acid isethionates, fatty acid sarcosinates, fatty acid units, fatty acid glutamates, ether carboxylic acids, fatty acid glucamides, alkylamido -betaines and / or protein fatty acid condensates, the latter preferably based on wheat proteins A mordionogenic solubilizer from the group of surface-active compounds may be required in particular for solubilizing the most water-insoluble aromatic moieties. or sorbitan-oxyethylates Solubilizers from the group of oxethylated fatty acid glycerides mainly comprise addition products of 20 to 60 moles of ethylene oxide on mono- and diglycidene of linear fatty acids having 12 to 18 carbon atoms or on triglycerides of Hydroxyfettsauren such as Oxysteaπnsäure or ricinic acid Further suitable solubilizers are ethoxylated Fettsäuresorbitanpartialester, which are preferably addition products of 20 to 60 moles of ethylene oxide to sorbitan monoesters and sorbitan of fatty acids having 12 to 18 carbon atoms Also suitable solubilizers are fatty acid partial esters of glycine or sorbitan oxyethylates, these are preferably mono- and Diesters of C ₁₂ -C ₈ fatty acids and adducts of 20 to 60 moles of ethylene oxide with 1 mole Glyceπn or 1 mole of sorbitol.

The oral, dental and / or dental prosthesis care agents according to the invention preferably contain, as solubilizers for optionally contained aroma oils, adducts of 20 to 60 moles of ethylene oxide with hardened or unhardened castor oil (ie with oxystearic acid or ricinoleic acid), with glycine mono- and or distearate or sorbitan mono- and / or distearate

Suitable surfactants for inventive compositions are further z. B. Linear Natπumalkylsulfate with 12-18 C-atoms in the alkyl group These substances also have an enzyme-inhibiting effect on the bacterial metabolism of plaque. Further suitable surfactants are Alkahsalze, preferably sodium salts of Alkylpolyglycolethersulfat with 12-16 C atoms in the linear alkyl group and 2-6 glycol ether groups in the molecule, of linear alkane (C ₂ - C ₁₈₎ - sulfonate, esters of SulfobernsteinsäuremonoalkyKC ^ -C of sulfated Fettsäuremonoglyceπdeπ, sulfated fatty acid alkanolamides, Sulfoessιgsäurealkyl (C ₂ -C ₆₎ - esters, acyl sarcosines, acyl taurides and Acyhsothionaten each having 8-18 carbon atoms in the acyl group. Zwitterionic, ampholytic and nonionic surfactants are also suitable, for example oxethylates of fatty acid mono- and diglycerides, of fatty acid sorbitan esters and alkyl (oligo) glucosides.

The oral and dental care products, in particular the toothpastes, may also contain the insensitivity of the teeth-enhancing substances, for example potassium salts such as potassium nitrate, potassium citrate, potassium chloride, potassium bicarbonate and potassium oxalate. These substances are preferably present in the compositions according to the invention in amounts of from 0.5 to 20 % By weight, more preferably from 1.0 to 15% by weight, more preferably from 2.5 to 10% by weight and in particular from 4.0 to 8.0% by weight, in each case based on the total agent.

The compositions according to the invention, in particular the toothpastes, may also contain substances for increasing the mineralizing potential, for example calcium-containing substances such as calcium chloride, calcium acetate and dicalcium phosphate dihydrate. The concentration of the calcium-containing substance depends on the solubility of the substance and the interaction with other substances contained in the oral and dental care products. A particularly effective remineralizing substance is the calcium glycerophosphate, the calcium salt of Glyceπn-1-phosphoric acid or glycerol-2-phosphoric acid or the glycerol-1-phosphoric acid enantiomeric Glycerιn-3-phosphoric acid - or a mixture of these acids The Compound has a remineralizing effect in dentifrices as it provides both calcium and phosphate ions. In the dentifrices according to the invention, calcium glycerophosphate is preferably used in amounts of 0.01-1% by weight. The dentifrice and / or oral hygiene products according to the invention can be improved by adding aroma oils and sweeteners in their organoleptic properties. As aroma oils, all natural and synthetic flavors customary for oral, dental and / or dental prosthesis care agents are suitable. Natural flavors can be used both in the form of the essential oils isolated from the drugs and the individual components isolated from them. Preferably, at least one aromatic oil from the group of peppermint, spearmint oil, aniseed oil, caraway oil, eucalyptus oil, fennelol, cinnamon, geranium oil, sage oil, thyme oil, marjoram oil, basil oil, citrus oil, Gaultheria oil or one or more synthetic components of these oils isolated therefrom should be included. The most important components of the oils mentioned are, for example, menthol, carvone, anethole, cineole, eugenol, cinnamaldehyde, geraniol, citronellol, linalool, salvos, thymol, terpinene, terpinol, methylchavicol and methyl salicylate. Further suitable flavors are, for example, menthyl acetate, vanillin, ionone, linalyl acetate, rhodinol and piperone. Suitable sweeteners are either natural sugars such as sucrose, maltose, lactose and fructose or synthetic sweeteners such as saccharin sodium salt, sodium cyclamate or aspartame.

Further customary auxiliaries and additives for toothpastes are

Solvents and solubilizers, for example lower monohydric or polyhydric alcohols or ethers, e.g. Ethanol, 1, 2-propylene glycol, diethylene glycol or butyl diglycol

Pigments, such as titanium dioxide

Dyes pH adjusting agents and buffer substances, such as, for example, sodium bicarbonate, sodium citrate,

Sodium benzoate, citric acid, phosphoric acid or acid salts, eg NaH ₂ PO ₄ , other wound-healing or anti-inflammatory substances, eg allantoin, urea,

Azulene, chamomile agents, acetylsalicylic acid derivatives or rhodanide other vitamins such as e.g. Ascorbic acid, biotin, tocopherol! or rutin

Mineral salts such as manganese, zinc or magnesium salts

Another important group of ingredients that may be included in the compositions of the invention are the so-called bioactive glasses.

In the context of the present application, the term "bioactive glasses" encompasses glasses which are biologically active and / or biologically active The biological effectiveness of a glass can be demonstrated in its antimicrobial properties, for example, biologically active glass differs from conventional lime-sodium silicate Biologically active glass, for example, refers to a glass which forms a firm bond with body tissue, forming a hydroxyl apatite layer. Bioactive glass is also understood as meaning a glass which has antimicrobial and / or anti-inflammatory action The glass powders have a biocidal or biostatic effect against bacteria, fungi and viruses, are tolerated by the skin in contact with humans, are toxicologically harmless and, in particular, are also suitable for consumption Particularly preferred oral and dental care and cleaning compositions according to the invention are characterized in that they contain from 0.2 to 20% by weight, preferably from 0.4 to 14% by weight, particularly preferably from 0.5 to 3% by weight and in particular 0, 6 to 2% by weight of at least one bioactive glass

The oral and dental care and cleaning compositions according to the invention of this embodiment contain bioactive glass or glass powder or glass ceramic powder or composite materials which comprise such a bioactive glass. Within the scope of the present application, glass powders are also understood as meaning granules and glass beads

Due to the requirements for the toxicological safety of the glass and its suitability for consumption, the glass powder should be particularly pure. The heavy metal exposure is preferably low. Thus, the maximum concentration in the range of cosmetic formulations is preferably Pb <20 ppm, Cd <5 ppm, As < 5 ppm, Sb <10 ppm, Hg <1 ppm, Ni <10 ppm

The unkeramisierte starting glass, which is contained directly in the preferred compositions according to the invention or optionally used for the production of a glass ceramic useful in the invention, contains SiO ₂ as a network former, preferably between 35-80 wt -% At lower concentrations, the spontaneous Kristalhsationsneigung greatly increased and the chemical Stability severely degrading At higher SιO ₂ values, the Kπstallisationsstabilität may decrease, and the processing temperature is significantly increased, so that the hot forming properties deteriorate Na ₂ O is used as a flux in melting the glass At concentrations less than 5%, the melting behavior is negatively influenced sodium Part of the phases which form during the ceramization and, if high crystalline phase proportions are to be set by the ceramization, must be present in correspondingly high concentrations in the glass. K ₂ O acts as a flux In addition, potassium is released in aqueous systems. If high potassium concentrations are present in the glass, potassium-containing phases such as calcium silicates are also eliminated. The chemical stability of the glass and glass can be determined by the P ₂ O ₅ content of sihkatischen glasses, glass ceramics or composites In order for the ion donation to be adjusted in aqueous media, P ₂ O ₅ network images are preferred. The P ₂ O content is preferably between 0 and 80% by weight. To improve the meltability, the glass can be up to 25% by weight B ₂ O ₃ contain Al ₂ O ₃ is used to adjust the chemical resistance of the glass

To enhance the antimicrobial, in particular the antibacterial properties of the glass ceramic, antimicrobial ions such as Ag, Au, I, Ce, Cu, Zn can be present in concentrations of less than 5% by weight Coloring ions such. As Mn, Cu, Fe, Cr, Co, V, may be contained individually or in combination, preferably in a total concentration less than 1 wt .-%.

Usually, the glass or the glass ceramic is used in powder form. The ceramization can be done either with a glass block or Glasnbbons or with glass powder. After ceramization, the glass-ceramic blocks or ribbons must be ground to powder. If the powder has been ceramified, it may also be necessary to re-mill to remove agglomerates formed during the ceramization step. The milling operations can be carried out both dry and in aqueous or non-aqueous milling media. Usually, the particle sizes are less than 500 μm. As appropriate, particle sizes <100 microns or <20 microns have been found. Particularly suitable are particle sizes <10 microns and less than 5 microns and less than 2 microns, see below.

The bioactive glasses or glass powder or glass ceramic powder or composite compositions contained in the preferred compositions according to the invention comprise glasses which preferably comprise the following components: SiO ₂ 35-80% by weight, Na ₂ O '0-35% by weight, P ₂ O ₅ . 0-80 wt%, MgO. 0-5 wt.%, Ag ₂ O: 0-0.5 wt.%, AgJ: 0-0.5 wt.%, NaJ 0-5 wt.%, TiO ₂ 0-5 wt. %, K ₂ O: 0-35 wt.%, ZnO: 0-10 wt.%, Al ₂ O ₃ . 0-25% by weight and B ₂ O ₃ : 0-25% by weight

Furthermore, the base glass according to the above composition to achieve further effects such as color or UV filtering ions such as Fe, Co, Cr, V, Ce, Cu, Mn, Ni, Bi, Sn, Ag, Au, J individually or in total to be added to 10% by weight. A further glass composition may be as follows: SiO ₂ : 35-80 wt%, Na ₂ O: 0-35 wt%, P ₂ O ₅ 0-80 wt%, MgO. 0-5 wt.%, Ag ₂ O 0-0.5 wt.%, AgJ: 0-0.5 wt.%, NaJ 0-5 wt.%, TiO ₂ : 0-5 wt. %, K ₂ O: 0-35% by weight, ZnO 0-10% by weight, Al ₂ O ₃ : 0-25% by weight, B ₂ O ₃ - 0- 25% by weight, SnO: 0-5 wt .-%, CeO ₂ - 0-3 wt -% and Au 0.001 - 0.1 wt .-%.

Particularly preferred oral and dental care and cleaning agents according to the invention are characterized in that the bioactive glass - based on its weight - has the following composition:

SiO ₂ 35 to 60% by weight, preferably 40 to 60% by weight

Na ₂ O 0 to 35% by weight, preferably 5 to 30% by weight,

K ₂ O 0 to 35 wt .-%, preferably 0 to 20 wt .-%,

P ₂ O ₅ O to 10 wt .-%, preferably 2 to 10 wt .-%,

MgO 0 to 10% by weight, preferably 0 to 5% by weight,

CaO 0 to 35% by weight, preferably 5 to 30% by weight,

Al ₂ O ₃ 0 to 25 wt .-%, preferably 0 to 5 wt -%,

B ₂ O ₃ 0 to 25% by weight, preferably 0 to 5% by weight,

TiO ₂ O to 10 wt.%, Preferably 0.1 to 5 wt.% As already mentioned above, the bioactive glass is preferably used in particulate form. Here are particularly preferred oral and dental care and - cleaning agent according to the invention characterized in that the antimicrobial glass particle sizes <10 .mu.m, preferably from 0.5 to 4 .mu.m, more preferably from 1 to 2 microns.

It has been found that the enzyme-containing oral and dental care and cleaning agents according to the invention can be further increased in their performance if the agents contain salivationsforderndeende substances

Salivation is the production and release of saliva, in a broader sense also in an unphysiologically increased amount. Substances that stimulate salivary flow and increase the amount and / or release of saliva can come from a wide variety of substance classes

An example according to the invention suitable substance is pilocarpine, which may be included in the oral and dental care and cleaning agents according to the invention

Pilocarpi

Other saliva-demanding substances are in particular so-called pungent substances, that is, pungent-tasting and / or a feeling of heat-producing substances. Oral and dental care and cleaning agents which are preferred according to the invention are characterized in that they contain at least one pungent-tasting and / or heat-generating substance as salivation-requiring substance.

As a salivation-promoting ingredient, the inventive products of this embodiment contain a pungent-tasting and / or a feeling-of-heat-generating substance. These substances give the user a sharp, tingling, mouth-watering or heat-generating effect, i. they cause a sensation of warmth or burning, or tingling, beads, tickling or bubbling, thereby promoting salivation

Products of this embodiment which are preferred according to the invention contain the pungent-tasting and / or a feeling of heat-generating substance (s) in amounts of from 0.00001 to 5% by weight, preferably from 0.0005 to 2.5% by weight .-%, more preferably from 0.001 to 1 wt .-%, particularly preferably from 0.005 to 0.75 wt .-% and in particular from 0.01 to 0.5% by weight, each based on the weight of the total composition As a pungent-tasting or a feeling of heat-generating substance, a number of substances are used are preferred in particular N-alkyl-substituents amides of unsaturated carboxylic acids, for example

2E, 4E-Decadιensaure-N-Methylamιd

2E, 4E-Decadιensäure-N-Ethylamιd

2E, 4E-Decadιensäure-N-n-Propylamιd

2E, 4E-Decadιensaure-N-lsopropylamιd

2E, 4E-Decadιensäure-N-n-Butylamιd

2E, 4E-Decadιensaure-N- (1-methylpropyl) -amιd

2E, 4E-Decadιensaure-N-lsobutylamιd

2E, 4E-Decadιensäure-N-tert-Butylamιd

2E, 4Z-Decadιensaure-N-Methylamιd

2E, 4Z-Decadιensaure-N-Ethylamιd

2E, 4Z-Decadιensaure-N-n-Propylamιd

2E, 4Z-Decadιensaure-N-lsopropylamιd

2E, 4Z-Decadιensäure-N-n-Butylamιd

2E, 4Z-decadienoic acid-N- (1-methylpropyl) -amide

2E, 4Z-Decadιensaure-N-lsobutylamιd

2E, 4Z-Decadιensäure-N-tert-Butylamιd

2E, 4E, 8Z-Decatrιensaure-N-Methylamιd

2E, 4E, 8Z-Decatrιensäure-N-Ethylamιd

2E, 4E, 8Z-Decatrιensäure-N-n-Propylamιd

2E, 4E, 8Z-Decatrιensäure-N-lsopropylamιd

2E, 4E, 8Z-Decatrιensäure-N-n-Butylamιd

2E, 4E, 8Z-Decatrιensäure-N- (1-methylpropyl) -amιd

2E, 4E, 8Z-Decatrιensäure-N-lsobutylamιd

2E, 4E, 8Z-Decatrιensäure-N-tert-Butylamιd

2E, 4Z, 8Z-Decatrιensäure-N-lvlethylamιd

2E, 4Z, 8Z-Decatrιensäure-N-Ethylamιd

2E, 4Z, 8Z-Decatrιensäure-N-n-Propylamιd

2E, 4Z, 8Z-Decatrιensäure-N-lsopropylamιd

2E, 4Z, 8Z-Decatrιensäure-N-n-Butylamιd

2E, 4Z, 8Z-Decatrιensäure-N- (1-methylpropyl) -amιd

2E, 4Z, 8Z-Decatrιensäure-N-lsobutylamιd

2E, 4Z, 8Z-Decatrιensäure-N-tert-Butylamιd E, 4E, 8E-decatrienoic acid N-methylamide E, 4E, 8E-decatrienoic acid N-ethylamide E, 4E, 8E-D8catrioenoic acid-Nn-propylaniline E, 4E, 8E-decatric acid N-isopropylamide E, 4E, 8E- Decatrienoic acid-Nn-Butylamιd E, 4E, 8E-Decatriensaure-N- (1-methylpropyl) -amide E, 4E, 8E-Decatriensäure-N-isobutylamide E, 4E, 8E-DecatrÃensrenic acid N-tert-butylamιd

E, 4Z, 8E-decatrienoic acid N-methylamide E, 4Z, 8E-decatrienoic acid N-ethylamide E, 4Z, 8E-decatrienoic acid Nn-propylamide E, 4Z, 8E-decatonic acid N-isopropylamide E, 4Z, 8E- Decatrienoic acid-Nn-Butylamιd E, 4Z, 8E-Decatriensäure-N- (1-methylpropyl) -amιd E, 4Z, 8E-Decatriensäure-N-isobutylamide E, 4Z, 8E-Decatriensäure-N-tert-butylamιd

E, 6Z, 8E-decatrienoic acid N-methylamide E, 6Z, 8E-decatrienoic acid N-ethylamine E, 6Z, 8E-decatrienoic acid Nn-propylamide E, 6Z, 8E-decatrienoic acid N-isopropylamide E, 6Z, 8E Decatriensaure-Nn-Butylamιd E, 6Z, 8E-Decatriensäure-N- (1-methylpropyl) -amide E, 6Z, 8E-Decatriensäure-N-lsobutylamιd E, 6Z, 8E-Decatriensäure-N-tert-butylamιd

E, 6E, 8E-decatrienoic acid N-methylamide E, 6E, 8E-decatrienoic acid N-ethylamide E, 6E, 8E-decatrienoic acid Nn-propylamide E, 6E, 8E-decatrienoic acid N-isopropylamide E, 6E, 8E- Decatriensaure-Nn-butylamide E, 6E, 8E-Decatrieπsaure-N- (1-methylpropyl) -amιd E, 6E, 8E-Decatriensäure-N-lsobutylamιd E, 6E, 8E-Decatπensäure-N-tert-butylamide

E, 6Z, 8Z-decatrienoic acid-N-methylamide E, 6Z, 8Z-decatric acid N-ethylamide E, 6Z, 8Z-decatrienoic acid-Nn-propylamide E, 6Z, 8Z-decatrienoic acid-N-isopropylamide 2E, 6Z, 8Z-Decatriensäure-Nn-Butylannicl

2E, 6Z, 8Z-Decatriensäure-N- (1-methylpropyl) -amide

2E, 6Z, 8Z-Decatriensaure-N-lsobutylamid

- 2E, 6Z, 8Z-Decatriensäure-N-teιi-Butylamιd

2E, 6E, 8Z-decatric acid N-methylamide 2E, 6E, 8Z-decatrienoic acid N-ethylamide 2E, 6E, 8Z-decatrienoic acid Nn-propylamide 2E, 6E, 8Z-decatrienoic acid N-isopropylamide 2E, 6E, 8Z -Decatrolenic acid-Nn-Butylamιd 2E, 6E, 8Z-Decatrieπsäure-N- (1-methylpropyl) -amide

2E, 6E, 8Z-decatrienoic acid N-isobutylamide

- 2E, 6E, 8Z-Decatriensaure-N-tert-butylamιd

2E, 7Z, 9E undecatrinic acid N-methylamide

2E, 7Z, 9E-L) -decatrienoic acid -N-ethylamide 2E, 7Z, 9E-undecatrienoic acid -Nn-propylamide 2E, 7Z, 9E undecentric acid -N-isopropylamide 2E, 7Z, 9E-undecatrienoic acid -n-n-butylamide 2E, 7Z , 9E-undecacetic acid -N- (1-methylpropyl) amide 2E, 7Z, 9E-undecatrienoic acid-N-isobutylannιd

2E, 7Z, 9E-undecatrienoic acid -N-tert-butylamide

2E, 7E, 9E undecatrinic acid -N-methyl amide 2E, 7E, 9E undecencapsic acid-N-ethyl amide 2E, 7E, 9E undecentric acid -Nn-propyl amide 2E, 7E, 9E-undecatric-N-isopropylamide 2E, 7E, 9E- Undecencenic acid -n-n-butylamide 2E, 7E, 9E-undecencentric acid-N- (1-methylpropyl) -amide 2E, 7E, 9E-undecencatenic acid-N-isobutylamide

2E, 7E, 9E-undecatrinic acid -N-tert-butylamide

2E, 7Z, 9Z-undecatrienoic acid -N-methylamide

2E, 7Z, 9Z-undecatrienoic acid -N-ethylamide 2E, 7Z, 9Z-undecatrienoic acid -N-n-propylamide 2E, 7Z, 9Z-undecatrienoic acid -N-isopropylamide

2E, 7Z, 9Z-undecatrienoic acid -N-n-butylamide

2E, 7Z, 9Z-undecatrienoic acid -N- (1-methylpropyl) -amide 2E, 7Z, 9Z-undecatrinic acid-N-isobutylamine 2E, 7Z, 9Z-undecatrienoic acid-N-tert-butylamide 2E, 7Z, 9E undecracene acid -N-methyl amide 2E, 7Z, 9E-undecatrinic acid -N-ethyl amide 2E, 7Z, 9E-L) nadecapenoic acid -Nn-propyl amide 2E, 7Z, 9E-undecrac-n-acid-N-isopropylamide 2E, 7Z, 9E-undecatrinic acid -N-n-butylamide 2E, 7Z, 9E-undecencentric acid-N- (1-methylpropyl) amide 2E, 7Z, 9E-undecacetic acid-N-isobutylamine 2E, 7Z, 9E-undecatric-N-tert-butylamide

Of course, other substitution patterns on the nitrogen atom are possible and preferred, for example, longer-chain n-alkyl radicals (-Nn-pentylamide, -Nn-pentylamide, -Nn-pentylamide, -Nn-pentylamide, -Nn-pentylamide -Nn-hexylamide, -Nn-heptylamide -Nn-octylamide, -Nn-nonylamide, -Nn-decylamide, -Nn-undecylamide, -Nn-dodecylamide, -Nn-Tπdecylamid etc) or disubstituted -N, N-Dιalkylamιde such as -N, N-dιmethylamιd, -N N - diethylamide, -N, N-d-n-propylamιd, -N, N-dιιsopropylamιd, -N, N-dι-n-butylamιd, -N, N-dι (1-methylpropyl) amιd, -N, N- dιιsobutylamιd, -N, N-dι-tert-butylamιd, -NN-methyl-ethylamide N, N-methyl-n-propylamιd, -N, N-methyl-ιsopropylamιd, -N, N-ethyl-n-propylamιd -N , N-ethyl-isopropylamide, etc

Among the compounds mentioned, some are particularly preferred in the context of the present invention. These are listed below

2E, 6Z, 8E-DecatrÃensrenic acid-N-isobutylamιd (N-isobutyl-2E, 6Z, 8E-decatrιenamιd, also called Spilanthol or Affinin)

2E, 4E, 8Z-Decatπensäure-N-ιsobutylamιd (N-isobutyl-2E, 4E, 8Z-decatrιenamιd, also called isoaffinin)

2E, 7Z, 9E-undecatrinic acid-N-isobutylamide (N-isobutyl-2E, 7Z, 9E-undecatrienamicl):

2E, 4Z-Decadienoic acid N-isobutylamide (cis-pellitorin):

2E, 4E-decadienoic acid N-isobutylamide (trans-pellitorin)

Ferulic acid amides, for example ferulic acid N-vanillylamide:

Λ / - [2- (4-Hydroxy-3-methoxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2E) -propenoic acid amide (trans -feruloylmethoxytyramine):

Λ / - [2- (4-hydroxy-3-methoxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2Z) -propenoic acid amide (cis-feruloylmethoxytyramine):

N- [1- (A-Hydroxy-3-methoxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) propanoic acid amide (dihydroferuloylmethoxytyramine):

Λ / - [2- (3,4-dihydroxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2E) - propenoic acid amide (trans -feruloyldopamine):

Λ / - [2- (3,4-Dihydroxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2Z) -propenoic acid amide (cis -feruloyldopamine):

Λ / - [2- (4-hydroxyphenyl) ethyl] -3- (3,4-dihydroxyphenyl) - (2E) propylsilamide (trans-caffeoyltyramine)

/ V- [2- (4-hydroxyphenyl) ethyl] -3- (3,4-dihydroxyphenyl) - (2Z) -propic acid amide (cis-caffeoyltyramine)

Λ / - [2- (3,4-Dιmethoxyphenyl) ethyl] -3- (3,4-dιmethoxyphenyl) - (2E) -propenoic acid (trans-Rubenamine)

/ V- [2- (3,4-dimethoxyphenyl) ethyl] -3- (3,4-dimethoxyphenyl) - (2Z) propyl acid amide (cis-Rubenamine)

Other useful in the context of the present invention Scharfstoffe are, for example, extracts of natural plants sharp-tasting plant extracts may be all physiologically acceptable vegetable extracts that cause a sharp or warm sensory impression preferred as pungent-tasting herbal extracts are pepper extract (Piper ssp, in particular Piper nigrum), water pepper extract (Polygonum ssp .inspecially Polygonum hydropiper), extracts of Allium ssp (especially onion and garlic extracts), extracts of radish (Raphanus ssp), horseradish extracts (Cochleaπa armoraαa), extracts of black (Srass / ca nigra), wild or yellow mustard {Sinapis ssp, in particular Sinapis arvensis and Sinapis alba), Bertram root extracts (Anacyclus ssp, in particular Anacyclus pyrethruml), sun hatch extracts (Echinaceae ssp), extracts of Szechuan pepper (Zanthoxylum ssp, in particular Zanthoxylum pipentum), S mushroom extract (Spilanthes ssp, in particular Spilanthes acmella), chile extract (Capsicum ssp, in particular Capsicum frutescens), Paradieskorner extract (Aframomum ssp .insbesondere Aframomum melegueta [Rose] K Schum), ginger extract [Zingiber ssp .insbesondere Zingiber officinale) and galangal extract (Kaempfena galanga or alpine galanga)

A particularly suitable substance is gingerol derived from the ginger extract

It is also possible to use N-ethyl-p-menthane-3-carboxamide (N-ethyl-5-methyl-2-isopropylcyclohexanecarboxamide)

Other pungent-tasting or heat-producing substances may be, for example, capsaiciπ, dihydrocapsaiαn, gingerol, paradol, shogaol, piperine, carboxylic acid N-vanillylamides, in particular nonanoic acid N-vanillylamide, 2-alkenic acid amides, in particular 2-nonenoic acid N- isobutylamido, 2-N-4-hydroxy-3-methoxyphenylamide, alkyl ethers of 4-hydroxy-3-methoxybenzyl alcohol, especially 4-hydroxy-3-methoxybenzyl n-butyl ether, alkyl ethers of 3-hydroxy-4-methoxybenzyl alcohol, alkyl ethers of 3,4-dimethoxybenzyl alcohol, Alkyl ethers of 3-ethoxy-4-hydroxybenzyl alcohol, alkyl ethers of 3,4-methylenedioxybenzyl alcohol, nicotinaldehyde, methyl nicotate, propyl nicotinate, 2-Butoxyethylnιcotιnat, benzyl nicotinate, 1-acetoxychavicol, polygodial or Isodrimenmol

Preferred remineralizing products according to the invention are characterized in that they contain at least one Scharfstoff from the group of N-alkyl-substituted amides of unsaturated carboxylic acids, preferably

a 2E, 6Z, 8E-Decatπensäure-N-isobutylamιd (Spilanthol) and / or b. 2E, 4E, 8Z-decatrolenic acid-N-isobutylamide and / or c. 2E, 7Z, 9E undecatrienoic acid N-isobutylamide and / or d. 2E, 4Z-decadienoic acid N-isobutylamιd (cis-pellitorin) and / or e. 2E, 4E-decadinic acid-N-isobutylamιd (trans-pellitorin) and / or f-ferulic acid-N-vanillylamide and / or g. Λ / - [2- (4-hydroxy-3-methoxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2E) - propenoic acid amide (trans -feruloylmethoxytyramine) and / or h. N- [2- (4-hydroxy-3-methoxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2Z) -propenoic acid amide (cis -feruloylmethoxytyramine) and / or i Λ / - [ 2- (4-hydroxy-3-methoxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) propanoic acid amide (dihydroferuloylmethoxytyramine) and / or j Λ / - [2- (3,4-dihydroxyphenyl) ethyl] ] -3- (4-hydroxy-3-methoxyphenyl) - (2E) - propenoic acid amide (trans -feruloyldopamine) and / or k. Λ / - [2- (3,4-dihydroxyphenyl) ethyl] -3- (4-hydroxy-3-methoxyphenyl) - (2Z) - propenoic acid amide (cis-feruloyldopamm) and / or I Λ / - [2- (4-Hydroxyphenyl) ethyl] -3- (3,4-dihydroxyphenyl) - (2E) -propenoic acid amide

(trans-caffeoyltyramine) and / or m. Λ / - [2- (4-hydroxyphenyl) ethyl] -3- (3,4-dihydroxyphenyl) - (2Z) - propenoic acid amide (cis)

Caffeoyltyramin) and / or n. / V- [2- (3,4-Dιmethoxyphenyl) ethyl] -3- (3,4-dιmethoxyphenyl) - (2E) -propenoic acid

(trans-Rubenamm) and / or o Λ / - [2- (3,4-dimethoxyphenyl) ethyl] -3- (3,4-dimethoxyphenyl) - (2Z) -propensaureamιd

(cis-Rubenamine).

In addition to or in place of the above-mentioned sharp substances, other pungent-tasting and / or a feeling of heat-generating substances may also be incorporated into the products according to the invention.

Particularly suitable within the scope of the present invention are alkyl-substituted dioxanes of the formula have been proven, in which R1 and R2 are independently selected from -H, -CH _3, -CH ₂ CH ₃ and R3 and R4 are independently selected from -H, -CH _3, -CH ₂ CH _3, -CH ₂ CH ₂ CH ₃ , - CH (CH ₃ ) ₂

As further particularly suitable in the context of the present invention phenyl esters of the formula

proved, in which R 5 is -CH ₃ or a straight-chain or branched alkyl or alkenyl radical having 2 to 8 carbon atoms, and R6 is _-CH3 or a straight-chain or branched alkyl or alkenyl radical having 2 to 8 carbon atoms or an alkoxy group having 1 to 3 Carbon atoms

Further particularly suitable within the scope of the present invention are carvone acetals of the formula

in which R 7 to R 12 are independently selected from -H, -CH ₃ , -CH ₂ CH ₃ , -CH ₂ CH ₂ CH ₃ , -CH (CH ₃ ) ₂ , -CH ₂ CH ₂ CH ₂ CH ₃ , -CH ₂ CH (CH ₃ J ₂ , -CH (CH ₃ ) CH ₂ CH ₃ -C (CH ₃ ) ₃ or R 9 and R 10 together represent a chemical bond or a group - (CR 13 R 14) -, where x is the Values 1 or 2 and R 13 and R 14 are independently selected from -H, -CH ₃ , -CH ₂ CH ₃ , -CH ₂ CH ₂ CH ₃ , -CH (CH ₃ J ₂ , -CH ₂ CH ₂ CH ₂ CH ₃ , -CH ₂ CH (CH ₃ ) ₂ , -CH (CH ₃ ) CH ₂ CH ₃ --C (CH ₃ ),

Surprisingly, it has also been found that the enzymes according to the invention are made particularly useful in liquid or semifluid dentifrice preparations especially in translucent or transparent formulations with high consumer acceptance

In a particularly preferred embodiment, the enzymes can be incorporated stably into a special matrix, the remits of corresponding agents-which can also be formulated transparently-being superior to conventional agents

Another preferred subject of the present invention is therefore an oral and dental care and -reimgungsmittel containing, based on its weight, in addition to a mixture of α-amylase and mutanase and optionally other enzymes 15 to 35 wt -% water and 35 to 55 % By weight of at least one polyhydric alcohol from the group sorbitol and / or glycene and / or 1,2-propylene glycol In addition, of course, other ingredients, as indicated above, may also be present

Erfmdungsgemaß preferred oral and Zahnpflege- and -reimgungsmittel are in this embodiment characterized in that they 20 to 34 wt -%, preferably 22.5 to 33 wt -%, more preferably 24 to 32 wt -% and in particular 25 to 31 wt - contain% water

In the calculation of the total water content of the agent, the water components of aqueous solutions are of course also included. For example, if sorbitol is used in the form of a 70% strength by weight solution, the sorbitol content is 0.7 times the weight fraction used The proportion of water is increased by 0.3 times the weight fraction used. Analogously, it is also necessary to deal with aqueous solutions of dyes or flavorings, etc.

The oral and dental care and -reimgungsmittel according to the invention contain in this preferred embodiment as a further essential ingredient at least one polyhydric alcohol from the group sorbitol, glycene and 1, 2-propylene glycol in an amount of -%

Preferred oral and dental care and cleansing compositions according to the invention are those which have 37.5 to 52.5% by weight, preferably 39 to 51% by weight, particularly preferably 40 to 50% by weight and in particular 42 to 49% by weight at least a polyhydric alcohol from the group sorbitol and / or glycene and / or 1,2-propylene glycol

Sorbitol (also referred to as glucitol) is a sugar alcohol of glucose, that is, a hexitol sorbitol can be prepared by hydrogenation of glucose, relatively easily breaks one or two molecules of water intramolecularly and forms cyclic ethers. Sorbitol comes in the form of colorless, moderately hygroscopic, optically active needles , which easily dissolve in water, in the trade Glycerol (1, 2,3-Propantrιol, 1, 2,3-Trιhydroxypropan, glycerol, oil, INCI name Glycenn, E 422) is a colorless, clear, highly mobile, odorless, sweet-tasting, hygroscopic liquid with water and Alcohol in any ratio is miscible The production of glycerol was originally as a byproduct of Fettverseifung Today's technical processes are based on propene, which is processed via the intermediates allyl chloride and epichlorohydrin to glycerol Another technical method is the hydroxylation of allyl alcohol with hydrogen peroxide at WO ₃ -Contact on the level of glycidol

1,2-propylene glycol (1,2-propanediol) is a colorless and strongly hygroscopic liquid which is miscible in any ratio with water and alcohols (such as methanol, ethanol, propanols, butanols). Technical 1,2-propanediol is a racemate of ( -) - (R) - and (+) - (S) -1, 2- Prpylenglycol The preparation is carried out by direct hydrolysis of propylene oxide As 1, 2-P further reacted with propylene oxide, thereby forming a mixture of 1, 2 and Tπpropylenglykol which must be separated by distillation 1,2-propylene glycol can also be produced from renewable raw materials via three different routes a) catalytic hydrogenation of sugars, b) fermentation of sugars to lactic acid and subsequent hydrogenation of the lactic acid ester, c) direct fermentation of sugars

For certain applications it may be advantageous to use only one of the three ingredients mentioned above. In most cases, sorbitol is preferred. However, mixtures of two of the three substances or of all three substances may be preferred in other fields of application. A mixture of glycerol is particularly advantageous , Sorbitol and 1,2-propylene glycol in a weight ratio of 1 (0.5-1) (0.1-0.5)

In addition to sorbitol or glycerol or 1, 2-propylene glycol, of which at least one substance is contained in the erfiπdungsgemaßen means, the total amount of these three substances at least 35% by weight (based on the agent), are suitable as further mhwertige alcohols such at least 2 OH groups, preferably Maπnit, xyhtol, polyethylene glycol polypropylene glycol and mixtures thereof

Among these compounds, those having 2 to 12 OH groups, and especially those having 2, 3, 4, 5, 6 or 10 OH groups are preferred

Polyhydroxy compounds having 2 OH groups are, for example, glycol (CH ₂ (OH) CH ₂ OH) and other 1, 2-diols such as H- (CH ₂ ) _n -CH (OH) CH ₂ OH with n = 2, 3, 4 , 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 18, 19, 20 Also 1,3-diols such as H- (CH ₂ ) "- CH (OH) CH ₂ CH ₂ OH with n = 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20 can be used according to the invention The (n, n + 1) - or (n, n + 2) -diols with non-terminal OH groups can also be used

Important representatives of polyhydroxy compounds having 2 OH groups are also the polyethylene and polypropylene glycols Xylitol, propylene glycols, polyethylene glycols, in particular those having average molecular weights of 200-800, can be used as preferred further polyhydric alcohols

Particularly preferred is the use of sorbitol, so that agents which contain no other polyhydric alcohols other than sorbitol, are particularly preferred

In a further particular embodiment, the agent according to the invention is a prosthesis cleaner or a denture adhesive

In addition to the already mentioned ingredients for oral, dental and / or dental prosthesis care, per-compounds such as peroxoborate, peroxomonosulfate or percarbonate are also suitable for inventively preferred prosthesis cleaners, in particular prosthesis cleansing tablets and powders Bleaching action at the same time also deodorizing and / or disinfecting The use of such per-compounds in prosthesis cleaners is between 0.01 and 10% by weight, in particular between 0.5 and 5% by weight.

The pH of the prosthesis cleaner may be between pH 4 and pH 12, in particular between pH 5 and pH 11

For the prosthesis cleansing tablets, additional auxiliaries are additionally necessary, such as, for example, agents which give rise to a bubbling effect, for example CO ₂ releasing substances such as sodium bicarbonate, fillers, for example sodium sulfate or dextrose, lubricants, for example magnesium stearate, flow control agents, for example colloidal silicon dioxide and granulating agents, such as the already mentioned high molecular weight polyethylene glycols or polyvinylpyrrolidone

Prosthesis adhesives can be offered as powders, creams, foils or liquids and support the adhesion of the prostheses Natural and synthetic swelling agents are suitable as active substances. In addition to alginates, natural swelling agents include plant gums such as gum arabic, tragacanth and karaya gum as well as natural rubber In particular, alginates and synthetic swelling agents, such as Natπumcarboxymethylcellulose, high molecular weight ethylene oxide copolymers, salts of poly (vinyl ether-co-maleic acid) and polyacrylamides have been found to be particularly suitable

Hydrophobic bases, in particular hydrocarbons, such as white Vaseline (DAB) or paraffinol, are particularly suitable as auxiliaries for pasty and liquid products Exemplary embodiments

a) enzymes

Mutanase (EC 3 2 1 59) 1200 U / g α-amylase (company Lyven, organism Bacillus subtilis, EC 3 2 1 1) 17800 U / g

b) Media

- Artificial saliva

0 1% Lab Lemco Powder, 0 2% Yeast Extract, 0 5% Peptone, 0 25% Mucin, 6 mM NaCl, 2 7 mM KCl, 3 5 mM KH 2 PO 4, 1 5 mM K 2 HPO 4, 0 05% Urea, 1 8 mM CaCl 2 , 10 μM inhibitor, pH 6 65

- Plaque culture medium

Artificial Saliva / Brain Heart Infusion Medium (Difco) (3/1) + 0.15% glucose / 0.15% sucrose

c) Verification of plaque removal

- Plaque cultivation

A mixture of the strains F nucelatum, A naeslundn, S gordonu, and S mutans were incubated for 24 h at 37 ⁰ C under anaerobic conditions in the plaque culture medium in sterile 48 well microtiter plates cultured After this time, the medium of the surfaces was removed the plaque remained on the surfaces

- Verification of plaque removal

The enzymes or enzyme mixtures were diluted in water. The following mixtures were prepared

0.25% mutanase / 0.75% amylase

0.75% mutanase / 0.25% amylase

0.25% mutanase / 0.25% amylase

0.5% mutanase / 0.5% amylase

0.5% mutanase

0.5% amylase

0.75% mutanase

0.75% amylase

1% mutanase 1% amylase 500 .mu.l of these diluted enzymes were then applied to the plaque for 1 hour at 37 ⁰ C with slight shaking movements (600 rpm Titramax 1000) Then the solution was aspirated. The remaining plaque was rinsed twice with water (1st time for 3 minutes, 600 rpm and room temperature, 2nd time for 1 minute 600 rpm and room temperature). For visualization, the remaining plaque was 0.01% Safranin O or Comassie Blue solution stained for 15 minutes Unbound dye was removed with water To determine the amount of plaque, the dye was extracted with 30% acetic acid and photometrically quantified.

Plaque Removal Results At% remaining plaque

example recipes

The following formulas are examples of compositions that may contain an enzyme mixture of α-amylase and mutanase. The amount of the components is in wt .-%, each based on the total composition.

In1 formulations:

The following commercial products were used:

Claims

claims

An oral and dental care and cleaning composition containing a mixture of an α-amylase and a mutanase.

2. Oral and dental care and cleaning agent according to claim 1, characterized in that it is the α-amylase is an α-amylase from Bacillus and / or an enzyme with a sequence homology or identity of at least 80% to that Sequence according to Swiss-Prot: P00691 acts.

3. Oral and dental care and cleaning agent according to claim 1 or 2, characterized in that it is the mutanase is a mutanase from Pseudomonas, Trichoderma, Streptomyces, Aspergillus, Flavobacteπum or Bacillus.

4. Oral and dental care and cleaning agent according to one of claims 1 to 3, characterized in that the total enzyme activity per gram oral and dental care and - cleaning agent of 0.05 to 1500 U is

5 oral and dental care and cleaning agent according to one of claims 1 to 4, characterized in that the activity ratio of α-amylase to mutanase, based on the activity units (U), from 90: 1 to 15: 1 is.

6. Oral and dental care and cleaning agent according to one of claims 1 to 5, characterized in that the wt .-% - Behhaltnιs of α-amylase to mutanase 6: 1 to 1 1.

7. Oral and dental care and cleaning agent according to one of claims 1 to 6, characterized in that the agent according to the invention in addition to the α-amylase and the mutanase contains no further enzyme

8. Oral and dental care and cleaning agent according to one of claims 1 to 6, characterized in that the inventive composition in addition to the α-amylase and the mutanase contains at least one further enzyme selected from proteases, amylases and hemicellulases.

9. Oral and dental care and cleaning agent according to claim 8, characterized in that the at least one further enzyme is selected from a ß-glucanase, a neutral protease and a corolase.

10 oral and dental care and cleaning agent according to one of claims 1 to 9, comprising, based on its weight, 15 to 35 wt -% water and 35 to 55 wt .-% at least one polyhydric alcohol from the group sorbitol and / or Glyceπn and / or 1, 2-propylene glycol

Oral and dental care and cleaning composition according to claim 10, characterized in that it contains 20 to 34% by weight, preferably 22.5 to 33% by weight, more preferably 24 to 32% by weight and in particular 25 to 31% by weight Contains water

Oral and dental care and cleaning composition according to claim 9 or 10, characterized in that it is 37.5 to 52.5% by weight, preferably 39 to 51% by weight, particularly preferably 40 to 50% by weight and in particular 42 to Contains 49% by weight of at least one polyhydric alcohol from the group of sorbitol and / or glycerm and / or 1,2-propylene glycol

Oral and dental care and cleaning agent according to one of claims 1 to 12, characterized in that the enzyme activity per gram oral and dental care and - cleaning agent 0.05 to 1500 U, preferably 0.1 to 1200 U, more preferably 0 , 25 to 1000 U and in particular 0.5 to 500 U amounts

Oral and dental care and cleaning agent according to any one of claims 1 to 13, characterized in that it additionally Putzkόrper, preferably silicic acids, aluminum hydroxide, alumina, calcium pyrophosphate, chalk, dicalcium phosphate dihydrate (CaHPO ₄ 2H ₂ O), Natπumaluminiumsilikate, in particular zeolite A, organic polymers, in particular polymethacrylates or mixtures of these Reibkorper.vorzugsweise in amounts of from 1 to 30% by weight, preferably from 2.5 to 25% by weight and in particular from 5 to 22% by weight, each based on the total agent contains

Oral and dental care and cleaning agent according to one of claims 1 to 14, characterized in that it additionally phosphate (s), preferably Alkalιmetallphosphat (s) and in particular Natπumtripolyphoyphat, preferably in amounts of 1 to 10 wt -%, more preferably of 2 to 8% by weight and in particular from 3 to 7% by weight, in each case based on the total agent

Oral and dental care and cleaning agent according to one of claims 1 to 15, characterized in that it additionally antiplaque agents, preferably p-hydroxybenzoic acid methyl, ethyl or propyl ester, Natnumsorbat, sodium benzoate, bromochlorophene, Tnclosan, phenyl salicylic acid esters, Biguanides z.sub.B chlorhexidine, thymol, preferably in amounts of from 0.1 to 5% by weight, preferably from 0.25 to 2.5% by weight and in particular from 0.5 to 1.5% by weight, in each case based on the total agent, contains Oral and dental care and cleaning agent according to one of claims 1 to 16, characterized in that it additionally Antikaπes agents, preferably Fluorverbindung (s), in particular sodium, Kaliumfluoπd, Natriummonofluorphosphat, Zinkfluoπd, Zinnfluoπd and Natπumfluorosilikat, preferably in amounts of 0 , 01 to 5 wt%, preferably from 0.1 to 2.5% by weight and in particular from 0.2 to 1.1% by weight, in each case based on the total agent

Oral and dental care and cleaning agent according to one of claims 1 to 17, characterized in that it is the insensitivity of the teeth-enhancing substances, preferably potassium salts, more preferably potassium nitrate and / or potassium citrate and / or Kaliumchloπd and / or potassium bicarbonate and / or potassium oxalate , preferably in amounts of 0.5 to 20% by weight, particularly preferably from 1, 0 to 15% by weight, more preferably from 2.5 to 10% by weight and in particular from 4.0 to 8.0% by weight, in each case to the entire agent

Oral and dental care and cleaning agent according to one of claims 1 to 18, characterized in that it contains 0.2 to 20% by weight, preferably 0.4 to 14% by weight, particularly preferably 0.5 to 3% by weight and in particular 0.6 to 2% by weight of at least one bioactive glass