EA201390765A1 - Событие gat dp-073496-4 brassica и композиции и способы для его идентификации и/или детектирования - Google Patents

Событие gat dp-073496-4 brassica и композиции и способы для его идентификации и/или детектирования

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Publication number
EA201390765A1
EA201390765A1 EA201390765A EA201390765A EA201390765A1 EA 201390765 A1 EA201390765 A1 EA 201390765A1 EA 201390765 A EA201390765 A EA 201390765A EA 201390765 A EA201390765 A EA 201390765A EA 201390765 A1 EA201390765 A1 EA 201390765A1
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Eurasian Patent Office
Prior art keywords
event
compositions
methods
transgene
genome
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EA201390765A
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English (en)
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EA031629B1 (ru
Inventor
Дэвид Джордж Шарн
Вэньпинь Чэнь
Чэдвик Брюс Косайелни
Джаянтилал Девабхай Пател
Фердинанд Джерард Тунен
Ломас Тульзиерам
Юнпин Чжан
Чжунсэнь Ли
Original Assignee
Пайонир Хай-Бред Интернэшнл, Инк.
Е. И. Дю Пон Де Немур Энд Компани
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Application filed by Пайонир Хай-Бред Интернэшнл, Инк., Е. И. Дю Пон Де Немур Энд Компани filed Critical Пайонир Хай-Бред Интернэшнл, Инк.
Publication of EA201390765A1 publication Critical patent/EA201390765A1/ru
Publication of EA031629B1 publication Critical patent/EA031629B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C12N15/8275—Glyphosate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/91045—Acyltransferases (2.3)
    • G01N2333/91051—Acyltransferases other than aminoacyltransferases (general) (2.3.1)

Abstract

Представлены композиции и способы, относящиеся к трансгенным, устойчивым к глифосату растениям Brassica. Конкретно, настоящее изобретение относится к растениям Brassica, содержащим событие DP-073496-4, которое обеспечивает устойчивость к глифосату. Растение Brassica, несущее событие DP-073496-4 в указанной хромосомной локализации, включает участки соединения геном/трансген в SEQ ID NO: 2 или участки соединения геном/трансген, приведенные в SEQ ID NO: 12 и/или 13. Характеристика вставок события в геномный участок представляет повышенную эффективность разведения и обеспечивает применение молекулярных маркеров для отслеживания трансгенной вставки в разводимых популяциях и их потомстве. Представлены различные способы и композиции для идентификации, детектирования и применения события.
EA201390765A 2010-11-24 2010-11-24 Событие gat dp-073496-4 brassica и композиции и способы для его идентификации и/или детектирования EA031629B1 (ru)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2010/058011 WO2012071040A1 (en) 2010-11-24 2010-11-24 Brassica gat event dp-073496-4 and compositions and methods for the identification and/or detection thereof

Publications (2)

Publication Number Publication Date
EA201390765A1 true EA201390765A1 (ru) 2013-11-29
EA031629B1 EA031629B1 (ru) 2019-01-31

Family

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EA201390765A EA031629B1 (ru) 2010-11-24 2010-11-24 Событие gat dp-073496-4 brassica и композиции и способы для его идентификации и/или детектирования

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EP (1) EP2643464B1 (ru)
CN (2) CN107815462A (ru)
AU (1) AU2010364322C1 (ru)
CA (1) CA2810180C (ru)
EA (1) EA031629B1 (ru)
HU (1) HUE043280T2 (ru)
PL (1) PL2643464T3 (ru)
UA (1) UA122558C2 (ru)
WO (1) WO2012071040A1 (ru)

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WO2013177427A1 (en) * 2012-05-23 2013-11-28 E. I. Du Pont De Nemours And Company Brassica genomic assays
AU2014241045B2 (en) 2013-03-13 2017-08-31 Pioneer Hi-Bred International, Inc. Glyphosate application for weed control in brassica
US10329578B2 (en) 2013-10-18 2019-06-25 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use
CN106780077A (zh) * 2016-11-23 2017-05-31 北海高创电子信息孵化器有限公司 一种草本植物抗药性信息收集和整治系统的工作方法
CN106815766A (zh) * 2016-11-23 2017-06-09 北海高创电子信息孵化器有限公司 一种草本植物抗药性信息收集和整治系统
CN106701999B (zh) * 2017-02-24 2019-01-04 四川省农业科学院分析测试中心 含有gat-tpinⅡ结构转基因植物实时PCR精准检测的引物和探针及方法
CN108486008B (zh) * 2018-03-26 2021-08-24 延边大学 对鳞翅目害虫高毒力的苏云金杆菌yn108、培养方法及其应用
CN109825634A (zh) * 2019-04-03 2019-05-31 深圳出入境检验检疫局食品检验检疫技术中心 转基因油菜品系dp-073496-4检测方法和试剂
CN111543309A (zh) * 2020-05-19 2020-08-18 吉林省农业科学院 一种牧草的育种方法
CN114586797B (zh) * 2020-12-03 2023-09-01 青岛清原作物科学有限公司 包含灭草松和砜嘧磺隆的三元除草组合物及其应用
CA3201992A1 (en) 2020-12-21 2022-06-30 Thi Ninh Thuan NGUYEN Brassica napus plants comprising an improved fertility restorer

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Publication number Publication date
CN103748227A (zh) 2014-04-23
UA122558C2 (uk) 2020-12-10
EP2643464B1 (en) 2018-12-26
CA2810180A1 (en) 2012-05-31
HUE043280T2 (hu) 2019-08-28
AU2010364322A1 (en) 2013-02-28
AU2010364322B2 (en) 2013-03-21
WO2012071040A1 (en) 2012-05-31
EP2643464A1 (en) 2013-10-02
EA031629B1 (ru) 2019-01-31
AU2010364322C1 (en) 2013-09-19
PL2643464T3 (pl) 2019-08-30
CN107815462A (zh) 2018-03-20
CA2810180C (en) 2015-07-28

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