EP1854542B1 - Dispositif filtrant multipuits - Google Patents

Dispositif filtrant multipuits Download PDF

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Publication number
EP1854542B1
EP1854542B1 EP20070107643 EP07107643A EP1854542B1 EP 1854542 B1 EP1854542 B1 EP 1854542B1 EP 20070107643 EP20070107643 EP 20070107643 EP 07107643 A EP07107643 A EP 07107643A EP 1854542 B1 EP1854542 B1 EP 1854542B1
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EP
European Patent Office
Prior art keywords
filtration
plate
well
suspension
collecting
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EP20070107643
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German (de)
English (en)
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EP1854542A1 (fr
Inventor
Remo Anton Hochstrasser
Dieter Voegelin
Urs Schwitter
Pirmin Hidber
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Priority claimed from EP06405198A external-priority patent/EP1854540A1/fr
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Priority to EP20070107643 priority Critical patent/EP1854542B1/fr
Publication of EP1854542A1 publication Critical patent/EP1854542A1/fr
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0848—Specific forms of parts of containers
    • B01L2300/0851—Bottom walls

Definitions

  • the present invention relates to a multi-well filtration device for filtering a suspension in general and more particularly to a system and a method for analyzing the solid phase of a suspension.
  • Suspensions basically comprise a solid phase and a liquid phase wherein the liquid phase can e.g. be a solution comprising a solute dissolved in a solvent.
  • the solution is often equilibrated at an elevated temperature close to the boiling point of the solvent.
  • the suspension is often separated into the solid phase, i.e. crystals or other solids, and into the solution.
  • microplates having a plurality of wells are common.
  • these microplates are standardized in terms of footprint dimensions, height dimensions, bottom outside flange dimensions and well positions.
  • Commonly used standardized microplates comprise 96, 384 or 1536 wells.
  • the analysis of the above mentioned solid phase i.e. crystals or other solids
  • Such analysis is preferably performed by methods like X-ray powder diffraction (XRPD) or infrared and raman spectroscopy.
  • XRPD X-ray powder diffraction
  • the crystals and solids usually have to be removed from the filtration device and be transferred into specific analysis devices, which can be a quite delicate cumbersome task.
  • Multi-well sample preparation devices including filtering of the samples are known from EP-A-1 477 812 , WO-A-01/19520 and GB-A-2 246 081 , with GB-A- 2 246 981 showing a device according to the preamble of the independent claim directed to a multi-well filtration device.
  • an improved microplate-standard compliant device being capable of separating the solid phase from the liquid phase of a suspension providing the solid phase in a manner which is easily accessible for further processing, e.g. analysis.
  • the invention deals with a multi-well filtration device for filtering a suspension, comprising a filtration plate with a number of filtration chambers and a collecting plate with a corresponding number of collecting wells.
  • Each filtration chamber is connected to a corresponding collecting well and a filter element having passages is arranged between each filtration chamber and the corresponding collecting well.
  • a separation layer is disposed between the filtration plate and the filter element.
  • the use of a separation layer allows an easy detachment of the filtration plate from the collecting plate. Since a number of filtration chambers are arranged in one single filtration plate, the separation layer ensures that the solid phase of each filtration chamber is kept separated from the solid phase of the other filtration chambers. Further, the detached filtration plate can comfortably be transferred into an analysis device, in which the solid phase of each filtration chamber can separately be analyzed through the separation layer, without removing the solid phase. Using the filtration device according to the invention, the solid phase (filter cake) of the filtration process can be analyzed without any further conditioning of the solid phase.
  • the pore size of the filter element is about 1 ⁇ m to about 2 ⁇ m.
  • the separation layer is transparent, wherein transparency relates to methods suitable for the analysis of the solid phase of a suspension, i.e. crystals or other solids. In particular, it relates to methods for the analysis of crystallized polymorphic forms of chemical compounds. Preferably, such methods are methods such as X-ray powder diffraction or infrared and Raman spectroscopy. In these cases transparent means either transparent for X-ray, for infrared light or for laser beam.
  • the separation layer preferably is made of an amorphous fluoropolymer, in particular of an amorphous fluoropolymer as it is known by persons skilled in the art as Teflon AF.
  • the separation layer has holes in an area being in contact with the filter element, said holes having a diameter larger than the diameter of the passages of the filter element.
  • Such arrangement provides a two stage filtration structure. On one stage the filtration effect is regulated by the passages of the filter element, wherein a filter cake is built during filtration. On the second stage the filter cake is held back by the separation layer inside the corresponding filtration chamber. The size of the holes is adapted to be able to hold back the filter cake without substantially effecting the filtration.
  • each collecting well in an axial cross-section has an elongated shape and a deepest portion of the collecting well is arranged at one longitudinal end region of the collecting well.
  • Elongated shape in the sense of the invention comprises all geometrical forms being suitable for the use as described below. In particular it comprises oval forms and forms of rounded rectangles suitable to gather two wells, which are arranged in a standardized microplate structure having 96, 384 or 1536 wells.
  • One advantage of the elongated shape is that supply of the suspension and extraction of the filtrate by according supply and extraction means is easily possible in each single collecting well, wherein a compact arrangement is possible. Particularly, if multiple collecting wells are arranged in one collecting plate, for example suitable for a standardized microplate comprising 96, 384 or 1536 wells, such compact arrangement can be essential.
  • the bottom of the collecting well can be slightly slanted and well rounded, such that the deepest portion of the collecting well is arranged at one longitudinal end region of the collecting well having the elongated shape, where it is accessible by extraction means.
  • the multi-well filtration device further comprises a lower funnel plate which is arranged between the filtration plate and the collecting plate.
  • the lower funnel plate has a corresponding number of filtrate funnels connecting each filtration chamber with the corresponding collecting well and the filter element is arranged at a top part of each filtrate funnel adjacent to the separation layer.
  • the filter element is thereby arranged beneath the separation layer such that it remains on the lower funnel plate being connected to the collecting plate when the filtration plate is detached and transferred.
  • the filter element is arranged as a round metal mesh being inserted into a widened top part of the filtrate funnel and being compressed with the widened top part of the corresponding filtrate funnel.
  • the metal mesh is preferably reversed around the filtrate funnel at its lateral end section such that the metal mesh is press fitted with the lower funnel plate.
  • the multi-well filtration device preferably comprises an upper funnel plate with bridging channels, wherein the filtration plate has a through holes for extraction with each through hole being connected with a respective collecting well.
  • the respective bridging channel extends through the respective through hole for extraction into the respective collecting well, such that the upper funnel plate is connected with the collecting plate via the bridging channels.
  • extraction means e.g. an extraction needle
  • the respective bridging channel extends directly into the respective collecting well, no additional sealing means have to be arranged between the upper funnel plate and the collecting plate.
  • the multi-well filtration device further comprises a top plate with needle funnels, and a pierceable septum having corresponding septum openings.
  • the septum is arranged between the top plate and the upper funnel plate or the filtration plate, respectively, such that the needle funnels are connected to a corresponding filtration chamber and that the septum openings are arranged adjacent to the respective needle funnels.
  • supply means e.g. a supply needle
  • the suspension can then be filled into the filtration chamber at an elevated pressure level driving the suspension through the filter element into the collecting well.
  • the supply means can be provided with overpressure means. While being arranged through the septum opening, the supply means are tightly connected to the septum.
  • the top plate can comprise respective second needle funnels connected to the bridging channels of the upper funnel plate.
  • extraction means e.g. an extraction needle
  • the top plate can have a recess around each needle funnel on the side facing the septum, and the upper funnel plate or the filtration plate, respectively, can have a corresponding ridge on the side facing the septum, such that upon assembly the septum is pressed into the recess by the ridge.
  • the arrangement of the recess and the ridge can as well be vice versa, i.e. the top plate having the ridge and the upper funnel plate or the filtration plate, respectively, having the recess. With such an arrangement, it is possible to ensure a tight connection between the septum and its adjacent layers in its essential region, i.e. around the needle funnel, such that it is possible to provide an elevated pressure in the filtration chamber.
  • the multi-well filtration device further comprises pressure equalization channels for equalizing the pressure in each collecting well, such that the supply of the suspension into the filtration chambers is not obstructed by an increasing pressure in the collecting wells.
  • the pressure equalization channel can easily be arranged between said bridging channel and said through hole.
  • a sealing mat is arranged between two adjacent plates, said sealing mat having holes being located corresponding to adjacent openings of the two plates.
  • One of said two plates has a sealing ridge on the side facing the sealing mat thus being capable of receiving the border of the hole on top of the sealing ridge.
  • a second aspect of the invention deals with a system for analyzing the solid phase of a suspension, comprising the multi-well filtration device described above.
  • the system further comprises a number of supply needles for supplying the suspension into the corresponding number of filtration chambers at an elevated pressure level and a corresponding number of extraction needles for extracting filtrate out of the corresponding number of collecting wells.
  • it comprises an analysis device arranged for analyzing the solid phase of the suspension filtered by the multi-well filtration device through the separation layer, while the respective filter cake of the solid phase of the suspension is situated in the respective filtration chambers of the filtration plate of the multi-well filtration device.
  • the separation layer is transparent as described above.
  • each supply needle has a longitudinal groove for performing equalization of pressure in the respective collecting well while the supply needle is supplying the suspension into the corresponding filtration chamber.
  • a groove can be connected to pressure equalization means of the multi-well filtration device, e.g. a pressure equalization channel, such that the pressure in the collecting well and the filtration chamber can easily be equalized.
  • a third aspect of the invention deals with a method for analyzing the solid phase of a suspension by means of an analyzing system described above.
  • the method comprises the steps of: supplying the suspension into the filtration chambers at an elevated pressure level by means of the respective supply needles thereby driving the suspension through the filter element into the collecting wells; removing the filtration plate together with the separation layer from the collecting plate; transferring the filtration plate with the filter cakes of the solid phase of the suspension contained in the respective filtration chambers to the analysis device; and analyzing the solid phase through the transparent separation layer.
  • Fig. 1 shows a top view on a multi-well filtration device according to the invention
  • Fig. 2 shows a cross-sectional view along the line A-A of the multi-well filtration device of Fig. 1 ;
  • Fig. 3 shows an exploded view of the cross-sectional view of Fig. 2 ;
  • Fig. 4 shows an expanded view of a part of the cross-sectional view of Fig. 2 , where a supply needle penetrates a septum;
  • Fig. 5 shows a two-step stair-shaped elevation for receiving a sealing mat of the multi-well filtration device from Fig. 1 ;
  • Fig. 6 shows an exploded perspective view of a transfer unit for the multi-well filtration device from Fig. 1 ;
  • Fig. 7 shows a top view on the transfer unit from Fig. 6 ;
  • Fig. 8 shows a exploded cross-section view along the line A-A of the transfer unit from Fig. 7 .
  • Fig. 1 shows a top view on a top plate 1 of a multi-well filtration device according to the present invention.
  • the top plate 1 comprises 96 needle funnels for supply 11 and 96 needle funnels for extraction 13.
  • one of the needle funnels for supply 11 is equipped with a supply needle 91 and one of the needle funnels for extraction 13 is equipped with an extraction needle 92.
  • Fig. 1 at the right hand side and at the above side of the top plate 1 the top side of a filtration plate 4 is visible.
  • the filtration plate 4 is arranged below the top plate 1 as shown in Fig. 2 and Fig. 3 .
  • the top plate 1, the needle funnels for supply 11 and the needle funnels for extraction 13 are arranged in a standardized 96 wells microplate compliant structure.
  • standardized microplate compliant structures allow the use of the multi-well filtration device in a standardized infrastructure.
  • standardized liquid handling and analysis devices can be used.
  • Fig. 2 and Fig. 3 show a cross-sectional view or an exploded cross-sectional view, respectively, of the multi-well filtration device along the line A-A of Fig. 1 .
  • the multi-well filtration device comprises a septum 2 being arranged between an upper funnel plate 3 and the top plate 1.
  • the upper funnel plate 3 is followed from top to bottom by: a first sealing mat 6; a filtration plate 4; a second sealing mat 6; a transparent separation layer 5; a third sealing mat 6; a lower funnel plate 7; a fourth sealing mat 6; and a collecting plate 8.
  • the upper funnel plate 3 has alternatingly arranged through holes for supply 33 and bridging channels 31 being interconnected by pressure equalization channels 34.
  • Each through hole for supply 33 of the upper funnel plate 3 is connected to one of the needle funnels for supply 11 of the top plate 1 via a septum opening 21 of the septum 2.
  • each bridging channel 31 of the upper funnel plate 3 is connected to one of the needle funnels for extraction 13 of the top plate 1 via a septum opening 21 of the septum 2.
  • the top plate 1 has recesses 12 around the needle funnels for supply 11 and around the needle funnels for extraction 13 on its under side and the upper funnel plate 3 has ridges 32 around the through holes for supply 33 and the bridging channels 31 on its upper side.
  • the septum 2 is pressed into the recesses 12 by the ridges 32.
  • the filtration plate 4 has alternatingly arranged through holes for extraction 42 and filtration chambers 41, wherein each filtration chamber 41 is connected to one of the through holes for supply 33 of the upper funnel plate 3.
  • Each bridging channel 31 extends through one of the through holes for extraction 42 of the filtration plate 4 projecting below the filtration plate 4.
  • the filtration plate 4 is connected to the transparent separation layer 5, being again connected to the lower funnel plate 7, wherein a sealing mat 6 is arranged between the transparent separation layer 5 and the lower funnel plate 7.
  • the lower funnel plate 7 has alternatingly arranged through holes for extraction 72 and filtrate funnels 71, wherein each filtrate funnel 71 is connected to one of the filtration chambers 41.
  • Each bridging channel 31 of the upper funnel plate 3 extends again through one of the through holes for extraction 72.
  • each filtrate funnel 71 is equipped with a filter element 73 having passages of a certain diameter.
  • the transparent separation layer 5 has holes (not shown in the figures) having a diameter larger than the diameter of the passages of the filter elements 73.
  • each of the filter elements 73 is arranged as a round metal mesh being inserted into a widened top part of the corresponding filtrate funnel 71 and being compressed with the widened top part of the corresponding filtrate funnel 71.
  • each metal mesh is preferably reversed around the filtrate funnel 71 at its lateral end section such that the metal mesh is press fitted with the lower funnel plate 7.
  • the pore size of the filter element is about 1 ⁇ m to about 2 ⁇ m.
  • the lower funnel plate 7 is connected to the collecting plate 8, wherein a sealing mat 6 is arranged in-between.
  • the collecting plate 8 has collecting wells 81 with elongated cross-sections having the form of rounded rectangles. Each of said collecting wells 81 is connected to one filtrate funnel 71 of the lower funnel plate 7 and to one bridging channel 31 of the upper funnel plate 3.
  • the bottoms of the collecting wells 81 are slightly slanted and well rounded, wherein each collecting well 81 has a deepest point 811 lying essentially straight below the bridging channel 31 being connected to said collecting well 81.
  • one of the septum openings 21 being connected to one of the filtration chambers 41 is penetrated by a supply needle 91, such that the supply needle 91 extends into said filtration chamber 41.
  • the supply needle 91 has a tapered portion for accommodating the septum opening 21 of the septum 2.
  • the supply needle 91 supplies a suspension into the filtration chamber 41 thereby creating an overpressure inside the filtration chamber 41 in order to drive the suspension through the filter element 73 into the collecting well 81.
  • the overpressure can be provided by pressure means of the supply needle 91.
  • said overpressure provision for driving the filtration has the advantage that crystal formation in the suspension is low compared to driving filtration by creating a vacuum in the collecting well 81. Therefore it is possible to get a filtrate with a comparably high concentration of solute without interfering seeds.
  • parts of the multi-well filtration device being possibly in contact with the suspension are preferably made of an isolating material, such that the cooling of the suspension being filtered at an elevated temperature is as low as possible.
  • the supply needle 91 has a longitudinal groove being connected to the according pressure equalization channel 92.
  • the pressure can be equalized between the collecting well 81 and the air pressure outside the multi-well filtration device.
  • the filter element 73 retains solids of the supplied suspension, which are not able to pass the passages. Thereby a filter cake is built on top of the transparent separation layer 5.
  • the diameter of the holes of the transparent separation layer 5 is large enough not to essentially effect the filtration and in the meantime it is small enough to be able to hold back the filter cake.
  • the septum opening 21 being connected to the according bridging channel 31 is penetrated by an extraction needle 92, such that it extends near the deepest point 811 of the bottom of the collecting well 81. Since the bottom of the collecting well 81 is slightly slanted and well rounded, the filtrate can then efficiently be extracted preventing a comparably high dead volume of filtrate in the collecting well 81.
  • the filtration plate 4 can easily be separated from the lower funnel plate 7 by means of the transparent separation layer 5.
  • the filter cake containing crystals and other solids to be analyzed is still held inside the filtration chambers 41 by the transparent separation layer 5. Without any laborious preparation steps the filter cake can be transferred into an analysis device and it can be analyzed through the transparent separation layer 5 by an appropriate analysis method, such as X-ray powder diffraction or infrared and Raman spectroscopy.
  • Fig. 5 shows a two-step stair-shaped elevation for receiving a sealing mat 6.
  • the border around one of the two openings has preferably a two-step stair-shaped elevation.
  • the sealing mat 6 is lifted and arranged on top of a sealing ridge 74 touching a guiding ridge 75.
  • the sealing mat 6 is lifted around the opening and when the two layers are connected it is compressed in said lifted area.
  • a transfer unit comprising the filtration plate 4 with the two adjacent sealing mats 6 and the separation layer 5.
  • a closing layer 5A followed by a top plate 1A are arranged on top of the upper sealing mat 6 .
  • a closing layer 5B followed by a bottom plate 1B are arranged below the separation layer 5.
  • the filtration layer 4 together with the two adjacent sealing mats 6 and the separation layer 5 can easily be separated from rest of the multi-well filtration device as described above.
  • the filtration plate 4 can then be closed as well as at its upper surface as at its bottom surface by closing layers 5A and 5B followed by a top plate 1A or a bottom plate, respectively.
  • the top plate 1A can be firmly connected to the bottom plate 1B in order to form a compact transfer unit.
  • the top plate 1A is provided with screw holes 12A
  • the filtration plate 4 is provided with screw holes 43
  • the bottom plate 1B is provided with screw holes 12B, such that the top plate 1A can be firmly connected to the bottom plate 1B by means of screws extending through said screw holes 12 A, 43 and 12B.
  • the transfer unit still comprising the filter cakes inside the filtration chambers 41 of the filtration plate 4, can then be comfortably moved, stored or transferred to the according analysis device.
  • the upper plate 1A and the bottom plate 1B are provided with through holes 11A and 11B being arranged adjacent to the filtration chambers 41 as well as the closing layers 5A and 5B are made of a transparent material, such that the filter cakes can be analyzed through the through holes 11A and 11B and the closing layers 5A and 5B by an appropriate analysis method, such as X-ray powder diffraction or infrared and Raman spectroscopy.

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (11)

  1. Dispositif de filtration multipuits pour filtrer une suspension, comprenant une plaque de filtration (4) comportant un nombre de chambres de filtration (41) et une plaque de collecte (8) avec un nombre correspondant de puits de collecte (81), dans lequel chaque chambre de filtration (41) est connectée à un puits de collecte correspondant (81) et dans lequel un élément filtrant (73) comportant des passages est placé entre chaque chambre de filtration (41) et le puits de collecte (81) correspondant, caractérisé en ce qu'une couche de séparation (5) est placée entre la plaque de filtration (4) et l'élément filtrant (73), ladite couche de séparation (5) étant transparente et comportant des trous dans une zone en contact avec l'élément filtrant, lesdits trous ayant un diamètre plus grand que le diamètre des passages de l'élément filtrant et étant adaptés pour être aptes à retenir un gâteau de filtration d'une phase solide de la suspension à l'intérieur de chaque chambre de filtration sans effectuer substantiellement la filtration.
  2. Dispositif de filtration multipuits selon la revendication 1, dans lequel chaque puits de collecte (81) en section axiale a une forme allongée et dans lequel une partie la plus profonde (811) du puits de collecte (81) est placée à une région d'extrémité longitudinale du puits de collecte.
  3. Dispositif de filtration multipuits selon l'une quelconque des revendications 1 et 2, comprenant en outre une plaque entonnoir inférieure (7) qui est placée entre la plaque de filtration (4) et la plaque de collecte (8), dans lequel la plaque entonnoir inférieure (7) a un nombre correspondant d'entonnoirs à filtrat (71) reliant chaque chambre de filtration (41) au puits de collecte (81) correspondant, et dans lequel l'élément filtrant (73) est placé au niveau d'une partie supérieure de chaque entonnoir à filtrat (71) adjacente à la couche de séparation.
  4. Dispositif de filtration multipuits selon l'une quelconque des revendications 1 à 3, comprenant en outre une plaque entonnoir supérieure (3) munie de canaux de pontage (31), dans lequel la plaque de filtration (4) comporte des trous traversants pour l'extraction (42), chaque trou traversant étant connecté à un puits de collecte (81) respectif, et dans lequel le canal de pontage (31) respectif s'étend à travers le trou traversant respectif pour l'extraction (42) dans le puits de collecte (81) respectif, de sorte que la plaque entonnoir supérieure (3) est connectée à la plaque de collecte (8) via les canaux de pontage (31).
  5. Dispositif de filtration multipuits selon l'une quelconque des revendications 1 à 4, comprenant en outre une plaque supérieure (1) munie d'entonnoirs à aiguille (11, 13) et un septum transperçable (2) ayant des ouvertures de septum correspondantes (21), dans lequel le septum (2) est placé entre la plaque supérieure (1) et respectivement la plaque entonnoir supérieure (3) ou la plaque de filtration, de sorte que les entonnoirs à aiguille (11, 13) sont connectés à une chambre de filtration correspondante (41) et que les ouvertures de septum (21) sont adjacentes aux entonnoirs à aiguille (11, 13).
  6. Dispositif de filtration multipuits selon la revendication 5, dans lequel la plaque supérieure (1) a un évidement (12) autour de chaque entonnoir à aiguille (11, 13) du côté faisant face au septum (2) et dans lequel la plaque entonnoir supérieure (3) ou la plaque de filtration, respectivement, a une arête correspondante (32) sur le côté faisant face au septum, de sorte que lors de l'assemblage, le septum (2) est pressé dans l'évidement (12) par l'arête (32).
  7. Dispositif de filtration multipuits selon l'une quelconque des revendications 1 à 6, comprenant en outre des canaux d'équilibrage de pression (34) pour égaliser la pression dans chaque puits de collecte (81).
  8. Dispositif de filtration multipuits selon l'une quelconque des revendications 1 à 7, dans lequel un mat d'étanchéité (6) est placé entre deux plaques adjacentes, ledit mat d'étanchéité (6) ayant des trous situés en correspondance avec des ouvertures adjacentes des deux plaques, et l'une desdites deux plaques comportant une arête d'étanchéité (74) du côté faisant face au mat d'étanchéité (6) et étant ainsi apte à recevoir le bord du trou au sommet de l'arête d'étanchéité (74).
  9. Système pour analyser la phase solide d'une suspension, comprenant un nombre d'aiguilles d'alimentation (91) pour introduire la suspension dans un nombre correspondant de chambres de filtration (41) à un niveau de pression élevé, un nombre correspondant d'aiguilles d'extraction (92) pour extraire un filtrat d'un nombre correspondant de puits de collecte (81), et un dispositif d'analyse pour analyser une phase solide de la suspension filtrée par un dispositif de filtration multipuits, caractérisé par un dispositif de filtration multipuits selon l'une quelconque des revendications 1 à 8, et en ce que le gâteau de filtration respectif de la phase solide de la suspension est analysé par le dispositif d'analyse à travers la couche de séparation (5) pendant que le gâteau de filtration est situé dans la chambre de filtration respective (41) de la plaque de filtration (4) du dispositif de filtration multipuits.
  10. Système selon la revendication 9, dans lequel chaque aiguille d'alimentation (91) a une rainure longitudinale pour réaliser un équilibrage de pression dans le puits de collecte respectif pendant que l'aiguille d'alimentation (91) introduit la suspension dans la chambre de filtration (41) correspondante.
  11. Procédé d'analyse de la phase solide d'une suspension filtrée, comprenant les étapes suivantes :
    introduire une suspension dans un nombre de chambres de filtration (41),
    entraîner la suspension à travers un élément filtrant (73) dans un nombre correspondant de puits de collecte (81), et
    analyser une phase solide de la suspension retenue par l'élément filtrant (73),
    caractérisé par les étapes suivantes :
    utiliser un système selon la revendication 8 ou 9, dans lequel ladite suspension est envoyée dans la chambre de filtration respective (41) de la plaque de filtration (4) à un niveau de pression élevé au moyen d'une aiguille d'alimentation respective (91),
    après la filtration, retirer la plaque de filtration (4) en même temps que la couche de séparation (5) de la plaque de collecte (8), transférer la plaque de filtration (4) avec un gâteau de filtration de la phase solide de la suspension contenue dans la chambre de filtration respective vers le dispositif d'analyse, et
    analyser le gâteau de filtration respectif à travers la couche de séparation (5).
EP20070107643 2006-05-12 2007-05-07 Dispositif filtrant multipuits Active EP1854542B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP20070107643 EP1854542B1 (fr) 2006-05-12 2007-05-07 Dispositif filtrant multipuits

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06405198A EP1854540A1 (fr) 2006-05-12 2006-05-12 Dispositif de filtration à puits multiples
EP20070107643 EP1854542B1 (fr) 2006-05-12 2007-05-07 Dispositif filtrant multipuits

Publications (2)

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EP1854542A1 EP1854542A1 (fr) 2007-11-14
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3655128B1 (fr) * 2017-09-29 2023-08-30 Sartorius Stedim Biotech GmbH Dispositif de filtration, procédé d'assemblage d'un dispositif de filtration modulaire, et procédé permettant de caractériser un milieu filtrant et/ou un milieu à filtrer

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1854540A1 (fr) * 2006-05-12 2007-11-14 F. Hoffmann-la Roche AG Dispositif de filtration à puits multiples
IN2012DN01702A (fr) * 2009-07-31 2015-06-05 Stafford Simon
DE102010011485A1 (de) 2010-03-16 2011-09-22 Sartorius Stedim Biotech Gmbh Mehrfachlochplatte mit Filtermedium und ihre Verwendung

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US5141719A (en) * 1990-07-18 1992-08-25 Bio-Rad Laboratories, Inc. Multi-sample filtration plate assembly
US5972694A (en) * 1997-02-11 1999-10-26 Mathus; Gregory Multi-well plate
US6159368A (en) * 1998-10-29 2000-12-12 The Perkin-Elmer Corporation Multi-well microfiltration apparatus
WO2001019520A1 (fr) * 1999-09-13 2001-03-22 Millipore Corporation Carte haute densite, de preparation d'echantillons, coulee sur place
US20030143124A1 (en) * 2002-01-31 2003-07-31 Roberts Roger Q. Unidirectional flow control sealing matt
EP1560651A1 (fr) * 2002-11-12 2005-08-10 Millipore Corporation Dispositif de regulation de l'evaporation pour plaques a multipuits
CA2467131C (fr) * 2003-05-13 2013-12-10 Becton, Dickinson & Company Appareil et methode de traitement d'echantillons biologiques et chimiques

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3655128B1 (fr) * 2017-09-29 2023-08-30 Sartorius Stedim Biotech GmbH Dispositif de filtration, procédé d'assemblage d'un dispositif de filtration modulaire, et procédé permettant de caractériser un milieu filtrant et/ou un milieu à filtrer

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