EP1556476A2 - Procede et dispositif pour manipuler des echantillons - Google Patents

Procede et dispositif pour manipuler des echantillons

Info

Publication number
EP1556476A2
EP1556476A2 EP03769021A EP03769021A EP1556476A2 EP 1556476 A2 EP1556476 A2 EP 1556476A2 EP 03769021 A EP03769021 A EP 03769021A EP 03769021 A EP03769021 A EP 03769021A EP 1556476 A2 EP1556476 A2 EP 1556476A2
Authority
EP
European Patent Office
Prior art keywords
images
samples
preparations
markings
preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP03769021A
Other languages
German (de)
English (en)
Inventor
Guntram Schnetz
Heinz Redl
Kurt Zatloukal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oridis Biomed Forschungs- und Entwicklungs GmbH
Original Assignee
Oridis Biomed Forschungs- und Entwicklungs GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oridis Biomed Forschungs- und Entwicklungs GmbH filed Critical Oridis Biomed Forschungs- und Entwicklungs GmbH
Publication of EP1556476A2 publication Critical patent/EP1556476A2/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
    • G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/32—Micromanipulators structurally combined with microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
    • G01N2001/2873—Cutting or cleaving
    • G01N2001/288—Filter punches

Definitions

  • the invention relates to a method for manipulation with samples, in particular tissue samples, samples being cut out from preparations, in particular prepared tissue parts, with the aid of needles at defined positions, which samples are introduced into holes which have been punched out in sample carriers.
  • the invention further relates to a device for manipulating samples, in particular tissue samples, with at least one needle for piercing samples from preparations, in particular prepared tissue parts at defined positions, and a control device for controlling the needle.
  • preparations includes in particular human or animal tissue parts. However, other substances or substances, such as e.g. embedded cell or bacterial suspensions, but also plants or parts of plants are examined and their samples manipulated.
  • tissue samples are often removed from human and animal organs and, after a series of preparation and processing steps, subjected to various examinations, for example in order to identify diseases or tissue changes or to be able to assess the course of therapy.
  • the removed tissue is usually embedded in paraffin, plastic or another comparable material and one or more targeted samples are cut out from this embedded tissue part.
  • cylindrical tissue samples are cut out with needles. These punched-out tissue samples are then introduced into correspondingly large holes also punched out with the aid of needles in a sample holder.
  • the sample carrier is usually made of paraffin, plastic or a similar material.
  • thermoplastic materials for embedding preparations in particular tissue parts, but also for introducing samples are known which are gel-like at room temperature and solidify at low temperature, for example at -10 °. Such materials can be used in particular to produce frozen samples. Needles are used to prick the holes in the sample carrier, the outer diameter of which essentially corresponds to the diameter corresponds to those needles with which the tissue samples are cut out of the tissue parts. The cut out tissue sample thus fits exactly into the prefabricated hole in the sample holder. In this way, so-called tissue arrays or microarrays are produced which contain a large number of tissue samples arranged next to one another.
  • tissue samples are usually made from the tissue sample arrangements produced in this way, which sections are then subjected to histological or pathological examinations.
  • tissue samples can be arranged on sample carriers, which have a size of 3 x 4 cm, for example.
  • the resulting number of individual samples, which result after the cuts have been made and must be evaluated, is correspondingly high.
  • the manipulation with the tissue samples should take place as quickly and automatically as possible.
  • devices for manipulating tissue samples were created, with the aid of which such tissue arrangements can be produced as quickly as possible and with the highest possible accuracy.
  • US Pat. No. 6,103,518 A describes a device for manipulating tissue samples of the type in question, in which holes are pierced in sample carriers with a needle and tissue samples are cut out from prepared tissue parts with a further needle, which tissue samples are introduced into the holes in the sample carriers which have been cleared become.
  • tissue samples are introduced into the holes in the sample carriers which have been cleared become.
  • Those places on the tissue parts where tissue samples are to be cut out are usually selected manually, which considerably slows down the manipulation process.
  • the selection of those points on the tissue parts at which tissue samples are cut out is usually carried out only under visual control by medical specialists. Tissue sections of the tissue parts can be used to support the selection of the cutting positions.
  • DE 198 15 400 AI relates to a device and a method for taking samples from polymeric carrier materials, in which samples can be cut out one after the other with a large number of separating tools, for example lancing capillaries, and then simultaneously deposited on a target substrate.
  • the device enables the same early processing of a large number of samples.
  • the combination of the sample recording device with an image recording system is described, by means of which automation and acceleration of the sample recording process is possible.
  • the selection of the desired lancing positions can be supported by the image of the surface of the preparation or the like from which the samples are stuck.
  • the aim of the present invention is therefore to create a method for manipulating samples, in particular tissue samples of the type specified above, which enables the desired piercing positions to be selected as quickly as possible and can be carried out as simply and quickly as possible.
  • the method according to the invention is intended to be able to produce samples which are of the highest possible quality and specificity.
  • Another object of the present invention is to provide a device for manipulation with samples, in particular tissue samples of the type specified, which permits the simplest possible selection and definition of the desired cutting positions on the specimens and which permits automatic or partially automatic manipulation.
  • the device should be constructed as simply and inexpensively as possible and should be as maintenance-free as possible.
  • the device is said to be suitable for manipulating a large number of samples. Disadvantages of the prior art should be avoided or at least reduced.
  • the first object according to the invention is achieved in that at least one digital, microscopic image of a section of a preparation is overlaid with an image of the surface of this preparation, and that markings are set on the overlaid image which define the desired positions at which the samples automatically cut out and inserted into the holes of the sample holder.
  • the superimposition of at least one digital, microscopic sectional image of the preparation Facilitates the selection of the optimal cutting positions on the specimen and thus increases the quality of the resulting samples and consequently also the quality of the diagnosis or the examination result.
  • markings at the desired cut-out positions the samples do not have to be cut out immediately after selecting a specific position, but can be cut out automatically after all the markings have been set.
  • the presence of microscopic sectional images of the respective preparation results in detailed information for the respective specialist staff, which selects the desired cutting positions.
  • the digital, microscopic images can also be divided into segments, which are combined with the image of the surface of the specimen before the overlay.
  • the markings or their coordinates are stored together with an identifier for the preparation in a database.
  • an identifier for the preparation can be, for example, a barcode or the like attached to the preparation or to the paraffin block surrounding the preparation. be educated.
  • sectional images can be selected for each preparation. These sectional images, which were recorded before the manipulation process, are also stored together with the identifier for the preparation in a corresponding database. To make it easier to set the markings at the desired cutting positions, the display scale of the sectional images, surface images and / or overlay images can be changed. Thus, the medical specialist can enlarge the areas of interest or get an overall view by reducing the display scale accordingly.
  • the objective method is also simplified by the fact that the sectional images, surface images and / or superimposition images can be shifted and their color changed. By changing the color, certain effects can be achieved which facilitate an assessment of the sectional images but also surface images or overlay images.
  • the degree of viewing of the surface images in relation to the sectional images can be changed according to a further method feature.
  • This so-called alpha value indicates how strongly the surface image of the preparation should shine through in comparison to the digital, microscopic sectional image of the preparation.
  • the transparency of the sectional images can be changed in order to enable an optimal representation of the overlay images.
  • patient information or the like associated with an identifier for the preparation can be provided. are displayed along with the overlay image.
  • This patient information or the like. is advantageously also stored in a database and is displayed, for example, on the screen to support the process of selecting the cutting positions.
  • the set markings can be marked accordingly, which can be done, for example, by means of a continuous numbering. This enables a clear identification of all markings.
  • Set markings can be selected and deleted in order to be able to undo a selection of cutting positions.
  • the diagnosis can be made easier later when evaluating the samples.
  • the set markings which correspond to the individual cutting positions of the samples, can be assigned to certain sample carriers for introducing the cut-out samples. If this option is not used, the existing sample carriers can, for example, be filled sequentially with the cut-out samples.
  • certain sample carriers are assigned to the set markings, certain holes in these sample carriers can also be assigned to the markings. In this way, an arrangement of the samples which may be advantageous for subsequent examinations of the microarrays or tissue arrays can be achieved.
  • the holes for the tissue samples are arranged in the sample holder in a pattern, which pattern by arranging the holes in the form of a Binary codes is formed. This ensures that the samples are clearly assigned. This prevents the glass carrier from delivering incorrectly assigned measurement results when the sample is cut by turning the carrier or rotating the carrier.
  • the samples can be arranged in many different patterns, which clearly define the direction of the microarray.
  • the position of the surface of the specimens is detected before the puncturing process and the detected position values are stored together with an identifier for the specimens.
  • the specimens usually have different heights, so that their surface must be detected in order to achieve exact cutting depths.
  • the detection of the surface can also be like, in which the samples are introduced. It is thereby achieved that the holes punched out in the sample carrier are always essentially exactly the same depth, so that the punched-out samples fit exactly into the holes in the sample carrier.
  • the detected position values of the sample carriers are also stored together with an identifier for the sample carriers so that they can be used in the subsequent automatic manipulation process.
  • the puncturing depth of the cutting and free-cutting operations can advantageously be selected and assigned to the markings.
  • different lancing depths can be defined for different cutting positions or different lancing depths can be selected for different preparations.
  • the automatic cutting process is started.
  • the preparations are positioned one after the other under the piercing needle and samples are cut out at the stored piercing positions, which are then introduced into the holes provided in the corresponding sample carriers.
  • the cutting process can be carried out automatically and quickly for a large number of samples.
  • the needle of impurities In order to clean the needle of impurities, it can be cleaned automatically after at least several piercing operations.
  • the punching needle can also be automatically guided to a cleaning station, where it can be cleaned with appropriate cleaning fluid and compressed air.
  • the second object of the invention is achieved by an above-mentioned device for manipulating samples, in particular tissue samples, in which a camera for recording images of the surface of the preparations, furthermore a device for superimposing the recorded images of the preparations with digital, stored in a memory.
  • a camera for recording images of the surface of the preparations furthermore a device for superimposing the recorded images of the preparations with digital, stored in a memory.
  • Microscopic images of sections of this preparation a display for displaying the superimposition images and a device for setting markings for determining the defined piercing positions are provided, which is connected to the control device for controlling the needles.
  • the device for superimposing the images is preferably formed by a computer. After the defined piercing positions have been defined, the corresponding control devices for controlling the piercing needle can be actuated accordingly, which results in automatic manipulation with the samples.
  • At least one needle is provided for pricking holes in sample carriers into which the cut-out samples are introduced.
  • a database is advantageously provided for storing the markings or their coordinates together with an identifier for the preparation and possibly patient information or the like.
  • This database can, for example, be integrated in a computer which already represents the device for superimposing the images, but can also be arranged externally and connected to the computer device or the like with corresponding data lines.
  • devices can be used to change the display scale, to change the orientation, to shift or be provided to change the color of the sectional images, surface images and / or superimposition images.
  • devices for changing the degree of transparency of the surface images with respect to the microscopic sectional images and devices for changing the transparency of the microscopic sectional images can be provided.
  • the above-mentioned devices are usually formed by a corresponding computer.
  • the device for setting markings for defining the defined cutting positions can be formed by a computer mouse.
  • a device for detecting the position of the surface of the sample carrier and / or preparations can be provided. By determining the exact position of the surface of the sample carriers and specimens, the holes in the sample carriers or the samples are always punched out exactly with the defined puncture depth.
  • a memory for the detected position values of the sample carriers or preparations together with an identifier of these sample carriers or preparations is advantageous.
  • This memory can be part of the database mentioned above.
  • At least one free-cutting needle and at least one piercing needle are advantageously mounted on a common swivel head, which can be actuated via a preferably pneumatic swivel drive.
  • the axis of the free-cutting needle and the piercing needle intersect at the pivot point of the swivel head.
  • a change between the free-cutting needle and the removal needle can thus be achieved by simply swiveling the swivel head.
  • only one drive device for the swivel head and not several drive devices need to be provided for each needle.
  • a drive device for displacing the swivel head relative to the sample carriers or specimens.
  • This can be arranged either in the swivel head or in the underlay or the documents for the sample carrier or specimens, so that the swivel head is displaced or the needles compared to the sample carriers or preparations can be achieved.
  • This drive device is also preferably pneumatic.
  • a computer program product which can be loaded directly into the internal memory of a digital computer and comprises software code sections also serves to solve the tasks according to the invention, the steps of the method described above being processed with the computer when the product is running on the computer.
  • the computer program product is preferably stored on a computer-readable medium.
  • Figure 1 is a schematic representation of a device for manipulation with samples, in particular tissue samples.
  • FIG. 2 shows a schematic block diagram of a device for manipulation with samples
  • Fig. 4 is a plan view of an embodiment of a sample holder equipped with several samples.
  • Preparations 5 can be biological or animal tissue parts, but also other samples, such as plants or parts of plants, cell suspensions or bacterial suspensions.
  • the swivel head 1 is displaceably arranged relative to a base 6 on which the sample carriers 4 and a base 6 'on which the specimens 5 are placed, so that the needles 2, 3 can be inserted into the specimen carriers 4 or specimens 5 ,
  • the documents 6, 6 ' are circular in the embodiment shown and arranged side by side, so that the paths of the swivel head 1 from the respective specimen 5 to the desired sample carrier 4 are minimal.
  • corresponding holders for receiving the sample carriers 4 or preparations 5 are provided on the bases 6, 6 '.
  • a selection of the desired preparations 5 or sample carriers 4 can be made.
  • a container 15 for receiving the ejected material of the sample carrier 4 or for cleaning the needles 2, 3 can be provided between the documents 6, 6 'for the sample carrier 4 or preparations 5.
  • the sample carriers 4 and specimens 5 are usually provided with a unique identifier, for example a barcode, which can be read in with a corresponding scanner 16.
  • the needles 2, 3 are arranged on the swivel head 1 in such a way that their axes intersect each other exactly at the swivel point of the swivel head 1. This ensures that the free-cutting needle 2 and the piercing needle 3 always come to lie exactly in the same position after a swiveling operation.
  • a preferably pneumatically actuated swivel drive 18 is used to swivel the swivel head 1.
  • This swivel drive 18 and the drive units 7, 8 are connected to a control device, which can be formed, for example, by a computer 13. Sections 9 are made from each preparation 5 and these are recorded with a microscope camera 10.
  • the recorded digital, microscopic sectional images are stored in a memory 11 connected to the computer 13.
  • the surface of the preparations 5 is recorded using a camera 14 and shown on a display 12.
  • the surface images of the preparations 5 recorded with the aid of the camera 14 are overlaid with selected sectional images of the same preparations 5 and displayed on the display 12.
  • markings can be placed on the overlay image which correspond to the desired piercing positions of the piercing needles 3.
  • a corresponding marking can be set on the monitor 12 using a computer mouse 17.
  • the superimposition images can be generated with the aid of the computer 13, for example with regard to the Stabes, the location, the color, etc., are changed.
  • the markings that have been set can subsequently be selected and deleted or moved again, and additional comments on the markings can be entered via the keyboard 19.
  • the comments are stored in a memory 11 together with the positions for the markings.
  • specific sample carriers 4 into which the samples are introduced can also be assigned to the selected cutting positions for the samples. Certain holes in the sample carriers 4 can even be selected for the respective sample. All this data is stored in the memory 11 or in another memory connected to the computer 13.
  • FIG. 2 shows a simplified block diagram of the device for manipulating samples comprising a computer 13 which is connected to a display 12. Furthermore, the computer 13 is connected to a microscope camera 10 for recording digital, microscopic sectional images 9 of the preparations 5 and to a camera 14 for recording the surface images of the preparations 5. After setting the appropriate markings for the cutting positions, an automatic cutting process is started and the swivel head 1, the rotary tables 6/6 'and the drive units 7, 8 and the swivel drive 18 of the swivel head 1 are controlled accordingly by the computer 13. In this way, a large number of samples can be processed automatically and arranged in appropriate sample carriers.
  • step 101 the identifier of the first sample carrier 4 is read in according to step 101. This is done, for example, with an appropriate scanner that reads the barcode arranged on the sample carrier 4.
  • query 102 it is checked whether a further sample carrier 4 is provided. The process 101 is repeated until all sample carriers 4 have been registered.
  • the height of the first sample carrier 4 is determined in accordance with block 103. As long as there are further sample carriers 4 according to query 104, step 103 is repeated and the height of all sample carriers 4 is determined.
  • block 105 the size of each sample carrier 4 is determined by specifying the number of samples provided in the sample carrier 4.
  • block 105 becomes for everyone Sample carrier 4 repeated.
  • the identifier of the first preparation 5 is read.
  • a surface image of the preparation 5 is produced and shown on a display.
  • a specific microscope image of this preparation 5 is selected from a memory and overlaid with the surface image recorded in block 108.
  • a marker is set, for example with the aid of a computer mouse, on which a sample is to be removed from the preparation 5.
  • Query 111 asks whether further markings are to be set, in which case block 110 is repeated correspondingly often.
  • the sample carrier desired for the samples of the preparation 5 is selected and assigned to the set markings.
  • FIG. 4 shows a plan view of a sample carrier 4 with a total of 487 positions for holes 20 for receiving 487 samples.
  • the holes 20 are arranged in a pattern which allows the samples to be clearly assigned even after cuts have been made.
  • the columns are binary coded with part of the holes 20. After the cuts, it is therefore not possible to mix up the samples by turning the glass carrier or twisting the glass carrier. Of course there are various other ways to achieve such clear assignments.
  • the present invention offers the information of microscope images and additionally allows the samples to be traced back to the cutting positions on the microscope image, and thus better documentation and quality control of the entire process.

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Multimedia (AREA)
  • Optics & Photonics (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L'invention concerne un procédé et un dispositif pour manipuler des échantillons, notamment des échantillons tissulaires, comprenant au moins une aiguille (3) qui prélève à des positions définies des échantillons dans des préparations (5), notamment dans des éléments tissulaires préparés, ainsi qu'un dispositif de commande (13) qui commande l'aiguille (3). L'invention vise à créer un procédé et un dispositif qui permettent une sélection simple des positions de prélèvement désirées sur les préparations (5), ainsi qu'une manipulation rapide, simple et automatique d'échantillons. A cet effet, le procédé et le dispositif selon l'invention utilisent une caméra (14) prenant des images de la surface des préparations (5), un dispositif superposant les images prises des préparations (5) à des images microscopiques numériques, stockées dans une mémoire, de coupes de ces préparations (5), un dispositif d'affichage (12) représentant les images de superposition, ainsi qu'un dispositif qui applique des marquages déterminant les positions de prélèvement définies et qui est relié au dispositif de commande (13).
EP03769021A 2002-10-31 2003-10-30 Procede et dispositif pour manipuler des echantillons Withdrawn EP1556476A2 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AT0164902A AT412239B (de) 2002-10-31 2002-10-31 Verfahren und vorrichtung zur manipulation mit proben
AT16492002 2002-10-31
PCT/AT2003/000324 WO2004039938A2 (fr) 2002-10-31 2003-10-30 Procede et dispositif pour manipuler des echantillons

Publications (1)

Publication Number Publication Date
EP1556476A2 true EP1556476A2 (fr) 2005-07-27

Family

ID=32046378

Family Applications (1)

Application Number Title Priority Date Filing Date
EP03769021A Withdrawn EP1556476A2 (fr) 2002-10-31 2003-10-30 Procede et dispositif pour manipuler des echantillons

Country Status (5)

Country Link
US (1) US20060147896A1 (fr)
EP (1) EP1556476A2 (fr)
AT (1) AT412239B (fr)
AU (1) AU2003277936A1 (fr)
WO (1) WO2004039938A2 (fr)

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Publication number Priority date Publication date Assignee Title
DE102007017807A1 (de) 2006-05-19 2008-02-14 Merz, Hartmut, Prof. Dr. med. Vorrichtung und Verfahren zur Herstellung von auf Objektträgern angeordneten Proben
US8072472B2 (en) 2006-06-26 2011-12-06 Agfa Healthcare Inc. System and method for scaling overlay images
AT505669B1 (de) * 2008-01-24 2009-03-15 Oridis Biomed Forschungs Und E Verfahren und vorrichtung zur manipulation mit proben
WO2013077297A1 (fr) * 2011-11-24 2013-05-30 オリンパス株式会社 Dispositif et procédé de tri de cellules
CN104583384B (zh) 2012-06-22 2020-03-17 莱卡生物系统努斯洛赫有限责任公司 活检组织样本运送装置和其使用方法
EP2864754B1 (fr) 2012-06-22 2020-08-26 Leica Biosystems Nussloch GmbH Récipient pour échantillon de tissu
US9389154B2 (en) 2013-03-15 2016-07-12 Leica Biosystems Nussloch Gmbh Tissue cassette with biasing element
CA2845830C (fr) 2013-03-15 2020-10-27 Leica Biosystems Nussloch Gmbh Cassette de tissu a element retractable
US9052256B2 (en) 2013-03-15 2015-06-09 Leica Biosystems Nussloch Gmbh Method for processing and embedding tissue

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US5843644A (en) * 1994-03-01 1998-12-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Isolation of cellular material under microscopic visualization using an adhesive/extraction reagent tipped probe
WO1999015875A1 (fr) * 1997-09-25 1999-04-01 Macquarie Research Ltd. Dispositif permettant de sortir un echantillon d'un ensemble d'echantillons et outil coupant pour ledit dispositif
DE19815400C2 (de) * 1998-04-06 2002-02-07 Max Planck Gesellschaft Vorrichtung zur Probenaufnahme aus polymeren Trägermaterialien
US6103518A (en) * 1999-03-05 2000-08-15 Beecher Instruments Instrument for constructing tissue arrays
JP2001100821A (ja) * 1999-09-30 2001-04-13 Japan Science & Technology Corp マニピュレータ制御方法および装置
US6383801B1 (en) * 2001-03-19 2002-05-07 Beecher Instruments Double z-drive tissue array instrument
DE10119461C2 (de) * 2001-04-17 2003-06-12 Ufz Leipzighalle Gmbh Verfahren und Vorrichtung zum Heraustrennen von Analysematerial aus einem Holzbohrkern

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Also Published As

Publication number Publication date
US20060147896A1 (en) 2006-07-06
ATA16492002A (de) 2004-04-15
AU2003277936A1 (en) 2004-05-25
WO2004039938A3 (fr) 2004-11-25
AT412239B (de) 2004-11-25
WO2004039938A2 (fr) 2004-05-13

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