WO1990010715A1 - Hybridation in situ en suspension pour la detection ou la separation de cellules - Google Patents

Hybridation in situ en suspension pour la detection ou la separation de cellules Download PDF

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Publication number
WO1990010715A1
WO1990010715A1 PCT/US1990/001191 US9001191W WO9010715A1 WO 1990010715 A1 WO1990010715 A1 WO 1990010715A1 US 9001191 W US9001191 W US 9001191W WO 9010715 A1 WO9010715 A1 WO 9010715A1
Authority
WO
WIPO (PCT)
Prior art keywords
probe
cells
cell
suspended
nucleic acid
Prior art date
Application number
PCT/US1990/001191
Other languages
English (en)
Inventor
Jo Anne H. Kerschner
Edward G. Jablonski
Original Assignee
Molecular Biosystems, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Molecular Biosystems, Inc. filed Critical Molecular Biosystems, Inc.
Publication of WO1990010715A1 publication Critical patent/WO1990010715A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

Abstract

Le procédé décrit permet de détecter des séquences d'acides nucléiques dans une cellule en suspension. Une technique pour l'hybridation directe de désoxyoligonucléotides marqués avec des séquences d'ADN ou d'ARN cellulaires contenues dans des cellules intactes en suspension ainsi que des techniques de séparation de cellules contenant l'ADN ou l'ARN hybridé des cellules qui ne contiennent pas d'ADN ou d'ARN hybridé sont décrites.
PCT/US1990/001191 1989-03-07 1990-03-05 Hybridation in situ en suspension pour la detection ou la separation de cellules WO1990010715A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31998289A 1989-03-07 1989-03-07
US319,982 1989-03-07

Publications (1)

Publication Number Publication Date
WO1990010715A1 true WO1990010715A1 (fr) 1990-09-20

Family

ID=23244366

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/001191 WO1990010715A1 (fr) 1989-03-07 1990-03-05 Hybridation in situ en suspension pour la detection ou la separation de cellules

Country Status (5)

Country Link
EP (1) EP0462221A1 (fr)
JP (1) JPH04505556A (fr)
AU (1) AU646635B2 (fr)
CA (1) CA2011571A1 (fr)
WO (1) WO1990010715A1 (fr)

Cited By (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019967A1 (fr) * 1991-05-03 1992-11-12 Ig Laboratories, Inc. Procede permettant d'obtenir des cellules porteuses d'informations
WO1993004197A1 (fr) * 1991-08-13 1993-03-04 Universitaire Instelling Antwerpen Procede pour detecter un virus dans la substance cellulaire d'un prelevement ou d'un liquide organique
WO1994002646A1 (fr) * 1992-07-17 1994-02-03 Aprogenex Inc. Enrichissement et identification de cellules foetales dans le sang maternel pour l'hybridation in situ
WO1995003428A1 (fr) * 1993-07-20 1995-02-02 University Of Massachusetts Medical Center Procede d'hybridation in vivo d'acide nucleique
WO1996004402A1 (fr) * 1994-08-05 1996-02-15 The Regents Of The University Of California Detection de mycobacteries
US5501952A (en) * 1992-07-17 1996-03-26 Aprogenex, Inc. Analogues of reporter groups as background reducers in hybridization assays
US5521061A (en) * 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies
WO1996036734A1 (fr) * 1995-05-18 1996-11-21 Abbott Laboratories Sondes de peptides polymeres et leurs utilisations
US5582982A (en) * 1992-07-17 1996-12-10 Aprogenex, Inc. Background-reducing compounds for probe-mediated fluorimetric flow cytometric assays
US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
US5985563A (en) * 1994-05-19 1999-11-16 Dako A/S Detection of Ribosomal RNA using PNA probes
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6610475B1 (en) 1993-02-10 2003-08-26 Gen-Probe Incorporated Kits for processing biological specimens for nucleic acid hybridization assays, target amplification reactions and similar uses
WO2003091449A1 (fr) * 2002-04-26 2003-11-06 Riken Methode de mesure de l'activite de la neprilysine
EP1461453A2 (fr) * 2001-11-30 2004-09-29 Amnis Corporation Procedes d'identification de molecules cibles cellulaires
WO2006012302A2 (fr) * 2004-06-28 2006-02-02 Exagen Diagnostics, Inc. Methodes d'hybridation d'arn in situ a fluorescence
US7925069B2 (en) 1999-01-25 2011-04-12 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US8548219B2 (en) 1999-01-25 2013-10-01 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells
US9528989B2 (en) 2004-03-16 2016-12-27 Amnis Corporation Image-based quantitation of molecular translocation
CN107748256A (zh) * 2017-10-11 2018-03-02 上海医盈网络科技有限公司 一种循环肿瘤细胞的液体活检检测方法
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
US11299764B2 (en) 2015-11-20 2022-04-12 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
US11506655B2 (en) 2016-07-29 2022-11-22 Streck, Inc. Suspension composition for hematology analysis control

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU636854B2 (en) * 1988-08-31 1993-05-13 Aprogenex, Inc. One-step in situ hybridization assay

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038143A (en) * 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
GB2139349A (en) * 1983-04-29 1984-11-07 Nat Res Dev Method of determining nucleotide sequences in cells and of isolating nucleic acids from cells
WO1985004720A1 (fr) * 1984-04-05 1985-10-24 Howard Florey Institute Of Experimental Physiology Hystochimie d'hybridisation

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU636854B2 (en) * 1988-08-31 1993-05-13 Aprogenex, Inc. One-step in situ hybridization assay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4038143A (en) * 1973-10-29 1977-07-26 The Regents Of The University Of Michigan Test kit for the genetic detection of microorganisms
GB2139349A (en) * 1983-04-29 1984-11-07 Nat Res Dev Method of determining nucleotide sequences in cells and of isolating nucleic acids from cells
EP0127327A1 (fr) * 1983-04-29 1984-12-05 National Research Development Corporation Méthode de détermination de la séquence des nucléotides dans des cellules et d'isolation des acides nucléiques des cellules
WO1985004720A1 (fr) * 1984-04-05 1985-10-24 Howard Florey Institute Of Experimental Physiology Hystochimie d'hybridisation

Cited By (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992019967A1 (fr) * 1991-05-03 1992-11-12 Ig Laboratories, Inc. Procede permettant d'obtenir des cellules porteuses d'informations
WO1993004197A1 (fr) * 1991-08-13 1993-03-04 Universitaire Instelling Antwerpen Procede pour detecter un virus dans la substance cellulaire d'un prelevement ou d'un liquide organique
BE1005174A3 (nl) * 1991-08-13 1993-05-11 Universitaire Instelling Antwe Werkwijze voor het opsporen van een virus in celmateriaal van een afschraapsel of lichaamsvocht.
US5501952A (en) * 1992-07-17 1996-03-26 Aprogenex, Inc. Analogues of reporter groups as background reducers in hybridization assays
US5521061A (en) * 1992-07-17 1996-05-28 Aprogenex, Inc. Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies
US5582982A (en) * 1992-07-17 1996-12-10 Aprogenex, Inc. Background-reducing compounds for probe-mediated fluorimetric flow cytometric assays
WO1994002646A1 (fr) * 1992-07-17 1994-02-03 Aprogenex Inc. Enrichissement et identification de cellules foetales dans le sang maternel pour l'hybridation in situ
US6610475B1 (en) 1993-02-10 2003-08-26 Gen-Probe Incorporated Kits for processing biological specimens for nucleic acid hybridization assays, target amplification reactions and similar uses
WO1995003428A1 (fr) * 1993-07-20 1995-02-02 University Of Massachusetts Medical Center Procede d'hybridation in vivo d'acide nucleique
US5728527A (en) * 1993-07-20 1998-03-17 University Of Massachusetts Medical Center Detection of hybridized oligonocleotide probes in living cells
US6169169B1 (en) 1994-05-19 2001-01-02 Dako A/S PNA probes for detection of Neisseria gonorrhoeae and Chlamydia trachomatis
US5985563A (en) * 1994-05-19 1999-11-16 Dako A/S Detection of Ribosomal RNA using PNA probes
WO1996004402A1 (fr) * 1994-08-05 1996-02-15 The Regents Of The University Of California Detection de mycobacteries
US5582985A (en) * 1994-08-05 1996-12-10 The Regents Of The University Of California Detection of mycobacteria
WO1996036734A1 (fr) * 1995-05-18 1996-11-21 Abbott Laboratories Sondes de peptides polymeres et leurs utilisations
US5888733A (en) * 1995-11-16 1999-03-30 Dako A/S In situ hybridization to detect specific nucleic acid sequences in eucaryotic samples
US8518710B2 (en) 1997-08-01 2013-08-27 Life Technologies Corporation Photon reducing agents for fluorescence assays
US7067324B2 (en) 1997-08-01 2006-06-27 Invitrogen Corporation Photon reducing agents for fluorescence assays
US6214563B1 (en) 1997-08-01 2001-04-10 Aurora Biosciences Corporation Photon reducing agents for reducing undesired light emission in assays
US6221612B1 (en) 1997-08-01 2001-04-24 Aurora Biosciences Corporation Photon reducing agents for use in fluorescence assays
US6200762B1 (en) 1997-08-01 2001-03-13 Aurora Biosciences Corporation Photon reducing agents and compositions for fluorescence assays
US8163562B2 (en) 1997-08-01 2012-04-24 Life Technologies Corporation Photon reducing agents for fluorescence assays
US7785536B2 (en) 1997-08-01 2010-08-31 Life Technologies Corporation Photon reducing agents for fluorescence assays
US8660332B2 (en) 1999-01-25 2014-02-25 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US8885913B2 (en) 1999-01-25 2014-11-11 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US8548219B2 (en) 1999-01-25 2013-10-01 Amnis Corporation Detection of circulating tumor cells using imaging flow cytometry
US7925069B2 (en) 1999-01-25 2011-04-12 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
US8406498B2 (en) 1999-01-25 2013-03-26 Amnis Corporation Blood and cell analysis using an imaging flow cytometer
EP1461453A2 (fr) * 2001-11-30 2004-09-29 Amnis Corporation Procedes d'identification de molecules cibles cellulaires
EP1461453A4 (fr) * 2001-11-30 2004-12-15 Amnis Corp Procedes d'identification de molecules cibles cellulaires
WO2003091449A1 (fr) * 2002-04-26 2003-11-06 Riken Methode de mesure de l'activite de la neprilysine
US7572574B2 (en) 2002-04-26 2009-08-11 Riken Method of measuring neprilysin activity
US8953866B2 (en) 2004-03-16 2015-02-10 Amnis Corporation Method for imaging and differential analysis of cells
US9528989B2 (en) 2004-03-16 2016-12-27 Amnis Corporation Image-based quantitation of molecular translocation
WO2006012302A2 (fr) * 2004-06-28 2006-02-02 Exagen Diagnostics, Inc. Methodes d'hybridation d'arn in situ a fluorescence
WO2006012302A3 (fr) * 2004-06-28 2006-05-04 Exagen Diagnostics Inc Methodes d'hybridation d'arn in situ a fluorescence
US11168351B2 (en) 2015-03-05 2021-11-09 Streck, Inc. Stabilization of nucleic acids in urine
US11299764B2 (en) 2015-11-20 2022-04-12 Streck, Inc. Single spin process for blood plasma separation and plasma composition including preservative
US11506655B2 (en) 2016-07-29 2022-11-22 Streck, Inc. Suspension composition for hematology analysis control
CN107748256A (zh) * 2017-10-11 2018-03-02 上海医盈网络科技有限公司 一种循环肿瘤细胞的液体活检检测方法

Also Published As

Publication number Publication date
AU5289990A (en) 1990-10-09
EP0462221A1 (fr) 1991-12-27
CA2011571A1 (fr) 1990-09-07
AU646635B2 (en) 1994-03-03
JPH04505556A (ja) 1992-10-01

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