EP0807467A3 - Device for automatically carrying out polymerase chain reactions - Google Patents

Device for automatically carrying out polymerase chain reactions Download PDF

Info

Publication number
EP0807467A3
EP0807467A3 EP97112702A EP97112702A EP0807467A3 EP 0807467 A3 EP0807467 A3 EP 0807467A3 EP 97112702 A EP97112702 A EP 97112702A EP 97112702 A EP97112702 A EP 97112702A EP 0807467 A3 EP0807467 A3 EP 0807467A3
Authority
EP
European Patent Office
Prior art keywords
der
carrier
und
eine
die
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP97112702A
Other languages
German (de)
French (fr)
Other versions
EP0807467B1 (en
EP0807467A2 (en
Inventor
Rolf Moser
Lukas Birrer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of EP0807467A2 publication Critical patent/EP0807467A2/en
Publication of EP0807467A3 publication Critical patent/EP0807467A3/en
Application granted granted Critical
Publication of EP0807467B1 publication Critical patent/EP0807467B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L9/00—Supporting devices; Holding devices
    • B01L9/06—Test-tube stands; Test-tube holders
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1079—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices with means for piercing stoppers or septums

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Enzymes And Modification Thereof (AREA)
  • General Induction Heating (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Testing Resistance To Weather, Investigating Materials By Mechanical Methods (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Control Of Combustion (AREA)
  • Investigating Or Analyzing Materials Using Thermal Means (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Control Of Temperature (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

Vorrichtung zur automatischen Durchführung von Polymerase-Kettenreaktionen in einer Vielzahl von Reaktionsbehältern, wobei jedes Reaktionsbehälter mit einem Deckel verschlossen ist und ein vorbestimmtes Volumen eines flüssigen Reaktionsgemisches enthält, welche Vorrichtung folgende Komponenten enthält:

  • a) einen Träger (33), der eine Anordnung von Kammern (27) zur Aufnahme der Reaktionsbehälter hat (21), wobei jede Kammer dazu geeignet ist, den unteren Teil eines Reaktionsbehälters aufzunehmen, und wobei der Träger (33) aus einem Material besteht, dass eine hohe thermische Leitfähigkeit hat, und dass er eine obere Fläche, eine untere Fläche und eine zylindrische Aussenwand hat, wobei jede der Kammer (27) des Trägers (33) eine Oeffnung hat, die in der oberen Fläche des Trägers liegt,
  • b) in den Kammern (27) des Trägers (33) angeordneten Reaktionsbehälter (21), die je mit einem Deckel (87) verschlossen sind,
  • c) eine computergesteuerte Steuer- und Regeleinrichtung, und
  • d) durch die Steuer- und Regeleinrichtung gesteuerte Mittel zur zyklischen Aenderung der Temperatur des Trägers.
    • Device for automatically carrying out polymerase chain reactions in a multiplicity of reaction containers, each reaction container being closed with a lid and containing a predetermined volume of a liquid reaction mixture, which device contains the following components:
  • a) a carrier (33) which has an arrangement of chambers (27) for receiving the reaction container (21), each chamber being suitable for receiving the lower part of a reaction container, and wherein the carrier (33) consists of a material that has a high thermal conductivity and that it has an upper surface, a lower surface and a cylindrical outer wall, each of the chamber (27) of the carrier (33) having an opening which lies in the upper surface of the carrier,
  • b) reaction vessels (21) arranged in the chambers (27) of the carrier (33), each of which is closed with a lid (87),
  • (c) a computerized control and regulation device, and
  • d) means controlled by the control and regulating device for cyclically changing the temperature of the carrier.

    • Zur Erleichterung der Entfernung der Reaktionsbehälter aus den Kammern des Trägers ist die Vorrichtung dadurch gekennzeichnet, dass

  • (i) die Anordnung der Kammer (27) im Träger (33) ringförmig ist,
  • (ii) sie einen klappbaren Deckel (28) zum Festhalten der verschlossenen, im Träger (33) angeordneten Reaktionsbehälter (21) enthält, und
  • (iii) eine Aushebevorrichtung zur Erleichterung der Entnahme der Reaktionsbehälter (21) aus den Kammern (27) im Träger (33) enthält, welche Aushebevorrichtung einen Auswurfhebel (55), der an einem Ende mit einem Scharnier des klappbaren Deckels (28) verbunden und am anderen Ende frei ist, und eine Auswurfscheibe (58) enthält, die mit der Rotationssymmetrieachse des Trägers (33) konzentrisch ist, auf dem Auswurfhebel (55) befestigt ist und an der Peripherie eine Anordnung von Ausnehmungen (61) hat, die zur Entnahme der Reaktionsbehälter (21) aus den Kammern (27) dienen.
    • Figure 00000001
    In order to facilitate the removal of the reaction containers from the chambers of the carrier, the device is characterized in that
  • (i) the arrangement of the chamber (27) in the carrier (33) is annular,
  • (ii) it contains a hinged lid (28) for holding the closed reaction containers (21) arranged in the carrier (33), and
  • (iii) a lifting device for facilitating the removal of the reaction containers (21) from the chambers (27) in the carrier (33), which lifting device comprises an ejection lever (55) which is connected at one end to a hinge of the hinged cover (28) and is free at the other end, and contains an ejection disc (58), which is concentric with the rotational symmetry axis of the carrier (33), is fastened on the ejection lever (55) and has an arrangement of recesses (61) on the periphery, which can be removed the reaction container (21) from the chambers (27) are used.
    • Figure 00000001
    EP97112702A 1993-09-10 1994-08-31 Device for automatically carrying out polymerase chain reactions Expired - Lifetime EP0807467B1 (en)

    Applications Claiming Priority (4)

    Application Number Priority Date Filing Date Title
    CH2717/93 1993-09-10
    CH271793 1993-09-10
    CH271793 1993-09-10
    EP94113574A EP0642831B1 (en) 1993-09-10 1994-08-31 Device and process for automatically carrying out temperature cycling

    Related Parent Applications (2)

    Application Number Title Priority Date Filing Date
    EP94113574.1 Division 1994-08-31
    EP94113574A Division EP0642831B1 (en) 1993-09-10 1994-08-31 Device and process for automatically carrying out temperature cycling

    Publications (3)

    Publication Number Publication Date
    EP0807467A2 EP0807467A2 (en) 1997-11-19
    EP0807467A3 true EP0807467A3 (en) 1998-06-03
    EP0807467B1 EP0807467B1 (en) 2001-12-19

    Family

    ID=4240017

    Family Applications (3)

    Application Number Title Priority Date Filing Date
    EP97112702A Expired - Lifetime EP0807467B1 (en) 1993-09-10 1994-08-31 Device for automatically carrying out polymerase chain reactions
    EP94113574A Expired - Lifetime EP0642831B1 (en) 1993-09-10 1994-08-31 Device and process for automatically carrying out temperature cycling
    EP97112710A Expired - Lifetime EP0807468B1 (en) 1993-09-10 1994-08-31 Device for automatically carrying out polymerase chain reactions

    Family Applications After (2)

    Application Number Title Priority Date Filing Date
    EP94113574A Expired - Lifetime EP0642831B1 (en) 1993-09-10 1994-08-31 Device and process for automatically carrying out temperature cycling
    EP97112710A Expired - Lifetime EP0807468B1 (en) 1993-09-10 1994-08-31 Device for automatically carrying out polymerase chain reactions

    Country Status (10)

    Country Link
    US (2) US5616301A (en)
    EP (3) EP0807467B1 (en)
    JP (1) JPH07151764A (en)
    AT (3) ATE211024T1 (en)
    CA (1) CA2130013C (en)
    DE (3) DE59410021D1 (en)
    DK (1) DK0642831T3 (en)
    ES (3) ES2155456T3 (en)
    GR (1) GR3035810T3 (en)
    PT (1) PT642831E (en)

    Families Citing this family (182)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    US5767259A (en) 1994-12-27 1998-06-16 Naxcor Oligonucleotides containing base-free linking groups with photoactivatable side chains
    US6495676B1 (en) 1993-04-13 2002-12-17 Naxcor Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents
    US6277570B1 (en) 1993-04-13 2001-08-21 Naxcor Nucleic acid sequence detection employing probes comprising non-nucleosidic coumarin derivatives as polynucleotide-crosslinking agents
    CA2130517C (en) * 1993-09-10 1999-10-05 Walter Fassbind Array of reaction containers for an apparatus for automatic performance of temperature cycles
    CA2130013C (en) * 1993-09-10 1999-03-30 Rolf Moser Apparatus for automatic performance of temperature cycles
    DE29505897U1 (en) * 1995-01-12 1995-08-24 Schulz, Joachim, Dipl.-Ing., 06484 Quedlinburg Thermal shaker
    DE19519015C1 (en) * 1995-05-24 1996-09-05 Inst Physikalische Hochtech Ev Miniaturised multi-chamber thermo-cycler for polymerase chain reaction
    DE19521947C1 (en) * 1995-06-16 1996-08-22 Medipro Medizinische Diagnosti Vibrating water bath assembly with at least two individually temp.-controlled positions
    DE19534632A1 (en) * 1995-09-19 1997-03-20 Boehringer Mannheim Gmbh System for temperature change treatment of sample liquids
    DE19540877C2 (en) * 1995-11-02 1998-02-26 Byk Sangtec Diagnostica Modular reagent cartridge
    DE29519602U1 (en) * 1995-12-11 1996-04-18 Reimann, Hans-Jürgen, Prof. Dr. Dr., 82067 Ebenhausen Mobile incubation device
    US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
    US6635492B2 (en) 1996-01-25 2003-10-21 Bjs Company Ltd. Heating specimen carriers
    US5882903A (en) * 1996-11-01 1999-03-16 Sarnoff Corporation Assay system and method for conducting assays
    DE19713993A1 (en) * 1996-11-22 1998-05-28 Joachim Dipl Ing Schulz Lightweight apparatus heats and stirs sample tubes containing for example, biological materials in microlitre quantities
    US6045755A (en) * 1997-03-10 2000-04-04 Trega Biosciences,, Inc. Apparatus and method for combinatorial chemistry synthesis
    US7133726B1 (en) 1997-03-28 2006-11-07 Applera Corporation Thermal cycler for PCR
    EP1127619B1 (en) * 1997-03-28 2003-10-08 PE Corporation (NY) Assembly for thermal cycler for PCR
    EP2913109A1 (en) * 1997-03-28 2015-09-02 Applera Corporation Assembly for thermal cycler for PCR
    AU9107298A (en) * 1997-08-20 1999-03-08 Biopore, Inc. Cassette device and system to facilitate cryopreservation
    US5942432A (en) * 1997-10-07 1999-08-24 The Perkin-Elmer Corporation Apparatus for a fluid impingement thermal cycler
    DE29720432U1 (en) 1997-11-19 1999-03-25 Heimberg, Wolfgang, Dr., 85560 Ebersberg robot
    US6251686B1 (en) * 1998-02-26 2001-06-26 Edward J. Studer Liquid transfer apparatus
    EP1614473A3 (en) 1998-05-01 2007-03-14 Gen-Probe Incorporated Multiple ring assembly for providing specimen to reaction receptacles within an automated analyzer
    US8337753B2 (en) 1998-05-01 2012-12-25 Gen-Probe Incorporated Temperature-controlled incubator having a receptacle mixing mechanism
    ATE271422T1 (en) * 1998-05-01 2004-08-15 Hoffmann La Roche DEVICE FOR THE SIMULTANEOUS MONITORING OF REACTIONS THAT TAKE PLACE IN A LOT OF REACTION VESSELS
    ES2229465T3 (en) 1998-05-04 2005-04-16 F. Hoffmann-La Roche Ag TREMO-VARIATOR THAT HAS A COVER THAT IS AUTOMATICALLY PLACED IN POSITION.
    US6780617B2 (en) * 2000-12-29 2004-08-24 Chen & Chen, Llc Sample processing device and method
    EP1005656B1 (en) 1998-06-24 2014-05-21 Chen & Chen, LLC Fluid sample testing system
    US7799521B2 (en) * 1998-06-24 2010-09-21 Chen & Chen, Llc Thermal cycling
    US6413780B1 (en) * 1998-10-14 2002-07-02 Abbott Laboratories Structure and method for performing a determination of an item of interest in a sample
    DE19859586C1 (en) * 1998-12-22 2000-07-13 Mwg Biotech Ag Thermal cycler device
    EP1045038A1 (en) * 1999-04-08 2000-10-18 Hans-Knöll-Institut Für Naturstoff-Forschung E.V. Rapid heat block thermocycler
    DE19926937A1 (en) * 1999-06-14 2001-01-04 Biopsytec Gmbh Holder for standard laboratory reaction tube with captive lid uses lid to assist fixing in holder
    US6472186B1 (en) 1999-06-24 2002-10-29 Andre Quintanar High speed process and apparatus for amplifying DNA
    US6657169B2 (en) * 1999-07-30 2003-12-02 Stratagene Apparatus for thermally cycling samples of biological material with substantial temperature uniformity
    DE50001774D1 (en) 1999-09-29 2003-05-22 Tecan Trading Ag Maennedorf Thermal cycler and lifting element for microtiter plate
    AU3096100A (en) * 1999-12-22 2001-07-03 Pe Holding B.V. A lid mechanism
    US7169355B1 (en) * 2000-02-02 2007-01-30 Applera Corporation Apparatus and method for ejecting sample well trays
    US6599484B1 (en) * 2000-05-12 2003-07-29 Cti, Inc. Apparatus for processing radionuclides
    US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
    JP4773035B2 (en) * 2000-06-28 2011-09-14 スリーエム イノベイティブ プロパティズ カンパニー Enhanced sample processing apparatus, system and method
    US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
    US6825041B2 (en) * 2001-03-16 2004-11-30 Beckman Coulter, Inc. Method and system for automated immunochemistry analysis
    DE10115848A1 (en) * 2001-03-30 2002-10-10 Biometra Biomedizinische Analy Device for thermally influencing, preferably liquid, sample material contained in a container
    US6698213B2 (en) * 2001-05-22 2004-03-02 Integrated Biosystems, Inc. Systems and methods for freezing and storing biopharmaceutical material
    GB0121827D0 (en) * 2001-09-10 2001-10-31 Bjs Company Ltd Zone heating of specimen carriers
    AU2002341644B2 (en) 2001-09-11 2008-02-28 Iquum, Inc. Sample vessels
    US20030072685A1 (en) * 2001-10-11 2003-04-17 Goldman Jeffrey A. Heat conducting sample block
    JP2005507078A (en) * 2001-10-26 2005-03-10 セクエノム， インコーポレイテッド Method and apparatus for parallel distribution of a defined volume of solid particles
    DE20117661U1 (en) * 2001-10-29 2003-03-13 MWG-BIOTECH AG, 85560 Ebersberg Apparatus for heating reaction vessel wells in micro-titration plate has base body to hold them, containing temperature control block which is moved up and down through movements of swing lid
    WO2003055973A1 (en) * 2001-12-26 2003-07-10 Olympus Corporation Reaction container and reaction container holding mechanism
    US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
    US6677151B2 (en) * 2002-01-30 2004-01-13 Applera Corporation Device and method for thermal cycling
    US20040241723A1 (en) * 2002-03-18 2004-12-02 Marquess Foley Leigh Shaw Systems and methods for improving protein and milk production of dairy herds
    DE10243209A1 (en) * 2002-03-22 2003-10-02 Endress & Hauser Wetzer Gmbh Device for cooling sampling unit comprises container for receiving sample bottle and/or sample container arranged in sampling unit, cooling unit and vaporizer
    KR100698039B1 (en) * 2002-06-14 2007-03-23 엘지.필립스 엘시디 주식회사 Cleaning jig
    US7452712B2 (en) * 2002-07-30 2008-11-18 Applied Biosystems Inc. Sample block apparatus and method of maintaining a microcard on a sample block
    US6730883B2 (en) * 2002-10-02 2004-05-04 Stratagene Flexible heating cover assembly for thermal cycling of samples of biological material
    US6905076B2 (en) * 2002-11-15 2005-06-14 Advanced Research And Technology Institute, Inc. High temperature incubation system and method for small volumes
    SE0203781D0 (en) * 2002-12-19 2002-12-19 Alphahelix Ab Holder and method for cooling or heating samples
    US20060094108A1 (en) * 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
    EP1608952B1 (en) 2002-12-20 2016-08-10 Life Technologies Corporation Assay apparatus and method using microfluidic arrays
    ES2566509T3 (en) 2003-02-05 2016-04-13 Iquum, Inc. Sample Processing
    EP1606419A1 (en) 2003-03-18 2005-12-21 Quantum Genetics Ireland Limited Systems and methods for improving protein and milk production of dairy herds
    DE10312197A1 (en) * 2003-03-19 2004-09-30 Roche Diagnostics Gmbh Sample treatment device, in particular automatic analysis device
    GB2402090B (en) * 2003-05-30 2006-12-06 Electrothermal Eng Ltd Multi-station reaction apparatus
    GB0322443D0 (en) * 2003-09-25 2003-10-29 Rts Thurnall Plc Compound storage vessel handling apparatus
    US20050202484A1 (en) 2004-02-19 2005-09-15 The Governors Of The University Of Alberta Leptin promoter polymorphisms and uses thereof
    US20050244933A1 (en) * 2004-04-28 2005-11-03 International Business Machines Corporation Method and apparatus for precise temperature cycling in chemical/biochemical processes
    US20080118955A1 (en) * 2004-04-28 2008-05-22 International Business Machines Corporation Method for precise temperature cycling in chemical / biochemical processes
    DE102004024350A1 (en) * 2004-05-17 2005-12-15 H+P Labortechnik Ag Reaction vessel and its preparation and use
    DE102004025538A1 (en) * 2004-05-25 2005-12-22 Advalytix Ag Temperature control method and apparatus for the temperature treatment of small quantities of liquid
    US20050282266A1 (en) * 2004-06-21 2005-12-22 Chun-Nan Teng Polymerase chain reactor
    US20050282270A1 (en) * 2004-06-21 2005-12-22 Applera Corporation System for thermally cycling biological samples with heated lid and pneumatic actuator
    WO2006002403A1 (en) * 2004-06-23 2006-01-05 Applera Corporation Thermal cycler
    US7799283B2 (en) * 2004-11-12 2010-09-21 Ortho-Clinical Diagnostics, Inc. Heating and cooling multiple containers or multi-chamber containers
    US8008066B2 (en) 2005-03-10 2011-08-30 Gen-Probe Incorporated System for performing multi-formatted assays
    USD564667S1 (en) 2005-07-05 2008-03-18 3M Innovative Properties Company Rotatable sample processing disk
    US7763210B2 (en) 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
    US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
    US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
    JP4187259B2 (en) * 2005-10-04 2008-11-26 キヤノン株式会社 Pressure support mechanism of structure
    JP4630786B2 (en) * 2005-10-04 2011-02-09 キヤノン株式会社 Biochemical treatment apparatus, DNA amplification and purification apparatus, and DNA testing apparatus including the apparatus
    US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
    US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
    DE102006001882A1 (en) * 2006-01-13 2007-07-19 Roche Diagnostics Gmbh Composite group of reagent carriers
    US10697987B2 (en) 2006-01-23 2020-06-30 Brooks Automation, Inc. Automated system for storing, retrieving and managing samples
    EP2051810B1 (en) 2006-01-23 2012-11-07 Nexus Biosystems, Inc., Automated system for storing, retreiving and managing samples
    US8232091B2 (en) 2006-05-17 2012-07-31 California Institute Of Technology Thermal cycling system
    JP4307478B2 (en) * 2006-12-05 2009-08-05 キヤノン株式会社 Cartridge for biochemical reaction
    ES2753136T3 (en) 2006-12-22 2020-04-07 Diasorin S P A Thermal transfer methods for microfluidic systems
    US8221300B2 (en) * 2007-07-03 2012-07-17 Yury Sherman Holder for supporting test tubes side by side on a rack, and having a resilient mounting flange connecting the tubes to allow the holder to bend and fit into an angular slot of a centrifuge rotor
    US20090055243A1 (en) 2007-08-21 2009-02-26 Jayson Lee Lusk Systems and methods for predicting a livestock marketing method
    CN101801514A (en) * 2007-08-28 2010-08-11 考贝特研究控股公司 Thermal cycler with selectively openable sample ports
    US20090098593A1 (en) * 2007-10-15 2009-04-16 Biocision, Inc. Laboratory plate thermal vault
    US8460621B2 (en) * 2007-10-15 2013-06-11 Biocision, Llc Temperature transfer stand
    US20090165574A1 (en) * 2007-12-27 2009-07-02 Finnzymes Instruments Oy Instrument and method for nucleic acid amplification
    CA2716337C (en) * 2008-02-20 2017-11-14 Streck, Inc. Thermocycler and sample vessel for rapid amplification of dna
    US20100055733A1 (en) * 2008-09-04 2010-03-04 Lutolf Matthias P Manufacture and uses of reactive microcontact printing of biomolecules on soft hydrogels
    US20100119454A1 (en) * 2008-11-03 2010-05-13 Ping Shen Use of the conserved Drosophila NPFR1 system for uncovering interacting genes and pathways important in nociception and stress response
    EP2198968B1 (en) * 2008-12-16 2018-10-17 F. Hoffmann-La Roche AG Systems and methods for monitoring a thermoelectric heating and cooling device
    CN202830041U (en) * 2009-04-03 2013-03-27 Illumina公司 Device for heating biological sample
    WO2010132800A1 (en) * 2009-05-14 2010-11-18 Streck, Inc. Specimen container, system, and method
    GB2471856A (en) * 2009-07-14 2011-01-19 Mantis Deposition Ltd Sample holder
    CN102483642B (en) * 2009-09-01 2014-12-24 生命科技公司 Thermal block assemblies and instruments that provide low thermal non-uniformity for rapid thermal cycling
    CN201837588U (en) * 2009-09-09 2011-05-18 海利克斯公司 Optical system for multiple reactions
    JP5280984B2 (en) * 2009-10-23 2013-09-04 株式会社日立ハイテクノロジーズ Thermal insulation device and analyzer equipped with the same
    US20110117607A1 (en) * 2009-11-13 2011-05-19 3M Innovative Properties Company Annular compression systems and methods for sample processing devices
    US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
    USD667561S1 (en) 2009-11-13 2012-09-18 3M Innovative Properties Company Sample processing disk cover
    USD638951S1 (en) 2009-11-13 2011-05-31 3M Innovative Properties Company Sample processing disk cover
    USD638550S1 (en) 2009-11-13 2011-05-24 3M Innovative Properties Company Sample processing disk cover
    CN103221529B (en) 2010-02-23 2015-05-27 卢米尼克斯公司 Devices and methods for integrated sample preparation, reaction and detection
    CN103675303B (en) 2010-07-23 2016-02-03 贝克曼考尔特公司 Sensing system
    US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
    GB201018624D0 (en) 2010-11-04 2010-12-22 Epistem Ltd Reaction vessel
    IT1403791B1 (en) * 2010-12-30 2013-10-31 St Microelectronics Srl METHOD FOR CALIBRATING A TEMPERATURE SENSOR OF A CHEMICAL MICROREACTOR AND ANALYZER FOR BIOCHEMICAL ANALYSIS
    AU2012222178B2 (en) 2011-02-24 2014-12-18 Gen-Probe Incorporated Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector
    TW201239088A (en) * 2011-03-22 2012-10-01 Genereach Biotechnology Corp Convective polymerase chain reaction device
    TW201239089A (en) * 2011-03-22 2012-10-01 Genereach Biotechnology Corp Convective polymerase chain reaction device
    TWI563080B (en) * 2011-03-24 2016-12-21 Genereach Biotechnology Corp Method for controlling thermosiphon circulation in biochemical reaction
    CA2834790C (en) 2011-05-04 2019-04-09 Luminex Corporation Apparatus and methods for integrated sample preparation, reaction and detection
    KR102110174B1 (en) 2011-05-18 2020-05-14 디아소린 에스.피.에이. Systems and methods for volumetric metering on a sample processing device
    US9067205B2 (en) 2011-05-18 2015-06-30 3M Innovative Properties Company Systems and methods for valving on a sample processing device
    USD672467S1 (en) 2011-05-18 2012-12-11 3M Innovative Properties Company Rotatable sample processing disk
    CN105170203B (en) 2011-05-18 2017-07-18 3M创新有限公司 Material system and method present in sample processing apparatus of the selected volume of detection
    WO2012166913A1 (en) 2011-06-01 2012-12-06 Streck, Inc. Rapid thermocycler system for rapid amplification of nucleic acids and related methods
    WO2013040491A2 (en) 2011-09-15 2013-03-21 Shafer David A Probe: antiprobe compositions for high specificity dna or rna detection
    US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
    US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
    JP6062449B2 (en) 2011-11-07 2017-01-18 ベックマン コールター， インコーポレイテッド Specimen container detection
    CN104040352B (en) 2011-11-07 2016-09-07 贝克曼考尔特公司 Mechanical arm
    EP2776843B1 (en) 2011-11-07 2019-03-27 Beckman Coulter, Inc. Centrifuge system and workflow
    US9506943B2 (en) 2011-11-07 2016-11-29 Beckman Coulter, Inc. Aliquotter system and workflow
    KR20140092378A (en) 2011-11-07 2014-07-23 베크만 컬터, 인코포레이티드 System and method for processing samples
    WO2013070748A1 (en) 2011-11-07 2013-05-16 Beckman Coulter, Inc. Magnetic damping for specimen transport system
    EP2784150B1 (en) * 2011-11-22 2019-05-15 GeneReach Biotechnology Corp. Device for polymerase chain reaction via thermal convection
    JP5772984B2 (en) * 2011-12-22 2015-09-02 株式会社島津製作所 Container installation mechanism and autosampler
    CN103987835A (en) * 2012-03-09 2014-08-13 瑞基海洋生物科技股份有限公司 Heat convection speed control device and method for biochemical reaction
    EP2883039A1 (en) 2012-08-10 2015-06-17 Streck Inc. Real-time optical system for polymerase chain reaction
    WO2014113785A1 (en) * 2013-01-18 2014-07-24 Biomeme Incorporated Analytic device
    SG11201506351TA (en) * 2013-02-18 2015-09-29 Theranos Inc Systems and methods for multi-analysis
    US10001497B2 (en) 2013-03-15 2018-06-19 Abbott Laboratories Diagnostic analyzers with pretreatment carousels and related methods
    EP4571318A3 (en) 2013-03-15 2025-09-17 Abbott Laboratories Automated diagnostic analyzers having vertically arranged carousels and related methods
    US9335338B2 (en) 2013-03-15 2016-05-10 Toshiba Medical Systems Corporation Automated diagnostic analyzers having rear accessible track systems and related methods
    WO2014149268A1 (en) 2013-03-19 2014-09-25 Life Technologies Corporation Thermal cycler cover
    US10006861B2 (en) 2013-06-28 2018-06-26 Streck, Inc. Devices for real-time polymerase chain reaction
    EP3017035A4 (en) * 2013-07-01 2018-09-05 Hakalehto, Eino Elias Device for microbial cultivation with options for field sterilization and gas generation
    SE538287C2 (en) * 2013-09-30 2016-04-26 Symcel Sverige AB Sample holder adapted to isothermal calorimetry of parallel samples
    SE537326C2 (en) * 2013-09-30 2015-04-07 Symcel Sverige AB Provvial
    GB201319759D0 (en) * 2013-11-08 2013-12-25 Thomsen Lars Device and method for heating a fluid chamber
    JP6087264B2 (en) 2013-11-29 2017-03-01 株式会社日立ハイテクノロジーズ Component analyzer, medicinal effect analyzer, and analysis method
    WO2015126621A1 (en) * 2014-02-18 2015-08-27 Life Technologies Corporation Apparatuses, systems and methods for providing scalable thermal cyclers and isolating thermoelectric devices
    GB2526520B (en) * 2014-04-04 2021-08-18 It Is Int Ltd Biochemical reaction system
    GB2592541B (en) * 2014-04-04 2021-12-15 It Is International Ltd Biochemical reaction system
    CN104130933B (en) * 2014-08-02 2016-01-13 张金木 A kind of fluorescence constant temperature PCR amplification instrument
    EP3056277B1 (en) * 2015-02-12 2019-09-04 QIAGEN Lake Constance GmbH Tempering clamp
    JP6758026B2 (en) * 2015-04-17 2020-09-23 株式会社日立ハイテク Component analyzer, drug component analyzer, component analysis method and drug component analysis method
    DE102015121362B4 (en) * 2015-12-08 2018-05-24 Analytik Jena Ag Temperature control device with a reaction vessel
    SG11201805140VA (en) * 2015-12-22 2018-07-30 Life Technologies Corp Systems and methods for a thermal cycler heated cover
    US10029260B2 (en) 2016-05-24 2018-07-24 Taj King Centrifuge tube holding assembly
    WO2018038943A1 (en) * 2016-08-11 2018-03-01 Siemens Healthcare Diagnostics Inc. Heating device for a filtration assembly
    US10427162B2 (en) 2016-12-21 2019-10-01 Quandx Inc. Systems and methods for molecular diagnostics
    US10328147B2 (en) 2017-03-24 2019-06-25 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Herpes simplex virus type-1(HSV-1) vaccine strain VC2 generating an anti-EHV-1 immune response
    DE102017005835B4 (en) * 2017-06-20 2020-04-02 Diehl Metering Gmbh Device for the mobile determination of a property of a liquid, solid or gaseous sample
    CN107539355A (en) * 2017-09-13 2018-01-05 重庆铜山天询科技有限公司 It is easy to carry the transportation equipment of experiment equipment
    EP3682024A4 (en) 2017-09-15 2021-05-12 Biomeme, Inc. METHODS AND SYSTEMS FOR AUTOMATIC SAMPLE PROCESSING
    CN111712581A (en) 2017-12-15 2020-09-25 生米公司 Portable device and method for analyzing samples
    JP2020085748A (en) * 2018-11-29 2020-06-04 立山マシン株式会社 Sample temperature controller
    JP7483745B2 (en) 2019-03-21 2024-05-15 バイオミーム インコーポレイテッド Multifunctional analytical device
    US20220401960A1 (en) * 2019-08-08 2022-12-22 The Regents Of The University Of California High throughput radiochemistry system
    DE102019124588A1 (en) * 2019-09-12 2021-03-18 Biometra GmbH Temperature control device
    CN110721757A (en) * 2019-10-22 2020-01-24 威尚生物技术（合肥）有限公司 Reagent tube storage device for biological agent
    WO2021257348A1 (en) * 2020-06-15 2021-12-23 Bio-Rad Laboratories, Inc. Pcr sample block temperature uniformity
    CN215886967U (en) * 2020-06-30 2022-02-22 利多(香港)有限公司 Detection analyzer
    US20230271189A1 (en) * 2020-07-31 2023-08-31 Seegene, Inc. Thermal cycler comprising damping module
    JP2023545631A (en) 2020-09-18 2023-10-31 バイオミーム，インコーポレイテッド Transportable devices and methods for analyzing samples
    CN114437919A (en) * 2020-10-30 2022-05-06 中元汇吉生物技术股份有限公司 Hot lid device and nucleic acid detection analyzer
    JP7327360B2 (en) * 2020-11-19 2023-08-16 横河電機株式会社 Heat treatment system, nucleic acid extraction system, nucleic acid analysis system
    EP4176971A1 (en) * 2021-11-03 2023-05-10 Wizbiosolutions Inc. Integrated molecular diagnosis apparatus
    TWI859922B (en) * 2022-08-19 2024-10-21 緯創資通股份有限公司 Temperature control assembly

    Citations (4)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    DE3024210A1 (en) * 1980-06-27 1982-01-14 Norddeutsche Seekabelwerke Ag, 2890 Nordenham Metal block thermostat - has reaction vessels automatically inserted and withdrawn by spring mechanism after standard period
    DE8804938U1 (en) * 1988-04-14 1988-05-26 Deutsche Metrohm GmbH & Co, 7024 Filderstadt Device for the chemical analysis and/or treatment of substances contained in several sample vessels
    EP0488769A2 (en) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
    WO1992020778A1 (en) * 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control

    Family Cites Families (31)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    JPS55136957A (en) * 1979-04-14 1980-10-25 Olympus Optical Co Ltd Automatic analyzer
    SE428609B (en) * 1981-03-20 1983-07-11 Coulter Electronics SAMPLES FOR MIXING AND SAMPLING BLOOD OR SIMILAR SEDIMENTAL LIQUID
    JPS57158551A (en) * 1981-03-27 1982-09-30 Hitachi Ltd Automatic liquid sampler
    JPS5862566A (en) * 1981-10-09 1983-04-14 Sekonitsuku:Kk Liquid vessel with temperature control
    JPS5862565A (en) * 1981-10-09 1983-04-14 Sekonitsuku:Kk Control of liquid temperature
    JPH0234048B2 (en) * 1981-10-09 1990-08-01 Sekonitsuku Kk EKITAIYOKINOONDOSEIGYOSOCHI
    JPS58189559A (en) * 1982-04-30 1983-11-05 Toshiba Corp Cap used for sample cup
    US4539855A (en) * 1984-05-03 1985-09-10 Eastman Kodak Company Apparatus for transferring liquid out of a capped container, and analyzer utilizing same
    JPS60241884A (en) * 1984-05-15 1985-11-30 Tokyo Daigaku Automatic cycling reaction device and automatic analysis device using the same
    JPS61212764A (en) * 1985-03-18 1986-09-20 Toshiba Corp Thermostatic cell for automatic chemical analyzer
    CA1339653C (en) * 1986-02-25 1998-02-03 Larry J. Johnson Appartus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
    JPS62235570A (en) * 1986-04-05 1987-10-15 Japan Spectroscopic Co Specimen sampling apparatus
    US4933146A (en) * 1986-07-11 1990-06-12 Beckman Instruments, Inc. Temperature control apparatus for automated clinical analyzer
    DE3769788D1 (en) * 1987-02-25 1991-06-06 Hewlett Packard Gmbh APPARATUS FOR PERFORMING CHEMICAL REACTIONS.
    JPS63137865U (en) * 1987-02-28 1988-09-12
    JPS63137866U (en) * 1987-02-28 1988-09-12
    JPH0350451Y2 (en) * 1987-07-16 1991-10-28
    JPH06103315B2 (en) * 1987-08-14 1994-12-14 株式会社東芝 Dispensing nozzle device of automatic chemical analyzer
    US4951512A (en) * 1988-06-23 1990-08-28 Baxter International Inc. System for providing access to sealed containers
    US4865986A (en) * 1988-10-06 1989-09-12 Coy Corporation Temperature control apparatus
    DE8813773U1 (en) * 1988-11-03 1989-01-05 Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV, 37073 Göttingen Device for optionally setting the temperature of a sample to different values
    US5123477A (en) * 1989-05-02 1992-06-23 Unisys Corporation Thermal reactor for biotechnological processes
    GB8917963D0 (en) * 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
    US5171531A (en) * 1989-10-25 1992-12-15 Hewlett-Packard Company Apparatus for automatic delivery of a vial to an automated sampling system
    JPH041449U (en) * 1990-04-19 1992-01-08
    US5207987A (en) * 1990-05-21 1993-05-04 Pb Diagnostic Systems Inc. Temperature controlled chamber for diagnostic analyzer
    AT397610B (en) * 1990-06-01 1994-05-25 Avl Verbrennungskraft Messtech DEVICE FOR TAKING BODY LIQUIDS
    US5455175A (en) * 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
    US5187084A (en) * 1990-06-22 1993-02-16 The Dow Chemical Company Automatic air temperature cycler and method of use in polymerose chain reaction
    CA2130013C (en) * 1993-09-10 1999-03-30 Rolf Moser Apparatus for automatic performance of temperature cycles
    JP3034954B2 (en) * 1993-10-22 2000-04-17 アボツト・ラボラトリーズ Reaction tubes and methods of use to minimize contamination

    Patent Citations (4)

    * Cited by examiner, † Cited by third party
    Publication number Priority date Publication date Assignee Title
    DE3024210A1 (en) * 1980-06-27 1982-01-14 Norddeutsche Seekabelwerke Ag, 2890 Nordenham Metal block thermostat - has reaction vessels automatically inserted and withdrawn by spring mechanism after standard period
    DE8804938U1 (en) * 1988-04-14 1988-05-26 Deutsche Metrohm GmbH & Co, 7024 Filderstadt Device for the chemical analysis and/or treatment of substances contained in several sample vessels
    EP0488769A2 (en) * 1990-11-29 1992-06-03 The Perkin-Elmer Corporation Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control
    WO1992020778A1 (en) * 1991-05-24 1992-11-26 Kindconi Pty Limited Biochemical reaction control

    Also Published As

    Publication number Publication date
    ATE199222T1 (en) 2001-03-15
    US5616301A (en) 1997-04-01
    EP0807468A2 (en) 1997-11-19
    DE59410021D1 (en) 2002-01-31
    DE59409658D1 (en) 2001-03-29
    EP0642831A1 (en) 1995-03-15
    EP0807467B1 (en) 2001-12-19
    DE59410020D1 (en) 2002-01-31
    GR3035810T3 (en) 2001-07-31
    DK0642831T3 (en) 2001-05-21
    CA2130013A1 (en) 1995-03-11
    ATE211023T1 (en) 2002-01-15
    EP0807468A3 (en) 1998-06-10
    PT642831E (en) 2001-07-31
    EP0807468B1 (en) 2001-12-19
    ES2169295T3 (en) 2002-07-01
    ES2169294T3 (en) 2002-07-01
    CA2130013C (en) 1999-03-30
    ATE211024T1 (en) 2002-01-15
    EP0642831B1 (en) 2001-02-21
    JPH07151764A (en) 1995-06-16
    ES2155456T3 (en) 2001-05-16
    US5795547A (en) 1998-08-18
    EP0807467A2 (en) 1997-11-19

    Similar Documents

    Publication Publication Date Title
    EP0807467A3 (en) Device for automatically carrying out polymerase chain reactions
    EP0642828B1 (en) Array of reaction vessels for a device for automatically carrying out temperature cycling
    US5398837A (en) Cell culture flask and closure
    DE60211760T2 (en) Apparatus and method for processing a plurality of reaction mixtures in parallel
    DE69325567T2 (en) Cultural vessel
    DE68923370D1 (en) A mechanism with a handle for opening, closing and locking the lid of a pressure cooker with a large capacity.
    WO1999007471A1 (en) System for providing biological materials
    EP0281958A3 (en) Device for controlling the temperature and mixing the contents of the vessels of a micro-titration plate
    CA2116379A1 (en) Fermentation Plant
    CA2255850A1 (en) Rotary thermocycling apparatus
    EP0751809A1 (en) Process and device for preparing and/or extracting samples by means of a vaporisable agent at high temperature
    CA2370669A1 (en) Process for solid phase synthesis
    EP0989907B1 (en) Device for conducting a plurality of chemical, biochemical or physical procedures in parallel
    DE69018288D1 (en) Device for mixing and discharging granular solids from a container.
    EP0822251A3 (en) Plant for the production of biogas
    SU997778A1 (en) Apparatus for ajitating
    DE3101308A1 (en) "DEVICE FOR HEATING POWDER-SHAPED PRODUCTS"
    DE7506028U (en) Device for mixing and foaming liquid and semi-liquid materials in the designated lid container
    DE68911105T2 (en) Heater for dental impression materials.
    JPH053034Y2 (en)
    JPS58208665A (en) Chemical manipulator
    DE9318962U1 (en) Three-spiral mixing basket with barbed tips
    DE7709727U1 (en) DEVICE FOR SEPARATING SOLIDS FROM PASTOES OR MEASURES SUSPENDED IN A LIQUID MEDIUM
    WO1994023279B1 (en) Device for determining the binder content of a bituminous construction material sample
    DE2305169A1 (en) Laboratory dyeing vessel - with a ptfe coated magnetic stirrer in a base compartment

    Legal Events

    Date Code Title Description
    PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

    Free format text: ORIGINAL CODE: 0009012

    17P Request for examination filed

    Effective date: 19970724

    AC Divisional application: reference to earlier application

    Ref document number: 642831

    Country of ref document: EP

    AK Designated contracting states

    Kind code of ref document: A2

    Designated state(s): AT BE CH DE DK ES FR GB IT LI NL

    PUAL Search report despatched

    Free format text: ORIGINAL CODE: 0009013

    AK Designated contracting states

    Kind code of ref document: A3

    Designated state(s): AT BE CH DE DK ES FR GB IT LI NL

    17Q First examination report despatched

    Effective date: 19991112

    GRAG Despatch of communication of intention to grant

    Free format text: ORIGINAL CODE: EPIDOS AGRA

    GRAG Despatch of communication of intention to grant

    Free format text: ORIGINAL CODE: EPIDOS AGRA

    GRAG Despatch of communication of intention to grant

    Free format text: ORIGINAL CODE: EPIDOS AGRA

    GRAH Despatch of communication of intention to grant a patent

    Free format text: ORIGINAL CODE: EPIDOS IGRA

    GRAH Despatch of communication of intention to grant a patent

    Free format text: ORIGINAL CODE: EPIDOS IGRA

    GRAA (expected) grant

    Free format text: ORIGINAL CODE: 0009210

    AC Divisional application: reference to earlier application

    Ref document number: 642831

    Country of ref document: EP

    AK Designated contracting states

    Kind code of ref document: B1

    Designated state(s): AT BE CH DE DK ES FR GB IT LI NL

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: NL

    Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

    Effective date: 20011219

    REF Corresponds to:

    Ref document number: 211023

    Country of ref document: AT

    Date of ref document: 20020115

    Kind code of ref document: T

    REG Reference to a national code

    Ref country code: CH

    Ref legal event code: NV

    Representative=s name: AMMANN PATENTANWAELTE AG BERN

    Ref country code: CH

    Ref legal event code: EP

    REG Reference to a national code

    Ref country code: GB

    Ref legal event code: IF02

    GBT Gb: translation of ep patent filed (gb section 77(6)(a)/1977)

    Effective date: 20011231

    REF Corresponds to:

    Ref document number: 59410020

    Country of ref document: DE

    Date of ref document: 20020131

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: DK

    Free format text: LAPSE BECAUSE OF FAILURE TO SUBMIT A TRANSLATION OF THE DESCRIPTION OR TO PAY THE FEE WITHIN THE PRESCRIBED TIME-LIMIT

    Effective date: 20020319

    REG Reference to a national code

    Ref country code: CH

    Ref legal event code: NV

    Representative=s name: VENTOCILLA PATENT AG

    NLV1 Nl: lapsed or annulled due to failure to fulfill the requirements of art. 29p and 29m of the patents act
    REG Reference to a national code

    Ref country code: ES

    Ref legal event code: FG2A

    Ref document number: 2169294

    Country of ref document: ES

    Kind code of ref document: T3

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: BE

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20020831

    Ref country code: AT

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20020831

    PLBE No opposition filed within time limit

    Free format text: ORIGINAL CODE: 0009261

    STAA Information on the status of an ep patent application or granted ep patent

    Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

    26N No opposition filed
    BERE Be: lapsed

    Owner name: F. *HOFFMANN-LA ROCHE A.G.

    Effective date: 20020831

    NLV1 Nl: lapsed or annulled due to failure to fulfill the requirements of art. 29p and 29m of the patents act
    REG Reference to a national code

    Ref country code: CH

    Ref legal event code: NV

    Representative=s name: ROCHE DIAGNOSTICS AG

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: ES

    Payment date: 20100805

    Year of fee payment: 17

    Ref country code: CH

    Payment date: 20100726

    Year of fee payment: 17

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: IT

    Payment date: 20100818

    Year of fee payment: 17

    Ref country code: FR

    Payment date: 20100819

    Year of fee payment: 17

    Ref country code: DE

    Payment date: 20100831

    Year of fee payment: 17

    PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

    Ref country code: GB

    Payment date: 20100708

    Year of fee payment: 17

    REG Reference to a national code

    Ref country code: CH

    Ref legal event code: PL

    GBPC Gb: european patent ceased through non-payment of renewal fee

    Effective date: 20110831

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: CH

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20110831

    Ref country code: LI

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20110831

    REG Reference to a national code

    Ref country code: FR

    Ref legal event code: ST

    Effective date: 20120430

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: IT

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20110831

    REG Reference to a national code

    Ref country code: DE

    Ref legal event code: R119

    Ref document number: 59410020

    Country of ref document: DE

    Effective date: 20120301

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: FR

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20110831

    Ref country code: GB

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20110831

    REG Reference to a national code

    Ref country code: ES

    Ref legal event code: FD2A

    Effective date: 20130604

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: DE

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20120301

    PG25 Lapsed in a contracting state [announced via postgrant information from national office to epo]

    Ref country code: ES

    Free format text: LAPSE BECAUSE OF NON-PAYMENT OF DUE FEES

    Effective date: 20110901