CA2201315A1 - Introduction directe de substances etrangeres dans des cellules - Google Patents
Introduction directe de substances etrangeres dans des cellulesInfo
- Publication number
- CA2201315A1 CA2201315A1 CA 2201315 CA2201315A CA2201315A1 CA 2201315 A1 CA2201315 A1 CA 2201315A1 CA 2201315 CA2201315 CA 2201315 CA 2201315 A CA2201315 A CA 2201315A CA 2201315 A1 CA2201315 A1 CA 2201315A1
- Authority
- CA
- Canada
- Prior art keywords
- microprobes
- cells
- biological material
- support
- microns
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000000463 material Substances 0.000 title claims abstract description 23
- 210000004027 cell Anatomy 0.000 claims abstract description 120
- 238000000034 method Methods 0.000 claims abstract description 84
- 239000012620 biological material Substances 0.000 claims abstract description 59
- 229910052710 silicon Inorganic materials 0.000 claims abstract description 19
- 239000010703 silicon Substances 0.000 claims abstract description 19
- 238000005530 etching Methods 0.000 claims abstract description 17
- 239000007787 solid Substances 0.000 claims abstract description 14
- 210000000170 cell membrane Anatomy 0.000 claims abstract description 12
- 238000011065 in-situ storage Methods 0.000 claims abstract description 5
- 108020004414 DNA Proteins 0.000 claims description 27
- 108090000623 proteins and genes Proteins 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 241000244206 Nematoda Species 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 9
- 239000000126 substance Substances 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 241000588724 Escherichia coli Species 0.000 claims description 2
- 239000002178 crystalline material Substances 0.000 claims description 2
- 210000001339 epidermal cell Anatomy 0.000 claims description 2
- 210000004102 animal cell Anatomy 0.000 claims 3
- 102000053602 DNA Human genes 0.000 claims 2
- 108010004889 Heat-Shock Proteins Proteins 0.000 claims 1
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- 210000005260 human cell Anatomy 0.000 claims 1
- 239000008177 pharmaceutical agent Substances 0.000 claims 1
- 210000002421 cell wall Anatomy 0.000 abstract description 9
- 238000000338 in vitro Methods 0.000 abstract description 3
- 239000012528 membrane Substances 0.000 abstract description 2
- 235000012431 wafers Nutrition 0.000 description 35
- 229920002120 photoresistant polymer Polymers 0.000 description 20
- 239000000758 substrate Substances 0.000 description 18
- 241000196324 Embryophyta Species 0.000 description 17
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 14
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 13
- 239000000243 solution Substances 0.000 description 13
- 244000061176 Nicotiana tabacum Species 0.000 description 10
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 10
- 210000000172 cytosol Anatomy 0.000 description 9
- 239000000523 sample Substances 0.000 description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000002609 medium Substances 0.000 description 8
- 235000012239 silicon dioxide Nutrition 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 230000009466 transformation Effects 0.000 description 7
- KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 101100345589 Mus musculus Mical1 gene Proteins 0.000 description 6
- 239000010410 layer Substances 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 5
- 230000001404 mediated effect Effects 0.000 description 5
- 230000035515 penetration Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 239000000377 silicon dioxide Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 229910052581 Si3N4 Inorganic materials 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- ZAKOWWREFLAJOT-CEFNRUSXSA-N D-alpha-tocopherylacetate Chemical compound CC(=O)OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C ZAKOWWREFLAJOT-CEFNRUSXSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000002585 base Substances 0.000 description 3
- 230000010307 cell transformation Effects 0.000 description 3
- 229910052681 coesite Inorganic materials 0.000 description 3
- 229910052906 cristobalite Inorganic materials 0.000 description 3
- 230000004927 fusion Effects 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 239000002184 metal Substances 0.000 description 3
- 229910052751 metal Inorganic materials 0.000 description 3
- 238000000520 microinjection Methods 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 229910052682 stishovite Inorganic materials 0.000 description 3
- 229910052905 tridymite Inorganic materials 0.000 description 3
- 229910001868 water Inorganic materials 0.000 description 3
- -1 e.g. Substances 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 230000030833 cell death Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000012458 free base Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- FFUAGWLWBBFQJT-UHFFFAOYSA-N hexamethyldisilazane Chemical compound C[Si](C)(C)N[Si](C)(C)C FFUAGWLWBBFQJT-UHFFFAOYSA-N 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000010453 quartz Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 230000001052 transient effect Effects 0.000 description 2
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 206010068150 Acoustic shock Diseases 0.000 description 1
- 101100528916 Caenorhabditis elegans rol-6 gene Proteins 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 241000518994 Conta Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 235000002767 Daucus carota Nutrition 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 108010093031 Galactosidases Proteins 0.000 description 1
- GYHNNYVSQQEPJS-UHFFFAOYSA-N Gallium Chemical compound [Ga] GYHNNYVSQQEPJS-UHFFFAOYSA-N 0.000 description 1
- 102000053187 Glucuronidase Human genes 0.000 description 1
- 108010060309 Glucuronidase Proteins 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 241001527806 Iti Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- 241000341511 Nematodes Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940121363 anti-inflammatory agent Drugs 0.000 description 1
- 239000002260 anti-inflammatory agent Substances 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000000560 biocompatible material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 229920001821 foam rubber Polymers 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 229910052733 gallium Inorganic materials 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 101150054900 gus gene Proteins 0.000 description 1
- IXCSERBJSXMMFS-UHFFFAOYSA-N hcl hcl Chemical compound Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 description 1
- 230000010196 hermaphroditism Effects 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000005661 hydrophobic surface Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 238000007500 overflow downdraw method Methods 0.000 description 1
- 230000008823 permeabilization Effects 0.000 description 1
- 230000002399 phagocytotic effect Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 238000001020 plasma etching Methods 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 210000001938 protoplast Anatomy 0.000 description 1
- 235000021251 pulses Nutrition 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 101150080312 rol-6 gene Proteins 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 229910052814 silicon oxide Inorganic materials 0.000 description 1
- 229920002379 silicone rubber Polymers 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000009987 spinning Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 235000012976 tarts Nutrition 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000009279 wet oxidation reaction Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microinjection
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
- A01K67/0333—Genetically modified invertebrates, e.g. transgenic, polyploid
- A01K67/0335—Genetically modified worms
- A01K67/0336—Genetically modified Nematodes, e.g. Caenorhabditis elegans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43536—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms
- C07K14/4354—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from nematodes
- C07K14/43545—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from worms from nematodes from Caenorhabditis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Animal Behavior & Ethology (AREA)
- Sustainable Development (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La présente invention concerne un procédé simple, peu onéreux et précis permettant d'introduire une substance étrangère dans une population prédéterminée de cellules cibles. En un premier temps, le procédé consiste à disposer (a) des microsondes biologiquement inertes positionnées sur un support, (b) une masse solide ou quasi-solide de cellules cibles définissant une interface avec les microsondes, et (c) une substance biologique à l'interface. En un second temps, le procédé consiste à faire entrer les cellules en contact avec les microsondes, de sorte que les microsondes perforent de façon non létale les parois ou membranes cellulaires des cellules. Les microsondes, qui font de préférence corps avec le support, sont préparées par attaque chimique d'une plaquette unique d'un cristal telle que le silicium. La forme des microsondes est de préférence pyramidale. Le contact entre cellules cibles et microsondes peut se faire soit in vitro, soit in situ. Ce procédé convient avantageusement à presque tous les types de cellules et de substances biologiques susceptibles d'être introduites dans les cellules décrites ici.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US31533694A | 1994-09-30 | 1994-09-30 | |
| US08/315,336 | 1994-09-30 | ||
| US33460694A | 1994-11-04 | 1994-11-04 | |
| US08/334,606 | 1994-11-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2201315A1 true CA2201315A1 (fr) | 1996-04-11 |
Family
ID=26979842
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2201315 Abandoned CA2201315A1 (fr) | 1994-09-30 | 1995-09-29 | Introduction directe de substances etrangeres dans des cellules |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0782614A1 (fr) |
| AU (1) | AU3758595A (fr) |
| CA (1) | CA2201315A1 (fr) |
| WO (1) | WO1996010630A1 (fr) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9815819D0 (en) | 1998-07-22 | 1998-09-16 | Secr Defence | Transferring materials into cells and a microneedle array |
| ATE238412T1 (de) * | 1998-10-08 | 2003-05-15 | Astrazeneca Ab | Mikrofabrizierter zellinjektor |
| EP1333880B1 (fr) | 2000-10-26 | 2009-04-15 | Alza Corporation | Dispositifs d'administration de medicament transdermique avec des microprotrusions enrobees |
| ATE336277T1 (de) * | 2002-06-28 | 2006-09-15 | Alza Corp | Verfahren zur oberflächenbeschichtung von vorrichtungen zur transdermalen verabreichung von arzneien mit beschichteten mikrovorsprüngen |
| MXPA05000597A (es) * | 2002-07-19 | 2005-04-28 | 3M Innovative Properties Co | Dispositivos de microaguja y aparatos de administracion por microaguja. |
| JP5456234B2 (ja) | 2003-06-30 | 2014-03-26 | アルザ・コーポレーシヨン | 非揮発性の対イオンを含有する被覆された微小突起のための製剤 |
| AU2004268616B2 (en) | 2003-08-25 | 2010-10-07 | 3M Innovative Properties Company | Delivery of immune response modifier compounds |
| US7132242B2 (en) * | 2003-11-10 | 2006-11-07 | Hewlett-Packard Development Company, L.P. | Method and device for targeted delivery of materials to selected single cells |
| US7846488B2 (en) | 2004-11-18 | 2010-12-07 | 3M Innovative Properties Company | Masking method for coating a microneedle array |
| US8057842B2 (en) | 2004-11-18 | 2011-11-15 | 3M Innovative Properties Company | Method of contact coating a microneedle array |
| CA2587387C (fr) | 2004-11-18 | 2013-06-25 | 3M Innovative Properties Company | Procede d'enrobage d'une matrice de micro-aiguilles par mise en contact |
| US9174035B2 (en) | 2004-11-18 | 2015-11-03 | 3M Innovative Properties Company | Microneedle array applicator and retainer |
| EP2067599B1 (fr) | 2004-12-07 | 2016-10-26 | 3M Innovative Properties Company | Procédé de moulage d'une microaiguille |
| US10035008B2 (en) | 2005-04-07 | 2018-07-31 | 3M Innovative Properties Company | System and method for tool feedback sensing |
| EP1904158B1 (fr) | 2005-06-24 | 2013-07-24 | 3M Innovative Properties Company | Timbre repliable et son procede d'application |
| WO2007002521A2 (fr) | 2005-06-27 | 2007-01-04 | 3M Innovative Properties Company | Dispositif applicateur de matrice de micro-aiguilles et procede d'application d'une telle matrice |
| CA2613114C (fr) | 2005-06-27 | 2015-02-24 | 3M Innovative Properties Company | Ensemble cartouche a micro-aiguilles et son procede d'application |
| US8900180B2 (en) | 2005-11-18 | 2014-12-02 | 3M Innovative Properties Company | Coatable compositions, coatings derived therefrom and microarrays having such coatings |
| WO2007076458A1 (fr) * | 2005-12-21 | 2007-07-05 | Primegen Biotech Llc | Puce a micro-injecteur |
| WO2007084247A2 (fr) | 2005-12-28 | 2007-07-26 | Alza Corporation | Formulations therapeutiques stables |
| US9119945B2 (en) | 2006-04-20 | 2015-09-01 | 3M Innovative Properties Company | Device for applying a microneedle array |
| JP5070764B2 (ja) * | 2006-08-18 | 2012-11-14 | 凸版印刷株式会社 | マイクロニードルのパッチの製造方法 |
| JP4984736B2 (ja) * | 2006-08-18 | 2012-07-25 | 凸版印刷株式会社 | 露光装置及び方法 |
| WO2008076465A1 (fr) * | 2006-12-21 | 2008-06-26 | Primegen Biotech, Llc | Puce à micro-injecteur |
| DE102009005003A1 (de) * | 2008-08-01 | 2010-03-25 | Frankenförder Forschungsgesellschaft mbH | Vorrichtung und Verfahren für den mechanischen Ausschluss von Zellen |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4849355A (en) * | 1985-01-08 | 1989-07-18 | Wong Tai Kin | Method of transferring genes into cells |
| US5302523A (en) * | 1989-06-21 | 1994-04-12 | Zeneca Limited | Transformation of plant cells |
| US5262128A (en) * | 1989-10-23 | 1993-11-16 | The United States Of America As Represented By The Department Of Health And Human Services | Array-type multiple cell injector |
| US5457041A (en) * | 1994-03-25 | 1995-10-10 | Science Applications International Corporation | Needle array and method of introducing biological substances into living cells using the needle array |
-
1995
- 1995-09-29 EP EP95935656A patent/EP0782614A1/fr not_active Withdrawn
- 1995-09-29 AU AU37585/95A patent/AU3758595A/en not_active Abandoned
- 1995-09-29 WO PCT/US1995/012381 patent/WO1996010630A1/fr not_active Application Discontinuation
- 1995-09-29 CA CA 2201315 patent/CA2201315A1/fr not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1996010630A1 (fr) | 1996-04-11 |
| EP0782614A1 (fr) | 1997-07-09 |
| AU3758595A (en) | 1996-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2201315A1 (fr) | Introduction directe de substances etrangeres dans des cellules | |
| US5137817A (en) | Apparatus and method for electroporation | |
| US5457041A (en) | Needle array and method of introducing biological substances into living cells using the needle array | |
| Van West et al. | Oomycete plant pathogens use electric fields to target roots | |
| EP1522578B1 (fr) | Transfert de matières dans des cellules utilisant du silicium poreux | |
| US6686299B2 (en) | Nanosyringe array and method | |
| JP3035608B2 (ja) | マイクロキャピラリーアレイ、その製造方法、及び物質注入装置 | |
| EP0444882B1 (fr) | Procédé de transformation de plantes avec détection précoce des événements de transformation de la lignée germurale | |
| JP4842512B2 (ja) | 並列薬剤送達と細胞構造の電気穿孔法を併合した方法及びその使用 | |
| JP2002325572A (ja) | 外来物質の導入方法 | |
| US6563017B2 (en) | In vivo electroporation method for early stage embryo of chickens | |
| Trimmer et al. | Injection of DNA into plant and animal tissues with micromechanical piercing structures | |
| EP3563673A1 (fr) | Procédé d'introduction dune substance dans une plante | |
| JPH06343478A (ja) | マイクロインジェクション方法及び装置 | |
| JPH11511974A (ja) | エレクトロポレーションによって媒介される無傷の植物中への分子導入 | |
| US6617153B2 (en) | Portable magneto-mechanical delivery device and method for delivery of reactive substances | |
| CA2020265C (fr) | Methode et appareil d'acceleration de matiere propulsable | |
| O’ Brochta et al. | Transformation systems in insects | |
| JP2021151201A (ja) | マイクロニードルアレイ及びそれを用いた植物細胞への物質導入方法 | |
| JP4625925B2 (ja) | 細胞に対して物質を導入する方法 | |
| Banerjee et al. | Transient expression of β-glucuronidase in embryo axes of cotton by Agrobacterium and particle bombardment methods | |
| US20040182706A1 (en) | Microinjection method and device based on electroosmosis | |
| Paik et al. | A highly dense nanoneedle array for intracellular gene delivery | |
| Kościańska et al. | Electroporated intact BY-2 tobacco culture cells as a model of transient expression study. | |
| JP5201497B2 (ja) | 超音波形質転換法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Dead |