Classifications

• • C—CHEMISTRY; METALLURGY
  • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
  • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
  • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
  • C12N15/09—Recombinant DNA-technology
  • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
  • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle

Definitions

• the present invention provides a storage system for chemical or biological stock solutions in form of encapsulated chemical or biological stock mixtures, which can be readily used in the preparation of any chemical or biological solution of choice, e.g. buffers, bacterial media, gel solutions and other commonly used stock solutions, that are typically employed in analytical and diagnostic techniques in the life sciences.
• any chemical or biological solution of choice e.g. buffers, bacterial media, gel solutions and other commonly used stock solutions, that are typically employed in analytical and diagnostic techniques in the life sciences.
• any chemical or biological solution e.g. buffers, bacterial media, gel solutions and the like
• analytical and diagnostic techniques are a daily requirement in every basic research and diagnostic laboratory in the life sciences, such as in the areas of molecular biology and cell biology.
• These solutions are used in techniques that may range from simple procedures, e.g. the preparation of cell culture media, to highly sophisticated techniques, e.g. PCR, and their successful performance depends on an accurate and reliable preparation of the chemical or biological solutions employed.
• the present invention provides a storage system for chemical or biological solutions in form of encapsulated chemical or biological stock mixtures, which allows rapid preparation of virtually any type of buffer solution to be employed in virtually any type of analytical and diagnostic technique. Moreover, such encapsulated chemical or biological stock mixtures may be easily prepared, are user-friendly and cost-effective and thus may improve the efficiency of any analytical and diagnostic technique it is used for.
• chemical or biological solutions contemplated include any chemical or biological solution typically used in the life sciences, such as buffers or buffer mixtures, media, e.g. bacterial media, gel solutions, any other commonly used stock solutions, as well as combinations thereof.
• buffers include commonly used buffers well known in the art (see for example Sambrook, Fritsch, Maniatis, Molecular Cloning, A Laboratory manual, 2 nd Edition, Vol 3, Appendix B), such as various buffers used in molecular biology techniques, and include for example
• Tris-buffers e.g. Tris-glycine-, Tris-NaCl-, Tris-EDTA(TE)-, Tris-borate-EDTA (TBE)-, TAE-buffer and Tris-buffered saline (TBS), HEPES-, MOPS-, PIPES-, MES-, PBS-, PBP- buffers and the like.
• any of these buffers may be present in combination with a medium, a gel solution such as agarose or any other commonly used stock solution.
• any of the above buffer solutions may be present in form of a buffer mixture comprising in addition further components, such as for example pH modifiers, indicators (dyes), surfactants, stabilizers, sugars, proteins, and the like, depending on their intended use in a particular technique.
• Such components which may be present in buffers at concentrations well-known in the art, e.g. 1 x to 10 x, include, e.g.
• the above buffers may be employed in various, typically used concentrations, e.g. 1 x to 50 x, preferably 1 x to 10 x, to yield working buffer solutions or stock solutions of desired concentration.
• bacterial media examples include commonly used bacterial media well known in the art at well-known concentrations, e.g. 1 x to 10 x, (see for example Sambrook, Fritsch, Maniatis, Molecular Cloning, A Laboratory manual, 2 nd Edition, Vol 3, Appendix A), such as liquid media, e.g. LB Medium, NZCYM Medium, NZYM Medium, NZM Medium, Terrific Broth, SOB Medium, SOC Medium, 2xYT Medium, M9 Minimal Medium and Media containing agar or agarose.
• concentrations e.g. 1 x to 10 x, (see for example Sambrook, Fritsch, Maniatis, Molecular Cloning, A Laboratory manual, 2 nd Edition, Vol 3, Appendix A)
• liquid media e.g. LB Medium, NZCYM Medium, NZYM Medium, NZM Medium, Terrific Broth, SOB Medium, SOC Medium, 2xYT Medium, M9 Minimal Medium and Media containing agar
• Typical examples of agar include, e.g. Anaerobiose-agar, Hefeex Exercise-Cystein-Blut (HCB-)agar, Mueller-Hinton-agar, Salmonella-Shigella-(SS)agar, Thayer-Martin-agar, Anaerobic Bloodagar according to CDC, Hefeex Install-Cystein-Blut modified with Hämin (HCBH-)agar, and the like.
• gel solutions include for example solutions of agarose, e.g. standard agarose, agarose high, agarose medium, agarose low melt; agar solutions, e.g. DNA agar; acrylamide/bisacrylamide, and the like, which can be encapsulated alone or in combination with suitable buffer components.
• Said gel solutions may be used at concentrations well-known in the art, e.g. at concentrations to yield a final concentration of a gel solution of choice ranging from e.g. 0.5 % to 5 %.
• the capsules so prepared are ready to be dissolved in exact amounts of bidest water, e.g. 50 ml, 75 ml, 100 ml depending on the size of the gel.
• the encapsulated chemical or biological stock mixtures of the present invention may be prepared by mixing and filling appropriate amounts of the components of the chemical or biological solution of choice into a capsule, preferably a gelatine capsule, e.g. a hard gelatine capsule, of for example 2g, 3g or 5g size.
• a gelatine capsule e.g. a hard gelatine capsule, of for example 2g, 3g or 5g size.
• the preparation of a desired buffer solution from these capsules may comprise either (i) dissolving an appropriate number of capsules in their entirety in an appropriate amount of liquid, e.g. bidest water, under continuous stirring and optionally heating for a certain amount of time, e.g. 1 minute, in a microwave or (ii) opening an appropriate number of capsules and mixing their content with an appropriate amount of liquid, e.g. bidest water, under continuous stirring and optionally heating for a certain amount of time, e.g. 1 minute, in a microwave, to yield a chemical or biological solution of desired composition and concentration.
• an appropriate amount of liquid e.g. bidest water
• Example 1 Gelatine capsules comprising 0.5x TBE
• Example 2 Gelatine capsules comprising 0.5x TBE and 1 % agarose

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• 2002
  • 2002-06-18 EP EP02405500A patent/EP1375659A1/fr not_active Withdrawn

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