EP0782614A1 - Introduction directe de substances etrangeres dans des cellules - Google Patents

Introduction directe de substances etrangeres dans des cellules

Info

Publication number
EP0782614A1
EP0782614A1 EP95935656A EP95935656A EP0782614A1 EP 0782614 A1 EP0782614 A1 EP 0782614A1 EP 95935656 A EP95935656 A EP 95935656A EP 95935656 A EP95935656 A EP 95935656A EP 0782614 A1 EP0782614 A1 EP 0782614A1
Authority
EP
European Patent Office
Prior art keywords
microprobes
cells
biological material
support
microns
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP95935656A
Other languages
German (de)
English (en)
Inventor
William Stuart Trimmer
Peter Ping Ling
Sarwar Hashmi
Randy Gaugler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rutgers State University of New Jersey
Original Assignee
Rutgers State University of New Jersey
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rutgers State University of New Jersey filed Critical Rutgers State University of New Jersey
Publication of EP0782614A1 publication Critical patent/EP0782614A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
    • A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
    • A01K67/0335—Genetically modified worms
    • A01K67/0336—Genetically modified Nematodes, e.g. Caenorhabditis elegans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
    • C07K14/4354—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from nematodes
    • C07K14/43545—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from nematodes from Caenorhabditis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Sustainable Development (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La présente invention concerne un procédé simple, peu onéreux et précis permettant d'introduire une substance étrangère dans une population prédéterminée de cellules cibles. En un premier temps, le procédé consiste à disposer (a) des microsondes biologiquement inertes positionnées sur un support, (b) une masse solide ou quasi-solide de cellules cibles définissant une interface avec les microsondes, et (c) une substance biologique à l'interface. En un second temps, le procédé consiste à faire entrer les cellules en contact avec les microsondes, de sorte que les microsondes perforent de façon non létale les parois ou membranes cellulaires des cellules. Les microsondes, qui font de préférence corps avec le support, sont préparées par attaque chimique d'une plaquette unique d'un cristal telle que le silicium. La forme des microsondes est de préférence pyramidale. Le contact entre cellules cibles et microsondes peut se faire soit in vitro, soit in situ. Ce procédé convient avantageusement à presque tous les types de cellules et de substances biologiques susceptibles d'être introduites dans les cellules décrites ici.
EP95935656A 1994-09-30 1995-09-29 Introduction directe de substances etrangeres dans des cellules Withdrawn EP0782614A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US334606 1981-12-28
US31533694A 1994-09-30 1994-09-30
US315336 1994-09-30
US33460694A 1994-11-04 1994-11-04
PCT/US1995/012381 WO1996010630A1 (fr) 1994-09-30 1995-09-29 Introduction directe de substances etrangeres dans des cellules

Publications (1)

Publication Number Publication Date
EP0782614A1 true EP0782614A1 (fr) 1997-07-09

Family

ID=26979842

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95935656A Withdrawn EP0782614A1 (fr) 1994-09-30 1995-09-29 Introduction directe de substances etrangeres dans des cellules

Country Status (4)

Country Link
EP (1) EP0782614A1 (fr)
AU (1) AU3758595A (fr)
CA (1) CA2201315A1 (fr)
WO (1) WO1996010630A1 (fr)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9815819D0 (en) * 1998-07-22 1998-09-16 Secr Defence Transferring materials into cells and a microneedle array
EP1124939B1 (fr) * 1998-10-08 2003-04-23 AstraZeneca AB Injecteur de cellules microfabrique
MXPA03003815A (es) 2000-10-26 2004-08-12 Johnson & Johnson Dispositivos de suministro transdermico de farmaco que tienen microrresaltos recubiertos.
KR20050054483A (ko) * 2002-06-28 2005-06-10 알자 코포레이션 코팅된 미세돌출부를 갖는 경피용 약물 전달 장치
AU2003251831B2 (en) * 2002-07-19 2009-06-11 3M Innovative Properties Company Microneedle devices and microneedle delivery apparatus
BRPI0412029A (pt) 2003-06-30 2006-09-05 Alza Corp formulações para microprojeções revestidas contendo contra-ìons não-voláteis
US8961477B2 (en) 2003-08-25 2015-02-24 3M Innovative Properties Company Delivery of immune response modifier compounds
US7132242B2 (en) * 2003-11-10 2006-11-07 Hewlett-Packard Development Company, L.P. Method and device for targeted delivery of materials to selected single cells
US8057842B2 (en) 2004-11-18 2011-11-15 3M Innovative Properties Company Method of contact coating a microneedle array
AU2005306426B2 (en) 2004-11-18 2011-04-28 3M Innovative Properties Company Masking method for coating a microneedle array
JP5015787B2 (ja) 2004-11-18 2012-08-29 スリーエム イノベイティブ プロパティズ カンパニー マイクロニードルアレイの接触コーティング法
WO2006055802A1 (fr) 2004-11-18 2006-05-26 3M Innovative Properties Company Attache et applicateur pour jeu de micro-aiguilles
WO2006062974A2 (fr) 2004-12-07 2006-06-15 3M Innovative Properties Company Procede de moulage d'une microaiguille
WO2006108185A1 (fr) 2005-04-07 2006-10-12 3M Innovative Properties Company Systeme et procede de captage de retroaction d’un outil
WO2007002523A2 (fr) 2005-06-24 2007-01-04 3M Innovative Properties Company Timbre repliable et son procede d'application
EP1896115B2 (fr) 2005-06-27 2020-01-22 3M Innovative Properties Company Ensemble de cartouches a micro-aiguilles
AU2006261898B2 (en) 2005-06-27 2011-11-03 3M Innovative Properties Company Microneedle array applicator device
WO2007061781A1 (fr) 2005-11-18 2007-05-31 3M Innovative Properties Company Compositions pouvant être revêtues, revêtements dérivés de celles-ci et micro-réseaux comprenant de tels revêtements
WO2007076458A1 (fr) * 2005-12-21 2007-07-05 Primegen Biotech Llc Puce a micro-injecteur
AU2006336187A1 (en) 2005-12-28 2007-07-26 Alza Corporation Stable therapeutic formulations
WO2007124411A1 (fr) 2006-04-20 2007-11-01 3M Innovative Properties Company Dispositif de pose d'une barrette de microaiguilles
JP5070764B2 (ja) * 2006-08-18 2012-11-14 凸版印刷株式会社 マイクロニードルのパッチの製造方法
JP4984736B2 (ja) * 2006-08-18 2012-07-25 凸版印刷株式会社 露光装置及び方法
WO2008076465A1 (fr) * 2006-12-21 2008-06-26 Primegen Biotech, Llc Puce à micro-injecteur
DE102009005003A1 (de) * 2008-08-01 2010-03-25 Frankenförder Forschungsgesellschaft mbH Vorrichtung und Verfahren für den mechanischen Ausschluss von Zellen

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4849355A (en) * 1985-01-08 1989-07-18 Wong Tai Kin Method of transferring genes into cells
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
US5262128A (en) * 1989-10-23 1993-11-16 The United States Of America As Represented By The Department Of Health And Human Services Array-type multiple cell injector
US5457041A (en) * 1994-03-25 1995-10-10 Science Applications International Corporation Needle array and method of introducing biological substances into living cells using the needle array

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO9610630A1 *

Also Published As

Publication number Publication date
CA2201315A1 (fr) 1996-04-11
WO1996010630A1 (fr) 1996-04-11
AU3758595A (en) 1996-04-26

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