CA2527886C - Source d'ions de desorption laser - Google Patents
Source d'ions de desorption laser Download PDFInfo
- Publication number
- CA2527886C CA2527886C CA2527886A CA2527886A CA2527886C CA 2527886 C CA2527886 C CA 2527886C CA 2527886 A CA2527886 A CA 2527886A CA 2527886 A CA2527886 A CA 2527886A CA 2527886 C CA2527886 C CA 2527886C
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/10—Ion sources; Ion guns
- H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
- H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
- H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0031—Step by step routines describing the use of the apparatus
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/06—Electron- or ion-optical arrangements
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Electron Tubes For Measurement (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Sources, Ion Sources (AREA)
Abstract
L'invention concerne des procédés d'ionisation à la pression atmosphérique, à pression intermédiaire et de désorption-ionisation laser sous vide et des sources d'ions qui sont conçus pour augmenter le rendement de l'ionisation et le rendement de transmissions d'ions à une masse afin de charger l'analyseur ou l'analyseur de mobilité des ions. Un champ électrique est appliqué sur la région d'un échantillon cible afin d'accumuler les ions générés par une sources d'ions locale sur un échantillon en phase solide ou liquide avant d'appliquer une impulsion de désorption laser. Le champ électrique est modifié juste avant ou pendant l'impulsion de désorption laser afin de stimuler la désorption de l'espèce chargée et d'améliorer le rendement d'ionisation de l'espèce de l'échantillon désorbé. Après un certain délai, le champ électrique peut être de nouveau modifié afin d'optimiser la focalisation et la transmission d'ions à un spectromètre de masse ou à un analyseur de mobilité des ions. L'espèce chargée peut être également ajoutée à la région du panache de l'échantillon désorbé afin de stimuler les réactions ions-molécules entre les ions ajoutés et l'espèce de l'échantillon neutre désorbé, renforçant ainsi le rendement d'ionisation de l'échantillon désorbé et/ou créant une espèce d'ion souhaitée. Les cycles de modifications du champ électrique sont répétés dans une séquence chronométrée à raison d'une ou plusieurs impulsion de désorption laser par cycle de modification du champ électrique. Des modes de réalisation selon l'invention font intervenir des procédés et des dispositifs d'ionisation à la pression atmosphérique, à pression intermédiaire et de désorption-ionisation laser sous pression source afin d'augmenter la flexibilité analytique et d'améliorer la sensibilité d'analyse par spectrométrie de masse.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US47657603P | 2003-06-07 | 2003-06-07 | |
| US60/476,576 | 2003-06-07 | ||
| PCT/US2004/017832 WO2004112074A2 (fr) | 2003-06-07 | 2004-06-07 | Source d'ions de desorption laser |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2527886A1 CA2527886A1 (fr) | 2004-12-23 |
| CA2527886C true CA2527886C (fr) | 2014-01-14 |
Family
ID=33551616
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2527886A Expired - Lifetime CA2527886C (fr) | 2003-06-07 | 2004-06-07 | Source d'ions de desorption laser |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1639622B1 (fr) |
| CA (1) | CA2527886C (fr) |
| WO (1) | WO2004112074A2 (fr) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2498878C (fr) * | 2002-10-29 | 2013-01-08 | Target Discovery, Inc. | Procede permettant d'accroitre l'efficacite d'ionisation en spectroscopie de masse |
| DE102004002729B4 (de) * | 2004-01-20 | 2008-11-27 | Bruker Daltonik Gmbh | Ionisierung desorbierter Analytmoleküle bei Atmosphärendruck |
| US7737395B2 (en) * | 2006-09-20 | 2010-06-15 | Agilent Technologies, Inc. | Apparatuses, methods and compositions for ionization of samples and mass calibrants |
| CZ304648B6 (cs) * | 2007-12-03 | 2014-08-20 | Masarykova Univerzita | Způsob přípravy vzorků pro atomové spektrometrické techniky |
| US8704169B2 (en) | 2011-10-11 | 2014-04-22 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Direct impact ionization (DII) mass spectrometry |
| WO2016142691A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Analyse par spectrométrie de masse par ionisation par évaporation rapide (« reims ») et spectrométrie de masse par ionisation par electronébulisation par désorption (« desi-ms ») d'écouvillons et d'échantillons de biopsie |
| WO2016142685A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Surface de collision pour ionisation améliorée |
| US11282688B2 (en) | 2015-03-06 | 2022-03-22 | Micromass Uk Limited | Spectrometric analysis of microbes |
| US11270876B2 (en) | 2015-03-06 | 2022-03-08 | Micromass Uk Limited | Ionisation of gaseous samples |
| KR102092047B1 (ko) | 2015-03-06 | 2020-03-24 | 마이크로매스 유케이 리미티드 | 생체내 내시경 조직 식별 도구 |
| US11289320B2 (en) | 2015-03-06 | 2022-03-29 | Micromass Uk Limited | Tissue analysis by mass spectrometry or ion mobility spectrometry |
| EP3726562B1 (fr) | 2015-03-06 | 2023-12-20 | Micromass UK Limited | Plateforme d'imagerie de spectrométrie de masse par ionisation ambiante pour la cartographie directe à partir de tissu en vrac |
| CN108700590B (zh) | 2015-03-06 | 2021-03-02 | 英国质谱公司 | 细胞群体分析 |
| WO2016142692A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Analyse spectrométrique |
| GB201517195D0 (en) | 2015-09-29 | 2015-11-11 | Micromass Ltd | Capacitively coupled reims technique and optically transparent counter electrode |
| EP3443354A1 (fr) | 2016-04-14 | 2019-02-20 | Micromass UK Limited | Analyse spectrométrique de plantes |
| WO2020144321A1 (fr) * | 2019-01-11 | 2020-07-16 | HELMHOLTZ-ZENTRUM POTSDAM - Deutsches-Geoforschungszentrum GFZ Stiftung des Öffentlichen Rechts des Landes Brandenburg | Source d'ions comprenant un échantillon structuré pour ionisation améliorée |
| CN111912895A (zh) * | 2019-05-09 | 2020-11-10 | 岛津分析技术研发（上海）有限公司 | 真空下的质谱成像装置及方法 |
| WO2022133593A1 (fr) * | 2020-12-21 | 2022-06-30 | Atomic Energy Of Canada Limited / Énergie Atomique Du Canada Limitée | Appareil de source d'ions laser compact et procédé correspondant |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3125335A1 (de) * | 1981-06-27 | 1983-01-13 | Alfred Prof. Dr. 4400 Münster Benninghoven | Verfahren zur analyse von gasen und fluessigkeiten |
| DE19608963C2 (de) * | 1995-03-28 | 2001-03-22 | Bruker Daltonik Gmbh | Verfahren zur Ionisierung schwerer Moleküle bei Atmosphärendruck |
| US5625184A (en) * | 1995-05-19 | 1997-04-29 | Perseptive Biosystems, Inc. | Time-of-flight mass spectrometry analysis of biomolecules |
| US5965884A (en) | 1998-06-04 | 1999-10-12 | The Regents Of The University Of California | Atmospheric pressure matrix assisted laser desorption |
| DE19911801C1 (de) * | 1999-03-17 | 2001-01-11 | Bruker Daltonik Gmbh | Verfahren und Vorrichtung zur matrixunterstützten Laserdesorptions-Ionisierung von Substanzen |
| US6744041B2 (en) | 2000-06-09 | 2004-06-01 | Edward W Sheehan | Apparatus and method for focusing ions and charged particles at atmospheric pressure |
| US6683301B2 (en) | 2001-01-29 | 2004-01-27 | Analytica Of Branford, Inc. | Charged particle trapping in near-surface potential wells |
| CA2448335C (fr) * | 2001-05-25 | 2010-01-26 | Analytica Of Branford, Inc. | Source d'ions maldi atmospherique et sous depression |
| US6707036B2 (en) | 2002-03-21 | 2004-03-16 | Thermo Finnigan Llc | Ionization apparatus and method for mass spectrometer system |
| US6707039B1 (en) | 2002-09-19 | 2004-03-16 | Agilent Technologies, Inc. | AP-MALDI target illumination device and method for using an AP-MALDI target illumination device |
-
2004
- 2004-06-07 EP EP04754443.2A patent/EP1639622B1/fr not_active Expired - Lifetime
- 2004-06-07 WO PCT/US2004/017832 patent/WO2004112074A2/fr active Application Filing
- 2004-06-07 CA CA2527886A patent/CA2527886C/fr not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1639622B1 (fr) | 2016-11-16 |
| WO2004112074A2 (fr) | 2004-12-23 |
| EP1639622A4 (fr) | 2007-11-21 |
| WO2004112074A3 (fr) | 2005-10-06 |
| CA2527886A1 (fr) | 2004-12-23 |
| EP1639622A2 (fr) | 2006-03-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |