WO2004038007A3 - Dna polymerases with reduced base analog detection activity - Google Patents

Dna polymerases with reduced base analog detection activity Download PDF

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Publication number
WO2004038007A3
WO2004038007A3 PCT/US2003/033997 US0333997W WO2004038007A3 WO 2004038007 A3 WO2004038007 A3 WO 2004038007A3 US 0333997 W US0333997 W US 0333997W WO 2004038007 A3 WO2004038007 A3 WO 2004038007A3
Authority
WO
WIPO (PCT)
Prior art keywords
analog detection
base analog
reduced base
detection activity
dna polymerases
Prior art date
Application number
PCT/US2003/033997
Other languages
French (fr)
Other versions
WO2004038007A2 (en
Inventor
Joseph A Sorge
Holly H Hogrefe
Madhushree Ghosh
Original Assignee
Stratagene Inc
Joseph A Sorge
Holly H Hogrefe
Madhushree Ghosh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/280,962 external-priority patent/US20040081965A1/en
Priority claimed from US10/298,680 external-priority patent/US20040091873A1/en
Priority claimed from US10/408,601 external-priority patent/US20040086890A1/en
Application filed by Stratagene Inc, Joseph A Sorge, Holly H Hogrefe, Madhushree Ghosh filed Critical Stratagene Inc
Priority to EP03809647A priority Critical patent/EP1578951A4/en
Priority to JP2005501697A priority patent/JP2006507012A/en
Priority to AU2003301590A priority patent/AU2003301590A1/en
Priority to CA002503038A priority patent/CA2503038A1/en
Publication of WO2004038007A2 publication Critical patent/WO2004038007A2/en
Publication of WO2004038007A3 publication Critical patent/WO2004038007A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3'-5' exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
PCT/US2003/033997 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity WO2004038007A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03809647A EP1578951A4 (en) 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity
JP2005501697A JP2006507012A (en) 2002-10-25 2003-10-24 DNA polymerase with reduced base analog detection activity
AU2003301590A AU2003301590A1 (en) 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity
CA002503038A CA2503038A1 (en) 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US10/280,962 2002-10-25
US10/280,962 US20040081965A1 (en) 2002-10-25 2002-10-25 DNA polymerases with reduced base analog detection activity
US10/298,680 2002-11-18
US10/298,680 US20040091873A1 (en) 2002-10-25 2002-11-18 DNA polymerases with reduced base analog detection activity
US10/408,601 US20040086890A1 (en) 2002-10-25 2003-04-07 DNA polymerases with reduced base analog detection activity
US10/408,601 2003-04-07

Publications (2)

Publication Number Publication Date
WO2004038007A2 WO2004038007A2 (en) 2004-05-06
WO2004038007A3 true WO2004038007A3 (en) 2005-02-10

Family

ID=46205001

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/033997 WO2004038007A2 (en) 2002-10-25 2003-10-24 Dna polymerases with reduced base analog detection activity

Country Status (5)

Country Link
EP (1) EP1578951A4 (en)
JP (1) JP2006507012A (en)
AU (1) AU2003301590A1 (en)
CA (1) CA2503038A1 (en)
WO (1) WO2004038007A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108064278B (en) * 2014-11-14 2022-02-08 亿明达股份有限公司 Polymerase enzyme

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7960157B2 (en) 2002-12-20 2011-06-14 Agilent Technologies, Inc. DNA polymerase blends and uses thereof
KR100844358B1 (en) * 2006-10-02 2008-07-07 한국해양연구원 Mutant DNA Polymerases and Their Genes
JP5258512B2 (en) * 2008-10-30 2013-08-07 株式会社日立製作所 DNA polymerase mutant with enhanced exonuclease activity
CA2749093C (en) * 2009-01-08 2019-03-26 Bio-Rad Laboratories, Inc. Exonuclease deficient hybrid polymerase and uses thereof in amplification reactions
JP6478446B2 (en) 2012-09-28 2019-03-06 東洋紡株式会社 Modified thermostable DNA polymerase
WO2015019658A1 (en) * 2013-08-06 2015-02-12 東洋紡株式会社 Nucleic acid amplification method
JP6493209B2 (en) * 2013-08-06 2019-04-03 東洋紡株式会社 Nucleic acid amplification method
WO2016084879A1 (en) * 2014-11-28 2016-06-02 東洋紡株式会社 Nucleic acid amplification reagent
WO2016104272A1 (en) * 2014-12-25 2016-06-30 東洋紡株式会社 Pcr method
GB201802744D0 (en) * 2018-02-20 2018-04-04 Longas Tech Pty Ltd Method
CN114891761B (en) * 2022-03-25 2024-01-12 上海威高医疗技术发展有限公司 Tth DNA polymerase mutant and application thereof
CN116410952A (en) * 2022-12-28 2023-07-11 郑州玛特瑞斯生物科技有限公司 Mutant Taq DNA polymerase, coding gene, recombinant expression vector, recombinant bacterium and application thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6333183B1 (en) * 1999-11-23 2001-12-25 Amersham Pharmacia Biotech, Inc. Dideoxynucleotide-triphosphate utilization by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4949585B2 (en) * 1999-10-29 2012-06-13 アジレント・テクノロジーズ・インク Compositions and methods using DNA polymerases
JP2001269188A (en) * 2000-03-11 2001-10-02 Roche Diagnostics Gmbh Dna polymerase b variant exhibiting enhanced performance in pcr
US6627424B1 (en) * 2000-05-26 2003-09-30 Mj Bioworks, Inc. Nucleic acid modifying enzymes
US7932070B2 (en) * 2001-12-21 2011-04-26 Agilent Technologies, Inc. High fidelity DNA polymerase compositions and uses therefor
GB0208768D0 (en) * 2002-04-17 2002-05-29 Univ Newcastle DNA polymerases

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6333183B1 (en) * 1999-11-23 2001-12-25 Amersham Pharmacia Biotech, Inc. Dideoxynucleotide-triphosphate utilization by the hyper-thermophilic DNA polymerase from the archaeon Pyrococcus furiosus

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
EVANS ET AL.: "Improving dideoxynucleotide-triphosphate utilisation by the hyperthermophilic DNA polymerase from the archaeon Pyrococcus furiosus", NUCLEIC ACIDS RESEARCH, vol. 28, no. 5, March 2000 (2000-03-01), pages 1059 - 1066, XP002164138 *
See also references of EP1578951A4 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108064278B (en) * 2014-11-14 2022-02-08 亿明达股份有限公司 Polymerase enzyme

Also Published As

Publication number Publication date
WO2004038007A2 (en) 2004-05-06
EP1578951A2 (en) 2005-09-28
EP1578951A4 (en) 2005-11-16
AU2003301590A1 (en) 2004-05-13
CA2503038A1 (en) 2004-05-06
JP2006507012A (en) 2006-03-02

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