SG11201600736SA - Expression constructs and methods for expressing polypeptides in eukaryotic cells - Google Patents

Expression constructs and methods for expressing polypeptides in eukaryotic cells

Info

Publication number
SG11201600736SA
SG11201600736SA SG11201600736SA SG11201600736SA SG11201600736SA SG 11201600736S A SG11201600736S A SG 11201600736SA SG 11201600736S A SG11201600736S A SG 11201600736SA SG 11201600736S A SG11201600736S A SG 11201600736SA SG 11201600736S A SG11201600736S A SG 11201600736SA
Authority
SG
Singapore
Prior art keywords
methods
eukaryotic cells
expression constructs
expressing polypeptides
polypeptides
Prior art date
Application number
SG11201600736SA
Inventor
Christel Aebischer-Gumy
Martin Bertschinger
Pierre Moretti
Original Assignee
Glenmark Pharmaceuticals Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glenmark Pharmaceuticals Sa filed Critical Glenmark Pharmaceuticals Sa
Publication of SG11201600736SA publication Critical patent/SG11201600736SA/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • C07K16/468Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00Preparation of peptides or proteins
    • C12P21/02Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/10Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14Specific host cells or culture conditions, e.g. components, pH or temperature
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622Single chain antibody (scFv)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/60Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/44Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
    • C12N2840/445Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor for trans-splicing, e.g. polypyrimidine tract, branch point splicing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
SG11201600736SA 2013-08-06 2014-08-05 Expression constructs and methods for expressing polypeptides in eukaryotic cells SG11201600736SA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13179375 2013-08-06
PCT/EP2014/066826 WO2015018832A1 (en) 2013-08-06 2014-08-05 Expression constructs and methods for expressing polypeptides in eukaryotic cells

Publications (1)

Publication Number Publication Date
SG11201600736SA true SG11201600736SA (en) 2016-02-26

Family

ID=51300736

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201600736SA SG11201600736SA (en) 2013-08-06 2014-08-05 Expression constructs and methods for expressing polypeptides in eukaryotic cells

Country Status (14)

Country Link
US (3) US20150056655A1 (en)
EP (1) EP3030579A1 (en)
JP (3) JP2016528896A (en)
KR (2) KR20200044154A (en)
CN (1) CN105658665A (en)
AU (2) AU2014304570B2 (en)
BR (1) BR112016002319A2 (en)
CA (1) CA2920574C (en)
EA (1) EA201690271A1 (en)
IL (2) IL243967A0 (en)
MX (1) MX2016001678A (en)
NZ (1) NZ717178A (en)
SG (1) SG11201600736SA (en)
WO (1) WO2015018832A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4223784A3 (en) * 2015-09-02 2023-10-04 The Regents of the University of Colorado, a body corporate Compositions and methods for modulating t-cell mediated immune response
WO2020205604A1 (en) * 2019-03-29 2020-10-08 Salk Institute For Biological Studies High-efficiency reconstitution of rna molecules

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136566A (en) * 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
CN1961071A (en) * 2004-03-15 2007-05-09 比奥根艾迪克Ma公司 Methods and constructs for expressing polypeptide polymers in eukaryotic cells using alternative splicing
EP2018430A1 (en) * 2006-05-16 2009-01-28 Millegen Method for expressing polypeptides in eukaryotic cells using alternative splicing
EP2576778B1 (en) * 2010-06-01 2017-08-09 Kyoto University Transgenic reporter system that reveals expression profiles and regulation mechanisms of alternative splicing in rodents

Also Published As

Publication number Publication date
EP3030579A1 (en) 2016-06-15
NZ717178A (en) 2022-02-25
JP2022177131A (en) 2022-11-30
AU2019236586A1 (en) 2019-10-10
US20170253671A1 (en) 2017-09-07
AU2019236586B2 (en) 2020-12-03
BR112016002319A2 (en) 2017-09-12
CA2920574C (en) 2021-03-16
MX2016001678A (en) 2016-10-28
KR20160035084A (en) 2016-03-30
US20150056655A1 (en) 2015-02-26
US20200172634A1 (en) 2020-06-04
EA201690271A1 (en) 2016-07-29
WO2015018832A1 (en) 2015-02-12
CN105658665A (en) 2016-06-08
AU2014304570B2 (en) 2019-07-25
JP2020202840A (en) 2020-12-24
KR20200044154A (en) 2020-04-28
JP2016528896A (en) 2016-09-23
IL269252A (en) 2019-11-28
AU2014304570A1 (en) 2016-03-10
KR102104581B1 (en) 2020-06-02
CA2920574A1 (en) 2015-02-12
IL243967A0 (en) 2016-04-21

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