EP0594776A1 - Plasmid-replikationsursprung, welcher die kopienzahl von ihn enthaltenden plasmiden erhöht - Google Patents
Plasmid-replikationsursprung, welcher die kopienzahl von ihn enthaltenden plasmiden erhöhtInfo
- Publication number
- EP0594776A1 EP0594776A1 EP92916263A EP92916263A EP0594776A1 EP 0594776 A1 EP0594776 A1 EP 0594776A1 EP 92916263 A EP92916263 A EP 92916263A EP 92916263 A EP92916263 A EP 92916263A EP 0594776 A1 EP0594776 A1 EP 0594776A1
- Authority
- EP
- European Patent Office
- Prior art keywords
- plasmid
- origin
- plasmids
- replication
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR9108836 | 1991-07-12 | ||
FR9108836A FR2678947B1 (fr) | 1991-07-12 | 1991-07-12 | Origine de replication plasmidique augmentant le nombre de copies du plasmide comprenant ladite origine. |
PCT/FR1992/000671 WO1993001293A1 (fr) | 1991-07-12 | 1992-07-10 | Origine de replication plasmidique augmentant le nombre de copies du plasmide comprenant ladite origine |
Publications (1)
Publication Number | Publication Date |
---|---|
EP0594776A1 true EP0594776A1 (de) | 1994-05-04 |
Family
ID=9415055
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP92916263A Withdrawn EP0594776A1 (de) | 1991-07-12 | 1992-07-10 | Plasmid-replikationsursprung, welcher die kopienzahl von ihn enthaltenden plasmiden erhöht |
Country Status (4)
Country | Link |
---|---|
US (1) | US5565333A (de) |
EP (1) | EP0594776A1 (de) |
FR (1) | FR2678947B1 (de) |
WO (1) | WO1993001293A1 (de) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9605453D0 (en) | 1996-03-15 | 1996-05-15 | Univ Cambridge Tech | Cultured cells |
JP4733298B2 (ja) * | 2001-07-05 | 2011-07-27 | 三菱レイヨン株式会社 | プラスミドの自律増殖に関する機能を有する遺伝子を含むdna断片 |
US7247467B2 (en) * | 2003-09-17 | 2007-07-24 | E. I. Du Pont De Nemours And Company | Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number |
WO2007035323A1 (en) * | 2005-09-16 | 2007-03-29 | Monsanto Technology Llc | Hybrid portable origin of replication plasmids |
WO2009036095A1 (en) * | 2007-09-10 | 2009-03-19 | Joule Biotechnologies, Inc. | Engineered light-harvesting organisms |
EP2217695A2 (de) * | 2007-11-10 | 2010-08-18 | Joule Unlimited, Inc. | Hyperfotosynthetische organismen |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA1209501A (en) * | 1982-09-16 | 1986-08-12 | Nikos Panayotatos | Expression vector |
-
1991
- 1991-07-12 FR FR9108836A patent/FR2678947B1/fr not_active Expired - Fee Related
-
1992
- 1992-07-10 WO PCT/FR1992/000671 patent/WO1993001293A1/fr not_active Application Discontinuation
- 1992-07-10 EP EP92916263A patent/EP0594776A1/de not_active Withdrawn
- 1992-07-10 US US08/175,354 patent/US5565333A/en not_active Expired - Fee Related
Non-Patent Citations (1)
Title |
---|
See references of WO9301293A1 * |
Also Published As
Publication number | Publication date |
---|---|
US5565333A (en) | 1996-10-15 |
WO1993001293A1 (fr) | 1993-01-21 |
FR2678947A1 (fr) | 1993-01-15 |
FR2678947B1 (fr) | 1993-11-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1366176B1 (de) | Cpg-freie synthetische gene und bakterielle plasmide | |
JP4361971B2 (ja) | 改変tn5トランスポザーゼを使用したインビトロ転位用システム | |
EP0011562B1 (de) | Mit einem Plasmid transformierte Saccharomyces cer. | |
Tegova et al. | Involvement of error-prone DNA polymerase IV in stationary-phase mutagenesis in Pseudomonas putida | |
EP0236210A1 (de) | Verfahren zur Herstellung von reifem, menschlichem Serum-Albumin | |
FR2500847A1 (fr) | Marqueurs genetiques selectifs pour cellules eucaryotes, procede de mise en oeuvre de tels marqueurs et application des cellules contenant un tel marqueur a la fabrication de proteines determinees apres leur transformation par un adn correspondant | |
EP0749489B1 (de) | Expressionskassette, die einen aus dem Schizosaccharomyces pombe-Pho4-Gene stammenden Promotor enthält | |
CA2285041C (fr) | Cassette de transformation de la levure | |
EP0012078B1 (de) | Vector, der die Einfügung eines prokaryotischen oder eukaryotischen Gens und die Ausscheidung des exprimierten Proteins gestattet | |
CA2197804C (fr) | Procede de production de proteines recombinantes, plasmides et cellules modifiees | |
EP0594776A1 (de) | Plasmid-replikationsursprung, welcher die kopienzahl von ihn enthaltenden plasmiden erhöht | |
FR3110575A1 (fr) | utilisation d’une protéine de fusion pour induire des modifications génétiques par recombinaison méiotique ciblée | |
KR100229152B1 (ko) | 점액세균의 유전자 조작방법 | |
Winckler et al. | Gene function analysis by amber stop codon suppression: CMBF is a nuclear protein that supports growth and development of Dictyostelium amoebae | |
KR20180128864A (ko) | 매칭된 5' 뉴클레오타이드를 포함하는 가이드 rna를 포함하는 유전자 교정용 조성물 및 이를 이용한 유전자 교정 방법 | |
EP1315822A2 (de) | Expressionsvektoren mit mutiertem tryptophanoperon-fragment | |
EP1124983B1 (de) | In vivo-verfahren zur herstellung von proteinen, die nicht-konventionelle aminosäuren enthalten | |
CA2077347C (fr) | Vecteurs de clonage et/ou d'expression, procede de preparation et leur utilisation | |
EP0149936A2 (de) | Mutanten von Escherichia-Coli, fähig zur Ausscheidung von periplasmatischen Proteinen in das extrazellulare Medium, deren Erhaltung und Verwendung bei der Herstellung von Beta-Laktamasen oder von Hybridproteinen von Beta-Laktamasen | |
EP1280890A1 (de) | Mutantenstämme geeignet zur herstellung von chemisch unterschiedlichen proteinen, die nicht-konventionelle aminosäuren enthalten | |
EP1071798A1 (de) | Neue vektoren zur kontrollierter expression von rekombinanten genen in prokaryontischen zellen | |
FR3127946A1 (fr) | Procédé de purification d’une protéine d’intérêt et moyens pour sa mise en œuvre | |
WO2004106526A1 (fr) | Vecteur de clonage d'insert a extremites franches permettant la selection positive des clones recombinants, methode de clonage dans ledit vecteur, utilisation dudit vecteur pour l'expression d'un insert | |
WO2003068978A2 (fr) | Combinaisons de vecteurs et leurs hotes pour propager et exprimer des genes recombinants sans recourir a des antibiotiques | |
CA2187170A1 (fr) | Procede de selection de cellules eucaryotes transformees et cellules obtenues |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
17P | Request for examination filed |
Effective date: 19931206 |
|
AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE CH DE DK ES FR GB GR IT LI LU MC NL SE |
|
RAP1 | Party data changed (applicant data changed or rights of an application transferred) |
Owner name: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNR Owner name: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE (INR |
|
GRAG | Despatch of communication of intention to grant |
Free format text: ORIGINAL CODE: EPIDOS AGRA |
|
17Q | First examination report despatched |
Effective date: 19960916 |
|
GRAH | Despatch of communication of intention to grant a patent |
Free format text: ORIGINAL CODE: EPIDOS IGRA |
|
STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
18D | Application deemed to be withdrawn |
Effective date: 19970620 |