EP0985453B1 - Chambre de centrifugation pour un séparateur des cellules - Google Patents

Chambre de centrifugation pour un séparateur des cellules Download PDF

Info

Publication number
EP0985453B1
EP0985453B1 EP99117393A EP99117393A EP0985453B1 EP 0985453 B1 EP0985453 B1 EP 0985453B1 EP 99117393 A EP99117393 A EP 99117393A EP 99117393 A EP99117393 A EP 99117393A EP 0985453 B1 EP0985453 B1 EP 0985453B1
Authority
EP
European Patent Office
Prior art keywords
channel
centrifuge chamber
radially
chamber according
separation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP99117393A
Other languages
German (de)
English (en)
Other versions
EP0985453A1 (fr
Inventor
Hans-Jürgen Dr. Neumann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fresenius SE and Co KGaA
Original Assignee
Fresenius SE and Co KGaA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fresenius SE and Co KGaA filed Critical Fresenius SE and Co KGaA
Publication of EP0985453A1 publication Critical patent/EP0985453A1/fr
Application granted granted Critical
Publication of EP0985453B1 publication Critical patent/EP0985453B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04B—CENTRIFUGES
    • B04B5/00—Other centrifuges
    • B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0442—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation
    • B04B2005/045—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers with means for adding or withdrawing liquid substances during the centrifugation, e.g. continuous centrifugation having annular separation channels

Definitions

  • the invention relates to a centrifuge chamber for a cell separator, in particular for the separation of blood into several fractions.
  • the centrifuge chamber of the known cell separators has a Separation channel, in which the cell suspension to be separated is passed. Under the influence of the centrifugal force takes place in the separation channel a Separation into different fractions, for example platelets (PLT), Erythrocytes (RBC), platelet rich plasma (PRP) and platelet-poor plasma (PPP) withdrawn from the chamber.
  • PLT platelets
  • RBC Erythrocytes
  • PRP platelet rich plasma
  • PPP platelet-poor plasma
  • the centrifuge chamber of known cell separators for separating blood into multiple fractions is generally for single use certainly.
  • the separation channel of the known one or two-part Centrifuge chambers is formed in one or more stages.
  • Centrifuge chambers with multi-stage separation channel have the disadvantage that by turbulence in the transition region between the individual Channel sections already entrained separate cells in another fraction can be. For example, there is a risk that already separate Platelets partially or completely mixed with the plasma or Leukocytes are raised as an impurity.
  • Single-stage separation chambers are characterized by unclean ones or insufficient separation especially of platelets, since here the Platelets are obtained from the so-called buffy coat, the naturally heavily contaminated with leukocytes.
  • DE-A-28 21 055 describes a multi-stage centrifuge chamber for Separating blood into several fractions whose separation channel consists of several circular arc sections with different radii, the clearly separated from each other by transition areas or dams.
  • the Sections of the channel differ significantly in their slope, with the Slope of the channel section at the transition point to the itself subsequent section has a break point.
  • a centrifuge chamber whose separation channel consists of several sections is also known from US-A-4,342,420.
  • Of the Separation channel has an outgoing input area, one on a circular path about the axis of rotation extending central region and an on the rotation axis tapered end.
  • US-A-4 356 958 discloses a single-stage separation chamber having a spiral separation channel.
  • the separation channel is designed such that it does not run to the axis of rotation of the chamber, but in the Edge region of the chamber leaking.
  • US 4,479,790 describes a centrifuge chamber with a Separation channel, of a radially inner and a radially outer lying side wall is limited.
  • the course of the separation channel is from a spiral described from the radially outer end of the channel with increasing slope to the radially inner end of the channel extends.
  • the known Zenirifugenhunt is not one Centrifuge chamber for a cell separator, but a centrifuge chamber for Separation of heavy minerals from less dense materials. Consequently, has the chamber also does not have an inlet for a cell suspension to be separated between the radially inner and the radially outer end of Channel.
  • the invention has for its object to provide a centrifuge chamber for a To create a cell separator in which the separation of the cell suspension is very evenly and without contamination takes place.
  • the separation channel may consist of one or more channel sections be composed, with between the individual channel sections Are areas where liquid supplied to the separation chamber or Liquid is discharged. In these areas, the interior and Outside wall of the separation channel naturally have no steady course.
  • the centrifuge chamber according to the invention finds in particular for separation of whole blood into several fractions, namely erythrocytes and / or Platelets and / or plasma use.
  • the separation channel extends up close to the center of the axis of rotation of the centrifuge chamber.
  • the centrifuge chamber is the Outlet for the erythrocyte fraction at the radially outer end of the Channel, while the outlet for the plasma fraction at the radial located inside the end of the channel.
  • the inlet for the too separating cell suspension is preferably between the outlet for the Erythrocyte fraction and the outlet for the plasma fraction arranged.
  • the outlet for the platelet fraction is preferably between the inlet for blood and the outlet for the plasma fraction.
  • the progressive slope of the canal is achieved that Erythrczyten in the radially outer regions of the Channels are not packed too compactly.
  • the hematocrit value of Erythrocytes can therefore in the radially outer areas a Do not exceed the maximum value of 80 to 90% Hct. This is so far from Advantage, as high hematocrit values in the outer areas of the channel radially inward flow of platelets into the plasma with special needs. In addition, it ensures that plasma unhindered over the Whole length of the channel can flow radially inward to the plasma outlet.
  • platelets may be located further inward due to centrifugal force Back areas of the channel to the platelet outlet.
  • the outlet for the Platelets in a spread over the entire height of the separation channel extending recess on the radially outer side wall of the channel arranged, from which the platelets peel off with great effectiveness to let.
  • both the platelets which through the Plasma flow from the buffy coat layer on the erythrocytes to the Plasma outlet be entrained as well as the platelets by the progressive increase of the channel from radially inner areas fall back and get into the depression.
  • the outlet for the platelets is advantageously located in the lower half of the recess, preferably in the radially outer part of the Deepening.
  • the separation channel with the erythrocyte outlet at the radially outside lying and the plasma outlet at the radially inner end can be When prefilling with solutions or bleeding blood slightly, because the air bubbles under the influence of the centrifugal force to the radially inner end be driven where it is removed without residue by the plasma outlet can be.
  • the cross section of the separation channel is over the entire length preferably constant. But it is also possible to use a separation channel to provide a continuously changing in the longitudinal direction cross-section.
  • the centrifuge chamber may be formed as a one-piece chamber, wherein the centrifuge channel is part of the housing body. It is also possible to form the centrifuge chamber in two parts, wherein the Separation channel as a flexible channel of hose or foil material in the Housing body is used.
  • the centrifuge chamber comprises a circular housing body 1, which in the cell separator is inserted.
  • the cell separator rotates in the cell separator Housing body 1 about a vertical axis of rotation 2.
  • the housing body. 1 carries a separation channel 3, which surrounds the axis of rotation 2 of the Centrifuge chamber extends.
  • the separation channel has at its outer end 4 a first Erythrocyte outlet 5 (RWC) and at its inner end 6 a second outlet 7 for plasma (PLS). Between the erythrocyte outlet 5 and the plasma outlet 7, the separation channel 3 has an inlet 8 for the to be separated whole blood (WB), while between the whole blood inlet 8th and the plasma outlet 7, a third outlet 9 for platelets (PLT) is arranged.
  • the inlet and the outlets are in the length of the channel in the essentially arranged distributed at equal intervals.
  • the separation channel 3 has the same cross section along its length. He is from an inner side wall 10 and an outer side Side wall 11 and a lower wall 12 and an upper wall thirteenth limited (Figure 3).
  • the course of the separation channel 3 is by a midway between the Side walls 10, 11 extending line described in the form of a Spiral S around the axis of rotation 2 of the centrifuge chamber winds and on the Rotation axis tapers.
  • the slope of the spiral S describing the course of the rotation channel takes from the outside channel end 4 to the inside channel end 6 towards steadily, with the slope defined at one point on the spiral as the angle between the tangent of a circle around the rotation axis in this point and the tangent of the spiral in this point.
  • FIG 3 is a point on the course of the separation channel descriptive spiral S denoted by A.
  • the slope at the point A is now defined as the angle ⁇ between the Tangent T1 of the circle K in the point A and the tangent T2 of the spiral S, which describes the course of the channel, in the point A.
  • the course of the channel descriptive spiral S has starting from the outer channel end 4 over substantially the first half of its length a pitch that is less than 5 ° and in the second half greater than 5 ° is.
  • the slope parameter y is less than 1500.
  • the whole blood inlet 8 is preferably located at a location of the canal the slope is less than 1 °, while the platelet outlet 9 preferably located at a location of the channel at which the slope is greater than 5 ° is.
  • FIG. 4 shows a section through the separation channel 3 at the height of the Platelet outlet 9. Here runs the outer side wall 11 below Forming a recess 15 radially outward, then again radially to to run inside. At the bottom of the recess is in the outer Side wall of the thrombocyte outlet 9 is arranged.
  • the recess 15 over the entire channel height is formed such that they the channel cross section strömuntgslite not significantly changed and the Flow laminar flows over the trigger.
  • the outer wall of the outside Section of the separation channel goes into an obliquely outward Wall over which an obliquely inward-running wall connects, which then merges into the radially inner channel section.
  • Of the Discharge port for the platelets is located at the point of Separation channel where the two walls meet.
  • the slope parameter y1 is less than 1500 and the slope parameter y2 is less than 10, where phi1 / phi0 is greater than 0.3.
  • the channel can have an angular circumference of> 360 °.
  • Figure 7 shows the course of the separation channel 3, the 270 ° over a very has low slope, which then progressively grows to 540 °.
  • a Separation chamber with such a channel is suitable, a very to gain platelet-rich plasma. This will be on the radially inside lowest point.

Landscapes

  • External Artificial Organs (AREA)
  • Centrifugal Separators (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (11)

  1. Chambre de centrifugation pour un séparateur de cellules, comprenant un canal de séparation (3) composé d'un moins un segment de canal, qui est délimité par deux parois latérales (10, 11), l'une située radialement à l'intérieur et l'autre radialement à l'extérieur, et présentant une entrée (8) pour la suspension cellulaire à séparer, notamment du sang, et au moins une sortie (5) pour une fraction de la suspension cellulaire, l'évolution du ou de chaque segment de canal étant décrite par une ligne qui s'étend au milieu entre les parois latérales et s'enroule autour de l'axe de rotation (2) de la chambre de centrifugation,
       caractérisée en ce que la ligne décrivant l'évolution du ou de chaque segment de canal du canal de séparation (3) est une spirale (S) qui s'étend selon une pente ascendante de l'extrémité de canal située radialement à l'extérieur vers l'extrémité de canal (6) située radialement à l'intérieur, la pente étant définie au niveau d'un point (A) sur la spirale en tant qu'angle α entre la tangente (T1) d'un cercle (K) autour de l'axe de rotation (2) dans ce point et de la tangente (T2) de la spirale dans ce point.
  2. Chambre de centrifugation selon la revendication 1,
       caractérisée en ce que la ligne (S) décrivant l'évolution d'un segment de canal situé radialement à extérieur d'un canal de séparation (3) composé de plusieurs segments de canal présente une pente au niveau de chaque point de la ligne qui est inférieure à la pente au niveau de chaque point d'un segment de canal situé radialement à l'intérieur adjacent au segment de canal situé radialement à l'extérieur.
  3. Chambre de centrifugation selon la revendication 1 ou 2,
       caractérisée en ce que le canal de séparation s'étend jusqu'à proximité de l'axe de rotation (2) de la chambre de centrifugation.
  4. Chambre de centrifugation selon l'une quelconque des revendications 1 à 3,
       caractérisée en ce que la sortie (5) pour une fraction de la suspension cellulaire, notamment la fraction des érythrocytes, est disposée à l'extrémité (4) du canal (3) située radialement à l'extérieur.
  5. Chambre de centrifugation selon l'une quelconque des revendications 1 à 4,
       caractérisée en ce qu'une deuxième sortie (7) pour une deuxième fraction de la suspension cellulaire, notamment la fraction de plasma, est disposée à l'extrémité (6) du canal (3) située radialement à l'intérieur.
  6. Chambre de centrifugation selon l'une quelconque des revendications 1 à 5,
       caractérisée en ce que l'entrée (8) pour la suspension cellulaire à séparer, notamment du sang, est disposée entre les extrémités (6, 4) du canal (3) situées radialement à l'intérieur et radialement à l'extérieur.
  7. Chambre de centrifugation selon la revendication 6,
       caractérisée en ce qu'une troisième sortie (9) pour une troisième fraction de la suspension cellulaire, notamment la fraction des thrombocytes, est disposée entre l'entrée (8) et l'extrémité (6) du canal (3) située radialement à l'intérieur.
  8. Chambre de centrifugation selon la revendication 7,
       caractérisée en ce que la troisième sortie (9) pour la troisième suspension cellulaire, notamment la fraction des thrombocytes, est disposée dans un creux (15) s'étendant pour l'essentiel sur toute la hauteur du canal sur la paroi latérale (11) du canal de séparation (3) située radialement à l'extérieur.
  9. Chambre de centrifugation selon l'une quelconque des revendications 1 à 8,
       caractérisée en ce que l'entrée (8) et les sorties (5, 7, 9) sont réparties sur la longueur du canal de séparation (3) pour l'essentiel à des distances constantes.
  10. Chambre de centrifugation selon l'une quelconque des revendications 1 à 9,
       caractérisée en ce que la spirale (S) décrivant l'évolution du canal de séparation (3) présente, depuis l'extrémité (4) du canal (3) située à l'extérieur, une pente s'étendant pour l'essentiel sur la première moitié de sa longueur, qui est inférieure à 5° et dans la deuxième moitié supérieure à 5°.
  11. Chambre de centrifugation selon l'une quelconque des revendications 1 à 10,
       caractérisée en ce que la section transversale du canal de séparation (3) est constante sur toute la longueur.
EP99117393A 1998-09-12 1999-09-04 Chambre de centrifugation pour un séparateur des cellules Expired - Lifetime EP0985453B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19841835 1998-09-12
DE19841835A DE19841835C2 (de) 1998-09-12 1998-09-12 Zentrifugenkammer für einen Zellseparator

Publications (2)

Publication Number Publication Date
EP0985453A1 EP0985453A1 (fr) 2000-03-15
EP0985453B1 true EP0985453B1 (fr) 2005-11-23

Family

ID=7880779

Family Applications (1)

Application Number Title Priority Date Filing Date
EP99117393A Expired - Lifetime EP0985453B1 (fr) 1998-09-12 1999-09-04 Chambre de centrifugation pour un séparateur des cellules

Country Status (5)

Country Link
US (1) US6277060B1 (fr)
EP (1) EP0985453B1 (fr)
JP (1) JP4027540B2 (fr)
DE (2) DE19841835C2 (fr)
ES (1) ES2248948T3 (fr)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6979307B2 (en) * 1997-06-24 2005-12-27 Cascade Medical Enterprises Llc Systems and methods for preparing autologous fibrin glue
US7745106B2 (en) * 1997-06-24 2010-06-29 Cascade Medical Enterprises, Llc Methods and devices for separating liquid components
EP1186346A1 (fr) * 2000-09-05 2002-03-13 Jean-Denis Rochat Enceinte rotative pour la séparation de composants du sang ou du plasma
WO2002036266A2 (fr) 2000-11-02 2002-05-10 Gambro, Inc. Dispositif de separation fluidique, systemes et/ou procedes utilisant une pression fluidique commandee et/ou equilibree
US6890291B2 (en) 2001-06-25 2005-05-10 Mission Medical, Inc. Integrated automatic blood collection and processing unit
US7585670B2 (en) 2001-12-07 2009-09-08 Cytori Therapeutics, Inc. Automated methods for isolating and using clinically safe adipose derived regenerative cells
US7771716B2 (en) 2001-12-07 2010-08-10 Cytori Therapeutics, Inc. Methods of using regenerative cells in the treatment of musculoskeletal disorders
US8105580B2 (en) 2001-12-07 2012-01-31 Cytori Therapeutics, Inc. Methods of using adipose derived stem cells to promote wound healing
US7651684B2 (en) 2001-12-07 2010-01-26 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in augmenting autologous fat transfer
US9597395B2 (en) 2001-12-07 2017-03-21 Cytori Therapeutics, Inc. Methods of using adipose tissue-derived cells in the treatment of cardiovascular conditions
US20050095228A1 (en) 2001-12-07 2005-05-05 Fraser John K. Methods of using regenerative cells in the treatment of peripheral vascular disease and related disorders
US20030161816A1 (en) 2001-12-07 2003-08-28 Fraser John K. Systems and methods for treating patients with processed lipoaspirate cells
US6982038B2 (en) * 2002-06-14 2006-01-03 Medtronic, Inc. Centrifuge system utilizing disposable components and automated processing of blood to collect platelet rich plasma
MXPA05009044A (es) 2003-02-20 2006-03-17 Macropore Biosurgery Inc Metodos para utilizar celulas derivadas de tejido adiposo en el tratamiento de condiciones cardiovasculares.
TW200607567A (en) * 2004-08-05 2006-03-01 Filtra Ltd A low shear centrifugal separator
JP4368383B2 (ja) * 2004-10-01 2009-11-18 株式会社アドバンス 固液分離構造体
US20060226086A1 (en) * 2005-04-08 2006-10-12 Robinson Thomas C Centrifuge for blood processing systems
US7473216B2 (en) * 2005-04-21 2009-01-06 Fresenius Hemocare Deutschland Gmbh Apparatus for separation of a fluid with a separation channel having a mixer component
WO2007139551A1 (fr) * 2006-05-30 2007-12-06 Cytori Therapeutics, Inc. Systemes et procedes de manipulation de cellules regeneratives pour tissus adipeux
CN101086504B (zh) * 2006-06-06 2011-04-20 北京大学 一种微流体离心芯片及其加工方法
WO2008013863A2 (fr) * 2006-07-26 2008-01-31 Cytori Therapeutics, Inc. Génération d'un tissu adipeux et d'adipocytes
US20080200859A1 (en) * 2007-02-15 2008-08-21 Mehdi Hatamian Apheresis systems & methods
WO2009111461A2 (fr) * 2008-03-04 2009-09-11 University Of Utah Research Foundation Cellule d'écoulement microfluidique
WO2009113247A1 (fr) * 2008-03-12 2009-09-17 山科精器株式会社 Séparateur centrifuge
WO2010021993A1 (fr) 2008-08-19 2010-02-25 Cytori Therapeutics, Inc. Procédés d'utilisation de cellules issues du tissu adipeux dans le traitement du système lymphatique et d'une maladie maligne
KR101644534B1 (ko) * 2009-05-01 2016-08-01 비미니 테크놀로지스 엘엘씨 조직 및 세포 부유화 이식편의 최적화 시스템, 방법 및 조성물
US11285494B2 (en) 2009-08-25 2022-03-29 Nanoshell Company, Llc Method and apparatus for continuous removal of sub-micron sized particles in a closed loop liquid flow system
EP2470282A4 (fr) 2009-08-25 2015-07-29 Agnes Ostafin Procédé et appareil permettant une élimination en continu des particules submicroniques présentes dans un système d'écoulement de liquide en circuit fermé
US10099227B2 (en) 2009-08-25 2018-10-16 Nanoshell Company, Llc Method and apparatus for continuous removal of sub-micron sized particles in a closed loop liquid flow system
US10751464B2 (en) 2009-08-25 2020-08-25 Nanoshell Company, Llc Therapeutic retrieval of targets in biological fluids
WO2012139517A1 (fr) * 2011-04-13 2012-10-18 深圳华大基因科技有限公司 Dispositif microfluidique et utilisation de celui-ci
CN103191838B (zh) * 2012-01-09 2014-05-28 金卫医疗科技(上海)有限公司 一种用于血浆连续分离的曲面体容器
CN103191479B (zh) * 2012-01-09 2015-04-01 金卫医疗科技(上海)有限公司 一种血液在曲面体容器内连续离心分离的优化方法
CN103191837B (zh) * 2012-01-09 2014-05-21 金卫医疗科技(上海)有限公司 一种用于血液连续离心分离的分离盘结构
CN103191480B (zh) * 2012-01-09 2015-05-20 金卫医疗科技(上海)有限公司 一种血液连续离心分离时提高血浆提取纯度的方法
US9327296B2 (en) 2012-01-27 2016-05-03 Fenwal, Inc. Fluid separation chambers for fluid processing systems
WO2014160781A1 (fr) 2013-03-26 2014-10-02 Alliance Partners Llc Ensemble de concentration de fluides biologiques
MX2016003127A (es) 2013-09-19 2016-10-28 Cytori Therapeutics Inc Metodos de uso de células derivadas de tejido adiposo en la modulación de dolor y/o fibrosis.
DE102014000971A1 (de) 2014-01-25 2015-07-30 Fresenius Medical Care Deutschland Gmbh Vorrichtung zur Trennung von Blut in seine Bestandteile sowie Verfahren hierzu und Verwendung einer solchen Vorrichtung
US10207044B2 (en) 2015-07-29 2019-02-19 Fenwal, Inc. Five-port blood separation chamber and methods of using the same
EP3509663B1 (fr) 2016-09-06 2024-07-03 Fresenius Kabi Deutschland GmbH Procédé automatisé et appareil pour la collecte de leucocytes à partir de sang total
WO2020174005A1 (fr) 2019-02-28 2020-09-03 The Regenerative Group Ltd. Combinaison régénérative de plasma et de tissu adipeux

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE332906B (fr) * 1969-08-11 1971-02-22 Aga Ab
US3698626A (en) * 1971-05-17 1972-10-17 Atomic Energy Commission Centrifuge separator
US4934995A (en) * 1977-08-12 1990-06-19 Baxter International Inc. Blood component centrifuge having collapsible inner liner
US4007871A (en) * 1975-11-13 1977-02-15 International Business Machines Corporation Centrifuge fluid container
US4010894A (en) * 1975-11-21 1977-03-08 International Business Machines Corporation Centrifuge fluid container
US4430072A (en) * 1977-06-03 1984-02-07 International Business Machines Corporation Centrifuge assembly
US4356958A (en) * 1977-07-19 1982-11-02 The United States Of America As Represented By The Secretary Of Health And Human Services Blood cell separator
SE408859B (sv) * 1977-08-03 1979-07-16 Separex Sa Anordning foer astadkommande av obegraensad likriktad relativ rotation mellan aendarna av ett tradformigt element
US5006103A (en) * 1977-08-12 1991-04-09 Baxter International Inc. Disposable container for a centrifuge
US4387848A (en) * 1977-10-03 1983-06-14 International Business Machines Corporation Centrifuge assembly
US4386730A (en) * 1978-07-21 1983-06-07 International Business Machines Corporation Centrifuge assembly
SE412528B (sv) * 1978-07-25 1980-03-10 Separex Sa Centrifugrotor och kollaberbar separationsbehallare
SE7908036L (sv) * 1979-09-28 1981-03-29 Gambro Dialysatoren Separationsenhet for separering av vetskor, speciellt helblod
DE2948177A1 (de) * 1979-11-30 1981-06-04 Dr. Eduard Fresenius Chemisch-Pharmazeutische Industrie Kg Apparatebau Kg, 6380 Bad Homburg Separator fuer eine ultrazentrifuge
JPS56127749U (fr) * 1980-02-29 1981-09-29
US4402680A (en) * 1981-07-09 1983-09-06 Haemonetics Corporation Apparatus and method for separating fluid into components thereof
US4447221A (en) * 1982-06-15 1984-05-08 International Business Machines Corporation Continuous flow centrifuge assembly
US4439178A (en) * 1982-12-30 1984-03-27 International Business Machines Corporation Sealless centrifuge processing channel and tube system
US4479790A (en) * 1983-04-22 1984-10-30 Texasgulf, Inc. Centrifugal separator and method of operating same
US4647279A (en) * 1985-10-18 1987-03-03 Cobe Laboratories, Inc. Centrifugal separator
US4708712A (en) * 1986-03-28 1987-11-24 Cobe Laboratories, Inc. Continuous-loop centrifugal separator
SE459791B (sv) * 1986-05-16 1989-08-07 Omega Medicinteknik Ab Ringcentrifug
SE454413B (sv) * 1986-09-12 1988-05-02 Alfa Laval Separation Ab Centrifugalseparator med en rotor, vari ett bojligt organ strecker sig ett stycke lengs rotorns omkrets
DE3632500A1 (de) * 1986-09-24 1988-04-07 Fresenius Ag Zentrifugenanordnung
DE4226974C2 (de) * 1992-08-14 1994-08-11 Fresenius Ag Verfahren und Vorrichtung zur kontinuierlichen Aufbereitung einer Zellsuspension
US5904645A (en) * 1996-05-15 1999-05-18 Cobe Laboratories Apparatus for reducing turbulence in fluid flow

Also Published As

Publication number Publication date
JP2000093506A (ja) 2000-04-04
JP4027540B2 (ja) 2007-12-26
DE19841835A1 (de) 2000-03-23
DE19841835C2 (de) 2003-05-28
DE59912818D1 (de) 2005-12-29
US6277060B1 (en) 2001-08-21
ES2248948T3 (es) 2006-03-16
EP0985453A1 (fr) 2000-03-15

Similar Documents

Publication Publication Date Title
EP0985453B1 (fr) Chambre de centrifugation pour un séparateur des cellules
DE60036906T2 (de) Zentrifugale trennvorrichtung und verfahren zur trennung von flüssigkeitsbestandteilen
DE3710217C2 (de) Einrichtung für eine Zentrifuge
EP1459773B1 (fr) Dispositif de séparation à microstructure et méthode pour séparer des composants liquides d'un liquide contenant des particules
DE69634860T2 (de) Vorrichtung und Verfahren zur Teilchentrennung
DE69836648T2 (de) Vorrichtung und Verfahren zur Trennung von Flüssigkeitsbestandteilen
DE69310342T2 (de) Systeme und methoden zur ansammlung von plättchen mit verbesserter ausbeute
EP0583691B1 (fr) Procédé et dispositif pour le traitement continu d'une suspension cellulaire
DE2835307C2 (fr)
DE3410286A1 (de) Verfahren zur trennung von blut sowie vorrichtung zur durchfuehrung des verfahrens
DE3635300A1 (de) Zentrifugal-separator
DE3873532T2 (de) Vorrichtung zur separation von fluessigkeit.
EP0934031B1 (fr) Procede d'exploitation d'une unite de centrifugation du sang, et unite de centrifugation du sang pour mettre en oeuvre ce procede
DE2612696A1 (de) Vollmantel-dekantierzentrifuge
DE2130633A1 (de) Vollmantelschneckenzentrifuge
DE68910928T2 (de) Verfahren und Vorrichtung zur zentrifugalen Behandlung von Flüssigkeiten.
DE3853298T2 (de) Zentrifugaltrenner.
DE3826454C2 (fr)
DE2737759A1 (de) Zentrifuge
DE3426479C1 (de) Zentrifuge zum Klaeren oder Trennen von Suspensionen
EP1499416B1 (fr) Dispositif de filtration a systeme de separation par centrifugation integre
DE2925010A1 (de) Zentrifugenanordnung
DE2545754A1 (de) Schleudertrommel mit ablassventilen
DE69305494T2 (de) Sammelsysteme mit verbesserter ausbeute und methoden zur erhaltung von konzentrierten plättchen aus plättchenreichem plasma
DE69310711T2 (de) Kompakte blutverarbeitungssysteme mit verbesserter ausbeute

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): DE ES FR GB IT

AX Request for extension of the european patent

Free format text: AL;LT;LV;MK;RO;SI

17P Request for examination filed

Effective date: 20000824

AKX Designation fees paid

Free format text: DE ES FR GB IT

17Q First examination report despatched

Effective date: 20020813

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): DE ES FR GB IT

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REF Corresponds to:

Ref document number: 59912818

Country of ref document: DE

Date of ref document: 20051229

Kind code of ref document: P

GBT Gb: translation of ep patent filed (gb section 77(6)(a)/1977)

Effective date: 20060104

REG Reference to a national code

Ref country code: ES

Ref legal event code: FG2A

Ref document number: 2248948

Country of ref document: ES

Kind code of ref document: T3

ET Fr: translation filed
PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20060824

REG Reference to a national code

Ref country code: DE

Ref legal event code: R082

Ref document number: 59912818

Country of ref document: DE

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: GB

Payment date: 20140929

Year of fee payment: 16

Ref country code: FR

Payment date: 20140917

Year of fee payment: 16

Ref country code: ES

Payment date: 20140926

Year of fee payment: 16

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20140924

Year of fee payment: 16

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20140929

Year of fee payment: 16

REG Reference to a national code

Ref country code: DE

Ref legal event code: R119

Ref document number: 59912818

Country of ref document: DE

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: IT

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150904

GBPC Gb: european patent ceased through non-payment of renewal fee

Effective date: 20150904

REG Reference to a national code

Ref country code: FR

Ref legal event code: ST

Effective date: 20160531

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: GB

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150904

Ref country code: DE

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20160401

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: FR

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150930

REG Reference to a national code

Ref country code: ES

Ref legal event code: FD2A

Effective date: 20170203

PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

Ref country code: ES

Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

Effective date: 20150905