EA201190109A1 - Изолированные популяции моноцитов и связанные с ними терапевтические применения - Google Patents
Изолированные популяции моноцитов и связанные с ними терапевтические примененияInfo
- Publication number
- EA201190109A1 EA201190109A1 EA201190109A EA201190109A EA201190109A1 EA 201190109 A1 EA201190109 A1 EA 201190109A1 EA 201190109 A EA201190109 A EA 201190109A EA 201190109 A EA201190109 A EA 201190109A EA 201190109 A1 EA201190109 A1 EA 201190109A1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- monocytes
- sample
- isolated
- red blood
- blood cells
- Prior art date
Links
- 210000001616 monocyte Anatomy 0.000 title abstract 7
- 230000001225 therapeutic effect Effects 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 3
- 210000003743 erythrocyte Anatomy 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 208000030533 eye disease Diseases 0.000 abstract 2
- 101000946889 Homo sapiens Monocyte differentiation antigen CD14 Proteins 0.000 abstract 1
- 101000934338 Homo sapiens Myeloid cell surface antigen CD33 Proteins 0.000 abstract 1
- 102100035877 Monocyte differentiation antigen CD14 Human genes 0.000 abstract 1
- 102100025243 Myeloid cell surface antigen CD33 Human genes 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000001185 bone marrow Anatomy 0.000 abstract 1
- 230000003412 degenerative effect Effects 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- 230000002792 vascular Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0645—Macrophages, e.g. Kuepfer cells in the liver; Monocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/461—Cellular immunotherapy characterised by the cell type used
- A61K39/4614—Monocytes; Macrophages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/462—Cellular immunotherapy characterized by the effect or the function of the cells
- A61K39/4622—Antigen presenting cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/46—Cellular immunotherapy
- A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P27/00—Drugs for disorders of the senses
- A61P27/02—Ophthalmic agents
- A61P27/06—Antiglaucoma agents or miotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P9/00—Drugs for disorders of the cardiovascular system
- A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
- A61K2239/31—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterized by the route of administration
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/05—Adjuvants
- C12N2501/051—Lipid A (MPA, MPL)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/05—Adjuvants
- C12N2501/052—Lipopolysaccharides [LPS]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/21—Chemokines, e.g. MIP-1, MIP-2, RANTES, MCP, PF-4
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/04—Investigating sedimentation of particle suspensions
- G01N15/042—Investigating sedimentation of particle suspensions by centrifuging and investigating centrifugates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1497—Particle shape
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Epidemiology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Ophthalmology & Optometry (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Hematology (AREA)
- General Engineering & Computer Science (AREA)
- Vascular Medicine (AREA)
- Virology (AREA)
- Urology & Nephrology (AREA)
- Cardiology (AREA)
- Developmental Biology & Embryology (AREA)
- Heart & Thoracic Surgery (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Согласно настоящему изобретению предложены способы применения изолированных популяций моноцитов для лечения субъектов, страдающих от различных сосудистых заболеваний глаз или дегенеративных расстройств глаз. Согласно настоящему изобретению также предложены новые способы выделения, по существу, чистых популяций моноцитов. Указанные способы включают получение образца крови или образца костного мозга от субъекта, уменьшение количества красных кровяных клеток в указанном образце, а затем отделение оставшихся в образце красных кровяных клеток и других типов клеток от моноцитов. Вместо применения каких-либо селективных или вводящих метку агентов красные кровяные клетки и другие типы клеток отделяют от моноцитов на основании их размера, гранулярности или плотности. Изолированные моноциты можно дополнительно активировать in vitro или ex vivo перед введением субъекту. Согласно настоящему изобретению также предложены изолированные популяции клеток, содержащие, по существу, чистые моноциты CD14/CD33.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US20817309P | 2009-02-20 | 2009-02-20 | |
US28324409P | 2009-11-30 | 2009-11-30 | |
PCT/US2010/000477 WO2010096177A1 (en) | 2009-02-20 | 2010-02-19 | Isolated monocyte populations and related therapeutic applications |
Publications (1)
Publication Number | Publication Date |
---|---|
EA201190109A1 true EA201190109A1 (ru) | 2012-07-30 |
Family
ID=42634163
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201190109A EA201190109A1 (ru) | 2009-02-20 | 2010-02-19 | Изолированные популяции моноцитов и связанные с ними терапевтические применения |
Country Status (11)
Country | Link |
---|---|
EP (1) | EP2398900A4 (ru) |
JP (1) | JP2012518407A (ru) |
KR (1) | KR20110123778A (ru) |
CN (1) | CN102317446A (ru) |
AU (1) | AU2010216374A1 (ru) |
BR (1) | BRPI1008392A2 (ru) |
CA (1) | CA2752679A1 (ru) |
EA (1) | EA201190109A1 (ru) |
MX (1) | MX2011008826A (ru) |
WO (1) | WO2010096177A1 (ru) |
ZA (1) | ZA201105869B (ru) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2913274C (en) * | 2013-05-22 | 2021-10-12 | Yeda Research And Development Co. Ltd. | Human monocyte sub-population for treatment of eye diseases and disorders |
KR101668462B1 (ko) * | 2015-02-26 | 2016-10-24 | 충북대학교 산학협력단 | 혈액성분을 유효성분으로 포함하는 녹내장의 예방 또는 치료용 약제학적 조성물 |
FR3084463B1 (fr) * | 2018-07-30 | 2023-04-21 | Francais Du Sang Ets | Procede d'analyse des plaquettes d'un echantillon sanguin |
CN114441419B (zh) * | 2022-01-29 | 2022-11-22 | 杭州翔宇医学检验实验室有限公司 | 流式圈门方法及应用 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2006031467A2 (en) * | 2002-07-25 | 2006-03-23 | The Scripps Research Institute | Isolated lineage negative hematopoietic stem cells and methods of treatment therewith |
CA2556018A1 (en) * | 2004-02-11 | 2005-09-09 | Aldagen, Inc. | Stem cell populations and methods of use |
US20070231306A1 (en) * | 2005-02-24 | 2007-10-04 | The Scripps Research Institute | Isolated myeloid-like cell populations and methods of treatment therewith |
JP5412280B2 (ja) * | 2006-06-28 | 2014-02-12 | イエダ リサーチ アンド デベロップメント カンパニー リミテッド | 加齢性黄斑変性症の治療法 |
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2010
- 2010-02-19 WO PCT/US2010/000477 patent/WO2010096177A1/en active Application Filing
- 2010-02-19 AU AU2010216374A patent/AU2010216374A1/en not_active Abandoned
- 2010-02-19 CN CN2010800079738A patent/CN102317446A/zh active Pending
- 2010-02-19 MX MX2011008826A patent/MX2011008826A/es not_active Application Discontinuation
- 2010-02-19 CA CA2752679A patent/CA2752679A1/en not_active Abandoned
- 2010-02-19 KR KR1020117021989A patent/KR20110123778A/ko not_active Application Discontinuation
- 2010-02-19 JP JP2011551065A patent/JP2012518407A/ja not_active Withdrawn
- 2010-02-19 EP EP10744061A patent/EP2398900A4/en not_active Withdrawn
- 2010-02-19 BR BRPI1008392-8A patent/BRPI1008392A2/pt not_active IP Right Cessation
- 2010-02-19 EA EA201190109A patent/EA201190109A1/ru unknown
-
2011
- 2011-08-11 ZA ZA2011/05869A patent/ZA201105869B/en unknown
Also Published As
Publication number | Publication date |
---|---|
WO2010096177A1 (en) | 2010-08-26 |
MX2011008826A (es) | 2011-11-02 |
BRPI1008392A2 (pt) | 2015-08-25 |
KR20110123778A (ko) | 2011-11-15 |
CA2752679A1 (en) | 2010-08-26 |
JP2012518407A (ja) | 2012-08-16 |
CN102317446A (zh) | 2012-01-11 |
EP2398900A1 (en) | 2011-12-28 |
EP2398900A4 (en) | 2012-08-22 |
ZA201105869B (en) | 2012-10-31 |
AU2010216374A1 (en) | 2011-08-18 |
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