EP1854542B1 - Filterungsvorrichtung mit mehreren Vertiefungen - Google Patents

Filterungsvorrichtung mit mehreren Vertiefungen Download PDF

Info

Publication number
EP1854542B1
EP1854542B1 EP20070107643 EP07107643A EP1854542B1 EP 1854542 B1 EP1854542 B1 EP 1854542B1 EP 20070107643 EP20070107643 EP 20070107643 EP 07107643 A EP07107643 A EP 07107643A EP 1854542 B1 EP1854542 B1 EP 1854542B1
Authority
EP
European Patent Office
Prior art keywords
filtration
plate
well
suspension
collecting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP20070107643
Other languages
English (en)
French (fr)
Other versions
EP1854542A1 (de
Inventor
Remo Anton Hochstrasser
Dieter Voegelin
Urs Schwitter
Pirmin Hidber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP06405198A external-priority patent/EP1854540A1/de
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Priority to EP20070107643 priority Critical patent/EP1854542B1/de
Publication of EP1854542A1 publication Critical patent/EP1854542A1/de
Application granted granted Critical
Publication of EP1854542B1 publication Critical patent/EP1854542B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls

Definitions

  • the present invention relates to a multi-well filtration device for filtering a suspension in general and more particularly to a system and a method for analyzing the solid phase of a suspension.
  • Suspensions basically comprise a solid phase and a liquid phase wherein the liquid phase can e.g. be a solution comprising a solute dissolved in a solvent.
  • the solution is often equilibrated at an elevated temperature close to the boiling point of the solvent.
  • the suspension is often separated into the solid phase, i.e. crystals or other solids, and into the solution.
  • microplates having a plurality of wells are common.
  • these microplates are standardized in terms of footprint dimensions, height dimensions, bottom outside flange dimensions and well positions.
  • Commonly used standardized microplates comprise 96, 384 or 1536 wells.
  • the analysis of the above mentioned solid phase i.e. crystals or other solids
  • Such analysis is preferably performed by methods like X-ray powder diffraction (XRPD) or infrared and raman spectroscopy.
  • XRPD X-ray powder diffraction
  • the crystals and solids usually have to be removed from the filtration device and be transferred into specific analysis devices, which can be a quite delicate cumbersome task.
  • Multi-well sample preparation devices including filtering of the samples are known from EP-A-1 477 812 , WO-A-01/19520 and GB-A-2 246 081 , with GB-A- 2 246 981 showing a device according to the preamble of the independent claim directed to a multi-well filtration device.
  • an improved microplate-standard compliant device being capable of separating the solid phase from the liquid phase of a suspension providing the solid phase in a manner which is easily accessible for further processing, e.g. analysis.
  • the invention deals with a multi-well filtration device for filtering a suspension, comprising a filtration plate with a number of filtration chambers and a collecting plate with a corresponding number of collecting wells.
  • Each filtration chamber is connected to a corresponding collecting well and a filter element having passages is arranged between each filtration chamber and the corresponding collecting well.
  • a separation layer is disposed between the filtration plate and the filter element.
  • the use of a separation layer allows an easy detachment of the filtration plate from the collecting plate. Since a number of filtration chambers are arranged in one single filtration plate, the separation layer ensures that the solid phase of each filtration chamber is kept separated from the solid phase of the other filtration chambers. Further, the detached filtration plate can comfortably be transferred into an analysis device, in which the solid phase of each filtration chamber can separately be analyzed through the separation layer, without removing the solid phase. Using the filtration device according to the invention, the solid phase (filter cake) of the filtration process can be analyzed without any further conditioning of the solid phase.
  • the pore size of the filter element is about 1 ⁇ m to about 2 ⁇ m.
  • the separation layer is transparent, wherein transparency relates to methods suitable for the analysis of the solid phase of a suspension, i.e. crystals or other solids. In particular, it relates to methods for the analysis of crystallized polymorphic forms of chemical compounds. Preferably, such methods are methods such as X-ray powder diffraction or infrared and Raman spectroscopy. In these cases transparent means either transparent for X-ray, for infrared light or for laser beam.
  • the separation layer preferably is made of an amorphous fluoropolymer, in particular of an amorphous fluoropolymer as it is known by persons skilled in the art as Teflon AF.
  • the separation layer has holes in an area being in contact with the filter element, said holes having a diameter larger than the diameter of the passages of the filter element.
  • Such arrangement provides a two stage filtration structure. On one stage the filtration effect is regulated by the passages of the filter element, wherein a filter cake is built during filtration. On the second stage the filter cake is held back by the separation layer inside the corresponding filtration chamber. The size of the holes is adapted to be able to hold back the filter cake without substantially effecting the filtration.
  • each collecting well in an axial cross-section has an elongated shape and a deepest portion of the collecting well is arranged at one longitudinal end region of the collecting well.
  • Elongated shape in the sense of the invention comprises all geometrical forms being suitable for the use as described below. In particular it comprises oval forms and forms of rounded rectangles suitable to gather two wells, which are arranged in a standardized microplate structure having 96, 384 or 1536 wells.
  • One advantage of the elongated shape is that supply of the suspension and extraction of the filtrate by according supply and extraction means is easily possible in each single collecting well, wherein a compact arrangement is possible. Particularly, if multiple collecting wells are arranged in one collecting plate, for example suitable for a standardized microplate comprising 96, 384 or 1536 wells, such compact arrangement can be essential.
  • the bottom of the collecting well can be slightly slanted and well rounded, such that the deepest portion of the collecting well is arranged at one longitudinal end region of the collecting well having the elongated shape, where it is accessible by extraction means.
  • the multi-well filtration device further comprises a lower funnel plate which is arranged between the filtration plate and the collecting plate.
  • the lower funnel plate has a corresponding number of filtrate funnels connecting each filtration chamber with the corresponding collecting well and the filter element is arranged at a top part of each filtrate funnel adjacent to the separation layer.
  • the filter element is thereby arranged beneath the separation layer such that it remains on the lower funnel plate being connected to the collecting plate when the filtration plate is detached and transferred.
  • the filter element is arranged as a round metal mesh being inserted into a widened top part of the filtrate funnel and being compressed with the widened top part of the corresponding filtrate funnel.
  • the metal mesh is preferably reversed around the filtrate funnel at its lateral end section such that the metal mesh is press fitted with the lower funnel plate.
  • the multi-well filtration device preferably comprises an upper funnel plate with bridging channels, wherein the filtration plate has a through holes for extraction with each through hole being connected with a respective collecting well.
  • the respective bridging channel extends through the respective through hole for extraction into the respective collecting well, such that the upper funnel plate is connected with the collecting plate via the bridging channels.
  • extraction means e.g. an extraction needle
  • the respective bridging channel extends directly into the respective collecting well, no additional sealing means have to be arranged between the upper funnel plate and the collecting plate.
  • the multi-well filtration device further comprises a top plate with needle funnels, and a pierceable septum having corresponding septum openings.
  • the septum is arranged between the top plate and the upper funnel plate or the filtration plate, respectively, such that the needle funnels are connected to a corresponding filtration chamber and that the septum openings are arranged adjacent to the respective needle funnels.
  • supply means e.g. a supply needle
  • the suspension can then be filled into the filtration chamber at an elevated pressure level driving the suspension through the filter element into the collecting well.
  • the supply means can be provided with overpressure means. While being arranged through the septum opening, the supply means are tightly connected to the septum.
  • the top plate can comprise respective second needle funnels connected to the bridging channels of the upper funnel plate.
  • extraction means e.g. an extraction needle
  • the top plate can have a recess around each needle funnel on the side facing the septum, and the upper funnel plate or the filtration plate, respectively, can have a corresponding ridge on the side facing the septum, such that upon assembly the septum is pressed into the recess by the ridge.
  • the arrangement of the recess and the ridge can as well be vice versa, i.e. the top plate having the ridge and the upper funnel plate or the filtration plate, respectively, having the recess. With such an arrangement, it is possible to ensure a tight connection between the septum and its adjacent layers in its essential region, i.e. around the needle funnel, such that it is possible to provide an elevated pressure in the filtration chamber.
  • the multi-well filtration device further comprises pressure equalization channels for equalizing the pressure in each collecting well, such that the supply of the suspension into the filtration chambers is not obstructed by an increasing pressure in the collecting wells.
  • the pressure equalization channel can easily be arranged between said bridging channel and said through hole.
  • a sealing mat is arranged between two adjacent plates, said sealing mat having holes being located corresponding to adjacent openings of the two plates.
  • One of said two plates has a sealing ridge on the side facing the sealing mat thus being capable of receiving the border of the hole on top of the sealing ridge.
  • a second aspect of the invention deals with a system for analyzing the solid phase of a suspension, comprising the multi-well filtration device described above.
  • the system further comprises a number of supply needles for supplying the suspension into the corresponding number of filtration chambers at an elevated pressure level and a corresponding number of extraction needles for extracting filtrate out of the corresponding number of collecting wells.
  • it comprises an analysis device arranged for analyzing the solid phase of the suspension filtered by the multi-well filtration device through the separation layer, while the respective filter cake of the solid phase of the suspension is situated in the respective filtration chambers of the filtration plate of the multi-well filtration device.
  • the separation layer is transparent as described above.
  • each supply needle has a longitudinal groove for performing equalization of pressure in the respective collecting well while the supply needle is supplying the suspension into the corresponding filtration chamber.
  • a groove can be connected to pressure equalization means of the multi-well filtration device, e.g. a pressure equalization channel, such that the pressure in the collecting well and the filtration chamber can easily be equalized.
  • a third aspect of the invention deals with a method for analyzing the solid phase of a suspension by means of an analyzing system described above.
  • the method comprises the steps of: supplying the suspension into the filtration chambers at an elevated pressure level by means of the respective supply needles thereby driving the suspension through the filter element into the collecting wells; removing the filtration plate together with the separation layer from the collecting plate; transferring the filtration plate with the filter cakes of the solid phase of the suspension contained in the respective filtration chambers to the analysis device; and analyzing the solid phase through the transparent separation layer.
  • Fig. 1 shows a top view on a multi-well filtration device according to the invention
  • Fig. 2 shows a cross-sectional view along the line A-A of the multi-well filtration device of Fig. 1 ;
  • Fig. 3 shows an exploded view of the cross-sectional view of Fig. 2 ;
  • Fig. 4 shows an expanded view of a part of the cross-sectional view of Fig. 2 , where a supply needle penetrates a septum;
  • Fig. 5 shows a two-step stair-shaped elevation for receiving a sealing mat of the multi-well filtration device from Fig. 1 ;
  • Fig. 6 shows an exploded perspective view of a transfer unit for the multi-well filtration device from Fig. 1 ;
  • Fig. 7 shows a top view on the transfer unit from Fig. 6 ;
  • Fig. 8 shows a exploded cross-section view along the line A-A of the transfer unit from Fig. 7 .
  • Fig. 1 shows a top view on a top plate 1 of a multi-well filtration device according to the present invention.
  • the top plate 1 comprises 96 needle funnels for supply 11 and 96 needle funnels for extraction 13.
  • one of the needle funnels for supply 11 is equipped with a supply needle 91 and one of the needle funnels for extraction 13 is equipped with an extraction needle 92.
  • Fig. 1 at the right hand side and at the above side of the top plate 1 the top side of a filtration plate 4 is visible.
  • the filtration plate 4 is arranged below the top plate 1 as shown in Fig. 2 and Fig. 3 .
  • the top plate 1, the needle funnels for supply 11 and the needle funnels for extraction 13 are arranged in a standardized 96 wells microplate compliant structure.
  • standardized microplate compliant structures allow the use of the multi-well filtration device in a standardized infrastructure.
  • standardized liquid handling and analysis devices can be used.
  • Fig. 2 and Fig. 3 show a cross-sectional view or an exploded cross-sectional view, respectively, of the multi-well filtration device along the line A-A of Fig. 1 .
  • the multi-well filtration device comprises a septum 2 being arranged between an upper funnel plate 3 and the top plate 1.
  • the upper funnel plate 3 is followed from top to bottom by: a first sealing mat 6; a filtration plate 4; a second sealing mat 6; a transparent separation layer 5; a third sealing mat 6; a lower funnel plate 7; a fourth sealing mat 6; and a collecting plate 8.
  • the upper funnel plate 3 has alternatingly arranged through holes for supply 33 and bridging channels 31 being interconnected by pressure equalization channels 34.
  • Each through hole for supply 33 of the upper funnel plate 3 is connected to one of the needle funnels for supply 11 of the top plate 1 via a septum opening 21 of the septum 2.
  • each bridging channel 31 of the upper funnel plate 3 is connected to one of the needle funnels for extraction 13 of the top plate 1 via a septum opening 21 of the septum 2.
  • the top plate 1 has recesses 12 around the needle funnels for supply 11 and around the needle funnels for extraction 13 on its under side and the upper funnel plate 3 has ridges 32 around the through holes for supply 33 and the bridging channels 31 on its upper side.
  • the septum 2 is pressed into the recesses 12 by the ridges 32.
  • the filtration plate 4 has alternatingly arranged through holes for extraction 42 and filtration chambers 41, wherein each filtration chamber 41 is connected to one of the through holes for supply 33 of the upper funnel plate 3.
  • Each bridging channel 31 extends through one of the through holes for extraction 42 of the filtration plate 4 projecting below the filtration plate 4.
  • the filtration plate 4 is connected to the transparent separation layer 5, being again connected to the lower funnel plate 7, wherein a sealing mat 6 is arranged between the transparent separation layer 5 and the lower funnel plate 7.
  • the lower funnel plate 7 has alternatingly arranged through holes for extraction 72 and filtrate funnels 71, wherein each filtrate funnel 71 is connected to one of the filtration chambers 41.
  • Each bridging channel 31 of the upper funnel plate 3 extends again through one of the through holes for extraction 72.
  • each filtrate funnel 71 is equipped with a filter element 73 having passages of a certain diameter.
  • the transparent separation layer 5 has holes (not shown in the figures) having a diameter larger than the diameter of the passages of the filter elements 73.
  • each of the filter elements 73 is arranged as a round metal mesh being inserted into a widened top part of the corresponding filtrate funnel 71 and being compressed with the widened top part of the corresponding filtrate funnel 71.
  • each metal mesh is preferably reversed around the filtrate funnel 71 at its lateral end section such that the metal mesh is press fitted with the lower funnel plate 7.
  • the pore size of the filter element is about 1 ⁇ m to about 2 ⁇ m.
  • the lower funnel plate 7 is connected to the collecting plate 8, wherein a sealing mat 6 is arranged in-between.
  • the collecting plate 8 has collecting wells 81 with elongated cross-sections having the form of rounded rectangles. Each of said collecting wells 81 is connected to one filtrate funnel 71 of the lower funnel plate 7 and to one bridging channel 31 of the upper funnel plate 3.
  • the bottoms of the collecting wells 81 are slightly slanted and well rounded, wherein each collecting well 81 has a deepest point 811 lying essentially straight below the bridging channel 31 being connected to said collecting well 81.
  • one of the septum openings 21 being connected to one of the filtration chambers 41 is penetrated by a supply needle 91, such that the supply needle 91 extends into said filtration chamber 41.
  • the supply needle 91 has a tapered portion for accommodating the septum opening 21 of the septum 2.
  • the supply needle 91 supplies a suspension into the filtration chamber 41 thereby creating an overpressure inside the filtration chamber 41 in order to drive the suspension through the filter element 73 into the collecting well 81.
  • the overpressure can be provided by pressure means of the supply needle 91.
  • said overpressure provision for driving the filtration has the advantage that crystal formation in the suspension is low compared to driving filtration by creating a vacuum in the collecting well 81. Therefore it is possible to get a filtrate with a comparably high concentration of solute without interfering seeds.
  • parts of the multi-well filtration device being possibly in contact with the suspension are preferably made of an isolating material, such that the cooling of the suspension being filtered at an elevated temperature is as low as possible.
  • the supply needle 91 has a longitudinal groove being connected to the according pressure equalization channel 92.
  • the pressure can be equalized between the collecting well 81 and the air pressure outside the multi-well filtration device.
  • the filter element 73 retains solids of the supplied suspension, which are not able to pass the passages. Thereby a filter cake is built on top of the transparent separation layer 5.
  • the diameter of the holes of the transparent separation layer 5 is large enough not to essentially effect the filtration and in the meantime it is small enough to be able to hold back the filter cake.
  • the septum opening 21 being connected to the according bridging channel 31 is penetrated by an extraction needle 92, such that it extends near the deepest point 811 of the bottom of the collecting well 81. Since the bottom of the collecting well 81 is slightly slanted and well rounded, the filtrate can then efficiently be extracted preventing a comparably high dead volume of filtrate in the collecting well 81.
  • the filtration plate 4 can easily be separated from the lower funnel plate 7 by means of the transparent separation layer 5.
  • the filter cake containing crystals and other solids to be analyzed is still held inside the filtration chambers 41 by the transparent separation layer 5. Without any laborious preparation steps the filter cake can be transferred into an analysis device and it can be analyzed through the transparent separation layer 5 by an appropriate analysis method, such as X-ray powder diffraction or infrared and Raman spectroscopy.
  • Fig. 5 shows a two-step stair-shaped elevation for receiving a sealing mat 6.
  • the border around one of the two openings has preferably a two-step stair-shaped elevation.
  • the sealing mat 6 is lifted and arranged on top of a sealing ridge 74 touching a guiding ridge 75.
  • the sealing mat 6 is lifted around the opening and when the two layers are connected it is compressed in said lifted area.
  • a transfer unit comprising the filtration plate 4 with the two adjacent sealing mats 6 and the separation layer 5.
  • a closing layer 5A followed by a top plate 1A are arranged on top of the upper sealing mat 6 .
  • a closing layer 5B followed by a bottom plate 1B are arranged below the separation layer 5.
  • the filtration layer 4 together with the two adjacent sealing mats 6 and the separation layer 5 can easily be separated from rest of the multi-well filtration device as described above.
  • the filtration plate 4 can then be closed as well as at its upper surface as at its bottom surface by closing layers 5A and 5B followed by a top plate 1A or a bottom plate, respectively.
  • the top plate 1A can be firmly connected to the bottom plate 1B in order to form a compact transfer unit.
  • the top plate 1A is provided with screw holes 12A
  • the filtration plate 4 is provided with screw holes 43
  • the bottom plate 1B is provided with screw holes 12B, such that the top plate 1A can be firmly connected to the bottom plate 1B by means of screws extending through said screw holes 12 A, 43 and 12B.
  • the transfer unit still comprising the filter cakes inside the filtration chambers 41 of the filtration plate 4, can then be comfortably moved, stored or transferred to the according analysis device.
  • the upper plate 1A and the bottom plate 1B are provided with through holes 11A and 11B being arranged adjacent to the filtration chambers 41 as well as the closing layers 5A and 5B are made of a transparent material, such that the filter cakes can be analyzed through the through holes 11A and 11B and the closing layers 5A and 5B by an appropriate analysis method, such as X-ray powder diffraction or infrared and Raman spectroscopy.

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (11)

  1. Mehrloch-Filtrationsvorrichtung zum Filtrieren einer Suspension, umfassend eine Filtrationsplatte (4) mit einer Anzahl Filtrationskammern (41) und einer Sammelplatte (8) mit einer entsprechenden Zahl von Auffangvertiefungen (81), wobei jede Filtrationskammer (41) mit einer entsprechenden Auffangvertiefung (81) verbunden ist und wobei ein Filterelement (73) mit Durchgängen zwischen jeder Filtrationskammer (41) und der entsprechenden Auffangvertiefung (81) angeordnet ist, dadurch gekennzeichnet, dass eine Trennschicht (5) zwischen der Filtrationsplatte (4) und dem Filterelement (73) angeordnet ist, diese Trennschicht (5) durchsichtig ist und Löcher in einem Bereich hat, der in Kontakt mit dem Filterelement ist, wobei diese Löcher einen Durchmesser haben, der grösser ist als der Durchmesser der Durchgänge des Filterelements und der dazu angepasst ist, einen Filterkuchen einer festen Phase der Suspension in jeder Filtrationskammer zurückhalten zu können ohne die Filtration wesentlich zu bewirken.
  2. Mehrloch-Filtrationsvorrichtung gemäss Anspruch 1, wobei jede Auffangvertiefung (81) in einem axialen Querschnitt eine längliche Form hat und wobei ein tiefster Teilbereich (811) der Auffangvertiefung (81) an einem längsseitigen Endbereich der Auffangvertiefung angeordnet ist.
  3. Mehrloch-Filtrationsvorrichtung gemäss einem der Ansprüche 1 oder 2, ferner umfassend eine untere Trichterplatte (7), die zwischen der Filtrationsplatte (4) und der Sammelplatte (8) angeordnet ist, wobei die untere Trichterplatte (7) eine entsprechende Zahl Filtrattrichter (71) hat, welche jede Filtrationskammer (41) mit der entsprechenden Auffangvertiefung (81) verbinden, und wobei das Filterelement (73) in einem obersten Teil jedes Filtrattrichters (71) benachbart zur Trennschicht angeordnet ist.
  4. Mehrloch-Filtrationsvorrichtung gemäss einem der Ansprüche 1 bis 3, ferner umfassend eine obere Trichterplatte (3) mit Überbrückungskanälen (31), wobei die Filtrationsplatte (4) Durchgangslöcher zur Extraktion (42) hat und jedes Durchgangsloch mit einer entsprechenden Auffangvertiefung (81) verbunden ist, und wobei der jeweilige Überbrückungskanal (31) sich durch das entsprechende Durchgangsloch zur Extraktion (42) in die entsprechende Auffangvertiefung (81) hinein erstreckt, so dass die obere Trichterplatte (3) mit der Sammelplatte (8) über die Überbrückungskanäle (31) verbunden ist.
  5. Mehrloch-Filtrationsvorrichtung gemäss einem der Ansprüche 1 bis 4, ferner umfassend eine oberste Platte (1) mit Nadeltrichtern (11, 13) und einem durchstossbaren Septum (2) mit entsprechenden Septumöffnungen (21), wobei das Septum (2) zwischen der obersten Platte (1) und der oberen Trichterplatte (3) bzw. der Filtrationsplatte angeordnet ist, so dass die Nadeltrichter (11, 13) mit einer entsprechenden Filtrationskammer (41) verbunden sind und dass die Septumöffnungen (21) benachbart zu den Nadeltrichtern (11, 13) angeordnet sind.
  6. Mehrloch-Filtrationsvorrichtung gemäss Anspruch 5, wobei die oberste Platte (1) eine Vertiefung (12) um jeden Nadeltrichter (11, 13) herum auf der dem Septum (2) zugewandten Seite hat, und wobei die obere Trichterplatte (3) oder die Filtrationsplatte einen entsprechende Kante (32) auf der dem Septum (2) zugewandten Seite hat, so dass beim Zusammenbau das Septum (2) durch die Kante (32) in die Vertiefung (12) gepresst wird.
  7. Mehrloch-Filtrationsvorrichtung gemäss einem der Ansprüche 1 bis 6, ferner umfassend Druckausgleichskanäle (34) zum Ausgleichen des Drucks in jeder Auffangvertiefung (81).
  8. Mehrloch-Filtrationsvorrichtung gemäss einem der Ansprüche 1 bis 7, wobei eine Dichtmatte (6) zwischen zwei benachbarten Platten angeordnet ist, wobei diese Dichtmatte (6) Löcher hat, die entsprechend den benachbarten Öffnungen der beiden Platten angeordnet sind, und wobei eine dieser zwei Platten eine Dichtkante (74) auf der der Dichtmatte (6) zugewandten Seite hat, so dass diese den Rand des Lochs oberhalb der Dichtkante (74) aufnehmen kann.
  9. System zur Analyse der festen Phase einer Suspension, umfassend eine Anzahl Zuführnadeln (91) zum Zuführen der Suspension in eine entsprechende Zahl von Filtrationskammern (41) bei einem erhöhten Druckniveau, eine entsprechende Zahl von Extraktionsnadeln (92) zum Extrahieren von Filtrat aus einer entsprechenden Zahl von Auffangvertiefungen (81), und eine Analyse-Vorrichtung zum Analysieren einer festen Phase der Suspension, die durch eine Mehrloch-Filtrationsvorrichtung filtriert wird, gekennzeichnet durch eine Mehrloch-Filtrationsvorrichtung gemäss einem der Ansprüche 1 bis 8, und dadurch, dass der entsprechende Filterkuchen der festen Phase der Suspension mittels der Analyse-Vorrichtung durch die Trennschicht (5) analysiert wird, während der Filterkuchen sich in der entsprechenden Filtrationskammer (41) der Filtrationsplatte (4) der Mehrloch-Filtrationsvorrichtung befindet.
  10. System gemäss Anspruch 9, wobei jede Zuführnadel (91) eine Längsnut aufweist zum Durchführen des Druckausgleichs in der entsprechenden Auffangvertiefung, während die Zuführnadel (91) die Suspension in die entsprechende Filtrationskammer (41) hinein zuführt.
  11. Verfahren zur Analyse der festen Phase einer filtrierten Suspension, umfassend die Schritte:
    Einbringen einer Suspension in eine Anzahl Filtrationskammern (41),
    Durchdrücken der Suspension durch ein Filterelement (73) in eine entsprechende Zahl von AufFangvertiefungen (81) hinein, und
    Analysieren einer vom Filterelement (73) zurückgehaltenen festen Phase der Suspension, gekennzeichnet durch
    Verwenden eines Systems gemäss Anspruch 8 oder Anspruch 9, wobei die Suspension in die entsprechende Filtrationskammer (41) der Filtrationsplatte (4) unter erhöhtem Druckniveau mittels einer entsprechenden Zuführnadel (91) eingebracht wird,
    nach der Filtration Entfernen der Filtrationsplatte (4) zusammen mit der Trennschicht (5) von der Sammelplatte (8), Überführen der Filtrationsplatte (4) mit einem in der entsprechenden Filtrationskammer enthaltenen Filterkuchen der festen Phase der Suspension zur Analyse-Vorrichtung, und
    Analysieren des entsprechenden Filterkuchens durch die Trennschicht (5).
EP20070107643 2006-05-12 2007-05-07 Filterungsvorrichtung mit mehreren Vertiefungen Active EP1854542B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20070107643 EP1854542B1 (de) 2006-05-12 2007-05-07 Filterungsvorrichtung mit mehreren Vertiefungen

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06405198A EP1854540A1 (de) 2006-05-12 2006-05-12 Multiwell Filtrationsvorrichtung
EP20070107643 EP1854542B1 (de) 2006-05-12 2007-05-07 Filterungsvorrichtung mit mehreren Vertiefungen

Publications (2)

Publication Number Publication Date
EP1854542A1 EP1854542A1 (de) 2007-11-14
EP1854542B1 true EP1854542B1 (de) 2011-03-30

Family

ID=38573168

Family Applications (1)

Application Number Title Priority Date Filing Date
EP20070107643 Active EP1854542B1 (de) 2006-05-12 2007-05-07 Filterungsvorrichtung mit mehreren Vertiefungen

Country Status (1)

Country Link
EP (1) EP1854542B1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3655128B1 (de) * 2017-09-29 2023-08-30 Sartorius Stedim Biotech GmbH Filtrationsvorrichtung, verfahren zum zusammenfügen einer modularen filtrationsvorrichtung sowie verfahren zur charakterisierung eines filtermediums und/oder zu filtrierenden mediums

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1854540A1 (de) * 2006-05-12 2007-11-14 F. Hoffmann-la Roche AG Multiwell Filtrationsvorrichtung
CN102665913B (zh) * 2009-07-31 2015-11-25 西蒙·斯塔福德 用于微量培养板中的改进的液体操纵的装置
DE102010011485A1 (de) 2010-03-16 2011-09-22 Sartorius Stedim Biotech Gmbh Mehrfachlochplatte mit Filtermedium und ihre Verwendung

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141719A (en) * 1990-07-18 1992-08-25 Bio-Rad Laboratories, Inc. Multi-sample filtration plate assembly
US5972694A (en) * 1997-02-11 1999-10-26 Mathus; Gregory Multi-well plate
US6159368A (en) * 1998-10-29 2000-12-12 The Perkin-Elmer Corporation Multi-well microfiltration apparatus
EP1227888A4 (de) * 1999-09-13 2006-05-24 Millipore Corp Vor ort gegossene karte von hoher dichte zur probenvorbereitung
US20030143124A1 (en) * 2002-01-31 2003-07-31 Roberts Roger Q. Unidirectional flow control sealing matt
AU2003267077A1 (en) * 2002-11-12 2004-06-03 Millipore Corporation Evaporation control device for multiwell plates
CA2467131C (en) * 2003-05-13 2013-12-10 Becton, Dickinson & Company Method and apparatus for processing biological and chemical samples

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3655128B1 (de) * 2017-09-29 2023-08-30 Sartorius Stedim Biotech GmbH Filtrationsvorrichtung, verfahren zum zusammenfügen einer modularen filtrationsvorrichtung sowie verfahren zur charakterisierung eines filtermediums und/oder zu filtrierenden mediums

Also Published As

Publication number Publication date
EP1854542A1 (de) 2007-11-14

Similar Documents

Publication Publication Date Title
US7867452B2 (en) Multi-well filtration device
US20220118455A1 (en) Digital microfluidic devices and methods
EP0591436B1 (de) Vorrichtung zur kultivierung und manipulierung von zellen
DE69913978T2 (de) Multiprobenbehältermikrofiltrationsvorrichtung
EP1854542B1 (de) Filterungsvorrichtung mit mehreren Vertiefungen
US8007743B2 (en) Multifunctional vacuum manifold
DE60317603T2 (de) Mikroplatte mit Schutzunterplatte
EP1383604B1 (de) Muldenplatten-dichtungsmatte für unidirektionale strömungssteuerung
US20060191893A1 (en) Manually-operable multi-well microfiltration apparatus and method
EP1724019B1 (de) Aufnahmeplatte mit mehreren Querschnitten
DE202011110979U1 (de) System zum Bilden von Emulsionen
CN101223101A (zh) 具有集成的微型泵尤其是生化微反应器的微流体装置及其制造方法
EP1366819B1 (de) Einteilige Filterplatte
JP2003530991A (ja) 精製装置及び方法
AU2003210528A1 (en) Unidirectional flow control sealing matt for well plate
US20020150505A1 (en) Manually-operable multi-well microfiltration apparatus and method
EP1151794A2 (de) Bodenabfluss für Filtermembran
EP2758764B1 (de) Festphasenextraktionsvorrichtung für getrocknete von probenkarten
DE602004011647T2 (de) Schicht und Abstehrippen für die Abflussmatte einer Mehrlochtestplatte
JP4794582B2 (ja) 多機能真空マニフォールド
US20030202909A1 (en) Vacuum manifold and uses thereof
CN212871934U (zh) 多孔板过滤装置
CN112067412A (zh) 多孔板过滤装置及方法
WO2024214671A1 (ja) 生体関連物質の溶液採取装置
EP1547690B1 (de) Verfahren zur Befestigung einer Membran mittels Heissschmelzen ohne Klebstoff

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA HR MK YU

17P Request for examination filed

Effective date: 20080503

17Q First examination report despatched

Effective date: 20080604

AKX Designation fees paid

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: CH

Ref legal event code: EP

REG Reference to a national code

Ref country code: CH

Ref legal event code: NV

Representative=s name: BOHEST AG

REG Reference to a national code

Ref country code: IE

Ref legal event code: FG4D

REF Corresponds to:

Ref document number: 602007013487

Country of ref document: DE

Date of ref document: 20110512

Kind code of ref document: P

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602007013487

Country of ref document: DE

Effective date: 20110512

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2362129

Country of ref document: ES

Kind code of ref document: T3

Effective date: 20110628

REG Reference to a national code

Ref country code: NL

Ref legal event code: VDEP

Effective date: 20110330

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LV

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: GR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110701

Ref country code: SE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: LT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

LTIE Lt: invalidation of european patent or patent extension

Effective date: 20110330

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: CY

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: AT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: SI

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: EE

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: PT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110801

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: RO

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: CZ

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: IS

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110730

Ref country code: SK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: MT

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: NL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: MC

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20110531

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: DK

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

Ref country code: PL

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

REG Reference to a national code

Ref country code: IE

Ref legal event code: MM4A

26N No opposition filed

Effective date: 20120102

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602007013487

Country of ref document: DE

Effective date: 20120102

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20110507

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: LU

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20110507

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: BG

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110630

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: TR

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: HU

Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

Effective date: 20110330

REG Reference to a national code

Ref country code: CH

Ref legal event code: PCAR

Free format text: NEW ADDRESS: HOLBEINSTRASSE 36-38, 4051 BASEL (CH)

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 10

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 11

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 12

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20230510

Year of fee payment: 17

Ref country code: ES

Payment date: 20230608

Year of fee payment: 17

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20240418

Year of fee payment: 18

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20240418

Year of fee payment: 18

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: CH

Payment date: 20240602

Year of fee payment: 18

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: FR

Payment date: 20240418

Year of fee payment: 18