EA201891721A1 - Регулирование экспрессии генов посредством аптамер-модулированного полиаденилирования - Google Patents

Регулирование экспрессии генов посредством аптамер-модулированного полиаденилирования

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Publication number
EA201891721A1
EA201891721A1 EA201891721A EA201891721A EA201891721A1 EA 201891721 A1 EA201891721 A1 EA 201891721A1 EA 201891721 A EA201891721 A EA 201891721A EA 201891721 A EA201891721 A EA 201891721A EA 201891721 A1 EA201891721 A1 EA 201891721A1
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EA
Eurasian Patent Office
Prior art keywords
gene expression
aptamer
polyadenylation
appamer
modulated
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EA201891721A
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English (en)
Inventor
Майкл Дж. Воллес
Оливье Ф. Дано
Сюэцуй Го
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МЕИРЭДжТиЭкс ЮКей II ЛИМИТЕД
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Publication of EA201891721A1 publication Critical patent/EA201891721A1/ru

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K48/0066—Manipulation of the nucleic acid to modify its expression pattern, e.g. enhance its duration of expression, achieved by the presence of particular introns in the delivered nucleic acid
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
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  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
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  • Virology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Developing Agents For Electrophotography (AREA)

Abstract

Изобретение предлагает полинуклеотидные конструкции для регуляции экспрессии генов с помощью основанной на аптамерах модуляции опосредованного малыми ядерными рибонуклеопротеинами (мяРНП) U1 подавления полиаденилирования и способы использования указанных конструкций для регуляции экспрессии генов в ответ на присутствие или отсутствие лиганда, который связывается с аптамером. Указанная полинуклеотидная конструкция содержит сайт связывания U1 в контексте рибопереключателя, содержащего эффекторную область и аптамер, такие, что, когда аптамер связывается с лигандом, происходит экспрессия гена-мишени.
EA201891721A 2016-02-02 2017-02-02 Регулирование экспрессии генов посредством аптамер-модулированного полиаденилирования EA201891721A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662290200P 2016-02-02 2016-02-02
PCT/US2017/016279 WO2017136591A1 (en) 2016-02-02 2017-02-02 Regulation of gene expression through aptamer-modulated polyadenylation

Publications (1)

Publication Number Publication Date
EA201891721A1 true EA201891721A1 (ru) 2019-02-28

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Family Applications (1)

Application Number Title Priority Date Filing Date
EA201891721A EA201891721A1 (ru) 2016-02-02 2017-02-02 Регулирование экспрессии генов посредством аптамер-модулированного полиаденилирования

Country Status (14)

Country Link
US (2) US11697815B2 (ru)
EP (1) EP3411081A4 (ru)
JP (1) JP7184649B2 (ru)
KR (1) KR20180117630A (ru)
CN (1) CN109511259B (ru)
AU (1) AU2017213835C1 (ru)
BR (1) BR112018015819A2 (ru)
CA (1) CA3048620A1 (ru)
EA (1) EA201891721A1 (ru)
MX (1) MX2018009370A (ru)
MY (1) MY197192A (ru)
PH (1) PH12018501650A1 (ru)
SG (1) SG11201806605RA (ru)
WO (1) WO2017136591A1 (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3005224A1 (en) * 2015-11-12 2017-05-18 Baylor College Of Medicine Exogenous control of mammalian gene expression through aptamer-mediated modulation of polyadenylation
MX2022011940A (es) 2020-03-24 2023-01-05 Meiragtx Uk Ii Ltd Aptámeros que se unen a análogos y derivados de la tiamina.
EP4179112A2 (en) 2020-07-13 2023-05-17 The Board of Trustees of the Leland Stanford Junior University Systems and methods to assess rna stability
EP4204015A2 (en) * 2020-08-31 2023-07-05 The Board of Trustees of the Leland Stanford Junior University Systems and methods for producing rna constructs with increased translation and stability
WO2023131811A2 (en) 2021-12-15 2023-07-13 Meiragtx Uk Ii Limited Polycistronic expression of gut peptides

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6692910B2 (en) 1997-07-28 2004-02-17 The University Of Connecticut Inhibition of a target messenger RNA with a modified U1 small nuclear RNA
US7563601B1 (en) 2005-06-01 2009-07-21 City Of Hope Artificial riboswitch for controlling pre-mRNA splicing
GB0701722D0 (en) * 2007-01-30 2007-03-07 Scottish Crop Res Inst Inhibition of gene expression
US9441221B2 (en) * 2007-03-30 2016-09-13 Rutgers, The State University Of New Jersey Compositions and methods for gene silencing
EP2142672B1 (en) * 2007-03-30 2012-09-05 Rutgers, The State University of New Jersey Compositions and methods for gene silencing
EP2430161A1 (en) * 2009-05-15 2012-03-21 Yale University Gemm riboswitches, structure-based compound design with gemm riboswitches, and methods and compositions for use of and with gemm riboswitches
US9125931B2 (en) 2010-04-06 2015-09-08 Massachusetts Institute Of Technology Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers
US11142762B2 (en) * 2012-07-05 2021-10-12 The Trustees Of The University Of Pennsylvania U1 snRNP regulates gene expression and modulates oncogenicity
WO2016126747A1 (en) * 2015-02-02 2016-08-11 Meiragtx Uk Limited Regulation of gene expression by aptamer-mediated modulation of alternative splicing
HRP20240183T1 (hr) * 2016-08-03 2024-04-26 Meiragtx Uk Ii Limited Stanični probir visoke propusnosti za aptamere

Also Published As

Publication number Publication date
JP7184649B2 (ja) 2022-12-06
CN109511259B (zh) 2023-10-20
PH12018501650A1 (en) 2019-06-03
US20200017858A1 (en) 2020-01-16
EP3411081A4 (en) 2019-12-11
BR112018015819A2 (pt) 2019-01-02
EP3411081A1 (en) 2018-12-12
AU2017213835A1 (en) 2018-09-06
KR20180117630A (ko) 2018-10-29
JP2019503204A (ja) 2019-02-07
CN109511259A (zh) 2019-03-22
MY197192A (en) 2023-05-31
AU2017213835C1 (en) 2024-04-18
US11697815B2 (en) 2023-07-11
CA3048620A1 (en) 2017-08-10
US20240191239A1 (en) 2024-06-13
SG11201806605RA (en) 2018-09-27
MX2018009370A (es) 2018-11-12
WO2017136591A1 (en) 2017-08-10
AU2017213835B2 (en) 2024-02-01

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