Classifications

• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
  • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
  • B01L3/508—Rigid containers without fluid transport within
  • B01L3/5085—Rigid containers without fluid transport within for multiple samples, e.g. microtitration plates
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
  • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
  • B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
  • B29C45/14—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/12—Specific details about manufacturing devices
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
  • B01L2200/14—Process control and prevention of errors
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
  • B01L2300/00—Additional constructional details
  • B01L2300/08—Geometry, shape and general structure
  • B01L2300/0809—Geometry, shape and general structure rectangular shaped
  • B01L2300/0829—Multi-well plates; Microtitration plates
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
  • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
  • B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
  • B29C45/14—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor incorporating preformed parts or layers, e.g. injection moulding around inserts or for coating articles
  • B29C2045/1486—Details, accessories and auxiliary operations
  • B29C2045/14967—Injecting through an opening of the insert
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
  • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
  • B29C45/00—Injection moulding, i.e. forcing the required volume of moulding material through a nozzle into a closed mould; Apparatus therefor
  • B29C45/0025—Preventing defects on the moulded article, e.g. weld lines, shrinkage marks
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
  • B29K—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES B29B, B29C OR B29D, RELATING TO MOULDING MATERIALS OR TO MATERIALS FOR MOULDS, REINFORCEMENTS, FILLERS OR PREFORMED PARTS, e.g. INSERTS
  • B29K2705/00—Use of metals, their alloys or their compounds, for preformed parts, e.g. for inserts

Definitions

• the present invention relates to a structure of a container and a method of manufacturing the same and, more specifically, to a structure of a microplate having a plurality of wells for storing samples to be observed in the field of biology, medical science, chemistry and the like and a method of manufacturing the same.
• a microplate formed by adhering a plate having a light transmissible property on one of the surfaces of a plastic resin plate having a metal plate sandwiched therein.
• the metal plate since the metal plate has a good thermal conductivity, it is easy to heat or cool the samples in a state of being stored in the microplate or to provide the respective samples on the identical microplate temperature gradient, and is preferable in observation of samples based on a PCR (Polymerase Chain Reaction) method.
• Fig. 9 An example of the structure of such a microplate in the related art is shown in Fig. 9. In Fig.
• FIG. 9 (a) is a top view of such a microplate 1, (b) is a bottom view of the microplate 1, (c) is an enlarged top view of the microplate 1, and (d) is a cross-sectional view of the microplate 1 taken along an alternate long and short dash line shown as C-C in (c) .
• an outer frame which does not relate to the present invention is omitted.
• the microplate 1 includes cylindrical wells 2 arranged in rows and columns connected in a rectangular shape at regular intervals, as shown in Fig. 9(a) and (b) . Also, as shown in Fig.
• the microplate 1 is formed by adhering a cover glass 4 having light transmissibility with adhesive on one surface of a resin plate 3.
• the resin plate 3 is formed of resin 5, which is a plastic material.
• the resin plate 3 there is provided an aluminum plate 6, and the aluminum plate 6 covered by resin 5 constitutes the resin plate 3.
• the aluminum plate 6 is formed with round holes of larger diameter than the inner diameter of the well 2 at intervals corresponding to the arrangement of the wells 2 so as to prevent the aluminum plate 6 frombeing exposed inside the well 2 andcoming into direct contactwith the samples .
• the resin plate 3 is a plate shape member including the wells 2 connected with each other at the positions of the round holes provided on the aluminum plate 6.
• Fig. 10 an example of usage of the microplate 1 will be described.
• sample containing liquid 7 in which a sample is mixed is injected into the wells 2 on the microplate 1.
• an objective lens 8 is arranged below the cover glass 4, and observation of the sample is carried out through the cover glass 4.
• the microplate 1 having the aluminum plate 6 sandwiched therein may be fabricated, for example, by injecting and molding plastic resin in a forming die in which the aluminum plate 6 is set to form the resin plate 3, and then adhering the cover glass 4 on one side of the surfaces of the resin plate 3.
• a microplate formed with a metal plate sandwiched by plastic resin is disclosed, for example, in this document .
• Pamphlet of International PublicationNO.01/94018 A gate for injecting plastic resin into a cavity of a forming die for the above-described resin plate 3 is arranged, for example, in the vicinity of the center of the surface of the resin plate 3.
• deformation of the resinplate 3 due to uneven shrinkage upon hardening of plastic resin may occur on the front and back surfaces of the resin plate 3.
• the gate for injecting plastic resin since the gate for injecting plastic resin is disposed at the above-described position, the cooling speed on the surface of the resin plate 3 formed of plastic resin is different between the surface on the side provided with the gate and the surface on the opposite side. Therefore, warpingmay occur on the resi plate 3 because hardening of plastic resin does not proceed uniformly on both surfaces of the plate, and hence the flatness of the plate surface may be deteriorated. The warping may also occur when the density of plastic resin injected in the forming die is significantly different depending on the position of the forming die.
• the thickness of the cover glass 4 to be adhered to the resin plate 3 is generally very thin, and it may be broken when being deformed. Therefore, highly precise flatness is required for the adhesion surface of the light transmissible plate of the plastic resin plate.
• a problem to be solved by the present invention is to improve the flatness of the surface of the plastic resin plate, which includes a metal plate sandwiched therein and is formed into a microplate by adhering a light transmissible plate, to be adhered to the light transmissible plate. Disclosure of Invention A microplate according to the present invention has a plurality of wells for storing samples to be observed.
• a microplate according to a first embodiment of the present invention includes wells formed by covering openings of through-holes with a light transmissible plate having a light transmissible property on one side, the through-holes being formed on a resin plate formed by covering ametal plate withplastic resin and extending through the resin plate including the metal plate, and is characterized in that an additional through-hole is formed on the surface of the metal plate within the well-formed range but at the positions where the wells are not formed.
• Inthemicroplate accordingto the first embodiment of the present invention described above by employing an aluminum plate as the aforementioned metal plate, heating and cooling of the samples in the state of being stored in the microplate may be efficiently carried out owing to a good thermal conductivity of aluminum.
• Inthemicroplate according to the first embodiment of the present invention described above by employing duralumin alloy as the aforementioned metal plate, efficient heating and cooling of the samples is enabled, and in addition, a high rigidity may be provided to the microplate.
• the resinplate is formed by injecting plastic resin from one side of the metal plate, and the additional through-hole may be provided at the position nearest to the position of injection gate of plastic resin when the resin plate is formed.
• aplural number of the aforementioned additional through-holes may be provided.
• the resin plate is formed by injecting and molding plastic resin from one side of the metal plate, and the size of the through-hole located at the position closest to the position of injection gate of plastic resin when forming the resin plate is larger than the other additional through-holes.
• plastic resin is injected from one side of the meal plate disposed in the forming die, flowing of the resin into the opposite area of the plate is further facilitated.
• the resinplate is formed by injecting-molding plastic resin from one side of the metal plate, and the sizes of the additional through-holes other than that formed at the position nearest to the position of injection gate of plastic resin when forming the resin plate may be increased with increase in the distance from the position of injection gate.
• Flowability of resin is lowered with the distance from the position of injection gate of plastic resin since the temperature is lowered correspondingly. Therefore, by employing a structure in which the sizes of the "additional through-holes" on the metal plate is increased with increase in distance from the position of injection gate of plastic resin, sufficient flowing amount of plastic resin toward the opposite area may be secured even though flowability is lowered.
• the aforementioned structure may be such that the resin plate is formed by injecting and molding plastic resin from one side of the metal plate, the wells are arranged at regular intervals in the vertical and lateral directions into a rectangular shape, the additional through-holes provided at the positions other than the nearest position to the position of injection gate of plastic resin when forming the resinplate are displaced from the positions at equal distance from the four wells providedin thevicinityof the additional through-holes, and the amount of displacement of the positions is based on the distance from the position of injection gate. Since the temperature goes down easily in the peripheral portion of the forming die used for injection-molding of the resin plate, the temperature of plastic resin in the corresponding portion is lower than the temperature thereof at the center of the forming die where plastic resin is injected.
• the ends of the aforementioned additional through-holes may have a round shape.
• the round hole which can easily be formed by machining the metal plate may be used as the "additional through-holes".
• a microplate according to a second embodiment of the present invention includes wells formed by covering openings of the through-holes with a light transmissible plate having a light transmissible property on one side, the through-hole being formed on a resin plate formed by covering ametal plate withplastic resin and extending through the resin plate including the metal plate, and is characterized in that at least one of the through-holes provided on the metal plate passes through the plurality of wells.
• the through-hole provided on the surface of the metal plate describedabove, whichpasses through theplurality of wells may have a rectangular shape.
• the microplate according to the second embodiment of the present invention described above may be constructed such that the aforementioned plate is formed by injecting and molding plastic resin from one side of the metal plate, and the sizes of the through-holes, which are formed on the surface of the metal plate so as to pass through the plurality of wells may be increased with increase in distance from the position of in ection gate. Flowability of resin is lowered with the distance form the position of injection gate of plastic resin since the temperature thereof is lowered correspondingly. Therefore, by employing a structure in which the sizes of the "through-holes passing through the plurality of wells" on the metal plate increase with increase in distance from the position of injection gate of plastic resin, sufficient flowing amount of plastic resin into the opposite area of the metal plate is secured even when flowability is lowered.
• Amethodof formingamicroplate includingthe steps of disposing the metal plate formed with the through-holes at the positions where the wells are formed and at the positions different from the positions where the wells are formed in the forming die, injecting and molding plastic resin from the position closest to one of the through-holes which are located at a position different from the positions where the wells are formed on the metal plate disposed in the forming die to form the metal plate into a resin plate covered- with the aforementioned plastic resin and formed with the through-holes at the positions where the wells are to be formed, and adhering a light transmissible plate having a light transmissible property to one side of the resin plate to cover the through-holes formed on the resin plate on one side, thereby forming the wells, is also included in the present invention, and the microplate according to the first embodiment of the present invention described above may be manufactured by executing this manufacturing method.
• Amethodof forming amicroplate includingthe steps of disposing a metal plate formed with through-holes in a forming die, injecting and molding plastic resin in the forming die to form the metal plate into a resin plate covered with the aforementioned plastic resin and formed with the through-holes, the meal plate including at least one through-hole passing through a plural number of through-holes provided on the resin plate, adhering a light transmissible plate having a light transmissible property to one side of the resin plate to cover the through-holes formed on the resin plate on one side, thereby forming the wells, is also included in the present invention, and the microplate according to the second embodiment of the present invention described above is manufactured by carrying out this manufacturing method.
• Fig. 1 is a drawing showing a first example of a microplate according to the present invention.
• Fig. 2 is a drawing showing the structure of the aluminum plate used in the microplate shown in Fig. 1.
• Fig. 3 is a drawing showing a second example of the microplate according to the present invention.
• Fig.4 is a drawing showing a third example of the microplate according to the present invention.
• Fig. 5 is a drawing showing a fourth example of the microplate according to the present invention.
• Fig.6 is a drawing showing an example of the shape of a flow-through-hole in cross section.
• Fig.7 is a drawing showing a fifth example of the microplate according to the present invention.
• Fig. 1 is a drawing showing a first example of a microplate according to the present invention.
• Fig. 2 is a drawing showing the structure of the aluminum plate used in the microplate shown in Fig. 1.
• Fig. 3 is a drawing showing a second example of the microplate according
• FIG. 8 is a drawing showing the structure of an aluminum plate used in the microplate shown in Fig. 7.
• Fig. 9 is a drawing showing an example of the structure of the microplate in the related art.
• Fig. 10 is a drawing showing an example of usage of the microplate.
• FIG. 1 shows a first example of a microplate according to the present invention, in which (a) is a partially enlarged top view of a microplate 1 according to the present invention, and (b) is a cross-sectional view taken along an alternate long and short dash line shown as C-C in (a) , respectively.
• Themicroplate 1 according to thepresent invention includes cylindrical wells 2 arranged in rows and columns connected in a rectangular shape at regular intervals as in the related art shown in Fig. 9. As shown in Fig. 1 (b) , it is also the same as the related art, shown in Fig.
• a cover glass 4 which is a plate having a light transmissible property, is adhered to one side of a resin plate 3 with adhesive and hence the openings of the cylindrical through-holes provided on the resin plate 3 is covered with the cover glass 4 on one side, whereby the microplate 1 is formed.
• the resin plate 3 is formed of resin 5, which is plasticmaterial, and includes an aluminumplate 6, which is a metal plate having good thermal conductivity, disposed therein. In other words, the resin plate 3 is formed by covering the aluminum plate 6 with resin 5.
• the structure of the aluminum plate 6 used in the microplate 1 shown in Fig. 1 will be shown in Fig. 2.
• the aluminum plate 6 is formed with through-holes (well through-holes 11) having a diameter larger than the inner diameter of the well 2 at intervals corresponding to the arrangement of the cylinders so as to prevent the aluminum plate 6 from being exposed inside the well 2 and coming into direct contact with the samples, and the cylinders formed by covering the aluminum plate 6 with resin 5 corresponds to the side walls of the wells 2.
• the resin plate 3 having the aluminum plate 6 sandwiched therein as shown in Fig. 1 is formed by disposing the aluminum plate 6 in the forming die, and injecting andmolding resin 5 fromone side of the aluminum plate 6 in the forming die.
• the aluminum plate 6 is characterized in that the through-hole referred to as flow-through-hole 12 is provided in addition to the well through-holes 11.
• the effect of the flow-through-hole 12 is remarkable when being disposed at the position closer to the position of injection gate (the position of the gate in the forming die) when injecting resin
• duralumin alloy aluminum alloy containing copper, magnesium, manganese, etc.
• rigidity of the resin plate 3 as well as the microplate 1 may be improved.
• the flow-through-hole 12 provided on the aluminum plate 6 is shown in the state of being viewed through the resin 5 having a light transmissible property and the flow-through-hole 12 is not required to be formedthroughthe resinplate 3 itself.
• FIG. 3 shows a second example of the microplate 1 according to the present invention.
• the example shown in Fig. 3 is characterized in that when the microplate 1 is formed, the flow-through-hole 12 is positioned at the same distance from any of the four wells 2 formed into a square shape of two rows and two columns in the vicinity of the flow-through-hole 12, more specifically, is positioned at the center of gravity of the square having apexes at the centers of the four wells 2.
• the one disposedat thepositionnearest to the position of injection gate of resin 5 when the aluminum plate 6 is disposed at a predetermined position in the forming die for forming the resin plate 3 is preferably formed to have a diameter larger than those of other flow-through-holes 12.
• plastic resin when plastic resin is injected from one side of the aluminum plate 6 disposed in the forming die, flowing of resin into the opposite area thereof may further be improved.
• the hole diameter of the flow-through-holes other than that disposed to the positionnearest to the position of injection gate of resin 5 when the aluminum plate 6 is disposed at the predeterminedposition in the forming die for forming the resin plate 3 is preferably increased with increase in distance from the position of injection gate.
• flowability of resin 5 in the forming die is lowered with increase in distance from the position of injection gate of resin 5
• a sufficient amount of resin 5 flowing into the opposite area of the aluminum plate 6 is secured even though flowability of resin 5 is lowered by determining the hole diameter of the flow-through-holes 12 provided on the aluminum plate 6 so as to increase with increase in distance from the position of injection gate of resin 5.
• the flow-through-holes 12 other than that disposed at the position nearest to the position of injection gate of resin 5 when the aluminum plate 6 is disposed at the predetermined position in the forming die for forming the resinplate 3 is preferably arranged at the position displaced from the position at an equal distance from the four wells 2 provided in the vicinity of the flow-through-holes 12.
• the microplate 1 in this structure will be described based on Fig. 4.
• the flow-through-hole 12 is provided at the position displaced from the position of the center of gravity of a square having apexes at the centers of the four wells 2 on the aluminum plate 6. More specifically, with respect to the four wells 2, the flow-through-hole 12 is provided at the position closest to the upper right well 2 which is farthest from the gate position 21, second closest to the lower right well 2 which is second farthest from the gate position 21, third closest to the upper left well 2 which is third farthest from the gate position 21, and farthest to the lower left well 2 which is closest to the gate position 21.
• flowing balance of resin 5 for forming the four wells 2 is uniformized and, consequently, flatness of the resin plate 3 on the surface to be adhered to the cover glass 4 is improved.
• the amount of displacement of the position of the flow-through-hole 12 on the aluminum plate 6 is determined based on the distance L from the aforementioned gate position 21.
• the flow-through-holes 12 on the aluminum plate 6 described above in conjunction with Fig. 1, Fig. 3, or Fig.4 are all through-holes ofroundshape. Thereason why these flow-through-holes 12 are round in the aforementioned embodiments is because machining for providing the flow-through-hole 12 on the aluminumplate 6 is easy, and the shape of the flow-through-hole 12 is not limited.
• the shape of the flow-through-hole 12 may be formed into a shape shown in Fig.5, that is, in the case where the flow-through-hole 12 is arranged at the position at an equal distance from any of the four wells 2 disposed in the vicinity of the flow-through-hole 12 into a square of two rows and two columns, the shape of the portion which is not included in circular arcs of the same radius from the four respective wells 2 may be employed as a shape of the flow-through-hole 12.
• the distance from the respective portions of the wells 2 to the flow-through-hole 12 is equalized, and hence flatness of the surface of the resin plate 3 to be adhered to the cover glass 4 is further improved.
• the flow-through-hole 12 may be formed into a tapered shape which is decreased in diameter toward the direction of flow of resin 5 as shown in (a) in Fig.
• FIG. 6 which shows examples of the shape of the flow-through-hole 12 in cross section.
• flowability of resin 5 to the opposite area of the aluminum plate 6 may further be improved.
• the end portion of the flow-through-hole 12 may be chamfered, and whereby flowabilityof resin 5 to the opposite area of the aluminum plate 6 may further be improved.
• Fig. 6(b) shows a case in which the end portion of the flow-through-hole 12 is chamfered into a rounded shape, it may be chamfered into an angular shape.
• Fig. 7 will be described. Fig.
• the microplate 1 includes the cylindrical wells 2 arranged in rows and columns connected in a rectangular shape at regular intervals as in the related art shown in Fig. 9. It is the same in the structure as the related art shown in Fig. 9 in that the cover glass 4 is a plate having a light transmissible property is adhered to one side of the resin plate 3 with adhesive, and the openings of the cylindrical through-holes provided on the resin plate 3 are covered by the cover glass 4 on one side form the microplate 1.
• the resin plate 3 is formed of resin 5, which is plastic material, and includes the aluminum plate 6 is a metal plate having good thermal conductivity disposed therein.
• the resin plate 3 is formed by covering the aluminum plate 6 with resin 5.
• the structure of the aluminum plate 6 used in the microplate 1 shown in Fig. 7 will be shown in Fig. 8.
• the aluminum plate 6 is formed with through-holes (well through-holes 11) of larger diameter than the inner diameter of the well 2 at intervals according to the arrangement of the cylinders so as toprevent the aluminum plate 6 from being exposed inside the well 2 and coming into direct contact with the samples, and the cylinder formed by covering the aluminum plate 6 with resin 5 corresponds to the side walls of the wells 2.
• the aluminum plate 6 shown in Fig. 8 is provided with the flow-through-hole 12 in addition to the well through-holes 11.
• the flow-through-hole 12 on the aluminum plate 6 shown in Fig. 8 differs from that shown in Fig.2, and has a significant characteristic in that it has a diameter of the size that can pass through a plurality (two in the example in Fig. 7) of wells 2.
• the flow-through-hole 12 of such a size is provided on the aluminum plate 6, when resin 5 is injected from one side of the aluminum plate 6 disposed in the forming die when forming the resin plate 3, melted resin 5 passes through the flow-through-hole 12 and easily flown into the opposite area thereof. Therefore, flowing balance of resin 5 in the forming die at the time of injection-molding of the resin plate 3 is uniformized, and hence flatness of the surface of the resin plate 3 to be adhered to the cover glass 4 is improved.
• the flow-through-hole 12 provided on the aluminum plate 6 shown in Fig. 8 is a rectangular shape. It is because it does not affect the arrangement .of the wells
• the sizes of the flow-through-holes 12 preferably increase with increase in distance from the position of injection gate of resin 5 at the time when the aluminumplate 6 is disposed at the predetermined position in the forming die for forming the resin plate
• the sufficient amount of resin 5 flowing to the opposite area of the aluminum plate 6 may be secured by increasing the size of the flow-through-holes 12 to be provided on the aluminum plate 6 as described above with increase in distance from the position of inj ection gate evenwhen flowability of resin 5 is lowered.
• the present invention is not limited to the aforementioned embodiments, and may be improved or modified without departing the scope of the invention.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• Manufacturing & Machinery (AREA)
• Mechanical Engineering (AREA)
• Analytical Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Hematology (AREA)
• Clinical Laboratory Science (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Optical Measuring Cells (AREA)
• Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Applications Claiming Priority (2)

Publications (1)

Family

ID=34074689

Family Applications (1)

Country Status (3)

Families Citing this family (1)

Family Cites Families (3)

• 2003
  • 2003-07-23 JP JP2003278093A patent/JP2005043236A/ja not_active Withdrawn
• 2004
  • 2004-07-22 WO PCT/JP2004/010794 patent/WO2005007291A1/en not_active Ceased
  • 2004-07-22 EP EP04748058A patent/EP1631386A1/de not_active Withdrawn

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events