EP0915659A1 - Utilisation d'un enzyme ramifiant en boulangerie - Google Patents

Utilisation d'un enzyme ramifiant en boulangerie

Info

Publication number
EP0915659A1
EP0915659A1 EP97920609A EP97920609A EP0915659A1 EP 0915659 A1 EP0915659 A1 EP 0915659A1 EP 97920609 A EP97920609 A EP 97920609A EP 97920609 A EP97920609 A EP 97920609A EP 0915659 A1 EP0915659 A1 EP 0915659A1
Authority
EP
European Patent Office
Prior art keywords
dough
improving
enzyme
bread
branching enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP97920609A
Other languages
German (de)
English (en)
Inventor
Tina Spendler
Ole Bill Jorgensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novo Nordisk AS
Original Assignee
Novo Nordisk AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk AS filed Critical Novo Nordisk AS
Publication of EP0915659A1 publication Critical patent/EP0915659A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes

Definitions

  • the present invention relates to a bread-improving or dough-improving composition
  • a bread-improving or dough-improving composition comprising a branching enzyme as well as to a method of preparing a dough and/or a baked product by use of the composition and/or the enzyme.
  • Pentosanases such as xylanases, have gained considerable importance for use in the preparation of bread and baked products; in particular, to increase the volume and improve the anti-staling potential of bread and other baked products.
  • EP 396 162, EP 493 850 and EP 487 122 relate to bread improvers, deep-frozen dough and a fat-free pastry mix, respectively, comprising xylanase optionally in combina ⁇ tion with other enzymes.
  • WO 91/18977 discloses a method of preparing a pentosanase-containing preparation having increased baking activity.
  • EP 687 414 discloses the use of a cyclodextrin glucano- transferase to obtain increased loaf volume and improved flavour. Much attention has also focused on developing methods for the preparation of bread which is capable of staying fresh for a longer period of time and thus exhibits an increased resistance to staling. Various starch-modifying enzymes have been suggested for use as anti-staling agents.
  • thermostable ⁇ -amylases are described as useful in providing an antistaling effect to baked products
  • EP 412 607 describes the use of a thermostable ⁇ -1, 4- exoglucanase or ⁇ -1, 6-endoglucanase (e.g., pullulanase, amyloglucosidases or ⁇ -amylases) as an antistaling agent, and
  • EP 494 233 describes the use of a thermostable maltogenic ⁇ - amylase as an antistaling agent.
  • Branching enzyme (or 1,4- ⁇ -glucan branching enzyme, EC.2.4.1.18) is a transferase which is involved in the formation of ⁇ -1, 6-branches of starch and similar glucans.
  • the enzyme has been isolated from a number of plant and microbial sources and several cloned branching enzymes have been described. More specifically, Zevenhuizen (1964, Biochim. Biophys. Acta 81, 608-611) discloses a branching enzyme isolated from Arthrobacter globiformi s; US 4,454,161, a branching enzyme from Bacill us megateri ⁇ m; Walker and Builder (1977, Eur. J. Biochem.
  • coli branching enzyme Kiel, J.A.K.W., et al., (1992, J. DNA Sequencing and mapping, 3, 221-232) , a gene encoding a Bacill us caldolyti cus branching enzyme; Takata, H., et al. (1994, Appl. Environm. Microbiol. 60, 3096-3104) a gene encoding a Bacill us stearothermophil us branching enzyme; and Kiel, et al . (1989, Gene 78, 9-17), a gene encoding a branching enzyme from a Synechococcus species.
  • GB 2 095 681 discloses a process for the production of a branching enzyme from Bacill us and a method of preparing an improved food product by use of such an enzyme. It is stated that the enzyme may be useful in improving the shelf life of certain food products, including bread.
  • EP 418 945 discloses a thermostable branching enzyme isolated and cloned from Bacill us stearothermophil us . The enzyme is stated to be useful for modification of starch-like materials such as starch, amylose, amylopectin, dextrin, and other polyglucose materials, by introduction of additional branches into said materials. It is stated that the enzyme may be used for the production of food or feed products containing the modified starch-like materials, but no advantages of such use are described or indicated. It is the object of the present invention to provide a novel approach for achieving enzymatic improvements in dough quality and/or the baked product prepared therefrom.
  • the present invention rela ⁇ tes to a bread-improving and/or a dough-improving composition comprising an effective amount of a branching enzyme.
  • the terms "bread-improving composi- tion” and “dough-improving composition” are intended to indi ⁇ cate compositions which, in addition to the enzyme component, may comprise other substances conventionally used in baking to improve the properties of dough and/or baked products. Examples of such components are given below.
  • the term "an effective amount” is intended to indicate an amount of enzyme which is sufficient for providing a measurable effect on the parameter of interest. For example, it is an amount resulting in a detectable change of at least one of the properties improved according to the present invention; in particular, at least one of the properties believed to contribute to staling ( vi de the first paragraph of the section Detailed Description of the Invention below) .
  • the present invention relates to a method of preventing or reducing staling of a baked product, which method comprises, in the dough making process, to add an effective amount of a branching enzyme to the dough or dough ingredients and subject the resulting dough to baking under suitable conditions.
  • a branching enzyme for adding an effective amount of a branching enzyme to the dough or dough ingredients and subject the resulting dough to baking under suitable conditions.
  • the properties of dough and/or a baked product prepared from dough said to be improved by the method of the invention includes any property which may be improved by the action of the branching enzyme.
  • Important examples are an increased volume, an improved freshness (in terms of antistaling) and an improved structure and softness, as well as improved organoleptic qualities, of the baked product.
  • Also important is increased dough stability (i.e., a less sticky dough), thereby leading to improved machinability of the dough.
  • the improved machinability is of particular importance in con ⁇ nection with dough which is to be processed industrially.
  • the improved properties may, of course, be evaluated by comparison with dough and/or baked products prepared without addition of DGTase in accordance with the present invention.
  • the present invention relates to a dough and a baked product, respectively, produced by the present method as well as to a pre-mix comprising an effective amount of a branching enzyme as defined herein or a bread-improving or dough-improving composition of the invention.
  • pre-mix is intended to be understood in its conventional meaning, i.e. as a mix of baking agents, normally including flour, which may be used not only in industrial bread-baking plants/facilities, but also in retail bakeries.
  • the present invention relates to the use of a branching enzyme as defined herein to improve one or more properties of dough and/or a baked product prepared from dough, including (1) preventing or reducing the staling of bake . d products, in particular, bread; (2) increasing the loaf volume of a baked product; and (3) improving the organoleptic qualities of a baked product.
  • the branching enzyme to be used in the present invention is active at a pH in the range of 3 to 7, preferably with a pH optima in the range of 5 to 7.
  • the enzyme may be active during the dough preparation and/or the baking proces and it is presently preferred that the enzyme is active during both process steps.
  • the enzyme has an optimum activity above 45°C, more preferably above 55°C such as in the range of 45-90°C, and more preferably 55-90°C. It is preferred that the enzyme is inactivated during the baking process.
  • the branching enzyme has a substantial oxidation stability since a number of oxidation agents are present in the dough.
  • the origin of the branching enzyme to be used in the present invention is not critical as long as the enzyme in question has the properties mentioned above.
  • the branching enzyme may be of any origin, including mammalian, plant and microbial (including bacterial or fungal) origin.
  • Specific examples of branching enzymes contemplated for use in the present invention are the branching enzymes referred to above in the "Background of the Invention" section, in particular the enzyme described in EP 418 945.
  • the branching enzyme may be obtained from the organism in question by use of any suitable technique, and in particular by use of recombinant DNA techniques as known in the art.
  • the use of recombinant DNA techniques normally comprises cultivation of a host cell transformed with a recombinant DNA vector capable of expressing and carrying a DNA sequence encoding the enzyme in question, in a culture medium under conditions permitting the expression of the enzyme and recov ⁇ ering the enzyme from the culture.
  • the DNA sequence may be of genomic, cDNA or synthetic origin or any mixture of these, and may be isolated or synthesised in accordance with methods known in the art.
  • the enzyme may also be extracted from the organism or relevant part thereof by which it is produced in nature.
  • the bread- and/or dough-improving composition of the invention may comprise one or more additional enzymes.
  • additional enzyme (s) may be added together with the branching enzyme in the methods of the invention.
  • additional enzymes are a cellulase; a glycosyltransferase, in particular, 4- ⁇ -glucanotransferase (also known as DGTase) ; a hemicellulase, e.g., a pentosanase such as xylanase (useful for the partial hydrolysis of pentosans which increases the extensibility of the dough) ; a lipase (useful for the modification of lipids present in the dough or dough consti- tuents so as to soften the dough) ; an oxidase, e.g.
  • a glucose oxidase a peroxidase (useful for improving the dough consistency) ; a protease (useful for gluten weakening, in particular when using hard wheat flour) ; a peptidase, a transglutaminase and/or an amylololytic enzyme, in particular an amylolytic enzyme without any ⁇ -1, 4-endo-activity such as an ⁇ -1, 4-exoglucanase or and ⁇ -1, 6-endoglucanase, e.g.
  • the other enzyme components may be of any origin, including mammalian and plant, and preferably of microbial
  • the enzymes may be obtained by conventional techniques used in the art as mentioned above.
  • a specific example of a DGTase contemplated to be of use in the present invention is the potato DGTase described by Takaha et al . , J. Biol. Chem. vol. 268, 1391-1396, 1993. Said reference also discloses the recombinant production of the enzyme in an Eschericia coli and a Bacill us subtilis host cell.
  • the maltogenic amylase commercially available from Novo Nordisk A/S as Novamyl®
  • the antistaling agents Stalingase (TM) available from Gist- brocades N.V.
  • products of the product line Veron (TM) available from Rohm GmbH
  • the glucose oxidase available from Novo Nordisk A/S as Gluzyme®
  • the lipase available from Novo Nordisk A/S as Novozym® 677.
  • Transglutaminase may be used as described in EP 492 406.
  • the enzyme (s) to be used in the present invention may be in any form suited for the use in question, e.g. in the form of a dry powder or granulate, in particular a non-dusting granulate, a liquid, in particular a stabilised liquid, or a protected enzyme.
  • Granulates may be produced, e.g. as dis ⁇ closed in US 4,106,991 and US 4,661,452 (both to Novo Indus- tri A/S) , and may optionally be coated by methods known in the art.
  • Liquid enzyme preparations may, for instance, be stabilised by adding nutritionally acceptable stabilisers such as a sugar, a sugar alcohol or another polyol, lactic acid or another organic acid according to established methods.
  • Protected enzymes may be prepared according to the method disclosed in EP 238,216. Normally, for inclusion in pre-mixes or flour it is advan ⁇ tageous that the enzyme (s) is/are in the form of a dry product, e.g. a non-dusting granulate, whereas for inclusion together with a liquid it is advantageously in a liquid form.
  • the dough-improving and/or bread-improving composition may comprise a conventionally used baking agent, e.g.
  • a milk powder to provide crust colour
  • gluten to improve the gas retention power of weak flours
  • an emulsifier to improve dough extensibility and to some extent the consistency of the resulting bread
  • granulated fat for dough softening and consistency of bread
  • an oxidant e.g. ascorbic acid, potassium bromate, potassium iodate or ammonium persulfate; to strengthen the gluten structure
  • an amino acid e.g. cysteine
  • a sugar and salt (e.g. sodium chloride, calcium acetate, sodium sulfate or calcium sulphate; to make the dough firmer)
  • salt e.g. sodium chloride, calcium acetate, sodium sulfate or calcium sulphate; to make the dough firmer
  • flour or starch Such components may also be added directly to the dough in accordance with a method of the invention.
  • Suitable emulsifiers are mono- or digly- cerides, diacetyl tartaric acid esters of mono- or digly- cerides, sugar esters of fatty acids, polyglycerol esters of fatty acids, lactic acid esters of monoglycerides, acetic acid esters of monoglycerides, polyoxyethylene stearates, phospholipids and lecithin.
  • the bread-improving and/or dough improving composition of the invention is typically included in the dough in an amount corresponding to 0.01-5%, in particular 0.1-3%.
  • the enzyme (s) may be added as such to the mixture from which the dough is made or to any ingredient, e.g. flour, from which the dough is to be made.
  • the enzyme (s) may be added as a constituent of a dough-improving and/or a bread-improving composition as described above, either to flour or other dough ingredients or directly to the mixture from which the dough is to be made.
  • the dosage of the enzyme (s) to be used in the method of the present invention should be adapted to the nature and composition of the dough in question as well as to the nature of the enzyme (s) to be used.
  • the enzyme preparation is added in an amount corresponding to 0.01-1000 mg enzyme protein per kg of flour, preferably 0.1-100 mg enzyme protein per kg of flour, more preferably 0.1-10 mg enzyme protein per kg of flour.
  • the appropriate dosage of a given branching enzyme, optionally in combination with other enzyme (s), for exerting a desirable improvement of flour or crust colour of a baked product will depend on the enzyme (s) and the enzyme substrate (s) in question. The skilled person may determine a suitable enzyme unity dosage on the basis of methods known in the art.
  • these activities may be added separately or together with the branching enzyme, optionally as constituent (s) of the bread- improving and/or dough-improving composition of the invention.
  • the other enzyme activities may be any of the above described enzymes and may be dosed in accordance with established baking practice.
  • the branching enzyme optionally in combination with other enzyme (s) as described above, is added to any mixture of dough ingredients, to the dough, or to any of the ingredients to be included in the dough, in other words the enzyme (s) may be added in any step of the dough preparation and may be added in one, two or more steps, where appropriate.
  • the handling of the dough and/or baking is performed in any suitable manner for the dough and/or baked product in question, typically including the steps of kneading the dough, subjecting the dough to one or more proofing treat ⁇ ments, and baking the product under suitable conditions, i.e. at a suitable temperature and for a sufficient period of time.
  • the dough may be prepared by using a normal straight dough process, a sour dough process, an overnight dough method, a low-temperature and long-time fermentation method, a frozen dough method, the Chorleywood Bread process, or the Sponge and Dough process.
  • the dough and/or baked product prepared by the method of the invention are normally based on wheat meal or flour, optionally in combination with other types of meal or flour such as corn flour, rye meal, rye flour, oat flour or meal, soy flour, sorghum meal or flour, or potato meal or flour.
  • the term "baked product” is intended to include any product prepared from dough, either of a soft or a crisp character.
  • Examples of baked products, whether of a white, light or dark type, which may be advantageously produced by the present invention are bread (in particular white, whole-meal or rye bread) , typically in the form of loaves or rolls, French baguette-type bread, pita bread, tacos, cakes, pan-cakes, biscuits, crisp bread and the like.
  • the dough of the invention may be of any of the types dis ⁇ cussed above, and may be fresh, frozen or pre-baked.
  • the preparation of frozen dough is described by K. Kulp and K. Lorenz in "Frozen and Refrigerated Doughs and Batters". From the above disclosure, it will be apparent that the dough of the invention is normally a leavened dough or a dough to be subjected to leavening.
  • the dough may be leavened in various ways such as by adding sodium bicarbonate or the like, or by adding a leaven (fermenting dough), but it is preferred to leaven the dough by adding a suitable yeast culture such as a culture of Saccharomyces cerevi siae (baker's yeast) . Any of the commercially available S . cereviciae strains may be employed.
  • the present invention further relates to a pre-mix, e.g., in the form of a flour composition, for dough and or baked products made from dough, which pre-mix comprises a branching enzyme and optionally other enzymes as specified above.
  • the pre-mix may be prepared by mixing enzyme preparation (s) comprising the relevant enzyme (s) or a bread- improving and/or dough-improving composition of the invention comprising the enzyme (s) with a suitable carrier such as flour, starch, a sugar or a salt.
  • the pre-mix may contain other dough-improving and/or bread-improving additives, e.g., any of the additives, including enzymes, mentioned above. Techniques which can be used to determine improvements achieved by use of the present invention are described below.
  • the organoleptic qualities mentioned above may be evaluated using procedures well-established in the baking industry, and may include, for example, the use of a panel of trained taste-testers.
  • the branching enzyme activity may be determined as described in EP 418 945.
  • White bread may be prepared from the following basic recipe:
  • Dough and Baked Products may be evaluated as follows:
  • Loaf specific volume the mean value of 4 loaves volume are measured using the traditional rape seed method.
  • the specific volume is calculated as volume ml per g bread.
  • the specific volume of the control (without enzyme) is defined as 100.
  • the relative specific volume index is calculated as:
  • the dough stickiness and crumb structure may be evaluated visually according to the following scale:
  • a slice of bread is compressed with a constant speed in a texture analyzer, measuring the force for compression in g.
  • the softness of the crumb is measured as the force at 25% compression. 3. The force at 40% compression (P2) and after keeping

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Microbiology (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Seal Device For Vehicle (AREA)

Abstract

Composition permettant d'améliorer la qualité du pain ou de la pâte à pain et comprenant une quantité efficace d'un enzyme ramifiant, qui peut être mélangé, en option, à d'autres enzymes; utilisation de cette composition dans la fabrication de la pâte à pain et de produits de boulangerie.
EP97920609A 1996-05-02 1997-05-02 Utilisation d'un enzyme ramifiant en boulangerie Withdrawn EP0915659A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DK53296 1996-05-02
DK53196 1996-05-02
DK53196 1996-05-02
DK53296 1996-05-02
PCT/DK1997/000202 WO1997041736A1 (fr) 1996-05-02 1997-05-02 Utilisation d'un enzyme ramifiant en boulangerie

Publications (1)

Publication Number Publication Date
EP0915659A1 true EP0915659A1 (fr) 1999-05-19

Family

ID=26064168

Family Applications (1)

Application Number Title Priority Date Filing Date
EP97920609A Withdrawn EP0915659A1 (fr) 1996-05-02 1997-05-02 Utilisation d'un enzyme ramifiant en boulangerie

Country Status (6)

Country Link
EP (1) EP0915659A1 (fr)
JP (1) JP2000513568A (fr)
CN (1) CN1216902A (fr)
AU (1) AU2693297A (fr)
CA (1) CA2253283A1 (fr)
WO (1) WO1997041736A1 (fr)

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GB0211975D0 (en) * 2002-05-24 2002-07-03 Danisco Method
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
US6876932B1 (en) 1998-02-27 2005-04-05 Novozymes A/S Maltogenic alpha-amylase variants
JPH11243843A (ja) * 1998-02-27 1999-09-14 Ajinomoto Co Inc パン類の製造方法及びパン類製造用酵素製剤
US6608018B1 (en) * 1999-03-29 2003-08-19 Novozymes A/S Polypeptides having branching enzyme activity and nucleic acids encoding same
CA2444960C (fr) 2001-05-18 2011-08-09 Danisco A/S Procede servant a ameliorer la qualite d'une pate et d'un pain
EP1519653A1 (fr) * 2002-07-03 2005-04-06 Novozymes A/S Traitement d'une pate avec une lipoxygenase et une enzyme lipolytique
ES2215482B1 (es) * 2003-03-28 2005-12-16 Consejo Sup. De Invest. Cientificas Composicion mejoradora de masas para panaderia y pasteleria.
EP1614354A1 (fr) * 2004-07-05 2006-01-11 LESAFFRE et Cie Procedes et produits de panification
AU2005263954B2 (en) 2004-07-16 2011-04-07 Dupont Nutrition Biosciences Aps Enzymatic oil-degumming method
JP4862759B2 (ja) * 2006-06-30 2012-01-25 味の素株式会社 澱粉含有食品の製造方法及び澱粉含有食品改質用の酵素製剤
JP6622964B2 (ja) * 2013-01-24 2019-12-18 味の素株式会社 デンプン含有食品の製造方法及びデンプン含有食品改質用の酵素製剤
JP6555484B2 (ja) * 2014-03-31 2019-08-07 ナガセケムテックス株式会社 パンその他穀物粉膨化食品の凝集性改善剤
CN107105677A (zh) * 2015-01-16 2017-08-29 诺维信公司 用来改进烘焙品的可切片性的方法
JP6661116B2 (ja) * 2016-02-15 2020-03-11 奥野製薬工業株式会社 パン類品質改良剤およびそれを用いたパン類の製造方法
CN106538652A (zh) * 2016-10-21 2017-03-29 李郑松 一种抗老化面包及其制备方法
JP7275545B2 (ja) * 2017-11-29 2023-05-18 味の素株式会社 酵素を用いた澱粉含有食品の製造方法
JP7286959B2 (ja) * 2017-12-05 2023-06-06 味の素株式会社 酵素を用いた澱粉含有食品の製造方法
MX2020013319A (es) * 2018-06-12 2021-02-22 Novozymes As Menos azúcar añadido en productos horneados.
JP2020058318A (ja) * 2018-10-12 2020-04-16 物産フードサイエンス株式会社 ドウまたはドウ加熱食品における分枝酵素の新規用途
JP7360843B2 (ja) * 2019-08-16 2023-10-13 オリエンタル酵母工業株式会社 パン類用品質向上剤、パン類の製造方法およびパン類の品質向上方法
JP7435198B2 (ja) 2020-04-16 2024-02-21 日油株式会社 生パン粉用油脂組成物および生パン粉用生地、生パン粉用生地の製造方法
CN115316417B (zh) * 2022-08-23 2023-07-25 江南大学 一种改善无麸质面包加工品质的方法、无麸质面包

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DK474589D0 (da) * 1989-09-27 1989-09-27 Novo Nordisk As Fremgangsmaade til fremstilling af bageriprodukter
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Also Published As

Publication number Publication date
CN1216902A (zh) 1999-05-19
CA2253283A1 (fr) 1997-11-13
AU2693297A (en) 1997-11-26
WO1997041736A1 (fr) 1997-11-13
JP2000513568A (ja) 2000-10-17

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