JPS58189559A - Cap used for sample cup - Google Patents

Abstract

PURPOSE:To reduce an measurement error caused by evaporation of a sample without impairing operability of sampling, by forming a small hole for inserting a sampling nozzle through the cap. CONSTITUTION:A small hole 4a is formed through the center of the cap 4 used for the sampling cup to enable insertion of the sapling nozzle. Such a constitution suppresses evaporation of the sample contained in the sampling cup without impairing operation of sampling, and reduces a measurement error due to its evaporation.

Description

[Detailed Description of the Invention] [Technical Field of the Invention] The present invention belongs to the field of clinical testing that measures the body fluid components of a subject, and relates to a container (hereinafter also referred to as a sample cup) for storing a sample. This invention relates to a cap for a sample cup.

[Technical background of the invention and its problems] A book shown in FIG. 1 has been cited as a sample cup used in clinical tests. FIG. 1 is a schematic perspective view showing an example of a lotus/pull cup and a cap for a sample cup (hereinafter also referred to as a cap). In Figure 1,
The sample cup 1 has a stiff cylindrical shape with one end open, and the sample 2 is stored inside the cup. 2α indicates the liquid level of specimen 2. The sir//luke cap 3 is attached to the open end of the sample cup 1 with an appropriate fit.

For example, the sample 2 may be a serum component obtained by centrifuging blood, but when the sample 2 is stored in the sample cup 1, the sample cup cap 3 is quickly attached to the sample 2. If it is not sealed, the concentration of the sample 2 will be concentrated due to evaporation, and the accuracy of analytical measurements will be lost. However, at least during analytical measurements, it is necessary to remove the cap 3 in order to sample the specimen 2. At this time, the evaporation rate of the sample 2 is determined by the open area S of the sample cup 1 shown in FIG. 1, and the height from the liquid level 2α to the open end! Although it depends on other factors such as ambient temperature, humidity, liquid of sample 2, air circulation, etc., if the setup time and measurement time for Kiro1 analysis measurement lasts several hours, this Evaporation lids cannot be ignored. Therefore, except when it is necessary to remove the cap for sampling, cap 2 is attached to sample cup 1.
However, it is desirable to prevent evaporation of specimen 2 by attaching a

2 caps at any time for each of the hundreds of sample cups (10)
It is a great burden for workers to put on caps, and in cases other than long-term storage, they often do not have to put on caps.

[Object of the invention] The present invention has been made in view of the above circumstances.

It is an object of the present invention to provide a cap for a sample cup that can reduce measurement errors caused by evaporation of a specimen without impairing the operability of sampling.

[Summary of the invention]

To achieve the above object, the present invention provides a cap for a sample cup that is removably attached to the open end of a sample cup that stores a sample to be aspirated in a predetermined amount by a sampling nozzle for analysis and measurement. This device is characterized by providing a small hole with a size that allows the insertion and removal of the sampling nozzle, thereby suppressing the evaporation of the sample.

[Embodiments of the invention]

Hereinafter, one embodiment of the present invention will be described with reference to the drawings. FIG. 2 (αl, fA+ is a front view and a sectional view showing a cap for a sample cup, which is an embodiment of the present invention.
The cap 4 shown in FIG. 2 is different from the conventional cap 6 shown in FIG. 1 in that a small hole 4α is provided in the center of the cap 4, for example. Although the structure of the present invention is extremely simple, the provision of this small hole 4α can produce a great effect.

Hereinafter, the experimental data shown in FIGS. 6, 4, 5, and 6 will be added to this reference to explain the effects.

FIG. 6 shows a device containing serum (tom) as a specimen 2 in the sandal cup 1, and a cap 4 with a hole diameter of HL (small hole 4α of IU, 2111.30.41EI) attached. It is a characteristic diagram showing the serum amount of each book without the cap 4 after being left for one hour as a percentage of the serum amount at the time of storage.

Measurement conditions: room temperature 20.5±0.5℃ 9gk degree 48~
The test was carried out under 64 cycles, and the amount of serum after standing was measured using a gravimetric method using a chemical balance. The inner diameter of the sample cup 1 for storing serum is 12.5 m. As shown in FIG. 5, the amount of serum evaporation after being left for 2 hours is 234% in the case of the cap, while the amount of serum evaporation in the case of the cap 4 with the small hole 4a of 11Il in diameter is 234%. , pore diameter 2
The percentage is 0.24% for U, 045% for pore size 5, and 0.61% for 4 mjI.

When this is converted into an evaporation rate ratio, it is compared to the case without a cap. The evaporation rate ratio when a cap 4 with a small hole 4α with a pore diameter of 1 day is attached is α056%/264%Φ1
/40, which can produce an extremely large effect. In addition, a small hole 4 with a hole diameter of 4U is used for the case without a cap.
The evaporation rate ratio when the cap 4 having a is attached is:
061%/234%÷1/4, and in this case, this sufficient effect can be achieved. Next, referring to Figure 4, we will explain the protein concentration in serum due to serum evaporation and the standing time T.
Explain the relationship between Protein concentration is total protein f1/serum t
It is represented by (d stone). As shown in Figure 4, the small hole 4α
When cap 4 with
In the time range, it is within the error range of practical measurement, and the influence of evaporation can be ignored. FIG. 5 is a characteristic diagram showing the relationship between the concentration of lactate dehydrogenase in serum due to serum evaporation and the standing time T. Lactate dehydrogenase concentration is 1 t of lactate dehydrogenase activity (IIJ)/1 t of serum.
6. If a cap 4 having a VC1 small hole 4α is attached as shown in FIG.

Even if you leave it for 8 hours, there will be no significant difference in practical use.

Next, with reference to FIG. 6, the relationship between the rate of decrease in serum volume due to serum evaporation (positive) and the diameter of the small hole 4a in the cap 4 will be displayed and explained for each standing time. The rate of decrease in serum volume is based on the serum volume when stored in sample cup 1, and the serum volume after being left for a predetermined time is measured by the gravimetric method using a chemical balance, and the amount of evaporation is expressed as a percentage of the serum volume when stored. This is what I did.

In terms of the hole diameter taken on the horizontal axis, "No cap 1" refers to the opening diameter of the sample cup 1 in this experiment, which corresponds to 125U.As shown in Fig. 6, the hole diameter of the small hole 4α is 1U.
It goes without saying that in the case of ~4u, the effect of suppressing evaporation is extremely large as mentioned above, but when the pore size is 6ws
It can be seen that even in this case, the amount of evaporation can be suppressed to 172 or less compared to the case without a cap and the pore diameter is 1250.

As explained above, the provision of the small hole 4α in the cap 4 has a great effect on suppressing the amount of serum evaporation. In order to reduce the burden of attaching and detaching the cap 4, the cap 4 must be attached to the K during one analysis and measurement, that is, the time during which the sample 2 stored in the sample cup 1 is aspirated and subjected to measurement. The present invention naturally satisfies the above conditions, and this will be explained with reference to FIG. 7 using an automatic chemical analyzer as an example. In FIG. 7, a cap 4 having a small hole 4a is attached to the open end of a sample cup 1 containing a specimen 2 (not shown). A sampling nozzle 5 for aspirating the specimen 2 in the sample cup 1 is connected to the sample cup 1.
is arranged above the sliding member 6 with the arrow A shown in the figure.
It is attached with a K47 that can be raised and lowered depending on the direction. nl
The movable member 6 has a groove 6α that is guided by the rail 7 which is installed in the direction of the arrow B'75 in the figure, and a wire 9 that is stretched between the drive pulley 8 and the driven pulley (not shown). Fixed in some parts.

Specimen 2 in the automated chemical analyzer arranged as above.
To sample, as shown in FIG. 7, the sampling nozzle 5 set above the small hole 4α in the cap 4 is lowered, and the lower end of the sampling nozzle 5 is inserted into the sample cup 1 through the small hole 4α. And h? not shown? After suctioning the specimen 2 by the pump, the sunblink nozzle 5 is raised. Thereafter, by rotating the driving pulley 8 to move the sliding member 6 along the rail 7 and discharging the specimen 2 into a reaction tube (not shown), the specimen 2 can be subjected to analysis and measurement.

As explained above, the small hole 4α in the cap 4 is used for inserting and removing the sampling nozzle 5. Therefore, the hole diameter of the small hole 4a is limited by the outer diameter of the bling nozzle 5 and the movement accuracy of the sliding member 6, but generally the outer diameter of the sampling nozzle 5 is about 0.61j, and the small hole It is sufficient that the pore diameter of 4 m is 1 m11 or more.

In addition, if the sliding member 6 has poor movement accuracy, for example, as shown in FIG.
A tapered guide member 10 whose upper end has a large opening diameter and whose lower end matches the diameter of the small hole 4g is provided, and the sampling nozzle 5 is moved to the small hole AaK 4 by sucking the movement error of the sliding member 6.
＜You can do it like this. 1. The cap 4 according to the present invention is not limited to those used for automatic chemical analysis as in the above embodiments, but it is absolutely certain that the cap 4 of the present invention can exhibit sufficient effects not only for those used for automatic chemical analysis as in the above-mentioned embodiments, but also for those used for manual analysis and measurement. . In addition, since the cap 4 is detachable from the sample cup 1, the cap 4 can be removed and removed as in the conventional case when storing the specimen 2 in the sample cup 1 or when cleaning the sample cup 1. . Similarly, the shape of the small hole 4α is limited to a round hole and a square hole.

It may also be an elliptical hole or the like.

〔Effect of the invention〕

As explained above, according to the present invention, by providing a small hole in the cap with a size that allows the sampling nozzle to be inserted and removed, measurement errors caused by evaporation of the sample can be reduced without impairing the operability of sampling. It is possible to provide a cap for a sample cup that can achieve the following.

[Brief explanation of the drawing]

FIG. 1 is a schematic perspective view showing an example of a conventional sample cup and sample cup gap, FIG. 2 is a schematic perspective view showing a sample cup camp according to an embodiment of the present invention, FIGS. 3 and 4 , Figure 5 is a characteristic diagram showing the relationship between the standing time T and the serum volume after storage, total protein concentration, and lactate dehydrogenase concentration with respect to the serum volume at storage, for each cap pore diameter.
The figure is a characteristic diagram showing the relationship between the pore diameter of the cap and the amount of evaporation with respect to the amount of serum when stored, for each standing time T. Figure 7 is a schematic perspective view illustrating how the cap of the present invention is used, and Figure 8 is 1-7 is a schematic perspective view showing the use of the cap according to the present invention, which is equipped with a guide member; FIG. 1... Sunphool cup, 2... Sample, 4...
・Cap for sample cup, 4a...small hole,
5...Sampling nozzle

Claims (1)

[Claims] A cap for a sample cup that is removably attached to the open end of a sample cup that stores a predetermined amount of sample aspirated by a sampling nozzle and subjected to analysis measurement, wherein the small hole has a size that allows the sampling nozzle to be inserted and removed. A cap for a sample cup characterized by suppressing the amount of evaporation of a sample.