EP1578951A4 - Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge - Google Patents
Dna-polymerase mit reduzierter nachweisaktivitätfür basenanalogeInfo
- Publication number
- EP1578951A4 EP1578951A4 EP03809647A EP03809647A EP1578951A4 EP 1578951 A4 EP1578951 A4 EP 1578951A4 EP 03809647 A EP03809647 A EP 03809647A EP 03809647 A EP03809647 A EP 03809647A EP 1578951 A4 EP1578951 A4 EP 1578951A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- dna polymerases
- analog detection
- base analog
- reduced base
- detection activity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US408601 | 1982-08-16 | ||
US10/280,962 US20040081965A1 (en) | 2002-10-25 | 2002-10-25 | DNA polymerases with reduced base analog detection activity |
US280962 | 2002-10-25 | ||
US10/298,680 US20040091873A1 (en) | 2002-10-25 | 2002-11-18 | DNA polymerases with reduced base analog detection activity |
US298680 | 2002-11-18 | ||
US10/408,601 US20040086890A1 (en) | 2002-10-25 | 2003-04-07 | DNA polymerases with reduced base analog detection activity |
PCT/US2003/033997 WO2004038007A2 (en) | 2002-10-25 | 2003-10-24 | Dna polymerases with reduced base analog detection activity |
Publications (2)
Publication Number | Publication Date |
---|---|
EP1578951A2 EP1578951A2 (de) | 2005-09-28 |
EP1578951A4 true EP1578951A4 (de) | 2005-11-16 |
Family
ID=46205001
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP03809647A Withdrawn EP1578951A4 (de) | 2002-10-25 | 2003-10-24 | Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1578951A4 (de) |
JP (1) | JP2006507012A (de) |
AU (1) | AU2003301590A1 (de) |
CA (1) | CA2503038A1 (de) |
WO (1) | WO2004038007A2 (de) |
Families Citing this family (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7960157B2 (en) | 2002-12-20 | 2011-06-14 | Agilent Technologies, Inc. | DNA polymerase blends and uses thereof |
KR100844358B1 (ko) * | 2006-10-02 | 2008-07-07 | 한국해양연구원 | 돌연변이 dna 중합효소들 및 그의 유전자들 |
JP5258512B2 (ja) * | 2008-10-30 | 2013-08-07 | 株式会社日立製作所 | エキソヌクレアーゼ活性増強dnaポリメラーゼ変異体 |
CA2749093C (en) * | 2009-01-08 | 2019-03-26 | Bio-Rad Laboratories, Inc. | Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions |
JP6478446B2 (ja) | 2012-09-28 | 2019-03-06 | 東洋紡株式会社 | 改変された耐熱性dnaポリメラーゼ |
WO2015019658A1 (ja) * | 2013-08-06 | 2015-02-12 | 東洋紡株式会社 | 核酸増幅法 |
JP6493209B2 (ja) * | 2013-08-06 | 2019-04-03 | 東洋紡株式会社 | 核酸増幅法 |
WO2016077795A1 (en) * | 2014-11-14 | 2016-05-19 | Illumina, Inc. | Polymerases |
WO2016084879A1 (ja) * | 2014-11-28 | 2016-06-02 | 東洋紡株式会社 | 核酸増幅試薬 |
WO2016104272A1 (ja) * | 2014-12-25 | 2016-06-30 | 東洋紡株式会社 | Pcr方法 |
GB201802744D0 (en) * | 2018-02-20 | 2018-04-04 | Longas Tech Pty Ltd | Method |
CN114891761B (zh) * | 2022-03-25 | 2024-01-12 | 上海威高医疗技术发展有限公司 | Tth DNA聚合酶突变体及其应用 |
CN116410952A (zh) * | 2022-12-28 | 2023-07-11 | 郑州玛特瑞斯生物科技有限公司 | 突变型Taq DNA聚合酶、编码基因、重组表达载体、重组菌及其应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001092501A1 (en) * | 2000-05-26 | 2001-12-06 | Mj Bioworks, Inc. | Improved nucleic acid modifying enzymes |
WO2003060144A2 (en) * | 2001-12-21 | 2003-07-24 | Stratagene | High fidelity dna polymerase compositions and uses therefor |
WO2003089637A1 (en) * | 2002-04-17 | 2003-10-30 | The University Of Newcastle_Upon Tyne | Mutation of dna polymerases from archaeobacteria |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4949585B2 (ja) * | 1999-10-29 | 2012-06-13 | アジレント・テクノロジーズ・インク | Dnaポリメラーゼを使用した組成物および方法 |
AU1491401A (en) * | 1999-11-23 | 2001-06-04 | Amersham Pharmacia Biotech Inc. | Improving dideoxynucleotide-triphosphate utilization by the hyper-thermophilic dna polymerase from the archaeon pyrococcus furiosus |
JP2001269188A (ja) * | 2000-03-11 | 2001-10-02 | Roche Diagnostics Gmbh | Pcrにおける性能が向上したb型dnaポリメラーゼ変異体 |
-
2003
- 2003-10-24 EP EP03809647A patent/EP1578951A4/de not_active Withdrawn
- 2003-10-24 WO PCT/US2003/033997 patent/WO2004038007A2/en active Application Filing
- 2003-10-24 JP JP2005501697A patent/JP2006507012A/ja active Pending
- 2003-10-24 CA CA002503038A patent/CA2503038A1/en not_active Abandoned
- 2003-10-24 AU AU2003301590A patent/AU2003301590A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001092501A1 (en) * | 2000-05-26 | 2001-12-06 | Mj Bioworks, Inc. | Improved nucleic acid modifying enzymes |
WO2003060144A2 (en) * | 2001-12-21 | 2003-07-24 | Stratagene | High fidelity dna polymerase compositions and uses therefor |
WO2003089637A1 (en) * | 2002-04-17 | 2003-10-30 | The University Of Newcastle_Upon Tyne | Mutation of dna polymerases from archaeobacteria |
Non-Patent Citations (3)
Title |
---|
CONNOLLY B A ET AL: "Uracil recognition by archaeal family B DNA polymerases.", BIOCHEMICAL SOCIETY TRANSACTIONS. ENGLAND JUN 2003, vol. 31, no. Pt 3, June 2003 (2003-06-01), pages 699 - 702, XP009015138, ISSN: 0300-5127 * |
FOGG MARK J ET AL: "Structural basis for uracil recognition by archaeal family B DNA polymerases.", NATURE STRUCTURAL BIOLOGY. UNITED STATES DEC 2002, vol. 9, no. 12, December 2002 (2002-12-01), pages 922 - 927, XP002250141, ISSN: 1072-8368 * |
GARDNER ANDREW F ET AL: "Determinants of nucleotide sugar recognition in an archaeon DNA polymerase", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 27, no. 12, 15 June 1999 (1999-06-15), pages 2545 - 2553, XP002163101, ISSN: 0305-1048 * |
Also Published As
Publication number | Publication date |
---|---|
WO2004038007A2 (en) | 2004-05-06 |
EP1578951A2 (de) | 2005-09-28 |
WO2004038007A3 (en) | 2005-02-10 |
AU2003301590A1 (en) | 2004-05-13 |
CA2503038A1 (en) | 2004-05-06 |
JP2006507012A (ja) | 2006-03-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2003296896A8 (en) | Dna polymerase mutants with reverse transcriptase activity | |
AU2003256703A8 (en) | Biosensor arrays and methods | |
EP1731525A4 (de) | Nukleotidderivate und dna-mikroarray | |
EP1506310A4 (de) | Biosensor | |
AU2003299817A8 (en) | Genetic polymorphisms associated with stenosis, methods of detection and uses thereof | |
AU2003256787A8 (en) | Petal-array support for use with microplates | |
IL174572A0 (en) | Refractory period tracking and arrhythmia detection | |
EP1652855A4 (de) | Polypeptide mit hirnablagernder wirkung und deren verwendung | |
HK1095742A1 (en) | 3-cyano-quinoline derivatives with antiproliferative activity | |
EP1816130A4 (de) | Neues nukleosid- oder nukleotidderivat und verwendung davon | |
EP1578951A4 (de) | Dna-polymerase mit reduzierter nachweisaktivitätfür basenanaloge | |
AU2003290432A8 (en) | T cell activating gene | |
GB0427709D0 (en) | Waveform analysis | |
AU2003273778A8 (en) | Oligonucleotide analogues for detecting and analyzing nucleic acids | |
GB0225833D0 (en) | Nucleic acid ligands and uses therefor | |
AU2003290722A8 (en) | Methods and compositions for detecting telomerase activity | |
AU2003254298A8 (en) | Species specific dna detection | |
IL166029A0 (en) | Storing and detecting nucleic acid administered toa solid carrier | |
AU2003296087A1 (en) | Nucleotide derivatives and dna microarray | |
AU2003293382A8 (en) | Methods for identifying the activity of gene products | |
HK1060178A1 (en) | Biosensor | |
AU2003294705A8 (en) | Ntsm gene | |
EP1590432A4 (de) | Dna-zusammensetzung und ihre verwendung | |
AU2003294287A8 (en) | A continuous-read assay for the detection of de novo hcv rna polymerase activity | |
GB0205058D0 (en) | Support and dedicated pen assembly |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 20050524 |
|
AK | Designated contracting states |
Kind code of ref document: A2 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR |
|
AX | Request for extension of the european patent |
Extension state: AL LT LV MK |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: 7C 07H 21/04 B Ipc: 7C 12P 19/34 B Ipc: 7C 12Q 1/68 B Ipc: 7C 12N 9/00 B Ipc: 7C 12N 9/12 A |
|
A4 | Supplementary search report drawn up and despatched |
Effective date: 20050930 |
|
RIC1 | Information provided on ipc code assigned before grant |
Ipc: 7C 12N 15/03 B Ipc: 7C 12N 9/12 A |
|
DAX | Request for extension of the european patent (deleted) | ||
RIN1 | Information on inventor provided before grant (corrected) |
Inventor name: GHOSH, MADHUSHREE Inventor name: HOGREFE, HOLLY, H. Inventor name: SORGE, JOSEPH, A. |
|
17Q | First examination report despatched |
Effective date: 20081229 |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 20110729 |