EP1629788A1 - Micro-pipette avec ouverture oblique - Google Patents
Micro-pipette avec ouverture oblique Download PDFInfo
- Publication number
- EP1629788A1 EP1629788A1 EP03732175A EP03732175A EP1629788A1 EP 1629788 A1 EP1629788 A1 EP 1629788A1 EP 03732175 A EP03732175 A EP 03732175A EP 03732175 A EP03732175 A EP 03732175A EP 1629788 A1 EP1629788 A1 EP 1629788A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- micro
- holding
- embryo
- holding pipette
- pipette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/42—Gynaecological or obstetrical instruments or methods
- A61B17/425—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation
- A61B17/435—Gynaecological or obstetrical instruments or methods for reproduction or fertilisation for embryo or ova transplantation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
- B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
- B01L3/022—Capillary pipettes, i.e. having very small bore
Definitions
- the present invention relates to a micro-holding pipette.
- a micro-holding pipette is a micropipette that is used to hold or fix an ovum or embryo of human or animals in microscope by negative pressure.
- all kinds of micro-holding pipette have blunt ends, i.e. their surfaces are at a blunt angle (90°) with the ordinate axis of the holding end (Fig.1 and Fig.2).
- Pipettes are widely used in assisted reproductive technology (ART), for example, intracytoplasmic sperm injection (ICSI), assisted hatching (AH) and preimplantation genetics diagnosis (PGD).
- ART assisted reproductive technology
- ICSI intracytoplasmic sperm injection
- AH assisted hatching
- PTD preimplantation genetics diagnosis
- the ovum or embryo is held at a 9 o'clock position by a micro-holding pipette and another micropipette, a ICSI needle or a hatching needle opposite to the micro-holding pipette, pierces the zona pellucid (ZP) of the ovum or embryo at a 3 o'clock position during micro-processes.
- ZP zona pellucid
- the ICSI needle or hatching needle pierce ZP at other positions, for example 5 o'clock position, the ovum or embryo would be deformed or damaged due to the interlace mechanical pressure ⁇ the holding force and pierce force are not at the same axis. So an operator has to face more difficulties and need much more time in micro-processes with the traditional micro-holding pipette.
- the ovum or embryo is held conveniently at a 45-past-8 o'clock to 7 o'clock position and the ZP is easily pierced or drilled at a 4 o'clock to 5 o'clock position by the opposite micropipette.
- Characteristics of the present invention are that: the holding end of the MICRO-HOLDING PIPETTE is an oblique opening, i.e. the surface of the opening of the holding end of the micropipette is at an acute angle with the ordinate axis of the holding end, which is less than 90° and preferably is from 25° to 85°.
- micropipettes or needles opposite to the MICRO-HOLDING PIPETTE can pierce or drill a ZP from the side not obverse surface of an ovum or embryo, the ovum or embryo do not deform due to the pressures, and it is beneficial to various micro-processes.
- the novel MICRO-HOLDING PIPETTE can significantly prevent damage to the embryo and improve the healthy growth of the embryo.
- Fig.3 and Fig.4 show that: the holding end of the MICRO-HOLDING PIPETTE '1' is an oblique opening '2', i.e. the surface of the opening of the holding end '5' of the micropipette is at an acute angle with the ordinate axis '6' of the holding end, which is less than 90° , and preferably is from 25° to 85°.
- Fig.5 shows that: an embryo '3' is held at an 8 o'clock position and the hatching needle '4' easily pierces a ZP '3' of embryo at a 5 o'clock position (Fig.6).
- both the present invention and the hatching needle are at the same pressure axis that ensures the needle easily pierces or drill the ZP without deformation or damaging of the embryo.
- the traditional MICRO-HOLDING PIPETTE does not have this advantage because the embryo has to be held at a 9 o'clock position.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Surgery (AREA)
- Reproductive Health (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Animal Behavior & Ethology (AREA)
- Molecular Biology (AREA)
- Medical Informatics (AREA)
- Heart & Thoracic Surgery (AREA)
- Biomedical Technology (AREA)
- Pregnancy & Childbirth (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Gynecology & Obstetrics (AREA)
- Transplantation (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2003/000363 WO2004100807A1 (fr) | 2003-05-19 | 2003-05-19 | Micro-pipette avec ouverture oblique |
Publications (1)
Publication Number | Publication Date |
---|---|
EP1629788A1 true EP1629788A1 (fr) | 2006-03-01 |
Family
ID=33438151
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03732175A Withdrawn EP1629788A1 (fr) | 2003-05-19 | 2003-05-19 | Micro-pipette avec ouverture oblique |
Country Status (5)
Country | Link |
---|---|
US (1) | US20060194305A1 (fr) |
EP (1) | EP1629788A1 (fr) |
CN (1) | CN100366226C (fr) |
AU (1) | AU2003242116A1 (fr) |
WO (1) | WO2004100807A1 (fr) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES1187335Y (es) * | 2017-06-21 | 2017-10-02 | Alcaide Francisco Vergara | Pipeta de sujecion de ovocitos en procedimientos de inyeccion espermatica intracitoplasmatica |
Family Cites Families (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3044015C2 (de) * | 1980-11-22 | 1984-03-29 | Uwe Dr.med. 5223 Harscheid Claussen | Verfahren und Einrichtung zur Präparation von in Teilung vorliegenden Zellen für die Chromosomenanalyse |
US4403617A (en) * | 1981-09-08 | 1983-09-13 | Waters Instruments, Inc. | Biopsy needle |
DE69330374T2 (de) * | 1992-11-10 | 2002-05-02 | Seikagaku Kogyo K.K., Tokio/Tokyo | Injektor und dessen anwendung |
US5472419A (en) * | 1994-01-31 | 1995-12-05 | Imagyn Medical, Inc. | Catheter and method for depositing reproductive material into the reproductive tract of a female |
US5968022A (en) * | 1995-04-28 | 1999-10-19 | Saito; Yoshikuni | Medical hollow needle and method of production |
DE69914029T2 (de) * | 1998-08-31 | 2004-11-18 | Sysmex Corp. | Probenansauger mit zwei zusammenarbeitenden Handhabungseinheiten |
NL1011522C2 (nl) * | 1999-03-10 | 2000-09-12 | Jozefus Elbertus Johan Berbers | Inrichting voor het overbrengen van een ovum vanuit een follikel. |
ATE443572T1 (de) * | 1999-05-14 | 2009-10-15 | Gen Probe Inc | Sammelvorrichtung mit probenentnahmevorrichtung |
JP2001190560A (ja) * | 2000-01-07 | 2001-07-17 | Nipro Corp | 採卵針およびそれを用いた洗浄吸引採卵装置 |
KR100706009B1 (ko) * | 2000-07-03 | 2007-04-11 | 도쿠타 쟈판 가부시키가이샤 | 의료용 베벨 바늘 |
US6511415B1 (en) * | 2000-11-03 | 2003-01-28 | Continental Plastic Corp. | Device for trans-cervical artificial insemination and embryo transfer |
CN1379977A (zh) * | 2001-04-10 | 2002-11-20 | 陆长富 | 一种构建哺乳动物克隆胚的方法及人类克隆胚的构建 |
JP2003125750A (ja) * | 2001-07-10 | 2003-05-07 | Narishige:Kk | マイクロピペット、インジェクション装置およびマイクロインジェクションの方法 |
-
2003
- 2003-05-19 WO PCT/CN2003/000363 patent/WO2004100807A1/fr not_active Application Discontinuation
- 2003-05-19 EP EP03732175A patent/EP1629788A1/fr not_active Withdrawn
- 2003-05-19 CN CNB038265974A patent/CN100366226C/zh not_active Expired - Fee Related
- 2003-05-19 AU AU2003242116A patent/AU2003242116A1/en not_active Abandoned
- 2003-05-19 US US10/565,655 patent/US20060194305A1/en not_active Abandoned
Non-Patent Citations (1)
Title |
---|
See references of WO2004100807A1 * |
Also Published As
Publication number | Publication date |
---|---|
CN100366226C (zh) | 2008-02-06 |
WO2004100807A1 (fr) | 2004-11-25 |
CN1787784A (zh) | 2006-06-14 |
US20060194305A1 (en) | 2006-08-31 |
AU2003242116A1 (en) | 2004-12-03 |
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Legal Events
Date | Code | Title | Description |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
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17P | Request for examination filed |
Effective date: 20051219 |
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AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR |
|
DAX | Request for extension of the european patent (deleted) | ||
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20071201 |