EP0871770A1 - Sondes de peptides polymeres et leurs utilisations - Google Patents

Sondes de peptides polymeres et leurs utilisations

Info

Publication number
EP0871770A1
EP0871770A1 EP96916483A EP96916483A EP0871770A1 EP 0871770 A1 EP0871770 A1 EP 0871770A1 EP 96916483 A EP96916483 A EP 96916483A EP 96916483 A EP96916483 A EP 96916483A EP 0871770 A1 EP0871770 A1 EP 0871770A1
Authority
EP
European Patent Office
Prior art keywords
assay
test sample
signal
probe
rrna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP96916483A
Other languages
German (de)
English (en)
Inventor
Lynn Valdivia
Hong Yu
David A. Dunn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Abbott Laboratories
Original Assignee
Abbott Laboratories
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Laboratories filed Critical Abbott Laboratories
Publication of EP0871770A1 publication Critical patent/EP0871770A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Abstract

Méthode d'hybridation in situ servant à détecter de l'ARNr ou des gènes de résistance aux médicaments au moyen d'acides nucléiques de peptides (PNAs) ou de composés morpholino en tant que sondes. L'invention concerne également des trousses permettant de mettre ces méthodes en application.
EP96916483A 1995-05-18 1996-05-16 Sondes de peptides polymeres et leurs utilisations Withdrawn EP0871770A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US444143 1982-11-24
US44414395A 1995-05-18 1995-05-18
PCT/US1996/007075 WO1996036734A1 (fr) 1995-05-18 1996-05-16 Sondes de peptides polymeres et leurs utilisations

Publications (1)

Publication Number Publication Date
EP0871770A1 true EP0871770A1 (fr) 1998-10-21

Family

ID=23763680

Family Applications (1)

Application Number Title Priority Date Filing Date
EP96916483A Withdrawn EP0871770A1 (fr) 1995-05-18 1996-05-16 Sondes de peptides polymeres et leurs utilisations

Country Status (4)

Country Link
EP (1) EP0871770A1 (fr)
JP (1) JP2001502881A (fr)
CA (1) CA2221179A1 (fr)
WO (1) WO1996036734A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH10500310A (ja) * 1994-05-19 1998-01-13 ダコ アクティーゼルスカブ 淋菌及びトラコーマ クラミジアの検出のためのｐｎａ プローブ
US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
ATE243761T1 (de) * 1996-10-04 2003-07-15 Dako As Sonden zur detektion von mycobakterien
CA2218440A1 (fr) * 1996-12-21 1998-06-21 Boehringer Mannheim Gmbh Methode d'analyse avec amplification du signal
AU8890498A (en) * 1997-08-26 1999-03-16 Microscreen B.V. Rapid detection and identification of micro-organisms
US6300318B1 (en) * 1997-09-16 2001-10-09 Peter E. Nielsen Antibacterial and antibiotic methods using peptide nucleic acids and pharmaceutical compositions therefor
WO2000065092A2 (fr) * 1999-04-22 2000-11-02 Science And Technology Corporation Blocage de la fixation non specifique des granulocytes dans la detection de micro-organismes
WO2000065093A2 (fr) * 1999-04-22 2000-11-02 Science And Technology Corporation Procedes d'hybridation in situ permettant de reduire l'occurrence de faux resultats positifs et de cibler des micro-organismes multiples
AU2001286966B2 (en) * 2000-08-30 2007-03-01 Avi Biopharma, Inc. Method for analysis of oligonucleotide analogs
WO2011134474A1 (fr) * 2010-04-30 2011-11-03 Exiqon A/S Procédé et tampon d'hybridation in situ

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990010715A1 (fr) * 1989-03-07 1990-09-20 Molecular Biosystems, Inc. Hybridation in situ en suspension pour la detection ou la separation de cellules
US5539082A (en) * 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
WO1992022670A1 (fr) * 1991-06-12 1992-12-23 Genpharm International, Inc. Detection precoce d'embryons transgeniques
DE4216949C2 (de) * 1992-05-22 1997-07-24 Christoph Prof Dr Dr Cremer Nichtenzymatisches Verfahren zur In-situ-Hybridisierung bei spezifischen Proben
IL106273A0 (en) * 1992-07-17 1993-11-15 Res Dev Foundation Rapid detection of biopolymers in stained specimens
WO1994002642A1 (fr) * 1992-07-17 1994-02-03 Aprogenex, Inc. Composes reducteurs de fond pour essais fluorimetriques in-situ a mediation par sonde
AU7661794A (en) * 1993-09-21 1995-04-10 Amersham International Plc Reagents comprising chimeric molecules of nucleic acids and nucleic acid analogs
JPH10500310A (ja) * 1994-05-19 1998-01-13 ダコ アクティーゼルスカブ 淋菌及びトラコーマ クラミジアの検出のためのｐｎａ プローブ

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9636734A1 *

Also Published As

Publication number Publication date
CA2221179A1 (fr) 1996-11-21
JP2001502881A (ja) 2001-03-06
WO1996036734A1 (fr) 1996-11-21

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