EA201100802A1 - Синтез пептидов с использованием энзиматической активации и конденсации - Google Patents

Синтез пептидов с использованием энзиматической активации и конденсации

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Publication number
EA201100802A1
EA201100802A1 EA201100802A EA201100802A EA201100802A1 EA 201100802 A1 EA201100802 A1 EA 201100802A1 EA 201100802 A EA201100802 A EA 201100802A EA 201100802 A EA201100802 A EA 201100802A EA 201100802 A1 EA201100802 A1 EA 201100802A1
Authority
EA
Eurasian Patent Office
Prior art keywords
terminally protected
reaction medium
hybridized carbon
amino acid
ester
Prior art date
Application number
EA201100802A
Other languages
English (en)
Other versions
EA019536B1 (ru
Inventor
Петер Ян Леонард Марио Квадфлиг
Тимо Нёйенс
Клаудиа Кузан
Катерина Хюбертина Мария Шеперс
Original Assignee
ДСМ АйПи АССЕТС Б.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ДСМ АйПи АССЕТС Б.В. filed Critical ДСМ АйПи АССЕТС Б.В.
Publication of EA201100802A1 publication Critical patent/EA201100802A1/ru
Publication of EA019536B1 publication Critical patent/EA019536B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C07K1/026—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution by fragment condensation in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/10—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using coupling agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Способ энзиматического синтеза пептидов, включающий энзиматическое получение эфира или тиоэфира из (i) защищенной с N-конца аминокислоты, С-концевого эфира защищенной с N-конца аминокислоты, защищенного с N-конца пептида или С-концевого эфира защищенного с N-конца пептида и (ii) спирта, представленного формулой HO-CX-Z, или же тиола, представленного формулой HS-CX-Z, где каждый Х независимо означает атом галогена или атом водорода; a Z выбирается из группы sp-гибридизованных атомов углерода, содержащих по меньшей мере два заместителя, содержащих гетероатом, присоединенный непосредственно к sp-гибридизованному углероду, и sp-гибридизованных атомов углерода, содержащих один или два заместителя, содержащих гетероатом, присоединенный непосредственно к sp-гибридизованному углероду, причем получение эфира или тиоэфира осуществляется в реакционной среде, содержащей 2 мас.% или меньше воды, исходя из массы жидкости в реакционной среде; и энзиматическое присоединение полученного эфира или тиоэфира к необязательно защищенной с С-конца аминокислоте или необязательно защищенному с С-конца пептиду, при этом пептид синтезируется в реакционной среде, содержащей 2 мас.% или меньше воды, исходя из общей массы реакционной среды.
EA201100802A 2008-11-19 2009-11-19 Синтез пептидов с использованием энзиматической активации и конденсации EA019536B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP08169425 2008-11-19
PCT/EP2009/065512 WO2010057961A1 (en) 2008-11-19 2009-11-19 Peptide synthesis using enzymatic activation and coupling

Publications (2)

Publication Number Publication Date
EA201100802A1 true EA201100802A1 (ru) 2011-12-30
EA019536B1 EA019536B1 (ru) 2014-04-30

Family

ID=40848060

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201100802A EA019536B1 (ru) 2008-11-19 2009-11-19 Синтез пептидов с использованием энзиматической активации и конденсации

Country Status (9)

Country Link
US (1) US8883444B2 (ru)
EP (1) EP2365982A1 (ru)
JP (1) JP2012509089A (ru)
KR (1) KR20110088519A (ru)
CN (1) CN102216324B (ru)
BR (1) BRPI0921081A2 (ru)
CA (1) CA2744243A1 (ru)
EA (1) EA019536B1 (ru)
WO (1) WO2010057961A1 (ru)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2634193B1 (en) * 2012-02-29 2015-01-14 Enzypep B.V. Side-chain protected oligopeptide fragment condensation using subtilisins in organic solvents
WO2013135786A1 (en) * 2012-03-14 2013-09-19 Dsm Fine Chemicals Austria Nfg. Gmbh & Co Kg Enzymatic peptide synthesis
CN107002110A (zh) * 2014-10-10 2017-08-01 恩细贝普有限公司 用具有改善的合成水解比的枯草杆菌蛋白酶变体的肽片段缩合和环化
EP3299085A1 (en) 2016-09-26 2018-03-28 Enzypep B.V. Peptide coupling molecule
CN115884963A (zh) 2020-06-25 2023-03-31 人福医药美国公司 用于治疗医学病症的肽

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5272885A (en) * 1975-10-23 1977-06-17 Sagami Chem Res Center Preparation of peptide
JPH06237784A (ja) * 1993-02-18 1994-08-30 Daiwa Kasei Kk オリゴペプチドの合成法
NL1005901C2 (nl) * 1997-04-25 1998-10-27 Dsm Nv Werkwijze voor de verestering van aminozuren en peptiden.
DE602006006326D1 (de) * 2005-10-20 2009-05-28 Dsm Ip Assets Bv Enzymatische umsetzung von oligopeptidamiden zu oligopeptidalkylestern
WO2009047354A1 (en) * 2007-10-12 2009-04-16 Dsm Ip Assets B.V. Chemo-enzymatic synthesis of a c-terminal thioester of an amino acid or a peptide
WO2009047311A1 (en) * 2007-10-12 2009-04-16 Dsm Ip Assets B.V. Chemo-enzymatic peptide synthesis via c-terminal ester interconversion

Also Published As

Publication number Publication date
CN102216324B (zh) 2014-02-12
BRPI0921081A2 (pt) 2015-12-15
WO2010057961A1 (en) 2010-05-27
CA2744243A1 (en) 2010-05-27
US20120129214A1 (en) 2012-05-24
CN102216324A (zh) 2011-10-12
KR20110088519A (ko) 2011-08-03
EP2365982A1 (en) 2011-09-21
JP2012509089A (ja) 2012-04-19
EA019536B1 (ru) 2014-04-30
US8883444B2 (en) 2014-11-11

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Legal Events

Date Code Title Description
MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM RU