EP1578951A4 - Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge - Google Patents

Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge

Info

Publication number
EP1578951A4
EP1578951A4 EP03809647A EP03809647A EP1578951A4 EP 1578951 A4 EP1578951 A4 EP 1578951A4 EP 03809647 A EP03809647 A EP 03809647A EP 03809647 A EP03809647 A EP 03809647A EP 1578951 A4 EP1578951 A4 EP 1578951A4
Authority
EP
European Patent Office
Prior art keywords
dna polymerases
analog detection
base analog
reduced base
detection activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03809647A
Other languages
English (en)
French (fr)
Other versions
EP1578951A2 (de
Inventor
Joseph A Sorge
Holly H Hogrefe
Madhushree Ghosh
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stratagene California
Original Assignee
Stratagene California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/280,962 external-priority patent/US20040081965A1/en
Priority claimed from US10/298,680 external-priority patent/US20040091873A1/en
Priority claimed from US10/408,601 external-priority patent/US20040086890A1/en
Application filed by Stratagene California filed Critical Stratagene California
Publication of EP1578951A2 publication Critical patent/EP1578951A2/de
Publication of EP1578951A4 publication Critical patent/EP1578951A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP03809647A 2002-10-25 2003-10-24 Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge Withdrawn EP1578951A4 (de)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US408601 1982-08-16
US280962 2002-10-25
US10/280,962 US20040081965A1 (en) 2002-10-25 2002-10-25 DNA polymerases with reduced base analog detection activity
US298680 2002-11-18
US10/298,680 US20040091873A1 (en) 2002-10-25 2002-11-18 DNA polymerases with reduced base analog detection activity
US10/408,601 US20040086890A1 (en) 2002-10-25 2003-04-07 DNA polymerases with reduced base analog detection activity
PCT/US2003/033997 WO2004038007A2 (en) 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity

Publications (2)

Publication Number Publication Date
EP1578951A2 EP1578951A2 (de) 2005-09-28
EP1578951A4 true EP1578951A4 (de) 2005-11-16

Family

ID=46205001

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03809647A Withdrawn EP1578951A4 (de) 2002-10-25 2003-10-24 Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge

Country Status (5)

Country Link
EP (1) EP1578951A4 (de)
JP (1) JP2006507012A (de)
AU (1) AU2003301590A1 (de)
CA (1) CA2503038A1 (de)
WO (1) WO2004038007A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
KR100844358B1 (ko) * 2006-10-02 2008-07-07 한국해양연구원 돌연변이 ｄｎａ 중합효소들 및 그의 유전자들
JP5258512B2 (ja) * 2008-10-30 2013-08-07 株式会社日立製作所 エキソヌクレアーゼ活性増強ｄｎａポリメラーゼ変異体
CN102272319B (zh) * 2009-01-08 2014-08-27 伯乐实验室公司 用于提高核酸扩增反应效率的方法和组合物
JP6478446B2 (ja) * 2012-09-28 2019-03-06 東洋紡株式会社 改変された耐熱性ｄｎａポリメラーゼ
JP6428997B2 (ja) * 2013-08-06 2018-11-28 東洋紡株式会社 核酸増幅法
JP6493209B2 (ja) * 2013-08-06 2019-04-03 東洋紡株式会社 核酸増幅法
CN114517189A (zh) 2014-11-14 2022-05-20 亿明达股份有限公司 聚合酶
JP6968536B2 (ja) * 2014-11-28 2021-11-17 東洋紡株式会社 核酸増幅試薬
WO2016104272A1 (ja) * 2014-12-25 2016-06-30 東洋紡株式会社 Ｐｃｒ方法
CN114891761B (zh) * 2022-03-25 2024-01-12 上海威高医疗技术发展有限公司 Tth DNA聚合酶突变体及其应用
CN116410952A (zh) * 2022-12-28 2023-07-11 郑州玛特瑞斯生物科技有限公司 突变型Taq DNA聚合酶、编码基因、重组表达载体、重组菌及其应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001092501A1 (en) * 2000-05-26 2001-12-06 Mj Bioworks, Inc. Improved nucleic acid modifying enzymes
WO2003060144A2 (en) * 2001-12-21 2003-07-24 Stratagene High fidelity dna polymerase compositions and uses therefor
WO2003089637A1 (en) * 2002-04-17 2003-10-30 The University Of Newcastle_Upon Tyne Mutation of dna polymerases from archaeobacteria

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60027040T2 (de) * 1999-10-29 2006-11-23 Stratagene California, La Jolla Zusammensetzungen und methoden zur verwendung von dna polymerasen
EP1232270A1 (de) * 1999-11-23 2002-08-21 Amersham Biosciences Corp. Verbesserter dideoxynucleotidtriphosphatverbrauch durch hyperthermophile dna polymerase aus dem archaebakterium i(pyrococcus furiosus)
JP2001269188A (ja) * 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Ｐｃｒにおける性能が向上したｂ型ｄｎａポリメラーゼ変異体

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001092501A1 (en) * 2000-05-26 2001-12-06 Mj Bioworks, Inc. Improved nucleic acid modifying enzymes
WO2003060144A2 (en) * 2001-12-21 2003-07-24 Stratagene High fidelity dna polymerase compositions and uses therefor
WO2003089637A1 (en) * 2002-04-17 2003-10-30 The University Of Newcastle_Upon Tyne Mutation of dna polymerases from archaeobacteria

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CONNOLLY B A ET AL: "Uracil recognition by archaeal family B DNA polymerases.", BIOCHEMICAL SOCIETY TRANSACTIONS. ENGLAND JUN 2003, vol. 31, no. Pt 3, June 2003 (2003-06-01), pages 699 - 702, XP009015138, ISSN: 0300-5127 *
FOGG MARK J ET AL: "Structural basis for uracil recognition by archaeal family B DNA polymerases.", NATURE STRUCTURAL BIOLOGY. UNITED STATES DEC 2002, vol. 9, no. 12, December 2002 (2002-12-01), pages 922 - 927, XP002250141, ISSN: 1072-8368 *
GARDNER ANDREW F ET AL: "Determinants of nucleotide sugar recognition in an archaeon DNA polymerase", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 12, 15 June 1999 (1999-06-15), pages 2545 - 2553, XP002163101, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
EP1578951A2 (de) 2005-09-28
JP2006507012A (ja) 2006-03-02
WO2004038007A3 (en) 2005-02-10
AU2003301590A1 (en) 2004-05-13
WO2004038007A2 (en) 2004-05-06
CA2503038A1 (en) 2004-05-06

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Inventor name: GHOSH, MADHUSHREE

Inventor name: HOGREFE, HOLLY, H.

Inventor name: SORGE, JOSEPH, A.

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