EP1633192B1 - Antimicrobial composition comprising a polymeric biguanide and a copolymer and use thereof - Google Patents

Antimicrobial composition comprising a polymeric biguanide and a copolymer and use thereof Download PDF

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Publication number
EP1633192B1
EP1633192B1 EP04732131A EP04732131A EP1633192B1 EP 1633192 B1 EP1633192 B1 EP 1633192B1 EP 04732131 A EP04732131 A EP 04732131A EP 04732131 A EP04732131 A EP 04732131A EP 1633192 B1 EP1633192 B1 EP 1633192B1
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Prior art keywords
polymer
ionic
formula
composition according
derivatives
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German (de)
English (en)
French (fr)
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EP1633192A1 (en
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David John Hodge
David Alan Pears
John Jeffrey Gerrard
Paula Louise Mcgeechan
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Arch UK Biocides Ltd
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Arch UK Biocides Ltd
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Priority claimed from GB0325239A external-priority patent/GB0325239D0/en
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
    • A61L2/186—Peroxide solutions
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
    • A01N25/10—Macromolecular compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides
    • A01N47/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having a double or triple bond to nitrogen, e.g. cyanates, cyanamides containing —N=CX2 groups, e.g. isothiourea
    • A01N47/44—Guanidine; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L33/00—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical, or of salts, anhydrides, esters, amides, imides or nitriles thereof; Compositions of derivatives of such polymers
    • C08L33/04—Homopolymers or copolymers of esters
    • C08L33/06—Homopolymers or copolymers of esters of esters containing only carbon, hydrogen and oxygen, which oxygen atoms are present only as part of the carboxyl radical

Definitions

  • the present invention relates to a method for inhibiting the growth of micro-organisms on surfaces by means of a composition comprising a non-ionic vinyl comb type co-polymer and an antimicrobial agent.
  • the antimicrobial agent is controllably released from the vinyl co-polymer over time thereby providing effective anti-microbial control.
  • Micro-organisms can be found on many inanimate and animate surfaces. The presence of such micro-organisms can result in unhygienic conditions in hospitals and medical environments, kitchens, bathrooms, toilets and in the preparation and packaging of foodstuffs leading to health risks and contamination.
  • antimicrobial agents exist which are effective against many of the virulent forms of micro-organisms found in the food and health-care environments.
  • the activity of such agents is insufficient in terms of providing a sustained surface hygienic effect. This may be due to the high water solubility and/or lack of substantivity of the antimicrobial agent on a surface which means that the anti-microbial agents is readily displaced.
  • the literature describes various cases where micro-organisms and in particular bacterial fouling may cause damage or lead to contamination of surfaces including for example swimming pools, industrial pipes, architectural structures, ships hulls, hospital theatres, teeth and kitchen surfaces. Indeed, there have been many attempts and approaches to overcome the micro-biological problems associated especially with bacterial growth on inanimate and animate surfaces.
  • European Patent 0182523 describes how certain polymeric compositions are effective at preventing oral bacteria from colonisation on the surface of teeth.
  • an anti-staining composition comprising polymers with anti-bacterial agents were shown to be effective against bacteria found in an oral environment.
  • WO 00/02449 describes a process for the biocidal treatment of surfaces comprising high molecular weight grafted co-polymers.
  • sustained used hereinafter refers to an anti-microbial agent which is still active even after the surface to which the agent has been applied has been cleansed for example by wiping, rinsing or washing the surface.
  • non-ionic co-polymers provide effective and sustained antimicrobial activity when used to inhibit the growth of micro-organisms on surfaces.
  • the present invention therefore provides compositions for the treatment of surfaces based on non-ionic co-polymers with varying functionality in both the backbone and the side chain in combination with an anti-microbial agent, especially a biocide.
  • composition comprising:
  • a preferred anti-microbial agent for use in the composition according to the first aspect of the present invention is an anti-bacterial agent, more preferably a polymeric biguanide.
  • the polymeric biguanide comprises at least two biguanide units of Formula (2): linked by a bridging group which contains at least one methylene group.
  • the bridging group preferably includes a polymethylene chain, optionally incorporating or substituted by one or more hetero atoms such as oxygen, sulphur or nitrogen.
  • the bridging group may include one or more cyclic moieties which may be saturated or unsaturated.
  • the bridging group is such that there are at least three, and especially at least four, carbon atoms directly interposed between two adjacent biguanide units of Formula (2).
  • the polymeric biguanide may be terminated by any suitable group, such as a hydrocarbyl, substituted hydrocarbyl or an amine group or a cyanoguanidine group of the Formula (3):
  • the terminating group is hydrocarbyl, it is preferably alkyl, cycloalkyl, aryl or aralkyl.
  • the hydrocarbyl group is alkyl it may be linear or branched but is preferably linear.
  • Preferred alkyl groups include C 1-8 -alkyl.
  • Examples of preferred alkyl groups include for example methyl, ethyl, n-propyl, isopropyl, n-pentyl, n-butyl, isobutyl, tert -butyl and n-octyl.
  • hydrocarbyl group When the hydrocarbyl group is cycloalkyl, it is preferably cyclopropyl, cyclopentyl or cyclohexyl.
  • hydrocarbyl group When the hydrocarbyl group is aralkyl, it preferably contains from 1 to 6, more preferably 1 or 2 carbon atoms in the alkylene group attaching the aryl group to the biguanide.
  • Preferred aralkyl groups include benzyl and 2-phenylethyl groups.
  • Preferred aryl groups include phenyl groups.
  • the substituent may be any substituent that does not exhibit undesirable adverse effects on the microbiological properties of the polymeric biguanide. Examples of such substituents are aryloxy, alkoxy, acyl, acyloxy, halogen and nitrile.
  • the biguanide is a bisbiguanide.
  • the two biguanide groups are preferably linked through a polymethylene group, especially a hexamethylene group.
  • the polymeric biguanide preferably contains more than two biguanide units of Formula (1) and is preferably a linear polymeric biguanide which has a recurring polymeric chain represented by Formula (4) or a salt thereof: wherein d and e represent bridging groups which may be the same or different and in which together the total of the number of carbon atoms directly interposed between the pairs of nitrogen atoms linked by d plus the number of carbon atoms directly interposed between the pairs of nitrogen atoms linked by e is more than 9 and less than 17.
  • the bridging groups d and e preferably consist of polymethylene chains, optionally interrupted by hetero atoms, for example, oxygen, sulphur or nitrogen.
  • d and e may also incorporate moieties which may be saturated or unsaturated, in which case the number of carbon atoms directly interposed between the pairs of nitrogen atoms linked by d and e is taken as including that segment of the cyclic group, or groups, which is the shortest.
  • the number of carbon atoms directly interposed between the nitrogen atoms in the group is 4 and not 8.
  • the linear polymeric biguanides having a recurring polymer unit of Formula (4) are typically obtained as mixtures of polymers in which the polymer chains are of different lengths.
  • the number of individual biguanide units of Formulae (5a) and (5b): is, together, from 3 to about 80.
  • the preferred linear polymeric biguanide is a mixture of polymer chains in which d and e are identical and the individual polymer chains, excluding the terminating groups, are of the Formula (6) or a salt thereof: wherein n 1 is from 4 to 20 and especially from 4 to 18. It is especially preferred that the average value of n 1 is about 16.
  • the average molecular weight of the polymer in the free base form is from 1100 to 4000.
  • the linear polymeric biguanides may be prepared by the reaction of a bisdicyandiamide having the Formula (7): with a diamine H 2 N-e-NH 2 , wherein d and e have the meanings defined above, or, by the reaction between a diamine salt of dicyanamide having the Formula (8): with a diamine H 2 N-e-NH 2 wherein d and e have the meanings defined above.
  • polymer chains of the linear polymeric biguanides may be terminated either by an amino group or by a cyanoguanidine group of Formula (9):
  • This cyanoguanidine group can hydrolyse during preparation of the linear polymeric biguanide yielding a guanidine end group.
  • the terminating groups may be the same or different on each polymer chain.
  • a small proportion of a primary amine R-NH 2 may be included with the diamine H 2 N-e-NH 2 in the preparation of polymeric biguanides as described above.
  • the primary amine acts as a chain-terminating agent and consequently one or both ends of the polymeric biguanide polymer chains may be terminated by an -NHR group.
  • These -NHR chain-terminated polymeric biguanides may also be used.
  • the polymeric biguanides readily form salts with both inorganic and organic acids.
  • Preferred salts of the polymeric biguanide are water-soluble.
  • the polymeric biguanide used in accordance with the present invention is a mixture of linear polymers, the individual polymer chains of which, excluding the terminating groups, are represented by Formula (6) in the hydrochloride salt form.
  • This poly(hexamethylenebiguanide) compound is commercially available from Avecia Limited under the trademarks Vantocil TM , Cosmocil TM and Reputex TM .
  • Non-Ionic Vinyl Comb Type Co-polymers (Non-ionic co-polymers) .
  • non-ionic co-polymers of the present invention are as illustrated in the following Empirical Structural Formula.
  • non-ionic co-polymer referred to herein is used to describe a co-polymer which can be derived from an addition polymerisation reaction (that is, a free radical initiated process which can be carried out in either an aqueous or non aqueous medium) of two or more olefinically unsaturated monomers. Therefore, the term vinyl monomer used throughout refers to an olefinically unsaturated monomer.
  • Vinyl monomers with additional functionality for subsequent crosslinking of the films such as diacetone acrylamide, aceto acetoxy ethyl methacrylate, glycidyl (meth)acrylate, alkoxy 2-(trimethylsiloxy)ethyl methacrylate, 2-hydroxy ethyl (meth)acrylate, 4-hydroxy butyl (meth)acrylate, 3-hydroxy propyl (meth)acrylate, hydroxy stearyl (meth)acrylate, and 2-hydroxyethyl(meth)acrylate, can also be used.
  • diacetone acrylamide aceto acetoxy ethyl methacrylate
  • glycidyl (meth)acrylate alkoxy 2-(trimethylsiloxy)ethyl methacrylate
  • 2-hydroxy ethyl (meth)acrylate 4-hydroxy butyl (meth)acrylate
  • 3-hydroxy propyl (meth)acrylate hydroxy stearyl (meth)acrylate
  • a particularly preferred non-ionic co-polymer of the present invention is an acrylic co-polymer, derived from esters of acrylic or methacrylic acid.
  • non-ionic co-polymers of the present invention comprise at least one polymer which comprises one or more repeating units of Formula (1). wherein :
  • [B] provides the non-ionic functionality polyethylene oxide component of the non-ionic co-polymer and [A] is derived from any olefinically unsaturated polymerisable monomer which does not contain an ionisable or ionised functional group.
  • the molar ratio of [A] to [B] (m:n) is in the range 1:10 to 10:1, more preferably 1:1 to 10:1 and most preferably 2:1 to 4:1.
  • the molar ratios of monomers [A] to [B], (m:n) respectively are also chosen such that the cloud point of the non-ionic co-polymer is greater than 0°C more preferably greater than 15°C and most preferably greater than 25°C.
  • the cloud point value is related to the solubility of the co-polymer in water and refers to the boundary at which liquid-liquid phase separation takes place in a mixture of two or more components indicated by a cloudiness of the solution due to the formation of aggregates that scatter light.
  • the temperature at which a 1% by weight solution of a polymer in distilled water becomes cloudy is the cloud point temperature.
  • the polymer comprises 10 to 90% by weight polyethylene oxide provided by [B], more preferably 40 to 85% by weight and most preferably 40 to 75% by weight of [B].
  • the exact level of polyethylene oxide introduced by [B] required to achieve a cloud point in the preferred range depends on a number of factors for example:
  • the anti-microbial agent/non-ionic co-polymer compositions of the present invention form a clear solution, that is the cloud point of the non-ionic co-polymer in the presence of anti-microbial agent, for example poly(hexamethylene biguanide) (PHMB) is above 15°C and more preferably above 25°C.
  • anti-microbial agent for example poly(hexamethylene biguanide) (PHMB) is above 15°C and more preferably above 25°C.
  • the value of p is 3 to 50, preferably 3 to 40 and most preferably 3 to 25.
  • the value of q is 15 to 1000 most preferably 20 to 400.
  • R 1 , R 2 and R 3 are each independently H, optionally substituted C 1-20 -alkyl or C 3-20 -cycloalkyl.
  • R 1 , R 2 and R 3 are H, C 1-10 -alkyl or C 3-8 -cycloalkyl.
  • R 1 and R 2 are independently H or CH 3 .
  • R 3 is H or C 1-6 -alkyl and especially H or CH 3 .
  • R 4 and R 5 in repeating units of [X] may be the same or different, and are each independently H or C 1-4 -alkyl so long as at least one of R 4 and R 5 is H.
  • one of R 4 and R 5 is H when the other is -CH 3 or -C 2 H 5 with the result that [X] comprises oxyethylene units or a mixture of oxyethylene, oxypropylene and/or oxybutylene units.
  • R 4 and R 5 are both H, and [X] comprises oxyethylene units.
  • T is an optionally substituted substituent examples of which include CN, OH, F, Cl, Br, -OR 6 , -C(O)R 6 , -OC(O)R 6 , -C(O)OR 6 , -C(O)NR 7 R 8 and aryl optionally substituted by -OC(O)R 6 , F, Cl, Br, C 1-6 -alkyl, -CH 2 Cl or -C(O)OR 6 .
  • R 6 is C 1-10 -alkyl more preferably C 1-8 -alkyl for example methyl, ethyl, propyl, butyl, isopropyl, isobutyl or tert-butyl optionally substituted by a ketone, ether, epoxide, silane or ketoester group.
  • R 7 and R 8 are each independently H, C 1-8 -alkyl or C 3-8 -cycloalkyl optionally substituted by -OH, ketone or alkyl ether groups, most preferably R 7 and R 8 are H, -CH 3 or C 2 H 5 .
  • T is of the formula C(O)OR 6 , -C(O)NR 7 R 8 or -OC(O)R 6 and most preferably T is C(O)OR 6 , wherein R 6 , R 7 and R 8 are as previously described.
  • Each L is an optionally substituted linking group which joins [X] to the hydrocarbyl polymer backbone.
  • L can be a variety of linking groups and may be the same or different. Examples of L preferably comprise one or more carbon and/or hetero atoms, for example nitrogen or oxygen. Examples of preferred linking groups represented by L include: wherein the right hand side of the linking group is attached to [X] and the left hand side of the linking group is attached to the hydrocarbyl backbone.
  • each L is of formula
  • Examples of olefinically unsaturated monomers which may be used for [A] in Formula (1) include: styrene, ⁇ -methyl styrene, acrylonitrile, methacrylonitrile, vinyl halides such as vinyl chloride, vinyl esters such as vinyl acetate, vinyl propionate, vinyl laurate, and vinyl esters of versatic acid such as VeoVaTM 9 and VeoVaTM 10 (available from Resolution Performance Products), vinyl ethers of heterocyclic vinyl compounds, in particular, esters of acrylic acid and methacrylic acid.
  • Olefinically unsaturated monomers with additional functionality for subsequent crosslinking and/or adhesion promotion for use in the present invention may also be used, examples of which include diacetone acrylamide, acetoacetoxyethyl-methacrylate and glycidyl methacrylate.
  • Examples of olefinically unsaturated monomers which may be used for [B] in Formula (1) include: vinyl polyethers of ethylene or propylene oxide, for example hydroxypolyethoxy(5), polypropoxy(5), monoallyl ether (BX-AA-E5P5 available from Bimax Chemicals Ltd), methoxypolyethyleneglycol 350 methacrylate (available from Laporte under the trade name Bisomer MPEG350MA), methoxypolyethyleneglycol 550 methacrylate (available from Laporte under the trade name Bisomer MPEG550 MA), methoxypolyethyleneglycol 350 acrylate, polyethyleneglycol (6) methacrylate PEM6, polyethyleneglycol (6) acrylate PEA6.
  • vinyl polyethers of ethylene or propylene oxide for example hydroxypolyethoxy(5), polypropoxy(5), monoallyl ether (BX-AA-E5P5 available from Bimax Chemicals Ltd), methoxypolyethyleneglycol 350 methacrylate (available
  • Preferred non-ionic co-polymers for use in the present invention are based on acrylic co-polymers, that is co-polymers based on esters of acrylic or methacrylic acid.
  • [A] and [B] as defined in Formula (1) for use in the present invention have the Formulae (12) and (13) respectively wherein:
  • the molar ratios of [A] to [B], (m:n) respectively, are preferably chosen such that the cloud point of the non-ionic co-polymer is greater than 25°C.
  • Preferred olefinically unsaturated monomers which may be used for [A] of Formula (12) are therefore methyl acrylate, methyl methacrylate, ethyl acrylate, ethyl methacrylate, n-butyl acrylate, n-butyl methacrylate, 2-ethylhexyl acrylate, 2-ethylhexyl methacrylate, isopropyl acrylate, isopropyl methacrylate, n-propyl acrylate, n-propyl methacrylate, t-butyl methacrylate, 2-ethylhexyl methacrylate, isobornyl methacrylate, and cyclohexyl methacrylate and the corresponding acrylates.
  • Methacrylates or acrylates having optional substitution at R 6 such as for example epoxide, alkyl ether and aryl ether groups, hydroxyalkyl groups for example, hydroxyethyl, hydroxy propyl or hydroxy butyl and modified analogues, may also be employed as part of [A] of Formula (12).
  • Ketofunctional monomers such as the acetoacetoxy esters of hydroxyalkyl acrylates and methacrylates such as acetoacetoxyethyl methacrylate, as well as silane functional monomers such as alkoxysilane alkyl methacrylates may also be used.
  • the advantage of using a functionalised monomer for [A] is that it provides subsequent crosslinkability or adhesion promotion in the resulting polymer.
  • Examples of the preferred olefinically unsaturated monomers which may be used for [B] in Formula (13) are methoxypolyethyleneglycol 350 methacrylate (available from Laporte under the trade name Bisomer MPEG350 MA), hydroxy polyethyleneglycol 350 methacrylate, methoxypolyethyleneglycol 350 methacrylate (available from Laporte under the trade name Bisomer MPEG550 MA), methoxypolyethyleneglycol 550 acrylate, polyethyleneglycol (6) mono methacrylate PEM6, polyethyleneglycol (6) mono acrylate PEA6.
  • methoxypolyethyleneglycol 350 methacrylate available from Laporte under the trade name Bisomer MPEG350 MA
  • hydroxy polyethyleneglycol 350 methacrylate methoxypolyethyleneglycol 350 methacrylate
  • methoxypolyethyleneglycol 350 methacrylate available from Laporte under the trade name Bisomer MPEG550 MA
  • the non-ionic co-polymers of the present invention comprise a vinyl backbone with pendant side-chains.
  • the non-ionic co-polymers preferably comprises from 10% to 90% by weight of side chain monomer [B] and from 10% to 90% backbone monomer [A].
  • the non-ionic co-polymers used in the present invention may be prepared by any co-polymerisation method known in the art.
  • the co-polymerisation reaction is carried out in water, an organic solvent or a mixture of water and organic solvent using a free radical initiator.
  • Suitable free radical yielding initiators include inorganic peroxides for example potassium, sodium or ammonium persulphate, hydrogen peroxide, or percarbonates; organic peroxides, such as acyl peroxides including for example benzoyl peroxide, alkyl hydroperoxides such as t-butyl hydroperoxide and cumene hydroperoxide; dialkyl peroxides such as di-t-butyl peroxide; peroxy esters such as t-butyl perbenzoate and mixtures thereof may also be used.
  • the peroxy compounds are in some cases advantageously used in combination with suitable reducing agents (redox systems) such as sodium or potassium pyrosulphite or bisulphite, and iso-ascorbic acid.
  • Azo compounds such as azoisobutyronitrile or dimethyl 2,2'-azo bis-isobutylate may also be used.
  • Metal compounds such as iron-ethylenediamine tetracetic acid (EDTA) may also be usefully employed as part of the redox initiator system.
  • Other free radical initiators include: cobalt chelate complexes and particularly Co(II) and Co(III) complexes of porphyrins, dioximes and benzildioxime diboron compounds. It is also possible to use an initiator system partitioning between the aqueous and organic phases, for example a combination of t-butyl hydroperoxide, iso-ascorbic acid and iron-ethylenediamine tetracetic acid.
  • Preferred initiators comprise azo compounds such as azo-iso-butyronitrile and dimethyl 2,2'-azo bis-isobutylate and peroxides such as hydrogen peroxide or benzoyl peroxide.
  • the amount of initiator or initiator system conventionally used is for example within the range of from 0.05 to 6 weight % based on the total amount of vinyl monomers used, more preferably from 0.1 to 3% and most preferably from 0.5 to 2% by weight based on the total amount of vinyl monomers used.
  • the organic solvent is preferably a polar organic solvent for example a ketone, alcohol or an ether.
  • suitable polar solvents are methyl ethyl ketone, acetone, methyl isobutylketone, butyl acetate, ethoxyethylacetate, methanol, ethanol, n-propanol, iso-propanol, n-butanol, amyl alcohol, diethylglycol mono-n-butyl ether and butoxyethanol.
  • the polar organic solvent may be used with a non-polar organic liquid.
  • suitable non-polar organic solvents include: toluene-xylene mixtures and methylenechloride-dimethylformamide mixtures.
  • the co-polymerisation reaction is carried out in aqueous alcoholic solvents for example, methanol, ethanol, n-propanol, iso-propanol, n-butanol, amyl alcohol, diethylglycol or butoxyethanol, especially aqueous ethanol mixtures.
  • the number average molecular weight (Mn) of the non-ionic co-polymers is typically in the range 5,000 to 200,000, more preferably 10,000 to 100,000.
  • the non-ionic co-polymers can also be made by aqueous emulsion or suspension polymerisation (as described in Principles of Polymerisation, G. Odian, Wiley. Interscience 3rd Edition 1991 , incorporated herein by reference), in which case the Mn value may be higher and in the range 20,000 to 500,000.
  • a preferred anti-microbial agent for use in a composition with a non-ionic co-polymer as previously described comprises an antibacterial agent, more preferably a linear polymeric biguanide which is a mixture of polymer chains in which the individual polymer chains, excluding the terminating group are of Formula (6) or a salt thereof as hereinbefore described.
  • a preferred linear polymeric biguanide for use in the present invention is poly(hexamethylenebiguanide) hydrochloride (PHMB) available from Avecia Limited under the trade name Vantocil TM IB.
  • the amount of polymeric biguanide used in the composition of the present invention relative to the amount of non-ionic co-polymer is dependent upon the end use of the composition, the conditions under which it will be stored and the nature of the surface to which the composition is to be applied.
  • the weight ratio of the polymeric biguanide to non-ionic co-polymer in the composition may therefore vary over wide limits for example from 100:1 1 to 1:1000, more preferably from 20:1 to 1:500.
  • the ratio of the polymeric biguanide to non-ionic co-polymer in the antimicrobial composition is from 1:1 to 1:200.
  • the concentration of polymeric biguanide, for example poly(hexamethylene biguanide) (PHMB) used in the composition of the present invention is preferably in the range of from 0.001 % to 25%, more preferably 0.005% to 10%, and especially 0.01 % to 5%.
  • the pH of the composition is typically chosen so that it is most appropriate for a particular application and is preferably in the range of from 1 to 12, more preferably pH 3 to 9.
  • composition of the present invention may also contain other additives depending upon the particular use intended for the composition.
  • Additional components optionally included in the composition may be for example, additional polymeric materials, detergents, botanical extracts, perfumes, fragrances, thickeners, humectants, anti-corrosion agents, surfactants, colourants, chelating agents, buffers, acidity and alkalinity regulators, wetting agents, sequestering agents, hydrotropes, adjuvants, anti-soil agents and enzymes.
  • the carrier may be a solid but is preferably a liquid and the formulation is preferably a solution, suspension or emulsion of the antimicrobial composition in the liquid.
  • water is the preferred carrier for the composition
  • other solvents such as water miscible organic solvents may also be present in the composition.
  • suitable water-miscible organic solvents include: glycols such as ethylene glycol, propylene glycol, dipropylene glycol, methanol, ethanol, propan-1-ol, propan-2-ol, N-methyl-2-pyrrolidone and lower C 1-4 -alkyl carbitols such as methyl carbitol.
  • Preferred water-miscible organic solvents are glycols with 2 to 6 carbon atoms, poly-alkylene glycols with 4 to 9 carbon atoms or mono C 1-4 -alkyl ethers of glycols with 3 to 13 carbon atoms.
  • the most preferred water-miscible organic solvents are propylene glycol, ethyl hexyl glycol or ethanol or C 1-6 -alkyl esters for example butyl ethyl acetate or pentylacetate.
  • a preferred formulation of the final diluted application liquor according to a second aspect of the invention comprises from 0.01 to 5% by weight polymeric biguanide, more preferably from 0.1 to 1% by weight polymeric biguanide in the form of poly(hexamethylene biguanide) hydrochloride (PHMB).
  • the amount of non-ionic co-polymer in the formulation is preferably from 0.01 to 50% by weight, especially from 0.1 to 25% by weight.
  • the preferred carriers are water or water/alcohol mixtures.
  • the pH of the formulation is typically in the range of from 1 to 12, the pH chosen being that appropriate for the application. Most preferably the pH of the formulation is in the range of from 3 to 9.
  • An especially preferred formulation according to the second aspect of the present invention comprises a diluted application solution containing 0.5% by weight poly(hexamethylene biguanide) hydrochloride (PHMB) and from 2 to 15% by weight non-ionic co-polymer in the form of an aqueous solution.
  • PHMB poly(hexamethylene biguanide) hydrochloride
  • the formulation may also contain other additives depending upon the particular use intended for the composition. Additional additives optionally included in the formulation are for example those disclosed for use in compositions according to the first aspect of the invention.
  • a composition comprising a polymeric biguanide and a non-ionic co-polymer is applied to a surface a sustained anti-microbial effect against a broad range of micro-organisms including gram positive bacteria, gram negative bacteria, pathogenic bacteria, yeasts, fungi and algae is achieved. Therefore according to a further aspect of the present invention there is provided a method of treating a surface which comprises treating the surface with a composition or a formulation as hereinbefore described with reference to the first and second aspects of the present invention.
  • the preferred biguanide poly(hexamethylene biguanide) hydrochloride may be the only microbiologically active compound present in the composition or formulation. Alternatively, other microbiologically active compounds may also be present in combination with the polymeric biguanides.
  • microbiologically active compounds include for example: quaternary ammonium compounds for example, N,N-diethyl-N-dodecyl-N-benzylammonium chloride, N,N-dimethyl-N-octadecyl-N-(dimethyl benzyl) ammonium chloride, N,N-dimethyl-N,N-didecylammonium chloride, N,N-dimethyl-N,N-didodecylammonium chloride; N,N,N-trimethyl-N-tetradecylammonium chloride, N-benzyl-N,N-dimethyl-N-(C 12 -C 18 alkyl)ammonium chloride, N-(dichlorobenzyl)-N,-N-dimethyl-N-dodecylammonium chloride, N-hexadecylpyridinium chloride, N-hexadecyl pyridinium
  • the amount of further antimicrobial compound(s) in the composition will depend upon the further antimicrobial compound and the surface to be protected.
  • compositions or formulations of the present invention for disinfecting surfaces found in for example household, industrial or institutional areas.
  • the treatment can be applied to a wide variety of surfaces as exemplified as follows but not limited thereto.
  • Surface applications include for example, walls, floors, work surfaces, equipment found in domestic, industrial, food processing, sanitary, health and medical environments, skin, synthetic and natural textiles and fibres, stainless steel, polymer and polymeric coatings such as vinyl, polyvinyl chloride, polypropylene and polyethylene, wood, glass, rubber, paint surfaces, stone, marble, grouts, packaging and films.
  • the anti-microbial compositions according to the first and second aspects of the invention significantly reduce the levels of micro-organisms on surfaces treated with the anti-microbial compositions, which activity is sustained over a period of time.
  • a fourth aspect of the present invention there is therefore provided the use of a composition according to the first aspect of the present invention or the use of a formulation according to a second aspect of the present invention for the treatment of surfaces.
  • non-ionic co-polymers described above in relation to the present invention may also be used in combination with anti-fungal compounds. It has surprisingly been found that fungicidal compounds are also controllably released from the non-ionic co-polymers over time thereby providing sustained and effective anti-fungal control.
  • fungicides can be used in combination with the non-ionic co-polymers described above.
  • examples of such fungicides include but are not limited to:
  • Preferred antifungal compounds include for example quaternary ammonium compounds, isothiazolinone and benzisothiazolinone compounds, carbamates and pyridine compounds.
  • composition comprising:
  • Example 1 Preparation of Polymer 1 - A Non-Ionic Vinyl Copolymer.
  • a clean and dry two litre glass reactor was fitted with an overhead stirrer, nitrogen bleed, thermocouple and condenser.
  • An initiator solution [1] was prepared by dissolving dimethyl 2,2' azobis isobutyrate (2.3g) (0.01 moles) in solvent (97.3g of a 50/50 w/w mixture of ethanol /distilled water).
  • a monomer solution [2] was prepared containing solvent (490g of a 50/50 w/w mixture of ethanol/distilled water), methyl methacrylate (80g, 0.8 moles) and methoxy (polyethylene glycol 550) monomethacrylate (127g, 0.2 moles).
  • Monomer solution [2] was added to the reactor along with additional solvent (270g of a 50/50 w/w mixture of ethanol/distilled water) to help wash the monomers into the reactor.
  • the monomer solution [2] was washed into the reactor with additional solvent (100g of a 50/50 w/w mixture of ethanol/distilled water).
  • the reactor was heated to 75°C using a Haake circulating water bath and then stirred at 180 rpm under a nitrogen blanket.
  • At time zero initiator solution [1] (25g) was added to the reactor followed 30 minutes later by more initiator solution [1] (50g).
  • the reactor contents were left for 3 hours 30 minutes before increasing the reactor temperature to 80°C.
  • the total time of polymerisation was eight hours.
  • the final solution was water white and free of particulate matter.
  • the non-ionic co-polymer was formed in greater than 99% yield as determined by weight difference following exhaustive evaporation from a sample of the co-polymer solution.
  • the molecular weight of the co-polymer was determined using gel permeation chromatography (GPC) using polyethylene oxide as molecular weight standards. NMR Analysis was used to confirm the ratio of the repeat units [A], [B] and Dynamic Mechanical Thermal Analysis (DMTA) was used to determine the Tg of the co-polymer.
  • GPC gel permeation chromatography
  • DMTA Dynamic Mechanical Thermal Analysis
  • the value 1 corresponds to p in Formulae (11) and (13)
  • compositions 1 to 20 were prepared by mixing a 20% aqueous solution of poly (hexamethylenebiguanide) hydrochloride (PHMB) (5g), (available from Avecia Limited as Vantocil TM IB) to each of the polymers 1 to 8 from Table 1 as 20% solutions (water/ethanol 1/1) in varying quantities as set out in Table 2. The compositions were allowed to stand for 24 hours before being applied to substrates such as glass or ceramic tiles.
  • PHMB poly (hexamethylenebiguanide) hydrochloride
  • compositions were low viscosity colourless transparent solutions, free from sediment and with excellent storage stability. Storage stability was tested by storing the compositions for 2 months at 52°C and was considered excellent if the viscosity of the composition remained unchanged and there was no formation of precipitate or gel particles.
  • compositions 21-28 were prepared by mixing with various biocides. To a sample of the polymer solution the biocide was added at concentrations ranging from 0.1 % - 0.5% wt/wt on total weight of the solution. The compositions were placed on a rotating mixer for 24 hours to form a homogenous composition and then applied to substrates such as glass or ceramic tile. The compositions were of low viscosity and free from sediment. Table 2. Compositions of the Non-ionic Co-polymer /PHMB Formulations.
  • Non-Ionic Co-polymer (Table 1) Non-Ionic Co-polymer (wt%) Polymeric biguanide (PHMB) other Biocide (wt%) 1 1 37 63 2 1 50 50 3 1 55 45 4 1 71 29 5 1 74 26 6 1 80 20 7 1 83.3 16.7 8 1 85 15 9 1 95 5 10 2 83.3 16.7 11 2 95 5 12 3 83.3 16.7 13 3 95 5 14 4 83.3 16.7 15 4 95 5 16 5 95 5 17 6 95 5 18 7 95 5 19 8 95 5 20 9 90 10 21 4 99.9 0.1 of Biocide A 22 4 99.8 0.2 of Biocide a 23 4 99.8 0.2 of Biocide B 24 4 99.5 0.5 of biocide B 25 4 99.9 0.1 of Biocide C 26 4 99.8 0.2 of Biocide C 27 4 99.9 0.1 of biocide D 28 4 99.8 0.2 of Biocide D Biocide A n Butyl 1,2, benzisothiazolin Biocide
  • UV absorbance at 236nm of a known concentration of poly(hexamethylene biguanide) (PHMB) dissolved in water was measured (Perkin Elmer Lambda 900 UVNis/NIR Spectrophotometer).
  • UV absorbance at 236nm was measured for a series of samples prepared from known dilutions of the original PHMB aqueous solution.
  • a calibration curve for PHMB concentration in aqueous solution was produced (Graph 1) by plotting UV absorbance against PHMB concentration.
  • compositions 1 to 20 were separately applied to clean glass panels (150mm x 100mm) and films were drawn down using a Sheen 250 ⁇ down bar. The films were allowed to dry and the coating weight noted.
  • Each coated glass panel was immersed separately in distilled water (1L) in a 2L beaker and stirred at a constant speed using a magnetic stirrer.
  • Samples (approximately 5cm 3 ) were taken from the beaker in duplicate at regular intervals over the period of one hour. The samples were analysed using a UV spectrophotometer and the absorbance of each sample measured at a specific peak corresponding to the ⁇ max of the poly(hexamethylene biguanide) (PHMB). The measured absorbance was directly related to the concentration of the poly(hexamethylene biguanide) (PHMB) in the solution.
  • the rate of dissolution of poly(hexamethylene biguanide) from the non-ionic co-polymers could be controlled according to the co-polymer structure and by the ratio of non-ionic co-polymer to poly(hexamethylene biguanide).
  • the above illustrates that stable non-ionic co-polymer solutions in both water and water/ethanol mixture, can be prepared with polymeric biguanides such as poly(hexamethylene biguanide) hydrochloride.
  • compositions of Polymer [1] 1 with various levels of PHMB were evaluated by measuring Minimum Inhibitory Concentrations (MICs).
  • Non-ionic co-polymer/PHMB compositions were prepared as previously described (Table 2).
  • the residual antibacterial activity of the samples was evaluated by the following methodology.
  • the RABIT TM (Rapid Automated Bacterial Impedance Technique) measures the change in conductance of a bacterial suspension over time. Actively growing bacteria break down uncharged or weakly charged molecules in a defined media to give end products that are highly charged. The resultant increase in conductance can be directly related to bacterial concentration by the use of a calibration curve. (For further background on this technique see: 'Technical Reference Paper - RAB-03, Don Whitley Scientific, 14 Otley Road, Shipley, West Yorkshire, UK, BD17 7SE ).
  • Table 6 summarises the sustained bacterial activity of the non-ionic co-polymer/PHMB formulations obtained using the above techniques.
  • Table 6 Sustained Bactericidal Activity of Non-ionic Co-polymer (Example 1) with PHMB. Composition Number % By Weight PHMB on Polymer [1] 1 Log reduction versus Ps.aeruginosa after 5 minutes contact time No washes 1 wash 2 washes 100 PHMB (Control) 6.6 4.6 0.2 1 63 6.6 6.9 0.7 3 45 6.6 6.9 1 4 29 6.6 3.0 0.5 5 26 6.6 1.8 0.5 6 20 6.6 1.4 0.5 8 15 6.6 1.0 0.5
  • Table 6 demonstrates the sustained effect of poly(hexamethylene biguanide)(PHMB)/non-ionic co-polymer compositions.
  • the control sample (with PHMB alone) achieved a log 6.6 reduction in bacteria value initially, log 4.6 reduction after one wash and log 0.2 after 2 washes. Whilst this data demonstrates the effectiveness of PHMB in disinfection applications (equivalent to zero washes data), sustained effect behaviour is demonstrated by the increased log kill values after 1 and 2 washes.
  • composition 3 Co-polymer [1] achieved log 6.6, log 6.9 and log 1 reductions after 0, 1 and 2 washes respectively.
  • 45% PHMB is close to optimal in achieving a sustained antimicrobial effect.
  • Other PHMB/non-ionic co-polymer combinations have maximum sustained effect values at different ratios.
  • Table 7 Sustained Bactericidal Activity of Non-ionic Co-polymer (Example 3 & 9) with PHMB.
  • Table 7 illustrates that for a composition containing PHMB , sustained bactericidal activity is maximised in composition 13 for which log reductions of 6.9, 8.9 and 2.2 are achieved after 0, 1 and 2 washes respectively.
  • This formulation demonstrates improved sustained biocidal effect over both the control (PHMB alone which gives log reductions of 6.6, 4.6 and 0.2 after 0, 1 and 2 washes respectively) and the PHMB/Polymer [1] formulations (Table 6).
  • Non-ionic co-polymer/Biocide compositions were prepared as previously described (Table 2).
  • the residual antifungal activity of the samples was determined by the following methodology:
  • Table 8 summarises the sustained fungicidal activity of the Non-ionic co-polymer/Biocide formulations obtained using the above technique.
  • Table 8 Sustained Fungicidal Activity of Non-ionic Co-polymers (Example 4) with Various Biocides.
  • Composition Number Biocide Weight Ratio (w/w) Biocide polymer log reduction vs. A.niger @ 24h after no. of washes; 0 10 22 Biocide A 499:1 1.7 0.2 24 Biocide B 199:1 3.4 3.1 26 Biocide C 499:1 3.4 3.4 28 Biocide D 499:1 3.4 3.4

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US10093811B2 (en) 2016-07-11 2018-10-09 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems
US10759949B2 (en) 2016-07-11 2020-09-01 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems

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CA2613005C (en) * 2005-06-27 2017-01-17 Smith & Nephew Plc Antimicrobial biguanide metal complexes
NZ600951A (en) * 2010-01-04 2014-12-24 Basf Se Preservative formulation for cellulose containing materials
JP5837346B2 (ja) * 2011-07-05 2015-12-24 攝津製油株式会社 ウイルス不活化組成物
WO2013121222A1 (en) * 2012-02-16 2013-08-22 Arcis Biotechnology Limited Coating compositions and methods
CA2952867C (en) 2013-06-18 2022-05-03 Chemgreen Innovation Inc. An antimicrobial polymer wherein an aromatic moiety is covalently incorporated into the polymer backbone through loss of aromaticity
NZ631278A (en) * 2013-06-26 2018-06-29 Dermcare Vet Pty Ltd Antimicrobial compositions and methods of use
JP2017206441A (ja) * 2014-09-22 2017-11-24 Jsr株式会社 殺菌性組成物

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Publication number Priority date Publication date Assignee Title
US10093811B2 (en) 2016-07-11 2018-10-09 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems
US10759948B2 (en) 2016-07-11 2020-09-01 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems
US10759949B2 (en) 2016-07-11 2020-09-01 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems
US11274215B2 (en) 2016-07-11 2022-03-15 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems
US11286393B2 (en) 2016-07-11 2022-03-29 Spartan Chemical Company, Inc. Antimicrobial sacrificial floor coating systems

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