EP1924698A2 - Thérapie génique oculaire utilisant la transfection médiée par avalanche - Google Patents

Thérapie génique oculaire utilisant la transfection médiée par avalanche

Info

Publication number
EP1924698A2
EP1924698A2 EP06801803A EP06801803A EP1924698A2 EP 1924698 A2 EP1924698 A2 EP 1924698A2 EP 06801803 A EP06801803 A EP 06801803A EP 06801803 A EP06801803 A EP 06801803A EP 1924698 A2 EP1924698 A2 EP 1924698A2
Authority
EP
European Patent Office
Prior art keywords
set forth
cells
tissue
nucleic acid
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP06801803A
Other languages
German (de)
English (en)
Other versions
EP1924698A4 (fr
Inventor
Thomas W. Chalberg, Jr.
Mark Blumenkranz
Daniel V. Palanker
Alexander Vankov
Philip Huie, Jr
Michael F. Marmor
Michele P. Calos
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Leland Stanford Junior University
Original Assignee
Leland Stanford Junior University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leland Stanford Junior University filed Critical Leland Stanford Junior University
Publication of EP1924698A2 publication Critical patent/EP1924698A2/fr
Publication of EP1924698A4 publication Critical patent/EP1924698A4/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/06—Antiglaucoma agents or miotics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors

Abstract

L'invention concerne un procédé permettant de traiter une maladie oculaire chez un sujet. Dans une première étape, un acide nucléique est introduit par transfection par avalanche d'électrons dans des cellules ou un tissu. Grâce à cette technique, un champ électrique élevé peut induire une bulle de plasma et une décharge de plasma entre une électrode et son milieu environnant. La formation d'une bulle de plasma génère une contrainte mécanique. La décharge de plasma réalisée à travers la vapeur ionisée dans la bulle permet une connectivité entre l'électrode et son milieu environnant, de sorte que la contrainte mécanique et le champ électrique sont appliqués simultanément, ce qui entraîne la perméabilisation des cellules ou du tissu. Cette perméabilisation permet à l'acide nucléique de pénétrer dans la cellule ou le tissu. Les cellules ou le tissu contenant l'acide nucléique sont ensuite transplantés dans une région oculaire du sujet.
EP06801803A 2005-08-15 2006-08-15 Thérapie génique oculaire utilisant la transfection médiée par avalanche Withdrawn EP1924698A4 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US70848605P 2005-08-15 2005-08-15
US36098406A 2006-02-22 2006-02-22
PCT/US2006/032249 WO2007022403A2 (fr) 2005-08-15 2006-08-15 Therapie genique oculaire utilisant la transfection mediee par avalanche

Publications (2)

Publication Number Publication Date
EP1924698A2 true EP1924698A2 (fr) 2008-05-28
EP1924698A4 EP1924698A4 (fr) 2010-12-15

Family

ID=37758427

Family Applications (1)

Application Number Title Priority Date Filing Date
EP06801803A Withdrawn EP1924698A4 (fr) 2005-08-15 2006-08-15 Thérapie génique oculaire utilisant la transfection médiée par avalanche

Country Status (5)

Country Link
EP (1) EP1924698A4 (fr)
JP (1) JP2009507780A (fr)
AU (1) AU2006279395A1 (fr)
CA (1) CA2620294A1 (fr)
WO (1) WO2007022403A2 (fr)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9226790B2 (en) 2011-02-01 2016-01-05 M.O.E. Medical Devices Llc Plasma-assisted skin treatment
US9351790B2 (en) 2011-09-17 2016-05-31 M.O.E. Medical Devices Llc Electrode geometries and method for applying electric field treatment to parts of the body

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8101169B2 (en) * 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection
WO2009062149A1 (fr) * 2007-11-08 2009-05-14 Creighton University Procédés pour conserver les neurones de l'oreille interne
WO2012086702A1 (fr) * 2010-12-24 2012-06-28 タカラバイオ株式会社 Technique d'introduction de gènes
EP2670477B1 (fr) * 2011-02-01 2015-11-25 Moe Medical Devices LLC Traitement de la peau assisté par plasma
EP3199201A1 (fr) * 2011-09-17 2017-08-02 Moe Medical Devices LLC Systeme pour champ electrique et/ou traitement de l'onychomycose assistee par plasma

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6267954B1 (en) * 1999-11-24 2001-07-31 Universite De Paris V Rene-Descartes Intraocular transplantation of encapsulated cells
US20050148530A1 (en) * 2002-02-20 2005-07-07 Sirna Therapeutics, Inc. RNA interference mediated inhibition of vascular endothelial growth factor and vascular endothelial growth factor receptor gene expression using short interfering nucleic acid (siNA)

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
BORRAS T: "RECENT DEVELOPMENTS IN OCULAR GENE THERAPY" EXPERIMENTAL EYE RESEARCH, ACADEMIC PRESS LTD, LONDON LNKD- DOI:10.1016/S0014-4835(03)00030-7, vol. 76, no. 6, 1 June 2003 (2003-06-01), pages 643-652, XP001155551 ISSN: 0014-4835 *
CHALBERG T W ET AL: "Novel physical methods for nonviral DNA transfection of RPE" IOVS, vol. 46, no. Suppl. S, 2005, page 4690, XP009130924 & ANNUAL MEETING OF THE ASSOCIATION-FOR-RESEARCH-IN-VISION-AND-OPH THALM OLOGY; FT LAUDERDALE, FL, USA; MAY 01 -05, 2005 ISSN: 0146-0404 *
CHALBERG THOMAS W ET AL: "phiC31 integrase confers genomic integration and long-term transgene expression in rat retina." INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE JUN 2005 LNKD- PUBMED:15914635, vol. 46, no. 6, June 2005 (2005-06), pages 2140-2146, XP002606111 ISSN: 0146-0404 *
MILLER JASON M ET AL: "Precision and safety of the pulsed electron avalanche knife in vitreoretinal surgery." ARCHIVES OF OPHTHALMOLOGY, vol. 121, no. 6, June 2003 (2003-06), pages 871-877, XP009130925 ISSN: 0003-9950 *
See also references of WO2007022403A2 *
SEMKOVA IRINA ET AL: "Autologous transplantation of genetically modified iris pigment epithelial cells: a promising concept for the treatment of age-related macular degeneration and other disorders of the eye." PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 1 OCT 2002 LNKD- PUBMED:12239351, vol. 99, no. 20, 1 October 2002 (2002-10-01), pages 13090-13095, XP002606109 ISSN: 0027-8424 *
TAMAI MAKOTO: "Progress in pathogenesis and therapeutic research in retinitis pigmentosa and age-related macular degeneration" NIPPON GANKA GAKKAI ZASSHI, vol. 108, no. 12, December 2004 (2004-12), pages 750-769, XP009140228 ISSN: 0029-0203 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9226790B2 (en) 2011-02-01 2016-01-05 M.O.E. Medical Devices Llc Plasma-assisted skin treatment
US9351790B2 (en) 2011-09-17 2016-05-31 M.O.E. Medical Devices Llc Electrode geometries and method for applying electric field treatment to parts of the body

Also Published As

Publication number Publication date
CA2620294A1 (fr) 2007-02-22
WO2007022403A2 (fr) 2007-02-22
AU2006279395A1 (en) 2007-02-22
EP1924698A4 (fr) 2010-12-15
WO2007022403A3 (fr) 2010-04-22
JP2009507780A (ja) 2009-02-26

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