EP1455778A2 - Verfahren zur behandlung von peripheren nerven- und gefässerkrankungen - Google Patents

Verfahren zur behandlung von peripheren nerven- und gefässerkrankungen

Info

Publication number
EP1455778A2
EP1455778A2 EP02789474A EP02789474A EP1455778A2 EP 1455778 A2 EP1455778 A2 EP 1455778A2 EP 02789474 A EP02789474 A EP 02789474A EP 02789474 A EP02789474 A EP 02789474A EP 1455778 A2 EP1455778 A2 EP 1455778A2
Authority
EP
European Patent Office
Prior art keywords
composition
skin
flavonoid
quercetin
pharmaceutically acceptable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02789474A
Other languages
English (en)
French (fr)
Other versions
EP1455778A4 (de
Inventor
Richard A. Rosenbloom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Prophase Labs Inc
Original Assignee
Quigley Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2001/049297 external-priority patent/WO2002049575A2/en
Application filed by Quigley Corp filed Critical Quigley Corp
Publication of EP1455778A2 publication Critical patent/EP1455778A2/de
Publication of EP1455778A4 publication Critical patent/EP1455778A4/de
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/07—Retinol compounds, e.g. vitamin A
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
    • A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline 
    • A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
    • A61K31/355—Tocopherols, e.g. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
    • A61K31/365—Lactones
    • A61K31/375—Ascorbic acid, i.e. vitamin C; Salts thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
    • A61K31/7048—Compounds having saccharide radicals and heterocyclic rings having oxygen as a ring hetero atom, e.g. leucoglucosan, hesperidin, erythromycin, nystatin, digitoxin or digoxin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/82—Theaceae (Tea family), e.g. camellia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A61K8/355—Quinones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
    • A61K8/42—Amides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
    • A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
    • A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4986—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with sulfur as the only hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/60—Sugars; Derivatives thereof
    • A61K8/602—Glycosides, e.g. rutin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/671—Vitamin A; Derivatives thereof, e.g. ester of vitamin A acid, ester of retinol, retinol, retinal
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/676—Ascorbic acid, i.e. vitamin C
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/67—Vitamins
    • A61K8/678—Tocopherol, i.e. vitamin E
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9771—Ginkgophyta, e.g. Ginkgoaceae [Ginkgo family]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/14—Drugs for dermatological disorders for baldness or alopecia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/16—Emollients or protectives, e.g. against radiation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/08—Antiepileptics; Anticonvulsants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth

Definitions

  • the present invention relates to compositions and methods for the treatment of peripheral neural and vascular ailments.
  • a flavonoid is administered to a patient suffering from a peripheral neural or vascular ailment.
  • peripheral neuropathies and “small fiber neuropathies” are used interchangeably herein to refer to a set of conditions characterized by functional changes or pathological changes, or both, in the small, unmyelinated nerve fibers of the peripheral nervous system.
  • diabetic neuropathy is not included among the peripheral small fiber neuropathies.
  • Peripheral or small fiber neuropathies may be caused by any of about a hundred identified factors that can produce nerve damage. The cause may be metabolic, for example hypertriglyceridemia or pellagra.
  • Toxic exposures may also cause small fiber neuropathies, for example those resulting from alcoholism, excessive doses of vitamin B 6 , exposure to toxic metals such as thallium, or exposure to certain chemotherapeutic agents, such as vinca alkaloids.
  • Certain congenital conditions including amyloidosis, an- ⁇ -lipoproteinemia (Tangier's), and - galactosidase (Fabry's), are known to cause small fiber neuropathies.
  • Small fiber neuropathies may result from infections such as leprosy, or diseases such as AIDS, herpes simplex, herpes zoster (shingles), cytomegalovirus, hepatitis B and C, Lyme disease, autoimmune diseases, Fabry disease, diphtheria, vasculitis, and porphyria.
  • infections such as leprosy, or diseases such as AIDS, herpes simplex, herpes zoster (shingles), cytomegalovirus, hepatitis B and C, Lyme disease, autoimmune diseases, Fabry disease, diphtheria, vasculitis, and porphyria.
  • AIDS herpes simplex
  • herpes zoster cytomegalovirus
  • hepatitis B and C Lyme disease
  • autoimmune diseases Fabry disease
  • diphtheria diphtheria
  • vasculitis and porphyria
  • peripheral neuropathies Patients afflicted with peripheral neuropathies have pain in their extremities. The pain may at first be perceived as a tingling sensation in the fingers or toes. Decreased sensitivity to heat or cold is also a common early symptom. Frequently, however, a physical examination will show that the patient's reflexes, strength, sensory levels, and electrophysiology are normal. This has historically complicated the diagnosis of peripheral neuropathies, or led to underdiagnosis, especially in the early stages of the neuropathies. Recent technology, however, including skin biopsies and measurement of the density of different nerve fiber types in the epidermis, has improved the likelihood of detecting peripheral neuropathies.
  • Small fiber or peripheral neuropathies tend to progress by spreading upward, and patients may develop intense pain and/or a burning sensation that can be so severe as to be debilitating.
  • Other symptoms of these neuropathies include cold hands or feet, cramps, muscle weakness and/or atrophy, eventual loss of perception of pressure, pain and/or temperature, neuropathic ulcers, lack of sweating, dry eyes, dry mouth, impotence, and restless leg syndrome.
  • treatment of the underlying cause may also reverse or alleviate small fiber neuropathies.
  • treatment consists of reducing the symptoms of the neuropathies, typically by administering medications known to decrease pain from neuropathy and related conditions. These medications include tricyclic antidepressants, anticonvulsants, opioid medications, and local anesthetics applied to the painful area.
  • An afflicted patient may also undergo physical and occupational therapy to improve mobility and function.
  • peripheral neuropathies do not vary due to their underlying causes.
  • a neuropathy caused by exposure to chemotherapeutic agents may present symptoms almost identical to those of a neuriopathy caused by Lyme disease.
  • diabetic neuropathy is a fairly common long-term complication of diabetes mellitus that shares many of the symptoms of peripheral neuropathies.
  • the cause of diabetic neuropathy is believed to be a chronic systemic excess of the glucose metabolite sorbitol.
  • treating the underlying cause of diabetic neuropathy that is, improving glycemic control, will often prevent the symptoms from worsening.
  • Diabetic neuropathy is also known to be reversible, if good glycemic control is instituted while the condition is in its early stages.
  • Peripheral Neuropathies can also impair circulation in the affected area. Impaired circulation can adversely affect the appearance of the skin. Adverse effects on the appearance of the skin caused by radiation injury can include, for example, redness, discoloration, dryness of the skin.
  • "Peripheral vascular diseases” are diseases of the blood vessels outside the heart that lead to restriction or blockage of the blood vessels. Atherosclerosis, when it affects the extremities rather than the coronary arteries, is an example of a peripheral vascular disease. Peripheral vascular diseases may also be long-term complications of other diseases, such as Raynaud's disease, Raynaud's phenomenon, hypertension, or Buerger's disease (thromboangitis obliterans).
  • peripheral vascular disease An early symptom of peripheral vascular disease includes pain upon exercising that is relieved by rest. These diseases are progressive, however, and patients may also experience numbness, muscle weakness or pain, loss of hair on the affected extremities, cyanosis, weak or absent pulse in the affected extremities, gait abnormalities, pain when resting, skin ulcers, and, eventually gangrene. Impaired circulation caused by peripheral vascular disease can also adversely affect the appearance of the skin. Adverse effects on the appearance of the skin caused by radiation injury can include, for example, redness, discoloration, dryness of the skin. In general, an agent that promotes or induces angiogenesis, or one that at least partially clears blocked or restricted vessels, or one that will facilitate peripheral circulation by other means, i.e. by decreasing cellular adhesion, will be effective to treat peripheral vascular diseases.
  • rnicrocirculation is also a long-term complication of diabetes.
  • a therapy that is effective for peripheral vascular diseases will also be effective to counter decreased rnicrocirculation caused by diabetes.
  • a clinically effective treatment of peripheral vascular ailments there remains a need for a clinically effective treatment of peripheral vascular ailments.
  • the present invention provides a method for the treatment of peripheral neural and vascular ailments by administering a composition including a therapeutically effective amount of a flavonoid having antioxidant properties, and, optionally, an acceptable carrier.
  • the invention in another embodiment, relates to a composition for treating peripheral neural and vascular ailments.
  • the composition comprises a therapeutically effective amount of a mixture of a flavonoid having antioxidant properties, a therapeutically effective amount of a non-flavonoid antioxidant compound, and, optionally, an acceptable carrier.
  • compositions and methods of the invention provide significant, effective relief of symptoms of peripheral neural and vascular ailments, as well as partial recovery of lost rnicrocirculation or neurological function in some cases. Surprisingly, the efficacy of the invention is not dependent on the underlying causes of the peripheral neural and vascular ailments.
  • the composition used in the method of the invention when administered in a therapeutically effective amount to treat peripheral neural and vascular ailments, does not cause severe side effects.
  • the topical compositions and methods of the invention also treat adverse effects on the appearance of the skin caused by peripheral neuropathies and/or peripheral vascular disease. These cosmetic benefits are obtained in patients having such disorders. Adverse effects on the appearance of the skin include, for example, redness, discoloration, dryness.
  • the invention treats or cosmetically improves the appearance of persons having peripheral or neural vascular ailments by, for example, reducing or preventing redness of skin, reducing or preventing discoloration of skin, beautifying skin, improving appearance of skin, promoting attractiveness of skin, cleansing skin, removing dead or damaged skin or skin cells from skin and moisturizing skin.
  • compositions and methods of the invention also provide nutritional and/or dietary benefits. These nutritional or dietary cosmetic benefits are obtained in patients having peripheral neuropathies and/or peripheral vascular disease. Such nutritional or dietary effects are also intended to be included within the meaning of "treating ailments.”
  • the invention offers dietary or nutritional benefits in supporting and/or maintaining neural, vascular and muscular health, maintains sensory integrity, i.e. sensations of hot and cold, and supports the maintenance of skin health.
  • derivatives refers to structurally similar compounds that exhibit a common activity (e.g., antioxidant) and contain at least one significant, common structural element with the compound from which it is derived, which common structural element provides the common activity.
  • therapeutically effective amount refers to a nontoxic amount of a compound which is sufficient to provide the desired therapy to counteract small fiber neuropathies or peripheral vascular diseases.
  • a therapeutic amount may, for example, reduce pain, reverse sensory fiber loss or demyelination, promote angiogenesis, increase rnicrocirculation, or increase sensory perception.
  • the exact amount required may vary, depending on the species, age, and general condition of the patient, the nature of the complications, the particular combination of compounds, the mode of administration, and the like.
  • the term “therapeutically” is intended to encompass beneficial cosmetic effects and effects of improved nutrition as well as medical effects.
  • compositions used in the method of the present invention include at least one flavonoid.
  • Flavonoids are small organic compounds having a phenyl benzopyrone structure. They are found in the leaves, fruits, seeds, stems, or flowers of all vascular plants. Citrus fruits are a prominent source of flavonoids, over 4000 of which have been identified as deriving from plant sources. On average, the daily Western diet contains about one gram of mixed flavonoids.
  • flavonoids include, without limitation, flavonones, flavonols, anthocyanidins, proanthocyanidins, procyanidolic oligomers, biflavans, polyphenols, rutinosides, hydroxyethylrutinosides, and leucoanthocyanins.
  • Suitable flavonoids for use in the present invention include those that do not induce significant, adverse side effects when administered to a mammal in a therapeutically effective amount, and that do not react with any of the other ingredients of the composition used in the present invention to cause a substantial loss of activity of one or more compounds of the composition.
  • Preferred flavonoids are obtained from natural sources. However, derivatives of such compounds may also be suitable for use in the present invention. Preferred flavonoids may be administered to humans without significant, adverse side effects when used in therapeutically effective amounts.
  • the selection of the flavonoid(s) included in the composition may be determined by factors such as toxicity, bioavailability, solubility or dispersability, and the like.
  • flavonoids suitable for use in the present invention include, without limitation, (-)-epigallocatechin; (-)-epigallocatechin-gallate; 1,2,3,6-tetra-o- gallyol- ⁇ -d-glucose; 2'-o-acetylacetoside; 3,3',4-tri-o-methyl-ellagic acid; 6,3',4'- trihydroxy-5,7,8-trimethoxyflavone; 6-hydroxy-luteolin; 6-hydroxykaempferol-3,6- dimethyl ether; 7-o-acetyl-8-epi-loganic acid; acacetin; acetoside; acetyl trisulfate quercetin; amentoflavone; apigenin; apiin; astragalin; avicularin; axillarin; baicalein; brazilin; brevifolin carboxylic acid; caryophyllene; catechin; chrysin;
  • Preferred flavonoids are those that also have strong antioxidant properties.
  • preferred flavonoids include, without limitation, (-)-epigallocatechin-3- gallate, catechin, rutin, quercetin, quercitrin, myricetin, kaempferol, myrecetrin luteolin, morin, fisetin, silymarin, apigenin, hesperitin, hesperidin, citrin, gossypetin, chrysin, oligomeric proanthocyanidins, biacalein, curcumin, gallic acid, epicatechin, dihydroquercetin, ginkgo flavone glycosides, ginkgo heterosides, silibin, silydianin silychristine, galangin, monoHER, diHER, triHER, tetraHER, naringenin, naringin, taxifolin, diosmin, phloretin, phloridzin, cyani
  • flavonoids include, without limitation, quercetin, quercitrin, myricetin, rutin, kaempferol and myrecetrin. These compounds exhibit good antioxidant properties in combination with relatively low toxicity.
  • flavonoids and flavonoid derivatives may provide additional beneficial effects in the composition of the present invention.
  • quercetin acts as a chelator for transition metals.
  • Flavonoids are also believed to possess anti- inflammatory activity and to assist in the stabilization of cell membranes, both activities that promote the treatment of small fiber neuropathies. Quercetin is also believed to have anticlastogenic properties.
  • some flavonoids and flavonoid derivatives act as radical scavengers, reducing the concentration of hydroxyl radicals, for example, and thereby further enhancing the antioxidant effect of the composition used in the present invention.
  • Suitable non-flavonoid antioxidants for use in the present invention include those that exhibit antioxidant activity without causing any severe adverse side affects when administered in a therapeutically effective amount, and that do not react with any of the other ingredients of the composition used in the present invention to cause a substantial loss of activity of one or more compounds.
  • Preferred antioxidants include those that occur naturally in the human body and materials obtained from plants or animals, or derivatives of such compounds.
  • Preferred non-flavonoid antioxidants include, without limitation, ascorbyl palmitate, ascorbic acid (vitamin C), vitamin A, vitamin E and its pharmaceutically acceptable esters (including but not limited to the acetate), ⁇ -lipoic acid, especially DL- ⁇ -lipoic acid, coenzyme Q10, glutathione (GSH), galangin, gingkolides, tocotrienols, carotenoids, cyanidin, curcuminoids, and derivatives thereof which exhibit antioxidant activity. More preferably, mixtures of two or more antioxidants are employed in the composition used in the present invention. Derivatives of one or more of these compounds that exhibit antioxidant activity when administered in the compositions of the present invention may also be employed.
  • the antioxidants may also be used in the form of their pharmaceutically acceptable salts. The salts may be preferred in some cases, for example to increase solubility or dispersability, or to reduce adverse side effects.
  • the antioxidant used in the composition of the present invention may comprise one or more antioxidant enzymes.
  • the antioxidant enzymes useful in the present invention are those capable of scavenging radicals, of promoting radical scavengers or preventing radical formation.
  • One or more of these antioxidant enzymes may act synergistically with one or more of the other antioxidants in the composition to scavenge free radicals more effectively and thereby aid in the prevention of cell damage in the skin.
  • the antioxidant enzyme used in the present invention is capable of abso ⁇ tion through the skin.
  • Preferred antioxidant enzymes for use in the present invention include superoxide dismutase, catalase, glutathione peroxidase, methionine reductase, and the like.
  • both quercetin and an antioxidant are included in the composition of the present invention.
  • This combination of quercetin and an antioxidant results in an enhanced anti-oxidative effect.
  • the antioxidant may be a flavonoid or a non-flavonoid.
  • compositions used in the present invention may also be included in the composition of the present invention to provide additional benefits, such as absorbability when applied topically, free radical scavenging, transition metal chelation, nitric oxide stabilization, analgesia, and anti-inflammatory activity. Some of these properties may have a beneficial effect on the pain of other related disorders such as fibromyalgia.
  • Additional materials that may optionally be included in the compositions used in the present invention include inositol and other B-complex vitamins.
  • Some preferred compositions used in the invention also contain vitamin D , a vitamin D 3 analog, a compound that may be converted or metabolized into vitamin D 3 in the human body, or a metabolite of vitamin D 3 .
  • Vitamin D 3 also known as cholecalciferol
  • Vitamin D 3 may be further converted into another vitamin D intermediate, 25-hydroxycholecalciferol, in the liver by rnitochondrial hydroxylase, in the presence of NADPH, and molecular oxygen.
  • vitamin D 3 When a more active form of vitamin D 3 is required, 25-hydroxycholecalciferol is transported to the kidney where a new hydrolase enzyme is synthesized. This enzyme introduces another hydroxyl group at position 1, and the bioactive form of vitamin D 3 , calcitriol, is produced.
  • An exemplary vitamin D 3 analog is 1(S), 3(R)-dihydroxy-20(R)-(l-ethoxy-5- ethyl-5-hydroxy-2-heptyn-l-yl)-9, 10-seco-pregna-5(Z), 7(E), 10 (19)-triene.
  • An exemplary vitamin D 3 metabolite is 1, 25-dihydroxyvitamin D 3 .
  • Pharmaceutically acceptable salts of vitamin D 3 and its derivatives and metabolites may be employed in the methods of the present invention.
  • Vitamin D 3 is particularly preferred for use in the present invention.
  • a dispersant may be necessary to facilitate the formulation of the vitamin D 3 or related compound. Suitable dispersants are well known to persons skilled in the art.
  • Corn oil is one dispersant that is well suited for vitamin D 3 and related compounds. Also advantageously, corn oil is a natural product. The corn oil is used in an amount sufficient to disperse the vitamin D 3 or related compound.
  • compositions used in the present invention may provide one or more of the following localized or systemic beneficial effects to a patient when administered in therapeutically effective amounts: relief of pain, burning, tingling, electrical sensations and/or hyperalgesia; increased rnicrocirculation; nitric oxide stabilization; promotion of healing of skin ulcers and lesions; protein kinase C inhibition; decreased oxidative stress; anti-inflammatory activity; blockage of the formation of leukotrienes; stabilization of cell membranes; and promotion of the synthesis of nerve growth factor.
  • compositions in accordance with the invention can provide additional effects of improving the appearance of the skin. Skin appearance may be adversely affected by peripheral neuropathies, including diabetic neuropathy, and/or peripheral vascular disease, or by other causes unrelated to the peripheral neuropathies and/or peripheral vascular disease being treated.
  • peripheral neuropathies including diabetic neuropathy, and/or peripheral vascular disease, or by other causes unrelated to the peripheral neuropathies and/or peripheral vascular disease being treated.
  • One or more of the following beneficial properties may be realized when compositions of the invention are topically applied in an effective amount: reducing or preventing redness of skin, reducing or preventing discoloration of skin, beautifying skin, improving appearance of skin, promoting attractiveness of skin, cleansing skin, removing dead or damaged skin or skin cells from skin and moisturizing skin.
  • an effective treatment might cause the degeneration of peripheral nerves to slow or to stop.
  • an effective treatment might induce healing or regeneration of the damaged nerves.
  • An effective treatment might also cause the generation of new nerves to replace the damaged nerves. It is therefore expected that effective treatments for small fiber neuropathies will be applicable to other diseases or conditions affecting peripheral nerves.
  • a method of regenerating nerves is beneficial to treat any patient suffering from nerve damage, for example, a skin graft patient or a victim of a nerve-severing trauma.
  • many flavonoids are potent aldose reductase inhibitors. It has been shown that the oral administration of aldose reductase inhibitors increases the diameter of peripheral nerve bundles. Thus, it is expected that the methods of the present invention extend to the generation and regeneration of nerves.
  • the underlying cause of diabetic neuropathy is specifically known to be distinct from the other causes of small fiber neuropathies, the symptoms and pathologies are shared. It is therefore expected that an effective treatment for small fiber neuropathies will arrest, reverse, or alleviate certain symptoms of diabetic neuropathy. It has also been found that for treatment of diabetic neuropathy, the optional component, vitamin D 3 or a derivative or metabolite thereof, may be excluded from the composition and the composition will still provide a beneficial effect, provided the composition includes other suitable components. Flavonoids promote rnicrocirculation and therefore can also be used to treat peripheral vascular diseases. For example, the flavonoid quercetin supports vascular functioning in general, and therefore is an effective treatment for peripheral vascular diseases.
  • rnicrocirculation is believed to be caused at least in part by oxidative stress resulting from an excess of free radicals.
  • Quercetin an example of a flavonoid with antioxidant properties, is therefore an effective treatment for this condition.
  • the chelating properties of flavonoids such as quercetin contribute to its effectiveness.
  • the overabundance of sorbitol in the bloodstream of diabetic patients attracts metal ions, which are sequestered by chelation.
  • compositions used in the present invention are preferably formulated with an acceptable carrier.
  • the non-carrier ingredients may be combined with the carrier materials to produce a particular dosage form, or be customized for a particular treatment regimen.
  • the amount of each ingredient may vary depending on such factors as the particular mode of administration, the activity of the particular compounds employed, the age, bodyweight, general health, sex, and diet of the patient, time of administration, rate of excretion, the combination of compounds, or the severity of the illness, among other potential factors.
  • a standard reference text on pharmaceutical formulations Remington's Pharmaceutical Sciences, 18 th Ed., Mack Publishing Co. 1990, is inco ⁇ orated herein by reference in its entirety.
  • composition refers to stable mixtures, compositions, and formulations, respectively. Preferred mixtures, compositions, and formulations are stable over a period of at least about three months.
  • the composition may be administered via several routes, including, without limitation, topically, orally, via an implanted reservoir, or by inhalation.
  • the composition is administered orally.
  • An oral composition for use in the invention may be administered one to six times daily, or as needed to relieve pain and other symptoms of the small fiber neuropathies.
  • the composition when administered orally, is administered two to four times daily, as needed for pain.
  • a sufficient amount should be administered to provide one or more of the beneficial effects of the compositions described above.
  • the method initially treats acute symptoms but may be continued indefinitely to relieve pain, prevent symptoms from returning and possibly restore some nerve and/or skin function.
  • the oral compositions and methods of the invention also function as dietary or nutritional supplements.
  • oral compositions and methods can maintain and/or support neural health, maintain and/or support vascular health and circulation, maintain and/or support muscular health, maintain sensory integrit, i.e. sensations of hot and cold, and support the health of the skin.
  • compositions used in the present invention may be orally administered in any acceptable dosage form including, but not limited to, capsules, tablets, lozenges, troches, hard candies, powders, sprays, elixirs, syrups, and suspensions or solutions.
  • Suitable acceptable carriers for tablets include lactose and corn starch, for example.
  • Lubricating agents may also be added to the tablets, including, for example, magnesium stearate, sodium lauryl sulfate and talc. Tablets may also contain excipients such as sodium citrate, calcium carbonate and calcium phosphate.
  • Disintegrants such as starch, alginic acid and complex silicates, may also be employed. Tablets may also include binding agents such as polyvinylpyrrolidone, gelatin and gum acacia.
  • composition used in the invention may be administered in capsule form, with or without diluents.
  • diluents for capsules include, without limitation, lactose and dried cornstarch.
  • solid compositions similar to those of the tablets described above may be administered in soft and hard gelatin capsules.
  • compositions used in the invention may be administered orally as encapsulated or unencapsulated suspensions, and they may comprise emulsifying and/or suspending agents such as are well known to those of skill in the art.
  • Ancillary ingredients such as sweeteners, flavorants, coloring agents, dyes, and diluents such as water, ethanol, propylene glycol, glycerin and various combinations thereof may also be included in the oral formulations.
  • compositions used in the present invention may also be administered by nasal aerosol or by inhalation.
  • Appropriate formulations may be prepared using well- known techniques.
  • suitable carriers include, for example, saline and/or other conventional solubilizing or dispersing agents, optionally formulated with one or more preservatives, abso ⁇ tion promoters to enhance bioavailability, and/or fluorocarbons.
  • the composition is applied topically to an area of the skin in the vicinity of tissue that suffers from small fiber neuropathy in order to relieve pain and other symptoms of the small fiber neuropathy.
  • Such areas typically include the patients' extremities, such as the fingers, toes, hands and feet, where neuropathy is often most pervasive.
  • a suitable amount of the topical composition of the invention is applied one to six times daily, as needed. More preferably, the topical composition is applied two to four times daily, as needed. Also preferably, a sufficient amount of the topical composition is applied to cover the afflicted area with a thin layer of the composition and the composition is rubbed into the skin until little or no residue remains on the skin.
  • the treatment is almost immediately effective to alleviate acute symptoms, and may be continued, for a predetermined period or indefinitely, to relieve pain, prevent the return of symptoms of small fiber neuropathies, and possibly restore some nerve and/or skin function.
  • a topical formulation of the composition used in the invention preferably includes an acceptable topical carrier.
  • Many acceptable topical carriers are known to those of skill in the art.
  • the compounds in the composition may be dissolved, dispersed and/or suspended in the topical carrier.
  • hydrophilic ointment bases are known to persons skilled in the art.
  • Exemplary hydrophilic ointment bases suitable for use in the present invention are non-U.S.P. hydrophilic ointment bases such as those made by Fougera, Inc., a division of Altana, Inc. of Melville, NY.
  • Sufficient hydrophilic ointment base is employed to act as a carrier for the compounds of the composition.
  • the hydrophilic ointment base will make up more than 80% of the total composition and more preferably 80-90% of the composition is the hydrophilic ointment base.
  • the hydrophilic ointment base functions as a carrier and preferably enhances penetration of the compounds into the skin.
  • One preferred topical carrier comprises hydroxymethyl cellulose.
  • Another preferred acceptable carrier includes a solution of an acrylic copolymer in a non- aqueous solvent system.
  • the non-aqueous solvent system preferably contains a polyethylene glycol such as, for example, methoxy polyethylene glycol 550 (MPEG).
  • MPEG methoxy polyethylene glycol 550
  • One preferred MPEG is Sentry Carbowax MPEG 550 (Dow Co ⁇ ., Midland, MI), which is suitable for use in foods, pharmaceuticals, and cosmetics.
  • the acrylic copolymer is preferably present in a concentration range of 3-6% by weight of solution. Also preferably, the acrylic copolymer has a molecular weight of more than 20,000.
  • the acrylic copolymer has a molecular weight of more than 100,000, to substantially prevent abso ⁇ tion of the acrylic copolymer by the human body through the skin.
  • the acceptable topical carrier independently provides benefits to the patient.
  • the topical carrier may comprise panthenol or a panthenol derivative.
  • the panthenol derivatives useful in the present invention include at least D-panthenol, DL-panthenol, and mixtures thereof. Panthenol provides skin moisturizing properties, acts as a quick, deep penetrating component of the carrier, helps deliver the compounds through the skin to the area to be treated, and may impart a healing effect to damaged tissue.
  • panthenol or panthenol derivative preferably ranges from 0.25 to 10 weight percent, more preferably from 0.5 to 5 weight percent, and, still more preferably, from 1 to 2 weight percent, based on the total weight of the topical composition.
  • the topical carrier of the present invention may employ other penetrants in addition to panthenol or as an alternative to panthenol.
  • Exemplary penetrants include ethanol, oleic acid, sodium lauryl sulfate, isopropyl myristate, glycerol monooleate, caprylic/capric triglyceride, Crodamol GTC/C, glyceryl tricaprylate/caprate, Miglyol 810, Miglyol 812, MCT oil, Neobee M5, Nesatol, oleum neutrale, oleum vegetable whatsoever, thin vegetable oil, light mineral oil, stearyl alcohol and lanolin mixed with suitable vegetable oils or with soft paraffin. These penetrants may have an emollient effect and facilitate the abso ⁇ tion of ingredients of the topical composition of the present invention into the skin.
  • the topical carrier of the present invention contains at least a hydrophilic ointment base, panthenol or a panthenol derivative, and one or more dispersants, if needed to disperse an insoluble or partially insoluble compounds in the carrier.
  • the topical carrier of the present invention may also include additional ingredients well known to persons skilled in the art, such as other carrier materials, other moisturizers, humectants, emollients, radiation blocking compounds, particularly UV-blockers, as well as other suitable materials that do not have a significant adverse effect on the activity of the topical composition in the amount used.
  • a preferred additional ingredient for inclusion in the carrier is sodium acid phosphate, a moisturizer.
  • the topical composition of the present invention is preferably made by cold compounding, when one or more of the compounds employed in the topical composition are known to be sensitive to heat. Thus, in some cases, the stability or activity of the composition may be detrimentally affected as a result of other formulation methods.
  • a sufficient amount of the topical carrier is used, to provide a substantially homogeneous cream or ointment. It may be necessary to dissolve, disperse or suspend one or more of the ingredients prior to formulation in order to ensure substantially homogeneous distribution of one or more of the ingredients in the composition.
  • dosages may vary with the manner of formulating the compounds.
  • the components of the composition which include the flavonoid and the optional antioxidant, will make up from 0.5-90% by weight of the total composition to provide the desired daily dosage.
  • the body weight dosages herein, when not normalized, are based on a patient having a body weight of 70 kg.
  • the appropriate unit dosage may be determined by dividing the daily dosage by the number of unit doses per day.
  • the at least one flavonoid of the present invention is administered in a safe and effective amount. Every pound of a preferred topical composition of the present invention preferably includes about 1 to about 150 grams of one or more flavonoids, about 0.1 to about 50 grams of non-flavonoid antioxidants, and other suitable ingredients such as topical carriers. Preferably, the flavonoid is used in an amount of about 2 to about 100 grams per pound of the composition. More preferably, the flavonoid is employed in an amount of about to about 10-50 grams per pound of the composition, and, still more preferably, about 15 to about 40 grams per pound of the composition.
  • the ratio of the amount of that compound to the amount of the flavonoid employed in the compositions of the present invention is from about 200 IU per gram of antioxidant to about 3 million IU per gram of flavonoid. More preferably, the composition contains about 1800 IU to about 1 million IU of nerve growth factor synthesis promoter per gram of flavonoid, and, still more preferably, about 5000 IU to about 200,000 IU of nerve growth factor synthesis promoter per gram of flavonoid.
  • the composition includes both vitamins A and D 3 , they are preferably formulated together in a corn oil dispersion.
  • each cubic centimeter (cc) or milliliter (mL) of the corn oil dispersion contains about 500,000 to about 2,000,000 IU of vitamin A and about 50,000 to about 200,000 IU of vitamin D 3 .
  • every milliliter of the corn oil contains about 800,000 to about 1,200,000 IU of vitamin A and about 80,000 IU to about 120,000 IU of vitamin D 3 .
  • the composition used in the invention contains about 1,000,000 IU and about 100,000 IU of vitamins A and D 3 , respectively.
  • an amount of about 6 to about 14.3 IU per kg of body weight of the patient for each administration More preferably, an amount of about 8 to about 14.3 IU per kg body weight of the patient, ' and, still more preferably, an amount of about 10 to about 13 IU is employed per kg of body weight of the patient, is administered.
  • the flavonoid is preferably used in an amount that provides substantially the same level of activity as a daily dose of about 13 to about 22 mg/kg bodyweight of quercetin. More preferably, the flavonoid is administered in an amount that provides substantially the same level of activity as a daily dose of about 17.2 to about 21.4 mg/ kg bodyweight of quercetin, and, still more preferably, an amount that provides substantially the same level of activity as a daily dose of about 18 to about 21 mg/kg bodyweight of quercetin.
  • about 11 to about 29 mg/kg bodyweight/day of ascorbyl palmitate may be administered. More preferably, about 14.3 to about 28.6 mg/kg bodyweight/day is administered.
  • the daily dosage is preferably about 4 to about 12 IU per kg bodyweight. More preferably, the daily dosage is about 5.7 to about 11.4 IU per kg bodyweight. Still more preferably, the daily dosage of mixed tocopherols is about 6 to about 10 IU per kg bodyweight.
  • an amount is administered that provides an equivalent effect as the above-described amounts of mixed tocopherols.
  • the daily dosage is preferably about 170 to about 360 IU per kg bodyweight per day. More preferably, the dosage is about 214.3 to about 357.1 IU per kg bodyweight per day. Still more preferably, the dosage is about 220 to about 340 IU per kg bodyweight per day.
  • Every pound of a preferred topical composition of the present invention preferably includes about 2 to about 50 grams of one or more flavonoids, about 1 to about 50 grams of non-flavonoid antioxidants, as well as other suitable ingredients such as topical carriers.
  • the witch hazel extract may be used in an amount of about 2.5 - 40 cc, more preferably of about 5 - 30 cc, and most preferably of about 10 - 20 cc per pound of topical base.
  • the glycerine humectant may be used in an amount of about 2-20 cc, more preferably of about 3.5 - 15 cc, and most preferably of about 5 - 10 cc per pound of topical base.
  • the apricot kernel oil may be used in an amount of about 0.5 - 5 cc, more preferably of about 0.5 - 4 cc, and most preferably of about 1 - 3 cc per pound of topical base.
  • the AJIDEWNL-50 NaPCA (50% aqueous solution) may be used in an amount of about 15 - 45 cc, more preferably of about 20 - 40 cc, and most preferably of about 25 - 35 cc per pound of topical base.
  • a more preferred topical composition of the invention can be made using the following ingredients: about 25 to about 35 cc of a 50% aqueous solution of AJIDEWNL-50 NaPCA (50% aqueous solution) moisturizing agent, about 5 to about 10 cc of D- or DL-panthenol, and about 10 to about 50 grams of quercetin powder.
  • hydrophilic ointment base As is well known in the art, larger amounts of one or more components, e.g. an antioxidant, can be employed while reducing the amount of another component of the same type or having a similar type of activity.
  • one or more components e.g. an antioxidant
  • about lOg/kg bodyweight of quercetin is used.
  • about 5g/kg bodyweight to about 25g/kg bodyweight, more preferably about 5g/kg bodyweight, of rutin are added to the composition.
  • about lOg to about 50g/kg bodyweight, more preferably about lOg/kg bodyweight, of glutathione are added to the composition.
  • compositions are substantially free of cinnamic acid derivatives of the formula:
  • groups X, Y and R independently of one another, can be chosen from the group consisting of H and branched or unbranched alkyl having 1-18 carbon atoms, acids thereof, and physiologically tolerated salts thereof.
  • EXAMPLE 1 A topical composition including a mixture of an hydrophilic ointment base, sodium acid phosphate moisturizing agent, and DL-panthenol, formulated together as the acceptable carrier, and further including quercetin was prepared by cold compounding.
  • the formulation of the composition is given in Table 1.
  • the formulation may optionally be supplemented with coenzyme Q10 (500mg) and may optionally contain another antioxidant,.
  • the composition was prepared by first placing the hydrophilic ointment base in a stainless steel bowl and mixing briskly until the ointment became creamy. The sodium acid phosphate, panthenol, quercetin, and other anti-oxidant, if any, were next added in that order. After each ingredient was added, mixing was continued until no traces of dry ingredients were visible and a substantially homogeneous mixture was obtained. The final color was a consistent yellow and the cream had the consistency of cake frosting. The mixture was stored in a sterile container. All containers and tools that contact the composition during mixing must also be sterilized with, for example, zephiran chloride, a bleach solution, or betadine.
  • This composition can be topically administered, under the supervision of a physician, to patients diagnosed with small fiber neuropathies.
  • the topical composition may be applied, for example, twice daily in the morning and afternoon, or up to six times daily, as needed for pain relief, over a period of a few days. Treated patients are predicted to experience positive results that will last up to a day or two after treatment is discontinued.
  • Examples 2 through 7 Other combinations of compounds suitable for use in the methods of the invention are set forth in Examples 2 through 7.
  • the compounds may be combined with about lib of hydrophilic ointment base for topical administration.
  • EXAMPLE 2 A topical composition was formulated using the ingredients listed in Table 2 below.
  • the composition of Table 2 was topically administered three times per day over a period of 4 weeks to 24 patients suffering from diabetic neuropathy in at least one foot as a result of having Type 1 or Type 2 diabetes mellitus in a placebo- controlled, double blind proof of concept study conducted in France. 12 patients received a placebo consisting of the composition of Table 2 except that the ascorbyl palmitate and quercetin dihydrate were left out. Eligible patients were screened using the Michigan Neuropathy Screening Instrument (MNSI), Feldman, E ., et al, "A Practical Two-Step Quantitative Clinical and Elecfrophysiological Assessment for the Diagnosis and Staging of Diabetic Neuropathy," Diabetes Care, 19 , pp. 1281-1289.
  • MNSI Michigan Neuropathy Screening Instrument
  • Feldman E ., et al, "A Practical Two-Step Quantitative Clinical and Elecfrophysiological Assessment for the Diagnosis and Staging of Diabetic Neuropathy," Diabetes Care, 19 , pp. 1281-1289.
  • the results of the test were positive.
  • the formulation produced a significant decrease of diabetic peripheral neuropathy pain and discomfort, and an improvement in the appearance and texture of the skin, including reduced dryness.
  • a similar formulation without ascorbyl palmitate was also effective on diabetic skin and aging skin, as well as providing relief from the discomfort of diabetic peripheral neuropathy and small fiber peripheral neuropathy.
  • Vitamin E acetate lcc
  • Vitamin E acetate lcc
  • Vitamin E acetate lcc

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Birds (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Dermatology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Neurology (AREA)
  • Diabetes (AREA)
  • Toxicology (AREA)
  • Pain & Pain Management (AREA)
  • Biomedical Technology (AREA)
  • Cardiology (AREA)
  • Neurosurgery (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Emergency Medicine (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Molecular Biology (AREA)
  • Rheumatology (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Obesity (AREA)
  • Hematology (AREA)
EP02789474A 2001-12-19 2002-11-06 Verfahren zur behandlung von peripheren nerven- und gefässerkrankungen Withdrawn EP1455778A4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
WOPCT/US01/49297 2001-12-19
PCT/US2001/049297 WO2002049575A2 (en) 2000-12-21 2001-12-19 Method and composition for the treatment of diabetic neuropathy
PCT/US2002/035654 WO2003053336A2 (en) 2001-12-19 2002-11-06 Methods for the treatment of peripheral neural and vascular ailments

Publications (2)

Publication Number Publication Date
EP1455778A2 true EP1455778A2 (de) 2004-09-15
EP1455778A4 EP1455778A4 (de) 2007-09-12

Family

ID=21743140

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02789474A Withdrawn EP1455778A4 (de) 2001-12-19 2002-11-06 Verfahren zur behandlung von peripheren nerven- und gefässerkrankungen

Country Status (8)

Country Link
EP (1) EP1455778A4 (de)
JP (1) JP2005518381A (de)
CA (1) CA2470603A1 (de)
IL (1) IL162505A0 (de)
MX (1) MXPA04006039A (de)
NZ (1) NZ533439A (de)
WO (1) WO2003053336A2 (de)
ZA (1) ZA200404614B (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2543404A1 (en) * 2003-10-24 2005-05-06 Meiji Seika Kaisha, Ltd. Novel inhibitor of the formation of advanced glycation end product and aldose reductase inhibitor
EP1681297A4 (de) * 2003-10-30 2009-06-17 Meiji Seika Kaisha Inhibitor der tyrosinaseaktivität und mittel zum verbessern der durchblutung des gesichts
JP2007510725A (ja) 2003-11-05 2007-04-26 オステオスクリーン，インコーポレイテッド イチョウフラバノイド類による発毛の刺激
JP2006083105A (ja) * 2004-09-16 2006-03-30 Ito En Ltd 血液流動性改善剤および飲食物
US20070111953A1 (en) * 2005-06-28 2007-05-17 Kgk Synergize, Inc. Compositions to improve the bioavailability of polymethoxyflavones and tocotrienols for treatment of cardiovascular disease
DE102005048778A1 (de) * 2005-10-10 2007-04-12 Beiersdorf Ag Kosmetische Formulierungen zur Verbesserung des Aussehens der Haut
DE102005048777A1 (de) * 2005-10-10 2007-04-12 Beiersdorf Ag Kosmetische Formulierungen zur Verbesserung der Mikrozirkulation der Haut
JP5132889B2 (ja) * 2006-02-27 2013-01-30 株式会社ポッカコーポレーション 神経成長因子増強剤
AU2007235487A1 (en) * 2006-04-05 2007-10-18 Vertex Pharmaceuticals Incorporated Deazapurines useful as inhibitors of janus kinases
FR2904557B1 (fr) * 2006-08-01 2010-04-30 Pf Medicament Nouvel extrait d'eucalyptus, son procede de preparation et ses utilisations therapeutiques
GB0618168D0 (en) * 2006-09-15 2006-10-25 Babraham Inst Compounds
US20090060879A1 (en) * 2007-09-04 2009-03-05 The Procter & Gamble Company Oral Compositions, Products and Methods Of Use
US20100075926A1 (en) * 2008-07-23 2010-03-25 Li-Huei Tsai Activation of histone deacetylase 1 (hdac1) protects against dna damage and increases neuronal survival
JP5415216B2 (ja) * 2009-10-02 2014-02-12 日本メナード化粧品株式会社 皮膚外用剤
CN102266320B (zh) * 2010-07-20 2015-06-17 浙江养生堂天然药物研究所有限公司 橙皮素的新用途
KR101021835B1 (ko) 2010-09-06 2011-03-17 충남대학교산학협력단 크리소스플레놀 시를 함유하는 심장 질환의 예방 및 치료용 조성물
CN102151281B (zh) * 2011-01-24 2014-03-26 中国药科大学 防治糖尿病的黄酮类化合物及其药物用途
RS58438B1 (sr) 2011-07-22 2019-04-30 Massachusetts Inst Technology Aktivatori klase i histonskih deacetilaza (hdacs) i njihova primena
NZ596015A (en) * 2011-10-26 2014-04-30 Anzamed Internat Ltd Method and composition for the treatment of pain and/or inflammation
CN102526248B (zh) * 2012-03-22 2013-07-17 赵海潞 一种治疗糖尿病伤口溃烂并可持续降血糖的中药组合物
US20180193306A1 (en) 2012-03-23 2018-07-12 Cardero Therapeutics, Inc. Compounds and compositions for the treatment of muscular disorders and bone disorders
EP2827856A4 (de) * 2012-03-23 2016-03-09 Cardero Therapeutics Inc Verbindungen und zusammensetzungen zur behandlung von muskelkrankheiten
ITRM20120335A1 (it) * 2012-07-13 2014-01-14 Aboca Spa Societa Agricola Nuove composizioni per il trattamento del dolore neuropatico.
WO2014095289A2 (en) * 2012-12-20 2014-06-26 Unilever Plc Method of treating hair ageing
US9844531B2 (en) 2013-03-15 2017-12-19 Abbott Laboratories Methods of maintaining and improving muscle function
CN104547872A (zh) * 2015-01-30 2015-04-29 山东省立医院 一种治疗癫痫的中药组合物
CN107126437A (zh) * 2016-02-29 2017-09-05 上海中医药大学 一种槐角苷的药物用途
KR101828379B1 (ko) 2016-03-30 2018-02-12 우석대학교 산학협력단 해당화 꽃 추출물 또는 분획물을 유효성분으로 함유하는 기억력 개선, 인지능력 개선, 치매 예방, 지연, 개선 또는 치료용 조성물
KR101715294B1 (ko) * 2016-06-09 2017-03-10 연세대학교 산학협력단 디오스민 또는 이의 염을 유효성분으로 함유하는 피부보습 개선, 피부각질 제거, 피부탄력 증진, 홍반 억제, 피부주름 개선 또는 피부광노화 개선 효과를 갖는 조성물
WO2018078412A1 (en) * 2016-10-31 2018-05-03 Posi Visionary Solutions Llp Topical microemulsion of diosmetin and uses thereof
CN108164489B (zh) * 2018-03-20 2019-11-26 武汉轻工大学 一种高良姜素的制备方法
BR102018005599A2 (pt) * 2018-03-21 2019-10-08 Pedro Barboza Da Rocha Composição cicatrizante, hidratante, umectante e emoliente para administração tópica em lesões de pele intertrigo, lesões de pele por incontinência urinária e fecal e outras lesões afins.
WO2020051644A1 (en) * 2018-09-13 2020-03-19 Norbio No. 2 Pty Ltd Treatment of peripheral nervous tissue inflammation
CN112014480B (zh) * 2019-05-28 2023-03-28 黄河科技学院 一种uplc-ms/ms检测降脂宁颗粒中有效成分含量的方法
WO2022157381A1 (en) * 2021-01-25 2022-07-28 Universiteit Hasselt Phloretin for use in the treatment of neurodegenerative and demyelinating diseases
CN115317497A (zh) * 2022-01-13 2022-11-11 宁夏医科大学 甘草苷在制备胸主动脉血管舒张药物中的用途
CN114392256B (zh) * 2022-02-22 2023-05-09 中山大学附属第八医院（深圳福田） 矢车菊素在防治血管钙化中的应用
WO2024112947A1 (en) * 2022-11-23 2024-05-30 Brightseed, Inc. Compositions and methods for modulating sleep and uses thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001076580A1 (en) * 2000-04-11 2001-10-18 Takara Bio Inc. Remedies
WO2002049575A2 (en) * 2000-12-21 2002-06-27 The Quigley Corporation Method and composition for the treatment of diabetic neuropathy

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH03232851A (ja) * 1989-12-26 1991-10-16 Nippon Flour Mills Co Ltd アルドース還元酵素阻害剤
JPH09235284A (ja) * 1995-02-23 1997-09-09 Nissan Chem Ind Ltd インドール系チアゾリジン類
IT1291113B1 (it) * 1997-03-20 1998-12-29 Sigma Tau Ind Farmaceuti Composizione nutritiva terapeutica per soggetti affetti da diabete mellito
IT1302307B1 (it) * 1998-09-01 2000-09-05 Sigma Tau Healthscience Spa Composizione ad attivita' antiossidante ed atta a migliorarel'utilizzazione metabolica del glucosio, comprendente acetil
JP4497566B2 (ja) * 1998-09-02 2010-07-07 株式会社 日本薬用食品研究所 アルドース還元酵素阻害剤並びに新規サポニン及び新規サポゲノール

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001076580A1 (en) * 2000-04-11 2001-10-18 Takara Bio Inc. Remedies
EP1283037A1 (de) * 2000-04-11 2003-02-12 Takara Bio Inc. Arzneien
WO2002049575A2 (en) * 2000-12-21 2002-06-27 The Quigley Corporation Method and composition for the treatment of diabetic neuropathy

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
SCHOEMAKER J H: "PHARMACOLOGICAL TREATMENT OF DIABETIC PERIPHERAL NEUROPATHY: CHALLENGES AND POSSIBILITIES" BRITISH JOURNAL OF CLINICAL PRACTICE, vol. 48, no. 2, 1994, pages 91-96, XP000878610 ISSN: 0007-0947 *
See also references of WO03053336A2 *
VARMA ET AL: "Flavonoids as Inhibitors of lens Aldose reductase." SCIENCE, vol. 188, 1995, - 1995 pages 1215-1216, XP002431692 *

Also Published As

Publication number Publication date
ZA200404614B (en) 2005-08-29
EP1455778A4 (de) 2007-09-12
WO2003053336A3 (en) 2003-11-27
IL162505A0 (en) 2005-11-20
AU2002352501A1 (en) 2003-07-09
CA2470603A1 (en) 2003-07-03
JP2005518381A (ja) 2005-06-23
WO2003053336A2 (en) 2003-07-03
NZ533439A (en) 2006-06-30
MXPA04006039A (es) 2004-09-27

Similar Documents

Publication Publication Date Title
US7410659B2 (en) Methods for the treatment of peripheral neural and vascular ailments
EP1455778A2 (de) Verfahren zur behandlung von peripheren nerven- und gefässerkrankungen
US6555573B2 (en) Method and composition for the topical treatment of diabetic neuropathy
US20030105031A1 (en) Methods for the treatment of skin disorders
AU2002309615B2 (en) Nutritional supplements and methods for prevention, reduction and treatment of radiation injury
US6753325B2 (en) Composition and method for prevention, reduction and treatment of radiation dermatitis
US7399783B2 (en) Methods for the treatment of scar tissue
US20030118536A1 (en) Topical compositions and methods for treatment of adverse effects of ionizing radiation
US7435725B2 (en) Oral compositions and methods for prevention, reduction and treatment of radiation injury
AU2002309615A1 (en) Nutritional supplements and methods for prevention, reduction and treatment of radiation injury
CA2431079C (en) Method and composition for the treatment of diabetic neuropathy
AU2002352501B2 (en) Methods for the treatment of peripheral neural and vascular ailments
US20020165207A1 (en) Compositions and methods for the treatment of diabetic neuropathy
TW200526236A (en) Methods for the treatment of peripheral neural and vascular ailments

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20040609

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LI LU MC NL PT SE SK TR

AX Request for extension of the european patent

Extension state: AL LT LV MK RO SI

A4 Supplementary search report drawn up and despatched

Effective date: 20070813

17Q First examination report despatched

Effective date: 20090317

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20101108