EP1322763A2

EP1322763A2 - Listeria inocua , genome and applications

Info

Publication number: EP1322763A2
Application number: EP01982519A
Authority: EP
Inventors: Fréderik KUNST; Philippe Glaser
Original assignee: Centre National de la Recherche Scientifique CNRS; Institut Pasteur de Lille
Current assignee: Centre National de la Recherche Scientifique CNRS; Institut Pasteur de Lille
Priority date: 2000-10-04
Filing date: 2001-10-04
Publication date: 2003-07-02
Also published as: FR2814754B1; KR20030064404A; WO2002028891A2; AU2002214081A1; US20040018514A1; FR2814754A1; WO2002028891A3; CA2424952A1; JP2004515227A

Abstract

The invention concerns a nucleotide sequence derived from Listeria inocua corresponding to a sequence selected among SEQ ID NO: 1 to SEQ ID NO: 11 and the comparative analysis of said genome with that of Listeria monocytogenes.

Description

LISTERIA INNOCUA, GENOME AND APPLICATIONS

The subject of the invention is a method making it possible to demonstrate the specific nucleotide sequences of the genome of a strain of bacteria of the genus Listeria, in particular of a strain of L. innocua or L. monocytogenes. The subject of the present invention is also the genomic sequence and nucleotide sequences coding for Listeria innocua polypeptides, such as cell envelope polypeptides, secreted or specific, or involved in metabolism and in the replication process, as well as vectors including said sequences and cells or animals transformed by these vectors. The invention also relates to the comparison of these nucleotide sequences with those coding for the polypeptides of Listeria monocytogenes, strain EGDe or L. monocytogenes 4b, as well as the nucleotide sequences specific for these strains of Listeria. The invention also relates to methods for detecting these nucleic acids or polypeptides and to kits for diagnosing contamination by bacteria of the genus Listeria and kits for typing contaminating strains. The invention also relates to a method of selecting compounds capable of modulating the bacterial infection caused by other Listeria and a method of biosynthesis or biodegradation of molecules of interest using said nucleotide sequences or said polypeptides. The invention finally comprises pharmaceutical compositions, in particular vaccine compositions, for the prevention and / or treatment of bacterial infections, in particular by Listeria, in particular monocytogenes, and compositions containing antibodies directed against specific polypeptides of L. innocua or of L. monocytogenes, strain EGDe or L. monocytogenes 4b. In Listeria infections, Listeria monocytogenes is the most common and the most dangerous. Listeria monocytogenes is a facultative intracellular pathogen. It is the etiological agent of listeriosis, a food-related infection posing increasing public health problems, with a significant economic impact for the food industry. Listeriosis is the most lethal food-borne infection (approximately 30% mortality). Listeria monocytogenes has the unusual property of being able to cross three barriers: the intestinal barrier, the blood-brain barrier and the placental barrier. The clinical manifestations of listeriosis include meningitis, meningoencephalitis, abortion and septicemia. This infection is opportunistic and mainly affects pregnant women, babies, the elderly and people who are immunosuppressed, especially people with AIDS. This disease also affects healthy individuals and is responsible for a significant number of epidemics due to contaminated food products. Listeria monocytogenes is also of veterinary importance with a main risk for sheep (sheep) and cattle. Listeria monocytogenes is particularly resistant to stress or extreme conditions and it is important to look for its presence carefully not only for food safety problems but also for environmental safety issues.

Following the discovery of a contamination, the typing of the isolated strain (s) is necessary to identify the origin of the contamination. Furthermore, when the same installation is contaminated by two successive events, it is important to show with certainty whether these are two independent contaminations or if the same strain is responsible for these two events. The most efficient method currently used, the gel migration profile in pulsed fields (PFGE) after digestion of chromosomal DNA is a very cumbersome method which cannot be implemented systematically. An alternative method, less efficient but automated, ribotyping, presents a high cost, per analysis, which limits its use.

It should also be noted that the risk of listeriosis is very variable depending on the contaminating Listeria strain. In the extreme, some strains could be considered dangerous and others harmless (like Listeria innocua). Thus, while Listeria contaminations are very frequent, the number of cases described is low. In this perspective, the availability of a tool to identify the risk associated with contamination (depending on the genomic type of the strain and the number of bacteria per gram of food) would allow manufacturers to react based on this risk .

The complete genome sequence of Listeria monocytogenes was established for the EGDe strain deposited at the CNCM under the n ° 1-2440 on April 1, 2000 and described in the French patent application N ° 00 04629 filed on April 1, 2000. The genome of this bacterium is circular and contains around 3000 kilobases. Its GC content is around 38%. Studies of virulence factors have made it possible to identify a locus of 15 kb which can be considered to be an island of pathogenicity insofar as it contains most of the genes whose function in virulence has been clearly identified. The subject of the present invention is therefore a method making it possible to demonstrate nucleotide sequences specific for the genome of a strain of bacteria of the genus Listeria, in particular specific for a strain of L. innocua or L. monocytogenes, such as the strain L monocytogenes EGDe or L. monocytogenes 4b. Such a method according to the invention peπnet in particular the identification of specific sequences of:

- L. innocua compared to L. monocytogenes, in particular compared to L. monocytogenes EGDe and / or L. monocytogenes 4b;

- L. monocytogenes, in particular L. monocytogenes EGDe or L. monocytogenes 4b, compared to L. innocua;

- L. monocytogenes EGDe compared to L. innocua and / or L. monocytogenes 4b; and

- L. monocytogenes 4b compared to L. innocua and / or L. monocytogenes EGDe. Said method according to the invention is preferably characterized in that it comprises at least the following steps: a) alignment of the nucleotide sequences of L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b, and those of L. innocua according to the invention; and b) processing the data obtained by this alignment to isolate said specific sequences.

In a preferred embodiment, the method according to the invention is characterized in that the nucleotide sequences of L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b are chosen from the genomic nucleotide sequences: - such as described in French patent application N ° 00 04629 filed on

April 1, 2000 or in the international patent application PCT / FR 01/01 1 18 filed on April 1, 2001 for L. monocytogenes EGDe, in particular the sequence SEQ ID No. 1 of the complete genome of L. monocytogenes EGDe; and

- the sequences SEQ ID Nos. 1068 to 2041 or Nos. 2872 to 3891 for L. monocytogenes 4b.

In an also preferred embodiment, the method according to the invention is characterized in that the nucleotide sequences specific for L. inocua or L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b, hybridize in high stringency conditions with the sequences respectively nucleotides, or their complementary sequence, of L. inocua or L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b.

The present invention relates to the nucleotide and polypeptide sequences of Listeria innocua and the comparison of the corresponding sequences with those of Listeria monocytogenes strain EGDe and / or 4b.

The invention relates in particular to:

- the nucleic sequences SEQ ID Nos. 12 to 689 (see Table V) and SEQ ID Nos. 2059 to 2601 (cf. Table VI), in particular SEQ ID Nos. 2059 to 2601, specific for Listeria innocua compared to Listeria monocytogenes strain EGDe; - the nucleic sequences SEQ ID Nos. 690 to 1067 (see Table V) and SEQ ID

Our. 2602 to 2871 (cf. Table VII), in particular SEQ ID Nos. 2602 to 2871, specific for Listeria monocytogenes strain EGDe compared to Listeria innocua;

- the nucleic sequences SEQ ID Nos. 3892 to 4025 (cf. Table IX) specific for Listeria monocytogenes 4b compared to Listeria innocua and Listeria monocytogenes strain EGDe, their fragments of sufficient length to retain their aforesaid specificity, their complementary sequence, primers or specific probes, the peptides encoded by these nucleic acid sequences or antibodies directed against these peptides, as well as in particular their uses, for the identification of a strain of Listeria, or for the distinction between a pathogenic or non-pathogenic strain of Listeria in a biological sample, in particular using diagnostic methods or kit as below presented or known to those skilled in the art.

Those skilled in the art will be able, from these specific sequences according to the invention, to draw the primers or probes, to produce the specific peptides or the antibodies directed against these peptides necessary for the implementation of these diagnostic methods or the 'development of diagnostic kit as below presented or standard.

It is therefore an object of the present invention to disclose the complete sequence of the genome of Listeria innocua, in particular CLIP 1 1262 contained in the genomic bank prepared from the genome of this strain and deposited at the CNCM on October 2, 2000 under the number 1-2565 as well as all the non-coding regulatory genes and sequences contained in said genome. The CLIP 1 1262 strain was isolated from a dairy product. This strain is kept at the National Reference Center of Listeria at the INSTITUT PASTEUR (WHO collaborating center).

The comparison of the complete sequences of the genomes of L. monocytogenes strain EGDe and Listeria innocua, strain CLIP 1 1262, shows that approximately 86% of these genomes are very highly conserved (80 to 95% DNA identity). On the other hand, the remaining 14% are specific to each strain. In practice, a chip representing all the genes of each species would give a positive signal for the DNA of the two strains for 86% of the probes and for 14% a signal only with the DNA of one of the two strains.

These results are in agreement with data from the literature on the diversity of Listeria strains. Furthermore, recent laboratory data on the sequencing of an epidemic strain of L. monocytogenes (serotype 4b (CLIP 80459)) confirms this diversity but above all shows that the strains of serotype-4b are probably as close to L. innocua as of the L. monocytogenes serotype-1 / 2a strain whose genome has been sequenced. The CLIP 80459 strain is an epidemic strain. It is kept at the National Reference Center of Listeria of the INSTITUT PASTEUR

(WHO collaborating center). It should also be emphasized that the Innocua strain is not pathogenic and therefore that the specific genes of L. monocytogenes are potentially involved in pathogenicity. Furthermore, the analysis of the genome of the EGDe strain has made it possible to identify the main skill genes, that is to say the genes promoting horizontal gene transfers. Certain strains of Listeria must therefore have the capacity to be transformed. Horizontal transfers between strains must therefore be frequent and explain the great diversity observed between isolates.

The Listeria monocytogenes serotype 4b strain is also identified in the present application by Listeria monocytogenes 4b and interchangeably.

All of these observations indicate that the genes identified as variables between L. monocytogenes strain EGDe and L. innocua must be representative of the genomic diversity of Listeria.

The invention also relates to new tools for typing Listeria strains. These tools could be of the DNA "chip" type or of another type. The new features of these typing tools will be as follows:

* Speed and simplicity of use; * High power of discrimination between strains;

* Possibility of providing information on the genomic content of the strain analyzed and possibly making it possible to predict the risk associated with contamination by Listeria. The present invention therefore relates to a nucleotide sequence of Listeria innocua characterized in that it corresponds to a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058, in particular from SEQ ID No. 2057 and SEQ ID No. 2058.

The present invention also relates to a nucleotide sequence derived from Listeria innocua, characterized in that it is chosen from: a) a nucleotide sequence comprising at least 75%, 80%, 85%, 90%, 95% or 98% of identity with a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058, in particular from SEQ ID No. 2057 and SEQ ID No. 2058; b) a nucleotide sequence hybridizing under conditions of high stringency with a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058; c) a nucleotide sequence complementary to a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058, or complementary to a nucleotide sequence as defined in a) , or b), or a nucleotide sequence of the RNA corresponding to one of the sequences a) or b); d) a nucleotide sequence of a fragment representative of a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058, or of a fragment representative of a nucleotide sequence as defined in a), b) or c); e) a nucleotide sequence comprising a sequence as defined in a), b), c) or d); and f) a nucleotide sequence as defined in a), b), c), d) or e) modified. More particularly, the present invention also relates to the nucleotide sequences characterized in that they come from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058 and in what they code for a polypeptide, chosen from the sequences SEQ ID No. 12 to SEQ ID No. 689, SEQ ID No. 2053 to SEQ ID No. 2056 and SEQ ID No. 2059 to SEQ ID No. 2601, in particular from SEQ ID No. 2059 to SEQ ID No. 2601. The present invention also relates more generally to the nucleotide sequences originating from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID

No. 2058 and coding for a polypeptide of L. innocua, as they can be isolated from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058, in particular to from SEQ ID No. 2057 and SEQ ID No. 2058.

In addition, the nucleotide sequences, characterized in that they comprise a nucleotide sequence chosen from: a) a nucleotide sequence coding for a polypeptide, chosen from the sequences SEQ ID No. 12 to SEQ ID No. 689, SEQ ID No. 2053 to SEQ ID No. 2056 and SEQ ID No. 2059 to SEQ ID No. 2601, in particular from SEQ ID No. 2059 to SEQ ID No. 2601; b) a nucleotide sequence comprising at least 75%, 80%, 85%, 90%, 95% or 98% of identity with a nucleotide sequence coding for a polypeptide, chosen from the sequences SEQ ID No. 12 to SEQ ID No 689, SEQ ID No. 2053 to SEQ ID No. 2056 and SEQ ID No. 2059 to SEQ ID No. 2601, in particular from SEQ ID No. 2059 to SEQ ID No. 2601; c) a nucleotide sequence hybridizing under high stringency conditions with a nucleotide sequence coding for a polypeptide, chosen from the sequences SEQ ID No. 12 to SEQ ID No. 689, SEQ ID No. 2053 to SEQ ID No. 2056 and SEQ ID No. 2059 to SEQ ID No. 2601, in particular from SEQ ID No. 2059 to SEQ ID No. 2601; d) a complementary nucleotide or RNA sequence corresponding to a sequence as defined in a), b) or c); e) a nucleotide sequence of a fragment representative of a sequence as defined in a), b), c) or d); and f) a sequence as defined in a), b), c), d) or e) modified, are also objects of the invention.

The present invention also relates to a nucleotide sequence of Listeria monocytogenes serotype 4b of sequence SEQ ID No. 1068 to SEQ ID No. 2041 and SEQ ID No. 2872 to SEQ ID No. 3891, in particular SEQ ID No. 2872 to SEQ ID No. 3891.

The present invention also relates to a nucleotide sequence of Listeria monocytogenes serotype 4b characterized in that it is chosen from: a) a nucleotide sequence comprising at least 75%, 80%, 85%, 90%, 95% or 98% identity with SEQ ID No. 1068 to SEQ ID No. 2041, SEQ ID No. 2872 to SEQ ID No 3891, in particular with SEQ ID No. 2872 to SEQ ID No. 3891; b) a nucleotide sequence hybridizing under conditions of high stringency with SEQ ID No. 1068 to SEQ ID No. 2041, SEQ ID No. 2872 to SEQ ID

No. 3891, in particular with SEQ ID No. 2872 to SEQ ID No. 3891; c) a nucleotide sequence complementary to SEQ ID No. 1068 to SEQ ID No. 2041, SEQ ID No. 2872 to SEQ ID No. 3891, in particular from SEQ ID No. 2872 to SEQ ID No. 3891 or complementary to a sequence nucleotide as defined in a) or b), or an RNA nucleotide sequence corresponding to one of the sequences a) or b); d) a nucleotide sequence of a fragment representative of SEQ ID No. 1068 to SEQ ID No. 2041, SEQ ID No. 2872 to SEQ ID No. 3891, in particular from SEQ ID No. 2872 to SEQ ID No. 3891 or d 'a fragment representative of a nucleotide sequence as defined in a), b) or c); e) a nucleotide sequence comprising a sequence as defined in a), b), c) or d); and f) a nucleotide sequence as defined in a), b), c), d) or e) modified. More particularly, the present invention also relates to the nucleotide sequences characterized in that they come from SEQ ID No. 1068 to SEQ ID No. 2041, SEQ ID No. 2872 to SEQ ID No. 3891, in particular from SEQ ID No. 2872 to SEQ ID No. 3891 and in that they code for a polypeptide, chosen from the sequences SEQ ID No. 690 to SEQ ID No. 1067, SEQ ID No. 2049 to SEQ ID No. 2052 and SEQ ID No. 2602 to SEQ ID No. 2871, in particular from SEQ ID No. 2602 to SEQ ID No. 2871.

The present invention also relates more generally to the nucleotide sequences originating from SEQ ID No. 1068 to 2041, SEQ ID No. 2872 to SEQ ID No. 3891, in particular from SEQ ID No. 2872 to SEQ ID No. 3891, and coding for a polypeptide of E monocytogenes, as they can be isolated from SΕQ ID No. 690 to 1067, SΕQ ID No. 2049 to SΕQ ID No. 2052 and SΕQ ID No. 2602 to SΕQ ID No. 2871, in particular from SΕQ ID No. 2602 to SΕQ ID No. 2871.

In addition, the nucleotide sequences, characterized in that they comprise a nucleotide sequence chosen from: a) a nucleotide sequence coding for a polypeptide, chosen from the sequences SEQ ID No. 690 to SEQ ID No. 1067, SEQ ID No. 2602 to SEQ ID No. 2871, in particular from SEQ ID No. 2602 to SEQ ID No. 2871; b) a nucleotide sequence comprising at least 75%, 80%, 85%, 90%, 95% or 98% of identity with a nucleotide sequence coding for a polypeptide, chosen from the sequences SEQ ID No. 690 to SEQ ID No 1067, SEQ ID No. 2602 to SEQ ID No. 2871, in particular from SEQ ID No. 2602 to SEQ ID No. 2871; c) a nucleotide sequence hybridizing under high stringency conditions with a nucleotide sequence coding for a polypeptide, chosen from the sequences SEQ ID No. 690 to SEQ ID No. 1067, SEQ ID No. 2602 to SEQ ID No. 2871 , especially from SEQ ID No. 2602 to SEQ ID No. 2871; d) a complementary nucleotide or RNA sequence corresponding to a sequence as defined in a), b) or c); e) a nucleotide sequence of a fragment representative of a sequence as defined in a), b), c) or d); and f) a sequence as defined in a), b), c), d) or e) modified, are also objects of the invention.

The term “nucleic acid, nucleic or nucleic acid sequence, polynucleotide, oligonucleotide, polynucleotide sequence, nucleotide sequence, terms which will be used interchangeably in the present description, is intended to denote a precise sequence of nucleotides, modified or not, making it possible to define a fragment or region of a nucleic acid, which may or may not contain unnatural nucleotides, and which may correspond both to double-stranded DNA, single-stranded DNA and to transcripts of said DNAs. Thus, the nucleic acid sequences according to the invention also include PNA (Peptid Nucleic Acid).

It should be understood that the present invention does not relate to nucleotide sequences in their natural chromosomal environment, that is to say in the natural state. These are sequences which have been isolated and / or purified, that is to say that they have been taken directly or indirectly, for example by copying, their environment having been at least partially modified. This also means the nucleic acids obtained by chemical synthesis.

By "percentage of identity" between two nucleic acid or amino acid sequences within the meaning of the present invention is meant a percentage of identical nucleotides or amino acid residues between the two sequences to compare, obtained after the best alignment, this percentage being purely statistical and the differences between the two sequences being distributed randomly and over their entire length. The term “best alignment” or “optimal alignment” is intended to denote the alignment for which the percentage of identity determined as below is the highest. Sequence comparisons between two nucleic acid or amino acid sequences are traditionally carried out by comparing these sequences after having optimally aligned them, said comparison being carried out by segment or by "comparison window" to identify and compare the regions. sequence similarity locale. The optimal alignment of the sequences for the comparison can be carried out, besides manually, by means of the algorithm of local homology of Smith and Waterman (1981, Ad. App. Math. 2: 482), by means of the algorithm of local homology by Neddleman and Wunsch (1970, J. Mol. Biol. 48: 443), using the similarity search method of Pearson and Lipman (1988, Proc. Natl. Acad. Sci. USA 85: 2444 ), using computer software using these algorithms (GAP, BESTFIT, BLAST P, BLAST N, FASTA and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, WI). In order to obtain optimal alignment, the BLAST program is preferably used with the BLOSUM 62 matrix. The PAM or PAM250 matrices can also be used. The percentage of identity between two nucleic acid or amino acid sequences is determined by comparing these two optimally aligned sequences, the nucleic acid or amino acid sequence to be compared can include additions or deletions by compared to the reference sequence for optimal alignment between these two sequences. The percentage identity is calculated by determining the number of identical positions for which the nucleotide or the amino acid residue is identical in the two sequences, by dividing this number of identical positions by the total number of positions compared and by multiplying the result obtained by 100 to obtain the percentage of identity between these two sequences.

By nucleic acid sequences having a percentage identity of at least 75%, preferably 80%, 85% or 90%, more preferably 95% or even 98%, after optimal alignment with a reference sequence, is meant the nucleic acid sequences having, with respect to the reference nucleic acid sequence, certain modifications such as in particular a deletion, a truncation, an elongation, a chimeric fusion and / or a substitution, in particular punctual, and whose sequence nucleic acid present at least 75%, preferably 80%, 85%, 90%, 95% or 98%, of identity after optimal alignment with the reference nucleic sequence. They are preferably sequences whose complementary sequences are capable of hybridizing specifically with the reference sequences. Preferably, the specific hybridization conditions or high stringency will be such that they ensure at least 75%, preferably 80%, 85%, 90%, 95% or 98% identity after optimal alignment between one of the two sequences and its complementary sequence.

Hybridization under conditions of high stringency means that the conditions of temperature and ionic strength are chosen in such a way that they allow hybridization to be maintained between two complementary DNA fragments. By way of illustration, high stringency conditions of the hybridization step for the purpose of defining the polynucleotide fragments described above are advantageously as follows. DNA-DNA or DNA-RNA hybridization is carried out in two stages: (1) prehybridization at 42 ° C for 3 hours in phosphate buffer (20 mM, pH 7.5) containing 5 x SSC (1 x SSC corresponds to a 0.15 M NaCl + 0.015 M sodium citrate solution), 50% formamide, 7% sodium dodecyl sulfate (SDS), 10 x Denhardt's, 5% dextran sulfate and 1% salmon sperm DNA; (2) actual hybridization for 20 hours at a temperature depending on the size of the probe (ie: 42 ° C, for a probe of size> 100 nucleotides) followed by 2 washes of 20 minutes at 20 ° C in 2 x SSC + 2% SDS, 1 wash for 20 minutes at 20 ° C in 0.1 x SSC + 0.1% SDS. The last washing is carried out in 0.1 x SSC + 0.1% SDS for 30 minutes at 60 ° C. for a probe of size> 100 nucleotides. The high stringency hybridization conditions described above for a polynucleotide of defined size, can be adapted by the skilled person for oligonucleotides of larger or smaller size, according to the teaching of Sambrook et al. (1989, Molecular cloning: a laboratory manual, 2 ^nd Ed. Cold Spring Harbor).

In addition, the term “fragment representative of sequences according to the invention” is intended to denote any nucleotide fragment having at least 15 nucleotides, preferably at least 30, 75, 150, 300 and 450 consecutive nucleotides of the sequence from which it is derived.

By representative fragment is meant in particular a nucleic sequence coding for a biologically active fragment of a polypeptide, as defined below. By representative fragment is also meant the intergenic sequences, and in particular the nucleotide sequences carrying the regulatory signals (promoters, terminators, or even enhancers, etc.).

Among said representative fragments, preference is given to those having nucleotide sequences corresponding to open reading frames, called ORFs sequences (ORFs for "Open Reading Frame"), generally comprised between an initiation codon and a stop codon, or between two stop codons, and coding for polypeptides, preferably at least 100 amino acids, such as for example, without limitation, the ORFs sequences which will be described later. The numbering of the nucleotide sequences ORFs which will be used subsequently in the present description corresponds to the numbering of the amino acid sequences of the proteins encoded by said ORFs.

The representative fragments according to the invention can be obtained for example by specific amplification such as PCR or after digestion with appropriate restriction enzymes of nucleotide sequences according to the invention, this method being described in particular in the work by Sambrook et al. .. Said representative fragments can also be obtained by chemical synthesis when their size is not too large, according to methods well known to those skilled in the art.

Among the sequences containing sequences of the invention, or representative fragments, we also mean the sequences which are naturally framed by sequences which have at least 75%, 80%, 85%, 90%, 95% or 98% d identity with the sequences according to the invention.

By modified nucleotide sequence is meant any nucleotide sequence obtained by mutagenesis according to techniques well known to those skilled in the art, and comprising modifications with respect to the normal sequences, for example mutations in the regulatory and / or promoter sequences of the expression of the polypeptide, in particular leading to a modification of the level of expression or of the activity of said polypeptide.

By modified nucleotide sequence is also meant any nucleotide sequence coding for a modified polypeptide as defined below.

The representative fragments according to the invention can also be probes or primers, which can be used in methods of detection, identification, assay or amplification of nucleic sequences. A probe or primer is defined, within the meaning of the invention, as being a fragment of single-stranded nucleic acids or a denatured double-stranded fragment comprising for example from 12 bases to a few kb, in particular from 15 to a few hundred bases, preferably from 15 to 50 or 100 bases, and having a specificity of hybridization under determined conditions to form a hybridization complex with a target nucleic acid.

The probes and primers according to the invention can be labeled directly or indirectly with a radioactive or non-radioactive compound by methods well known to those skilled in the art, in order to obtain a detectable and / or quantifiable signal (patent FR 78 10975 and bDNA of Chiron EP 225 807 and EP 510 085).

The unlabeled polynucleotide sequences according to the invention can be used directly as a probe or primer.

The sequences are generally marked to obtain sequences which can be used for numerous applications. The labeling of the primers or probes according to the invention is carried out with radioactive elements or with non-radioactive molecules.

Among the radioactive isotopes used, mention may be made of ³² P, ³³ P, ³⁵ S, ³ H or ^{1 25} I. The non-radioactive entities are selected from ligands such as biotin, avidin, streptavidin, dioxygenin, haptens, dyes, luminescent agents such as radioluminescent, chemoluminescent, bioluminescent, fluorescent, phosphorescent agents.

The polynucleotides according to the invention can thus be used as a primer and / or probe in methods using in particular the PCR technique (polymerase chain reaction) (Rolfs et al., 1991, Berlin: Springer-Verlag). This technique requires the choice of pairs of oligonucleotide primers framing the fragment which must be amplified. One can, for example, refer to the technique described in US Pat. No. 4,683,202. The amplified fragments can be identified, for example after agarose or polyacrylamide gel electrophoresis, or after a chromatographic technique such as gel filtration or ion exchange chromatography, and then sequenced. The specificity of the amplification can be controlled by using the nucleotide sequences of polynucleotides of the invention as template, plasmids containing these sequences or even the amplification products derived therefrom. The amplified nucleotide fragments can be used as reagents in hybridization reactions in order to demonstrate the presence, in a biological sample, of a target nucleic acid of sequence complementary to that of said amplified nucleotide fragments.

The invention also relates to the nucleic acids capable of being obtained by amplification using primers according to the invention. Other techniques for amplifying the target nucleic acid can advantageously be used as an alternative to PCR (PCR-like) using pairs of primers of nucleotide sequences according to the invention. By PCR-like is meant to denote all the methods implementing direct or indirect reproductions of the nucleic acid sequences, or in which the labeling systems have been amplified, these techniques are of course known. In general, it is the amplification of DNA by a polymerase; when the original sample is an RNA, a reverse transcription should be carried out beforehand. There are currently many methods for this amplification, such as the SDA technique (Strand Displacement Amplification) or strand displacement amplification technique (Walker et al., 1992, Nucleic Acids Res. 20: 1691), the technique TAS (Transcription-based Amplification System) described by Kwoh et al. (1989, Proc. Natl. Acad. Sci. USA, 86, 1173), the 3SR technique (Self-Sustained Sequence Replication) described by Guatelli et al. (1990, Proc. Natl. Acad. Sci. USA, 87: 1874), the NASBA (Nucleic Acid Sequence Based Amplification) technique described by Kievitis et al. (1991, J. Virol. Methods, 35, 273), the TMA technique (Transcription Mediated Amplification), the LCR technique (Ligase Chain Reaction) described by Landegren et al. (1988, Science 241, 1077), the RCR (Repair Chain Reaction) technique described by Segev (1992, Kessler C. Springer Verlag, Berlin, New York, 197-205), the CPR (Cycling Probe Reaction) technique described by Duck et al. (1990, Biotechniques, 9, 142), the Q-beta-replicase amplification technique described by Miele et al. (1983, J. Mol. Biol., 171, 281). Some of these techniques have since been perfected.

In the case where the target polynucleotide to be detected is an mRNA, it is advantageous to use, prior to the implementation of an amplification reaction using the primers according to the invention or to the implementation of a method detection using the probes of the invention, an enzyme of reverse transcriptase type in order to obtain a cDNA from the mRNA contained in the biological sample. The cDNA obtained will then serve as a target for the primers or probes used in the amplification or detection method according to the invention. The probe hybridization technique can be performed in various ways (Matthews et al., 1988, Anal. Biochem., 169, 1-25). The most general method consists in immobilizing the nucleic acid extracted from cells of different tissues or cells in culture on a support (such as nitrocellulose, nylon, polystyrene) and incubating, under well defined conditions, the target nucleic acid immobilized with the probe. After hybridization, the excess probe is eliminated and the hybrid molecules formed are detected by the appropriate method (measurement of radioactivity, fluorescence or enzymatic activity linked to the probe).

According to another embodiment of the nucleic acid probes according to the invention, the latter can be used as capture probes. In this case, a probe, called a “capture probe”, is immobilized on a support and is used to capture by specific hybridization the target nucleic acid obtained from the biological sample to be tested and the target nucleic acid is then detected. thanks to a second probe, called a “detection probe”, marked by an easily detectable element. Among the nucleic acid fragments of interest, it is thus necessary to cite in particular the antisense oligonucleotides, that is to say those whose structure ensures, by hybridization with the target sequence, an inhibition of the expression of the corresponding product. Mention should also be made of sense oligonucleotides which, by interaction with proteins involved in the regulation of the expression of the corresponding product, will induce either an inhibition or an activation of this expression.

Preferably, the probes or primers according to the invention are immobilized on a support, covalently or non-covalently. In particular, the support can be a DNA chip or a high or medium density filter, also objects of the present invention (patents WO 97/29212, WO 98/27317, WO 97/10365 and WO 92/10588).

The term “DNA chip or high density filter” is intended to denote a support on which DNA sequences are fixed, each of which can be identified by its geographic location. These chips or filters differ mainly in their size, the material of the support, and possibly the number of DNA sequences attached to them.

The probes or primers according to the first invention can be fixed on solid supports, in particular DNA chips, by various manufacturing methods. In particular, a synthesis can be carried out in situ by photochemical addressing or by ink jet. Other techniques consist in carrying out an ex situ synthesis and in fixing the probes on the support of the DNA chip by mechanical, electronic or inkjet addressing. These different methods are well known to those skilled in the art.

A nucleotide sequence (probe or primer) according to the invention therefore allows the detection and / or amplification of specific nucleic sequences. In particular, the detection of these said sequences is facilitated when the probe is fixed to a DNA chip, or to a high density filter.

The use of DNA chips or high density filters makes it possible to determine the expression of genes in an organism having a genomic sequence close to L. monocytogenes or innocua and the typing of the strain in question. The genomic sequence of L. innocua and the partial sequences of L. monocytogenes 4b, supplemented by the identification of the genes of these organisms, as presented in the present invention, serve as a basis for the construction of these DNA chips or filter.

The preparation of these filters or chips consists in synthesizing oligonucleotides, corresponding to the 5 ′ and 3 ′ ends of the genes or to more internal fragments to amplify fragments of a suitable size, for example between approximately 300 and 800 bases. These oligonucleotides are chosen using the genomic sequence and its annotations disclosed by the present invention. The pairing temperature of these oligonucleotides at the corresponding places on the DNA should be approximately the same for each oligonucleotide. This makes it possible to prepare DNA fragments corresponding to each gene by the use of appropriate PCR conditions in a highly automated environment. The amplified fragments are then immobilized on filters or supports in glass, silicon or synthetic polymers and these media are used for hybridization. The availability of such filters and / or chips and of the corresponding annotated genomic sequence makes it possible to study the expression of large sets, or even of all of the genes in the microorganisms associated with Listeria innocua and L. monocytogenes 4b, by preparing the complementary DNAs, and by hybridizing them to the DNA or to the oligonucleotides immobilized on the filters or the chips. Similarly, the filters and / or the chips make it possible to study the variability of the strains or of the species, by preparing the DNA of these organisms and by hybridizing them to the DNA or to the oligonucleotides immobilized on the filters or the chips.

Differences between the genomic sequences of different strains or species can greatly affect the intensity of hybridization and, therefore, disrupt the interpretation of the results. It may therefore be necessary to have the precise sequence of genes of the strain that one wishes to study. The method of detecting genes described later in detail, involving determining the sequence of random fragments of a genome, and organizing them according to the complete genome sequence of L. innocua and L. monocytogenes 4b disclosed herein invention, can be very useful.

The nucleotide sequences according to the invention can be used in DNA chips to carry out the analysis of mutations. This analysis is based on the constitution of chips capable of analyzing each base of a nucleotide sequence according to the invention. In particular, it will be possible to implement micro-sequencing techniques on a DNA chip. The mutations are detected by extension of immobilized primers hybridizing to the matrix of the sequences analyzed, just in position adjacent to that of the mutated nucleotide sought. A single-stranded matrix, RNA or DNA, of the sequences to be analyzed will advantageously be prepared according to conventional methods, from products amplified according to PCR type techniques. The single-stranded DNA or RNA matrices thus obtained are then deposited on the DNA chip, under conditions allowing their specific hybridization to the immobilized primers. A thermostable polymerase, for example Tth or Taq DNA polymerase, specifically extends the 3 'end of the immobilized primer with a labeled nucleotide analog complementary to the nucleotide at the variable site position; for example, thermal cycling is carried out in the presence of fluorescent dideoxyribonucleotides. The experimental conditions will be adapted in particular to the chips used, to the immobilized primers, to the polymerases used, and to the chosen labeling system. An advantage of microsequencing, compared to techniques based on probe hybridization, is that it makes it possible to identify all the variable nucleotides with optimal discrimination under homogeneous reaction conditions; used on DNA chips, it allows optimal resolution and specificity for routine and industrial detection of mutations in multiplex. The use of high density filters and / or chips thus makes it possible to obtain new knowledge on the regulation of genes in organisms of industrial importance, and in particular the listeria propagated under various conditions. It also allows rapid identification of the differences between the genomes of the strains used in multiple industrial applications. In addition, a DNA chip or filter can be an extremely useful tool for the determination, detection and / or identification of a microorganism. Thus, the DNA chips according to the invention are also preferred, which also contain at least one nucleotide sequence of a microorganism other than Listeria monocytogenes 4b or Listeria innocua, immobilized on the support of said chip. Preferably, the microorganism chosen is from bacteria of the genus Listeria (hereinafter designated as bacteria associated with L. monocytogenes), or variants of Listeria monocytogenes EGD-e.

A DNA chip or a filter according to the invention is a very useful element in certain kits or necessary for the detection and / or identification of microorganisms, in particular bacteria belonging to the species Listeria monocytogenes or the associated microorganisms , also objects of the invention.

Furthermore, the DNA chips or filters according to the invention, containing probes or primers specific for Listeria innocua or monocytogenes, are very advantageous elements of kits or necessary for the detection and / or quantification of the expression of genes Listeria innocua or monocytogenes (or associated microorganisms).

Indeed, the control of gene expression is a critical point for optimizing the growth and yield of a strain, either by allowing the expression of one or more new genes, or by modifying the expression of genes already present in the cell. The present invention provides all the naturally active sequences in L. innocua allowing the expression of genes. It thus allows the determination of all the sequences expressed in L. innocua. It also provides a tool for identifying genes whose expression follows a given pattern. To achieve this, the DNA of all or part of the genes of L. innocua and monocytogenes can be amplified using primers according to the invention, then fixed to a support such as for example glass or nylon or a DNA chip, in order to build a tool to monitor the expression profile of these genes. This tool, consisting of this support containing the coding sequences, serves as a hybridization matrix for a mixture of labeled molecules reflecting the messenger RNAs expressed in the cell (in particular the labeled probes according to the invention). By repeating this experiment at different times and combining all of these data with appropriate processing, we then obtain the expression profiles of all of these genes. Knowledge of the sequences which follow a given regulatory scheme can also be used to search in a directed manner, for example by homology, of other sequences following globally, but in a slightly different way the same regulation scheme. In addition, it is possible to isolate each control sequence present upstream of the segments serving as probes and to monitor their activity using an appropriate means such as a reporter gene (luciferase, β-galactosidase, GFP). These isolated sequences can then be modified and assembled by metabolic engineering with sequences of interest with a view to their optimal expression.

The invention also relates to the polypeptides encoded by a nucleotide sequence according to the invention, preferably, by a fragment representative of the preceding sequences and corresponding to an ORF sequence. In particular, the Listeria innocua polypeptides encoded by the sequences SEQ ID No. 12 to SEQ ID No. 689, SEQ ID Nos. 2042 and 2043, SEQ ID Nos. 2047 and 2048, SEQ ID Nos. 2053 to 2056 and SEQ ID Nos. 2059 to 2601, in particular by SEQ ID Nos. 2059 to 2601, or those of Listeria monocytogenes EGDe, characterized in that they are chosen from the polypeptides coded by the sequences SEQ ID No. 690 to SEQ ID No. 1067, SEQ ID No. 2049 to SEQ ID No. 2052 and SEQ ID Nos. 2602 to 2871, notably among SEQ ID Nos. 2602 to 2871, or those of Listeria monocytogenes 4b, characterized in that they are chosen from the polypeptides coded by the sequences SEQ ID No. 3892 to SEQ ID No. 4025, are subject of the invention. The invention also includes the polypeptides characterized in that they comprise a polypeptide chosen from: a) a polypeptide according to the invention; b) a polypeptide having at least 80%, preferably 85%, 90%, 95% and 98% identity with a polypeptide according to the invention; c) a fragment of at least 5 amino acids of a polypeptide according to the invention, or as defined in b); d) a biologically active fragment of a polypeptide according to the invention, or as defined in b) or c); and e) a polypeptide according to the invention, or as defined in b), c) or d) modified. The nucleotide sequences coding for the polypeptides described above are also subject of the invention. In the present description, the terms polypeptides, polypeptide sequences, peptides and proteins are interchangeable. The term polypeptide includes any amino acid sequence used to generate an antibody response. It should be understood that the invention does not relate to polypeptides in natural form, that is to say that they are not taken in their natural environment. On the other hand, it relates to those which could have been isolated or obtained by purification from natural sources, or else obtained by genetic recombination, or by chemical synthesis, and which they can then comprise non-natural amino acids as will be described more far. The term “polypeptide having a certain percentage of identity with another, which will also be designated by homologous polypeptide, is intended to denote the polypeptides having, with respect to the natural polypeptides, certain modifications, in particular a deletion, addition or substitution of at least an amino acid, truncation, elongation, chimeric solution and / or mutation, or polypeptides with post-translational modifications. Among the homologous polypeptides, those whose amino acid sequence have at least 80%, preferably 85%, 90%, 95% and 98% of homology with the amino acid sequences of the polypeptides according to the invention are preferred. . In the case of a substitution, one or more consecutive or non-consecutive amino acids are replaced by “equivalent” amino acids. The expression “equivalent amino acids” is intended here to denote any amino acid capable of being substituted for one of the amino acids of the basic structure without, however, essentially modifying the biological activities of the corresponding peptides as defined by after.

These equivalent amino acids can be determined either on the basis of their structural homology with the amino acids for which they are substituted, or on results of comparative tests of biological activity between the various polypeptides capable of being carried out.

By way of example, mention is made of the possibilities of substitution which may be carried out without it resulting in a thorough modification of the biological activity of the corresponding modified polypeptide. Leucine can thus be replaced by valine or isoleucine, aspartic acid by glutamine acid, glutamine by asparagine, arginine by lysine, etc., the reverse substitutions being naturally possible in the same conditions. The homologous polypeptides also correspond to the polypeptides encoded by the homologous or identical nucleotide sequences, as defined above and thus include, in the present definition, polypeptides which are mutated or correspond to inter or intra species variations, which may exist in Listeria, and which correspond in particular to truncations, substitutions, deletions and / or additions, of at least one amino acid residue.

It is understood that the percentage of identity between two polypeptides is calculated in the same way as between two nucleic acid sequences. Thus, the percentage of identity between two polypeptides is calculated after optimal alignment of these two sequences, over a window of maximum homology. To define said maximum homology window, the same algorithms can be used as for the nucleic acid sequences.

The term “biologically active fragment of a polypeptide according to the invention” is intended to denote in particular a fragment of polypeptide, as defined below, having at least one of the biological characteristics of the polypeptides according to the invention, in particular in that it is able to exercise in general even partial activity, such as for example:

- an enzymatic (metabolic) activity or an activity which may be involved in the biosynthesis or biodegradation of organic or inorganic compounds;

- structural activity (cell envelope, chaperone molecule, ribosome);

- a transport activity (energy, ion); or in protein secretion;

- an activity in the process of replication, amplification, preparation, transcription, translation or maturation, in particular of DNA, RNA or proteins.

The term “polypeptide fragment according to the invention” is intended to denote a polypeptide comprising at least 5 amino acids, preferably 10, 15, 25, 50, 100 and 150 amino acids. The fragments of polypeptides can correspond to fragments isolated or purified naturally present in the strains of Listeria, or to fragments which can be obtained by cleavage of said polypeptide by a proteolitic enzyme such as trypsin or chymotrypsin or collagenase, by a reagent chemical (cyanogen bromide, CNBr) or by placing said polypeptide in a very acidic environment (for example at pH = 2.5). Polypeptide fragments can also be prepared by chemical synthesis, from hosts transformed by an expression vector according to the invention which contain a nucleic acid allowing the expression of said fragment, and placed under the control of regulatory elements and / or appropriate expression.

The term “modified polypeptide” of a polypeptide according to the invention is intended to denote a polypeptide obtained by genetic recombination or by chemical synthesis as described below, which exhibits at least one modification with respect to the normal sequence. These modifications can be carried in particular on amino acids necessary for the specificity or the efficiency of the activity, or at the origin of the structural conformation, of the charge, or of the hydrophobicity of the polypeptide according to the invention. It is thus possible to create polypeptides of equivalent, increased or decreased activity, or of equivalent specificity, narrower or wider. Among the modified polypeptides, mention should be made of the polypeptides in which up to five amino acids can be modified, truncated at the N or C-terminus, or else deleted, or added.

As indicated, the modifications of a polypeptide are aimed in particular at:

- to allow its implementation in processes of biosynthesis or biodegradation of organic or inorganic compounds,

- to allow its implementation in replication, amplification, repair and rules for transcription, translation, or maturation, in particular of DNA, RNA, or proteins,

- to allow its improved secretion, - to modify its solubility, the efficiency or the specificity of its activity, or to facilitate its purification.

Chemical synthesis also has the advantage of being able to use unnatural amino acids or non-peptide bonds. Thus, it may be advantageous to use unnatural amino acids, for example in D form, or analogs of amino acids, in particular suffering forms.

The present invention provides the nucleotide sequence of the Listeria innocua genome and the partial sequence of Listeria monocytogenes serotype 4b, as well as certain polypeptide sequences. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the biosynthesis of amino acids. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the biosynthesis of cofactors, prosthetic groups and transporters .

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a cell envelope polypeptide or present on the surface of Listeria innocua or monocytogenes 4b or for one of its fragments.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the cellular machinery.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the central intermediate metabolism. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in energy metabolism.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the metabolism of fatty acids and phospholipids.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the metabolism of nucleotides, purines, pyrimidines or nucleosides.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the regulatory functions. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria innocua or monocytogenes 4b polypeptide or one of its fragments involved in the replication process.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria innocua or monocytogenes 4b polypeptide or one of its fragments involved in the transcription process.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria innocua or monocytogenes 4b polypeptide or one of its fragments involved in the translation process. Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the protein transport and binding process .

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a Listeria innocua or monocytogenes 4b polypeptide or one of its fragments involved in the adaptation to atypical conditions.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments in the sensitivity to drugs and the like.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the functions relating to transposons.

Preferably, the invention relates to a nucleotide sequence according to the invention, characterized in that it codes for a specific polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in biosynthesis amino acids.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a Listeria polypeptide innocua or monocytogenes 4b or one of its fragments involved in the biosynthesis of cofactors, prosthetic groups and transporters.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a cell envelope or surface polypeptide of Listeria innocua or monocytogenes 4b or a of its fragments.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the machinery cellular.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in metabolism central intermediary. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in metabolism energy.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in metabolism fatty acids and phospholipids.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in metabolism nucleotides, purines, pyrimidines or nucleosides.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the functions of regulation.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the process replication. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the process of transcription. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the process translation.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the process protein transport and binding.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the adaptation to atypical conditions.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments in sensitivity to drugs and the like.

In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments involved in the functions relating to transposons. In another aspect, preferably, the invention relates to a polypeptide according to the invention, characterized in that it is a specific polypeptide of Listeria innocua or monocytogenes 4b or one of its fragments.

It is important to note, however, that a living organism is a whole and must be taken as such. Thus, in order to be able to develop and exhibit its properties, any organism needs interactions between the different metabolic pathways. Thus, the classification set out above should not be considered as limiting, a gene which may be involved in two distinct metabolic pathways.

The present invention also relates to the nucleotide and / or polypeptide sequences according to the invention, characterized in that said sequences are recorded on a recording medium whose shape and nature facilitate the reading, analysis and / or exploitation of said sequence (s). These supports can also contain other information extracted from the present invention, in particular analogies with already known sequences, and / or information concerning the nucleotide sequences and / or polypeptides of other microorganisms in order to facilitate the comparative analysis and the exploitation of the results obtained. Among these recording media, particular preference is given to media readable by a computer, such as magnetic, optical, electrical or hybrid media, in particular computer floppy disks, CD-ROMs, computer servers. Such recording media are also subject of the invention.

The recording media according to the invention, with the information provided, are very useful for the choice of primers or nucleotide probes for the determination of genes in Listeria innocua or monocytogenes 4b or strains close to this organism. Likewise, the use of these supports for the study of the genetic polymorphism of strains close to Listeria irmocua or monocytogenes 4b, in particular by the determination of the regions of collinearity, is very useful insofar as these supports provide not only the sequence nucleotide of the genome of Listeria innocua or monocytogenes 4b, but also the genomic organization in said sequence. Thus, the uses of recording media according to the invention are also objects of the invention.

The analysis of homology between different sequences is in fact advantageously carried out using sequence comparison software, such as the Blast software, or the software of the GCG kit, described above.

The invention also relates to the cloning and / or expression vectors, which contain a nucleotide sequence according to the invention.

The vectors according to the invention preferably comprise elements which allow the expression and / or the secretion of the nucleotide sequences in a determined host cell.

The vector must then include a promoter, translation initiation and termination signals, as well as suitable regions for transcription regulation. It must be able to be maintained stably in the host cell and may possibly have specific signals which specify the secretion of the translated protein. These various elements are chosen and optimized by a person skilled in the art according to the cell host used. To this end, the nucleotide sequences according to the invention can be inserted into vectors with autonomous replication within the chosen host, or be integrative vectors of the chosen host.

Such vectors are prepared by methods commonly used by those skilled in the art, and the resulting clones can be introduced into an appropriate host by standard methods, such as lipofection, electroporation, heat shock, or chemical methods .

The vectors according to the invention are for example vectors of plasmid or viral origin. They are useful for transforming host cells in order to clone or express the nucleotide sequences according to the invention. The invention also includes host cells transformed with a vector according to the invention.

The cell host can be chosen from prokaryotic or eukaryotic systems, for example bacterial cells but also yeast cells or animal cells, in particular mammalian cells. You can also use insect cells or plant cells. The preferred host cells according to the invention are in particular prokaryotic cells, preferably bacteria belonging to the genus Listeria, to the species Listeria innocua or monocytogenes 4b, or the microorganisms associated with the species Listeria innocua or monocytogenes 4b. The invention also relates to plants and animals, except humans, which comprise a transformed cell according to the invention. The cells transformed according to the invention can be used in processes for the preparation of recombinant polypeptides according to the invention. The methods for preparing a polypeptide according to the invention in recombinant form, characterized in that they use a vector and / or a cell transformed with a vector according to the invention are themselves included in the present invention. Preferably, a cell transformed with a vector according to the invention is cultivated under conditions which allow the expression of said polypeptide and said recombinant peptide is recovered.

As has been said, the cell host can be chosen from prokaryotic or eukaryotic systems. In particular, it is possible to identify nucleotide sequences according to the invention, facilitating secretion in such a prokaryotic or eukaryotic system. A vector according to the invention carrying such a sequence can therefore be advantageously used for the production of recombinant proteins, intended to be secreted. Indeed, the purification of these recombinant proteins of interest will be facilitated by the fact that they are present in the supernatant of the cell culture rather than inside the host cells.

The polypeptides according to the invention can also be prepared by chemical synthesis. Such a preparation process is also an object of the invention. A person skilled in the art knows the chemical synthesis processes, for example the techniques implementing solid phases (see in particular Steward et al., 1984, Solid phase peptides synthesis, Pierce Chem. Company, Rockford, 11 1, 2nd ed. , (1984)) or techniques using partial solid phases, by condensation of fragments or by synthesis in conventional solution. The polypeptides obtained by chemical synthesis and which may contain corresponding unnatural amino acids are also included in the invention.

The invention further relates to hybrid polypeptides having at least one polypeptide or a fragment thereof according to the invention, and a sequence of a polypeptide capable of inducing an immune response in humans or animals. Advantageously, the antigenic determinant is such that it is capable of inducing a humoral and / or cellular response.

Such a determinant may comprise a polypeptide or one of its fragments according to the invention in glycosylated form used with a view to obtaining immunogenic compositions capable of inducing the synthesis of antibodies directed against multiple epitopes. Said polypeptides or their glycosylated fragments also form part of the invention.

These hybrid molecules can consist in part of a molecule carrying polypeptides or their fragments according to the invention, associated with a possibly immunogenic part, in particular an epitope of diphtheria toxin, tetanus toxin, a surface antigen of the virus. hepatitis B (patent FR 79 2181 1), the VP1 antigen of the poliomyelitis virus or any other toxin or viral or bacterial antigen.

The methods of synthesis of the hybrid molecules include the methods used in genetic engineering to construct hybrid nucleotide sequences coding for the polypeptide sequences sought. We can, for example, advantageously refer to the technique for obtaining genes coding for fusion proteins described by Minton in 1984.

Said hybrid nucleotide sequences coding for a hybrid polypeptide as well as the hybrid polypeptides according to the invention characterized in that they are Recombinant polypeptides obtained by the expression of said hybrid nucleotide sequences, also form part of the invention.

The invention also includes the vectors characterized in that they contain one of said hybrid nucleotide sequences. Host cells transformed by said vectors, transgenic animals comprising one of said transformed cells as well as methods for preparing recombinant polypeptides using said vectors, said transformed cells and / or said transgenic animals also form part of the invention.

The coupling between a polypeptide according to the invention and an immunogenic polypeptide can be carried out chemically, or biologically. Thus, according to the invention, it is possible to introduce one or more binding element (s), in particular amino acids to facilitate the coupling reactions between the polypeptide according to the invention, and the immunostimulatory polypeptide, the covalent coupling of the immunostimulatory antigen can be produced at the N or C-terminal end of the polypeptide according to the invention. The bifunctional reagents allowing this coupling are determined as a function of the end chosen to achieve this coupling, and the coupling techniques are well known to those skilled in the art.

The conjugates resulting from a coupling of peptides can also be prepared by genetic recombination. The hybrid (conjugated) peptide can in fact be produced by recombinant DNA techniques, by insertion or addition to the DNA sequence coding for the polypeptide according to the invention, of a sequence coding for the peptide (s) ) antigen (s), immunogen (s) or hapten (s). These techniques for preparing hybrid peptides by genetic recombination are well known to those skilled in the art (see for example Makrides, 1996, Microbiological Reviews 60, 512-538). Preferably, said immune polypeptide is chosen from the group of peptides containing toxoids, in particular the diphtheria toxoid or the tetanus toxoid, proteins derived from Streptococcus (such as the protein for binding to human seralbumin), OMPA membrane proteins and complexes. proteins from external membranes, vesicles from external membranes or thermal shock proteins.

The hybrid polypeptides according to the invention are very useful for obtaining monoclonal or polyclonal antibodies capable of specifically recognizing the polypeptides according to the invention. Indeed, a hybrid polypeptide according to the invention allows the potentiation of the immune response, against the polypeptide according to the invention coupled to the immunogenic molecule. Such monoclonal or polyclonal antibodies, their fragments, or chimeric antibodies, recognizing the polypeptides according to the invention, are also objects of the invention.

The specific monoclonal antibodies can be obtained according to the conventional method of hybridoma culture described by Kohler and Milstein (1975, Nature 256, 495).

The antibodies according to the invention are for example chimeric antibodies, humanized antibodies, Fab fragments, or F (ab '). They can also be in the form of immunoconjugates or labeled antibodies in order to obtain a detectable and / or quantifiable signal.

Thus, the antibodies according to the invention can be used in a method for the detection and / or identification of bacteria belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism in a biological sample, characterized in that that it comprises the following stages: a) bringing the biological sample into contact with an antibody according to the invention; b) highlighting of the antigen-antibody complex possibly formed. The antibodies according to the present invention can also be used in order to detect an expression of a gene of Listeria innocua or monocytogenes 4b or of associated microorganisms. Indeed, the presence of the expression product of a gene recognized by an antibody specific for said expression product can be detected by the presence of an antigen-antibody complex formed after contacting the strain of Listeria innocua or monocytogenes 4b or of the microorganism associated with an antibody according to the invention. The bacterial strain used may have been "prepared", that is to say centrifuged, lysed, placed in a reagent suitable for constituting the medium suitable for the immunological reaction. In particular, a method of detecting expression in the gene, corresponding to a Western blot, which can be carried out after an electrophoresis on polyacrylamide gel of a lysate of the bacterial strain, is preferred, in the presence or in the absence of reducing conditions (SDS-PAGE). After migration and separation of the proteins on the polyacrylamide gel, said proteins are transferred to an appropriate membrane (for example made of nylon) and the presence of the protein or polypeptide of interest is detected, by contacting said membrane with a antibody according to the invention. Thus, the present invention also comprises the kits or kits necessary for the implementation of a method as described (for detecting the expression of a gene for

Listeria innocua or monocytogenes 4b or an associated microorganism, or for the detection and / or identification of bacteria belonging to the species Listeria innocua or monocytogenes 4b or an associated microorganism), comprising the following elements: a ) a polyclonal or monoclonal antibody according to the invention; b) optionally, the reagents for constituting the medium suitable for the immunological reaction; c) optionally, the reagents allowing the detection of the antigen-antibody complexes produced by the immunological reaction.

The polypeptides and antibodies according to the invention can advantageously be immobilized on a support, in particular a protein chip. Such a protein chip is an object of the invention, and may also contain at least one polypeptide from a microorganism other than Listeria innocua or monocytogenes 4b or an antibody directed against a compound of a microorganism other than Listeria innocua or monocytogenes 4b.

The protein chips or high density filters containing proteins according to the invention can be constructed in the same way as the DNA chips according to the invention. In practice, it is possible to carry out the synthesis of the polypeptides directly fixed on the protein chip, or to carry out an ex situ synthesis followed by a step of fixing on the said chip the synthesized polypeptide. The latter method is preferable, when it is desired to attach proteins of large size to the support, these proteins being advantageously prepared by genetic engineering. However, if it is desired to fix only peptides on the support of said chip, it may be more advantageous to synthesize said peptides directly in situ.

The protein chips according to the invention can advantageously be used in kits or necessary for the detection and / or identification of bacteria associated with the species Listeria innocua or monocytogenes 4b or with a microorganism, or more generally in kits or kits for the detection and / or identification of microorganisms. When the polypeptides according to the invention are fixed on the DNA chips, the presence of antibodies is sought in the samples tested, the fixing of an antibody according to the invention on the support of the protein chip allowing the identification of the protein of which said antibody is specific. Preferably, an antibody according to the invention is fixed on the support of the protein chip, and the presence of the corresponding antigen, specific for Listeria innocua or monocytogenes 4b or an associated microorganism, is detected.

A protein chip described above can be used for the detection of gene products, to establish an expression profile of said genes, in addition to a DNA chip according to the invention.

The protein chips according to the invention are also extremely useful for proteomics experiments, which studies the interactions between the different proteins of a given microorganism. In a simplified manner, peptides representative of the various proteins of an organism are fixed on a support. Then, said support is brought into contact with labeled proteins, and after an optional rinsing step, interactions between said labeled proteins and the peptides fixed on the protein chip are detected.

Thus, protein chips comprising a polypeptide sequence according to the invention or an antibody according to the invention are subject of the invention, as well as the kits or kits containing them.

The present invention also covers a method for detecting and / or identifying bacteria belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism in a biological sample, which implements a nucleotide sequence according to the invention .

It should be understood that the teπne biological sample relates in the present invention to the samples taken from a living organism (in particular blood, tissues, organs or others taken from a mammal) or a sample containing biological material, that is, DNA or RNA. Such a biological sample also includes food compositions containing bacteria (for example cheeses, dairy products), but also food compositions containing yeasts (beers, breads) or others. The third biological sample also relates to the bacteria isolated from these samples or food compositions. The detection and / or identification process using the nucleotide sequences according to the invention can be of various nature.

A method is preferred comprising the following steps: a) optionally, isolation of the DNA from the biological sample to be analyzed, or obtaining a cDNA from the RNA of the biological sample; b) specific amplification of the DNA of bacteria belonging to the species Listeria innocua or monocytogenes 4b or to a microorganism associated with the aid of at least one primer according to the invention; c) highlighting of the amplification products. This process is based on specific amplification of DNA, in particular by an amplification chain reaction.

A method is also preferred comprising the following steps: a) bringing a nucleotide probe according to the invention into contact with a biological sample, the nucleic acid contained in the biological sample having, if necessary, previously been made accessible to hybridization, under conditions allowing hybridization of the probe to the nucleic acid of a bacterium belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism; b) demonstration of the hybrid possibly formed between the nucleotide probe and the DNA of the biological sample. Such a method should not be limited to the detection of the presence of the DNA contained in the biological sample to be tested, it can also be implemented to detect the RNA contained in said sample. This process includes in particular the Southern and Northern blot.

Another preferred method according to the invention comprises the following steps: a) bringing a nucleotide probe immobilized on a support according to the invention into contact with a biological sample, the nucleic acid of the sample, having, where appropriate , been previously made accessible for hybridization, under conditions allowing hybridization of the probe to the nucleic acid of a bacterium belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism; b) bringing the hybrid formed into contact between the nucleotide probe immobilized on a support and the nucleic acid contained in the biological sample, where appropriate after elimination of the DNA from the biological sample which has not hybridized with the probe, with a labeled nucleotide probe according to the invention; c) highlighting of the new hybrid formed in step b). This method is advantageously used with a DNA chip according to the invention, the desired nucleic acid hybridizing with a probe present on the surface of said chip, and being detected by the use of a labeled probe. This process is advantageously implemented by combining a prior step of amplification of DNA or complementary DNA optionally obtained by reverse transcription, using primers according to the invention.

Thus, the present invention also includes kits or kits for the detection and / or identification of bacteria belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism, characterized in that it comprises the following elements : a) a nucleotide probe according to the invention; b) optionally, the reagents necessary for carrying out a hybridization reaction; c) optionally, at least one primer according to the invention as well as the reagents necessary for a DNA amplification reaction.

Likewise, the present invention also includes kits or kits for the detection and / or identification of bacteria belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism, characterized in that it comprises the elements following: a) a nucleotide probe, called capture probe, according to the invention; b) an oligonucleotide probe, called the revelation probe, according to the invention; c) optionally, at least one primer according to the invention as well as the reagents necessary for a DNA amplification reaction. Finally, the kits or kits for the detection and / or identification of bacteria belonging to the species Listeria innocua or monocytogenes 4b or to an associated microorganism, characterized in that it comprises the following elements: a) at least a primer according to the invention; b) optionally, the reagents necessary to carry out a DNA amplification reaction; c) optionally, a component making it possible to verify the sequence of the amplified fragment, more particularly an oligonucleotide probe according to the invention, are also objects of the present invention.

Preferably, said primers and / or probes and / or polypeptides and / or antibodies according to the present invention used in the methods and / or kits or necessary according to the present invention are chosen from primers and / or probes and / or polypeptides and / or antibodies specific for the species Listeria innocua or monocytogenes 4b. Preferably, these elements are chosen from the nucleotide sequences coding for a secreted protein, among secreted polypeptides, or among antibodies directed against secreted polypeptides of Listeria innocua or monocytogenes 4b.

The present invention also relates to strains of Listeria innocua or monocytogenes 4b and / or associated microorganisms containing one or more mutation (s) in a nucleotide sequence according to the invention, in particular an ORF sequence, or their regulatory elements. (in particular promoters).

According to the present invention, the strains of Listeria innocua or monocytogenes 4b are preferred, having one or more mutation (s) in the nucleotide sequences coding for polypeptides involved in the cellular machinery, in particular secretion, central intermediate metabolism, metabolism. energy, the processes of amino acid synthesis, transcription and translation, synthesis of polypeptides.

Said mutations can lead to inactivation of the gene, or in particular when they are located in the regulatory elements of said gene, to overexpression of the latter.

The invention further relates to the use of a nucleotide sequence according to the invention, a polypeptide according to the invention, an antibody according to the invention, a cell according to the invention, and / or d '' an animal transformed according to the invention, for the selection of organic or inorganic compound capable of modulating, regulating, inducing or inhibiting the expression of genes, and / or modifying the cellular replication of eukaryotic or prokaryotic cells or capable of inducing, inhibiting or aggravating the pathologies linked to infection with Listeria innocua or monocytogenes 4b or one of its associated microorganisms.

The invention also includes a method of selecting compounds capable of binding to a polypeptide or a fragment thereof according to the invention, capable of binding to a nucleotide sequence according to the invention, or capable of recognizing an antibody according to claim , and / or capable of modulating, regulating, inducing or inhibiting gene expression, and / or modifying the growth or cellular replication of eukaryotic or prokaryotic cells, or capable of inducing, inhibiting or aggravate in an animal or human organism the pathologies linked to an infection by Listeria, for example by L. monocytogenes 4b, or one of its associated microorganisms, characterized in that it comprises the following stages: a) bringing said compound into contact with said polypeptide, said nucleotide sequence, with a cell transformed according to the invention and / or administering said compound to an animal transformed according to the invention; b) determining the capacity of said compound to bind with said polypeptide or said nucleotide sequence, or to modulate, regulate, induce or inhibit the expression of genes, or to modulate cell growth or replication, or induce, inhibit or aggravate in said transformed animal the pathologies linked to an infection with Listeria, for example L. monocytogenes 4b or one of its associated microorganisms. The cells and / or animals transformed according to the invention can advantageously serve as a model and be used in methods for studying, identifying and / or selecting compounds capable of being responsible for pathologies induced or aggravated by Listeria monocytogenes, or susceptible to prevent and / or treat these pathologies. In particular, the transformed host cells, in particular bacteria of the Listeria family, the transformation of which by a vector according to the invention can, for example, increase or inhibit its infectious power, or modulate the pathologies usually induced or aggravated by the infection, may be used to infect animals whose pathologies will be monitored. These unprocessed animals, infected for example with transformed Listeria bacteria, could serve as a study model. Likewise, the animals transformed according to the invention may be used in methods of selecting compounds capable of preventing and / or treating diseases due to Listeria. Said methods using said transformed cells and / or transformed animals form part of the invention. The compounds which can be selected can be organic compounds such as polypeptides or carbohydrates or any other organic or inorganic compounds already known, or new organic compounds produced from molecular modeling techniques and obtained by chemical or biochemical synthesis. , these techniques being known to those skilled in the art. Said selected compounds may be used to modulate the growth and / or cell replication of Listeria innocua or monocytogenes 4b or any other associated microorganism and thus to control infection by these microorganisms. Said compounds according to the invention may also be used to modulate the growth and / or cell replication of all eukaryotic or prokaryotic cells, in particular tumor cells and infectious microorganisms, for which said compounds will prove to be active, the methods making it possible to determine said modulations being well known to those skilled in the art.

The term “compound capable of modulating the growth of a microorganism” is intended to denote any compound which makes it possible to intervene, modify, limit and / or reduce the development, growth, rate of proliferation and / or viability of said microorganism. .

This modulation can be carried out for example by an agent capable of binding to a protein and thus of inhibiting or potentiating its biological activity, or capable of binding to a membrane protein of the external surface of a microorganism and of block the penetration of said microorganism into the host cell or promote the action of the immune system of the infected organism directed against said microorganism. This modulation can also be carried out by an agent capable of binding to a nucleotide sequence of a DNA or RNA of a microorganism and of blocking for example the expression of a polypeptide whose biological or structural activity is necessary to the growth or reproduction of said microorganism.

The term “associated microorganism” is intended to denote any microorganism whose gene expression can be modulated, regulated, induced or inhibited, or whose cell growth or replication can also be modulated by a compound of l 'invention. The term “associated microorganism in the present invention” is also intended to denote any microorganism comprising nucleotide sequences or polypeptides according to the invention. These microorganisms may in certain cases contain polypeptides or nucleotide sequences identical or homologous to those of the invention and may also be detected and / or identified by the methods or kit for detection and / or identification according to the invention and also serve as a target for the compounds of the invention. The term “microorganism” is also intended to denote any Listeria monocytogenes microorganism of any serotype.

The invention relates to compounds capable of being selected by a selection method according to the invention.

The invention also relates to a pharmaceutical composition comprising a compound chosen from the following compounds: a) a nucleotide sequence according to the invention; b) a polypeptide according to the invention; c) a vector according to the invention; d) an antibody according to the invention; and e) a compound capable of being selected by a selection method according to the invention, optionally in combination with a pharmaceutically acceptable vehicle.

The term effective quantity is intended to denote a sufficient quantity of the said compound or antibody, or of the polypeptide of the invention, making it possible to modulate the growth of Listeria innocua or monocytogenes 4b or of an associated microorganism.

The invention also relates to a pharmaceutical composition according to the invention for the prevention or treatment of an infection by a bacterium belonging to the genus Listeria or by an associated microorganism.

The invention further relates to an immunogenic and / or vaccine composition, characterized in that it comprises one or more polypeptides according to the invention and / or one or more hybrid polypeptides according to the invention. The invention also includes the use of a transformed cell according to the invention, for the preparation of a vaccine composition.

The invention also relates to a vaccine composition, characterized in that it contains a nucleotide sequence according to the invention, a vector according to the invention and / or a transformed cell according to the invention. The invention also relates to the vaccine compositions according to the invention, for the prevention or treatment of an infection by a bacterium belonging to the genus Listeria or by an associated microorganism.

Preferably, the immunogenic and / or vaccine compositions according to the invention intended for the prevention and / or treatment of infection by Listeria or by an associated microorganism will be chosen from the immunogenic and / or vaccine compositions comprising a polypeptide or one of its fragments corresponding to a protein, or one of its fragments, of the Listeria cell envelope. The vaccine compositions comprising nucleotide sequences will preferably also comprise nucleotide sequences coding for a polypeptide or one of its fragments corresponding to a protein, or one of its fragments, of the cell envelope of Listeria.

The polypeptides of the invention or their fragments entering into the immunogenic compositions according to the invention can be selected by techniques known to those skilled in the art, for example on the capacity of said polypeptides to stimulate the T cells, which results, for example, in their proliferation or the secretion of interleukins, and which results in the production of antibodies directed against said polypeptides.

In mice, in which a weight dose of the vaccine composition comparable to the dose used in humans is administered, the antibody reaction is tested by sampling the serum followed by a study of the formation of a complex between the antibodies present in the serum and the antigen of the vaccine composition, according to the usual techniques.

According to the invention, said vaccine compositions will preferably be in association with a pharmaceutically acceptable vehicle and, where appropriate, with one or more appropriate immunity adjuvants.

Today, various types of vaccines are available to protect humans against infectious diseases: live attenuated microorganisms (M. bovis - BCG for tuberculosis), inactivated microorganisms (influenza virus), cell-free extracts (Bordetella pertussis for pertussis), recombinant proteins (hepatitis B virus surface antigen), polysaccharides (pneumococci). Vaccines prepared from synthetic peptides or genetically modified microorganisms expressing heterologous antigens are being tested. Even more recently, recombinant plasmid DNAs carrying genes coding for protective antigens have been proposed as an alternative vaccine strategy. This type of vaccination is carried out with a particular plasmid derived from an E. coli plasmid which does not replicate in vivo and which codes only for the vaccinating protein. Animals have been immunized by simply injecting naked plasmid DNA into the muscle. This technique leads to the expression of the vaccine protein in situ and to an immune response of cell type (CTL) and of humoral type (antibody). This double induction of the immune response is one of the main advantages of the vaccination technique with naked DNA.

The vaccine compositions comprising nucleotide sequences or vectors into which said sequences are inserted, are in particular described in international application No. WO 90/1 1092 and also in international application No. WO 95/1 1307.

The nucleotide sequence constituting the vaccine composition according to the invention can be injected into the host after being coupled to compounds which promote the penetration of this polynucleotide inside the cell or its transport to the cell nucleus. The resulting conjugates can be encapsulated in polymer microparticles, as described in international application No. WO 94/27238 (Medisorb Technologies International).

According to another embodiment of the vaccine composition according to the invention, the nucleotide sequence, preferably DNA, is complexed with DEAE-dextran, with nuclear proteins, with lipids or encapsulated in liposomes or even introduced under the form of a gel facilitating its transfection into cells. The polynucleotide or the vector according to the invention can also be in suspension in a buffer solution or be associated with liposomes. Advantageously, such a vaccine will be prepared according to the technique described by Tacson et al. or Huygen et al. in 1996 or according to the technique described by Davis et al. in international application No. WO 95/1 1307.

Such a vaccine can also be prepared in the form of a composition containing a vector according to the invention, placed under the control of regulatory elements allowing its expression in humans or animals. It is possible, for example, to use, as a vector for the in vivo expression of the polypeptide antigen of interest, the plasmid pcDNA3 or the plasmid pcDNAl / neo, both marketed by Invitrogen (R & D Systems, Abingdon, United Kingdom). United). Such a vaccine will advantageously comprise, in addition to the recombinant vector, a saline solution, for example a sodium chloride solution.

The term “pharmaceutically acceptable vehicle” is intended to denote a compound or a combination of compounds entering into a pharmaceutical or vaccine composition which does not cause side reactions and which allows for example the facilitation of the administration of the active compound, the increase in its duration of life and / or its efficiency in the organism, increasing its solubility in solution or improving its conservation. These pharmaceutically acceptable vehicles are well known and will be adapted by those skilled in the art depending on the nature and the mode of administration of the active compound chosen.

Regarding vaccine formulations, these can include suitable immunity adjuvants which are known to those skilled in the art, such as, for example, aluminum hydroxide, a representative of the family of muramyl peptides. as one of the peptide derivatives of N-acetyl-muramyl, a bacterial lysate, or even the incomplete adjuvant of Freund. Preferably, these compounds will be administered by the systemic route, in particular by the intravenous route, by the intramuscular, intradermal or subcutaneous route, or by the oral route. More preferably, the vaccine composition comprising polypeptides according to the invention will be administered several times, over a period of time, by the intradermal or subcutaneous route.

Their optimal methods of administration, dosages and dosage forms can be determined according to the criteria generally taken into account in establishing a treatment adapted to a patient such as, for example, the patient's age or body weight, the severity of his general condition, tolerance to treatment and side effects observed.

Finally, the invention comprises the use of a composition according to the invention, for the treatment or prevention of diseases induced or aggravated by the presence of Listeria.

Furthermore, the present invention also relates to a genomic DNA library of a bacterium of the genus Listeria, preferably, Listeria innocua or monocytogenes, preferably the strain 4b.

The genomic DNA banks described in the present invention, in particular the Li-shotgun bank deposited at the CNCM on October 2, 2000 under order number n ° 1-2565 and the Lmb4b-shotgun bank deposited at the CNCM on 2 October 2000 under the serial number n ° 1-2566, cover the genome of Listeria innocua and Listeria monocytogenes 4b respectively. However, although certain regions could not be cloned into said library, due to lethality problems in Escherichia coli, these regions can easily be amplified and identified by a person skilled in the art, using oligonucleotides specific for the sequences of the sequences. ends of the different clones which form the contigs.

The present invention also relates to methods for the isolation of a polynucleotide of interest present in a strain of Listeria and absent in another strain, which uses at least one DNA library based for example on a plasmid pcDNA2.1 containing the Listeria genome. The method according to the invention for the isolation of a polynucleotide of interest can comprise the following steps: a) isolating at least one polynucleotide contained in a clone of the DNA library of Listeria origin, b) isolating: at least one genomic polynucleotide or cDNA of a listeria, said listeria belonging to a strain different from the strain used for the construction of the DNA library of step a) or, alternatively,

- at least one polynucleotide contained in a clone of a DNA library prepared from the genome of a Listeria which is different from the Listeria used for the construction of the DNA library of step a); c) hybridizing the polynucleotide of step a) to the polynucleotide of step b); d) selecting the polynucleotides of step a) which have not formed a hybridization complex with the polynucleotides of step b); e) characterize the selected polynucleotide.

The polynucleotide of step a) can be prepared by digestion of at least one recombinant clone with an appropriate restriction enzyme, and optionally, the amplification of the resulting polynucleotide insert.

Thus, the method of the invention allows a person skilled in the art to carry out comparative genomic studies between the different strains or species of the genus

Listeria, for example between pathogenic strains and their non-pathogenic equivalents.

In particular, it is possible to study and determine the regions of polymorphism between said strains.

EXAMPLES

1. Construction of banks

The chromosomal DNA of the strains studied was prepared by a conventional method including treatment with proteinase K and phenol extraction (Jacquet, C, et al., Zentralbl Bakteriol., 276: 356-365, 1992). About 10 µg of DNA was broken by nebulization (1 minute at 1 bar pressure) (Buchrieser, C, et al., Infect.

Immun., 67: 4851 -4861, 1999). The ends of the DNA fragments were made blunt by causing the bacteriophage T4 DNA polymerase to act for 15 minutes.

37 ° C in the presence of the 4 tri-phosphate nucleotides. The enzyme was inactivated by a 15 min incubation at 75 ° C. Adapters (invitrogen Cat. No. 408-18) were then ligated at these ends. After ligation, the chromosomal DNA fragments having a size between 1000 and 3000 base pairs were purified after electrophoresis on agarose gel. The vector used for the construction of the bank, pcDNA2.1

(Invitrogen), was digested with the enzyme BstXl and purified by geneclean (BIO-101) after agarose gel electrophoresis. The chromosomal DNA and the purified vector were ligated by the action of the bacteriophage T4 ligase. The ligation mixture was introduced by transformation into the strain of Escherichia coli XL2-blue (Stratagene). About 4000 colonies are obtained per µl of the ligation mixture. This process is used to build the Li-shotgun bank deposited at the CNCM on October 2, 2000 under the n ° 1-2565 for the Listeria innocua strain (CLIP 1 1262) and the Lm4b-shotgun bank deposited at the CNCM on October 2, 2000 under No. 1-2566 for the strain Listeria monocytogenes serotype 4b (CLIP 80459).

2. Preparation of the plasmids and sequencing The plasmids were prepared by a semi-automatic preparation method developed in the GMP laboratory (Genomics of Pathogenic Microorganisms of the Institut Pasteur) based on the alkaline lysis method (Birnboim, H. C , Methods Enzymol., 100: 243-255, 1983). The chromosomal inserts were sequenced from their two ends using the T7 and universal primer following the recommendations of the supplier (PE-biosystems). The sequences were determined using automatic sequencers of type 377 and 3700 (PE-Biosystem).

3. Assembling the sequences

The sequences were assembled using the software package developed at the University of Washington, Phred, Phrap and Consed (Ewing, B., et al., Génome Res., 8: 186-194, 1998; Gordon, D ., et al., Genome Res., 8: 195-202, 1998). The finishing of the sequence was carried out using the GMPTB software package (Frangeul, L., et al., Microbiology, 145: 2625-2634, 1999). The finishing step corresponds to the resequencing of the regions where the sequence is insecure and the sequencing of the regions located between the contigs. It was carried out either by sequencing PCR products or by walking on the clones of the bank. The sequences of the oligonucleotides were defined using the consed and Primo software (Gordon, D., et al., 1998; Li, P., et al., Genomics, 40: 476-485, 1997).

4. Annotation of sequences

The identification of the coding phases (CDS) was carried out using the GMPTB software package. This program combines the results of different methods: (i) identifying open reading phases and sorting them according to their size, (ii) analyzing the probability of being coding using Genemark software (Lukashin, AV, et al., Nucleic Acids Res., 15: 1 107-1 1 15, 1998), (iii) identification of the start of translation (initiation codon and ribosome binding sequence), (iv ) similarity of protein sequence deduced with the protein sequences contained in the sequence banks using the BLASTP software.

The functions of the proteins coded by the identified coding phases were predicted by the analysis of the results of searches for similarities in libraries using the BLASTP software (Altschul, SF, et al., Nucleic Acids Research, 25: 3389-402, 1997).

5. Comparison of the genomes a) Identification of the CDS specific for the strain of L. monocytogenes EGDe and of the strain of L. innocua The set of protein sequences deduced from the predicted coding phases of each genome was compared with the set of sequences protein possibly encoded by the other genome using BLASTP software. A threshold of 75% identity over the entire length of the protein was used to identify the specific proteins of an isolate. This very high value was chosen because it best discriminates the ortholog genes from the paralogs genes (Fitch, W. S., Syst. Zool, 19: 99-1 13, 1970). For protein sequences for which the sequence conservation is high (> 70%) the conservation of the nucleotide sequences of the genes will also be high and could give a signal under conditions of hybridization which are not very stringent. It will be necessary to take this possibility into account when analyzing the test result. b) Identification of the CDS specific for the L. monocytogenes strain serotype 4b with respect to the L. monocytogenes EGDe strain and the L. innocua strain

The chromosomal regions of the strain L. monocytogenes of serotype 4b which are absent from the strains L. monocytogenes EGDe and L. innocua were identified using the Crossmatch / Phrap Package (Phil Green, University of Washington, Seattle, unpublished). This software makes it possible to assemble nucleotide sequences (Phrap) by masking all the sequences or parts of sequence similar to one or more reference sequences (Crossmatch). The reference sequences used were: the complete genome sequence of L. monocytogenes EGD, the complete genome sequence of L. innocua and the sequence of its plasmid. The identification by Crossmatch of the regions which will be masked is based on the search for words of 11 letters identical between the analyzed sequence and the reference sequences and the extension of these words using the software default settings. More information on Crossmatch and Phrap software is available on the website: http://bozeman.mbt.washington.edu/. 6. Examples of annotations

6.1. Genes specific for L. monocytogenes. There is no significant similarity between the nucleotide sequence of the L. monocytogenes gene and the genome of L. innocua.

Table 1

6.2. Specific genes of L. innocua. There is no significant similarity between the nucleotide sequence of the L. innocua gene and the genome of L. monocytogenes.

Table II

6.3. Genes common to the two strains for which the similarity (identity) of the deduced protein sequences is less than 75% and value of the similarity at the nucleotide level.

Table III

6.4. Genes common to L. monocytogenes and L. innocua Table IV

TABLE V: Legends

SEQ ID Nos. 1 - 1 1: nucleotide sequences of 10 Contigs and 1 plasmid from the Listeria innocua assembly. SEQ ID Nos. 12 - 689: nucleotide sequences of specific proteins of L. innocua (absent from Z. monocytogenes-EGD).

SEQ ID Nos. 690 - 1067: nucleotide sequences of the specific proteins of L. monocytogenes-EGD (absent from L. innocua).

SEQ ID Nos. 1068 - 2041: nucleotide sequences of 974 contigs originating from the assembly of Listeria monocytogenes-4b (1,231,537 bases).

SED ID Nos. 2042 - 2056: additional sequences for examples of annotation. TABLE V

TABLE VI: Legends

SEQ ID Nos. 2059-2601: nucleotide sequences of 543 genes specific for Listeria innocua Clipl 1262; with the SEQ ID identifier in the first column, the gene name in the second column, the IPF number in the third column (“Institut Pasteur” identifier number allowing the sequence to be correlated with the sequences in Table V) and in the last column the corresponding annotation.

TABLE VI

SEQID Name IPFID Function

SEQ ID N ° 2059 pliOOOl 4106.2 Unknown, similar to insertion sequence ATP binding protein

SEQIDN ⁰ 2060 pli0002 6602.1 Unknown

SEQIDN ⁰ 2061 pli0003 4103.1 Unknown, similar to transposase

SEQIDN ⁰ 2062 pli0004 4102.1 Unknown, similar to DNA methylase

SEQIDN ⁰ 2063 pli0005 4099.1 Unknown

SEQIDN ⁰ 2064 pliOOOό 4098.1 Unknown

SEQIDN ⁰ 2065 pli0007 4097.1 Unknown

SEQIDN ⁰ 2066 pli0008 4095.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2067 pli0009 4092.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2068 pliOOlO 4088.1 Unknown

SEQ IDN ° 2069 pliOOll 4086.1 Unknown, hypothetical gene

SEQIDN ⁰ 2070 pli0012 4084.1 Transposase

SEQ ID N ° 2071 pliOO 13 4081.1 Unknown, similar to unknown protein

SEQ ID N ° 2072 pliOO 14 4079.1 Unknown, similar to unknown protein

SEQ ID N ° 2073 pliOO 15 4077.1 Unknown, similar to plasmid replication protein

SEQ ID N ° 2074 pliOO 16 6241.1 Unknown, similar to transposase C-terminal part

SEQIDN ⁰ 2075 pli0017 4073.1 Unknown

SEQ IDN ° 2076 pli0018 4195.3 transposase (truncated)

SEQIDN ⁰ 2077 pli0019 4194.3 Transposase, truncated

SEQIDN ⁰ 2078 pli0020 4193.1 Unknown, similar to transposase

SEQIDN ⁰ 2079 pli0021 4192.1 Unknown, similar to putative helicase

SEQ ID N ° 2080 pli0022 4184.1 Unknown, similar to plasmid replication protein B

SEQ ID N ° 2081 pli0023 4183.1 unknown, similar to plasmid replication initiation protein

SEQ ID N ° 2082 pli0024 4182.1 Unknown, similar to transposase

SEQIDN ⁰ 2083 pli0025 4181.1 Unknown, similar to transposase

SEQ ID N ° 2084 pli0026 4179.1 Unknown, similar to gram positive plasmid replication protein B

SEQIDN ⁰ 2085 pli0027 4178.1 Unknown, similar to helicase

SEQIDN ⁰ 2086 pli0028 6113.2 Transposase

SEQIDN ⁰ 2087 pli0029 6111.1 Unknown, similar to DNA methyltransferase

SEQ ID N ° 2088 pli0030 6109.1 Unknown

SEQ ID N ° 2089 pli0031 6606.1 Unknown

SEQ ID N ° 2090 pli0032 6106.2 Transposase

SEQ ID N ⁰ 2091 pli0033 6128.2 Pseudogene, similar to C-tenninal part of arsenite-translocating ATPase

SEQ ID N ° 2092 pli0034 6127.1 Unknown, similar to arsenical resistance operon repressor

SEQ ID N ° 2093 pli0035 6126.1 unknown, similar to arsenical resistance operon trans-acting repressor ArsD

SEQ ID N ° 2094 pli0036 6125.1 Unknown, similar to arsenical resistance operon repressor

SEQ ID N ° 2095 pli0037 6124.1 Unknown, similar to arsenical pump-driving ATPase

SEQ ID N ° 2096 pli0038 6122.1 unknown, similar to possible arsenic resistance membrane transport protein ArsB

SEQ ID N ° 2097 pli0039 6120.1 Unknown, similar to heavy metal membrane efflux protein

SEQ ID N ° 2098 pli0040 61 18.1 Unknown, similar to flavoprotein oxidoreductase

SEQ ID N ° 2099 pli0041 61 15.1 Unknown, similar to ABC transporter ATP-binding protein

SEQ ID N ⁰ 2100 pli0042 6605.1 Similar to transposase, N-terminal part

SEQ ID N ⁰ 2101 pli0043 4177.2 Transposase

SEQ ID N ⁰ 2102 pli0044 4175.1 Unknown, similar to NADH peroxidase

SEQ ID N ⁰ 2103 pli0045 4174.1 Pseudogene, similar to glycine-betaine binding protein (ABC transporter)

SEQ ID N ⁰ 2104 pli0046 4170.1 Unknown, hypothetical protein

SEQ ID N ⁰ 2105 pli0047 4169.1 Unknown

SEQ ID N ⁰ 2106 pli0048 4168.1 Unknown, similar to heavy metal-transporting ATPase

SEQ ID N ⁰ 2107 pli0049 4166.1 Transposase

SEQ ID N ⁰ 2108 pli0050 4164.1 Unknown, similar to the two components sensor protein kdpD

SEQ ID N ⁰ 2109 pli0051 4160.1 Unknown, similar to the two components response regulator KdpE

SEQ ID N ⁰ 21 10 pli0052 4158.1 Unknown, similar to potassium-transporting atpase a chain

SEQ ID N ⁰ 21 1 1 pli0053 4157.1 Unknown, similar to potassium-transporting atpase b chain

SEQ ID N ⁰ 21 12 pli0054 4154.1 Unknown

SEQ ID N ⁰ 21 13 pli0055 4153.1 Unknown, similar to potassium-transporting atpase c chain

SEQ ID N ⁰ 21 14 pli0056 4150.1 Unknown, similar to resolvase / integrase

SEQ ID N ⁰ 21 15 pli0057 4148.1 Unknown, similar to DNA transposition protein

SEQ ID N ⁰ 21 16 pli0058 4147.1 Unknown, similar to transposase

SEQ ID N ⁰ 21 17 pli0059 4146.1 Unknown, similar to invertase

SEQ ID N ⁰ 21 18 pli0060 4145.1 Unknown, similar to cadmium resistance accessory protein

SEQ ID N ⁰ 21 19 pli0062 4140.1 Unknown, similar to unknown protein

SEQ ID N ⁰ 2120 pli0063 4139.1 Unknown, similar to transposase

SEQIDN ⁰ 2121 pli0064 4138.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2122 pli0065 4137.1 Unknown

SEQIDN ⁰ 2123 pli0066 4136.1 Unknown

SEQIDN ⁰ 2124 pli0067 4135.1 Unknown, similar to UV-damage repair protein

SEQIDN ⁰ 2125 pli0068 4132.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2126 pli0069 4130.1 Unknown, similar to plasmid copy control protein repB

SEQIDN ⁰ 2127 pli0070 4128.1 Unknown, similar to plasmid replication protein

SEQIDN ⁰ 2128 pli0071 4124.1 Unknown, similar to transposase

SEQIDN ⁰ 2129 pli0072 4123.1 Unknown, similar to transposase

SEQIDN ⁰ 2130 pli0073 4122.1 unknown

SEQIDN ⁰ 2131 pli0074 4121.1 Unknown

SEQIDN ⁰ 2132 pli0075 4120.1 Unknown

SEQIDN ⁰ 2133 pli0076 4116.1 Transposase

SEQIDN ⁰ 2134 pli0077 4113.1 Unknown, similar to transposase N-terminal part

SEQIDN ⁰ 2135 pli0078 4112.1 Unknown, similar to enolase (phosphopyruvate hydratase), truncated C-terminal end

SEQIDN ⁰ 2136 pli0079 4111.1 Unknown, similar to Na + / H + antiporter

SEQIDN ⁰ 2137 pli0080 4107.2 Unknown

SEQIDN ⁰ 2138 Lin0059 4543.1 Unknown

SEQIDN ⁰ 2139 Lin0060 4535.1 unknown

SEQIDN ⁰ 2140 LinOOόl 4533.1 unknown, hypothetical protein

SEQIDN ⁰ 2141 Lin0062 4532.1 unknown, hypothetical protein

SEQIDN ⁰ 2142 Lin0063 4531.1 Unknown, pseudogene

SEQIDN ⁰ 2143 Lin0064 4522.1 unknown

SEQIDN ⁰ 2144 Lin0065 4521.1 unknown

SEQIDN ⁰ 2145 Lin0066 4520.1 Unknwon

SEQIDN ⁰ 2146 Lin0071 4508.1 Unknown, similar to integrase

SEQIDN ⁰ 2147 Lin0072 4506.1 unknown

SEQIDN ⁰ 2148 Lin0073 4503.2 Unknown, similar to a putative repressor protein [Bacteriophage Al 18]

SEQIDN ⁰ 2149 Lin0074 6149.2 unknown, highly similar to gp37 [Bacteriophage Al 18]

SEQIDN ⁰ 2150 Lin0075 6148.2 unknown, highly similar to gp37-l [Bacteriophage Al 18]

SEQIDN ⁰ 2151 Lin0076 6544.1 Unknown

SEQIDN ⁰ 2152 Lin0077 6317.1 unknown, identical to gp40 [Bacteriophage Al 18]

SEQIDN ⁰ 2153 Lin0079 6145.1 unknown

SEQIDN ⁰ 2154 Lin0080 6144.1 unknown, similar to similar to anti-repressor [Bacteriophage Al 18]

SEQIDN ⁰ 2155 Lin0081 6142.1 unknown, highly similar to gp43 [Bacteriophage Al 18]

SEQIDN ⁰ 2156 Lin0082 6453.2 Unknown

SEQIDN ⁰ 2157 Lin0083 6139.1 unknown, highly similar to gp45 [Bacteriophage Al 18]

SEQIDN ⁰ 2158 Lin0084 6138.3 unknown, highly similar to gp47 [Bacteriophage Al 18]

SEQIDN ⁰ 2159 Lin0085 6136.2 putative recombinase [Bacteriophage Al 18]

SEQIDN ⁰ 2160 Lin0086 3386.1 Unknown, similar to protein gp49

SEQIDN ⁰ 2161 Lin0087 6543.1 Unknown, similar to phage protein

SEQIDN ⁰ 2162 Lin0089 3379.1 unknown, similar to gp51 [Bacteriophage Al 18]

SEQIDN ⁰ 2163 Lin0090 3378.1 unknown,

SEQIDN ⁰ 2164 Lin0091 3375.1 unknown, similar to phage proteins

SEQIDN ⁰ 2165 Lin0092 6542.1 Unknown

SEQIDN ⁰ 2166 Lin0093 3374.1 unknown

SEQIDN ⁰ 2167 Lin0094 3373.1 unknown, highly similar to gp55 [Bacteriophage Al 18]

SEQIDN ⁰ 2168 Lin0095 3371.1 unknown, highly similar to gp59 [Bacteriophage Al 18]

SEQIDN ⁰ 2169 Lin0096 3370.1 unknown

SEQIDN ⁰ 2170 Lin0102 3363.1 unknown

SEQIDN ⁰ 2171 Lin0103 3362.1 unknown

SEQIDN ⁰ 2172 Lin0104 3361.1 unknown, highly similar to putative terminase small subunit [Bacteriophage Al 18]

SEQIDN ⁰ 2173 Lin0109 3354.1 unknown, highly similar to major capsid protein [Bacteriophage Al 18]

SEQIDN ⁰ 2174 LinOllO 6540.1 Protein gp7 [Bacteriophage Al 18]

SEQIDN ⁰ 2175 LinOllό 3345.1 unknown, highly similar to gpl3 [Bacteriophage Al 18] (truncated, C-teπninal end)

SEQIDN ⁰ 2176 Lin0120 3333.1 unknown, highly similar to gpl 7 [Bacteriophage Al 18]

SEQIDN ⁰ 2177 Lin0121 3330.1 unknown, highly similar to gp 18 [Bacteriophage Al 18]

SEQIDN ⁰ 2178 Lin0122 3329.1 unknown, highly similar to gp 19 [Bacteriophage A 118]

SEQIDN ⁰ 2179 Lin0123 3326.1 unknown, similar to gp20 [Bacteriophage Al 18]

SEQIDN ⁰ 2180 Lin0124 3325.1 Unknown

SEQIDN ⁰ 2181 Lin0125 6539.1 Unknown

SEQIDN ⁰ 2182 Lin0128 3320.1 L-alanoyl-D-glutamate peptidase [Bacteriophage A500 from Listeria]

SEQIDN ⁰ 2183 Lin0129 3319.1 Unknown

SEQIDN ⁰ 2184 LinOHO 3291.1 unknown

SEQIDN ⁰ 2185 Lin0141 3290.1 Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQIDN ⁰ 2186 Lin0142 3283.1 unknown

SEQIDN ⁰ 2187 Lin0148 3273.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2188 Lin0154 3255.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2189 Lin0167 3232.1 Unknown

SEQIDN ⁰ 2190 Lin0187 3183.1 Unknwon

SEQIDN ⁰ 2191 Lin0188 3180.1 Unknown

SEQIDN ⁰ 2192 Lin0189 6538.1 Unknown

SEQIDN ⁰ 2193 Lin0197 3160.1 unknown, similar to chloromuconate cycloisomerase ykfB of B. subtilis

SEQIDN ⁰ 2194 Lin0198 3159.1 unknown, P45 related protein

SEQIDN ⁰ 2195 Lin0199 3157.1 unknown, some similarities to probable beta-lactamase

SEQIDN ⁰ 2196 Lin0200 3152.1 Unknown, similar to ABC transporter oligopeptide-binding protein

SEQIDN ⁰ 2197 Lin0201 3148.1 Unknown, similar to dipeptide ABC transporter

SEQIDN ⁰ 2198 Lin0202 3147.1 unknwon, surface anchored protein (LPXTG motif)

SEQIDN ⁰ 2199 Lin0290 1614.1 unknown, intemalin like protein (LPXTG motif)

SEQ ID N ° 2200 Lin0295 1601.1 Unknown, similar to intemalin proteins

SEQIDN ⁰ 2201 Lin0307 1578.1 unknown, similar to ABC transporters (ATP-binding protein)

SEQ ID N ° 2202 Lin0308 1577.1 unknown, similar to hypothetical proteins

SEQ ID N ° 2203 Lin0332 1526.1 unknown, similar to putative permeases

SEQ ID N ° 2204 Lin0338 1513.1 unknown

SEQ ID N ° 2205 Lin0345 1492.1 unknown

SEQ ID N ° 2206 Lin0349 1484.1 Unknown

SEQ ID N ° 2207 Lin0357 1466.1 Unknown

SEQ ID N ° 2208 Lin0372 1442.1 unknown, probable cell surface protein (LPXTG motif)

SEQ ID N ° 2209 Lin0397 1390.1 unknown

SEQIDN ⁰ 2210 Lin0398 1389.1 unknown

SEQIDN ⁰ 2211 Lin0399 1388.1 unknown

SEQIDN ⁰ 2212 Lin0415 1348.1 unknown, probable cell surface protein (LPXTG motif)

SEQIDN ⁰ 2213 Lin0416 1346.1 Unknown, similar to transcription regulator

SEQIDN ⁰ 2214 Lin0417 1345.1 Unknown, similar to unknown protein

SEQIDN ⁰ 2215 Lin0418 1344.1 Unknown, similar to ABC transporter, ATP-binding protein

SEQIDN ⁰ 2216 Lin0419 1342.1 Unknown, similar to ABC transporter, ATP-binding protein

SEQIDN ⁰ 2217 Lin0426 1323.1 Unknown

SEQIDN ⁰ 2218 Lin0453 1260.1 unknown

SEQIDN ⁰ 2219 Lin0454 1259.1 unknown, similar to cell wall-associated protein precursor wapA (B. subtilis)

SEQ ID N ° 2220 Lin0455 1239.1 unknown

SEQ ID ⁰ 2221 Lin0456 1236.1 unknown

SEQ ID N ° 2222 Lin0464 1216.1 Unknown, similar to putative transcription regulator

SEQ ID N ° 2223 Lin0465 1215.1 unknown, conserved hypothetical protein, similar to yoaZ B. subtilis

SEQ ID N ° 2224 Lin0476 1 191.1 unknown

SEQ ID N ° 2225 Lin0477 1 188.1 unknwon

SEQ ID N ° 2226 Lin0478 1 186.1 unknown

SEQ ID N ° 2227 Lin0479 1 183.1 Unknown

SEQ ID N ° 2228 Lin0480 6504.1 Unknown, putative secreted protein

SEQ ID N ° 2229 Lin0481 6525.1 pseudogene

SEQ ID N ° 2230 Lin0486 1 171.1 unknown, similar to unknown proteins

SEQ ID N ⁰ 2231 Lin0510 1 125.1 Unknown

SEQ ID N ° 2232 Lin0521 1096.1 Unknown, similar to HsdR type IC restriction subunit

SEQ ID N ° 2233 Lin0522 1091.1 Unknown, similar to HsdM type IC modification subunit

SEQ ID N ° 2234 Lin0523 1088.1 Unknown, similar to specificity determinant HsdS

SEQ ID N ° 2235 Lin0524 1087.1 Unknown, similar to bacteriophage integrase

SEQ ID N ° 2236 Lin0525 1086.1 Unknown, similar to specificity determinant HsdS

SEQ ID N ° 2237 Lin0553 1021.1 Unknown, similar to intemalin protein

SEQ ID N ° 2238 Lin0554 1018.1 unknown, probable cell surface protein (LPXTG motif)

SEQ ID N ° 2239 Lin0557 1012.1 Unknown

SEQ ID N ° 2240 Lin0558 1010.1 Unknown, similar to intemalin protein

SEQ ID N ⁰ 2241 Lin0559 1007.1 unknown, probable cell surface protein (LPXTG motif)

SEQ ID N ° 2242 Lin0560 1005.1 Unknown

SEQ ID N ° 2243 Lin0561 1004.1 Unknown, similar to unknown protein

SEQ ID N ° 2244 Lin0661 815.1 unknown, intemalin like protein (LPXTG motif)

SEQ ID N ° 2245 Lin0665 805.1 unknown, highly similar to ORFA of Listeria seeligeri, (LPXTG motif)

SEQ ID N ° 2246 Lin0677 781.1 unknown, conserved hypothetical protein

SEQ ID N ° 2247 Lin0678 779.1 Unknown

SEQ ID N ° 2248 Lin0679 776.1 Unknown, similar to unknown protein

SEQ ID N ° 2249 Lin0739 652.1 unknown, intemalin like protein (LPXTG)

SEQ ID N ° 2250 Lin0740 648.1 unknown, probable cell surface protein (LPXTG motif)

SEQ ID N ⁰ 2251 Lin0746 639.1 Unknown, similar to ABC transporter, ATP-binding protein (truncated, N-terminal part)

SEQ ID N ° 2252 Lin0772 589.1 unknown

SEQ ID N ° 2253 Lin0801 529.1 unknown, similar to two-component response regulators

SEQ ID N ° 2254 Lin0802 528.2 unknown, similar to two-component sensor histidine kinases

SEQ ID N ° 2255 Lin0803 526.1 Unknown, surface protein (LPXTG motif)

SEQ ID N ° 2256 Lin0804 523.1 Unknown, similar to unknown protein

SEQ ID N ° 2257 Lin0805 522.1 Unknown, similar to oxidoreductase

SEQ ID N ° 2258 Lin0806 521.1 Unknown, similar to transcriptional regulator, MerR family

SEQ ID N ° 2259 Lin0822 485.1 Unknown, similar to transport protein (Truncated, N-terminal part)

SEQ ID N ° 2260 Lin0823 484.1 Unknown, similar to transport protein (truncated, C-terminal part)

SEQ ID N ⁰ 2261 Lin0827 472.1 unknown, similar to transposase

SEQ ID N ° 2262 Lin0833 457.1 unknown

SEQ ID N ° 2263 Lin0834 455.1 unknown, some similarities to hypothetical proteins

SEQ ID N ° 2264 Lin0835 454.1 unknown

SEQ ID N ° 2265 Lin0842 440.1 Unknown, similar to amidases

SEQ ID N ° 2266 Lin0864 395.1 Unknown, similar to transcription regulator

SEQ ID N ° 2267 Lin0865 394.1 unknown, hypothetical protein

SEQ ID N ° 2268 Lin0866 392.1 unknown, similar to ABC transporters, ATP-binding protein homologue

SEQ ID N ° 2269 Lin0867 391.1 unknown

SEQ ID N ° 2270 Lin0868 390.1 unknown

SEQ ID N ⁰ 2271 Lin0877 372.1 unknown (truncated, N-terminal part)

SEQ ID N ° 2272 Lin0878 371.1 unknown (truncated, C-terminal part)

SEQ ID N ° 2273 Lin0903 325.1 Unknown, truncated N-terminal part

SEQ ID N ° 2274 Lin0904 324.1 Unknown (truncated, C-terminal end)

SEQ ID N ° 2275 Lin0915 302.1 Unknown, similar to phosphotransferase System enzyme IIC (truncated, N-terminal end)

SEQ ID N ° 2276 Lin0916 301.1 Unknown, similar to phosphotransferase System enzyme IIC (truncated, C-terminal end)

SEQ ID N ° 2277 Lin0940 6518.1 unknown, similar to heat shock protein HtpG (truncated, C-terminal part)

SEQ ID N ° 2278 Lin 1056 36.1 unknown

SEQ ID N ° 2279 Lin 1064 20.1 unknown, similar to autolysin (amidase)

SEQ ID N ° 2280 Linl065 17.3 Unknown

SEQ ID N ⁰ 2281 Lin 1066 14.4 unknown, similar to dolichol phosphate mannose synthase

SEQ ID N ° 2282 Lin 1067 13.1 unknown

SEQ ID N ° 2283 Linl068 12.1 unknown, similar to hypothetical protein 3 (capsulation locus) of Haemophilus influenzae

SEQ ID N ° 2284 Lin 1069 9.1 unknown

SEQ ID N ° 2285 Lin 1073 3.1 unknown, similar to galactosamine-containing minor teichoic acid biosynthesis protein GgaA

SEQ ID N ° 2286 Lin 1074 2.2 Unknown, similar to B. subtilis TagF protein (probable CDPglycerol giycerophosphotransferase)

SEQ ID N ° 2287 Linl075 4502.2 unknown, similar to teichoic acid biosynthesis protein B precursor

SEQ ID N ° 2288 Linl082 4486.1 unknown

SEQ ID N ° 2289 Linl083 4485.1 unknown

SEQ ID N ° 2290 Lin 1084 4484.1 Unknown

SEQ ID N ⁰ 2291 Lin 1085 4483.1 unknown

SEQ ID N ° 2292 Linl086 4482.1 unknown

SEQ ID N ° 2293 Lin 1090 4478.1 unknown

SEQ ID N ° 2294 Linen 1091 4477.1 unknown

SEQ ID N ° 2295 Lin 1099 4460.1 unknown

SEQ ID N ° 2296 Lin 1 100 4459.1 unknown

SEQ ID N ° 2297 Linl l77 4309.1 unknown

SEQ ID N ° 2298 Lin 1204 4232.1 unknown, similar to intemalin proteins (LPXTG motif)

SEQ ID N ° 2299 Linl209 4220.1 unknown

SEQ ID N ° 2300 Linl210 4219.1 unknown

SEQ ID N ⁰ 2301 Linl21 1 4218.1 unknown

SEQ ID N ° 2302 Linl212 4217.1 unknown

SEQ ID N ° 2303 Lin 1220 6513.1 Unknown

SEQ ID N ° 2304 Linl231 6002.1 Unknown, similar to site-specific recombinase for integration and excision [bacteriophage phi- 105]

SEQ ID N ° 2305 Linl232 6003.1 Unknown, similar to unknown protein

SEQ ID N ° 2306 Linl233 6005.1 Unknown, similar to bacteriophage phi- 105 ORF2 protein

SEQ ID N ° 2307 Linl234 6006.4 Unknown, similar to immunity repressor [bacteriophage phi- 105]

SEQ ID N ° 2308 Linl235 6007.4 Unknown, similar to transcription regulator

SEQ ID N ° 2309 Linl236 6189.4 Unknown

SEQ ID N ⁰ 2310 Linl237 6512.2 Unknown

SEQ ID N ° 231 1 Linl238 651 1.1 Unknown

SEQ ID N ⁰ 2312 Linl239 6188.1 Unknown

SEQ ID N ⁰ 2313 Lin 1240 6186.1 Unknown

SEQ ID N ⁰ 2314 Lin 1241 6185.5 Unknown, similar to bacteriophage protein

SEQ ID N ⁰ 2315 Lin 1242 6658.1 unknown, some similarities to phage related proteins

SEQ ID N ⁰ 2316 Lin 1243 6659.1 unknown, similar to hypothetical protein 44 - Staphylococcus aureus phage phi PVL

SEQ ID N ⁰ 2317 Lin 1244 6660.1 unknown

SEQ ID N ⁰ 2318 Lin 1245 6661.1 unknown, similarities Staphylococcus aureus prophage phiPV83

SEQ ID N ⁰ 2319 Lin 1246 6662.1 unknown

SEQ ID N ° 2320 Lin 1247 6663.1 unknown

SEQ ID N ⁰ 2321 Lin 1248 6664.1 unknown, similar to hypothetical protein, Staphylococcus aureus phage phi PVL

SEQ ID N ° 2322 Lin 1249 6665.1 unknown

SEQ ID N ° 2323 Linl250 6666.1 unknown

SEQ ID N ° 2324 Linl251 6667.1 Unknown

SEQ ID N ° 2325 Linl252 6668.1 Unknown

SEQ ID N ° 2326 Linl253 6669.1 Unknown

SEQ ID N ° 2327 Linl254 6670.1 unknown, similar to phage intagrase proteins

SEQ ID N ° 2328 Linl255 6671.1 unknown

SEQ ID N ° 2329 Linl256 6040.3 Unknown

SEQ ID N ° 2330 Linl257 2999.2 Unknown

SEQ ID N ⁰ 2331 Linl258 2998.1 Unknown

SEQ ID N ° 2332 Linl259 2997.1 Unknown, similar to protein gp66 [Bacteriophage Al 18]

SEQ ID N ° 2333 Lin 1260 6202.2 unknown, similar to probable antirepressor - Bacillus subtilis phage SPBc2

SEQ ID N ° 2334 Lin 1261 561 1.4 Unknown

SEQ ID N ⁰ 2335 Lin 1262 5609.3 Unknown

SEQ ID N ° 2336 Lin 1263 6588.1 Unknown

SEQ ID N ° 2337 Lin 1264 5606.1 Unknown

SEQ ID N ° 2338 Lin 1265 5605.1 Unknown

SEQ ID N ° 2339 Linl266 5604.1 Unknown, similar to phage protein

SEQ ID N ° 2340 Linl267 5602.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ⁰ 2341 Lin 1268 5600.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2342 Lin 1269 5597.1 Unknown, similar to unknown protein

SEQ ID N ° 2343 Lin 1270 5593.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2344 Linl271 5590.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2345 Lin 1272 6394.1 unknown

SEQ ID N ° 2346 Linl273 5587.1 Unknown, similar to unknown protein

SEQ ID N ° 2347 Lin 1274 5586.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2348 Linl275 5584.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2349 Lin 1276 5583.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2350 Lin 1277 5580.1 unknown

SEQ ID N ⁰ 2351 Linl278 5579.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ⁰ 2352 Linl279 5577.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2353 Linl280 5576.1 Unknown, similar to a B. subtilis PBSX phage protein

SEQ ID N ° 2354 Lin 1281 6636.1 Unknown, similar to phage protein (truncated, C-terminal end)

SEQ ID N ° 2355 Linl282 5575.1 Unknown, similar to phage proteins

SEQ ID N ° 2356 Linl283 5569.1 Unknown, similar to a B. subtilis PBSX prophage protein

SEQ ID No. 2357 Linl284 5568.1 Unknown, similar to a B. subtilis PBSX prophage protein

SEQ ID No. 2358 Linl285 5567.1 Unknown, similar to a B. subtilis PBSX prophage protein

SEQ ID No. 2359 Linl286 5565.1 Unknown, similar to a B. subtilis PBSX prophage protein

SEQ ID N ° 2360 Linl287 5564.1 Unknown, similar to a B. subtilis PBSX prophage protein

SEQ ID N ⁰ 2361 Linl288 5561.1 Unknown, similar to a B. subtilis PBSX prophage protein

SEQ ID N ° 2362 Lin 1289 5560.1 Unknown, similar to unknown protein

SEQ ID N ° 2363 Lin 1290 5558.1 Unknown

SEQ ID N ° 2364 Linl291 5556.1 Unknown

SEQ ID N ° 2365 Lin 1292 6589.1 Unknown

SEQ ID N ° 2366 Linl293 5555.1 Unknown

SEQ ID N ° 2367 Linl294 5553.1 Unknown

SEQ ID N ° 2368 Lin 1295 5551.1 Unknown, similar to holin

SEQ ID N ° 2369 Lin 1296 5550.1 unknown, similar to hypothetical protein - phage SPP 1

SEQ ID N ° 2370 Lin 1297 6590.1 Unknown, similar to Portein gp28 [Bacteriophage Al 18]

SEQ ID N ⁰ 2371 Lin 1298 5546.1 unknown

SEQ ID N ⁰ 2372 Lin 1299 5545.1 unknown

SEQ ID N ° 2373 Lin 1300 5543.1 unknown

SEQ ID N ° 2374 Linl301 5541.1 Unknown

SEQ ID N ° 2375 Lin 1302 6591.1 Unknown

SEQ ID N ° 2376 Linl328 5484.1 unknown, intemalin like protein (LPXTG motif)

SEQ ID N ⁰ 2377 Linl378 6432.2 Unknown, weakly similar to B. subtilis comG operon protein 7 (comGG)

SEQ ID N ° 2378 Linl379 2316.2 Unknown, similar to B. subtilis comG operon protein 6

SEQ ID N ° 2379 Linl381 2318.1 Unknown, similar to comG operon protein 4 (comGD)

SEQ ID N ° 2380 Lin 1450 2451.1 Unknown

SEQ ID N ⁰ 2381 Linl451 2455.1 Unknwon

SEQ ID N ° 2382 Linl452 2456.1 unknown

SEQ ID N ⁰ 2383 Linl618 2777.1 Unknown, similar to a protein encoded by Th916

SEQ ID N ° 2384 Linl619 2778.1 Unknown

SEQ ID N ° 2385 Lin 1620 2779.1 Unknown, similar to putative iron-sulfur flavoprotein

SEQ ID N ⁰ 2386 Lin 1621 2781.1 unknown, similar to ketoacyl reductases

SEQ ID N ° 2387 Lin 1622 2783.1 Unknown, similar to transcriptional regulator (MerR family)

SEQ ID N ° 2388 Linl623 2785.1 Unknown, similar to site-specific recombinase tnpX - Clostridium perfringens transposon Tn4451 (N terminal part)

SEQ ID N ° 2389 Lin 1624 2788.1 unknown, similar to site-specific recombinase tnpX - Clostridium perfringens transposon Tn4451

SEQ ID N ° 2390 Linl695 2923.1 Unknown, similar to unknown protein

SEQ ID N ⁰ 2391 Lin 1696 2924.1 unknown

SEQ ID N ° 2392 Lin 1700 2934.1 Unknown, similar to N-acetylmuramoyl-L-alanine amidase (N-terminal part) and to L-alanoyl-D- glutamate peptidase (C-terminal part)

SEQ ID N ° 2393 Lin 1701 6597.1 Unknown

SEQ ID N ° 2394 Lin 1702 6598.1 Unknown similar to holin from bacteriophage

SEQ ID N ° 2395 Linl703 2937.1 unknown

SEQ ID N ° 2396 Lin 1704 2939.1 Unknown

SEQ ID N ° 2397 Lin 1705 2940.1 Unknown

SEQ ID N ° 2398 Lin 1706 6599.1 Unknown, similar to protein gp22 [Bacteriophage Al 18]

SEQ ID N ° 2399 Linl707 2941.1 Unknown

SEQ ID N ° 2400 Linl708 2942.1 Unknown

SEQ ID N ⁰ 2401 Lin 1709 2943.1 Unknown

SEQ ID N ° 2402 Linl710 2944.2 Unknown, similar to unknown protein

SEQ ID N ° 2403 Linl71 1 2945.2 Unknown

SEQ ID N ° 2404 Linl712 2946.2 Unknown

SEQ ID N ° 2405 Linl713 2947.1 Unknown

SEQ ID N ° 2406 Linl 714 2949.1 Unknown

SEQ ID N ° 2407 Linl715 2950.1 Unknown

SEQ ID N ° 2408 Linl716 2958.1 Unknown, similar to minor capsid protein 1608 - Lactobacillus phage phi-gle

SEQ ID N ° 2409 Linl717 2959.1 Unknown

SEQ ID N ⁰ 2410 Linl718 2961.1 unknown

SEQ ID N ⁰ 241 1 Linl 719 2964.1 unknown

SEQ ID N ⁰ 2412 Lin 1720 2966.1 unknown, weakly similar to hypothetical protein of bacteriophage Félix 01

SEQ ID N ⁰ 2413 Lin 1721 2967.1 unknown

SEQ ID N ⁰ 2414 Lin 1722 2968.1 unknown

SEQ ID N ⁰ 2415 Lin 1723 2969.1 unknown

SEQ ID N ⁰ 2416 Lin 1724 2970.1 unknown

SEQ ID N ⁰ 2417 Lin 1725 2973.1 unknown

SEQ ID N ⁰ 2418 Lin 1726 2975.1 unknown, similar to hypothetical proteins

SEQ ID N ⁰ 2419 Lin 1727 2976.1 unknown

SEQ ID N ° 2420 Lin 1728 2978.1 unknown, similar to hypothetical proteins

SEQ ID N ° 2421 Lin 1729 6600.1 Unknown

SEQ ID N ° 2422 Linl730 2981.1 unknown, some similarities to plasmid-related proteins

SEQ ID N ° 2423 Lin 1731 2982.1 unknown, some similarities to conserved hypothetical proteins

SEQ ID N ° 2424 Linl732 2985.1 unknown, some similarities to phage related proteins

SEQ ID N ° 2425 Linl 733 2987.1 unknown, weakly similar to phage related proteins

SEQ ID N ° 2426 Lin 1734 2988.1 unknown

SEQ ID N ° 2427 Lin 1735 2989.1 Unknown

SEQ ID N ° 2428 Linl736 6601.1 Unknown

SEQ ID N ° 2429 Linl737 2993.1 unknown, weakly similar to methyltransferases

SEQ ID N ° 2430 Linl 738 2995.3 Unknown, similar to a putative antirepressor [Bacteriophage SPBc2]

SEQ ID N ° 2431 Linl 739 6045.4 unknown, similar to protein gp66 of Bacteriophage Al 18

SEQ ID N ° 2432 Lin 1740 6043.1 unknown, similar to hypothetical protein of Lactobacillus phage phi-gle

SEQ ID N ° 2433 Lin 1741 6042.1 unknown, similar to hypothetical protein from phage P2

SEQ ID N ° 2434 Lin 1742 6038.1 unknown

SEQ ID N ° 2435 Lin 1743 6037.1 unknown, similar to phage intagrase proteins

SEQ ID N ° 2436 Lin 1744 6035.1 unknown

SEQ ID N ° 2437 Lin 1745 6032.1 unknown

SEQ ID N ° 2438 Lin 1746 6574.1 Unknown

SEQ ID N ° 2439 Lin 1747 6030.1 unknown

SEQ ID N ° 2440 Lin 1748 6029.1 unknown

SEQ ID N ⁰ 2441 Lin 1749 6028.1 unknown, similar to hypothetical protein, Staphylococcus aureus phage phi PVL

SEQ ID N ° 2442 Linl750 6027.1 unknown

SEQ ID N ° 2443 Linl 751 6026.1 unknown

SEQ ID N ° 2444 Linl 752 6025.1 unknown, similarities Staphylococcus aureus prophage phiPV83

SEQ ID N ° 2445 Lin 1753 6021.1 unknown

SEQ ID N ° 2446 Lin 1754 6019.1 unknown, similar to hypothetical protein 44 - Staphylococcus aureus phage phi PVL

SEQ ID N ° 2447 Linl755 6018.1 unknown, some similarities to phage related proteins

SEQ ID N ° 2448 Linl756 6016.1 unknown, similar to hypothetical protein of Staphylococcus aureus phage phi PVL

SEQ ID N ° 2449 Linl 757 6015.1 unknown

SEQ ID N ° 2450 Linl758 6575.1 Unknown

SEQ ID N ⁰ 2451 Lin 1759 6012.1 unknown

SEQ ID N ° 2452 Lin 1760 6009.4 Unknown, similar to protein gp43 [Bacteriophage Al 18]

SEQ ID N ° 2453 Linl761 6291.1 Unknown, similar to transcription regulator

SEQ ID N ° 2454 Lin 1762 5937.2 Unknown, similar to immunity repressor protein - Bacillus phage phi- 105

SEQ ID N ° 2455 Linl763 5935.2 Unknown, similar to ORF2 [bacteriophage phi- 105]

SEQ ID N ° 2456 Lin 1764 5934.1 Unknown, Listeria prophage protein

SEQ ID N ° 2457 Lin 1765 5933.1 Unknown, similar to integrase

SEQ ID N ° 2458 Lin 1768 5926.1 unknown, similar to Antigen C

SEQ ID N ° 2459 Linlδl l 5313.1 unknown, similar to unknown proteins

SEQ ID N ° 2460 Linl812 5315.1 Unknown, similar to excinuclease ABC (subunit A) (truncated, C-terminal end)

SEQ ID N ⁰ 2461 Linl 813 5317.1 Unknown, similar to excinuclease ABC subunit A

SEQ ID N ° 2462 Linl 814 5319.1 unknown, similar to putative AraC-type regulators

SEQ ID N ° 2463 Linl 898 4696.1 Unknown, similar to putative NAD (P) H oxidoreductase

SEQ ID N ° 2464 Lin 1899 4697.1 Unknown, similar to unknown protein

SEQ ID N ° 2465 Lin 1955 4817.1 unknown

SEQ ID N ° 2466 Lin2001 4896.2 Unknown, hypothetical CDS

SEQ ID N ° 2467 Lin2100 5275.3 unknown, similar to p60-related proteins

SEQ ID N ° 2468 Lin2210 6700.1 Unknown

SEQ ID N ° 2469 Lin2281 3752.1 unknown, probable cell surface protein (LPXTG motif)

SEQ ID N ° 2470 Lin2344 5982.1 Unknown, similar to O6-methylguanine-DNA methyltransferase

SEQ ID N ⁰ 2471 Lin2371 4069.2 Unknown, similar to competence transcription factor ComK, N terminal part

SEQ ID N ° 2472 Lin2373 4899.3 Unknown, similar to AbiD phage protein

SEQ ID N ° 2473 Lin2374 4066.1 Unknown, similar to N-acetylmuramoyl-L-alanine amidase (N-terminal part) and to L-alanoyl-D- glutamate peptidase (C-terminal part)

SEQ ID N ° 2474 Lin2375 4065.1 unknown, similar to phage related proteins

SEQ ID N ° 2475 Lin2376 4062.1 Unknown

SEQ ID N ° 2476 Lin2377 6565.1 Unknown

SEQ ID N ° 2477 Lin2378 6199.6 Unknown

SEQ ID N ° 2478 Lin2379 6046.2 Unknown, similar to protein gp20 [Bacteriophage Al 18]

SEQ ID N ° 2479 Lin2380 6050.1 Unknown, similar to protein gpl9 [Bacteriophage Al 18]

SEQ ID N ° 2480 Lin2381 6051.1 Unknown, similar to protein R372 - Lactobacillus phage phi-gle

SEQ ID N ⁰ 2481 Lin2382 6052.1 Unknown, similar to gpl7 [Bacteriophage Al 18]

SEQ ID N ° 2482 Lin2383 6059.1 unknown, similar to hypothetical protein [Lactobacillus casei bacteriophage A2]

SEQ ID N ° 2483 Lin2384 6063.1 Unknown

SEQ ID N ° 2484 Lin2385 6066.1 Unknown, similar to protein gpl3 [Bacteriophage Al 18]

SEQ ID N ° 2485 Lin2386 6068.1 Unknown

SEQ ID N ° 2486 Lin2387 6070.1 Unknown

SEQ ID N ° 2487 Lin2388 6071.1 Unknown

SEQ ID N ° 2488 Lin2389 6572.1 Unknown

SEQ ID N ° 2489 Lin2390 6075.1 Unknown, similar to main capsid protein Gp34 - Lactobacillus delbrueckii subsp. bulgaricus phage mv4

SEQ ID N ° 2490 Lin2391 6076.1 Unknown

SEQ ID N ⁰ 2491 Lin2392 6078.1 Unknown, similar to protein gp4 [Bacteriophage Al 18]

SEQ ID N ° 2492 Lin2393 6081.1 unknown

SEQ ID N ° 2493 Lin2394 6084.1 unknown

SEQ ID N ° 2494 Lin2395 6085.1 unknown, some similarities to phage-related terminase small subunit homolog yqaS

SEQ ID N ° 2495 Lin2396 6086.1 Unknown

SEQ ID N ° 2496 Lin2397 6087.1 unknown, similar to sigma factor-like positive control protein of B. subtilis

SEQ ID N ° 2497 Lin2398 6570.1 Unknown, hypothetical gene

SEQ ID N ° 2498 Lin2399 6090.1 unknown

SEQ ID N ° 2499 Lin2400 6092.1 Unknown, similar to Lactococcus lactis prophage pi3 protein 45

SEQ ID N ° 2500 Lin2401 6569.1 Unknown

SEQ ID N ⁰ 2501 Lin2402 6093.1 unknown, similar to single-stranded DNA-binding protein

SEQ ID N ° 2502 Lin2403 6096.1 unknown

SEQ ID N ° 2503 Lin2404 6099.1 unknown

SEQ ID N ° 2504 Lin2405 6101.1 unknown

SEQ ID N ° 2505 Lin2406 6502.1 Protein gp52 [Bacteriophage Al 18]

SEQ ID N ° 2506 Lin2407 6104.2 pseudogene

SEQ ID N ° 2507 Lin2408 6698.1 Unknown

SEQ ID N ° 2508 Lin2409 3995.1 unknown, similar to intrgase proteins

SEQ ID N ° 2509 Lin2410 6131.2 unknown, similar to phage related proteins

SEQ ID N ⁰ 2510 Lin241 1 6132.1 unknown

SEQ ID N ⁰ 251 1 Lin2412 6135.1 unknown, highly similar to gp49 [Bacteriophage Al 18]

SEQ ID N ⁰ 2512 Lin2413 3387.3 unknown, highly similar to putative recombinase [Bacteriophage Al 18]

SEQ ID N ⁰ 2513 Lin2414 5720.2 gp47 [Bacteriophage Al 18]

SEQ ID N ⁰ 2514 Lin2415 6697.1 Unknown

SEQ ID N ⁰ 2515 Lin2418 5715.1 Unknown, similar to anti-repressor

SEQ ID N ⁰ 2516 Lin2419 6620.2 Unknown

SEQ ID N ⁰ 2517 Lin2420 5710.1 Unknwon, similar to Bacteriophage A 1 18 protein gp40

SEQ ID N ⁰ 2518 Lin2421 5709.1 Unknwon

SEQ ID N ⁰ 2519 Lin2422 5708.1 Unknown, similar to Bacteriophage Al 18 putative repressor protein

SEQ ID N ° 2520 Lin2423 5706.1 Unknown, similar to Bacteriophage Al 18 protein gp34

SEQ ID N ⁰ 2521 Lin2425 5704.1 unknown

SEQ ID N ° 2522 Lin2454 5645.1 Unknown, similar to 6-phospho-beta-glucosidase

SEQ ID N ° 2523 Lin2455 5644.1 Unknown, similar to transcription antiterminator BglG family

SEQ ID N ° 2524 Lin2456 5643.1 Unknown, similar to unknown proteins

SEQ ID N ° 2525 Lin2457 5642.1 Unknown, similar to PTS System, cellobiose-specific enzyme IIA component

SEQ ID N ° 2526 Lin2458 5640.1 Unknown, similar to PTS System, cellobiose-specific enzyme IIB component

SEQ ID N ° 2527 Lin2459 5638.1 Unknown, similar to PTS System, cellobiose-specific enzyme IIC component

SEQ ID N ° 2528 Lin2487 5052.1 Unknown, similar to unknown proteins

SEQ ID N ° 2529 Lin2494 5039.1 unknown, hypothetical protein

SEQ ID N ° 2530 Lin2537 4955.1 Unknown, similar to intemalin proteins

SEQ ID N ⁰ 2531 Lin2561 6688.1 Unknown, similar to unknown protein

SEQ ID N ° 2532 Lin2562 4067.2 Unknown, similar to unknown protein

SEQ ID N ° 2533 Lin2563 6704.1 Unknown, similar to N-acetylmuramoyl-L-alanine amidase (N-terminal part) and to L-alanoyl-D- glutamate peptidase (C-terminal part)

SEQ ID N ° 2534 Lin2564 4061.3 Unknown

SEQ ID N ° 2535 Lin2565 4060.1 Unknown, similar to protein gp20 [Bacteriophage Al 18]

SEQ ID N ° 2536 Lin2566 4057.1 Unknown, similar to endopeptidase [bacteriophage ML285]

SEQ ID N ° 2537 Lin2567 4054.1 Unknown, similar to Orf53 [bacteriophage bIL285]

SEQ ID N ° 2538 Lin2568 4052.1 Unknown, similar to tail protein [bacteriophage bIL285]

SEQ ID N ° 2539 Lin2569 6564.1 Unknown, similar to Lactococcus lactis prophage pi2 protein 41

SEQ ID N ° 2540 Lin2570 4047.1 Unknown, similar to Orf51 [bacteriophage ML285]

SEQ ID N ⁰ 2541 Lin2571 4046.1 Unknown, similar to Orf50 [bacteriophage bIL285]

SEQ ID N ° 2542 Lin2572 4044.1 Unknown, similar to Orf49 [bacteriophage bIL285]

SEQ ID N ° 2543 Lin2573 4042.1 Unknown, similar to Orf48 [bacteriophage bIL285]

SEQ ID N ° 2544 Lin2574 4041.1 Unknown, similar to Orf47 [bacteriophage ML285]

SEQ ID N ° 2545 Lin2575 4040.1 Unknown, similar to Orf46 [bacteriophage bIL285]

SEQ ID N ° 2546 Lin2576 4039.1 Unknown, similar to capsid protein [bacteriophage bIL285]

SEQ ID N ° 2547 Lin2577 4037.1 Unknown, similar to protease [bacteriophage bIL285]

SEQ ID N ° 2548 Lin2578 4036.1 Unknown, similar to portai protein [bacteriophage bIL285]

SEQ ID N ° 2549 Lin2579 4033.1 Unknown, similar to terminase [bacteriophage bIL285]

SEQ ID N ° 2550 Lin2580 4030.1 Unknown, similar to bacteriophage protein

SEQ ID N ⁰ 2551 Lin2581 4028.1 Unknown, similar to bacteriophage protein

SEQ ID N ° 2552 Lin2582 4026.1 Unknown

SEQ ID N ° 2553 Lin2583 4023.1 Unknown

SEQ ID N ° 2554 Lin2584 4022.1 Unknown

SEQ ID N ° 2555 Lin2585 4021.1 Unknown

SEQ ID N ° 2556 Lin2586 4020.1 Unknown, similar to bacteriophage protein

SEQ ID N ° 2557 Lin2587 4019.1 Unknown, similar to bacteriophage protein

SEQ ID N ° 2558 Lin2588 4016.1 Unknown, similar to bacteriophage protein

SEQ ID N ° 2559 Lin2589 4015.1 Unknown, similar to DEAH-family helicase

SEQ ID N ° 2560 Lin2590 4013.1 Unknown, similar to bacteriophage protein

SEQ ID N ⁰ 2561 Lin2591 4012.1 Unknown, similar to bacteriophage protein

SEQ ID N ° 2562 Lin2592 6696.1 Unknown

SEQ ID N ° 2563 Lin2593 4008.1 Hypothetical gene

SEQ ID N ° 2564 Lin2594 4006.1 Unknown

SEQ ID N ° 2565 Lin2595 4005.1 Unknown

SEQ ID N ° 2566 Lin2596 4003.1 Unknown

SEQ ID N ° 2567 Lin2597 4001.1 Unknown

SEQ ID N ° 2568 Lin2598 6559.1 Unknown

SEQ ID N ° 2569 Lin2599 3998.1 Unknown

SEQ ID N ° 2570 Lin2600 3996.2 unknown

SEQ ID N ⁰ 2571 Lin2601 6130.2 Unknown, similar to bacteriophage integrase

SEQ ID N ° 2572 Lin2602 3994.2 Unknown, similar to phage protein

SEQ ID N ° 2573 Lin2603 3993.1 unknown

SEQ ID N ° 2574 Lin2604 3990.1 unknown

SEQ ID N ° 2575 Lin2605 6557.1 Unknown

SEQ ID N ° 2576 Lin2606 6556.1 Unknown

SEQ ID N ⁰ 2577 Lin2607 3989.1 Unknown, similar to a putative repressor protein [Bacteriophage Al 18]

SEQ ID N ° 2578 Lin2608 3988.1 Unknwon, similar to protein gp35 from Bacteriophage Al 18

SEQ ID N ° 2579 Lin2609 3987.1 Unknown

SEQ ID N ° 2580 Lin2610 3985.1 unknown, similar to integrases

SEQ ID N ⁰ 2581 Lin2656 3889.1 unknown, similar to late competence protein comFC

SEQ ID N ° 2582 Lin2693 3815.1 Unknown

SEQ ID N ° 2583 Lin2703 5735.1 autolysin, amidase

SEQ ID N ° 2584 Lin2723 5767.1 Unknwon, conserved hypothetical protein

SEQ ID N ⁰ 2585 Lin2724 5773.1 unknown, internalin-like protein (LPXTG motif)

SEQ ID N ° 2586 Lin2735 6183.3 Unknown

SEQ ID N ° 2587 Lin2736 6181.1 Unknown

SEQ ID N ° 2588 Lin2741 1716.1 unknown

SEQ ID N ° 2589 Lin2742 1717.1 Unknown

SEQ ID N ° 2590 Lin2743 1718.1 unknown

SEQ ID N ⁰ 2591 Lin2744 1720.1 unknown, similar to hypothetical proteins

SEQ ID N ° 2592 Lin2824 6550.1 Unknown, similar to hydrolase (esterase) (truncated, C-terminal end)

SEQ ID N ° 2593 Lin2825 1892.1 Unknown, similar to hydrolase (esterase) (truncated, N-terminal end)

SEQ ID N ° 2594 Lin2839 1935.1 unknown

SEQ ID N ° 2595 Lin2918 2102.1 unknown

SEQ ID N ° 2596 Lin2921 6547.1 Unknown, similar to unknown protein

SEQ ID N ° 2597 Lin2940 2142.1 unknown, similar to abortive phage resistance mechanism [Lactococcus lactis]

SEQ ID N ° 2598 Lin2941 2143.1 unknown

SEQ ID N ° 2599 Lin2945 2151.1 unknown

SEQ ID N ° 2600 Lin2958 2180.1 Unknown, similar to efflux proteins (truncated, N-terminal end)

SEQ ID N ⁰ 2601 Lin2959 2182.1 Unknown, similar to efflux proteins (truncated, N-terminal end)

TABLE VII: Legends

SEQ ID Nos. 2602 - 2871: nucleotide sequences of the 270 genes specific for Listeria monocytogenes EGDe; with the SEQ ID identifier in the first column, the gene name in the second column, the IPF number in the third column (“Institut Pasteur” identifier number allowing the sequence to be correlated with the sequences in Table V) and in the last column the corresponding annotation.

-

TABLE VII SEQID Name IPFID Function

SEQID No. 2602 lmo0017 587.1 Unknown, similar to Bacillus anthracis CapA protein (polyglutamate capsule biosynthesis) SEQID No. 2603 lmo0036 611.1 Unknown, similar to omithine carbamoyltransferase SEQID No. 2604 lmo0037 613.1 Unknown, similar to amino acid transporter SEQID No. 2605 lmo00 Unknown, conserved hypothetical protein SEQID N ° 2606 lmo0039 615.1 Unknown, similar to carbamate kinase SEQID N ° 2607 lmo0040 616.1 Unknown, conserved hypothetical protein SEQID N ° 2608 lmo0041 617.1 Unknown, conserved hypothetical protein, hypothetical regulator SEQID N ° 2609 lmo0066 2161.2 Unknwon, similar to toxin components SEQID N ° 2610 lmo0067 395.2 Unknown, similar to dinitrogenase reductase ADP-ribosylation system SEQID N ° 2611 lmo0069 392.3 unknown SEQID N ° 2612 lmo0070 390.3 unknown SEQID N ° 2613 lmo0071 389.1 unknown SEQID N ° 2614 lmo0072 4251.1 Unknown SEQID N ° 2615 lmo0073 388.1 unknown SEQID N ° 2616 lmo0074 387.1 Unknown SEQID N ° 2617 lmo0079 380.1 Unknwon SEQI DN ° 2618 lmo0080 379.1 Unknwon SEQID N ° 2619 lmo0081 378.1 Unknwon SEQID N ° 2620 lmo0082 377.1 Unknwon SEQID N ° 2621 lmo0083 376.1 Unknown, similar to transcription regulator (merR family) SEQID N ° 2622 lmo0084 375.1 Unknwon SE 2623 lmo0106 345.1 Unknown, similar to transcription regulator SEQID N ° 2624 lmoOHO 338.1 Unknown, similar to lipase SEQID N ° 2625 lmo0140 2401.1 Unknwon SEQID N ° 2626 lmo0141 3995.1 unknown SEQID N ° 2627 lmo0142 2400.1 unknown SEQID N ° 2398 lmo0 ° 2629 lmo0144 2397.1 Unknown SEQID N ° 2630 lmo0145 4247.1 Unknwon, hypothetical protein SEQID N ° 2631 lmo0146 4246.1 Unknwon, hypothetical protein

SEQIDN ⁰ 2632 lmo0147 2395.1 Unknwon

SEQIDN ⁰ 2633 lmo0148 2394.1 unknown

SEQIDN ⁰ 2634 lmo0149 2393.1

SEQIDN ⁰ 2635 lmo0150 2392.1 unknwon

SEQIDN ⁰ 2636 lmo0151 2391.1 Unknwon

SEQ ID N ° 2637 lmo0171 973.1 Unknwon, similar to intemalin proteins, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2638 ImoOl 72 970.1 Unknown, similar to transposase C-terminal part

SEQ ID N ° 2639 lmo0173 969.1 Unknown, similar to transposase (N-terminal part)

SEQIDN ⁰ 2640 lmo0174 968.1 Unknown, similar to transposase

SEQIDN ⁰ 2641 prfA 1447.1 listeriolysin positive regulatory protein

SEQ ID N ° 2642 plcA 1446.1 phosphatidylinositol-specific phospholipase c

SEQIDN ⁰ 2643 hly 1445.1 listeriolysin O precursor

SEQ ID N ° 2644 mpl 1444.1 Zinc metalloproteinase precursor

SEQ ID N ° 2645 actA 1442.1 actin-assembly inducing protein precursor

SEQIDN ⁰ 2646 plcB 1439.1 phospholipase C

SEQIDN ⁰ 2647 lmo0206 1438.1 Unknwon

SEQ ID N ° 2648 lmo0252 3976.4 Unknown, similar to repressor (penicilinase repressor)

SEQ ID N ° 2649 lmo0253 4262.2 Unknown, similar to penicillinase antirepressor

SEQIDN ⁰ 2650 lmo0254 1859.2 Unknown

SEQIDN ⁰ 2651 lmo0255 1858.1 Unknown, similar to unknown protein

SEQ ID N ° 2652 lmo0257 1856.2 Unknown, similar to unknown protein

SEQIDN ⁰ 2653 inlG 1842.1 intemalin G

SEQIDN ⁰ 2654 inlH 1840.1 intemalin H

SEQIDN ⁰ 2655 inlE 1838.1 intemalin E

SEQIDN ⁰ 2656 lmo0304 2474.1 Unknown

SEQIDN ⁰ 2657 lmo0310 3811.3 Unknown

SEQIDN ⁰ 2658 lmo0311 2336.3 Unknown

SEQ ID N ° 2659 lmo0312 2335.2 Unknown, similar to unknown proteins

SEQIDN ⁰ 2660 lmo0313 2334.1 Unknown, conserved hypothetical protein

SEQ ID N ° 2661 lmo0320 2323.1 Unknown, similar to surface protein (peptidoglycan bound, LPXTG motif)

SEQ ID N ° 2662 lmo0329 3934.1 Unknown, similar to transposase

SEQ ID N ° 2663 lmo0330 3750.2 Unknown, similar to transposase

SEQIDN ⁰ 2664 lmo0332 3754.2 Unknown

SEQ ID N ° 2665 lmo0333 2137.2 Unknown, similar to intemalin proteins, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2666 lmo0334 2138.1 unknown

SEQ ID N ° 2667 lmo0337 2141.1 Unknown

SEQ ID N ° 2668 lmo0338 2142.1 Unknown

SEQ ID N ° 2669 lmo0340 4097.1 Unknown

SEQ ID N ° 2670 lmo0378 2050.1 Unknown

SEQ ID N ⁰ 2671 lmo0379 2049.3 Unknown

SEQ ID N ° 2672 lmo0380 1 1 15.3 Unknown

SEQ ID N ° 2673 lmo0381 1 1 14.1 Unknown

SEQ ID N ° 2674 lmo0409 1074.1 Unknown, similar to intemalin, peptidoglycan bound protein (LPxTG motif)

SEQ ID N ° 2675 lmo0419 1572.1 Unknown, similar to unknown protein

SEQ ID N ° 2676 inlA 1549.1 Intemalin A

SEQ ID N ° 2677 inlB 1547.1 Intemalin B

SEQ ID N ° 2678 lmo0438 1538.1 unknown

SEQ ID N ° 2679 lmo0440 1625.2 Unknown

SEQ ID N ° 2680 lmo0444 1631.1 Unknown, conserved hypothetical protein

SEQ ID N ⁰ 2681 lmo0445 1632.1 Unknown, similar to transcription regulator

SEQ ID N ° 2682 lmo0446 1634.1 Unknown, similar to penicillin acylase and to conjugated bile acid hydrolase

SEQ ID N ° 2683 lmo0447 1635.1 Unknown, similar to glutamate decarboxylase

SEQ ID N ° 2684 lmo0448 1636.1 Unknown, similar to amino acid antiporter

SEQ ID N ° 2685 lmo0459 1655.1 Unknown, similar to transcription regulator (VirR from Streptococcus pyogenes)

SEQ ID N ° 2686 lmo0460 1656.1 Unknown, putative membrane associated lipoprotein

SEQ ID N ° 2687 lmo0461 1658.1 Unknown

SEQ ID N ° 2688 lmo0462 1659.3 Unknown

SEQ ID N ° 2689 lmo0463 1660.3 Unknown

SEQ ID N ° 2690 lmo0464 3853.2 Unknown, weakly similar to transposase

SEQ ID N ⁰ 2691 lmo0465 3953.1 Hypothetical orf

SEQ ID N ° 2692 lmo0466 3905.2 Unknown

SEQ ID N ° 2693 lmo0467 3954.2 Unknown

SEQ ID N ° 2694 lmo0468 4040.1 Unknown

SEQ ID N ° 2695 lmo0469 3337.3 Unknown

SEQ ID N ° 2696 lmo0470 3336.3 Unknown, weakly similar to site-specific DNA-methyltransferase

SEQ ID N ° 2697 lmo0471 3335.2 Unknown

SEQ ID N ° 2698 lmo0472 3334.1 Unknown

SEQ ID N ° 2699 lmo0473 3333.1 Unknown

SEQ ID N ° 2700 lmo0474 3332.1 Unknown

SEQ ID N ⁰ 2701 lmo0475 3331.1 Unknown

SEQ ID N ° 2702 lmo0476 3330.2 Unknown, similar to oxetanocin A resistance protein oxrB

SEQ ID N ° 2703 lmo0477 3284.1 Unknown, putative secreted protein

SEQ ID N ° 2704 lmo0478 3285.1 Unknown, putative secreted protein

SEQ ID N ° 2705 lmo0479 3286.1 Unknown, putative secreted protein

SEQ ID N ° 2706 lmo0492 1713.1 Unknown, similar to transcriptional regulator (LysR family)

SEQ ID N ° 2707 lmo0493 1714.1 Unknown, similar to acylase

SEQ ID N ° 2708 lmo0497 1718.2 Unknown, similar to sugar transferase

SEQ ID N ° 2709 lmo0525 1037.1 Unknown

SEQ ID N ⁰ 2710 lmo0630 1518.1 Unknown, similar to transcription antiterminator BglG family

SEQ ID N ⁰ 2711 lmo0631 1519.1 Unknown, similar to PTS System, fructose-specific IIA component

SEQ ID N ⁰ 2712 lmo0632 1520.1 Unknown, similar to PTS System, fructose-specific IIC component

SEQ ID N ⁰ 2713 lmo0633 1521.1 Unknown, similar to PTS System, fructose-specific IIB component

SEQ ID N ⁰ 2714 lmo0634 1523.1 Unknown, similar to an E. coli putative tagatose 6-phosphate kinase

SEQ ID N ⁰ 2715 lmo0638 1528.1 Unknown

SEQ ID N ⁰ 2716 lmo0726 4124.1 Hypothetical CDS

SEQ ID N ⁰ 2717 lmo0733 1176.1 Unknown, similar to transcription regulator

SEQ ID N ⁰ 2718 lmo0734 1 175.1 Unknown, similar to transcriptional regulator (Lacl family)

SEQ ID N ⁰ 2719 lmo0735 1 174.1 Unknown, similar to Ribulose-5-Phosphate 3-Epimerase

SEQ ID N ° 2720 lmo0736 1 173.1 Unknown, similar to ribose 5-phosphate isomerase

SEQ ID N ⁰ 2721 lmo0737 1 172.1 Unknown

SEQ ID N ° 2722 lmo0738 1 171.1 Unknown, similar to phosphotransferase System (PTS) beta-glucoside-specific enzyme IIABC comp

SEQ ID N ° 2723 lmo0739 1 169.1 Unknown, similar to 6-phospho-beta-glucosidase

SEQ ID N ° 2724 lmo0746 1 160.1 Unknown, hypothetical

SEQ ID N ° 2725 lmo0747 1 159.1 unknown

SEQ ID N ° 2726 lmo0748 1 158.1 Unknown

SEQ ID N ° 2727 lmo0749 4361.1

SEQ ID N ° 2728 lmo0750 1 157.1 Unknown

SEQ ID N ° 2729 lmo0751 1 156.1 Unknown

SEQ ID N ° 2730 lmo0752 1 155.1 Unknown, weakly similar to a putative haloacetate dehalogenase

SEQ ID N ⁰ 2731 lmo0753 1 154.1 unknown, similar to transcription regulator Crp / Fnr family

SEQ ID N ⁰ 2732 lmo0754 1 153.2 Unknown, weakly similar to a bile acid 7-alpha dehydratase

SEQ ID N ° 2733 lmo0780 1 123.1 Unknown

SEQ ID N ° 2734 lmo0801 3477.1 Unknown, similar to intemalin, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ⁰ 2735 lmo0804 3469.2 Unknown

SEQ ID N ° 2736 lmo0805 3929.1 unknown

SEQ ID N ° 2737 lmo0826 1261.1 Unknown, similar to transport protein

SEQ ID N ° 2738 lmo0827 1259.1 unknown, similar to transposases

SEQ ID N ° 2739 lmo0828 1258.1 unknown, similar to transposases

SEQ ID N ° 2740 lmo0833 1250.1 Unknown, similar to transcriptional regulator

SEQ ID N ⁰ 2741 lmo0834 1249.1 unknown

SEQ ID N ° 2742 lmo0835 1248.1 Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2743 uhpT 1243.1 unknown, highly similar to hexose phosphate transport protein

SEQ ID N ° 2744 lmo0842 1235.1 Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2745 lmo0904 1624.2 Unknown

SEQ ID N ° 2746 lmo0933 1586.1 unknown, similar to sugar transferase

SEQ ID N ° 2747 lmo0940 1580.1 Unknown

SEQ ID N ° 2748 lmol030 3538.3 unknown, similar to transcriptional regulator, Lacl family

SEQ ID N ° 2749 lmol031 1398.3 unknown, similar to hypothetical proteins

SEQ ID N ° 2750 lmol032 1396.1 unknown, similar to transketolase

SEQ ID N ⁰ 2751 lmol033 1394.1 unknown, similar to transketolase

SEQ ID N ° 2752 lmol034 1392.1 unknown, similar to glycerol kinase

SEQ ID N ° 2753 lmol035 1391.1 unknown, similar to phosphotransferase System (PTS) beta-glucoside-specific enzyme IIABC

SEQ ID N ° 2754 lmol036 1390.1 unknown

SEQ ID N ° 2755 lmol060 1359.1 unknown, similar to transcription response regulator

SEQ ID N ° 2756 lmol061 1358.1 Unknown, similar to two-component sensor histidine kinase

SEQ ID N ° 2757 lmol062 1357.1 unknown, similar to ABC transporters (permease protein)

SEQ ID N ° 2758 lmol063 1354.1 unknown, similar to ABC transporter (ATP binding protein)

SEQ ID N ⁰ 2759 lmol076 3609.1 unknown, similar to autolysin (EC 3.5.1.28) (N-acetylmuramoyl-L-alanine amidase)

SEQ ID N ° 2760 lmol077 3612.1 unknown, similar to teichoic acid biosynthesis protein B

SEQ ID N ⁰ 2761 lmol079 3614.3 unknown, similar to B. subtilis YfhO protein

SEQ ID N ° 2762 lmol080 2597.3 unknown, similar to B. subtilis minor teichoic acids biosynthesis protein GgaB

SEQ ID N ° 2763 lmol081 2598.3 Unknown, similar to glucose- 1 -phosphate thymidyl transferase

SEQ ID N ° 2764 lmol082 2599.1 Unknown, similar to dTDP-sugar epimerase

SEQ ID N ° 2765 lmol083 2600.1 Unknown, similar to dTDP-D-glucose 4,6-dehydratase

SEQ ID N ° 2766 lmol084 2601.1 unknown, similar to DTDP-L-rhamnose synthetase

SEQ ID N ° 2767 lmol085 2602.1 unknown, similar to teichoic acid biosynthesis protein B

SEQ ID N ° 2768 lmol090 2608.1 unknown, similar to glycosyltransferases

SEQ ID N ° 2769 lmol091 2609.1 unknown, similar to glysosyltransferases

SEQ ID N ° 2770 lmol097 2618.1 unknown, similar to integrases

SEQ ID N ⁰ 2771 lmol098 4147.1 unknown, highly similar to TN916 ORF8

SEQ ID N ° 2772 lmol099 2619.1 unknown, similar to a protein encoded by Tn916

SEQ ID N ° 2773 cadA 2621.4 cadmium resistance protein

SEQ ID N ° 2774 lmol lOl 3973.2 Unknown, similar to lipoprotein signal peptidase

SEQ ID N ° 2775 lmol l02 3024.1 unknown, similar to cadmium efflux System accessory proteins

SEQ ID N ° 2776 lmol l03 3023.1 unknown, highly similar to TN916 ORF 13

SEQ ID N ° 2777 lmol l04 3022.1 unknown, highly similar to TN916 ORF 14 and to L. monocytogenes P60 protein

SEQ ID N ° 2778 lmol l05 3020.1 unknown, highly similar to TN916 ORF 15

SEQ ID N ° 2779 lmol lOό 3018.1 unknown, highly similar to TN916 ORF 16

SEQ ID N ° 2780 lmol l07 3017.1 unknown, highly similar to TN916 ORF 17

SEQ ID N ⁰ 2781 lmol l08 3016.1 unknown, highly similar to TN916 ORF 18

SEQ ID N ° 2782 lmol l09 4148.1 unknown, highly similar to TN916 ORF 19

SEQ ID N ° 2783 lmol l lO 3014.1 unknown, similar to unknown proteins

SEQ ID N ° 2784 lmol l l l 3013.1 unknown, highly similar to TN916 ORF20

SEQ ID N ° 2785 lmol l l2 3012.1 unknown, highly similar to TN916 ORF21

SEQ ID N ° 2786 lmol l l3 301 1.1 unknown, highly similar to TN916 ORF22

SEQ ID N ° 2787 lrnol l H 3010.1 unknown, highly similar to TN916 ORF23

SEQ ID N ° 2788 lmol l l5 3009.3 unknown, similar to fibrinogen-binding protein (LPXTG motif)

SEQ ID N ° 2789 lmol l lό 4267.1 unknown, similar to regulatory proteins

SEQ ID N ° 2790 lrnol l H 4268.1 unknown

SEQ ID N ⁰ 2791 lmol l lδ 4149.2 unknown

SEQ ID N ° 2792 lmol l l9 247.3 unknown, similar to methylases

SEQ ID N ° 2793 lmol l20 246.1 unknown

SEQ ID N ° 2794 lmol l21 245.1 unknown

SEQ ID N ° 2795 lmol l25 241.2 unknown

SEQ ID N ° 2796 lmol 133 232.1 unknown, similar to B. subtilis YjcS protein

SEQ ID N ° 2797 lmol l34 231.1 unknown, similar to regulatory proteins

SEQ ID N ° 2798 lmol l35 230.1 unknown

SEQ ID N ° 2799 lmol l39 223.1 unknown

SEQ ID N ° 2800 lmol l 88 156.1 unknown

SEQ ID N ⁰ 2801 lmol247 2296.1 unknown

SEQ ID N ° 2802 lmol263 4152.2 unknown, similar to transcriptional regulator

SEQ ID N ° 2803 lmol290 1974.3 Unknown, similar to intemalin proteins, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2804 lmol307 1997.1 unknown

SEQ ID N ° 2805 lmol413 1778.1 Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2806 lmol441 1814.1 Unknown, similar to putative peptidoglycan acetylation protein

SEQ ID N ° 2807 lmol451 1827.1 Unknown, similar to E. coli LytB protein

SEQ ID N ° 2808 lmol477 2928.1 Unknown, similar to oxidoreductase

SEQ ID N ° 2809 lmol478 2929.1 Unknown, similar to transcriptional regulator (MerR family)

SEQ ID N ⁰ 2810 lmol597 3951.1 Unknown

SEQ ID N ⁰ 281 1 lmol648 2130.1 unknown

SEQ ID N ⁰ 2812 lmol656 3728.1 unknown

SEQ ID N ⁰ 2813 lmo l659 3681.2 unknown

SEQ ID N ⁰ 2814 lmo 1666 3418.2 unknown, peptidoglycan linked protein (LPxTG)

SEQ ID N ⁰ 2815 lmol 714 3463.2 unknown

SEQ ID N ⁰ 2816 inlC 3779.3 intemalin C

SEQ ID N ⁰ 2817 lmo 1968 2521.1 unknown, similar to creatinine amidohydrolases

SEQ ID N ⁰ 2818 lmo 1969 2522.1 Unknown, similar to 2-keto-3-deoxygluconate-6-phosphate aldolase

SEQ ID N ⁰ 2819 lmo 1970 2523.1 Unknown, similar to putative phosphotriesterase related proteins

SEQ ID N ° 2820 lmo 1971 2524.1 Unknown, similar to pentitol PTS System enzyme II C component

SEQ ID N ⁰ 2821 lmo 1972 4065.1 Unknown, similar to pentitol PTS System enzyme II B component

SEQ ID N ° 2822 lmo 1973 2527.1 Unknown, similar to PTS System enzyme II A component

SEQ ID N ° 2823 lmo 1974 2528.1 Unknown, similar to transcription regulators, (GntR family)

SEQ ID N ° 2824 lmo2026 2504.1 Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2825 lmo2027 2503.1 Unknown, putative cell surface protein, similar to intemalin proteins

SEQ ID N ° 2826 lmo2067 3512.1 Unknown, similar to conjugated bile acid hydrolase

SEQ ID N ° 2827 lmo2085 3691.2 Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ° 2828 lmo2093 4359.1 Unknown

SEQ ID N ° 2829 lmo2143 858.1 Unknown, weakly similar to mannose-6-phosphate isomerase

SEQ ID N ° 2830 lmo2144 857.1 Unknown, similar to transcription regulator GntR family

SEQ ID N ⁰ 2831 sepA 842.1 Unknown

SEQ ID N ° 2832 lmo2197 3539.1 Unknown

SEQ ID N ° 2833 lmo2228 4342.1 Unknown, similar to unknown protein

SEQ ID N ° 2834 lmo2257 2761.1 Unknown, hypothetical CDS

SEQ ID N ° 2835 lmo2364 452.1 Hypothetical protein

SEQ ID N ° 2836 lmo2387 906.1 Unknown, conserved hypothetical protein

SEQ ID N ° 2837 lmo2395 4226.1 Unknown

SEQ ID N ° 2838 lmo2407 881.1 unknown

SEQ ID N ° 2839 lmo2408 4227.1 Unknown, similar to repressor protein

SEQ ID N ° 2840 lmo2409 880.1 Unknown

SEQ ID N ⁰ 2841 lmo2410 879.1 Unknown

SEQ ID N ° 2842 lmo2420 4358.1 unknown

SEQ ID N ° 2843 lmo2443 2215.1 unknown

SEQ ID N ° 2844 lmo2470 757.1 Unknown, similar to intemalin proteins

SEQ ID N ° 2845 lmo2576 3700.2 Unknwon, peptidoglycan anchored protein (LPXTG motif)

SEQ ID N ° 2846 lmo2594 1759.1 Unknown

SEQ ID N ° 2847 hno2595 1760.1 Unknown, similar to unknown proteins

SEQ ID N ° 2848 lmo2671 49.1 Unknown

SEQ ID N ° 2849 lmo2672 51.1 Unknown, weakly similar to transcription regulator

SEQ ID N ° 2850 lmo2686 80.1 unknown

SEQ ID N ⁰ 2851 lmo2732 270.1 unknown

SEQ ID N ° 2852 lmo2733 272.1 Unknown, similar to PTS System, fructose-specific IIABC component

SEQ ID N ° 2853 lmo2734 273.1 Unknown, weakly similar to sugar hydrolase

SEQ ID N ° 2854 lmo2735 274.1 Unknown, similar to Sucrose phosphorylase

SEQ ID N ° 2855 lmo2736 275.1 Unknown, conserved hypothetical protein

SEQ ID N ° 2856 lmo2771 710.1 Unknown, similar to beta-glucosidase

SEQ ID N ° 2857 lmo2772 71 1.2 Unknown, similar to beta-glucoside-specific enzyme IIABC

SEQ ID N ⁰ 2858 lmo2773 712.2 Unknwon, similar to transcription antiterminator

SEQ ID N ° 2859 lmo2776 716.1 Unknown

SEQ ID N ° 2860 lmo2780 721.1 Unknown, similar to cellobiose PTS enzyme IIA

SEQ ID N ⁰ 2861 lmo2781 723.1 Unknown, similar to beta-glucosidase

SEQ ID N ° 2862 lmo2782 724.1 Unknown, similar to PTS, cellobiose-specific IIB component

SEQ ID N ° 2863 lmo2783 725.1 Unknown, similar to cellobiose phosphotransferase system enzyme IIC

SEQ ID N ° 2864 lmo2784 726.1 Unknown, similar to lichenan operon transcription antiterminator licR

SEQ ID N ° 2865 bvrC 728.1 Unknown

SEQ ID N ° 2866 bvrB 730.1 beta-glucoside-specific phosphotransferase enzyme II ABC component

SEQ ID N ° 2867 bvrA 731.2 transcription antiterminator

SEQ ID N ° 2868 lmo2807 3431.2 Unknown, hypothetical secreted protein

SEQ ID N ° 2869 lmo2809 1067.2 Unknown, hypothetical secreted protein

SEQ ID N ° 2870 lmo2821 1050.1 Unknown, similar to intemalin, Unknown, putative peptidoglycan bound protein (LPXTG motif)

SEQ ID N ⁰ 2871 lmo2841 1206.1 unknown, weakly similar to sucrose phosphorylase

TABLE VIII: Legends

SEQ ID Nos. 2872 - 3891: sequences of 1020 Contigs resulting from the assembly of 13919 sequences of Listeria monocytogenes 4b.

In these sequences, the indeterminate bases are marked with an "N". Some of these contigs contain the old 974 contigs of Lm4b SEQ ID Nos. 1068 to 2041; with the SEQ ID identifier in the first column, the contig number and the sequence number (s) in the second column SEQ ID Nos. 1068 to 2041 corresponding to table V.

TABLE VIII

SEQ ID N ° 2872 Listeria monocytogenes 4b Contigl

SEQ ID N ° 2873 Listeria monocytogenes 4b Contig2

SEQ ID N ° 2874 Listeria monocytogenes 4b Contig3

SEQ ID N ° 2875 Listeria monocytogenes 4b Contig4

SEQ ID N ° 2876 Listeria monocytogenes 4b Contig5

Corresponding to the former SEQ ID n ° 1069

SEQ ID N ° 2877 Listeria monocytogenes 4b Contigό

SEQ ID N ° 2878 Listeria monocytogenes 4b Contig7

SEQ ID N ° 2879 Listeria monocytogenes 4b Contig8

Corresponding to the former SEQ ID n ° 1076

SEQ ID N ° 2880 Listeria monocytogenes 4b Contig9

SEQ ID N ° 2881 Listeria monocytogenes 4b Contig 10

SEQ ID N ° 2882 Listeria monocytogenes 4b Contigl 1

SEQ ID N ° 2883 Listeria monocytogenes 4b Contig 12

Corresponding to the former SEQ ID n ° 1075

SEQ ID N ° 2884 Listeria monocytogenes 4b Contigl 3

SEQ ID N ° 2885 Listeria monocytogenes 4b Contigl4

SEQ ID N ° 2886 Listeria monocytogenes 4b Contigl 5

SEQ ID N ° 2887 Listeria monocytogenes 4b Contigl 6

SEQ ID N ° 2888 Listeria monocytogenes 4b Contigl 7

SEQ ID N ° 2889 Listeria monocytogenes 4b Contigl 8

SEQ ID N ° 2890 Listeria monocytogenes 4b Contig 19

SEQ ID N ° 2891 Listeria monocytogenes 4b Contig20

SEQ ID N ° 2892 Listeria monocytogenes 4b Contig21

SEQ ID N ° 2893 Listeria monocytogenes 4b Contig22

SEQ ID N ° 2894 Listeria monocytogenes 4b Contig23

SEQ ID N ° 2895 Listeria monocytogenes 4b Contig24

SEQ ID N ° 2896 Listeria monocytogenes 4b Contig25

SEQ ID N ° 2897 Listeria monocytogenes 4b Contig26

SEQ ID N ° 2898 Listeria monocytogenes 4b Contig27

SEQ ID N ° 2899 Listeria monocytogenes 4b Contig28

SEQ ID N ° 2900 Listeria monocytogenes 4b Contig29

SEQ ID N ° 2901 Listeria monocytogenes 4b Contig30

SEQ ID N ° 2902 Listeria monocytogenes 4b Contig31

SEQ ID N ° 2903 Listeria monocytogenes 4b Contig32

SEQ ID N ° 2904 Listeria monocytogenes 4b Contig33

SEQ ID N ° 2905 Listeria monocytogenes 4b Contig34 SEQ ID N ° 2906 Listeria monocytogenes 4b Contig35

SEQ ID N ° 2907 Listeria monocytogenes 4b Contig36

SEQ ID N ^c 2908 Listeria monocytogenes 4b Contig37 Corresponding to the former SEQ ID n ° 1363

SEQ ID N ° 2909 Listeria monocytogenes 4b Contig38

SEQ ID N ° 2910 Listeria monocytogenes 4b Contig39

SEQ ID N ° 291 1 Listeria monocytogenes 4b Contig40 Corresponding to the former SEQ ID n ° 1 126

SEQ ID N ° 2912 Listeria monocytogenes 4b Contig41

SEQ ID N ° 2913 Listeria monocytogenes 4b Contig42

SEQ ID N ° 2914 Listeria monocytogenes 4b Contig43

SEQ ID N ° 2915 Listeria monocytogenes 4b Contig44 Corresponding to the former SEQ ID n ° 1096

SEQ ID N ° 2916 Listeria monocytogenes 4b Contig45

SEQ ID N ° 2917 Listeria monocytogenes 4b Contig46

SEQ ID N ° 2918 Listeria monocytogenes 4b Contig47

SEQ ID N ° 2919 Listeria monocytogenes 4b Contig48

SEQ ID N ° 2920 Listeria monocytogenes 4b Contig49

SEQ ID N ° 2921 Listeria monocytogenes 4b Contig50

SEQ ID N ° 2922 Listeria monocytogenes 4b Contig51 Corresponding to the former SEQ ID n ° 1 145

SEQ ID N ° 2923 Listeria monocytogenes 4b Contig52

SEQ ID N ° 2924 Listeria monocytogenes 4b Contig53

SEQ ID N ° 2925 Listeria monocytogenes 4b Contig54

SEQ ID N ° 2926 Listeria monocytogenes 4b Contig55 Corresponding to the former SEQ ID n ° 1209

SEQ ID N ° 2927 Listeria monocytogenes 4b Contig56

SEQ ID N ° 2928 Listeria monocytogenes 4b Contig57

SEQ ID N ° 2929 Listeria monocytogenes 4b Contig58

SEQ ID N ° 2930 Listeria monocytogenes 4b Contig59 Corresponding to the former SEQ ID n ° 1379

SEQ ID N ° 2931 Listeria monocytogenes 4b ContigόO

SEQ ID N ° 2932 Listeria monocytogenes 4b Contigό 1

SEQ ID N ° 2933 Listeria monocytogenes 4b Contig62

SEQ ID N ° 2934 Listeria monocytogenes 4b Contig63

SEQ ID N ° 2935 Listeria monocytogenes 4b Contig64 Corresponding to the former SEQ ID n ° 1082

SEQ ID N ° 2936 Listeria monocytogenes 4b Contig65

SEQ ID N ° 2937 Listeria monocytogenes 4b Contig66 Corresponding to the former SEQ ID n ° 1345

SEQ ID N ° 2938 Listeria monocytogenes 4b Contig67

SEQ ID N ° 2939 Listeria monocytogenes 4b Contig68

SEQ ID N ° 2940 Listeria monocytogenes 4b Contig69

SEQ ID N ° 2941 Listeria monocytogenes 4b Contig70 Corresponding to the former SEQ ID n ° 1332

SEQ ID N ° 2942 Listeria monocytogenes 4b Contig71

SEQ ID N ° 2943 Listeria monocytogenes 4b Contig72

SEQ ID N ° 2944 Listeria monocytogenes 4b Contig73

SEQ ID N ° 2945 Listeria monocytogenes 4b Contig74

SEQ ID N ° 2946 Listeria monocytogenes 4b Contig75 0 00 00 00 00 00 ÙO GO OO OO OO OO OO OO OO OO OO OO OO OO OO OO GO 00 O 00 00 00 00 00 00 00 00 00 00 00 00 00 oo mmmmmm ffl W ffl ffl ffl ffl WWWMWW ffl M ffl tl tτι mmm B tri WB m MWWWWWWWOOOO OOOOOOOOOOOOOOOOO O OO OOOO OO OOOO OOO σ z to v ©

OO so

O 00 00 00 00 00 00 00 00 0000 0000 0O Ù 0O 0O 00 000000 00 00 000000 00 00 oo oo oo oo oo oo oo O OO 00 call in tri mmmmm tfl en en en en en en en en rn en en en en en en en en en WW tu w rt tπ W en en fπ

O O OOOOOOOOOOO OOOOO O OOO O O OOOOO OOOOOOO OO O

ODD Oϋ O ϋ ϋ OO ϋ OO O ϋ ϋ OOO ϋ ODOOO ϋ ODDUODOOOO ϋ ϋ O zz zz oz 0z 0z 0z 0z 0zzzz zzzzz z zzz zz zzzzz zzzzzzz zz z> w <_> J 1 J w e-) w W ej w) wwwww i- WW ιj J uo to to to to to to to to to to to to io to oo OOOOO "O - * O _* - - OOOOOOOOOOOOOO ^ ^ o ^ oo" \ O> O Ό Φ ^ \ O \ © V © ^ O t to to to to - _^ - • - _ _- -. _. ^ - o OOOOOOOOO ^ O * © ^ O <© V © © v ©> © © \ © oo OO OO OO ^ J tO o * © 00 - ~ J C5> ι ι ^ w to - o Φ oo -J os υi J- ω to> - oo oo ι α J ww - o Ό OO -JO \

SEQ ID N ° 3025 Listeria monocytogenes 4b Contigl 54

SEQ ID N ° 3026 Listeria monocytogenes 4b Contigl 55 SEQ ID N ° 3027 Listeria monocytogenes 4b Contigl 56 SEQ ID N ° 3028 Listeria monocytogenes 4b Contigl 57

Corresponding to the former SEQ ID n ° 1 107 SEQ ID N ° 3029 Listeria monocytogenes 4b Contigl 58 SEQ ID N ° 3030 Listeria monocytogenes 4b Contigl 59 SEQ ID N ° 3031 Listeria monocytogenes 4b Contigl 60

SEQ ID N ° 3032 Listeria monocytogenes 4b Contigl 61

Corresponding to the former SEQ ID n ° 1485 SEQ ID N ° 3033 Listeria monocytogenes 4b Contigl 62

SEQ ID N ° 3034 Listeria monocytogenes 4b Contigl 63 SEQ ID N ° 3035 Listeria monocytogenes 4b Contig 164 SEQ ID N ° 3036 Listeria monocytogenes 4b Contigl 65

Corresponding to the former SEQ ID n ° 1538 SEQ ID N ° 3037 Listeria monocytogenes 4b Contigl 66

Corresponding to the former SEQ ID n ° 1409 SEQ ID N ° 3038 Listeria monocytogenes 4b Contig 167

SEQ ID N ° 3039 Listeria monocytogenes 4b Contigl 68 SEQ ID N ° 3040 Listeria monocytogenes 4b Contigl 69 SEQ ID N ° 3041 Listeria monocytogenes 4b Contigl 70

SEQ ID N ° 3042 Listeria monocytogenes 4b Contigl 71

Corresponding to the foπΗer SEQ ID No. 1246 SEQ ID No. 3043 Listeria monocytogenes 4b Contigl 72

SEQ ID N ° 3044 Listeria monocytogenes 4b Contigl 73 SEQ ID N ° 3045 Listeria monocytogenes 4b Contig 174

SEQ ID N ° 3046 Listeria monocytogenes 4b Contig 175

Corresponding to the former SEQ ID n ° 1445 SEQ ID N ° 3047 Listeria monocytogenes 4b Contig 176 SEQ ID N ° 3048 Listeria monocytogenes 4b Contig 177 SEQ ID N ° 3049 Listeria monocytogenes 4b Contigl 78 SEQ ID N ° 3050 Listeria monocytogenes 4b Contig 179

Corresponding to the former SEQ ID No. 1326 SEQ ID No. 3051 Listeria monocytogenes 4b Contig 180

Corresponding to the former SEQ ID n ° 1590 SEQ ID N ° 3052 Listeria monocytogenes 4b Contigl 81 SEQ ID N ° 3053 Listeria monocytogenes 4b Contig 182

Corresponding to the former SEQ ID n ° 1 152 SEQ ID N ° 3054 Listeria monocytogenes 4b Contigl 83 SEQ ID N ° 3055 Listeria monocytogenes 4b Contigl 84 SEQ ID N ° 3056 Listeria monocytogenes 4b Contigl 85 SEQ ID N ° 3057 Listeria monocytogenes 4b Contigl 86

Corresponding to the former SEQ ID n ° 1550 SEQ ID N ° 3058 Listeria monocytogenes 4b Contigl 87 SEQ ID N ° 3059 Listeria monocytogenes 4b Contigl 88 SEQ ID N ° 3060 Listeria monocytogenes 4b Contigl 89 SEQ ID N ° 3061 Listeria monocytogenes 4b Contigl 90

SEQ ID N ° 3062 Listeria monocytogenes 4b Contigl 91 SEQ ID N ⁰ 3063 Listeria monocytogenes 4b Contig 192

SEQ ID N ° 3064 Listeria monocytogenes 4b Contigl 93 SEQ ID N ° 3065 Listeria monocytogenes 4b Contigl 94

Corresponding to the former SEQ ID n ° 1535 SEQ ID N ° 3066 Listeria monocytogenes 4b Contigl 95 SEQ ID N ° 3067 Listeria monocytogenes 4b Contigl 96

Corresponding to the former SEQ ID n ° 1630 SEQ ID N ° 3068 Listeria monocytogenes 4b Contigl 97

SEQ ID N ° 3069 Listeria monocytogenes 4b Contig 198

SEQ ID N ° 3070 Listeria monocytogenes 4b Contigl 99 SEQ ID N ° 3071 Listeria monocytogenes 4b Contig200

SEQ ID N ° 3072 Listeria monocytogenes 4b Contig201 SEQ ID N ° 3073 Listeria monocytogenes 4b Contig202

Corresponding to the former SEQ ID No. 1436 SEQ ID No. 3074 Listeria monocytogenes 4b Contig203 SEQ ID No. 3075 Listeria monocytogenes 4b Contig204

SEQ ID N ° 3076 Listeria monocytogenes 4b Contig205

Corresponding to the former SEQ ID No. 1267 SEQ ID No. 3077 Listeria monocytogenes 4b Contig206

Corresponding to the former SEQ ID No. 1461 SEQ ID No. 3078 Listeria monocytogenes 4b Contig207

SEQ ID N ° 3079 Listeria monocytogenes 4b Contig208

Corresponding to the former SEQ ID n ° 1 168 SEQ ID N ° 3080 Listeria monocytogenes 4b Contig209 SEQ ID N ° 3081 Listeria monocytogenes 4b Contig210

SEQ ID N ° 3082 Listeria monocytogenes 4b Contig21 1

Corresponding to the former SEQ ID No. 1 159 SEQ ID No. 3083 Listeria monocytogenes 4b Contig212

SEQ ID N ° 3084 Listeria monocytogenes 4b Contig213 SEQ ID N ° 3085 Listeria monocytogenes 4b Contig214

SEQ ID N ° 3086 Listeria monocytogenes 4b Contig215 SEQ ID N ° 3087 Listeria monocytogenes 4b Contig216

SEQ ID N ° 3088 Listeria monocytogenes 4b Contig217

SEQ ID N ° 3089 Listeria monocytogenes 4b Contig218

Corresponding to the former SEQ ID n ° 1206 SEQ ID N ° 3090 Listeria monocytogenes 4b Contig219

Corresponding to the former SEQ ID No. 1618 SEQ ID No. 3091 Listeria monocytogenes 4b Contig220

Corresponding to the former SEQ ID n ° 1540 SEQ ID N ° 3092 Listeria monocytogenes 4b Contig221

Corresponding to the former SEQ ID n ° 1568 SEQ ID N ° 3093 Listeria monocytogenes 4b Contig222

Corresponding to the former SEQ ID No. 1084 SEQ ID No. 3094 Listeria monocytogenes 4b Contig223 SEQ ID No. 3095 Listeria monocytogenes 4b Contig224

SEQ ID N ° 3096 Listeria monocytogenes 4b Contig225

SEQ ID N ° 3097 Listeria monocytogenes 4b Contig226

Corresponding to the former SEQ ID n ° 1464

SEQ ID N ° 3098 Listeria monocytogenes 4b Contig227

SEQ ID N ° 3099 Listeria monocytogenes 4b Contig228 SEQ ID N ° 3100 Listeria monocytogenes 4b Contig229 SEQ ID N ° 3101 Listeria monocytogenes 4b Contig230 oo oo oo oo oo oo oo oo oo oo oo mmmmmmmmmmmmmmmmmmmmm mmmmmmm eπ ert efl eπ en en en in MW en en en en en en en en en en M w ed en êd en en en en en en eπ en en en in

OOOO OO O OO O OOOO OOOOOOO OOOOOO OOOOO OO OO OO

Doσo UO u σo oo σσσσ σσσσσσσ OUOOOO σσσσα oσ σσ σσ z ozozozo z ozo zoz ozo z ozo z ozozozo z ozozozozozozo z ozozozozozo z OzOzOzOzO z OzO z OzO> w ω ω w>> w> ω w> ω w> ω w> ω w> ω w ω uww ω> w> w> W ω w ω>>

4- w> w o O O O O O O O

OO oo i Φ oo -J OΛ eyi 4- υ to

l / i>-> - tO> - 4- OO 4-. J 4-.

- t- CJ C7 \ ^ tO Os t -O W

I H- t ^ i 0 ~ 0 00 ^ - 1—, - ~ -j, __.

SEQ ID N ° 3141 Listeria monocytogenes 4b Contig270

Corresponding to the former SEQ ID n ° 1710 SEQ ID N ° 3142 Listeria monocytogenes 4b Contig271

SEQ ID N ° 3143 Listeria monocytogenes 4b Contig272

Corresponding to the former SEQ ID No. 1450 SEQ ID No. 3144 Listeria monocytogenes 4b Contig273

Corresponding to the former SEQ ID n ° 1472 SEQ ID N ° 3145 Listeria monocytogenes 4b Contig274

Corresponding to the former SEQ ID n ° 1276 SEQ ID N ° 3146 Listeria monocytogenes 4b Contig275 SEQ ID N ° 3147 Listeria monocytogenes 4b Contig276 SEQ ID N ° 3148 Listeria monocytogenes 4b Contig277

Corresponding to the former SEQ ID n ° 1524 SEQ ID N ° 3149 Listeria monocytogenes 4b Contig278 SEQ ID N ° 3150 Listeria monocytogenes 4b Contig279 SEQ ID N ° 3151 Listeria monocytogenes 4b Contig280

Corresponding to the former SEQ ID n ° 1 171 SEQ ID N ° 3152 Listeria monocytogenes 4b Contig281

Corresponding to the former SEQ ID n ° 1528 SEQ ID N ° 3153 Listeria monocytogenes 4b Contig282

SEQ ID N ° 3154 Listeria monocytogenes 4b Contig283 SEQ ID N ° 3155 Listeria monocytogenes 4b Contig284

Corresponding to the former SEQ ID n ° 1570 SEQ ID N ° 3156 Listeria monocytogenes 4b Contig285 SEQ ID N ° 3157 Listeria monocytogenes 4b Contig286 SEQ ID N ° 3158 Listeria monocytogenes 4b Contig287

Corresponding to the former SEQ ID No. 1435 SEQ ID No. 3159 Listeria monocytogenes 4b Contig288 SEQ ID No. 3160 Listeria monocytogenes 4b Contig289

Corresponding to the former SEQ ID No. 1659 SEQ ID No. 3161 Listeria monocytogenes 4b Contig290

SEQ ID N ° 3162 Listeria monocytogenes 4b Contig291

SEQ ID N ° 3163 Listeria monocytogenes 4b Contig292

SEQ ID N ° 3164 Listeria monocytogenes 4b Contig293 SEQ ID N ° 3165 Listeria monocytogenes 4b Contig294

SEQ ID N ° 3166 Listeria monocytogenes 4b Contig295 SEQ ID N ° 3167 Listeria monocytogenes 4b Contig296

SEQ ID N ° 3168 Listeria monocytogenes 4b Contig297

Corresponding to the former SEQ ID n ° 1500 SEQ ID N ° 3169 Listeria monocytogenes 4b Contig298 SEQ ID N ° 3170 Listeria monocytogenes 4b Contig299

Corresponding to the former SEQ ID No. 1668 SEQ ID No. 3171 Listeria monocytogenes 4b Contig300

SEQ ID N ° 3172 Listeria monocytogenes 4b Contig301

Corresponding to the former SEQ ID No. 1091 SEQ ID No. 3173 Listeria monocytogenes 4b Contig302

SEQ ID N ° 3174 Listeria monocytogenes 4b Contig303

Corresponding to the former SEQ ID No. 1338 SEQ ID No. 3175 Listeria monocytogenes 4b Contig304

Corresponding to the former SEQ ID No.1109 o

O

H U α.

iΛ φ

O O

. 'P≥P .'P2? CC

O Y ou i -O

O n cn in <u cu

_' - * ucuc

0000

- * o- ^» + o-

2 O 2O c c

O O ε σ3 εcβ

£ *! _) Ω ω w

P p

OO WHERE OOO - <mmm zz z

90 90 Û O Q Û Q Q Û Q O Q Û Q Q Q O Ù O Q O Q Q Q Q Q Q Q Q O Q Q Q Û Q Q O

O o momomoω o woωowow o W WooP-oWoW o m o ωom o ωow o ω ωoω o p o ωom o woω o m o ωowoω popoωom o w

O mmmmmmmmmmmmmmmmmmmmm mmm oo

SEQ ID N ° 321 1 Listeria monocytogenes 4b Contig340

Corresponding to the former SEQ ID No. 1262 SEQ ID No. 3212 Listeria monocytogenes 4b Contig341 SEQ ID No. 3213 Listeria monocytogenes 4b Contig342

SEQ ID No. 3214 Listeria monocytogenes 4b Contig343 SEQ ID No. 3215 Listeria monocytogenes 4b Contig344

Corresponding to the former SEQ ID No. 1662 SEQ ID No. 3216 Listeria monocytogenes 4b Contig345

Corresponding to the former SEQ ID No. 1215 SEQ ID No. 3217 Listeria monocytogenes 4b Contig346

Corresponding to the former SEQ ID No. 1350 SEQ ID No. 3218 Listeria monocytogenes 4b Contig347

Corresponding to the former SEQ ID No.1101

SEQ ID ⁰ 3219 Listeria monocytogenes 4b Contig348

Corresponding to the former SEQ ID n ° 1 162 SEQ ID n ° 3220 Listeria monocytogenes 4b Contig349

Corresponding to the former SEQ ID n ° 1251 SEQ ID N ⁰ 3221 Listeria monocytogenes 4b Contig350

Corresponding to the former SEQ ID n ° 1696 S _^ .E _^ Q ^ ID N .. ° 3 _2. 22 Listeria monocytogenes 4b Contig351

SEQ ID No. 3223 Listeria monocytogenes 4b Contig352

SEQ ID N ° 3224 Listeria monocytogenes 4b Contig353 SEQ ID N ° 3225 Listeria monocytogenes 4b Contig354

SEQ ID N ° 3226 Listeria monocytogenes 4b Contig355 SEQ ID N ° 3227 Listeria monocytogenes 4b Contig356

Corresponding to the former SEQ ID n ° 1248

SEQ ID N ° 3228 Listeria monocytogenes 4b Contig357

Corresponding to the former SEQ ID n ° 1849 SEQ ID N ° 3229 Listeria monocytogenes 4b Contig358

Corresponding to the former SEQ ID No. 1229 SEQ ID No. 3230 Listeria monocytogenes 4b Contig359

Corresponding to the foπner SEQ ID No. 1858

SEQ ID N ⁰ 323 Listeria monocytogenes 4b Contig360

Corresponding to the former SEQ ID n ° 1270

„S„ EQ ^. I „D N.. ° 3232 Listeria monocytogenes 4b Contig361

SEQ ID N ° 3233 Listeria monocytogenes 4b Contig362

Corresponding to the former SEQ ID n ° 1862 SEQ ID N ° 3234 Listeria monocytogenes 4b Contig363

Corresponding to the former SEQ ID n ° 1078 SEQ ID N ° 3235 Listeria monocytogenes 4b Contig364

Corresponding to the former SEQ ID n ° 1090 SEQ ID N ° 3236 Listeria monocytogenes 4b Contig365 SEQ ID N ° 3237 Listeria monocytogenes 4b Contig366 SEQ ID N ° 3238 Listeria monocytogenes 4b Contig367

Corresponding to the former SEQ ID n ° 1 1 15 SEQ ID N ° 3239 Listeria monocytogenes 4b Contig368 SEQ ID N ° 3240 Listeria monocytogenes 4b Contig369 SEQ ID N ° 3241 Listeria monocytogenes 4b Contig370

Corresponding to the former SEQ ID n ° 1741 SEQ ID N ° 3242 Listeria monocytogenes 4b Contig371 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 en en en en en en en en en en en en en en en en en en en en en en m en en en

O OO O o O OO O O O O O OO OOOOO OOOOO O OO

BO o DDO α o σ α σ DO α α α O OOϋOϋ OϋOOϋ σ o Ό 8 zozoz ZZ ozzozzozoz Z zozzzzo zzzzz z ozzzz ozoz oz ozt ro

On 4 ^ on ^ 00 oo o to e »00 N © to to ro to 00 Os ON r ON ro

o 00 OO 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 en en en en en en en en en en en en en en en en en en en en en en en en en en

OO OOO OO OO OO O OO OOO OOOO OO OO OO O OO O σ σ OOD σ oa σ σ σ OD σ σ α O σ oooo σ σ σ o σ σ σ o σ σ zoz 0 Z o Z 0 Z oz 0 z 0 z 0z 0 z 0 z 0 Z 0 z 0 z 0 z 0 z 0 z 0 z 0 z 0z 0z 0z 0 zozo oz 0 z 0z 0 z 0 z 0 z 0 0 0

OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ OJ J

OJ OJ J OJ OJ OJ to to ro to t ro to ro to to t t t to ro to ro ro to to ro to t to ro t

OOOOON © NO N © N © N © NO N © VO NO N © oo oo 00 oo 00 00 oo 00 00 00 ~ j ^ 1 -J -J -j ^ J on 42- OJ t N © 00 -J ON on 42- OJ ro ON © 00 ^ on 42- OJ to O NO 00 ^ J ON on 42-

O O

ON ro on to on ro ON to on 42. OJ ON N © oo ro NO 00 O o on 42- OJ 00 N © on OJ ro OJ ro on on OJ oo

Corresponding to the former SEQ ID No. 1665 SEQ ID No. 3306 Listeria monocytogenes 4b Contig435

Corresponding to the former SEQ ID n ° 1243 Corresponding to the former SEQ ID n ° 1431 SEQ ID N ° 3307 Listeria monocytogenes 4b Contig436 SEQ ID N ° 3308 Listeria monocytogenes 4b Contig437 SEQ ID N ° 3309 Listeria monocytogenes 4b Contig438 SEQ ID N ° 3310 Listeria monocytogenes 4b Contig439

Corresponding to the former SEQ ID n ° 1615 SEQ ID N ° 331 1 Listeria monocytogenes 4b Contig440

Corresponding to the former SEQ ID No. 1074 Corresponding to the former SEQ ID No. 1518 SEQ ID No. 3312 Listeria monocytogenes 4b Contig441

Corresponding to the former SEQ ID n ° 1 1 18 SEQ ID N ° 3313 Listeria monocytogenes 4b Contig442

Corresponding to the former SEQ ID No. 1692 SEQ ID No. 3314 Listeria monocytogenes 4b Contig443

Corresponding to the former SEQ ID No. 1255 SEQ ID No. 3315 Listeria monocytogenes 4b Contig444 SEQ ID No. 3316 Listeria monocytogenes 4b Contig445

Corresponding to the former SEQ ID n ° 1283 SEQ ID N ° 3317 Listeria monocytogenes 4b Contig446

Corresponding to the former SEQ ID n ° 1387 SEQ ID N ° 3318 Listeria monocytogenes 4b Contig447 SEQ ID N ° 3319 Listeria monocytogenes 4b Contig448

Corresponding to the former SEQ ID No. 1953 SEQ ID No. 3320 Listeria monocytogenes 4b Contig449 SEQ ID No. 3321 Listeria monocytogenes 4b Contig450

SEQ ID N ° 3322 Listeria monocytogenes 4b Contig451

Corresponding to the former SEQ ID n ° 1714 SEQ ID N ° 3323 Listeria monocytogenes 4b Contig452

SEQ ID N ° 3324 Listeria monocytogenes 4b Contig453 SEQ ID N ° 3325 Listeria monocytogenes 4b Contig454

SEQ ID N ° 3326 Listeria monocytogenes 4b Contig455 SEQ ID N ° 3327 Listeria monocytogenes 4b Contig456

SEQ ID N ° 3328 Listeria monocytogenes 4b Contig457 SEQ ID N ° 3329 Listeria monocytogenes 4b Contig458

Corresponding to the former SEQ ID n ° 1208

SEQ ID N ° 3330 Listeria monocytogenes 4b Contig459 Corresponding to the former SEQ ID n ° 1403 SEQ ID N ⁰ 3331 Listeria monocytogenes 4b Contig460 Corresponding to the former SEQ ID n ° 1443 Corresponding to the former SEQ ID n ° 1558 SEQ ID N ° 3332 Listeria monocytogenes 4b Contig461

Corresponding to the former SEQ ID n ° 1323

SEQ ID N ⁰ 3333 Listeria monocytogenes 4b Contig462 Corresponding to the former SEQ ID n ° 1 184 - S. _^ EQ ^ I .D _^ N. , ° - 3, .3, -3.4. Listeria monocytogenes 4b Contig463

SEQ ID N ° 3335 Listeria monocytogenes 4b Contig464 SEQ ID N ° 3336 Listeria monocytogenes 4b Contig465 Corresponding to the former SEQ ID No. 1274 SEQ ID No. 3337 Listeria monocytogenes 4b Contig466

Corresponding to the former SEQ ID n ° 1815 SEQ ID N ° 3338 Listeria monocytogenes 4b Contig467

Corresponding to the former SEQ ID n ° 1607 SEQ ID N ° 3339 Listeria monocytogenes 4b Contig468

Corresponding to the former SEQ ID n ° 1573 SEQ ID N ° 3340 Listeria monocytogenes 4b Contig469 SEQ ID N ° 3341 Listeria monocytogenes 4b Contig470

Corresponding to the former SEQ ID n ° 1737 SEQ ID N ° 3342 Listeria monocytogenes 4b Contig471

Corresponding to the former SEQ ID n ° 1404 SEQ ID N ° 3343 Listeria monocytogenes 4b Contig472

Corresponding to the former SEQ ID n ° 1344 SEQ ID N ° 3344 Listeria monocytogenes 4b Contig473

Corresponding to the former SEQ ID No.1197

Corresponding to the former SEQ ID n ° 1490 SEQ ID N ° 3345 Listeria monocytogenes 4b Contig474

SEQ ID N ° 3346 Listeria monocytogenes 4b Contig475

Corresponding to the former SEQ ID n ° 1346 SEQ ID N ° 3347 Listeria monocytogenes 4b Contig476

Corresponding to the former SEQ ID n ° 1 1 12 SEQ ID N ° 3348 Listeria monocytogenes 4b Contig477 SEQ ID N ° 3349 Listeria monocytogenes 4b Contig478 SEQ ID N ° 3350 Listeria monocytogenes 4b Contig479 SEQ ID N ° 3351 Listeria monocytogenes 4b Contig480

SEQ ID N ° 3352 Listeria monocytogenes 4b Contig481

Corresponding to the former SEQ ID No. 1213 SEQ ID No. 3353 Listeria monocytogenes 4b Contig482

Corresponding to the former SEQ ID n ° 1 124 SEQ ID n ° 3354 Listeria monocytogenes 4b Contig483

Corresponding to the former SEQ ID No. 1250 SEQ ID No. 3355 Listeria monocytogenes 4b Contig484 SEQ ID No. 3356 Listeria monocytogenes 4b Contig485

Corresponding to the former SEQ ID No. 1362 SEQ ÏD No. 3357 Listeria monocytogenes 4b Contig486

Corresponding to the former SEQ ID n ° 1408 SEQ ID N ° 3358 Listeria monocytogenes 4b Contig487

SEQ ID N ° 3359 Listeria monocytogenes 4b Contig488

Corresponding to the former SEQ ID No. 1331 SEQ ID No. 3360 Listeria monocytogenes 4b Contig489 SEQ ID No. 3361 Listeria monocytogenes 4b Contig490

Corresponding to the former SEQ ID n ° 1596 SEQ ID N ° 3362 Listeria monocytogenes 4b Contig491

Corresponding to the former SEQ ID n ° 1537 SEQ ID N ° 3363 Listeria monocytogenes 4b Contig492

SEQ ID N ° 3364 Listeria monocytogenes 4b Contig493

Corresponding to the former SEQ ID No. 1503 SEQ ID No. 3365 Listeria monocytogenes 4b Contig494

Corresponding to the former SEQ ID n ° 1915 SEQ ID N ° 3366 Listeria monocytogenes 4b Contig495 SEQ ID N ° 3367 Listeria monocytogenes 4b Contig496 SEQ ID N ° 3368 Listeria monocytogenes 4b Contig497 SEQ ID N ° 3369 Listeria monocytogenes 4b Contig498 Corresponding to the former SEQ ID n ° 476

SEQ ID N ° 3370 Listeria monocytogenes 4b Contig499 Corresponding to the former SEQ ID n ° 871

SEQ ID N ⁰ 3371 Listeria monocytogenes 4b Contig500 Corresponding to the former SEQ ID n ° 071 Corresponding to the former SEQ ID n ° 526

SEQ ID N ° 3372 Listeria monocytogenes 4b Contig501 Corresponding to the former SEQ ID n ° 324

SEQ ID N ° 3373 Listeria monocytogenes 4b Contig502 Corresponding to the former SEQ ID n ° 709

SEQ ID N ° 3374 Listeria monocytogenes 4b Contig503 SEQ ID N ° 3375 Listeria monocytogenes 4b Contig504 Corresponding to the former SEQ ID n ° 172

SEQ ID N ° 3376 Listeria monocytogenes 4b Contig505 SEQ ID N ° 3377 Listeria monocytogenes 4b Contig506 Corresponding to the former SEQ ID n ° 077

SEQ ID N ° 3378 Listeria monocytogenes 4b Contig507 Corresponding to the former SEQ ID n ° 329

SEQ ID N ° 3379 Listeria monocytogenes 4b Contig508 Corresponding to the former SEQ ID n ° 416

SEQ ID N ⁰ 3380 Listeria monocytogenes 4b Contig509 Corresponding to the former SEQ ID n ° 763

SEQ ID N ⁰ 3381 Listeria monocytogenes 4b Contig510 Corresponding to the former SEQ ID n ° 916

SEQ ID N ° 3382 Listeria monocytogenes 4b Contig51 1 Corresponding to the former SEQ ID n ° 285

SEQ ID N ⁰ 3383 Listeria monocytogenes 4b Contig512 Corresponding to the former SEQ ID n ° 298

SEQ ID N ° 3384 Listeria monocytogenes 4b Contig513 SEQ ID N ° 3385 Listeria monocytogenes 4b Contig514 Corresponding to the former SEQ ID n ° 237

SEQ ID N ° 3386 Listeria monocytogenes 4b Contig515 SEQ ID N ° 3387 Listeria monocytogenes 4b Contig516 SEQ ID N ° 3388 Listeria monocytogenes 4b Contig517 Corresponding to the former SEQ ID n ° 658

SEQ ID ⁰ 3389 Listeria monocytogenes 4b Contig518 SEQ ID N ° 3390 Listeria monocytogenes 4b Contig519 Corresponding to the former SEQ ID n ° 454

SEQ ID N ⁰ 3391 Listeria monocytogenes 4b Contig520 Corresponding to the former SEQ ID n ° 351

SEQ ID N ° 3392 Listeria monocytogenes 4b Contig521 Corresponding to the former SEQ ID n ° 148

SEQ ID N ° 3393 Listeria monocytogenes 4b Contig522 Corresponding to the former SEQ ID n ° 456

SEQ ID N ° 3394 Listeria monocytogenes 4b Contig523 Corresponding to the former SEQ ID n ° 899 SEQ ID N ° 3395 Listeria monocytogenes 4b Contig524 Corresponding to the former SEQ ID n ° 847

SEQ ID N ° 3396 Listeria monocytogenes 4b Contig525 Corresponding to the former SEQ ID n ° 639

SEQ ID N ° 3397 Listeria monocytogenes 4b Contig526 Corresponding to the former SEQ ID n ° 663

SEQ ID N ⁰ 3398 Listeria monocytogenes 4b Contig527 Corresponding to the former SEQ ID n ° 093

SEQ ID N ⁰ 3399 Listeria monocytogenes 4b Contig528 Corresponding to the former SEQ ID n ° 193

SEQ ID N ° 3400 Listeria monocytogenes 4b Contig529 Corresponding to the former SEQ ID n ° 280

SEQ ID N ⁰ 3401 Listeria monocytogenes 4b Contig530 Corresponding to the former SEQ ID n ° 143

SEQ ID N ° 3402 Listeria monocytogenes 4b Contig531 Corresponding to the former SEQ ID n ° 499

SEQ ID N ° 3403 Listeria monocytogenes 4b Contig532 Corresponding to the former SEQ ID n ° 627

SEQ ID N ° 3404 Listeria monocytogenes 4b Contig533 SEQ ID N ° 3405 Listeria monocytogenes 4b Contig534 SEQ ID N ° 3406 Listeria monocytogenes 4b Contig535 Corresponding to the former SEQ ID n ° 133

SEQ ID N ° 3407 Listeria monocytogenes 4b Contig536 Corresponding to the former SEQ ID n ° 163

SEQ ID N ° 3408 Listeria monocytogenes 4b Contig537 Corresponding to the former SEQ ID n ° 135

SEQ ID N ° 3409 Listeria monocytogenes 4b Contig538 SEQ ID N ⁰ 3410 Listeria monocytogenes 4b Contig539 Corresponding to the former SEQ ID n ° 838

SEQ ID N ⁰ 341 1 Listeria monocytogenes 4b Contig540 Corresponding to the former SEQ ID n ° 217

SEQ ID N ⁰ 3412 Listeria monocytogenes 4b Contig541 Corresponding to the former SEQ ID n ° 297

SEQ ID N ⁰ 3413 Listeria monocytogenes 4b Contig542 SEQ ID N ⁰ 3414 Listeria monocytogenes 4b Contig543 Corresponding to the former SEQ ID n ° 765

SEQ ID N ⁰ 3415 Listeria monocytogenes 4b Contig544 Corresponding to the former SEQ ID n ° 228

SEQ ID N ⁰ 3416 Listeria monocytogenes 4b Contig545 Corresponding to the former SEQ ID n ° 638

SEQ ID N ⁰ 3417 Listeria monocytogenes 4b Contig546 Corresponding to the former SEQ ID n ° 965

SEQ ID N ⁰ 3418 Listeria monocytogenes 4b Contig547 Corresponding to the former SEQ ID n ° 211

SEQ ID N ⁰ 3419 Listeria monocytogenes 4b Contig548 Corresponding to the former SEQ ID n ° 327

SEQ ID No. 3420 Listeria monocytogenes 4b Contig549 Corresponding to the foπner SEQ ID No. 502

SEQ ID N ⁰ 3421 Listeria monocytogenes 4b Contig550 Corresponding to the former SEQ ID n ° 252 SEQ ID N ° 3422 Listeria monocytogenes 4b Contig551

Corresponding to the former SEQ ID n ° 1721

SEQ ID N ° 3423 Listeria monocytogenes 4b Contig552 Corresponding to the former SEQ ID n ° 1349 Corresponding to the former SEQ ID n ° 1728

SEQ ID N ° 3424 Listeria monocytogenes 4b Contig553

Corresponding to the former SEQ ID n ° 1691

SEQ ID N ° 3425 Listeria monocytogenes 4b Contig554

Corresponding to the former SEQ ID No. 1227 Corresponding to the former SEQ ID No. 1399

SEQ ID N ° 3426 Listeria monocytogenes 4b Contig555

Corresponding to the former SEQ ID n ° 1555

SEQ ID N ° 3427 Listeria monocytogenes 4b Contig556

SEQ ID N ° 3428 Listeria monocytogenes 4b Contig557

Corresponding to the former SEQ ID No. 1 1 14 Corresponding to the former SEQ ID No. 1366

SEQ ID N ° 3429 Listeria monocytogenes 4b Contig558

SEQ ID N ° 3430 Listeria monocytogenes 4b Contig559

Corresponding to the former SEQ ÏD n ° 1805

SEQ ID N ° 343 Listeria monocytogenes 4b Contig560

Corresponding to the former SEQ ID No. 1852 SEQ _^ ID N. . ° 3 „432 Listeria monocytogenes 4b Contig561

SEQ ID N ° 3433 Listeria monocytogenes 4b Contig562

Corresponding to the former SEQ ID n ° 1923 SEQ ID N ° 3434 Listeria monocytogenes 4b Contig563

Corresponding to the former SEQ ID n ° 1395

SEQ ID N ° 3435 Listeria monocytogenes 4b Contig564

Corresponding to the former SEQ ID n ° 1561

SEQ ID N ° 3436 Listeria monocytogenes 4b Contig565

SEQ ID N ° 3437 Listeria monocytogenes 4b Contig566

Corresponding to the former SEQ ID n ° 1643

SEQ ID No. 3438 Listeria monocytogenes 4b Contig567 Corresponding to the former SEQ ID No. 1820 SEQ ID No. 3439 Listeria monocytogenes 4b Contig568 Corresponding to the former SEQ ID No. 1177 S „E ^ Q ^ ID No. .. - .4.4.0 „Listeria monocytogenes 4b Contig569

SEQ ID N ° 3441 Listeria monocytogenes 4b Contig570

Corresponding to the former SEQ ID n ° 1501

SEQ ID N ° 3442 Listeria monocytogenes 4b Contig571

Corresponding to the former SEQ ID n ° 1 195

SEQ ID N ° 3443 Listeria monocytogenes 4b Contig572

Corresponding to the former SEQ ID n ° 1556

SEQ ID N ° 3444 Listeria monocytogenes 4b Contig573

SEQ ID N ° 3445 Listeria monocytogenes 4b Contig574

Corresponding to the former SEQ ID n ° 1888

SEQ ID N ° 3446 Listeria monocytogenes 4b Contig575

Corresponding to the former SEQ ID n ° 1730

SEQ ID N ° 3447 Listeria monocytogenes 4b Contig576

Corresponding to the former SEQ ID n ° 1629

SEQ ID N ° 3448 Listeria monocytogenes 4b Contig577 Corresponding to the former SEQ ID No. 1688 SEQ ID No. 3449 Listeria monocytogenes 4b Contig578

Corresponding to the former SEQ ID n ° 1549 SEQ ID N ° 3450 Listeria monocytogenes 4b Contig579

Corresponding to the former SEQ ID n ° 1673 SEQ ID N ° 3451 Listeria monocytogenes 4b Contig580

Corresponding to the fornier SEQ ID No. 1273 SEQ ID No. 3452 Listeria monocytogenes 4b Contig581

Corresponding to the former SEQ ID No. 1415 SEQ ID No. 3453 Listeria monocytogenes 4b Contig582

SEQ ID N ° 3454 Listeria monocytogenes 4b Contig583

Corresponding to the former SEQ ID n ° 1281 SEQ ID N ° 3455 Listeria monocytogenes 4b Contig584

Corresponding to the former SEQ ID n ° 1572 SEQ ID N ° 3456 Listeria monocytogenes 4b Contig585 SEQ ID N ° 3457 Listeria monocytogenes 4b Contig586

Corresponding to the former SEQ ID n ° 1949 SEQ ID N ° 3458 Listeria monocytogenes 4b Contig587

Corresponding to the former SEQ ID No. 1625 SEQ ID No. 3459 Listeria monocytogenes 4b Contig588 SEQ ID No. 3460 Listeria monocytogenes 4b Contig589

Corresponding to the former SEQ ID n ° 1622 SEQ ID N ° 3461 Listeria monocytogenes 4b Contig590

Corresponding to the former SEQ ID No. 1086 SEQ ID No. 3462 Listeria monocytogenes 4b Contig591

Corresponding to the former SEQ ID n ° 1781 SEQ ID N ° 3463 Listeria monocytogenes 4b Contig592

Corresponding to the former SEQ ID n ° 1304 SEQ ID N ° 3464 Listeria monocytogenes 4b Contig593

Corresponding to the foπner SEQ ID No. 1489 SEQ ID No. 3465 Listeria monocytogenes 4b Contig594

Corresponding to the former SEQ ID n ° 1770 SEQ ID N ° 3466 Listeria monocytogenes 4b Contig595

Corresponding to the former SEQ ID n ° 1377

Corresponding to the former SEQ ID No. 1689 SEQ ID No. 3467 Listeria monocytogenes 4b Contig596

Corresponding to the former SEQ ID n ° 1225

Corresponding to the former SEQ ID n ° 1759 SEQ ID N ° 3468 Listeria monocytogenes 4b Contig597

SEQ ID N ° 3469 Listeria monocytogenes 4b Contig598 SEQ ID N ° 3470 Listeria monocytogenes 4b Contig599

Corresponding to the former SEQ ID No. 1477 SEQ ID No. 3471 Listeria monocytogenes 4b ContigόOO

Corresponding to the former SEQ ID n ° 1903 SEQ ID N ° 3472 Listeria monocytogenes 4b Contig601

Corresponding to the former SEQ ID No. 1961 SEQ ID No. 3473 Listeria monocytogenes 4b Contig602

Corresponding to the former SEQ ID n ° 1754 SEQ ID N ° 3474 Listeria monocytogenes 4b Contig603

Corresponding to the former SEQ ID n ° 1 188 SEQ ID N ° 3475 Listeria monocytogenes 4b Contig604 SEQ ID N ° 3476 Listeria monocytogenes 4b Contig605

Corresponding to the former SEQ ID n ° 1913 SEQ ID N ° 3477 Listeria monocytogenes 4b Contig606 SEQ ID N ° 3478 Listeria monocytogenes 4b Contig607 SEQ ID N ° 3479 Listeria monocytogenes 4b Contig608

Corresponding to the former SEQ ID n ° 1439

Corresponding to the former SEQ ID n ° 1545 SEQ ID N ° 3480 Listeria monocytogenes 4b Contig609

Corresponding to the former SEQ ID n ° 1794 SEQ ID N ° 3481 Listeria monocytogenes 4b Contigό 10

Corresponding to the former SEQ ID n ° 1798 SEQ ID N ° 3482 Listeria monocytogenes 4b Contigό 1 1

Corresponding to the former SEQ ID n ° 1200 SEQ ID N ° 3483 Listeria monocytogenes 4b Contigό 12

Corresponding to the former SEQ ID n ° 1808 SEQ ID N ° 3484 Listeria monocytogenes 4b Contigό 13

Corresponding to the former SEQ ID n ° 1894 SEQ ID N ° 3485 Listeria monocytogenes 4b Contigό 14

Corresponding to the former SEQ ID n ° 1812 SEQ ID N ° 3486 Listeria monocytogenes 4b Contigό 15

Corresponding to the former SEQ ID n ° 1205 SEQ ID N ° 3487 Listeria monocytogenes 4b Contigό 16 SEQ ID N ° 3488 Listeria monocytogenes 4b Contigό 17 SEQ ID N ° 3489 Listeria monocytogenes 4b Contigό 18

Corresponding to the former SEQ ID n ° 1352 SEQ ID N ° 3490 Listeria monocytogenes 4b Contigό 19

Corresponding to the former SEQ ID No.1142

Corresponding to the former SEQ ID n ° 1601 SEQ ID N ° 3491 Listeria monocytogenes 4b Contig620

Corresponding to the former SEQ ID n ° 1575 SEQ ID N ° 3492 Listeria monocytogenes 4b Contig621

Corresponding to the former SEQ ID n ° 1670 SEQ ID N ° 3493 Listeria monocytogenes 4b Contig622

Corresponding to the former SEQ ID n ° 1890 SEQ ID N ° 3494 Listeria monocytogenes 4b Contig623

Corresponding to the former SEQ ID No. 1333 SEQ ID No. 3495 Listeria monocytogenes 4b Contig624

Corresponding to the former SEQ ID n ° 1789 SEQ ID N ° 3496 Listeria monocytogenes 4b Contig625

Corresponding to the former SEQ ID n ° 1508 SEQ ID N ° 3497 Listeria monocytogenes 4b Contig626 SEQ ID N ° 3498 Listeria monocytogenes 4b Contig627

Corresponding to the former SEQ ID n ° 1775 SEQ ID N ° 3499 Listeria monocytogenes 4b Contig628

Corresponding to the former SEQ ID n ° 1391 SEQ ID N ° 3500 Listeria monocytogenes 4b Contig629

Corresponding to the foπner SEQ ID No. 1657 SEQ ID No. 3501 Listeria monocytogenes 4b Contig630

Corresponding to the former SEQ ID n ° 1851 SEQ ID N ° 3502 Listeria monocytogenes 4b Contig631

SEQ ID N ° 3503 Listeria monocytogenes 4b Contig632

Corresponding to the former SEQ ID n ° 171 1

SEQ ID N ° 3504 Listeria monocytogenes 4b Contig633

Corresponding to the former SEQ ID No.1169

SEQ ID N ° 3505 Listeria monocytogenes 4b Contig634

Corresponding to the former SEQ ID n ° 1660

SEQ ID N ° 3506 Listeria monocytogenes 4b Contig635

SEQ ID N ° 3507 Listeria monocytogenes 4b Contig636

SEQ ID N ° 3508 Listeria monocytogenes 4b Contig637

Corresponding to the former SEQ ID n ° 1767

SEQ ID N ° 3509 Listeria monocytogenes 4b Contig638

SEQ ID N ° 3510 Listeria monocytogenes 4b Contig639

SEQ ID N ° 351 1 Listeria monocytogenes 4b Contig640

Corresponding to the former SEQ ID No. 1992

SEQ ID N ° 3512 Listeria monocytogenes 4b Contig641

Corresponding to the fornier SEQ ID n ° 1413 Corresponding to the former SEQ ID n ° 1515

SEQ ID N ° 3513 Listeria monocytogenes 4b Contig642

Corresponding to the former SEQ ID No. 1 140 Corresponding to the former SEQ ID No. 1373

SEQ ID N ° 3514 Listeria monocytogenes 4b Contig643

Corresponding to the former SEQ ID n ° 1498

SEQ ID N ° 3515 Listeria monocytogenes 4b Contig644

SEQ ID N ° 3516 Listeria monocytogenes 4b Contig645

SEQ ID N ° 3517 Listeria monocytogenes 4b Contig646

Corresponding to the former SEQ ID n ° 1496

SEQ ID N ⁰ 3518 Listeria monocytogenes 4b Contig647 Corresponding to the former SEQ ID n ° 1934 SEQ ID N ⁰ 3519 Listeria monocytogenes 4b Contig648 Corresponding to the former SEQ ID n ° 1650

SEQ ID N ° 3520 Listeria monocytogenes 4b Contig649

Corresponding to the former SEQ ID No. 1233 Corresponding to the former SEQ ID No. 1671

SEQ ID N ⁰ 3521 Listeria monocytogenes 4b Contig650 Corresponding to the former SEQ ID n ° 1950 SEQ ID N ° 3522 Listeria monocytogenes 4b Contig651 Corresponding to the fornier SEQ ID n ° 1889 SEQ ID N ° 3523 Listeria monocytogenes 4b Contig652 Corresponding to the former SEQ ID n ° 1922 SEQ ID N ° 3524 Listeria monocytogenes 4b Contig653 Corresponding to the former SEQ ID n ° 1313 Corresponding to the former SEQ ID n ° 1453

SEQ ID N ° 3525 Listeria monocytogenes 4b Contig654 Corresponding to the former SEQ ID n ° 1 185 SEQ ID N ° 3526 Listeria monocytogenes 4b Contig655 Corresponding to the former SEQ ID n ° 1562 SE _^ Q ^ I .D _^ N,. ° _ 3, -5,, 2_.7, Listeria monocytogenes 4b Contig656

SEQ ID N ° 3528 Listeria monocytogenes 4b Contig657

SEQ ID N ° 3529 Listeria monocytogenes 4b Contig658 SEQ ID N ° 3530 Listeria monocytogenes 4b Contig659 Corresponding to the former SEQ ID n ° 348 SEQ ID N ⁰ 3531 Listeria monocytogenes 4b ContigόόO Corresponding to the former SEQ ID n ° 293 SEQ ID N ° 3532 Listeria monocytogenes 4b Contigόόl Corresponding to the former SEQ ID No. 165 Corresponding to the former SEQ ID No. 762

SEQ ID N ⁰ 3533 Listeria monocytogenes 4b Contig662 Corresponding to the former SEQ ID n ° 640

SEQ ID N ° 3534 Listeria monocytogenes 4b Contig663 SEQ ID N ⁰ 3535 Listeria monocytogenes 4b Contig664 SEQ ID N ⁰ 3536 Listeria monocytogenes 4b Contig665 Corresponding to the former SEQ ID n ° 764

SEQ ID N ° 3537 Listeria monocytogenes 4b Contigόόό Corresponding to the former SEQ ID n ° 543

SEQ ID N ⁰ 3538 Listeria monocytogenes 4b Contig667 Corresponding to the former SEQ ID n ° 844

SEQ ID N ⁰ 3539 Listeria monocytogenes 4b Contig668 Corresponding to the former SEQ ID n ° 560

SEQ ID N ⁰ 3540 Listeria monocytogenes 4b Contig669 Corresponding to the former SEQ ID n ° 744

SEQ ID N ⁰ 3541 Listeria monocytogenes 4b Contig670 Corresponding to the former SEQ ID n ° 796

SEQ ID N ° 3542 Listeria monocytogenes 4b Contig671 Corresponding to the former SEQ ID n ° 776

SEQ ID N ° 3543 Listeria monocytogenes 4b Contig672 Corresponding to the former SEQ ID n ° 897

SEQ ID N ° 3544 Listeria monocytogenes 4b Contig673 Corresponding to the former SEQ ID n ° 704

SEQ ID N ° 3545 Listeria monocytogenes 4b Contig674 Corresponding to the former SEQ ID n ° 713

SEQ ID N ° 3546 Listeria monocytogenes 4b Contig675 Corresponding to the former SEQ ID n ° 295 Corresponding to the former SEQ ID n ° 353

SEQ ID N ° 3547 Listeria monocytogenes 4b Contig676 Corresponding to the former SEQ ID n ° 1303

SEQ ID N ° 3548 Listeria monocytogenes 4b Contig677 SEQ ID N ° 3549 Listeria monocytogenes 4b Contig678 SEQ ID N ° 3550 Listeria monocytogenes 4b Contig679 SEQ ID N ⁰ 3551 Listeria monocytogenes 4b Contig680 SEQ ID N ⁰ 3552 Listeria monocytogenes 4b Contig theb Contig ID No. 1212 Corresponding to the former SEQ ID No. 1521

SEQ ID N ⁰ 3553 Listeria monocytogenes 4b Contig682 SEQ ID N ° 3554 Listeria monocytogenes 4b Contig683 Corresponding to the former SEQ ID n ° 1694

SEQ ID N ° 3555 Listeria monocytogenes 4b Contig684 Corresponding to the former SEQ ID n ° 1939

SEQ ID N ° 3556 Listeria monocytogenes 4b Contig685 Corresponding to the former SEQ ID n ° 1717 SEQ ID N ⁰ 3557 Listeria monocytogenes 4b Contig686 Corresponding to the former SEQ ID n ° 626 SEQ ID N ⁰ 3558 Listeria monocytogenes 4b Contig687 Corresponding to the former SEQ ID n ° 585 SEQ ID N ° 3559 Listeria monocytogenes 4b Contig688 Corresponding to the former SEQ ID No. 491 SEQ ID No. 3560 Listeria monocytogenes 4b Contig689 Corresponding to the former SEQ ID No. 314 Corresponding to the former SEQ ID No. 481

SEQ ID N ⁰ 3561 Listeria monocytogenes 4b Contig690 Corresponding to the former SEQ ID n ° 155 SEQ ID N ° 3562 Listeria monocytogenes 4b Contig691 Corresponding to the former SEQ ID n ° 149 Corresponding to the former SEQ ID n ° 747

SEQ ID N ° 3563 Listeria monocytogenes 4b Contig692 Corresponding to the former SEQ ID n ° 364 SEQ ID N ° 3564 Listeria monocytogenes 4b Contig693 Corresponding to the former SEQ ID n ° 594 SEQ ID N ° 3565 Listeria monocytogenes 4b Contig694 Corresponding to the former SEQ ID No. 398 Corresponding to the former SEQ ID No. 771

SEQ ID N ° 3566 Listeria monocytogenes 4b Contig695 Corresponding to the former SEQ ID n ° 178 Corresponding to the former SEQ ID n ° 684

SEQ ID N ° 3567 Listeria monocytogenes 4b Contig696 Corresponding to the former SEQ ID n ° 433 Corresponding to the former SEQ ID n ° 756

SEQ ID N ° 3568 Listeria monocytogenes 4b Contig697 Corresponding to the former SEQ ID n ° 774

SEQ ID N ° 3569 Listeria monocytogenes 4b Contig698 SEQ ID N ° 3570 Listeria monocytogenes 4b Contig699 Corresponding to the former SEQ ID n ° 300

SEQ ID N ⁰ 3571 Listeria monocytogenes 4b Contig700 SEQ ID N ° 3572 Listeria monocytogenes 4b Contig701 Corresponding to the former SEQ ID n ° 547

SEQ ID N ⁰ 3573 Listeria monocytogenes 4b Contig702 Corresponding to the former SEQ ID n ° 788

SEQ ID N ° 3574 Listeria monocytogenes 4b Contig703 SEQ ID N ⁰ 3575 Listeria monocytogenes 4b Contig704 Corresponding to the former SEQ ID n ° 872

SEQ ID N ⁰ 3576 Listeria monocytogenes 4b Contig705 Corresponding to the former SEQ ID n ° 861

SEQ ID N ° 3577 Listeria monocytogenes 4b Contig706 Corresponding to the former SEQ ID n ° 932

SEQ ID N ⁰ 3578 Listeria monocytogenes 4b Contig707 Corresponding to the former SEQ ID n ° 553

SEQ ID N ⁰ 3579 Listeria monocytogenes 4b Contig708 Corresponding to the former SEQ ID n ° 473

SEQ ID N ⁰ 3580 Listeria monocytogenes 4b Contig709 Corresponding to the former SEQ ID n ° 328 Corresponding to the former SEQ ID n ° 745

SEQ ID N ⁰ 3581 Listeria monocytogenes 4b Contig710 Corresponding to the former SEQ ID n ° 557 SEQ ID N ° 3582 Listeria monocytogenes 4b Contig71 1 Corresponding to the former SEQ ID n ° 773 SEQ ID N ° 3583 Listeria monocytogenes 4b Contig712 Corresponding to the former SEQ ID No. 444 SEQ ID No. 3584 Listeria monocytogenes 4b Contig713 Corresponding to the former SEQ ID No. 826 SEQ ID N ⁰ 3585 Listeria monocytogenes 4b Contig714 Corresponding to the former SEQ ID No. 356 Corresponding to the former SEQ ID No. 612

SEQ ID N ° 3586 Listeria monocytogenes 4b Contig715 Corresponding to the former SEQ ID n ° 952 SEQ ID N ⁰ 3587 Listeria monocytogenes 4b Contig716 Corresponding to the former SEQ ID n ° 874 SEQ ID N ° 3588 Listeria monocytogenes 4b Contig717 Corresponding to the foπner SEQ ID No. 095 Corresponding to the former SEQ ID No. 173

SEQ ID N ⁰ 3589 Listeria monocytogenes 4b Contig718 Corresponding to the former SEQ ID n ° 800 SEQ ID N ° 3590 Listeria monocytogenes 4b Contig719 Corresponding to the foπner SEQ ID n ° 320 Corresponding to the former SEQ ID n ° 832

SEQ ID N ⁰ 3591 Listeria monocytogenes 4b Contig720 Corresponding to the foπner SEQ ID n ° 160 Corresponding to the former SEQ ID n ° 641

SEQ ID N ° 3592 Listeria monocytogenes 4b Contig721 SEQ ID N ° 3593 Listeria monocytogenes 4b Contig722 Corresponding to the former SEQ ID n ° 144 Corresponding to the former SEQ ID n ° 216

SEQ ID N ° 3594 Listeria monocytogenes 4b Contig723 SEQ ID N ° 3595 Listeria monocytogenes 4b Contig724 Corresponding to the former SEQ ID n 2026

SEQ ID N ° 3596 Listeria monocytogenes 4b Contig725 Corresponding to the former SEQ ID n ° 1226 Corresponding to the former SEQ ID n ° 1588

SEQ ID N ⁰ 3597 Listeria monocytogenes 4b Contig726 Corresponding to the former SEQ ID n ° 1804 SEQ ID N ° 3598 Listeria monocytogenes 4b Contig727 Corresponding to the former SEQ ID n 1393 SEQ ID N ° 3599 Listeria monocytogenes 4b Contig728 Corresponding to the fornier SEQ ID n 223 SEQ ID N ° 3600 Listeria monocytogenes 4b Contig729 Corresponding to the former SEQ ID n ° 1973 SEQ ID N ⁰ 3601 Listeria monocytogenes 4b Contig730 Corresponding to the former SEQ ID n ° 1743 SEQ ID N ° 3602 Listeria monocytogenes 4b Contig731 Corresponding to the former SEQ ID No. 1860 SEQ ID No. 3603 Listeria monocytogenes 4b Contig732 Corresponding to the foπner SEQ ID n ° 1203

SEQ ID N ° 3604 Listeria monocytogenes 4b Contig733 Corresponding to the foπner SEQ ID n ° 1690 Corresponding to the former SEQ ID n ° 1701

SEQ ID N ° 3605 Listeria monocytogenes 4b Contig734 Corresponding to the former SEQ ID n ° 1525

SEQ ID N ° 3606 Listeria monocytogenes 4b Contig735 Corresponding to the former SEQ ID n ° 1272

SEQ ID N ° 3607 Listeria monocytogenes 4b Contig736 SEQ ID N ° 3608 Listeria monocytogenes 4b Contig737 Corresponding to the former SEQ ID n ° 1986

SEQ ID N ° 3609 Listeria monocytogenes 4b Contig738 Corresponding to the former SEQ ID n ° 1799

SEQ ID N ⁰ 3610 Listeria monocytogenes 4b Contig739 SEQ ID N ⁰ 361 1 Listeria monocytogenes 4b Contig740 Corresponding to the former SEQ ID n ° 1070 Corresponding to the foπner SEQ ID n ° 1783

SEQ ID N ⁰ 3612 Listeria monocytogenes 4b Contig741 SEQ ID N ⁰ 3613 Listeria monocytogenes 4b Contig742 SEQ ID N ⁰ 3614 Listeria monocytogenes 4b Contig743 Corresponding to the former SEQ ID n ° 1437

SEQ ID N ⁰ 3615 Listeria monocytogenes 4b Contig744 Corresponding to the former SEQ ID n ° 1094 Corresponding to the former SEQ ID n ° 1523

SEQ ID N ⁰ 3616 Listeria monocytogenes 4b Contig745 Corresponding to the former SEQ ID n ° 1929 SEQ ID N ⁰ 3617 Listeria monocytogenes 4b Contig746 Corresponding to the former SEQ ID n ° 1383 Corresponding to the former SEQ ID n ° 1486

SEQ ID N ⁰ 3618 Listeria monocytogenes 4b Contig747 SEQ ID N ⁰ 3619 Listeria monocytogenes 4b Contig748 Corresponding to the foπner SEQ ID n ° 1957

SEQ ID N ° 3620 Listeria monocytogenes 4b Contig749 SEQ ID N ⁰ 3621 Listeria monocytogenes 4b Contig750 Corresponding to the former SEQ ID n ° 1859 Corresponding to the former SEQ ID n ° 1963

SEQ ID N ° 3622 Listeria monocytogenes 4b Contig751 SEQ ID N ° 3623 Listeria monocytogenes 4b Contig752 SEQ ID N ° 3624 Listeria monocytogenes 4b Contig753 Corresponding to the former SEQ ID n ° 1971

SEQ ID N ° 3625 Listeria monocytogenes 4b Contig754 Corresponding to the former SEQ ID n ° 1 189 Corresponding to the former SEQ ID n ° 1289 Corresponding to the former SEQ ID n ° 1619

SEQ ID N ° 3626 Listeria monocytogenes 4b Contig755 SEQ ID N ° 3627 Listeria monocytogenes 4b Contig756 Corresponding to the former SEQ ID n ° 1883

SEQ ID N ° 3628 Listeria monocytogenes 4b Contig757 Corresponding to the former SEQ ID n ° 1316 Corresponding to the foπner SEQ ID n ° 1460 SEQ ID N ° 3629 Listeria monocytogenes 4b Contig758 SEQ ID N ⁰ 3630 Listeria monocytogenes 4b Contig759 Corresponding to the former SEQ ID n ° 1389

SEQ ID N ⁰ 3631 Listeria monocytogenes 4b Contig760 SEQ ID N ° 3632 Listeria monocytogenes 4b Contig761 Corresponding to the former SEQ ID n ° 1397

SEQ ID N ° 3633 Listeria monocytogenes 4b Contig762 Corresponding to the former SEQ ID n ° 1261 Corresponding to the former SEQ ID n ° 1531

SEQ ID N ° 3634 Listeria monocytogenes 4b Contig763 Corresponding to the former SEQ ID n ° 1563 SEQ ID N ° 3635 Listeria monocytogenes 4b Contig764 Corresponding to the former SEQ ID n ° 1945 SEQ ID N ⁰ 3636 Listeria monocytogenes 4b Contig765 Corresponding to the former SEQ ID n ° 1306 SEQ ID N ° 3637 Listeria monocytogenes 4b Contig766 Corresponding to the former SEQ ID n ° 1253 SEQ ID N ° 3638 Listeria monocytogenes 4b Contig767 Corresponding to the former SEQ ID n ° 1 1 16 Corresponding to the former SEQ ID n ° 1,154

SEQ ID N ° 3639 Listeria monocytogenes 4b Contig768 Corresponding to the former SEQ ID n ° 1807 SEQ ID N ° 3640 Listeria monocytogenes 4b Contig769 Corresponding to the former SEQ ID n ° 1580 SEQ ID N ⁰ 3641 Listeria monocytogenes 4b Contig770 Corresponding to the former SEQ ID No. 1234 Corresponding to the foπner SEQ ID No. 1951

SEQ ID N ° 3642 Listeria monocytogenes 4b Contig771 Corresponding to the former SEQ ID n ° 1933 SEQ ID N ° 3643 Listeria monocytogenes 4b Contig772 Corresponding to the former SEQ ID n ° 1 186 Corresponding to the former SEQ ID n ° 1462

SEQ ID N ° 3644 Listeria monocytogenes 4b Contig773 Corresponding to the former SEQ ID n ° 1073 Corresponding to the former SEQ ID n ° 1 167 Corresponding to the former SEQ ID n ° 1322

SEQ ID N ° 3645 Listeria monocytogenes 4b Contig774 Corresponding to the former SEQ ID n ° 1686 SEQ ID N ° 3646 Listeria monocytogenes 4b Contig775 Corresponding to the former SEQ ID n ° 2005 SEQ ID N ° 3647 Listeria monocytogenes 4b Contig776 Corresponding to the foπner SEQ ID No. 1385 Corresponding to the former SEQ ID No. 1685

SEQ ID N ° 3648 Listeria monocytogenes 4b Contig777 Corresponding to the foπner SEQ ID n ° 1277 Corresponding to the former SEQ ID n ° 1785

SEQ ID N ° 3649 Listeria monocytogenes 4b Contig778 SEQ ID N ⁰ 3650 Listeria monocytogenes 4b Contig779 Corresponding to the former SEQ ID n ° 1946

SEQ ID N ⁰ 3651 Listeria monocytogenes 4b Contig780 Corresponding to the former SEQ ID No. 388 Corresponding to the foπner SEQ ID No. 732

SEQ ID N ° 3652 Listeria monocytogenes 4b Contig781 Corresponding to the former SEQ ID n ° 652 Corresponding to the former SEQ ID n ° 697

SEQ ID N ° 3653 Listeria monocytogenes 4b Contig782 Corresponding to the former SEQ ID n ° 244 Corresponding to the former SEQ ID n ° 286

SEQ ID N ° 3654 Listeria monocytogenes 4b Contig783 Corresponding to the former SEQ ID n ° 275 Corresponding to the former SEQ ID n ° 421

SEQ ID No. 3655 Listeria monocytogenes 4b Contig784 Corresponding to the former SEQ ID No. 240 SEQ ID No. 3656 Listeria monocytogenes 4b Contig785 Corresponding to the former SEQ ID No. 129 Corresponding to the former SEQ ID No. 384 Corresponding to the former SEQ ID # 469

SEQ ID N ° 3657 Listeria monocytogenes 4b Contig786 Corresponding to the former SEQ ID n ° 610 Corresponding to the former SEQ ID n ° 857

SEQ ID No. 3658 Listeria monocytogenes 4b Contig787 Corresponding to the former SEQ ID No. 484 SEQ ID No. 3659 Listeria monocytogenes 4b Contig788 Corresponding to the former SEQ ID No. 081 Corresponding to the former SEQ ID No. 117 Corresponding to the former SEQ ID # 196

SEQ ID N ° 3660 Listeria monocytogenes 4b Contig789 Corresponding to the former SEQ ID n ° 175 SEQ ID N ⁰ 3661 Listeria monocytogenes 4b Contig790 Corresponding to the former SEQ ID n ° 727 SEQ ID N ° 3662 Listeria monocytogenes 4b Contig791 Corresponding to the former SEQ ID n ° 925 SEQ ID N ° 3663 Listeria monocytogenes 4b Contig792 Corresponding to the former SEQ ID n ° 134 Corresponding to the former SEQ ID n ° 157 Corresponding to the tonner SEQ ID n ° 779

SEQ ID N ° 3664 Listeria monocytogenes 4b Contig793 Corresponding to the former SEQ ID n ° 795

SEQ ID N ° 3665 Listeria monocytogenes 4b Contig794 Corresponding to the former SEQ ID n ° 988

SEQ ID N ° 3666 Listeria monocytogenes 4b Contig795 Corresponding to the foπner SEQ ID n ° 616

SEQ ID N ° 3667 Listeria monocytogenes 4b Contig796 SEQ ID N ° 3668 Listeria monocytogenes 4b Contig797 Corresponding to the former SEQ ID n ° 103

SEQ ID N ° 3669 Listeria monocytogenes 4b Contig798 Corresponding to the former SEQ ID n ° 51 1

SEQ ID N ° 3670 Listeria monocytogenes 4b Contig799 Corresponding to the former SEQ ID n ° 446

SEQ ID N ⁰ 3671 Listeria monocytogenes 4b Contig800 Conesponding to the former SEQ ID n ° 1264

Corresponding to the former SEQ ID No. 2017 SEQ ID No. 3672 Listeria monocytogenes 4b Contig801

Corresponding to the former SEQ ID No.1151

Corresponding to the former SEQ ID n ° 1577 SEQ ID N ° 3673 Listeria monocytogenes 4b Contig802

Corresponding to the former SEQ ID n ° 1520

Corresponding to the former SEQ ID n ° 1755 SEQ ID N ° 3674 Listeria monocytogenes 4b Contig803

Corresponding to the foπner SEQ ID n ° 1291

Corresponding to the former SEQ ID n ° 1305

Corresponding to the foπner SEQ ID No. 1589 SEQ ID No. 3675 Listeria monocytogenes 4b Contig804

Corresponding to the former SEQ ID n ° 1207 SEQ ID N ° 3676 Listeria monocytogenes 4b Contig805

Conesponding to the former SEQ ID No. 1987 SEQ ID No. 3677 Listeria monocytogenes 4b Contig806

Conesponding to the former SEQ ID n ° 1912 SEQ ID N ° 3678 Listeria monocytogenes 4b Contig807

Corresponding to the former SEQ ID n ° 1432

Corresponding to the former SEQ ÏD n ° 1681 SEQ ID N ° 3679 Listeria monocytogenes 4b Contig808

Corresponding to the former SEQ ID n ° 1955 SEQ ID N ° 3680 Listeria monocytogenes 4b Contig809

Corresponding to the former SEQ ID n ° 1382 SEQ ID N ° 3681 Listeria monocytogenes 4b Contigδ 10

Corresponding to the former SEQ ID n ° 1885 SEQ ID N ° 3682 Listeria monocytogenes 4b Contig81 1

SEQ ID N ° 3683 Listeria monocytogenes 4b Contig812

Corresponding to the former SEQ ID n ° 1451

Corresponding to the former SEQ ID n ° 1592 SEQ ID N ° 3684 Listeria monocytogenes 4b Contigδ 13

Corresponding to the foπner SEQ ID n ° 1402

Corresponding to the former SEQ ID n ° 1647

Corresponding to the former SEQ ID n ° 1768 SEQ ID N ° 3685 Listeria monocytogenes 4b Contig814

Corresponding to the former SEQ ID n ° 1522 SEQ ID N ° 3686 Listeria monocytogenes 4b Contig815

Corresponding to the former SEQ ID No. 1984 SEQ ID No. 3687 Listeria monocytogenes 4b Contigδ 16

Corresponding to the foπner SEQ ID No. 1335 SEQ ID No. 3688 Listeria monocytogenes 4b Contig817

Corresponding to the former SEQ ID n ° 2007 SEQ ID N ° 3689 Listeria monocytogenes 4b Contig818

Corresponding to the former SEQ ID n ° 1 121 SEQ ID N ° 3690 Listeria monocytogenes 4b Contigδ 19

Corresponding to the former SEQ ID n ° 1 182 SEQ ID N ° 3691 Listeria monocytogenes 4b Contig820

Corresponding to the former SEQ ID n ° 1554 SEQ ID N ° 3692 Listeria monocytogenes 4b Contig821 Conesponding to the former SEQ ID n ° 1 153 Conesponding to the former SEQ ID n ° 1458

SEQ ID N ° 3693 Listeria monocytogenes 4b Contig822 Conesponding to the former SEQ ID n ° 254 Corresponding to the former SEQ ID n ° 864 SEQ ID N ° 3694 Listeria monocytogenes 4b Contig823 Conesponding to the former SEQ ID n ° 407 Corresponding to the former SEQ ID n ° 736 SEQ ID N ° 3695 Listeria monocytogenes 4b Contig824 Conesponding to the former SEQ ID n ° 412 Corresponding to the former SEQ ID n ° 482 SEQ ID N ° 3696 Listeria monocytogenes 4b Contig825 Conesponding to the former SEQ ID n ° 360 Conesponding to the former SEQ ID no.375 Conesponding to the former SEQ ID no.645

SEQ ID N ° 3697 Listeria monocytogenes 4b Contig826 Corresponding to the former SEQ ID n ° 164 SEQ ID N ° 3698 Listeria monocytogenes 4b Contig827 Corresponding to the former SEQ ID n ° 459 Corresponding to the former SEQ ID n ° 772

SEQ ID N ° 3699 Listeria monocytogenes 4b Contig828 Corresponding to the former SEQ ID n ° 334 Corresponding to the foπner SEQ ID n ° 533 Conesponding to the foπner SEQ ID n ° 750

SEQ ID N ° 3700 Listeria monocytogenes 4b Contig829 Corresponding to the former SEQ ID n ° 586 SEQ ID N ⁰ 3701 Listeria monocytogenes 4b Contig830 Corresponding to the former SEQ ID n ° 2021 SEQ ID N ° 3702 Listeria monocytogenes 4b Contig831 Corresponding to the former SEQ ID No. 374 Corresponding to the former SEQ ID No. 819

SEQ ID N ° 3703 Listeria monocytogenes 4b Contig832 Corresponding to the former SEQ ID n ° 425 Conesponding to the former SEQ ID n ° 598

SEQ ID N ° 3704 Listeria monocytogenes 4b Contig833 Corresponding to the foπner SEQ ID n ° 198 Corresponding to the former SEQ ID n ° 296

SEQ ID N ° 3705 Listeria monocytogenes 4b Contig834 Conesponding to the former SEQ ID n ° 893 SEQ ID N ° 3706 Listeria monocytogenes 4b Contig835 Corresponding to the former SEQ ID n ° 1 19 Corresponding to the former SEQ ID n ° 720

SEQ ID N ° 3707 Listeria monocytogenes 4b Contig836 Corresponding to the former SEQ ID n ° 457 SEQ ID N ° 3708 Listeria monocytogenes 4b Contig837 Corresponding to the former SEQ ID n ° 655 Corresponding to the former SEQ ID n ° 880

SEQ ID N ° 3709 Listeria monocytogenes 4b Contig838 Corresponding to the former SEQ ID n ° 1 19 SEQ ID ⁰ 3710 Listeria monocytogenes 4b Contig839 oo r ^ NO o _. CN CN or ^ Tf NO o CN Tf CN CN m ON 00 ON ON oo oo 00 00 NO o 00 f C sr> oo ON sn m T ON s CN SD sr> NO 00 00 r- Tf o CN sn CN oo ON NO ON <N m CN r ^ r ^ r ^ m - 'Tt "00 - ™" ~ CN Tt "-' ON ON NO 00 Tf 00 - T" * - 'sn CN r ^ CN ON ON O m oo CN NO

90 90 DQQQQ Û Û QQQ o D g QQQQ o O α OO α O o OOO α OO o O w0 w CΛ w 00 wwww ω wm P ω LU WWPP o 0 00 00 00 00 00 om 00 CΛ 00 CΛ 00 CΛ CΛ

Conesponding to the former SEQ ID n ° 1705

SEQ ID N ⁰ 3728 Listeria monocytogenes 4b Contig857 Corresponding to the former SEQ ID n ° 1479 Corresponding to the former SEQ ID n ° 1972

SEQ ID N ° 3729 Listeria monocytogenes 4b Contig858 Corresponding to the former SEQ ID n ° 1307 Corresponding to the foπner SEQ ID n ° 1829

SEQ ID N ° 3730 Listeria monocytogenes 4b Contig859 Corresponding to the foπner SEQ ID n ° 1 123 Corresponding to the former SEQ ID n ° 1687

SEQ ID N ⁰ 3731 Listeria monocytogenes 4b Contig860 Corresponding to the former SEQ ID n ° 1905 SEQ ID N ° 3732 Listeria monocytogenes 4b Contig861 Corresponding to the former SEQ ID n ° 1579 SEQ ID N ° 3733 Listeria monocytogenes 4b Contig862 Corresponding to the foπner SEQ ID No. 1080 Corresponding to the former SEQ ID No. 1146

SEQ ID N ⁰ 3734 Listeria monocytogenes 4b Contig863 Corresponding to the former SEQ ID n ° 1 1 1 1 Corresponding to the former SEQ ID n ° 1514

SEQ ID N ⁰ 3735 Listeria monocytogenes 4b Contig864 Corresponding to the former SEQ ID n ° 1 139 Corresponding to the former SEQ ID n ° 1602

SEQ ID N ° 3736 Listeria monocytogenes 4b Contig865 Corresponding to the former SEQ ID n ° 1221 Corresponding to the former SEQ ID n ° 2010

SEQ ID N ° 3737 Listeria monocytogenes 4b Contig866 Corresponding to the foπner SEQ ID n ° 1 174 Corresponding to the former SEQ ID n ° 1480 Corresponding to the former SEQ ID n ° 1895

SEQ ID N ⁰ 3738 Listeria monocytogenes 4b Contig867 Corresponding to the former SEQ ID n ° 1780 Corresponding to the foπner SEQ ID n ° 1784

SEQ ID N ⁰ 3739 Listeria monocytogenes 4b Contig868 Corresponding to the former SEQ ID n ° 2009 SEQ ID N ° 3740 Listeria monocytogenes 4b Contig869 Conesponding to the former SEQ ID n ° 1308 Conesponding to the former SEQ ID n ° 1597

SEQ ID N ⁰ 3741 Listeria monocytogenes 4b Contig870 Corresponding to the former SEQ ID n ° 131 1 Conesponding to the former SEQ ID n ° 1315 Conesponding to the foπner SEQ ID n ° 2003

SEQ ID N ° 3742 Listeria monocytogenes 4b Contig871 Corresponding to the former SEQ ID n ° 1493 Corresponding to the foπner SEQ ID n ° 1707 SEQ ID N ° 3743 Listeria monocytogenes 4b Contig872 Corresponding to the former SEQ ID n ° 1089 Corresponding to the foπner SEQ ID n ° 1624 SEQ ID N ° 3744 Listeria monocytogenes 4b Contig873 Corresponding to the former SEQ ID n ° 1846 SEQ ID N ° 3745 Listeria monocytogenes 4b Contig874 Conesponding to the foπner SEQ ID n ° 603 Conesponding to the former SEQ ID n ° 921 SEQ ID N ° 3746 Listeria monocytogenes 4b Contig875 Corresponding to the former SEQ ID n ° 268 Conesponding to the former SEQ ID n ° 752 SEQ ID N ° 3747 Listeria monocytogenes 4b Contig876 Conesponding to the former SEQ ID n ° 336 Conesponding to the former SEQ ID n ° 623 SEQ ID N ° 3748 Listeria monocytogenes 4b Contig877 Conesponding to the former SEQ ID n ° 259 Conesponding to the former SEQ ID No. 551 Corresponding to the former SEQ ID No. 866

SEQ ID N ° 3749 Listeria monocytogenes 4b Contig878 Conesponding to the foπner SEQ ID n ° 224 SEQ ID N ° 3750 Listeria monocytogenes 4b Contig879 Conesponding to the former SEQ ID n ° 418 Conesponding to the former SEQ ID n ° 571 Conesponding to the former SEQ ID # 809

SEQ ID N ⁰ 3751 Listeria monocytogenes 4b Contig880 Corresponding to the former SEQ ID n ° 420 Conesponding to the former SEQ ID n ° 664 SEQ ÏD N ° 3752 Listeria monocytogenes 4b Contig881 Corresponding to the former SEQ ID n ° 137 Corresponding to the former SEQ ID n ° 367 SEQ ID N ° 3753 Listeria monocytogenes 4b Contig882 Conesponding to the foπner SEQ ID n ° 222 Conesponding to the former SEQ ID n ° 318 Conesponding to the former SEQ ID n ° 758

SEQ ID No. 3754 Listeria monocytogenes 4b Contig883 Corresponding to the former SEQ ID No. 978 SEQ ID No. 3755 Listeria monocytogenes 4b Contig884 Conesponding to the foπner SEQ ID No. 793 Corresponding to the former SEQ ID No. 855

SEQ ID N ° 3756 Listeria monocytogenes 4b Contig885 Conesponding to the foπner SEQ ID n ° 236 Conesponding to the former SEQ ID n ° 666 Conesponding to the former SEQ ID n ° 892

SEQ ID ⁰ 3757 Listeria monocytogenes 4b Contig886 Conesponding to the former SEQ ID n ° 466 Conesponding to the former SEQ ID n ° 825

SEQ ID N ⁰ 3758 Listeria monocytogenes 4b Contig887 Conesponding to the foπner SEQ ID n ° 901 SEQ ID n ° 3759 Listeria monocytogenes 4b Contig888 Conesponding to the foπner SEQ ID n ° 761 Conesponding to the former SEQ ID n ° 947

SEQ ID N ° 3760 Listeria monocytogenes 4b Contig889 Corresponding to the foπner SEQ ID n ° 517 Corresponding to the former SEQ ID n ° 943

SEQ ID N ⁰ 3761 Listeria monocytogenes 4b Contig890 Conesponding to the former SEQ ID n ° 654 Corresponding to the former SEQ ID n ° 787

SEQ ID N ⁰ 3762 Listeria monocytogenes 4b Contig891 Conesponding to the former SEQ ID n ° 427 Conesponding to the foπner SEQ ID n ° 2019

SEQ ID N ⁰ 3763 Listeria monocytogenes 4b Contig892 Conesponding to the former SEQ ID n ° 441 Conesponding to the foπner SEQ ID n ° 974

SEQ ID N ° 3764 Listeria monocytogenes 4b Contig893 Conesponding to the former SEQ ID n ° 411 Conesponding to the fonner SEQ ID n ° 733

SEQ ID N ° 3765 Listeria monocytogenes 4b Contig894 SEQ ID N ° 3766 Listeria monocytogenes 4b Contig895 Conesponding to the fonner SEQ ID n ° 994

SEQ ID N ° 3767 Listeria monocytogenes 4b Contig896 Conesponding to the foπner SEQ ID n ° 552

SEQ ID N ° 3768 Listeria monocytogenes 4b Contig897 Conesponding to the former SEQ ID n ° 442

SEQ ID N ° 3769 Listeria monocytogenes 4b Contig898 Conesponding to the former SEQ ID n ° 150 Conesponding to the former SEQ ID n ° 937 Corresponding to the foπner SEQ ID n ° 980

SEQ ID N ° 3770 Listeria monocytogenes 4b Contig899 Conesponding to the former SEQ ID n ° 258 Conesponding to the former SEQ ID n ° 816

SEQ ID N ⁰ 3771 Listeria monocytogenes 4b Contig900 Conesponding to the former SEQ ID n ° 983 SEQ ID N ° 3772 Listeria monocytogenes 4b Contig901 Conesponding to the former SEQ ID n ° 422 Corresponding to the foπner SEQ ID n ° 726

SEQ ID N ⁰ 3773 Listeria monocytogenes 4b Contig902 Conesponding to the former SEQ ÏD n ° 410 Conesponding to the former SEQ ID n ° 778

SEQ ID N ° 3774 Listeria monocytogenes 4b Contig903 Conesponding to the former SEQ ID n ° 232 Conesponding to the former SEQ ID n ° 487

SEQ ID N ° 3775 Listeria monocytogenes 4b Contig904 Conesponding to the former SEQ ID n ° 898 SEQ ID N ° 3776 Listeria monocytogenes 4b Contig905 Conesponding to the foπner SEQ ID n ° 368 Conesponding to the former SEQ ID n ° 985

SEQ ID N ° 3777 Listeria monocytogenes 4b Contig906 Conesponding to the foπner SEQ ID n ° 997 SEQ ID N ⁰ 3778 Listeria monocytogenes 4b Contig907 Corresponding to the foπner SEQ ID n ° 321 Corresponding to the foπner SEQ ID n ° 542 Conesponding to the former SEQ ID # 843

SEQ ID N ° 3779 Listeria monocytogenes 4b Contig908 Corresponding to the foπner SEQ ID n ° 530 Corresponding to the former SEQ ID n ° 667 SEQ ID N ° 3780 Listeria monocytogenes 4b Contig909

Corresponding to the former SEQ ID No. 1534 Corresponding to the former SEQ ID No. 1936

SEQ ID N ° 3781 Listeria monocytogenes 4b Contig910

Corresponding to the former SEQ ID No. 1567 Conesponding to the former SEQ ID No. 1587 Corresponding to the former SEQ ID No. 1642 Corresponding to the former SEQ ID No. 1674

SEQ ID N ° 3782 Listeria monocytogenes 4b Contig91 1

Corresponding to the fonner SEQ ID No. 2000

SEQ ID N ° 3783 Listeria monocytogenes 4b Contig912

Corresponding to the foπner SEQ ID n ° 1909

SEQ ID N ° 3784 Listeria monocytogenes 4b Contig913

Corresponding to the former SEQ ID No. 1 106 Corresponding to the foπner SEQ ID No. 1365 Corresponding to the former SEQ ID No. 1734

SEQ ID N ° 3785 Listeria monocytogenes 4b Contig914

Corresponding to the foπner SEQ ID No. 1284 Corresponding to the former SEQ ID No. 1309 Corresponding to the former SEQ ID No. 1613

SEQ ID N ° 3786 Listeria monocytogenes 4b Contig915

Corresponding to the former SEQ ID No. 2002

SEQ ID N ° 3787 Listeria monocytogenes 4b Contig916

Corresponding to the foπner SEQ ID n ° 1271 Corresponding to the former SEQ ID n ° 1941

SEQ ID N ° 3788 Listeria monocytogenes 4b Contig917

Corresponding to the foπner SEQ ID No. 1 104 Corresponding to the former SEQ ID No. 2030

SEQ ID N ° 3789 Listeria monocytogenes 4b Contig918

Corresponding to the former SEQ ID No. 1959

SEQ ID N ° 3790 Listeria monocytogenes 4b Contig919

Corresponding to the foπner SEQ ID No. 1266 Corresponding to the foπner SEQ ID No. 2020

SEQ ID N ° 3791 Listeria monocytogenes 4b Contig920

Corresponding to the former SEQ ID No. 1405 Corresponding to the foπner SEQ ID No. 1718 Corresponding to the former SEQ ID No. 1919

SEQ ID N ° 3792 Listeria monocytogenes 4b Contig921

Corresponding to the foπner SEQ ID n ° 1908

SEQ ID N ° 3793 Listeria monocytogenes 4b Contig922

Corresponding to the former SEQ ID n ° 1786

SEQ ID N ° 3794 Listeria monocytogenes 4b Contig923

Corresponding to the former SEQ ID No. 1370 Corresponding to the foπner SEQ ID No. 1371 Corresponding to the foπner SEQ ID No. 1372 Corresponding to the former SEQ ID No. 1574

SEQ ID N ° 3795 Listeria monocytogenes 4b Contig924

Corresponding to the former SEQ ID n ° 1488

SEQ ID N ° 3796 Listeria monocytogenes 4b Contig925

Corresponding to the former SEQ ID n ° 1532 Conesponding to the fonner SEQ ID No 2008

SEQ ID N ° 3797 Listeria monocytogenes 4b Contig926 Corresponding to the former SEQ ID n ° 677 Corresponding to the former SEQ ID n ° 906

SEQ ID N ° 3798 Listeria monocytogenes 4b Contig927 Corresponding to the foπner SEQ ID n ° 497 Corresponding to the former SEQ ID n ° 699 Corresponding to the former SEQ ID n ° 700 Corresponding to the former SEQ ID n ° 948

SEQ ID N ° 3799 Listeria monocytogenes 4b Contig928 Corresponding to the former SEQ ID n ° 891 SEQ ID N ° 3800 Listeria monocytogenes 4b Contig929 Corresponding to the former SEQ ID n ° 633 Corresponding to the former SEQ ID n ° 656

SEQ ID N ⁰ 3801 Listeria monocytogenes 4b Contig930 Corresponding to the former SEQ ID n ° 419 Corresponding to the former SEQ ID n ° 494

SEQ ID N ⁰ 3802 Listeria monocytogenes 4b Contig931 Corresponding to the former SEQ ID n ° 2027 SEQ ID N ° 3803 Listeria monocytogenes 4b Contig932 Corresponding to the former SEQ ID n ° 814 Corresponding to the former SEQ ID n ° 828

SEQ ID No. 3804 Listeria monocytogenes 4b Contig933 Corresponding to the former SEQ ID No. 400 Corresponding to the former SEQ ID No. 628 Corresponding to the former SEQ ID No. 698 SEQ ID No. 3805 Listeria monocytogenes 4b Contig934 Corresponding to the former SEQ ID No. 513 Corresponding to the former SEQ ID No. 695 Corresponding to the former SEQ ID No. 960

SEQ ID N ° 3806 Listeria monocytogenes 4b Contig935 Corresponding to the former SEQ ID n ° 648 Corresponding to the former SEQ ID n ° 2018

SEQ ID N ° 3807 Listeria monocytogenes 4b Contig936 Corresponding to the former SEQ ID n ° 238 Corresponding to the former SEQ ID n ° 636

SEQ ID N ° 3808 Listeria monocytogenes 4b Contig937 SEQ ID N ° 3809 Listeria monocytogenes 4b Contig938 Corresponding to the former SEQ ID n ° 341 Corresponding to the former SEQ ID n ° 836 Corresponding to the foπner SEQ ID n ° 848

SEQ ID N ⁰ 3810 Listeria monocytogenes 4b Contig939 Corresponding to the former SEQ ID n ° 087 Corresponding to the former SEQ ID n ° 381 SEQ ID N ⁰ 381 1 Listeria monocytogenes 4b Contig940 Corresponding to the former SEQ ID n ° 288 Corresponding to the foπner SEQ ID No. 386 Corresponding to the former SEQ ID No. 881

SEQ ID N ⁰ 3812 Listeria monocytogenes 4b Contig941 Corresponding to the former SEQ ID n ° 729 Conesponding to the former SEQ ID n ° 2014

SEQ ID N ⁰ 3813 Listeria monocytogenes 4b Contig942 Corresponding to the former SEQ ID n ° 1319 Conesponding to the former SEQ ID n ° 1470 Conesponding to the foπner SEQ ID n ° 1904

SEQ ID N ⁰ 3814 Listeria monocytogenes 4b Contig943 Conesponding to the former SEQ ID n ° 1447 Conesponding to the former SEQ ID n ° 1810

SEQ ID N ⁰ 3815 Listeria monocytogenes 4b Contig944 Corresponding to the former SEQ ID n ° 1999 SEQ ID N ⁰ 3816 Listeria monocytogenes 4b Contig945 Corresponding to the former SEQ ID n ° 1 127 Corresponding to the former SEQ ID n ° 1504 Corresponding to the former SEQ ID No. 1507 Corresponding to the former SEQ ID No. 1631

SEQ ID N ⁰ 3817 Listeria monocytogenes 4b Contig946 Corresponding to the former SEQ ID n ° 201 1 SEQ ID N ⁰ 3818 Listeria monocytogenes 4b Contig947 Corresponding to the former SEQ ID n ° 1475 Conesponding to the former SEQ ID n ° 161 1 Conesponding to the form SEQ ID n ° 1672

SEQ ID N ⁰ 3819 Listeria monocytogenes 4b Contig948 Conesponding to the former SEQ ID n ° 1088 Conesponding to the former SEQ ID n ° 1539 SEQ ID N ° 3820 Listeria monocytogenes 4b Contig949 Corresponding to the former SEQ ID n ° 1204 Corresponding to the former SEQ ID No. 1347 Corresponding to the foπner SEQ ID No. 1845

SEQ ID N ⁰ 3821 Listeria monocytogenes 4b Contig950 Conesponding to the former SEQ ID n ° 1706 Corresponding to the former SEQ ID n ° 1869 Corresponding to the former SEQ ID n ° 1976

SEQ ID N ⁰ 3822 Listeria monocytogenes 4b Contig951 Corresponding to the former SEQ ID n ° 1620 SEQ ID N ° 3823 Listeria monocytogenes 4b Contig952 Corresponding to the former SEQ ID n ° 1886 Corresponding to the former SEQ ID n ° 1935

SEQ ID No. 3824 Listeria monocytogenes 4b Contig953 Corresponding to the former SEQ ID No. 1279 Corresponding to the foπner SEQ ID No. 1301 Corresponding to the former SEQ ID No. 1827

SEQ ID N ⁰ 3825 Listeria monocytogenes 4b Contig954 Corresponding to the foπner SEQ ID n ° 1605 Conesponding to the former SEQ ID n ° 1753 Conesponding to the former SEQ ID n ° 1792

SEQ ID N ° 3826 Listeria monocytogenes 4b Contig955 Corresponding to the former SEQ ID n ° 1998 SEQ ID N ° 3827 Listeria monocytogenes 4b Contig956 Corresponding to the former SEQ ID n ° 1310 Corresponding to the former SEQ ID n ° 1632 Corresponding to the former SEQ ID n ° 1853

SEQ ID N ° 3828 Listeria monocytogenes 4b Contig957 Corresponding to the former SEQ ID n ° 1914 Corresponding to the former SEQ ID n ° 1968 SEQ ID N ° 3829 Listeria monocytogenes 4b Contig958 Corresponding to the foπner SEQ ÏD n ° 1569 Corresponding to the former SEQ ID n ° 1801 SEQ ID N ° 3830 Listeria monocytogenes 4b Contig959 Corresponding to the former SEQ ID n ° 1369 Corresponding to the former SEQ ID n ° 1931 SEQ ID N ⁰ 3831 Listeria monocytogenes 4b Contig960 Corresponding to the former SEQ ID n ° 1247 Corresponding to the former SEQ ID No. 1617 Corresponding to the former SEQ ID No. 1731

SEQ ID N ° 3832 Listeria monocytogenes 4b Contig961 Conesponding to the former SEQ ID n ° 1302 Corresponding to the former SEQ ID n ° 1920 Corresponding to the former SEQ ID n ° 2012

SEQ ID N ⁰ 3833 Listeria monocytogenes 4b Contig962 Corresponding to the former SEQ ID n ° 1068 Corresponding to the former SEQ ID n ° 1072 Corresponding to the former SEQ ID n ° 1635

SEQ ID N ° 3834 Listeria monocytogenes 4b Contig963 Corresponding to the former SEQ ID n ° 1757 Corresponding to the former SEQ ID n ° 2024 SEQ ID N ° 3835 Listeria monocytogenes 4b Contig964 Corresponding to the former SEQ ID n ° 1509 Corresponding to the fonner SEQ ID n ° 1831 SEQ ID N ° 3836 Listeria monocytogenes 4b Contig965 Corresponding to the former SEQ ID n ° 1097 Corresponding to the former SEQ ID n ° 1230 Corresponding to the former SEQ ID n ° 1760

SEQ ID N ° 3837 Listeria monocytogenes 4b Contig966 Corresponding to the former SEQ ID n ° 1343 Corresponding to the former SEQ ID n ° 1766 Corresponding to the former SEQ ID n ° 1878

SEQ ID N ⁰ 3838 Listeria monocytogenes 4b Contig967 Corresponding to the former SEQ ID n ° 1593 Corresponding to the former SEQ ID n ° 1604 Corresponding to the foπner SEQ ID n ° 1979

SEQ ID N ° 3839 Listeria monocytogenes 4b Contig968 Corresponding to the former SEQ ID n ° 1863 Conesponding to the former SEQ ID n ° 1969 SEQ ID N ° 3840 Listeria monocytogenes 4b Contig969 Corresponding to the former SEQ ID n ° 1339 Conesponding to the former SEQ ID No. 1608 Corresponding to the former SEQ ID No. 1942

SEQ ID N ⁰ 3841 Listeria monocytogenes 4b Contig970 Corresponding to the former SEQ ID n ° 2038 SEQ ID N ° 3842 Listeria monocytogenes 4b Contig971 m 00 00 00 00 00 00 00 00 00 00 00 00 00 en en en en en en en en en en en

OOO | _ _^ OOOO 0 OO 0 0 O 0

| __ | | _ _^ | _ _^

O σ σ σ 0 σ σ σ σ σ σ σ σ σ z o z z z Z z z z z z z z z z z

OJ OJ OJ OJ OJ OJ OJ OJ OJ J OJ OJ OJ OJ

00 00 00 00 00 00 00 00 00 00 00 00 CX ⁾ 00

01 On n on on on 42. 42. 42- 42. 42. 4 ^

ON On 42. OJ ro O NO 00 ^ 1 on 42. OJ

oooorororooo oooroororooorooroooo ooooroo ooooe-00

ON Ό 42. OJ OJ ON 00 J on 00 -0 ON ^ 1 NO -t ^ L on -P- -P- 00 NO 0 ON -P- -O ro on OJ on ro 0 00 0 OJ ON O ro 42 NO NO NO 42. 00 OJ on 00 NO ON ro _' > NO ro> - • OO -! on -J 4 ^ ro 42. ON

Corresponding to the former SEQ ID No. 1682 Corresponding to the former SEQ ID No. 2031

SEQ ID No. 3857 Listeria monocytogenes 4b Contig986 Corresponding to the former SEQ ID No. 1468 Corresponding to the former SEQ ID No. 1606 Corresponding to the former SEQ ID No. 1930

SEQ ID N ° 3858 Listeria monocytogenes 4b Contig987 Corresponding to the former SEQ ID n ° 1740 Corresponding to the fonner SEQ ID n ° 2034

SEQ ID N ° 3859 Listeria monocytogenes 4b Contig988 Corresponding to the former SEQ ID n ° 1 156 Corresponding to the foπner SEQ ID n ° 1241 Corresponding to the former SEQ ID n ° 1715 Corresponding to the former SEQ ID n ° 1958

SEQ ID N ° 3860 Listeria monocytogenes 4b Contig989 Corresponding to the former SEQ ID n ° 1 158 Corresponding to the foπner SEQ ID n ° 1719 Corresponding to the former SEQ ID n ° 2023

SEQ ID N ⁰ 3861 Listeria monocytogenes 4b Contig990 Corresponding to the former SEQ ID n ° 1 1 10 Corresponding to the former SEQ ID n ° 191 1 Corresponding to the former SEQ ID n ° 2022

SEQ ID N ° 3862 Listeria monocytogenes 4b Contig991 Corresponding to the former SEQ ID n ° 1 190 Corresponding to the former SEQ ID n ° 1735 Corresponding to the foπner SEQ ID n ° 1823 Corresponding to the former SEQ ID n ° 1824

SEQ ID N ° 3863 Listeria monocytogenes 4b Contig992 Corresponding to the foπner SEQ ID n ° 1257 Corresponding to the former SEQ ID n ° 1907 Corresponding to the former SEQ ID n ° 1989

SEQ ID N ° 3864 Listeria monocytogenes 4b Contig993 Corresponding to the former SEQ ID n ° 1802 Corresponding to the former SEQ ID n ° 1803 Corresponding to the fonner SEQ ID n ° 1833 Coπ-esponding to the former SEQ ID n ° 2016

SEQ ID N ⁰ 3865 Listeria monocytogenes 4b Contig994 Corresponding to the former SEQ ID n ° 1879 Corresponding to the former SEQ ID n ° 1924 Corresponding to the former SEQ ID n ° 1977 Corresponding to the former SEQ ID n ° 1993

SEQ ID N ° 3866 Listeria monocytogenes 4b Contig995 Corresponding to the former SEQ ID n ° 1390 Corresponding to the former SEQ ID n ° 1834 Corresponding to the former SEQ ID n ° 1876

SEQ ID N ° 3867 Listeria monocytogenes 4b Contig996 Corresponding to the foπner SEQ ID n ° 1 192 Coπ-esponding to the former SEQ ID n ° 1591 Corresponding to the former SEQ ID n ° 1712

SEQ ID N ° 3868 Listeria monocytogenes 4b Contig997 Corresponding to the former SEQ ID No. 1964 Corresponding to the former SEQ ID No. 2032

SEQ ID N ° 3869 Listeria monocytogenes 4b Contig998 Conesponding to the former SEQ ID n ° 1287 Corresponding to the former SEQ ID n ° 1430 Conesponding to the former SEQ ID n ° 1678 Corresponding to the former SEQ ID n ° 1902 Corresponding to the foπner SEQ ID No. 1940

SEQ ID N ° 3870 Listeria monocytogenes 4b Contig999 Corresponding to the former SEQ ID n ° 1357 Corresponding to the former SEQ ID n ° 1646 Conesponding to the former SEQ ID n ° 1887 Corresponding to the former SEQ ID n ° 1995

SEQ ID N ⁰ 3871 Listeria monocytogenes 4b Contig 1000 Corresponding to the former SEQ ID n ° 1202 Corresponding to the former SEQ ID n ° 1724 Corresponding to the former SEQ ID n ° 2036

SEQ ID N ⁰ 3872 Listeria monocytogenes 4b Contig 1001 Corresponding to the former SEQ ID n 2039 SEQ ID N ° 3873 Listeria monocytogenes 4b Contigl 002 Corresponding to the former SEQ ID n ° 113 Corresponding to the former SEQ ID n ° 429 Corresponding to the former SEQ ID No. 600 Corresponding to the former SEQ ID No. 956

SEQ ID N ° 3874 Listeria monocytogenes 4b Contig 1003 Corresponding to the former SEQ ID n ° 214 Corresponding to the former SEQ ID n ° 817

SEQ ID N ° 3875 Listeria monocytogenes 4b Contigl 004 Corresponding to the foπner SEQ ÏD n ° 249 Corresponding to the former SEQ ID n ° 378 Corresponding to the former SEQ ID n ° 463 Corresponding to the former SEQ ID n ° 708 Corresponding to the former SEQ ID # 749

SEQ ID N ° 3876 Listeria monocytogenes 4b Contigl 005 Corresponding to the foπner SEQ ID n ° 544 Corresponding to the former SEQ ID n ° 723 Corresponding to the former SEQ ID n ° 738 Corresponding to the fonner SEQ ID n ° 2015

SEQ ID N ° 3877 Listeria monocytogenes 4b Contigl 006 Corresponding to the former SEQ ID n ° 516 Conesponding to the former SEQ ID n ° 746 Corresponding to the former SEQ ID n ° 830

SEQ ID N ° 3878 Listeria monocytogenes 4b Contig 1007 Corresponding to the former SEQ ID n ° 467 Corresponding to the former SEQ ID n ° 806 Conesponding to the foπner SEQ ID n ° 811 Corresponding to the former SEQ ID n ° 837

SEQ ID No. 3879 Listeria monocytogenes 4b Contig 1008 Corresponding to the foπner SEQ ID No. 644 Conesponding to the former SEQ ID No. 702 Corresponding to the former SEQ ID No 1990 Corresponding to the foπner SEQ ID No 1996

SEQ ID N ⁰ 3880 Listeria monocytogenes 4b Contigl 009 Corresponding to the former SEQ ID n ° 1565 Corresponding to the former SEQ ID n ° 1595 Corresponding to the former SEQ ID n ° 1918

SEQ ID N ⁰ 3881 Listeria monocytogenes 4b Contigl 010 Corresponding to the former SEQ ID n ° 1361 Corresponding to the foπner SEQ ID n ° 1478 Corresponding to the former SEQ ID n ° 1510 Corresponding to the former SEQ ID n ° 2006

SEQ ID N ° 3882 Listeria monocytogenes 4b Contigl 01 1 Corresponding to the former SEQ ID n ° 1083 Corresponding to the former SEQ ID n ° 2037

SEQ ID N ⁰ 3883 Listeria monocytogenes 4b Contigl012 Corresponding to the former SEQ ID n ° 1769 Corresponding to the former SEQ ID n ° 1835 Coπ-esponding to the former SEQ ID n ° 1850 Corresponding to the former SEQ ID n ° 1867

SEQ ID N ° 3884 Listeria monocytogenes 4b Contigl 013 Corresponding to the foπner SEQ ID n ° 1982 Corresponding to the former SEQ ID n ° 2035

SEQ ID N ° 3885 Listeria monocytogenes 4b Contigl 014 Corresponding to the former SEQ ID n ° 1529 Corresponding to the former SEQ ID n ° 1581 Corresponding to the former SEQ ÏD n ° 1739 Corresponding to the former SEQ ID n ° 1865 Corresponding to the former SEQ ID No. 1970

SEQ ID N ⁰ 3886 Listeria monocytogenes 4b Contig] 015 Conesponding to the former SEQ ID n ° 1 170 Corresponding to the former SEQ ID n ° 1 180 Corresponding to the former SEQ ID n ° 1265 Corresponding to the former SEQ ID n ° 1434 Corresponding to the former SEQ ID No. 1536 Corresponding to the former SEQ ID No. 1548 Corresponding to the former SEQ ID No. 1877

SEQ ID N ° 3887 Listeria monocytogenes 4b Contigl 016 Corresponding to the former SEQ ID n ° 1219 Conesponding to the former SEQ ID n ° 1917 Corresponding to the former SEQ ID n ° 2040

SEQ ID N ⁰ 3888 Listeria monocytogenes 4b Contig 1017 Corresponding to the former SEQ ID n ° 1245 Corresponding to the former SEQ ID n ° 1821 Conesponding to the former SEQ ID n ° 1841 Corresponding to the fonner SEQ ID n ° 2004 Corresponding to the former SEQ ID No.2025

SEQ ID N ⁰ 3889 Listeria monocytogenes 4b Contigl 018 Corresponding to the foπner SEQ ID n ° 1317 Corresponding to the former SEQ ID n ° 1813 Corresponding to the former SEQ ÏD n ° 1991 Corresponding to the former SEQ ID No. 2001 Corresponding to the former SEQ ID No. 2029

SEQ ID N ° 3890 Listeria monocytogenes 4b Contigl019 Corresponding to the foπner SEQ ID n ° 1926 Corresponding to the former SEQ ID n ° 2041

SEQ ID N ⁰ 3891 Listeria monocytogenes 4b Contig 1020 Corresponding to the foπner SEQ ID n ° 1873

TABLE IX: Legends

SEQ ID Nos. 3892-4025: sequences of 134 Contigs from the assembly of 13919 sequences of Listeria monocytogenes 4b after subtraction of the sequences of L.monocytogenes EGDe and L. innocua Clipl 1262.

TABLE IX

SEQ ID N ° 3892 Listeria monocytogenes 4b specific Contigό

SEQ ID N ° 3893 Listeria monocytogenes 4b specific Contig7

SEQ ID N ° 3894 Listeria monocytogenes 4b specific Contig8

SEQ ID N ° 3895 Listeria monocytogenes 4b specific Contig9

SEQ ID N ⁰ 3896 Listeria monocytogenes 4b specific Contig 10

SEQ ID N ° 3897 Listeria monocytogenes 4b specific Contigl 1

SEQ ID N ⁰ 3898 Listeria monocytogenes 4b specific Contig 12

SEQ ID N ° 3899 Listeria monocytogenes 4b specific Contig 13

SEQ ID N ° 3900 Listeria monocytogenes 4b specific Contig 14

SEQ ID N ⁰ 3901 Listeria monocytogenes 4b specific Contigl 5

SEQ ID N ° 3902 Listeria monocytogenes 4b specific Contig 16

SEQ ID N ° 3903 Listeria monocytogenes 4b specific Contigl 7

SEQ ID N ° 3904 Listeria monocytogenes 4b specific Contigl 8

SEQ ID N ° 3905 Listeria monocytogenes 4b specific Contig 19

SEQ ID N ° 3906 Listeria monocytogenes 4b specific Contig20

SEQ ID N ° 3907 Listeria monocytogenes 4b specific Contig21

SEQ ID N ° 3908 Listeria monocytogenes 4b specific Contig22

SEQ ID N ° 3909 Listeria monocytogenes 4b specific Contig23

SEQ ID N ⁰ 3910 Listeria monocytogenes 4b specific Contig24

SEQ ID N ⁰ 391 1 Listeria monocytogenes 4b specific Contig25

SEQ ID N ⁰ 3912 Listeria monocytogenes 4b specific Contig26

SEQ ID N ⁰ 3913 Listeria monocytogenes 4b specific Contig27

SEQ ID N ⁰ 3914 Listeria monocytogenes 4b specific Contig28

SEQ ID N ⁰ 3915 Listeria monocytogenes 4b specific Contig29

SEQ ID N ⁰ 3916 Listeria monocytogenes 4b specific Contig30

SEQ ID N ⁰ 3917 Listeria monocytogenes 4b specific Contig31

SEQ ID N ⁰ 3918 Listeria monocytogenes 4b specific Contig32

SEQ ID N ⁰ 3919 Listeria monocytogenes 4b specific Contig33

SEQ ID N ° 3920 Listeria monocytogenes 4b specific Contig34

SEQ ID N ⁰ 3921 Listeria monocytogenes 4b specific Contig35 mmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmmm en enenen en en en en en en eπ en rt eπ en en erterteπ erterten en erten en en en en fnen ertfnw ooooooooooooooooooooooooooozzoozzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzzz

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SEQ ID N ° 4022 Listeria monocytogenes 4b-specific Contig 136

SEQ ID N ° 4023 Listeria monocytogenes 4b-specific Contig 137

SEQ ID N ° 4024 Listeria monocytogenes 4b-specific Contigl 38

SEQ ID N ° 4025 Listeria monocytogenes 4b-specific Contig 139

Claims

1. Method for identifying nucleotide sequences specific for the genome of a strain of bacteria of the genus Listeria, in particular specific for a strain of L. innocua or L. monocytogenes, such as the strain L. monocytogenes EGDe or L. monocytogenes 4b .

2. Method for identifying nucleotide sequences according to claim 1, characterized in that the specific sequences of:

- L. monocytogenes EGDe compared to L. innocua and / or L. monocytogenes 4b; or - L. monocytogenes 4b compared to L. innocua and / or L. monocytogenes EGDe.

3. Method for identifying nucleotide sequences according to claim 1 or 2, characterized in that it comprises at least the following steps: a) alignment of the nucleotide sequences of L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b, and those of L. innocua according to claims 5 to 8, 10 to 17 and 21; and b) processing the data obtained by this alignment to isolate said specific sequences.

4. Method for identifying nucleotide sequences according to one of claims 1 to 3, characterized in that the nucleotide sequences specific for L. inocua or L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b , hybridize under high stringency conditions respectively with the nucleotide sequences, or their complementary sequence, of L. inocua or L. monocytogenes, in particular those of L. monocytogenes EGDe and / or L. monocytogenes 4b.

5. Nucleotide sequence derived from Listeria innocua characterized in that it corresponds to a sequence chosen from SEQ ID No. 1 to SEQ ID No. 1 1, SEQ

ID No. 2057 and SEQ ID No. 2058.

6. Nucleotide sequence derived from Listeria innocua, characterized in that it is chosen from: a) a nucleotide sequence comprising at least 75% identity with a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058; b) a nucleotide sequence hybridizing under high stringency conditions with a sequence chosen from SEQ ID No. 1 to SEQ ID No. 1 1, SEQ ID

No. 2057 and SEQ ID No. 2058; c) a nucleotide sequence complementary to a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058 or complementary to a nucleotide sequence as defined in a) or b), or a nucleotide sequence of the RNA corresponding to one of the sequences a) or b); d) a nucleotide sequence of a fragment representative of a sequence chosen from SEQ ID No. 1 to SEQ ID No. 11, SEQ ID No. 2057 and SEQ ID No. 2058, or of a fragment representative of a nucleotide sequence as defined in a), b) or c); e) a nucleotide sequence comprising a sequence as defined in a), b), c) or d); and f) a nucleotide sequence as defined in a), b), c), d) or e) modified.

7. Nucleotide sequence according to claim 6, characterized in that it is a sequence resulting from a sequence chosen from SEQ ID No. 1 to SEQ ID No.

1 1, SEQ ID No. 2057 and SEQ ID No. 2058, and in that it codes for a polypeptide, said nucleotide sequence preferably being chosen from sequences SEQ ID

No. 12 to SEQ ID No. 689, SEQ ID No. 2053 to SEQ ID No. 2056 and SEQ ID No. 2059 to

SEQ ID No. 2601.

8. Nucleotide sequence characterized in that it comprises a nucleotide sequence chosen paπni: a) a nucleotide sequence according to claim 7; b) a nucleotide sequence comprising at least 75% identity with a nucleotide sequence according to claim 7; c) a nucleotide sequence hybridizing under conditions of high stringency with a nucleotide sequence according to claim 7; d) a complementary nucleotide or RNA sequence corresponding to a sequence as defined in a), b) or c); e) a nucleotide sequence of a fragment representative of a sequence as defined in a), b), c) or d); and f) a sequence as defined in a), b), c), d) or e) modified.

9. Polypeptide encoded by a nucleotide sequence according to one of claims 6 to 8.

10. Polypeptide according to claim 9, characterized in that it is chosen from polypeptides coded by a sequence chosen from SEQ ID No. 12 to SEQ ID No. 689, SEQ ID No. 2053 to SEQ ID No. 2056 and SEQ ID No. 2059 to SEQ ID No. 2601.

1 1. A polypeptide characterized in that it comprises a polypeptide chosen from: a) a polypeptide according to one of claims 9 and 10; b) a polypeptide having at least 80% identity with a polypeptide according to one of claims 9 and 10; c) a fragment of at least 5 amino acids of a polypeptide according to one of claims 9 and 10, or as defined in b); d) a biologically active fragment of a polypeptide according to one of claims 9 and 10, or as defined in b) or c); and e) a polypeptide according to one of claims 9 and 10, or as defined in b), c) or d) modified.

12. Nucleotide sequence coding for a polypeptide according to claim

1 1.

13. Nucleotide sequence coding for a polypeptide specific for L. innocua, characterized in that it is chosen paπni SEQ ID No. 12 to SEQ ID No. 689 and SEQ ID No. 2059 to SEQ ID No. 2601.

14. Nucleotide sequence derived from Listeria monocytogenes serotype 4b characterized in that it corresponds to a chosen sequence paπni SEQ ID No. 1068 to

SEQ ID No. 2041 and SEQ ID No. 2872 to SEQ ID No. 3891.

15. Nucleotide sequence derived from Listeria monocytogenes serotype 4b, characterized in that it is chosen paπni: a) a nucleotide sequence comprising at least 75% identity with a sequence chosen from SEQ ID No. 1068 to SEQ ID No. 2041 and SEQ ID No. 2872 to

SEQ ID No. 3891; b) a nucleotide sequence hybridizing under conditions of high stringency with a chosen sequence of SEQ ID No. 1068 to SEQ ID No. 2041 and SEQ ID No. 2872 to SEQ ID No. 3891; c) a nucleotide sequence complementary to a sequence chosen from SEQ ID No. 1068 to SEQ ID No. 2041 and SEQ ID No. 2872 to SEQ ID No. 3891 or complementary to a nucleotide sequence as defined in a), or b), or a nucleotide sequence of the RNA corresponding to one of the sequences a) or b); d) a nucleotide sequence of a fragment representative of a sequence chosen from SEQ ID No. 1068 to SEQ ID No. 2041 and SEQ ID No. 2872 to SEQ ID No. 3891, or of a fragment representative of a sequence nucleotide as defined in a), b) or c); e) a nucleotide sequence comprising a sequence as defined in a), b), c) or d); and f) a nucleotide sequence as defined in a), b), c), d) or e) modified.

16. Nucleotide sequence according to claim 15, characterized in that it is a sequence resulting from a sequence chosen paπni SEQ ID No. 1068 to SEQ ID No. 2041 and SEQ ID No. 2872 to SEQ ID No 3891, and in that it codes for a polypeptide, said nucleotide sequence being preferably chosen from the sequences SEQ ID No. 690 to SEQ ID No. 1067 and SEQ ID No. 2602 to SEQ ID No. 2871 and SEQ ID No. 2049 to SEQ ID No. 2052.

17. Nucleotide sequence, characterized in that it comprises a nucleotide sequence chosen from: a) a nucleotide sequence according to claim 16; b) a nucleotide sequence comprising at least 75% identity with a nucleotide sequence according to claim 16; c) a nucleotide sequence hybridizing under conditions of high stringency with a nucleotide sequence according to claim 16; d) a complementary nucleotide or RNA sequence corresponding to a sequence as defined in a), b) or c); e) a nucleotide sequence of a fragment representative of a sequence as defined in a), b), c) or d); and f) a sequence as defined in a), b), c), d) or e) modified.

18. Polypeptide encoded by a nucleotide sequence according to one of claims 15 to 17.

19. Polypeptide according to claim 18, characterized in that it is chosen from polypeptides coded by a sequence chosen from SEQ ID No. 690 to SEQ ID No. 1067, SEQ ID No. 2049 to SEQ ID No. 2052 and SEQ ID No. 2602 to SEQ ID No. 2871.

20. Polypeptide characterized in that it comprises a polypeptide chosen from: a) a polypeptide according to one of claims 18 and 19; b) a polypeptide having at least 80% identity with a polypeptide according to one of claims 18 and 19; c) a fragment of at least 5 amino acids of a polypeptide according to one of claims 18 and 19, or as defined in b); d) a biologically active fragment of a polypeptide according to one of claims 18 and 19, or as defined in b) or c); and e) a polypeptide according to one of claims 18 and 19 or as defined in b), c) or d) modified.

21. Nucleotide sequence coding for a polypeptide according to claim 20.

22. Nucleotide sequence coding for a polypeptide specific for L. monocytogenes, characterized in that it is chosen from SEQ ID No. 690 to SEQ ID

No. 1067, SEQ ID No. 2602 to SEQ ID No. 2871 and SEQ ID No. 3892 to SEQ ID No. 4025.

23. Nucleotide sequence coding for a polypeptide having at least 87% identity between L. innocua and L. monocytogenes, characterized in that it is chosen from SEQ ID No. 2049 to SEQ ID No. 2056.

24. Nucleotide sequence according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 23, characterized in that it codes for a polypeptide of L. innocua or L. monocytogenes or one of its fragments:

- involved in the biosynthesis of amino acids in the biosynthesis of cofactors, prosthetic groups and transporters;

- cell envelope or located on the surface of L. innocua or L. monocytogenes;

- involved in cellular machinery;

- involved in central intermediate metabolism;

- involved in energy metabolism; - involved in the metabolism of fatty acids and phospholipids;

- involved in the metabolism of nucleotides, purines, pyrimidines or nucleosides;

- involved in regulatory functions; - involved in the replication process;

- involved in the transcription process;

- involved in the translation process;

- involved in the protein transport and binding process;

- involved in adapting to atypical conditions; - involved in sensitivity to drugs and the like; or

- involved in the functions relating to transposons.

25. Polypeptide according to one of claims 9 to 1 1, and 17 to 20, characterized in that it is a polypeptide deE innocua or L. monocytogenes:

- cell envelope or located on the surface of L. innocua or L. monocytogenes;

- involved in cellular machinery;

- involved in central intermediate metabolism;

- involved in regulatory functions;

- involved in the replication process; - involved in the transcription process;

- involved in the translation process;

- involved in the protein transport and binding process;

- involved in adapting to atypical conditions;

- involved in sensitivity to drugs and the like; or - involved in the functions relating to transposons.

26. Nucleotide sequence usable as a primer or as a probe, characterized in that said sequence is chosen from the nucleotide sequences according to one of claims 6 to 8, 12 to 17 and 21 to 23.

27. Nucleotide sequence according to claim 26, characterized in that it is marked by a radioactive compound or by a non-radioactive compound.

28. Nucleotide sequence according to one of claims 26 and 27, characterized in that it is immobilized on a support, covalently or non-covalently.

29. Nucleotide sequence according to claim 28, characterized in that it is immobilized on a support such as a high density filter or a DNA chip.

30. Nucleotide sequence according to one of claims 27 to 29 for the detection and / or amplification of nucleic sequences.

31. DNA chip or filter, characterized in that it contains at least one nucleotide sequence according to claim 29.

32. DNA chip or filter according to claim 31, characterized in that it also contains at least one nucleotide sequence of a microorganism other than L. innocua or L. monocytogenes, immobilized on the support of said chip.

33. DNA chip or filter according to claim 32, characterized in that the other microorganism is chosen from a microorganism associated with L. innocua or L. monocytogenes, a bacterium of the genus Listeria, and a variant of L. innocua or L. monocytogenes.

34. Kit or kit for the detection and / or identification of bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism, characterized in that it comprises a DNA chip or a filter according to claim 31.

35. Kit or kit for the detection and / or identification of a microorganism, characterized in that it comprises a DNA chip or a filter according to one of claims 32 and 33.

36. Kit or kit for the detection and / or quantification of the expression of at least one gene of L. innocua or L. monocytogenes, characterized in that it comprises a DNA chip or a filter according to one from claims 32 to 33.

37. Cloning and / or expression vector, characterized in that it contains a nucleotide sequence according to one of claims 5 to 8, 12,13, 15 to 17 and 21 to 23.

38. Host cell, characterized in that it is transformed by a vector according to claim 37.

39. Host cell according to claim 38, characterized in that it is a bacterium belonging to the genus Listeria.

40. Host cell according to claim 39, characterized in that it is a bacterium belonging to the species L. innocua or L. monocytogenes.

41. Plant or animal, except Man, comprising a transformed cell according to one of claims 38 to 40.

42. A method of preparing a polypeptide, characterized in that a cell transformed with a vector according to claim 37 is cultured under conditions allowing the expression of said polypeptide and that said recombinant polypeptide is recovered.

43. Recombinant polypeptide obtainable by a method according to claim 42.

44. Process for preparing a synthetic polypeptide according to one of claims 9 to 11, 18 to 20 and 25, characterized in that a chemical synthesis of said polypeptide is carried out.

45. Hybrid polypeptide, characterized in that it comprises at least the sequence of a polypeptide according to one of claims 9 to 11, 18 to 20, 25 and 43, and a sequence of a polypeptide capable of inducing an immune response in humans or animals.

46. Nucleotide sequence coding for a hybrid polypeptide according to claim 45.

47. Vector characterized in that it contains a nucleotide sequence according to claim 46.

48. Monoclonal or polyclonal antibody, its fragments, or chimeric antibody, characterized in that it is capable of specifically recognizing a polypeptide according to one of claims 9 to 11, 18 to 20, 25, 43 and 45.

49. Antibody according to claim 48, characterized in that it is a labeled antibody.

50. Method for the detection and / or identification of bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism in a biological sample, characterized in that it comprises the following steps: a ) bringing the biological sample into contact with an antibody according to one of claims 48 and 49; b) highlighting of the antigen-antibody complex possibly formed.

51. Method for detecting the expression of a gene for L. innocua or L. monocytogenes, characterized in that a strain of L. innocua or L. monocytogenes is brought into contact with an antibody according to claim 74 or 75 and that the antigen / antibody complex possibly formed is detected.

52. Kit or kit for the implementation of a method according to claim 50 or 51, characterized in that it comprises the following elements: a) an antibody according to one of claims 48 and 49; b) optionally, the reagents for constituting the medium suitable for the immunological reaction; c) optionally, the reagents allowing the detection of the antigen-antibody complexes produced by the immunological reaction.

53. Polypeptide according to one of claims 9 to 11, 18 to 20, 25, 43 and 45, or antibody according to one of claims 48 and 49, characterized in that it is immobilized on a support, in particular a protein chip.

54. Protein chip, characterized in that it contains at least one polypeptide according to one of claims 9 to 1 1, 18 to 20, 25, 43 and 45, or at least one antibody according to one of claims 48 and 49, immobilized on the support of said chip.

55. Protein chip according to claim 54, characterized in that it additionally contains at least one polypeptide of a microorganism other than L. innocua or

L. monocytogenes or at least one antibody directed against a microorganism compound other than L. innocua or L. monocytogenes, immobilized on the support of said chip.

56. Kit or kit for the detection and / or identification of bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism, characterized in that it comprises a protein chip according to the one of claims 54 and 55.

57. Kit or kit for the detection and / or identification of a microorganism, characterized in that it comprises a protein chip according to claim 56.

58. Method for detecting and / or identifying bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism in a biological sample, characterized in that it implements a nucleotide sequence according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24, 26 to 30 and 46.

59. Method according to claim 58, characterized in that it comprises the following steps: a) optionally, isolation of the DNA from the biological sample to be analyzed, or obtaining a cDNA from the RNA biological sample; b) specific amplification of the DNA of bacteria belonging to the species L. innocua or L. monocytogenes or to a microorganism associated with the aid of at least one primer according to one of claims 26 to 30; c) highlighting of the amplification products.

60. Method according to claim 58, characterized in that it comprises the following steps: a) bringing a nucleotide probe according to one of claims 26 to 30 into contact with a biological sample, the nucleic acid contained in the biological sample having, where appropriate, previously made accessible to hybridization, under conditions permitting hybridization of the probe to the nucleic acid of a bacterium belonging to the species L. innocua or L. monocytogenes or to an associated microorganism; b) highlighting of the hybrid possibly formed between the nucleotide probe and the nucleic acid of the biological sample.

61. Method according to claim 58, characterized in that it comprises the following steps: a) bringing a nucleotide probe immobilized on a support according to claim 28 into contact with a biological sample, the nucleic acid of the sample having, where appropriate, previously been made accessible for hybridization, under conditions allowing hybridization of the probe to the nucleic acid of a bacterium belonging to the species L. innocua or L. monocytogenes or to a microorganism partner; b) bringing the hybrid formed into contact between the nucleotide probe immobilized on a support and the nucleic acid contained in the biological sample, if appropriate after elimination of the nucleic acid from the non-hybridized biological sample with the probe, with a labeled nucleotide probe according to claim 27; c) highlighting of the new hybrid formed in step b).

62. Method according to claim 61, characterized in that, before step a), the DNA of the biological sample or the cDNA optionally obtained by reverse transcription of the sample RNA, is amplified using at least one primer according to one of claims 26 to 30.

63. Kit or kit for the detection and / or identification of bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism, characterized in that it comprises the following elements: a) a nucleotide probe according to one of claims 26 to 30; b) optionally, the reagents necessary for carrying out a hybridization reaction; c) optionally, at least one primer according to one of claims 26 to 30 as well as the reagents necessary for a DNA amplification reaction.

64. Kit or kit for the detection and / or identification of bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism, characterized in that it comprises the following elements: a) a nucleotide probe, said capture probe, according to claim 28; b) an oligonucleotide probe, said revelation probe, according to claim 27; c) optionally, at least one primer according to one of claims 26 to 30 as well as the reagents necessary for a DNA amplification reaction.

65. Kit or kit for the detection and / or identification of bacteria belonging to the species L. innocua or L. monocytogenes or to an associated microorganism, characterized in that it comprises the following elements: a) at least one primer according to one of claims 26 to 30; b) optionally, the reagents necessary to carry out a DNA amplification reaction; c) optionally, a component making it possible to verify the sequence of the amplified fragment, more particularly an oligonucleotide probe according to one of claims 26 to 30.

66. Method according to claims 50, 51 and 58 to 62 or kit or necessary according to claims 52, 56, 57 and 63 to 65 for the detection and / or identification of bacteria belonging to the species L. innocua or L monocytogenes, characterized in that said primer and / or said probe are chosen from the nucleotide sequences according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24, 26 to 30 and 46 specific for l species L. innocua or L. monocytogenes, in that said polypeptides are chosen from the polypeptides according to one of claims 9 to 11, 18 to 20, 25, 43 and 45 specific for the species L. innocua or L. monocytogenes and in that the said antibodies are chosen by the antibodies according to one of claims 48 and 49 directed against the polypeptides chosen from the polypeptides according to one of the claims 9 to 1 1 , 18 to 20, 25, 43 and 45 specific to the species L. innocua or L. monocytogenes.

67. Strain of L. innocua or L. monocytogenes, characterized in that it contains at least one mutation in at least one nucleotide sequence according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24 .

68. A strain of L. innocua or L. monocytogenes according to claim 67, characterized in that the mutation leads to an inactivation of the gene.

69. A strain of L. innocua or L. monocytogenes according to claim 67, characterized in that the mutation leads to an overexpression of the gene.

70. Use of a nucleotide sequence according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24, of a polypeptide according to one of claims 9 to 1 1, 18 to 20, 25 , 43 and 45, an antibody according to one of claims 48 and 49, a cell according to one of claims 38 to 40, and / or an animal transformed according to claim 41 for the selection of compound organic or inorganic capable of modulating, regulating, inducing or inhibiting gene expression, and / or modifying cellular replication of eukaryotic or prokaryotic cells or capable of inducing, inhibiting or aggravating in an animal or human organism pathologies linked to an infection by E. monocytogenes or by an associated microorganism.

71. Method for selecting a compound capable of binding to a polypeptide according to one of claims 9 to 11, 18 to 20, 25, 43 and 45, capable of binding to a nucleotide sequence according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24, or capable of recognizing an antibody according to one of claims 48 and 49, and / or capable of modulating, regulating, inducing or inhibiting l expression of genes, and / or modifying cellular replication of eukaryotic or prokaryotic cells, or capable of inducing, inhibiting or aggravating in an animal or human organism the pathologies linked to an infection with L. monocytogenes, characterized in that it comprises the following stages: a) bringing said compound into contact with said polypeptide, said nucleotide sequence, with a transformed cell according to one of claims 38 to 40, and / or administration of said compound to an animal transfected according to claim 41; b) determining the capacity of said compound to bind with said polypeptide or said nucleotide sequence, or to modulate, regulate, induce or inhibit the expression of genes, or to modulate cell growth or replication, or to induce, inhibit or aggravate in said animal or human organism the pathologies linked to an infection by L. monocytogenes or by a microorganism associated.

72. Pharmaceutical composition comprising a compound chosen from the following compounds: a) a nucleotide sequence according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24; b) a polypeptide according to one of claims 9 to 11, 18 to 20, 25, 43 and 45; c) a vector according to claim 37 or 47; d) an antibody according to claim 48 or 49.

73. Composition according to claim 72, optionally in combination with a pharmaceutically acceptable vehicle.

74. Pharmaceutical composition according to one of claims 72 and 73 for the prevention or treatment of an infection by a bacterium belonging to the species L. monocytogenes or by an associated microorganism.

75. Immunogenic composition, characterized in that it comprises one or more polypeptides according to one of claims 9 to 11, 18 to 20, 25, 43, and / or one or more hybrid polypeptides according to claim 45.

76. Use of a cell according to one of claims 38 to 40, or of a vector according to one of claims 37 or 47 for the preparation of a vaccine composition.

77. Vaccine composition, characterized in that it contains a polynucleotide according to one of claims 6 to 8, 12, 13, 15 to 17, 21 to 24, a vector according to one of claims 37 or 47, and / or a cell according to one of claims 38 to 40.

78. Immunogenic composition capable of inducing a cellular or humoral immune response for the prevention or treatment of an infection by bacteria belonging to the species L. monocytogenes or by an associated microorganism, characterized in that it comprises a immunogenic composition according to either of Claims 75 and 77, in combination with a pharmaceutically acceptable vehicle and, optionally, one or more appropriate immunity adjuvants.

79. Genomic bank of a bacterium of the genus Listeria.

80 Genomic DNA library of a bacterium of the genus Listeria according to claim 79, characterized in that said DNA library is cloned in a plasmid.

81. Genomic DNA bank according to claim 79 or 80, characterized in that said bacterium is L. innocua or L. monocytogenes serotype 4b.

82. Bank according to claim 79 or 80, characterized in that it is the Li-shotgun bank deposited at the CNCM on October 2, 2000 under the number 1-2565.

83. Bank according to claim 79 or 80, characterized in that it is the bank Lm4b-shotgun deposited at the CNCM on October 2, 2000 under the number 1-2566.

84. Genomic library according to claim 79, characterized in that the bacterium is L. innocua or L. monocytogenes.

85. Use of the genomic libraries according to one of claims 79 to 84 for isolating nucleotide sequences specific for L. innocua and L. monocytogenes, characterized in that the nucleotide sequences of L. innocua and L. monocytogenes are aligned and in that that the data obtained by this alignment are processed to isolate said specific sequences.

86. Pharmaceutical composition according to one of claims 72 to 74, characterized in that it comprises antibodies directed against specific polypeptides of L. innocua or L. monocytogenes.

87. Method for identifying specific sequences of L. innocua or L. monocytogenes, characterized by aligning the nucleotide sequences of L. monocytogenes and those of L. innocua according to claims 5 to 8, 12 to 17 and 21 and processing the data obtained by this alignment to isolate said specific sequences.