Classifications

• • C—CHEMISTRY; METALLURGY
  • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
  • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
  • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
  • C11D3/16—Organic compounds
  • C11D3/38—Products with no well-defined composition, e.g. natural products
  • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
  • C11D3/38663—Stabilised liquid enzyme compositions

Definitions

• the present invention relates to detergent compositions comprising a lipolytic enzyme.
• the present invention relates to liquid detergent compositions comprising a lipolytic enzyme.
• a problem in using lipolytic enzymes in detergent compositions is often the occurrence of enzyme instability, leading to a reduction in enzyme activity upon use of the compositions, for example in the washing of fabrics. These instability problems are especially apparent in detergent compositions comprising lipolytic enzymes in combination with proteolytic enzymes.
• a stabiliser-lipolytic enzyme complex prevents the destabilisation reaction i.e between lipolytic enzymes and proteolytic enzymes and/or improves the resistance against denaturation and against proteolytic breakdown of the lipolytic enzyme, such that the half-life time of the lipase in the composition at 37°C is increased by a least 25 %, more preferably at least 50 %, most preferably at least 100 %.
• the half-life time of the lipolytic enzyme is the time-period starting at the moment of preparation of the detergent formulation until the moment that only 50% of the enzyme- activity is left.
• the stabilisation reaction is an inhibitory reaction, whereby the stabiliser- lipolytic enzyme complex is inactivated.
• the reversibility of complex formation can generally be effected by adding the stabilisation material in a concentration of from 2 to 100 times the K ; , wherein the K ; is the concentration at which 50 % of the lipase enzymes have formed the complex. More preferably the concentration is from 5-25 times the K ; , most preferably from 8 to 15 times.
• the detergent composition will generally be diluted 50 to 500 times, especially about 100 times, providing a concentration of stabiliser such that the stabiliser-lipolytic enzyme complex will be dissociated, thus providing active lipolytic enzymes in the wash liquor.
• the present invention relates to a detergent composition
• a detergent composition comprising a lipolytic enzyme and a stabilising material capable of reversibly forming a complex with the active site of the lipolytic enzyme, therewith increasing the half-lifetime of the lipase material.
• the stabiliser material is added to the detergent composition in a concentration of from 2 to 100 times the K ; .
• any material capable of reversible forming a complex with the active site of the lipolytic enzyme may be used.
• boronic acid materials are used.
• a second embodiment of the invention relates to a detergent composition
• a detergent composition comprising a lipolytic enzyme in combination with one or more boronic acid derivatives of formula I as indicated above.
• the stabilisation of the lipase enzyme by the boronic acid derivatives works via an inhibitory mechanism, whereby the boronic acid derivative reacts with the active site of the lipolytic enzyme, therewith protecting the enzyme against destabilisation.
• a dilution step will take place, for example by the addition of the detergent composition to the wash liquor. It is believed that due to this dilution, generally the protective boronic acid group will be detached form the active site of the lipolytic enzyme, therewith providing an active enzyme material under use conditions.
• Preferred boronic acid derivatives are of the formula I, whereby at least of the groups R 2 and R 3 is -OH, most preferably groups R 2 and R 3 both are -OH or one of these groups is -OH and the other is a C 1 - 8 alkyl or alkenyl group.
• boronic acid derivatives are of the following formula: wherein:
• the level of boronic acid derivatives in the detergent compositions according to the present invention may be varied within a broad range, dependant on the level of lipolytic enzyme and the reactivity of the boronic acid derivative. Generally the level of boronic acid derivatives will be from 0.00001 to 3 % by weight of the composition, more preferred from 0.0001 to 1 %, most preferred from 0.001 to 0.5 %.
• the added amount of lipolytic enzyme in the composition is preferably from 50-30,000 LU/g of detergent composition, whereby LU (lipase units) are defined as they are in EP 258 068.
• the level of lipolytic enzymes is often at least 100 LU/g, very usefully at least 250 LU/g, preferably less than 5000 LU/g, more preferably less than 2000 LU/g or less than 1000 LU/g.
• the lipolytic enzyme can be chosen from among a wide range of lipases: in particular the lipases described in for example the following patent specifications, EP 214 761 (NOVO), EP 258 068 (NOVO) and EP 305 216 (NOVO), and especially lipolytic enzymes showing immunological cross-reactivity with antisera raised against lipases from Thermomyces lanuginosus ATCC 22070, EP 205 208 (UNILEVER) and EP 206 390 (UNILEVER), and especially lipases showing immunological cross-reactivity with antisera raised against lipase from Chromobacter viscosum var lipolyticum NRRL B-3673 , or against lipase from Alcaligenes PL-679, ATCC 31371 and FERM-P 3783, also the lipases described in specifications WO 87/00859 (Gist-Brocades), WO 89/09263 (Gist Brocades), EP 331 376 (AMANO), DE 39 08 131 (
• Suitable in particular are for example the following commercially available lipase preparations: Novo Lipolase, Amano Lipase CE, P, B, AP, M-AP, AML and CES, and Meito lipases MY-30, OF, and PL, also esterase MM. lipozym, SP 225, SP 285, Siaken lipase, Enzeco lipase, Toyo Jozo lipase and Diosynth lipase.
• These commercially available enzymes are preferably used at levels of from 0.01 to 10 % by weight of the detergent composition, more preferred 0.05 to 5 %, most preferred 0.1 to 2 %, whereby the levels relate to the enzyme preparation in the form as supplied.
• lipase enzymes produced by genetic engineering may be used, such as for example described in WO 89-09263 (Gist-Brocades) and EP 218 272 (Gist-Brocades) as well as EP 258 068 (Novo) and EP 305 216 (Novo).
• boronic acid derivatives for the stabilisation of lipolytic enzymes is especially advantageous for detergent compositions comprising lipases in combination with proteolytic enzymes.
• Protease can preferably be included in the composition in amounts of the order of about 1 to 100 GU/mg detergent composition. Preferably the amount ranges from 2-50 and more preferably from 5 to 20 GU/mg.
• a GU is a Glycine unit, defined as the proteolytic enzyme activity which, under standard conditions, during a 15 minute incubation at 40 ° C, with N-acetyl casein as substrate, produces an amount of NH 2 groups equivalent to 1 micromole of glycine.
• a preferred example of a protease enzyme which may be used in compositions according to the invention is the subtilisin variety sold as Savinase (TM of Novo-Nordisk A/S) or Maxacal (TM of Gist-Brocades/IBIS) or as Opticlean (ex MKC) or AP122 (ex Showa Denko).
• Other useful examples of proteases include Maxatase, Esperase, Alcalase, proteinase K and subtilisin BPN', or variants obtained by enzyme engineering.
• proteolytic enzymes are preferably used at a level of from 0.01 to 10 % by weight of the composition, more preferably 0.05 to 2 %, most preferred 0.1 to 2 %, whereby the levels relate to the enzyme preparation in the form as supplied.
• Protease with a high isoelectric point have been found more stable under the conditions generally encountered in detergent compositions of the invention. Particularly good results have been obtained while using a high pl protease (ie pl above 9, say about 10) in a liquid detergent composition of pH less than about 9.
• Detergent compositions of the invention may take any suitable physical form such as powders, pastes, tablets and liquids.
• a preferred embodiment of the present invention relates to liquid detergent compositions, in particular liquid detergent compositions comprising an aqueous phase.
• compositions of the present invention also comprise detergent active materials.
• the detergent active materials in general, may comprise one or more surfactants, and may be selected from anionic, cationic, nonionic, zwitterionic and amphoteric species, and (provided mutually compatible) mixtures thereof.
• they may be chosen from any of the classes, sub-classes and specific materials described in "Surface Active Agents” Vol. I, by Schwartz & Perry, Interscience 1949 and "Surface Active Agents" Vol.
• Suitable nonionic surfactants include, in particular, the reaction products of compounds having a hydrophobic group and a reactive hydrogen atom, for example aliphatic alcohols, acids, amides or alkyl phenols with alkylene oxides, especially ethylene oxide either alone or with propylene oxide.
• Specific nonionic detergent compounds are alkyl (C 6 -C, 8 ) primary or secondary linear or branched alcohols with ethylene oxide, and products made by condensation of ethylene oxide with the reaction products of propylene oxide and ethylenediamine.
• Other so-called nonionic detergent compounds include long chain tertiary amine oxides, long chain tertiary phosphine oxides and dialkyl sulphoxides.
• salting out resistant active materials such as for example described in EP 328 177,
• alkyl poly glycoside surfactants such as for example disclosed in EP 70 074.
• Suitable anionic surfactants are usually water-soluble alkali metal salts of organic sulphates and sulphonates having alkyl radicals containing from about 8 to about 22 carbon atoms, the term alkyl being used to include the alkyl portion of higher acyl radicals.
• suitable synthetic anionic detergent compounds are sodium and potassium alkyl sulphates, especially those obtained by sulphating higher (C 8 -C 18 ) alcohols produced for example from tallow or coconut oil, sodium and potassium alkyl (C s -C 2o ) benzene sulphonates, particularly sodium linear secondary alkyl (C 10 -C 15 ) benzene sulphonates; sodium alkyl glyceryl ether sulphates, especially those ethers of the higher alcohols derived from tallow or coconut oil and synthetic alcohols derived from petroleum; sodium coconut oil fatty monoglyceride sulphates and sulphonates; sodium and potassium salts of sulphuric acid esters of higher (C 8 -C l8 ) fatty alcohol-alkylene oxide, particularly ethylene oxide, reaction products; the reaction products of fatty acids such as coconut fatty acids esterified with isethionic acid and neutralised with sodium hydroxide; sodium and potassium salts of fatty acid amide
• an alkali metal soap of a fatty acid especially a soap of an acid having from 12 to 18 carbon atoms, for example oleic acid, ricinoleic acid, and fatty acids derived from castor oil, alkylsuccinic acid, rapeseed oil, groundnut oil, coconut oil, palmkernel oil or mixtures thereof.
• the sodium or potassium soaps of these acids can be used.
• the total detergent active material may be present at from 2% to 60% by weight of the total composition, for example from 5% to 50% and typically from 10% to 40% by weight.
• one preferred class of compositions comprises at least 20%, most preferably at least 25% and especially at least 30% of detergent active material based on the weight of the total composition.
• Liquid detergent compositions of the invention may be un-structured (isotropic) or structured.
• Structured liquids of the invention may be internally structured whereby the structure is formed by the detergent active materials in the composition or externally structured, whereby the structure is provided by an external structurant.
• compositions of the invention are internally structured.
• compositions of the invention comprise a structure of lamellar droplets comprising surfactant materials.
• lamellar droplets are called "spherulites".
• the presence and identity of a surfactant structuring system in a liquid may be determined by means known to those skilled in the art for example, optical techniques, various rheometrical measurements, x-ray or neutron diffraction, and sometimes, electron microscopy.
• the aqueous continuous phase contains dissolved electrolyte.
• electrolyte means any ionic water soluble material.
• the electrolytes may be used, with one or more of the electrolytes being in the dissolved aqueous phase and one or more being substantially only in the suspended solid phase.
• Two or more electrolytes may also be distributed approximately proportionally, between these two phases. In part, this may depend on processing, e.g.
• salts includes all organic and inorganic materials which may be included, other than surfactants and water, whether or not they are ionic, and this term encompasses the sub-set of the electrolytes (water soluble materials).
• compositions where the detergent active material comprises blends of different surfactant types.
• Typical blends useful for fabric washing compositions include those where the primary surfactant(s) comprise nonionic and/or a non-alkoxylated anionic and/or an alkoxylated anionic surfactant.
• the compositions contain from 0% to 60%, especially from 1- 60 %, more preferably 10 to 45% of a salting-out electrolyte.
• Salting-out electrolyte has the meaning ascribed to in specification EP-A-79 646, that is salting-out electrolytes have a lyotropic number of less than 9.5.
• some salting-in electrolyte (as defined in the latter specification) may also be included, provided it is of a kind and in an amount compatible with the other components and the composition is still in accordance with the definition of the invention claimed herein.
• Some or all of the electrolyte may have detergency builder properties.
• compositions according to the present invention include detergency builder material, some or all of which may be electrolyte.
• the builder material is any capable of reducing the level of free calcium ions in the wash liquor and will preferably provide the composition with other beneficial properties such as the generation of an alkaline pH, the suspension of soil removed from the fabric and the dispersion of the fabric softening clay material.
• the salting-out electrolyte comprises citrate.
• Examples of phosphorus-containing inorganic detergency builders when present, include the water-soluble salts, especially alkali metal pyrophosphates, orthophosphates, polyphosphates and phosphonates.
• Specific examples of inorganic phosphate builders include sodium and potassium tripolyphosphates, phosphates and hexametaphosphates. Phosphonate sequestrant builders may also be used.
• non-phosphorus-containing inorganic detergency builders when present, include water-soluble alkali metal carbonates, bicarbonates, silicates and crystalline and amorphous aluminosilicates. Specific examples include sodium carbonate (with or without calcite seeds), potassium carbonate, sodium and potassium bicarbonates, silicates and zeolites.
• organic detergency builders when present, include the alkaline metal, ammonium and substituted ammonium polyacetates, carboxylates, polycarboxylates, polyacetyl carboxylates and polyhydroxysulphonates. Specific examples include sodium, potassium, lithium, ammonium and substituted ammonium salts of ethylenediaminetetraacetic acid, nitrilitriacetic acid, oxydisuccinic acid, CMOS, TMS, TDS, melitic acid, benzene polycarboxylic acids and citric acid.
• the level of non-soap builder material is from 0-50% by weight of the composition, more preferred from 5-40%, most preferred 10-35%.
• compositions of the present invention alternatively, or in addition to the partly dissolved polymer, yet another polymer which is substantially totally soluble in the aqueous phase and has an electrolyte resistance of more than 5 grams sodium nitrilotriacetate in 100ml of a 5% by weight aqueous solution of the polymer, said second polymer also having a vapour pressure in 20% aqueous solution, equal to or less than the vapour pressure of a reference 2% by weight or greater aqueous solution of polyethylene glycol having an average molecular weight of 6000; said second polymer having a molecular weight of at least 1000.
• Use of such polymers is generally described in our EP 301,883. Typical levels are from 0.5 to 4.5% by weight.
• compositions according to the present is preferably less than 1500 mPas, more preferred less than 1000 mPas, especially preferred between 30 and 900 mPas at 21 s- 1.
• One way of regulating the viscosity and stability of compositions according to the present invention is to include viscosity regulating polymeric materials.
• Viscosity and/or stability regulating polymers which are preferred for incorporation in compositions according to the invention include deflocculating polymers having a hydrophilic backbone and at least one hydrophobic side chain. Such polymers are for instance described in our co-pending European application EP 89201530.6 (EP 346 995).
• the amount of viscosity regulating polymer is from 0.1 to 5% by weight of the total composition, more preferred from 0.2 to 2%.
• compositions of the invention may also comprise materials for adjusting the pH.
• weak acids especially the use of organic acids is preferred, more preferred is the use of C 1 - 8 carboxylic acids, most preferred is the use of citric acid.
• bleach materials such as percarbonates or perborates, bleach precursors such as TAED, lather boosters such as alkanolamides, particularly the monoethanolamides derived from palm kernel fatty acids and coconut fatty acids, fabric softeners such as clays, amines and amine oxides, lather depressants, inorganic salts such as sodium sulphate, and, usually present in very minor amounts, fluorescent agents, perfumes, germicides colorants and other enzymes such as cellulases and amylases.
• bleach precursors such as TAED
• lather boosters such as alkanolamides, particularly the monoethanolamides derived from palm kernel fatty acids and coconut fatty acids
• fabric softeners such as clays, amines and amine oxides
• lather depressants such as inorganic salts such as sodium sulphate
• fluorescent agents such as perfumes, germicides colorants and other enzymes such as cellulases and amylases.
• Liquid compositions of the invention preferably comprise from 10 -80 % by weight of water, more preferably from 15- 60%, most preferably from 20-50 %.
• Liquid detergent compositions according to the invention are preferably physically stable in that they show less than 2% by volume phase separation upon storage for 21 days after preparation at 25 ° C.
• the detergent compositions of the invention will be diluted with wash water to form a wash liquor for instance for use in a washing machine.
• concentration of liquid detergent composition in the wash liquor is preferably from 0.05 to 10 %, more preferred from 0.1 to 3% by weight.
• the liquid detergent compositions preferably are alkaline, and it is preferred that they should provide a pH within the range of about 7.0 to 12, preferably about 8 to about 11, when used in aqueous solutions of the composition at the recommended concentration.
• the undiluted liquid composition should preferably be of a pH above 7, for example about pH 8.0 to about 12.5. It should be noted that an excessively high pH, e.g. over about pH 13, is less desirable for domestic safety.
• the pH is generally from 7.5 to 10.5, preferably 8 to 10, and especially 8.5 to 10, to ensure the combined effect of good detergency and good physical and chemical stability.
• the ingredients in any such highly alkaline detergent composition should, of course, be chosen for alkaline stability, especially for pH-sensitive materials such as enzymes, and a particularly suitable proteolytic enzyme.
• the pH may be adjusted by addition of a suitable alkaline or acid material.
• compositions according to the invention may be prepared by any method for the preparation of detergent compositions.
• a preferred method for the preparation of liquid detergent compositions of the invention comprises the addition of electrolyte materials -if any- to the water, followed by the addition of deflocculating polymers -if any-, the detergent active materials, the stabiliser material and the lipolytic enzyme as a premix and finally the remaining ingredients
• the lipolytic enzyme is preferably added as a premix with the stabiliser after addition of all ingredients (including the proteolytic enzyme if any).
• the K i of the stabilisation reaction between Lipolase TM (ex NOVO) and the stabiliser materials as indicated below was calculated from the K m values in the absence and presence of stabiliser.
• the K i was calculated from the K m values.
• compositions where prepared by adding the ingredients to the water in the following order: electrolytes, actives (as a premix), other ingredients except for lipase and stabiliser which were added as a premix as the final ingredient.
• Nonionic 1.2% synperonic A3, 3.6% synperonic A7.
• Lipase Lipolase SP400 (ex NOVO)
• Sample I contained lipase in the absence of savinase and stabilisers
• sample II contained Lipase and Savinase, but no stabiliser
• the remaining samples contained Lipase and Savinase and a stabiliser as indicated below.
• the level of Savinase in samples II-VIII was 10 GU/mg
• the level of stabiliser in samples III-VIII was 0.25 %.
• the half life time of the Lipase enzyme at 37 °C was determined for each sample the results were as follows: wherein:
• boronic acid derivatives are: octadecane boronic acid and phenyl, butyl boronic acid.
• the half life time of the lipase was determined at 37 °C.
• composition A the half life time in the absence of stabiliser was 1 day, in the presence of stabiliser 3 days.
• composition B the half life time in the absence of stabiliser was 14 days, in the presence of stabiliser 32 days.
• composition C the half-life time in the absence of stabiliser was 1 day, in the presence of stabiliser 2 days.

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• Engineering & Computer Science (AREA)
• Chemical Kinetics & Catalysis (AREA)
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• Wood Science & Technology (AREA)
• Organic Chemistry (AREA)
• Detergent Compositions (AREA)
• Enzymes And Modification Thereof (AREA)

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Cited By (29)

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Citations (6)

• 1991
  • 1991-09-16 EP EP91202359A patent/EP0478050A1/en not_active Withdrawn
  • 1991-09-20 AU AU84657/91A patent/AU8465791A/en not_active Abandoned
  • 1991-09-23 BR BR919104059A patent/BR9104059A/pt unknown
  • 1991-09-23 CA CA002052077A patent/CA2052077A1/en not_active Abandoned
  • 1991-09-23 NO NO91913733A patent/NO913733L/no unknown
  • 1991-09-24 JP JP3315608A patent/JPH04283298A/ja active Pending

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