CA2527886C - Source d'ions de desorption laser - Google Patents
Source d'ions de desorption laser Download PDFInfo
- Publication number
- CA2527886C CA2527886C CA2527886A CA2527886A CA2527886C CA 2527886 C CA2527886 C CA 2527886C CA 2527886 A CA2527886 A CA 2527886A CA 2527886 A CA2527886 A CA 2527886A CA 2527886 C CA2527886 C CA 2527886C
- Authority
- CA
- Canada
- Prior art keywords
- sample
- ions
- ion
- gas phase
- charge
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000003795 desorption Methods 0.000 title abstract description 117
- 150000002500 ions Chemical class 0.000 claims abstract description 448
- 230000005684 electric field Effects 0.000 claims abstract description 55
- 230000007935 neutral effect Effects 0.000 claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 26
- 239000003153 chemical reaction reagent Substances 0.000 claims description 54
- 238000004458 analytical method Methods 0.000 claims description 29
- 230000001360 synchronised effect Effects 0.000 claims description 10
- 239000013626 chemical specie Substances 0.000 claims description 9
- 238000012546 transfer Methods 0.000 claims description 3
- 238000001698 laser desorption ionisation Methods 0.000 abstract description 35
- 230000005540 biological transmission Effects 0.000 abstract description 18
- 238000006243 chemical reaction Methods 0.000 abstract description 8
- 230000035945 sensitivity Effects 0.000 abstract description 6
- 230000008859 change Effects 0.000 abstract description 5
- 230000001351 cycling effect Effects 0.000 abstract description 3
- 238000004949 mass spectrometry Methods 0.000 abstract description 3
- 239000007791 liquid phase Substances 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 278
- 239000007789 gas Substances 0.000 description 137
- 238000007600 charging Methods 0.000 description 114
- 238000010586 diagram Methods 0.000 description 38
- 241000894007 species Species 0.000 description 28
- 239000007788 liquid Substances 0.000 description 23
- 238000000605 extraction Methods 0.000 description 21
- 238000009825 accumulation Methods 0.000 description 18
- 239000011159 matrix material Substances 0.000 description 16
- 239000000835 fiber Substances 0.000 description 12
- 230000033001 locomotion Effects 0.000 description 12
- 238000003384 imaging method Methods 0.000 description 10
- 239000012491 analyte Substances 0.000 description 9
- 238000000816 matrix-assisted laser desorption--ionisation Methods 0.000 description 9
- 230000005405 multipole Effects 0.000 description 9
- 238000002663 nebulization Methods 0.000 description 9
- 239000000203 mixture Substances 0.000 description 8
- 239000007921 spray Substances 0.000 description 8
- 210000001519 tissue Anatomy 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 7
- 238000001035 drying Methods 0.000 description 7
- 238000001704 evaporation Methods 0.000 description 7
- 230000032258 transport Effects 0.000 description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 238000011109 contamination Methods 0.000 description 6
- 230000003287 optical effect Effects 0.000 description 6
- 238000005457 optimization Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000001133 acceleration Effects 0.000 description 5
- 238000000065 atmospheric pressure chemical ionisation Methods 0.000 description 5
- 238000004811 liquid chromatography Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000012634 optical imaging Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 239000013078 crystal Substances 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 239000012212 insulator Substances 0.000 description 4
- 238000000752 ionisation method Methods 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000000451 chemical ionisation Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000010304 firing Methods 0.000 description 3
- 230000037427 ion transport Effects 0.000 description 3
- 238000005040 ion trap Methods 0.000 description 3
- 238000000960 laser cooling Methods 0.000 description 3
- 229920002521 macromolecule Polymers 0.000 description 3
- 238000001819 mass spectrum Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000004252 FT/ICR mass spectrometry Methods 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 239000003990 capacitor Substances 0.000 description 2
- 239000012159 carrier gas Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 239000000975 dye Substances 0.000 description 2
- 238000000132 electrospray ionisation Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000010574 gas phase reaction Methods 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 238000010884 ion-beam technique Methods 0.000 description 2
- 239000006199 nebulizer Substances 0.000 description 2
- 238000006386 neutralization reaction Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000010408 sweeping Methods 0.000 description 2
- 239000006200 vaporizer Substances 0.000 description 2
- 238000013022 venting Methods 0.000 description 2
- 230000004304 visual acuity Effects 0.000 description 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 241000102542 Kara Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 230000003190 augmentative effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 238000000738 capillary electrophoresis-mass spectrometry Methods 0.000 description 1
- 238000003981 capillary liquid chromatography Methods 0.000 description 1
- 238000012511 carbohydrate analysis Methods 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 239000004020 conductor Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000013016 damping Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003989 dielectric material Substances 0.000 description 1
- 230000005264 electron capture Effects 0.000 description 1
- 238000005421 electrostatic potential Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000005596 ionic collisions Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 150000002605 large molecules Chemical class 0.000 description 1
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 1
- 238000001840 matrix-assisted laser desorption--ionisation time-of-flight mass spectrometry Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- -1 protonated methanol ions Chemical class 0.000 description 1
- 239000012495 reaction gas Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- PCMORTLOPMLEFB-ONEGZZNKSA-N sinapic acid Chemical compound COC1=CC(\C=C\C(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-ONEGZZNKSA-N 0.000 description 1
- PCMORTLOPMLEFB-UHFFFAOYSA-N sinapinic acid Natural products COC1=CC(C=CC(O)=O)=CC(OC)=C1O PCMORTLOPMLEFB-UHFFFAOYSA-N 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
Classifications
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/10—Ion sources; Ion guns
- H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
- H01J49/161—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission using photoionisation, e.g. by laser
- H01J49/164—Laser desorption/ionisation, e.g. matrix-assisted laser desorption/ionisation [MALDI]
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/0027—Methods for using particle spectrometers
- H01J49/0031—Step by step routines describing the use of the apparatus
-
- H—ELECTRICITY
- H01—ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometers or separator tubes
- H01J49/02—Details
- H01J49/06—Electron- or ion-optical arrangements
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Plasma & Fusion (AREA)
- Electron Tubes For Measurement (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
- Electron Sources, Ion Sources (AREA)
Abstract
L'invention concerne des procédés d'ionisation à la pression atmosphérique, à pression intermédiaire et de désorption-ionisation laser sous vide et des sources d'ions qui sont conçus pour augmenter le rendement de l'ionisation et le rendement de transmissions d'ions à une masse afin de charger l'analyseur ou l'analyseur de mobilité des ions. Un champ électrique est appliqué sur la région d'un échantillon cible afin d'accumuler les ions générés par une sources d'ions locale sur un échantillon en phase solide ou liquide avant d'appliquer une impulsion de désorption laser. Le champ électrique est modifié juste avant ou pendant l'impulsion de désorption laser afin de stimuler la désorption de l'espèce chargée et d'améliorer le rendement d'ionisation de l'espèce de l'échantillon désorbé. Après un certain délai, le champ électrique peut être de nouveau modifié afin d'optimiser la focalisation et la transmission d'ions à un spectromètre de masse ou à un analyseur de mobilité des ions. L'espèce chargée peut être également ajoutée à la région du panache de l'échantillon désorbé afin de stimuler les réactions ions-molécules entre les ions ajoutés et l'espèce de l'échantillon neutre désorbé, renforçant ainsi le rendement d'ionisation de l'échantillon désorbé et/ou créant une espèce d'ion souhaitée. Les cycles de modifications du champ électrique sont répétés dans une séquence chronométrée à raison d'une ou plusieurs impulsion de désorption laser par cycle de modification du champ électrique. Des modes de réalisation selon l'invention font intervenir des procédés et des dispositifs d'ionisation à la pression atmosphérique, à pression intermédiaire et de désorption-ionisation laser sous pression source afin d'augmenter la flexibilité analytique et d'améliorer la sensibilité d'analyse par spectrométrie de masse.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US47657603P | 2003-06-07 | 2003-06-07 | |
US60/476,576 | 2003-06-07 | ||
PCT/US2004/017832 WO2004112074A2 (fr) | 2003-06-07 | 2004-06-07 | Source d'ions de desorption laser |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2527886A1 CA2527886A1 (fr) | 2004-12-23 |
CA2527886C true CA2527886C (fr) | 2014-01-14 |
Family
ID=33551616
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2527886A Expired - Lifetime CA2527886C (fr) | 2003-06-07 | 2004-06-07 | Source d'ions de desorption laser |
Country Status (3)
Country | Link |
---|---|
EP (1) | EP1639622B1 (fr) |
CA (1) | CA2527886C (fr) |
WO (1) | WO2004112074A2 (fr) |
Families Citing this family (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004088271A2 (fr) * | 2002-10-29 | 2004-10-14 | Target Discovery, Inc. | Procede permettant d'accroitre l'efficacite d'ionisation en spectroscopie de masse |
DE102004002729B4 (de) * | 2004-01-20 | 2008-11-27 | Bruker Daltonik Gmbh | Ionisierung desorbierter Analytmoleküle bei Atmosphärendruck |
US7737395B2 (en) * | 2006-09-20 | 2010-06-15 | Agilent Technologies, Inc. | Apparatuses, methods and compositions for ionization of samples and mass calibrants |
CZ304648B6 (cs) * | 2007-12-03 | 2014-08-20 | Masarykova Univerzita | Způsob přípravy vzorků pro atomové spektrometrické techniky |
US8704169B2 (en) | 2011-10-11 | 2014-04-22 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Direct impact ionization (DII) mass spectrometry |
WO2016142674A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Analyse de population de cellules |
CN107530065A (zh) | 2015-03-06 | 2018-01-02 | 英国质谱公司 | 活体内内窥镜下组织识别工具 |
CN107530064B (zh) | 2015-03-06 | 2021-07-30 | 英国质谱公司 | 气态样品的改进电离 |
US11342170B2 (en) | 2015-03-06 | 2022-05-24 | Micromass Uk Limited | Collision surface for improved ionisation |
WO2016142681A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Analyse spectrométrique de microbes |
EP3265817B1 (fr) * | 2015-03-06 | 2020-08-12 | Micromass UK Limited | Analyse par spectrométrie de masse par ionisation par évaporation rapide (« reims ») et spectrométrie de masse par ionisation par electronébulisation par désorption (« desi-ms ») d'écouvillons et d'échantillons de biopsie |
CN107646089B (zh) | 2015-03-06 | 2020-12-08 | 英国质谱公司 | 光谱分析 |
WO2016142689A1 (fr) | 2015-03-06 | 2016-09-15 | Micromass Uk Limited | Analyse tissulaire par spectrométrie de masse ou par spectrométrie de mobilité ionique |
EP3726562B1 (fr) | 2015-03-06 | 2023-12-20 | Micromass UK Limited | Plateforme d'imagerie de spectrométrie de masse par ionisation ambiante pour la cartographie directe à partir de tissu en vrac |
GB201517195D0 (en) | 2015-09-29 | 2015-11-11 | Micromass Ltd | Capacitively coupled reims technique and optically transparent counter electrode |
WO2017178833A1 (fr) | 2016-04-14 | 2017-10-19 | Micromass Uk Limited | Analyse spectrométrique de plantes |
US20220102131A1 (en) * | 2019-01-11 | 2022-03-31 | Helmholtz-Zentrum Potsdam - Deutsches Geoforschungszentrum GFZ Stiftung des Offentlichen Rechts des | Ion source including structured sample for ionization |
CN111912895A (zh) * | 2019-05-09 | 2020-11-10 | 岛津分析技术研发(上海)有限公司 | 真空下的质谱成像装置及方法 |
US20240079226A1 (en) * | 2020-12-21 | 2024-03-07 | Atomic Energy Of Canada Limited / Énergie Atomique Du Canada Limitée | Compact laser ion source apparatus and method |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3125335A1 (de) * | 1981-06-27 | 1983-01-13 | Alfred Prof. Dr. 4400 Münster Benninghoven | Verfahren zur analyse von gasen und fluessigkeiten |
DE19608963C2 (de) * | 1995-03-28 | 2001-03-22 | Bruker Daltonik Gmbh | Verfahren zur Ionisierung schwerer Moleküle bei Atmosphärendruck |
US5625184A (en) | 1995-05-19 | 1997-04-29 | Perseptive Biosystems, Inc. | Time-of-flight mass spectrometry analysis of biomolecules |
US5965884A (en) | 1998-06-04 | 1999-10-12 | The Regents Of The University Of California | Atmospheric pressure matrix assisted laser desorption |
DE19911801C1 (de) * | 1999-03-17 | 2001-01-11 | Bruker Daltonik Gmbh | Verfahren und Vorrichtung zur matrixunterstützten Laserdesorptions-Ionisierung von Substanzen |
US6744041B2 (en) | 2000-06-09 | 2004-06-01 | Edward W Sheehan | Apparatus and method for focusing ions and charged particles at atmospheric pressure |
US6683301B2 (en) | 2001-01-29 | 2004-01-27 | Analytica Of Branford, Inc. | Charged particle trapping in near-surface potential wells |
US6707037B2 (en) | 2001-05-25 | 2004-03-16 | Analytica Of Branford, Inc. | Atmospheric and vacuum pressure MALDI ion source |
US6707036B2 (en) | 2002-03-21 | 2004-03-16 | Thermo Finnigan Llc | Ionization apparatus and method for mass spectrometer system |
US6707039B1 (en) | 2002-09-19 | 2004-03-16 | Agilent Technologies, Inc. | AP-MALDI target illumination device and method for using an AP-MALDI target illumination device |
-
2004
- 2004-06-07 CA CA2527886A patent/CA2527886C/fr not_active Expired - Lifetime
- 2004-06-07 EP EP04754443.2A patent/EP1639622B1/fr not_active Expired - Lifetime
- 2004-06-07 WO PCT/US2004/017832 patent/WO2004112074A2/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
EP1639622A4 (fr) | 2007-11-21 |
EP1639622B1 (fr) | 2016-11-16 |
WO2004112074A3 (fr) | 2005-10-06 |
WO2004112074A2 (fr) | 2004-12-23 |
CA2527886A1 (fr) | 2004-12-23 |
EP1639622A2 (fr) | 2006-03-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US7087898B2 (en) | Laser desorption ion source | |
US7375319B1 (en) | Laser desorption ion source | |
CA2527886C (fr) | Source d'ions de desorption laser | |
US6204500B1 (en) | Mass spectrometry from surfaces | |
US10593533B2 (en) | Imaging mass spectrometer | |
US6683301B2 (en) | Charged particle trapping in near-surface potential wells | |
US6952012B2 (en) | Ambient pressure matrix-assisted laser desorption ionization (MALDI) apparatus and method of analysis | |
US7109480B2 (en) | Ion source and methods for MALDI mass spectrometry | |
CA2448335C (fr) | Source d'ions maldi atmospherique et sous depression | |
US20110139977A1 (en) | Matrix-assisted laser desorption with high ionization yield | |
WO2002083275A9 (fr) | Procede et systeme de spectroscopie de masse | |
US6515280B1 (en) | Method and device for matrix assisted laser desorption ionization of substances | |
US20040056192A1 (en) | Ap-maldi target illumination device and method for using an ap-maldi target illumination device | |
CA2487136C (fr) | Spectrometrie de masse a multiples guides ioniques rf segmentes en plusieurs zones de pression |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |