EP0691158B1 - Pipettiersystem - Google Patents

Pipettiersystem Download PDF

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Publication number
EP0691158B1
EP0691158B1 EP95108589A EP95108589A EP0691158B1 EP 0691158 B1 EP0691158 B1 EP 0691158B1 EP 95108589 A EP95108589 A EP 95108589A EP 95108589 A EP95108589 A EP 95108589A EP 0691158 B1 EP0691158 B1 EP 0691158B1
Authority
EP
European Patent Office
Prior art keywords
plunger
pipetting
exchange part
coding
cylinder
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP95108589A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP0691158A2 (de
EP0691158A3 (de
Inventor
Peter Mahler
Hubert Dr. Kunze
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Brand GmbH and Co KG
Original Assignee
Brand GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Brand GmbH and Co KG filed Critical Brand GmbH and Co KG
Priority to DE29522141U priority Critical patent/DE29522141U1/de
Publication of EP0691158A2 publication Critical patent/EP0691158A2/de
Publication of EP0691158A3 publication Critical patent/EP0691158A3/de
Application granted granted Critical
Publication of EP0691158B1 publication Critical patent/EP0691158B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0234—Repeating pipettes, i.e. for dispensing multiple doses from a single charge

Definitions

  • the invention relates to a pipetting system with a pipetting device and one at Pipetting device detachably attachable exchange part, which contains the liquid to be pipetted records, with the features of the preamble of claim 1.
  • Subject of the invention is also an exchange part for such a pipetting system.
  • each replacement part has a code for identification of its type.
  • This coding is in the known pipetting system by protrusions and gaps on the cylinder or for holding the replacement part in the Pipette device serving cylinder flange formed. This coding is from a Reading device, which is attached to the cylinder in the pipetting device, read, by mechanical scanning of the protrusions and gaps Switches or by inductive, capacitive or resistive data acquisition.
  • a pipetting system is known (US Pat. No. 3,757,585), in which a Pipettier réelle a detachably attachable replacement part for absorbing the liquid is provided.
  • This is an air cushion pipetting system, at which the pipetting device itself is designed as a piston-cylinder unit, but which too pipetting liquid does not enter the interior of the cylinder.
  • the exchange part is always the same for different pipetting devices, it is a disposable part. Different pipetting devices are provided for different volumes.
  • the different, hand-operated pipetting devices add coding Identification of their type, namely coding by color marks in two places, namely on the one hand on the cylinder by means of a cylinder ring, on the other hand on the piston of the pipetting device by means of a color marking on the piston head.
  • Identification of their type namely coding by color marks in two places, namely on the one hand on the cylinder by means of a cylinder ring, on the other hand on the piston of the pipetting device by means of a color marking on the piston head.
  • the teaching of the invention is based on the pipetting system explained at the beginning, the problem is based on the known pipetting system explained at the beginning To be designed so that the identification of the type of attached to the pipetting device Exchange part for each exchange part is possible without much effort.
  • the arrangement of the coding of the replacement part on the piston head is particularly preferred of the piston, i.e. where the piston actuator of the pipetting device is on Piston attacks.
  • a pipetting system with a pipetting device is detachable from the prior art attachable exchange part known (DE - A - 27 36 551), in which various Exchange parts of different types, but with the same relative position for all types are attachable by pistons and cylinders.
  • a piston actuator of the pipetting device detects the piston head of the piston of an exchange part on one Keyway. Below this piston head is on the piston rod of the piston a cylindrical, radial projection with an annular seal on the cylinder head of the exchange part cooperates. This lead serves in conjunction with the seal, a fluid tight seal for the piston of the Realize exchange part. Different diameters of pistons and protrusions on the piston for different types (volumes) of the replacement part beer realized. A coding of the replacement part to identify its type is not disclosed here.
  • the piston head is digitally encoded and it is done an optical detection of the coding.
  • the piston head is encoded in that it is transparent or opaque in sections. This enables the use of a simple one Binary codes.
  • the pipetting device has a reading unit with one or more Light barriers that illuminate the piston head and between transparent and distinguish opaque. Transparency and opacity are good permeability or poor permeability for the light of the light barriers in their specific Understand the wavelength range. This allows opacity and transparency realize that material or no material of the piston head is in the light path.
  • the light barriers work with infrared light
  • Each light barrier has an infrared light source, an infrared photo detector and a spectral filter upstream of this, the light essentially can only pass from the emission band of the light source.
  • the filter serves to suppress stray light and the improvement of the signal-background ratio.
  • the light barriers can work at different light wavelengths To prevent crosstalk from one light barrier to another. Accomplished the same one requires less equipment in that the light barriers are in chronological succession to be activated. The required speed of the measurement does not offer Problem, and you can get by with only one type of light source and photo detector.
  • the reading unit is always attached an exchange part on the pipetting device z. B. by switching at short notice put into operation.
  • the switching process can, for example, with the snap be connected to a closure element.
  • the reading unit becomes well-defined Active times when an identification of replacement parts makes sense and is clear Compared to a permanent operation of the reading unit, the result is Advantage of lower energy consumption, which is particularly true for battery and accumulator-operated Manual pipetting devices is important. Also need briefly occurring undefined states z. B. when attaching or detaching one Exchange part not to be recognized and intercepted by the electronics.
  • the reading unit is to be self-checked suitable. This refers to the functionality of all light barriers and the fact that their light path is not faulty, e.g. B. by a foreign body, a movable closure element or the like.
  • the self-check is preferably initiated automatically.
  • the piston actuator goes with the piston head a removable part to be attached to the pipetting device on.
  • the reading unit is suitable for detecting the coding on the piston head, during its connection to the piston actuator and the piston completely has moved into the cylinder of the replacement part
  • the piston head is essentially cylindrical. It has one or more circumferential radial projections, one of which is closed the gripper associated with the piston actuator.
  • the piston head is through the axial Position of the protrusion or protrusions and next to and / or in between gaps are encoded.
  • n projections of which several may also form longer, integrally connected blocks, and n light barriers. Transparency on all light barriers is assigned to the self-check.
  • the invention relates to the coded exchange parts in the form of piston-cylinder units for a pipetting system of the type described.
  • these are exchange parts, the piston head of which is binary coded by the fact that it sectionally transparent for the light of one or more light barriers or is opaque, preferably with the omission of those used for the self-check binary everything one is immediately completely transparent.
  • the piston head is for the purpose of foreign part detection moreover at one point transparent to the light of the light barrier (s) which are the replacement parts of all other manufacturers for the light opaque.
  • the pipetting device is a repeating pipetting device for repeated output of presettable, preferably the same Volumes of liquid and / or around a pipetting device designed as a hand-held device, in particular one in which the pipetting movement is carried out directly by hand performed by an operator.
  • the pipetting device 40 includes exchange parts 10 in the form of Syringes that come in seven different sizes.
  • the one shown in Fig. 1 and Fig. 2 Syringe 10 is selected as an example.
  • the terms syringe and replacement part are to be understood synonymously.
  • the syringe 10 has a hollow body in the form of a circular cylinder 12 which is open at the top is. At the upper edge of the cylinder 12 is a circumferential, radially outward protruding collar 14.
  • the cylinder 12 goes into a central and axially attached hollow tip 16 over that is conical and tapered down.
  • the hollow tip 16 has a smaller one Diameter than the cylinder 12, so that at the bottom 18 a diameter step is present.
  • the interior of the hollow tip 16 stands over a central bottom opening of the Cylinder 12 with its cylinder interior in connection.
  • the lower opening 20 of the Hollow tip 16 serves as a suction and discharge opening for liquid to be pipetted.
  • a piston 22 is accommodated in the cylinder interior of the syringe 10 under sealing, the fully retracted into the cylinder 12 in the drawings and in plant with the bottom 18 is shown.
  • the piston 22 has a piston rod 24 with a Piston head 38, which in the illustrated embodiment from the opening of the Cylinder 12 protrudes upward, but also in whole or in part in the cylinder 12 can lie sunk.
  • the piston 22 forms a single-acting with the cylinder 12 Piston-cylinder unit.
  • the piston head 38 is circular cylindrical and graduated in diameter. He has one central cylindrical shaft 26 and one or more integrally formed therewith, circumferential radial projections 28.
  • the projections 28 are of radial planes 30 and outer cylinder jacket surfaces 32 limited in the axial direction swear.
  • On shaft sections next to and between the projections 28 radial gaps 34 adjacent the projections 28.
  • the shaft end is at the top 36 of the piston head 38 so that there is always a radial gap 34 here.
  • the pipetting device 40 has a housing with a receptacle for a syringe 10 which is about a side insertion opening is accessible. Collar 14 and piston head 38 of the Syringe 10 fit through the insertion opening into the receptacle when the plunger 22 is fully retracted into the cylinder 12. The collar 14 has when inserted Guide on both sides, inwardly converging ramps of the housing.
  • the cylinder 12 of the syringe 10 is firmly positioned with the collar 14 in the receptacle.
  • the piston 22 can be operated by means of a piston actuator 42 which is attached to the piston head 38 engages, adjust in an axial pipetting movement relative to the cylinder 12.
  • the piston member 42 has a gripper with two gripper jaws lying opposite one another 44, between which the piston head 38 fits.
  • the cheek profile is partially cylindrical and the outer diameter of the projections on the piston head 38 28 adjusted
  • the gripper is open on the insert side.
  • When inserting a syringe 10 into the pipetting device 40 are the gripper jaws 44 by the piston head entering between them 38 deflected and spread elastically.
  • the gripper jaws 44 lie around the piston head 38 and snap firmly to it
  • the piston head 38 is on the cylinder jacket the projections 28 held non-positively between the gripper jaws 44. Insert side the projections 28 of the piston head 38 protrude from the gripper (cf. Fig. 2).
  • the cylinder diameters are different from type to type.
  • the syringes are otherwise identical and especially the same length.
  • the respective type of syringe 10 is based on the protrusions 28 of the piston head 38 binary coded.
  • a grid dimension of the coding is the smallest axial length of a projection 28 Piston head 38 is over six times this length with projections 28 and Lükken 34 encoded next to and in between.
  • the grid position 1 at the top of the piston head is associated with the above shaft end 36, so that there is always a Gap 34 is located.
  • At each of the five grid positions 2 to 6 is located either a protrusion 28 or a gap 34.
  • each light barrier 46 - 52 belongs to an infrared photodiode 54 as a light source on one side and an opposite infrared photodetector 56 on the other side of the Piston head 38.
  • the light barriers 46-52 illuminate the piston head 38 when it is at Piston 22 fully retracted into cylinder 12 in the gripper of the piston actuator 42 is located.
  • the beam path of the light barriers 46 - 52 runs in parallel in central radial planes, which are axially offset from each other.
  • a projection 28 of the Piston head 38 is on its periphery protruding from the gripper hit by the infrared light secant and the beam path of the light barrier 46 - 52 interrupted.
  • the radial gaps 34 formed next to or between the projections 28 have the Light barriers 46 - 52 passage.
  • a first light barrier 46 in grid position 1 shines through the diameter return - Gap 34 - at the upper shaft end 36 of the piston head 38.
  • This light barrier 46 is used to detect foreign syringes, all of which are massive and for the infrared light is opaque.
  • Transparency in raster position 1 the first bit digital one.
  • the three light barriers 48 - 52 used to read out the type coding illuminate the piston head 38 at grid position 2 above, 4 center and 6 below.
  • This Photoelectric sensors 46, 50, 52 are therefore spaced apart from one another by twice the grid dimension, whereas the two light barriers 46, 48 are at a distance at the very top have simple grid dimensions.
  • the piston heads 38 on the three light barriers 48 - 52 of the type code reading unit either transparent (gap 34; digital One) or opaque (projection 28; digital zero).
  • the syringes 10 are as in Fig. 4 and 5 can be seen with the binary numbers 000, i. H. Projection 28 on all light barriers (see also FIGS. 1 to 3) to 110, i. H. Gaps 34 on the two light barriers 48, 50 and projection 28 on the light barrier 52 type-coded.
  • the binary number 111, d. H. Gap 34 on all three light barriers 48 - 52 is not assigned as a type code, because light passage at all light barriers 46 - 52 for a self-check is occupied.
  • the axial length corresponds to the projections 28 the simple, double or triple grid dimension.
  • the sum of the axial length the protrusions 28 are the same for all syringes 10, namely three times Pitch. The result is an identical frictional connection on the gripper for all pistons 22.
  • the replacement part 10 suitable for the pipetting system according to the invention is drawn is characterized by a coding on the piston head 38 of the piston 22.
  • a coding on the piston head 38 of the piston 22 is provided according to a particularly preferred teaching that the piston head 38 to form the encoding a plurality of circumferential radial projections 28 and several circumferential radial ones located next to and / or in between Gaps 34, which together form the coding. This is particularly shown in Fig. 3 Good.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Pipeline Systems (AREA)
  • Sampling And Sample Adjustment (AREA)
EP95108589A 1994-07-07 1995-06-06 Pipettiersystem Expired - Lifetime EP0691158B1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE29522141U DE29522141U1 (de) 1994-07-07 1995-06-06 Pipettiersystem

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE4423528 1994-07-07
DE44235283F 1994-07-07
DE44235283 1994-07-07

Publications (3)

Publication Number Publication Date
EP0691158A2 EP0691158A2 (de) 1996-01-10
EP0691158A3 EP0691158A3 (de) 1997-01-08
EP0691158B1 true EP0691158B1 (de) 2001-11-07

Family

ID=6522288

Family Applications (1)

Application Number Title Priority Date Filing Date
EP95108589A Expired - Lifetime EP0691158B1 (de) 1994-07-07 1995-06-06 Pipettiersystem

Country Status (5)

Country Link
EP (1) EP0691158B1 (pl)
DE (1) DE59509794D1 (pl)
ES (1) ES2163459T3 (pl)
FI (1) FI109406B (pl)
PL (2) PL178640B1 (pl)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2574402A1 (de) 2011-09-30 2013-04-03 Eppendorf Ag Spritze mit Spritzenzylinder mit Kodierung und Prüfelementen
EP3831487A1 (de) 2019-12-06 2021-06-09 Eppendorf AG Pipette für den gebrauch mit einer pipettenspitze und pipettenspitze für den gebrauch mit einer pipette
EP4149682A1 (en) * 2019-10-25 2023-03-22 Mettler-Toledo Rainin, LLC Positive displacement pipette syringe identification system

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19915066C2 (de) 1999-04-01 2001-09-13 Brand Gmbh & Co Kg Verfahren zur Erkennung des Typs von austauschbaren gerätespezifischen Kolben-Zylinder-Einheiten für Pipettier- oder Dosiergeräte sowie Pipettier- und Dosiersystem
DE19915771C1 (de) * 1999-04-08 2001-01-04 Retec Elektronische Regeltechn System zum Aufnehmen und Abgeben von Fluidvolumina
DE19948818C2 (de) * 1999-10-09 2002-09-05 Retec Elektronische Regeltechn System zum Aufnehmen und Abgeben von Fluidvolumina
DE10001938C2 (de) * 2000-01-19 2002-08-01 Retec Elektronische Regeltechn System zum Aufnehmen und Abgeben von Fluidvolumina und Verwendung des Systems
DE10022150A1 (de) * 2000-05-08 2001-11-15 Roland Manowski Magnetkolbenmotor und Verfahren zu dessen Herstellung
PL357692A1 (pl) * 2002-12-11 2004-06-14 P.Z.HTL Spółka Akcyjna Sposób odczytu parametrów wymiennych trzonów w pipecie i pipeta z odczytem parametrów wymiennych trzonu
DE102006009816A1 (de) 2006-02-28 2007-09-06 Eppendorf Ag System und Verfahren zum Titrieren von Flüssigkeiten
DE102009016590A1 (de) * 2009-03-31 2010-10-07 Eppendorf Ag Dosiervorrichtung
US9291529B2 (en) 2011-09-30 2016-03-22 Eppendorf Ag Syringe for use with a metering device
DE202017101007U1 (de) 2017-02-23 2018-05-24 Brand Gmbh + Co Kg Austauschbare Kolben-Zylinder-Einheit für einen Dispenser, Dispenser und System zum Aufnehmen und Abgeben von Fluidvolumina
USD926224S1 (en) 2018-10-11 2021-07-27 Brand Gmbh + Co Kg Laboratory device display screen with graphical user interface
US11389792B2 (en) 2019-10-25 2022-07-19 Mettler-Toledo Rainin, LLC Syringe for powered positive displacement pipette
US11446672B2 (en) * 2019-10-25 2022-09-20 Mettler-Toledo Rainin, LLC Powered positive displacement pipette syringe piston grasping mechanism
US11471878B2 (en) 2019-10-25 2022-10-18 Mettler-Toledo Rainin, LLC Powered positive displacement pipette
US11369954B2 (en) 2019-10-25 2022-06-28 Mettler-Toledo Rainin, LLC Powered positive displacement pipette assembly
EP3831486A1 (de) * 2019-12-06 2021-06-09 Eppendorf AG Pipettenspitzenfamilie umfassend pipettenspitzen für den gebrauch mit pipetten einer pipettenfamilie und pipettenfamilie umfassend pipetten für den gebrauch mit pipettenspitzen einer pipettenspitzenfamilie
CA3165085C (en) * 2020-02-14 2024-02-20 DeNovix, Inc. Dynamic broad volumetric range pipette
US12472491B2 (en) 2022-06-28 2025-11-18 Mettler-Toledo Rainin, LLC Positive displacement pipette syringe with reduced fluid drag

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4563907A (en) * 1983-10-31 1986-01-14 Micromedic Systems Inc. Direct reading automatic pipette

Family Cites Families (9)

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US3757585A (en) * 1968-08-16 1973-09-11 Heller Labor Pipette apparatus
US4099548A (en) * 1976-08-25 1978-07-11 Oxford Laboratories Inc. Hand-held pipette for repetitively dispensing precise volumes of liquid
DE2914176A1 (de) * 1979-04-07 1980-10-16 Kernforschungsz Karlsruhe Einrichtung zum identifizieren eines transportbehaelters
US4489618A (en) * 1980-07-03 1984-12-25 Meyer Richard C Pipet signalling system
US4569662A (en) * 1981-10-26 1986-02-11 Dragan William B Bulk cartridge for packaging and dispensing a dental material
FI852704L (fi) 1985-07-08 1987-01-09 Labsystems Oy Eldriven pipett.
US4988481A (en) 1989-01-30 1991-01-29 Labsystems Oy Electrical pipette
US4978335A (en) 1989-09-29 1990-12-18 Medex, Inc. Infusion pump with bar code input to computer
FI922939A0 (fi) 1992-06-24 1992-06-24 Labsystems Oy Knappipett.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4563907A (en) * 1983-10-31 1986-01-14 Micromedic Systems Inc. Direct reading automatic pipette

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2574402A1 (de) 2011-09-30 2013-04-03 Eppendorf Ag Spritze mit Spritzenzylinder mit Kodierung und Prüfelementen
WO2013045013A1 (de) 2011-09-30 2013-04-04 Eppendorf Ag Spritze mit spritzenzylinder mit kodierung und prüfelementen
EP4149682A1 (en) * 2019-10-25 2023-03-22 Mettler-Toledo Rainin, LLC Positive displacement pipette syringe identification system
EP3831487A1 (de) 2019-12-06 2021-06-09 Eppendorf AG Pipette für den gebrauch mit einer pipettenspitze und pipettenspitze für den gebrauch mit einer pipette

Also Published As

Publication number Publication date
FI953328A0 (fi) 1995-07-06
PL178640B1 (pl) 2000-05-31
PL179567B1 (pl) 2000-09-29
PL309166A1 (en) 1996-01-08
ES2163459T3 (es) 2002-02-01
EP0691158A2 (de) 1996-01-10
EP0691158A3 (de) 1997-01-08
FI109406B (fi) 2002-07-31
FI953328L (fi) 1996-01-08
DE59509794D1 (de) 2001-12-13

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