EP1622717A2 - Enrichissement de proteines et/ou de peptides phosphoryles base sur l'affinite - Google Patents

Enrichissement de proteines et/ou de peptides phosphoryles base sur l'affinite

Info

Publication number
EP1622717A2
EP1622717A2 EP04729634A EP04729634A EP1622717A2 EP 1622717 A2 EP1622717 A2 EP 1622717A2 EP 04729634 A EP04729634 A EP 04729634A EP 04729634 A EP04729634 A EP 04729634A EP 1622717 A2 EP1622717 A2 EP 1622717A2
Authority
EP
European Patent Office
Prior art keywords
proteins
phosphorylated peptides
substance
phosphorylated
affinity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP04729634A
Other languages
German (de)
English (en)
Inventor
Vukic Soskic
André SCHRATTENHOLZ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ProteoSys AG
Original Assignee
ProteoSys AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ProteoSys AG filed Critical ProteoSys AG
Publication of EP1622717A2 publication Critical patent/EP1622717A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3214—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the method for obtaining this coating or impregnating
    • B01J20/3217—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond
    • B01J20/3219—Resulting in a chemical bond between the coating or impregnating layer and the carrier, support or substrate, e.g. a covalent bond involving a particular spacer or linking group, e.g. for attaching an active group
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3246—Non-macromolecular compounds having a well defined chemical structure
    • B01J20/3248—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such
    • B01J20/3255—Non-macromolecular compounds having a well defined chemical structure the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30—Processes for preparing, regenerating, or reactivating
    • B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244—Non-macromolecular compounds
    • B01J20/3265—Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography

Definitions

  • the spacer is inclusive of the at least two groups 2,5-, 3,4- and / or 3,5-bis [(2,2'-dipicolylamino) methyl] benzene acid or the corresponding one Benzolklaremethylester.
  • the sample can also be further purified.
  • the use of samples without further purification is particularly preferred, since in this way all phosphorylated peptides and / or proteins of interest can be enriched and / or examined in more detail in this way, as is of particular interest in the field of proteome research.
  • the sample is first brought into contact with a substance, as has already been described, so that interactions between the substance and the phosphorylated peptides and / or proteins can form from the sample.
  • unbound material in particular non-phosphorylated peptides and / or proteins, is removed from the substance. This can be done in particular by washing with suitable buffer solutions.
  • V 2 is, for example, 2,2'-dipicolylamine.
  • TBS Tris-buffered saline
  • TBS / Tween 0.1% Tween 20 in TBS
  • BCIP / NBT bromochloroindolyl phosphate / nitro blue tetrazolium
  • Figure 4 shows the results of dot blot screening.
  • Dot 1 shows sample 1, dot 2 sample 2, dot 3 sample 3, etc.
  • the antibody solution was poured away. Without allowing the blot to dry, the blot was washed four times with 50 ml of TBS / Tween for 5 min. washed. This washing solution was poured off again and the solution with the second antibody was added to the blot.
  • Zn lysis buffer: 10 mM imidazole-HCl, 40 mM Na-MES, 2 ⁇ M Zn (S0 4 ) 2 , 0.5% Zwittergent 3-10, 0.5% CHAPS, 5 mM NaF, 1 mM Na 3 V0 4 , 0.2 mM Na pervanadate, 1x PIC, pH 5.5

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Pyridine Compounds (AREA)

Abstract

L'invention concerne une substance qui comprend un support solide relié à un espaceur par l'intermédiaire d'un lieur, cet espaceur comportant au moins deux groupes déterminés. Ladite substance peut être utilisée comme matière d'affinité pour enrichir et/ou isoler des protéines et/ou des peptides phosphorylés. Cette substance permet en particulier d'enrichir et/ou d'isoler des protéines et/ou des peptides phosphorylés sur tyrosine.
EP04729634A 2003-05-06 2004-04-27 Enrichissement de proteines et/ou de peptides phosphoryles base sur l'affinite Withdrawn EP1622717A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10321901A DE10321901A1 (de) 2003-05-06 2003-05-06 Affinitätsanreicherung von phosphorylierten Peptiden und/oder Proteinen
PCT/EP2004/004405 WO2004099233A2 (fr) 2003-05-06 2004-04-27 Enrichissement de proteines et/ou de peptides phosphoryles base sur l'affinite

Publications (1)

Publication Number Publication Date
EP1622717A2 true EP1622717A2 (fr) 2006-02-08

Family

ID=33394627

Family Applications (1)

Application Number Title Priority Date Filing Date
EP04729634A Withdrawn EP1622717A2 (fr) 2003-05-06 2004-04-27 Enrichissement de proteines et/ou de peptides phosphoryles base sur l'affinite

Country Status (7)

Country Link
US (1) US20070134648A1 (fr)
EP (1) EP1622717A2 (fr)
JP (1) JP2008500943A (fr)
AU (1) AU2004235906A1 (fr)
CA (1) CA2524522A1 (fr)
DE (1) DE10321901A1 (fr)
WO (1) WO2004099233A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009545317A (ja) * 2006-08-01 2009-12-24 アプライド バイオシステムズ， エルエルシー 分析物および核酸の検出
WO2014120794A1 (fr) * 2013-01-29 2014-08-07 Massachusetts Institute Of Technology Séparation magnétique à l'aide de nanoparticules
CN103447090B (zh) * 2013-07-23 2015-05-06 宁波大学 一种对甲基苯甲酸铀酰配合物光催化剂
JP6544571B2 (ja) * 2015-08-04 2019-07-17 国立大学法人山形大学 エタノールアミンリン酸センサ及びその製造方法
CN113607868B (zh) * 2021-06-15 2022-03-15 广东省农业科学院农业生物基因研究中心 一种用于磷酸化蛋白质组学的在线自动化分析装置和分析方法
CN117736257A (zh) * 2023-12-18 2024-03-22 嘉华药锐科技(北京)有限公司 一种酪氨酸磷酸化多肽富集方法及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE466534B (sv) * 1988-12-30 1992-03-02 Exploaterings Ab Tbf Adsorptionsmedel foer metalljoner, proteiner och andra oorganiska och organiska substanser
FR2768817B1 (fr) * 1997-09-19 1999-12-10 Cis Bio Int Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO2004099233A3 *

Also Published As

Publication number Publication date
DE10321901A1 (de) 2004-12-02
JP2008500943A (ja) 2008-01-17
CA2524522A1 (fr) 2004-11-18
WO2004099233A2 (fr) 2004-11-18
WO2004099233A3 (fr) 2005-01-27
US20070134648A1 (en) 2007-06-14
AU2004235906A1 (en) 2004-11-18

Similar Documents

Publication Publication Date Title
DE19926068C1 (de) Muteine des Bilin-Bindungsproteins
DE10113776A1 (de) Sequenziell angeordnete streptavidinbindende Module als Affinitätsanhängsel
WO2005111062A1 (fr) Procede et necessaire pour isoler des peptides phosphoryles
EP1622717A2 (fr) Enrichissement de proteines et/ou de peptides phosphoryles base sur l'affinite
WO2003074546A2 (fr) Peptide de liaison a la streptavidine
EP1015885B1 (fr) Epuration de substances provenant d'un echantillon biologique
EP2276777A2 (fr) Enrichissement sélectif de peptides modifiés sur le plan n-terminal à partir d'échantillons complexes
CN112898172B (zh) 可被羧肽酶酶解的双亲和功能团化合物的合成方法
Ross Identification of histidine phosphorylations in proteins using mass spectrometry and affinity‐based techniques
EP2268392B1 (fr) Procédé d'isolement de polypeptides
DE102005011579B4 (de) Affinitätsmarker zur Proteinreinigung, seine Herstellung und Verwendung sowie Verfahren zur Aufreinigung eines Proteins
EP2437618B1 (fr) Procédé de séparation de protéines végétales
DE19509881B4 (de) Ovoglycoprotein und dasselbe enthaltende Agens für chromatografische Aufspaltung
EP0306541B1 (fr) Procédé pour isoler l'hydrochlorure de daunorubicine de bouillons de fermentation
WO2009083252A2 (fr) Procédé d'enrichissement de phosphopeptides
US6143533A (en) Method for the preparation of N-glycolyl neuraminic acid from Cucumaria echinata
EP1361438A1 (fr) Analyse spectroscopique et spectrométrique de mélanges de peptides complexes assisté par phase solide
DE02747916T1 (de) Immunaffinitätsisolierung modifizierter peptide aus komplexen gemischen
DE1492114C (de) Verfahren zur sauren Extraktion und Gewinnung in gereinigter Form von Polypeptiden mit kallikrein- und trypsin hemmen der Wirkung aus tierischen Organen
WO2008046665A2 (fr) Procédé d'identification d'inhibiteurs de l'action de convertases sur l'urotensine ii
EP1504267A1 (fr) Analyse spectroscopique et spectrometrique de melanges biologiques complexes assistee par des phases solides
DE10228857A1 (de) Tetraetherlipide mit kleinen Kopfgruppen, deren Herstellung und Verwendung
DD276692A1 (de) Verfahren zur gewinnung rekombinanter proteine aus gram-negativen mikroorganismen
DE10131181A1 (de) Verfahren zur Herstellung nukleotidbindender Fusionsproteine, deren Aufreinigung und Kopplung an Oberflächen
DE4401135A1 (de) Verbindungen mit Träger- und Substanz-Bindungsstellen

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20051203

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PL PT RO SE SI SK TR

DAX Request for extension of the european patent (deleted)
RIN1 Information on inventor provided before grant (corrected)

Inventor name: SOSKIC, VUKIC

Inventor name: SCHRATTENHOLZ, ANDRE

17Q First examination report despatched

Effective date: 20090827

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20091031