EP1606624A1 - Technique de detection d'une forme pathogene de proteine prionique - Google Patents

Technique de detection d'une forme pathogene de proteine prionique

Info

Publication number
EP1606624A1
EP1606624A1 EP03816467A EP03816467A EP1606624A1 EP 1606624 A1 EP1606624 A1 EP 1606624A1 EP 03816467 A EP03816467 A EP 03816467A EP 03816467 A EP03816467 A EP 03816467A EP 1606624 A1 EP1606624 A1 EP 1606624A1
Authority
EP
European Patent Office
Prior art keywords
prion protein
container
pathogenic
binding
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03816467A
Other languages
German (de)
English (en)
Inventor
Josephus Wilhelmus Alphonsus Maria Van Oers
Teun Jan Karel Van Der Vorst
Cornelis Erik Hack
Franciscus Antonius Cornelis Van Engelenburg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stichting Sanquin Bloedvoorziening
Original Assignee
Stichting Sanquin Bloedvoorziening
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stichting Sanquin Bloedvoorziening filed Critical Stichting Sanquin Bloedvoorziening
Priority to EP03816467A priority Critical patent/EP1606624A1/fr
Publication of EP1606624A1 publication Critical patent/EP1606624A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G01N33/6896—Neurological disorders, e.g. Alzheimer's disease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/28—Neurological disorders
    • G01N2800/2814—Dementia; Cognitive disorders
    • G01N2800/2828—Prion diseases

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

La présente invention concerne une technique de détection d'une forme pathogène de protéine prionique dans un échantillon qui consiste à prendre un récipient et, prétraiter ce récipient de façon à déposer sur une surface de celui-ci un revêtement d'un dérivé de cellulose capable de favoriser la liaison d'une protéine prionique pathogène avec cette surface de récipient sur la liaison de protéine prionique cellulaire, à incuber l'échantillon dans ce récipient de façon à lier toute protéine prionique pathogène présente dans cet échantillon à la surface de ce récipient, à marquer cette protéine prionique immobilisée, le cas échéant, avec un agent de marquage approprié utilisant un anticorps de prion capable de se lier à la protéine prionique pathogène et é détecter la présence d'agent de marquage fixé à la surface du récipient. Revêtir une surface de puits de plaque de microtitration avec de la nitrocellulose permet d'effectuer un test ELISA destiné à quantifier des prions pathogènes dans un échantillon suspect, en particulier après la digestion enzymatique de cet échantillon avec une enzyme de type protéinase K, en renforçant la liaison de prions pathogène et/ou en réduisant la liaison de prions cellulaires (non pathogènes).
EP03816467A 2003-03-25 2003-10-30 Technique de detection d'une forme pathogene de proteine prionique Withdrawn EP1606624A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP03816467A EP1606624A1 (fr) 2003-03-25 2003-10-30 Technique de detection d'une forme pathogene de proteine prionique

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP03075881A EP1462804A1 (fr) 2003-03-25 2003-03-25 Méthode de détection de la forme pathogene de protéines prion
EP03075881 2003-03-25
PCT/NL2003/000741 WO2004086053A1 (fr) 2003-03-25 2003-10-30 Technique de detection d'une forme pathogene de proteine prionique
EP03816467A EP1606624A1 (fr) 2003-03-25 2003-10-30 Technique de detection d'une forme pathogene de proteine prionique

Publications (1)

Publication Number Publication Date
EP1606624A1 true EP1606624A1 (fr) 2005-12-21

Family

ID=32798961

Family Applications (2)

Application Number Title Priority Date Filing Date
EP03075881A Withdrawn EP1462804A1 (fr) 2003-03-25 2003-03-25 Méthode de détection de la forme pathogene de protéines prion
EP03816467A Withdrawn EP1606624A1 (fr) 2003-03-25 2003-10-30 Technique de detection d'une forme pathogene de proteine prionique

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP03075881A Withdrawn EP1462804A1 (fr) 2003-03-25 2003-03-25 Méthode de détection de la forme pathogene de protéines prion

Country Status (6)

Country Link
EP (2) EP1462804A1 (fr)
JP (1) JP2006521534A (fr)
CN (1) CN1820202A (fr)
AU (1) AU2003276778A1 (fr)
CA (1) CA2520487A1 (fr)
WO (1) WO2004086053A1 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103336124B (zh) * 2012-12-11 2015-07-15 武汉工业学院 一种检测朊病毒蛋白(PrPSC)的方法和试剂盒
CN107190050B (zh) * 2016-03-14 2020-10-27 华东理工大学 一种hrp活性测定及h2o2浓度检测的试剂盒及其应用
CN107884399B (zh) * 2017-11-08 2020-04-28 北京化工大学 一种嘌呤类化合物修饰纳米金材料及其试剂盒
CN108333174A (zh) * 2018-01-17 2018-07-27 大连博融新材料有限公司 一种可实现钒溶液浓度在线检测的分析方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221614B1 (en) * 1997-02-21 2001-04-24 The Regents Of The University Of California Removal of prions from blood, plasma and other liquids
US20020058031A1 (en) * 2000-09-19 2002-05-16 Tung Hsiaoho Edward Methods for preparing diagnostic reagents using antibody preparation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3524451A1 (de) * 1985-07-09 1987-03-12 Behringwerke Ag Mit ueberzuegen versehene formkoerper zur bindung von bioaffinen substanzen, verfahren zu ihrer herstellung sowie ihre verwendung
AU2580900A (en) * 1999-02-11 2000-08-29 Id-Lelystad, Instituut Voor Dierhouderij En Diergezondheid B.V. Prion test
IES20010042A2 (en) * 2001-01-19 2002-12-11 Enfer Technology Ltd Test for transmissible spongiform encephalopathies

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6221614B1 (en) * 1997-02-21 2001-04-24 The Regents Of The University Of California Removal of prions from blood, plasma and other liquids
US20020058031A1 (en) * 2000-09-19 2002-05-16 Tung Hsiaoho Edward Methods for preparing diagnostic reagents using antibody preparation

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2004086053A1 *

Also Published As

Publication number Publication date
CA2520487A1 (fr) 2004-10-07
CN1820202A (zh) 2006-08-16
AU2003276778A1 (en) 2004-10-18
JP2006521534A (ja) 2006-09-21
EP1462804A1 (fr) 2004-09-29
WO2004086053A1 (fr) 2004-10-07

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