EP1639622A2 - Source d'ions de desorption laser - Google Patents

Source d'ions de desorption laser

Info

Publication number
EP1639622A2
EP1639622A2 EP04754443A EP04754443A EP1639622A2 EP 1639622 A2 EP1639622 A2 EP 1639622A2 EP 04754443 A EP04754443 A EP 04754443A EP 04754443 A EP04754443 A EP 04754443A EP 1639622 A2 EP1639622 A2 EP 1639622A2
Authority
EP
European Patent Office
Prior art keywords
sample
ions
ion
gas phase
desorption
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP04754443A
Other languages
German (de)
English (en)
Other versions
EP1639622B1 (fr
EP1639622A4 (fr
Inventor
Ross C. Willoughby
Edward W. Sheehan
Craig M. c/o Analytica of Branford WHITEHOUSE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP1639622A2 publication Critical patent/EP1639622A2/fr
Publication of EP1639622A4 publication Critical patent/EP1639622A4/fr
Application granted granted Critical
Publication of EP1639622B1 publication Critical patent/EP1639622B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
    • H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/0027—Methods for using particle spectrometers
    • H01J49/0031—Step by step routines describing the use of the apparatus
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/06—Electron- or ion-optical arrangements

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Engineering & Computer Science (AREA)
  • Plasma & Fusion (AREA)
  • Electron Tubes For Measurement (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Electron Sources, Ion Sources (AREA)

Abstract

L'invention concerne des procédés d'ionisation à la pression atmosphérique, à pression intermédiaire et de désorption-ionisation laser sous vide et des sources d'ions qui sont conçus pour augmenter le rendement de l'ionisation et le rendement de transmissions d'ions à une masse afin de charger l'analyseur ou l'analyseur de mobilité des ions. Un champ électrique est appliqué sur la région d'un échantillon cible afin d'accumuler les ions générés par une sources d'ions locale sur un échantillon en phase solide ou liquide avant d'appliquer une impulsion de désorption laser. Le champ électrique est modifié juste avant ou pendant l'impulsion de désorption laser afin de stimuler la désorption de l'espèce chargée et d'améliorer le rendement d'ionisation de l'espèce de l'échantillon désorbé. Après un certain délai, le champ électrique peut être de nouveau modifié afin d'optimiser la focalisation et la transmission d'ions à un spectromètre de masse ou à un analyseur de mobilité des ions. L'espèce chargée peut être également ajoutée à la région du panache de l'échantillon désorbé afin de stimuler les réactions ions-molécules entre les ions ajoutés et l'espèce de l'échantillon neutre désorbé, renforçant ainsi le rendement d'ionisation de l'échantillon désorbé et/ou créant une espèce d'ion souhaitée. Les cycles de modifications du champ électrique sont répétés dans une séquence chronométrée à raison d'une ou plusieurs impulsion de désorption laser par cycle de modification du champ électrique. Des modes de réalisation selon l'invention font intervenir des procédés et des dispositifs d'ionisation à la pression atmosphérique, à pression intermédiaire et de désorption-ionisation laser sous pression source afin d'augmenter la flexibilité analytique et d'améliorer la sensibilité d'analyse par spectrométrie de masse.
EP04754443.2A 2003-06-07 2004-06-07 Source d'ions de desorption laser Active EP1639622B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47657603P 2003-06-07 2003-06-07
PCT/US2004/017832 WO2004112074A2 (fr) 2003-06-07 2004-06-07 Source d'ions de desorption laser

Publications (3)

Publication Number Publication Date
EP1639622A2 true EP1639622A2 (fr) 2006-03-29
EP1639622A4 EP1639622A4 (fr) 2007-11-21
EP1639622B1 EP1639622B1 (fr) 2016-11-16

Family

ID=33551616

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04754443.2A Active EP1639622B1 (fr) 2003-06-07 2004-06-07 Source d'ions de desorption laser

Country Status (3)

Country Link
EP (1) EP1639622B1 (fr)
CA (1) CA2527886C (fr)
WO (1) WO2004112074A2 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG158737A1 (en) * 2002-10-29 2010-02-26 Target Discovery Inc Method for increasing ionization efficiency in mass spectroscopy
DE102004002729B4 (de) 2004-01-20 2008-11-27 Bruker Daltonik Gmbh Ionisierung desorbierter Analytmoleküle bei Atmosphärendruck
US7737395B2 (en) 2006-09-20 2010-06-15 Agilent Technologies, Inc. Apparatuses, methods and compositions for ionization of samples and mass calibrants
CZ304648B6 (cs) * 2007-12-03 2014-08-20 Masarykova Univerzita Způsob přípravy vzorků pro atomové spektrometrické techniky
US8704169B2 (en) 2011-10-11 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Direct impact ionization (DII) mass spectrometry
WO2016142685A1 (fr) 2015-03-06 2016-09-15 Micromass Uk Limited Surface de collision pour ionisation améliorée
CN108700590B (zh) 2015-03-06 2021-03-02 英国质谱公司 细胞群体分析
CN107646089B (zh) 2015-03-06 2020-12-08 英国质谱公司 光谱分析
KR102092047B1 (ko) 2015-03-06 2020-03-24 마이크로매스 유케이 리미티드 생체내 내시경 조직 식별 도구
US11289320B2 (en) 2015-03-06 2022-03-29 Micromass Uk Limited Tissue analysis by mass spectrometry or ion mobility spectrometry
JP6753862B2 (ja) 2015-03-06 2020-09-09 マイクロマス ユーケー リミテッド 気体サンプルの改良されたイオン化
EP3265820B1 (fr) 2015-03-06 2023-12-13 Micromass UK Limited Analyse spectrométrique de microbes
US10026599B2 (en) 2015-03-06 2018-07-17 Micromass Uk Limited Rapid evaporative ionisation mass spectrometry (“REIMS”) and desorption electrospray ionisation mass spectrometry (“DESI-MS”) analysis of swabs and biopsy samples
EP3726562B1 (fr) 2015-03-06 2023-12-20 Micromass UK Limited Plateforme d'imagerie de spectrométrie de masse par ionisation ambiante pour la cartographie directe à partir de tissu en vrac
GB201517195D0 (en) 2015-09-29 2015-11-11 Micromass Ltd Capacitively coupled reims technique and optically transparent counter electrode
US11454611B2 (en) 2016-04-14 2022-09-27 Micromass Uk Limited Spectrometric analysis of plants
EP3909067A1 (fr) * 2019-01-11 2021-11-17 Helmholtz-Zentrum Potsdam - Deutsches GeoForschungsZentrum GFZ Stiftung des Öffentlichen Rechts des Lands Brandenburg Source d'ions comprenant un échantillon structuré pour ionisation améliorée
CN111912895A (zh) * 2019-05-09 2020-11-10 岛津分析技术研发（上海）有限公司 真空下的质谱成像装置及方法
CA3203069A1 (fr) * 2020-12-21 2022-06-30 Ankur CHAUDHURI Appareil de source d'ions laser compact et procede correspondant

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5625184A (en) * 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
US5663561A (en) * 1995-03-28 1997-09-02 Bruker-Franzen Analytik Gmbh Method for the ionization of heavy molecules at atmospheric pressure
GB2348049A (en) * 1999-03-17 2000-09-20 Bruker Daltonik Gmbh An ion source for mass spectrometry

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3125335A1 (de) * 1981-06-27 1983-01-13 Alfred Prof. Dr. 4400 Münster Benninghoven Verfahren zur analyse von gasen und fluessigkeiten
US5965884A (en) 1998-06-04 1999-10-12 The Regents Of The University Of California Atmospheric pressure matrix assisted laser desorption
US6744041B2 (en) 2000-06-09 2004-06-01 Edward W Sheehan Apparatus and method for focusing ions and charged particles at atmospheric pressure
US6683301B2 (en) 2001-01-29 2004-01-27 Analytica Of Branford, Inc. Charged particle trapping in near-surface potential wells
EP1402561A4 (fr) 2001-05-25 2007-06-06 Analytica Of Branford Inc Source d'ions maldi atmospherique et sous depression
US6707036B2 (en) 2002-03-21 2004-03-16 Thermo Finnigan Llc Ionization apparatus and method for mass spectrometer system
US6707039B1 (en) 2002-09-19 2004-03-16 Agilent Technologies, Inc. AP-MALDI target illumination device and method for using an AP-MALDI target illumination device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5663561A (en) * 1995-03-28 1997-09-02 Bruker-Franzen Analytik Gmbh Method for the ionization of heavy molecules at atmospheric pressure
US5625184A (en) * 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
GB2348049A (en) * 1999-03-17 2000-09-20 Bruker Daltonik Gmbh An ion source for mass spectrometry

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2004112074A2 *

Also Published As

Publication number Publication date
WO2004112074A2 (fr) 2004-12-23
EP1639622B1 (fr) 2016-11-16
WO2004112074A3 (fr) 2005-10-06
CA2527886A1 (fr) 2004-12-23
CA2527886C (fr) 2014-01-14
EP1639622A4 (fr) 2007-11-21

Similar Documents

Publication Publication Date Title
US7087898B2 (en) Laser desorption ion source
US7375319B1 (en) Laser desorption ion source
US6204500B1 (en) Mass spectrometry from surfaces
CA2527886C (fr) Source d'ions de desorption laser
US6683301B2 (en) Charged particle trapping in near-surface potential wells
CA2448335C (fr) Source d'ions maldi atmospherique et sous depression
US7858926B1 (en) Mass spectrometry with segmented RF multiple ion guides in various pressure regions
US7189967B1 (en) Mass spectrometry with multipole ion guides
CA2567466C (fr) Surfaces rf et guides d'ions rf
US6753523B1 (en) Mass spectrometry with multipole ion guides
US6987264B1 (en) Mass spectrometry with multipole ion guides
US7109480B2 (en) Ion source and methods for MALDI mass spectrometry
EP1057209B1 (fr) Spectrometrie de masse a guide d'ions multipolaire
KR20050056937A (ko) 탠덤 비행시간 질량 분석기 및 그 사용방법
WO2002083275A9 (fr) Procede et systeme de spectroscopie de masse
CA2641940C (fr) Spectrometrie de masse a multiples guides ioniques rf segmentes en plusieurs zones de pression

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20051229

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL HR LT LV MK

DAX Request for extension of the european patent (deleted)
RBV Designated contracting states (corrected)

Designated state(s): DE FR GB

A4 Supplementary search report drawn up and despatched

Effective date: 20071023

REG Reference to a national code

Ref country code: DE

Ref legal event code: R079

Ref document number: 602004050308

Country of ref document: DE

Free format text: PREVIOUS MAIN CLASS: H01J0049160000

Ipc: H01J0049000000

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20160603

RIC1 Information provided on ipc code assigned before grant

Ipc: H01J 49/00 20060101AFI20160520BHEP

Ipc: H01J 49/16 20060101ALI20160520BHEP

Ipc: H01J 49/06 20060101ALI20160520BHEP

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): DE FR GB

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602004050308

Country of ref document: DE

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 602004050308

Country of ref document: DE

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 602004050308

Country of ref document: DE

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20170817

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

Effective date: 20180228

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20170630

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230601

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20230418

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20230420

Year of fee payment: 20