EA201690271A1 - Конструкции для экспрессии и способы экспрессии полипептидов в клетках эукариот - Google Patents
Конструкции для экспрессии и способы экспрессии полипептидов в клетках эукариотInfo
- Publication number
- EA201690271A1 EA201690271A1 EA201690271A EA201690271A EA201690271A1 EA 201690271 A1 EA201690271 A1 EA 201690271A1 EA 201690271 A EA201690271 A EA 201690271A EA 201690271 A EA201690271 A EA 201690271A EA 201690271 A1 EA201690271 A1 EA 201690271A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- expression
- cells
- eukaryot
- constructions
- methods
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/46—Hybrid immunoglobulins
- C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
- C12N2840/445—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor for trans-splicing, e.g. polypyrimidine tract, branch point splicing
Abstract
Настоящее изобретение относится к экспрессируемой конструкции для экспрессии полипептидов в клетках-хозяевах с использованием альтернативного сплайсинга. Конструкцию для экспрессии можно применять для экспрессии полипептидов, таких как антитела, фрагменты антител и биспецифичные антитела, путем экспрессии продуктов генов, необходимых для экспрессии белка, в соотношении, обеспечивающем получение самых высоких титров или самого лучшего профиля качества продукта.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP13179375 | 2013-08-06 | ||
PCT/EP2014/066826 WO2015018832A1 (en) | 2013-08-06 | 2014-08-05 | Expression constructs and methods for expressing polypeptides in eukaryotic cells |
Publications (1)
Publication Number | Publication Date |
---|---|
EA201690271A1 true EA201690271A1 (ru) | 2016-07-29 |
Family
ID=51300736
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201690271A EA201690271A1 (ru) | 2013-08-06 | 2014-08-05 | Конструкции для экспрессии и способы экспрессии полипептидов в клетках эукариот |
Country Status (14)
Country | Link |
---|---|
US (3) | US20150056655A1 (ru) |
EP (1) | EP3030579A1 (ru) |
JP (3) | JP2016528896A (ru) |
KR (2) | KR102104581B1 (ru) |
CN (1) | CN105658665A (ru) |
AU (2) | AU2014304570B2 (ru) |
BR (1) | BR112016002319A2 (ru) |
CA (1) | CA2920574C (ru) |
EA (1) | EA201690271A1 (ru) |
IL (2) | IL243967A0 (ru) |
MX (1) | MX2016001678A (ru) |
NZ (1) | NZ717178A (ru) |
SG (1) | SG11201600736SA (ru) |
WO (1) | WO2015018832A1 (ru) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017041004A1 (en) * | 2015-09-02 | 2017-03-09 | The Regents Of The University Of Colorado, A Body Corporate | Compositions and methods for modulating t-cell mediated immune response |
WO2020205604A1 (en) * | 2019-03-29 | 2020-10-08 | Salk Institute For Biological Studies | High-efficiency reconstitution of rna molecules |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6136566A (en) * | 1996-10-04 | 2000-10-24 | Lexicon Graphics Incorporated | Indexed library of cells containing genomic modifications and methods of making and utilizing the same |
US20040072243A1 (en) * | 1996-10-11 | 2004-04-15 | Lexicon Genetics Incorporated | Indexed library of cells containing genomic modifications and methods of making and utilizing the same |
WO2005089285A2 (en) * | 2004-03-15 | 2005-09-29 | Biogen Idec Ma Inc. | Methods and constructs for expressing polypeptide multimers in eukaryotic cells using alternative splicing |
US20090305343A1 (en) * | 2006-05-16 | 2009-12-10 | Millegen | Method For Expressing Polypeptides In Eukaryotic Cells Using Alternative Splicing |
JP5875009B2 (ja) * | 2010-06-01 | 2016-03-02 | 国立大学法人京都大学 | 哺乳類生物における選択的スプライシングの発現プロファイルおよび制御機構を明らかにするトランスジェニックレポーターシステム |
-
2014
- 2014-08-05 BR BR112016002319A patent/BR112016002319A2/pt not_active Application Discontinuation
- 2014-08-05 EP EP14749786.1A patent/EP3030579A1/en not_active Withdrawn
- 2014-08-05 EA EA201690271A patent/EA201690271A1/ru unknown
- 2014-08-05 NZ NZ717178A patent/NZ717178A/en unknown
- 2014-08-05 WO PCT/EP2014/066826 patent/WO2015018832A1/en active Application Filing
- 2014-08-05 KR KR1020167006036A patent/KR102104581B1/ko active IP Right Grant
- 2014-08-05 CN CN201480055196.2A patent/CN105658665A/zh active Pending
- 2014-08-05 AU AU2014304570A patent/AU2014304570B2/en active Active
- 2014-08-05 CA CA2920574A patent/CA2920574C/en active Active
- 2014-08-05 KR KR1020207011393A patent/KR20200044154A/ko not_active Application Discontinuation
- 2014-08-05 JP JP2016532665A patent/JP2016528896A/ja active Pending
- 2014-08-05 MX MX2016001678A patent/MX2016001678A/es unknown
- 2014-08-05 SG SG11201600736SA patent/SG11201600736SA/en unknown
- 2014-08-06 US US14/453,328 patent/US20150056655A1/en not_active Abandoned
-
2016
- 2016-02-04 IL IL243967A patent/IL243967A0/en unknown
- 2016-11-17 US US15/354,907 patent/US20170253671A1/en not_active Abandoned
-
2019
- 2019-07-16 US US16/512,482 patent/US20200172634A1/en active Pending
- 2019-09-10 IL IL26925219A patent/IL269252A/en unknown
- 2019-09-23 AU AU2019236586A patent/AU2019236586B2/en active Active
-
2020
- 2020-07-17 JP JP2020122925A patent/JP2020202840A/ja active Pending
-
2022
- 2022-09-12 JP JP2022144740A patent/JP2022177131A/ja active Pending
Also Published As
Publication number | Publication date |
---|---|
EP3030579A1 (en) | 2016-06-15 |
AU2019236586B2 (en) | 2020-12-03 |
CA2920574C (en) | 2021-03-16 |
CA2920574A1 (en) | 2015-02-12 |
US20150056655A1 (en) | 2015-02-26 |
KR20200044154A (ko) | 2020-04-28 |
JP2020202840A (ja) | 2020-12-24 |
KR20160035084A (ko) | 2016-03-30 |
AU2014304570B2 (en) | 2019-07-25 |
AU2019236586A1 (en) | 2019-10-10 |
KR102104581B1 (ko) | 2020-06-02 |
SG11201600736SA (en) | 2016-02-26 |
CN105658665A (zh) | 2016-06-08 |
NZ717178A (en) | 2022-02-25 |
MX2016001678A (es) | 2016-10-28 |
JP2016528896A (ja) | 2016-09-23 |
BR112016002319A2 (pt) | 2017-09-12 |
JP2022177131A (ja) | 2022-11-30 |
IL243967A0 (en) | 2016-04-21 |
US20170253671A1 (en) | 2017-09-07 |
IL269252A (en) | 2019-11-28 |
WO2015018832A1 (en) | 2015-02-12 |
AU2014304570A1 (en) | 2016-03-10 |
US20200172634A1 (en) | 2020-06-04 |
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